Publications

mycoplasma genitalium infection is associated with microscopic signs of cervical inflammation in liquid cytology specimens.	cervicitis is a common clinical finding often attributed to sexually transmitted infections (stis), but no etiologic agent is identified in the majority of cases. in this study, we comparatively assessed inflammation among the common infectious etiologies of cervicitis and assessed the potential value of liquid cytology specimens for predicting stis. among 473 louisiana women at low risk for acquiring stis, the prevalences of mycoplasma genitalium, chlamydia trachomatis, neisseria gonorrhoeae, a ...	2014	24759719
hela nucleic acid contamination in the cancer genome atlas leads to the misidentification of human papillomavirus 18.	we searched the cancer genome atlas (tcga) database for viruses by comparing non-human reads present in transcriptome sequencing (rna-seq) and whole-exome sequencing (wxs) data to viral sequence databases. human papillomavirus 18 (hpv18) is an etiologic agent of cervical cancer, and as expected, we found robust expression of hpv18 genes in cervical cancer samples. in agreement with previous studies, we also found hpv18 transcripts in non-cervical cancer samples, including those from the colon, r ...	2015	25631090
human papillomavirus 18 genetic variation and cervical cancer risk worldwide.	human papillomavirus 18 (hpv18) is the second most carcinogenic hpv type, after hpv16, and it accounts for approximately 12% of squamous cell carcinoma (scc) as well as 37% of adenocarcinoma (adc) of the cervix worldwide. we aimed to evaluate the worldwide diversity and carcinogenicity of hpv18 genetic variants by sequencing the entire long control region (lcr) and the e6 open reading frame of 711 hpv18-positive cervical samples from 39 countries, taking advantage of the international agency for ...	2015	26269181
in silico docking to explicate interface between plant-originated inhibitors and e6 oncogenic protein of highly threatening human papillomavirus 18.	the leading cause of cancer mortality globally amongst the women is due to human papillomavirus (hpv) infection. there is need to explore anti-cancerous drugs against this life-threatening infection. traditionally, different natural compounds such as withaferin a, artemisinin, ursolic acid, ferulic acid, (-)-epigallocatechin-3-gallate, berberin, resveratrol, jaceosidin, curcumin, gingerol, indol-3-carbinol, and silymarin have been used as hopeful source of cancer treatment. these natural inhibit ...	2015	26175664
gene silencing and activation of human papillomavirus 18 is modulated by sense promoter associated rna in bidirectionally transcribed long control region.	recently various studies have demonstrated the role of promoter associated non-coding rnas (prna) in dsrna induced transcriptional gene silencing and activation. however the exact mechanistic details of these processes with respect to the orientation of prnas are poorly defined.	2015	26047143
the nuclear dna sensor ifi16 acts as a restriction factor for human papillomavirus replication through epigenetic modifications of the viral promoters.	the human interferon-inducible ifi16 protein, an innate immune sensor of intracellular dna, was recently demonstrated to act as a restriction factor for human cytomegalovirus (hcmv) and herpes simplex virus 1 (hsv-1) infection by inhibiting both viral-dna replication and transcription. through the use of two distinct cellular models, this study provides strong evidence in support of the notion that ifi16 can also restrict human papillomavirus 18 (hpv18) replication. in the first model, an immort ...	2015	25972554
a novel dithiocarbamate derivative induces cell apoptosis through p53-dependent intrinsic pathway and suppresses the expression of the e6 oncogene of human papillomavirus 18 in hela cells.	dithiocarbamates (dtcs) exhibit a broad spectrum of antitumor activities, however, their molecular mechanisms of antitumor have not yet been elucidated. previously, we have synthesized a series of novel dithiocarbamate derivatives. these dtcs were examined for cytotoxic activities against five human cancer cell lines. in this study, one of dithiocarbamate (dtc1) with higher potential for hela cells was chosen to investigate molecular mechanisms for its anti-tumor activities. dtc1 could inhibit p ...	2015	25772545
inconclusive evidence for non-inferior immunogenicity of two- compared with three-dose hpv immunization schedules in preadolescent girls: a systematic review and meta-analysis.	the european medicines agency (ema) recently approved two-dose immunization schedules for bivalent (hpv 16/18) and quadrivalent (hpv 6/11/16/18) human papillomavirus (hpv) vaccines in nine to fourteen and thirteen year-old-girls, respectively. registration was based on trials comparing immunogenicity of two-dose schedules in girls 9-14 years to three-dose schedules in young women 15-26 years. we evaluate comparability of antibody levels between and within age groups and discuss potential implica ...	2015	25709084
ccctc-binding factor recruitment to the early region of the human papillomavirus 18 genome regulates viral oncogene expression.	host cell differentiation-dependent regulation of human papillomavirus (hpv) gene expression is required for productive infection. the host cell ccctc-binding factor (ctcf) functions in genome-wide chromatin organization and gene regulation. we have identified a conserved ctcf binding site in the e2 open reading frame of high-risk hpv types. using organotypic raft cultures of primary human keratinocytes containing high-risk hpv18 genomes, we show that ctcf recruitment to this conserved site regu ...	2015	25694598
mir-34a inhibits viability and invasion of human papillomavirus-positive cervical cancer cells by targeting e2f3 and regulating survivin.	previous studies confirmed that high-risk human papillomavirus (hr-hpv) infection is a risk factor of cervical cancer, and the infection was associated with significantly reduced mir-34a expression during carcinogenesis. however, the downstream targets of mir-34a and their roles are still not well understood. this study explored the regulative role of mir-34a on e2f3 and survivin expression and the viability and invasion of hpv-positive cervical cancer cells.	2015	25675046
zombies in tcga.	next-generation sequencing results obtained to detect somatic mutations in human cancers can also be searched for viruses that contribute to cancer. recently, human papillomavirus 18 rna was detected in tumor types not typically associated with hpv infection. analyses reported in this issue of journal of virology demonstrate that the apparent presence of hpv18 rna in these atypical tumors is due in at least some cases to contamination of samples with hela cells, which harbor hpv18.	2015	25631081
circulating human papillomavirus dna detected using droplet digital pcr in the serum of patients diagnosed with early stage human papillomavirus-associated invasive carcinoma.	specific human papillomavirus genotypes are associated with most ano-genital carcinomas and a large subset of oro-pharyngeal carcinomas. human papillomavirus dna is thus a tumour marker that can be detected in the blood of patients for clinical monitoring. however, data concerning circulating human papillomavirus dna in cervical cancer patients has provided little clinical value, due to insufficient sensitivity of the assays used for the detection of small sized tumours. here we took advantage o ...	2016	27917295
brd4 activates early viral transcription upon human papillomavirus 18 infection of primary keratinocytes.	human papillomaviruses (hpvs) replicate in the cutaneous and mucosal epithelia, and the infectious cycle is synchronous with the differentiation program of the host keratinocytes. the virus initially infects dividing cells in the lower layers of the epithelium, where it establishes a persistent infection. the viral genome is maintained as a low-copy-number, extrachromosomal element in these proliferating cells but switches to the late stage of the life cycle in differentiated cells. the cellular ...	2016	27879331
serine/arginine-rich splicing factor 3 and heterogeneous nuclear ribonucleoprotein a1 regulate alternative rna splicing and gene expression of human papillomavirus 18 through two functionally distinguishable cis elements.	human papillomavirus 18 (hpv18) is the second most common oncogenic hpv type associated with cervical, anogenital, and oropharyngeal cancers. like other oncogenic hpvs, hpv18 encodes two major (one early and one late) polycistronic pre-mrnas that are regulated by alternative rna splicing to produce a repertoire of viral transcripts for the expression of individual viral genes. however, rna cis-regulatory elements and trans-acting factors contributing to hpv18 alternative rna splicing remain unkn ...	2016	27489271
an exploration of individual- and population-level impact of the 2-dose hpv vaccination schedule in pre-adolescent girls.	since 2014, several countries have implemented a 2-dose schedule for human papillomavirus (hpv) vaccination. licensure of the 2-dose schedule was based on non-inferiority results from immunobridging studies, comparing the antibody levels of the 2-dose schedule in young girls to those of the 3-dose schedule in young adults. since licensure, additional data on antibody levels and other aspects of the immune response and clinical effectiveness have become available. this review will discuss the cur ...	2016	27171128
a highly sensitive quantum dots-dna nanobiosensor based on fluorescence resonance energy transfer for rapid detection of nanomolar amounts of human papillomavirus 18.	a very sensitive and convenient nanobiosensor based on fluorescence resonance energy transfer (fret) was developed for the detection of a 22-mer oligonucleotides sequence in human papillomavirus 18 virus (hpv18) gene. for this purpose, water-soluble cdte quantum dots (qds) were synthesized and, subsequently, amino-modified 11-mer oligonucleotide as one of the two necessary probes was attached to qds surface to form functional qds-dna conjugates. right after addition of the qds-dna and a second c ...	2017	28081500