Publications

calcium uptake by bacillus stearothermophilus: a requirement for thermophilic growth.		1976	1261679
characteristics of bacillus stearothermophilus mutants blocked in catabolic function.	with polyacrylamide disc gel electrophoresis and specific staining, it was demonstrated that one mutation involving the alcohol dehydrogenase of a double mutant of bacillus stearothermophilus 1503 apparently prevented enzyme synthesis, and another lesion in the same organism resulted in synthesis of an inactive form of aconitase. some properties of the double mutant and two fumarase mutants are discussed in relation to similar mutants derived from bacillus subtilis.	1976	1262307
thermal resistance of bacillus thermophilus spores suspended in parenteral solutions.		1976	1268431
surface topography of the bacillus stearothermophilus ribosome.	the surface topography of the intact 70s ribosomes and free 30s and 50s subunits from bacillus stearothermophilus strain 2184 was investigated by lactoperoxidase-catalyzed iodination. two-dimensional polyacrylamide gel electrophoresis was employed to separate ribosomal proteins for analysis of their reactivity. free 50s subunits incorporated about 18% more 125i than did 50s subunits derived from 70s ribosomes, whereas, free 30s subunits and 30s subunits derived from 70s ribosomes incorporated si ...	1976	1272247
association of ribosomal subunits: studies on the binding of polyamines to 30s and 50s particles.		1976	1278439
a comparison of the 16s ribosomal rnas from mesophilic and thermophilic bacilli: some modifications in the sanger method for rna sequencing.	two modifications in the sanger two dimensional electrophoretic procedure for rna analysis are reported. one increases resolution on the primary fingerprint to the point that digests of large rnas, of the size 1500-3000 nucleotides yield well resolved fingerprint patterns. the other is a novel endonucleolytic procedure that proves useful in determining sequences of the large oligonucleotides produced by t1 ribonuclease. these modifications have been used in determining the catalogs of oligomers ...	1976	819656
[the physiology of thermophilic and mesophilic bacilli during development at optimal and submaximal temperatures].	thermophilic bacilli, contrary to mesophilic, grow equally well under aerobic and anaerobic conditions. this may be due to the presence of more active anaerobic dehydrogenases in thermophilic organisms. the activity of glucose-6-phosphate dehydrogenase and alcohol dehydrogenase, and the content of atp, increased in the cells of thermophilic bac. stearothermophilus 159 and mesophilic bac. megaterium m strains growing at temperatures close to maximal. the activity of glutamate dehydrogenase under ...	1976	819761
protein synthesis at 680 atm: is it related to environmental origin, physiological type, or taxonomic group?	the ability of bacteria to synthesize protein at 680-atm pressure is not related to their environmental origin, physiological type, or taxonomic group.	1976	820255
thermophilic glyceraldehyde-3-p dehydrogenase.		1976	820563
role of calcium ion in the thermostability of alpha-amylase produced from bacillus stearothermophilus.		1976	820570
[drug residues in poultry (author's transl)].	the following microbiological tests: the bacillus subtilis bga (bundes-gesundheitsamt) test and sarcina lutea test as well as the test bacteria bacillus stearothermophilus var. calidolactis and e. coli were used to examine whether drug residues were detectable in broiler chickens during and after treatment with various drugs. with the exception of the occidiostat esb3, residues were not found to be present using the two above tests. on the other hand, residues of a number of drugs were detected ...	1976	821173
evaluation of a new design of bottle closure for non-injectable water.		1976	821906
[regulation of citrate synthese in bacteria: comparison of the action of various effectors on the enzymes of rhodospirillum rurbum and bacillus stearothermophilus].	a comparative study of the citrate synthases purified from the facultatively photosynthetic bacterium rhodospirillum rubrum (gram negative) and the thermophile bacillus stearothermophilus (gram positive) was made. the citrate synthase from r. rubrum was activated by kcl (6-fold at 0.1 m kcl) and, less effectively, by nacl and nh4cl. its molecular weight was about 300,000. the enzyme was strongly inhibited by nadh, and this inhibition was counteracted by amp. the citrate synthase from b. stearoth ...	1976	826987
the binding site of escherichia coli ribosomal protein s4 on 16-s ribosomal rna from different bacterial species.	ribosomal protein s4 of escherichia coli was bound to 16-s ribosomal rnas from several bacterial species and the complexes digested with pancreatic ribonuclease in an effort to isolate heterologous rna binding sites for protein s4. 16-s rnas from aeromonas punctata, pseudomonas fluorescens and vibrio cuneatus each gave rise to protected fragments whose electrophoretic mobility was 7s, i.e. similar to that of the fragment generated from e. coli 16-s rna using the same conditions. no comparable fr ...	1976	827436
thermophilic aminopeptidase i.		1976	827664
thermal denaturation of mesophilic and thermophilic 5s ribonucleic acids.	the tm of bacillus stearothermophilus 5s ribonucleic acid (rna) is 1.5 +/- 0.5 c higher than that of 5s rnas from b. subtilis and escherichia coli. melting in 50% methanol and in formaldehyde indicate that both base stacking and helical regions are involved in the slightly increased thermal stability of b. stearothermophilus 5s rna. it is probable that the 5s rna makes only a minor contribution to the thermostability of b. stearothermophilus 50s ribosomal subunits.	1976	815250
growth of bacilli on methyl-alpha-d-glucoside.		1976	815827
the primary structure of bacillus subtilis and bacillus stearothermophilus 5 s ribonucleic acids.	the nucleotide of bacillus stearothermophilus 5 s rna is pc-c-u-a-g-u-g-a-c-a-a-u-a-g-c-g-(g-a-g-a-g-g-)-a-a-a-c-a-c-c-c-g-u-u-c-c-c-a-u-c-c-c-g-a-a-c-a-c-g-g-a-a-g-u-u-a-a-g-c-u-c-u-c-c-a-g-c-g-c-c-g-a-u-g-g-u-a-g-u-u-g-g-g-g-c-c-a-g-c-g-c-c-c-c-u-g-c-a-a-g-a-g-u-a-g-g-u-c-g-u-u-g-c-u-a-g-g-coh; the nucelotide sequence of bacillus subtilis 5 s rna is pu-u-u-g-g-u-g-g-c-g-a-u-a-g-c-g-a-a-g-a-g-g-u-c-a-c-a-c-c-c-g-u-u-c-c-c-a-u-a-c-c-g-a-a-c-a-c-g-g-a-a-g-u-u-a-a-g-c-u-c-u-u-c-a-g-c-g-c-c-g-a-u-g ...	1976	818086
structural comparisons of mammalian, yeast and bacillar alcohol dehydrogenases.		1976	775100
thermal properties of glyceraldehyde 3-phosphate dehydrogenase from escherichia coli.	the molecular properties of glyceraldehyde 3-phosphate dehydrogenase from e. coli have been evaluated by circular dichroism and fluorescence emission spectroscopy measurements, with the purpose of studying the structural properties which are relevant for a comparison with the enzyme from the obligate thermophile bacillus stearothermophilus. the enzyme is moderately resistant to heat treatment, being pratically stable when treated for 10 min at 50 degrees c and completely inactivated when heating ...	1976	780122
comparative conformational properties of thermophilic and mesophilic 6-phosphogluconate dehydrogenase.	the structural properties of 6-phosphogluconate dehydrogenase from the mesophilic bacterium e. coli and the thermophilic b. stearothermophilus are compared using circular dichroism and fluorescence emission spectroscopy. the enzymes appear to possess a similar structure which does not change on heating up to the respective temperature of stability of the enzyme. the thermostability of the two 6-phosphogluconate dehydrogenases as determined by activity measurements parallels that determined by cd ...	1976	780123
isolation and some properties of enolase from bacillus stearothermophilus.		1976	780124
biochemical studies on an extreme thermophile thermus thermophilus: thermal stabilities of cell constituents and a bacteriophage.		1976	780125
purification and properties of two initiation factors from bacillus stearothermophilus.	two initiation factors have been isolated from the thermophilic bacterium, bacillus stearothermophilus, and purified to near homogeneity. the two factors possess physical characteristics and activities associated with the e. coli initiation factors if-2 and if-3, and are interchangeable with these factors. the two systems present, however, several differences : s-if-2 is significantly more heat stable than e. coli if-2, loosing less than 50 per cent of its activity after 20 minutes at 70degreesc ...	1976	782553
[the role of ribosomal proteins in translation in procaryota (author's transl)].		1976	785419
isolation and properties of 6-phosphogluconate dehydrogenase from escherichia coli. some comparisons with the thermophilic enzyme from bacillus stearothermophilus.	6-phosphogluconate dehydrogenase (6-phospho-d-gluconate:nadp oxidoreductase (decarboxylating), ec 1.1.1.44) of escherichia coli mrep 600 has been isolated with the purpose of carrying out comparative studies with the thermostable enzyme previously isolated from bacillus stearothermophilus (veronese, f.m., boccù, e.,fontana, a., benassi,c.a., and scoffone, e. (1974), biochim, biophys. acta 334, 31). the purified enzyme appeared homogeneous by the criteria of disc gel electrophoresis with and with ...	1976	786365
specific binding of formylated initiator-trna to escherichia coli rna polymerase.	e. coli fmet-trnafmeet and e. coli rna plymerase (rna nucleotidyltransferase; ec 2.7.7.6; nucleoside-triphosphate:rna nucleotidyltransferase) form a 1:1 complex with an apparent association constant of 9.0 x 10(6)m-1 at 37 degrees. the affinity of polymerase to trna depends on the trna as well as the formyl methionine moiety. core polymerase has a greatly reduced affinity for initiator trna. optimal binding conditions are similar to those that are also optimal for binding initiator trna to ribos ...	1976	787983
crystals of partially trypsin-digested elongation factor tu.		1976	789901
effect of temperature on the viability of bacillus stearothermophilus.	one of the obligate thermophilic bacteria, bacillus stearothermophilus, was unable to grow at temperatures below 35 degrees c. about 80% of the population in the bacterial culture died at the temperatures, and the same extent of loss in either of the activities of oxygen consumption or synthesis of protein or nucleic acid of the organisms was observed. with the progress of death of the organisms, reduced nicotinamide-adenine dinucleotide came to be oxidized by the organisms, enzymes such as fruc ...	1976	175753
reconstitution of bacillus stearothermophilus 50 s ribosomal subunits from purified molecular components.	bacillus stearothermophilus 50 s ribosomal subunits have been reconstituted from a mixture of purified rna and protein components. the protein fraction of 50 s subunits was separated into 27 components by a combination of various methods including ion exchange and gel filtration chromatography. the individual proteins showed single bands in a variety of polyacrylamide gel electrophoresis systems, and nearly all showed single spots on two-dimensional polyacrylamide gels. the molecular weights of ...	1976	172511
glyceraldehyde 3-phosphate dehydrogenase from an extreme thermophile, thermus aquaticus.		1976	181263
oxygen induced transformation of the nadh-nitrate oxidoreductase from bacillus stearothermophilus.		1976	181264
iodination of glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus.	the reaction of iodine with glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus was investigated. the active-site thiol group of the cysteine residue homologous with cysteine-149 in the pig muscle enzyme was protected by reaction with tetrathionate. the apoenzyme was readily inhibited by ki3 solution at ph8, but the coenzyme, nad+, protected the enzyme against inhibition and decreased the extent of iodination. at ph 9.5, ready inhibition of both apo- and holo-enzyme was obs ...	1976	182130
enzyme hyperspecificity. rejection of threonine by the valyl-trna synthetase by misacylation and hydrolytic editing.	valyl-trna synthetase from bacillus stearothermophilus activates thereonine and forms a 1:1 complex with threonyl adenylate, but it does not catalyze the net formation of threonyl-trnaval at ph 7.78 and 25 degrees c in the quenched flow apparatus it decomposes at a rate constant of 36s-1. during this process there is a transient formation of thr-trnaval reaching a maximum at 25 ms and rapidly falling to zero after 150 ms. at the peak, 22% of the (14c) threonine from the complex is present as ( ...	1976	182209
the relationship between environmental temperature, cell growth and the fluidity and physical state of the membrane lipids in bacillus stearothermophilus.	a definite and characteristic relationship exists between growth temperature, fatty acid composition and the fluidity and physical state of the membrane lipids in wild type bacillus stearothermophilus. as the environmental temperature is increased, the proportion of saturated fatty acids found in the membrane lipids is also markedly increased with a concomitant decrease in the proportion of unsaturated and branched chain fatty acids. the temperature range over which the gel to liquid-crystalline ...	1976	183821
phosphofructokinase from thermophilic micro-organisms.		1976	133030
succinylation of glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. a reactive threonine residue in the apoenzyme.	1. glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus can be extensively succinylated in the presence of substrates and coenzyme without appreciable loss of activity. 2. the apoenzyme in the absence of substrates is rapidly inhibited by small amounts of succinic anhydride. nad+, glyceraldehyde-3-phosphate and inorganic phosphate all afford protection from inhibition, and inhibition is slowly reversed in the presence of pyrophosphate at ph 8.5. 3. kinetic and spectral studi ...	1976	4305
glutamine synthetase of bacillus stearothermophilus. regulation, site interactions, and functional information.	the action of various feedback modifiers on bacillus stearothermophilus glutamine synthetase has been investigated by initial velocity kinetics, using the mn2+-stimulated biosynthetic assay at 55 degrees c. the most potent inhibitors, used singly, are amp, l-glutamine, and l-alanine. other modifiers of significance include glycine, ctp, l-histidine, glucosamine 6-phosphate, and gdp. marked synergism of action is observed for amp in the presence of l-glutamine, l-histidine, adp, or glucosamine 6- ...	1976	5112
[proceedings: structure of arginyl-t-rna synthetase sub-units from bacillus stearothermophilus].		1976	60939
proceedings: the primary structure of trna2val from bacillus stearothermophilus and its analogy with trnaval species from different origins.		1976	60956
beta-galactosidase from bacillus stearothermophilus.	several strains of thermophilic aerobic spore-forming bacilli synthesize beta-galactosidase (ec 3.2.1.23) constitutively. the constitutivity is apparently not the result of a temperature-sensitive repressor. the beta-galactosidase from one strain, investigated in cell-free extracts, has a ph optimum between 6.0 and 6.4 and a very sharp ph dependence on the acid side of its optimum. the optimum temperature for this enzyme is 65 degrees c and the arrhenius activation energy is about 24 kcal/mol be ...	1976	6142
maintainance of specificity, information, and thermostability in thermophilic bacillus sp. glutamine synthetase.	glutamine synthetase has been purified to homogeneity from b. subtilis (37 degrees) b. stearothermophilus (55 degrees), and b. caldolyticus (75 degrees). those characteristics compared include size (6.0 +/- 0.3 x 10(5) daltons), quaternary structure (12 su) amino acid content, substrate km's and specificity for structural analogs, metal ion activation, number and kind of separate feedback modifier sites, and the complexity of modifier-substrate and modifier-modifier site interactions. although t ...	1976	7467
some catalytic and molecular properties of threonine deaminase from bacillus stearothermophilus.	threonine deaminase ec 4.2.1.16 was highly purified from bacillus stearothermophilus. the enzyme exhibited maximum activity at 65 degrees and at ph 9.2--9.6. it was inactivated on dilution and on storage at 4 degrees, but was protected by egg albumin. the enzyme was labile at 65 degrees, but became stable in the presence of egg albumin and isoleucine at ph 7.0. the substrate saturation curve for the enzyme reaction at 40 or 65 degrees was hyperbolic, but in the presence of isoleucine, the curve ...	1976	10288
isolation and characterization of a bacteriocin produced by bacillus stearothermophilus strain nu-10.	broth-grown cultures of bacillus stearothermophilus strain nu-10 produce a bacteriocin which exerts lethal activity on other strains of the bacterium. optimal production occurs during late maximum stationary phase of growth, at neutral ph, and 55-65 degrees c. the bacteriocin can be substantially purified by a combination of precipitations, centrifugations, and gel filtrations. the thermocin is composed of protein and carbohydrate. it is partially destroyed by proteolytic enzymes but is resistan ...	1976	12863
internal sequence homology in e. coli isoleucyl- and valyl-trna synthetases.		1976	765150
isolation and properties of a thermostable restriction endonuclease (endo r-bst1503).	a restriction endonuclease was isolated from bacillus stearothermophilus1503-4r (bst1503) and purified to homogeneity. the enzyme required mg2+ ion as a cofactor. bst1503 exhibited maximal activity between ph 7.5 and 8.0, between 60 and 65 degrees c, and with about 0.2 mm mg2+. bst1503 was not inactivated after exposure at 55 or 65 degrees c for up to 10 h. after 2 h of incubation at 70 degrees c, bst1503 was inactivated by 65%. bst1503 was rapidly inactivated at 75 degrees c. a single protein-s ...	1977	14105
dtnb modification of sh groups of isocitrate dehydrogenase from bacillus stearothermophilus purified by affinity chromatography.	a new method of affinity chromatography using blue dextran-sepharose 4b resin was established to purify nadp+-dependent isocitrate dehydrogenase ec 1.1.1.42 from bacillus stearothermophilus in high yield. the purified preparation was found to be homogeneous on disc gel electrophoresis. the sh groups of the enzyme were modified with 5,5'-dithiobis-(2-nitrobenzoic acid) (dtnb) to determine the number of sh groups per molecule and their contribution to the enzyme activity. one sh group was titrated ...	1977	14937
a pulse-radiolysis study of the manganese-containing superoxide dismutase from bacillus stearothermophilus.	in the preceding paper the mechanism of catalysis of the manganese-containing superoxide dismutase from bacillus stearothermophilus was shown to involve a 'fast cycle' and a 'slow cycle' mcadam, fox, lavelle & fielden, 1977 (biochem. j. 165, 71-79). further properties of the enzyme was considered in the present paper. pulse-radiolysis studies, under conditions of low substrate concentration to (i.e. when the fast cycle predominates), showed that enzyme activity decreases as ph increases (6.5-10. ...	1977	19013
fluorescent labelling of 6-phosphogluconate dehydrogenase from bacillus stearothermophilus.	the thermophilic 6-phosphogluconate dehydrogenase from bacillus stearothermophilus was inhibited upon specific modification of the -sh group of cysteine residues by 7-chloro-4-nitrobenzo-2-oxa-1, 3-diazole (nbd-cl) at ph 7.0. by using 20-100-fold molar excess of nbd-cl the reaction occurs slowly at ph 7.0 as a first order process. partial protection from inactivation was observed when the substrate 6-phosphogluconate or the coenzyme nadp was added to the reaction mixture. complete inactivation w ...	1977	19368
affinity labeling of adenine nucleotide-related enzymes with reactive adenine nucleotide analogs. ii. affinity labeling of phosphoglycerate kinase with a reactive amp analog.	affinity labeling of yeast and b. stearothermophilus phosphoglycerate kinases with a reactive amp analog, n6-(p-bromoacetaminobenzyl)-amp was examined. complete loss of enzyme activity was observed when 1 mol of the reagent had reacted per mol of either enzyme. results on the effect of ph and substrate addition on the inactivation, titration of sh groups before and after modification, and kinetic studies with amp analogs suggest that the modification occurs at one amino group at or near the subs ...	1977	19433
the identification of lipid acceptor and the biosynthesis of lipid-linked glucose in bacillus stearothermophilus.		1977	20047
heat stable inhibitors produced in raw milk.	twenty raw farm milk samples were tested for inhibitory substances before and after being kept at room temperature until the ph fell to less than 6.3 but greater than 6.2. the tests were done using the idf-method and the commercial thermocult method. in both methods b. stearothermophilus var. chalidolactis is used as the test organism. in two instances a thermostable inhibitor was produced with an inhibitory potential greater than 0.01 iu/ml penicillin standard. the idf-method was found unsuitab ...	1977	21382
arginyl-trna synthetase from bacillus stearothermorphilus: characterization of the enzyme in presence of a protease inhibitor [proceedings].		1977	68732
effect of adp on atpase from a strain of bacillus stearothermophilus.	bacillus stearothermophilus atcc 12016 was unable to grow at temperatures below 40 degrees c. on incubating the bacteria at the temperatures, atp in cells disappeared, adp was accumulated and atpase (ec 3.6.1.3) was inactivated. when the purified atpase was incubated at the temperatures for 1 h with 0.17 mm adp in the presence of mgcl2, the enzyme was completely inactivated. the inactivated enzyme was reactivated on dilution or dialysis or on warming at 65 degrees c. during the incubation of the ...	1977	137750
escherichia coli 5s rna binding proteins l18 and l25 interact with 5.8s rna but not with 5s rna from yeast ribosomes.	reconstitution experiments showed that the two escherichia coli 5s rna binding proteins l18 and l25 form a specific complex with yeast 5.8s rna and not with yeast 5s rna. the yeast 5.8s rna-e. coli protein complex was found to exhibit atpase and gtpase activities that had previously been observed for the e. coli 5s rna-protein complex. the tetranucleotide upupcpg, which is an analog of the trna fragment tpsipcpg, interacted strongly with 5s rna-protein complexes from e. coli and bacillus stearot ...	1977	142985
phosphofructokinase from bacillus stearothermophilus [proceedings].		1977	143385
sequence and structure of d-glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus.	the glyceraldehyde 3-phosphate dehydogenase holoenzyme of bacillus stearothermophilus possesses precise 222 symmetry: in this respect it differs from the reported structure of the lobster muscle enzyme. pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer.	1977	193030
coenzyme binding and co-operativity in d-glyceraldehyde 3-phosphate dehydrogenase.		1977	198263
ligand binding stoichiometries, subunit structure, and slow transitions in aminoacyl-trna synthetases.		1977	199234
[genetics and biochemistry of the bacterial ribosome].		1977	322729
structural requirements of the gdp binding site of elongation factor tu.		1977	323049
the n-terminal sequence of superoxide dismutase from the strict anaerobe desulfovibrio desulfuricans.		1977	323056
the rates of evolution in some ribosomal components.	the rate of nucleotide substitution (k(nuc)) of 5s rna was estimated to be (1.8 +/- 0.5) x 10(-10) per site per year by comparing the nucleotide sequences of human and xenopus 5s rna and using the geological time elapsed since the separation of mammals and amphibians. similarly, k(nuc) of 5.8s rrna was calculated to be 0.93 10(-1u) per site per year from the sequences of rat hepatoma cells and saccbaromyces cerevisiae. for the comparison of these data with the amino acid substitution rate of kn ...	1977	325217
3' terminal sequences of 16s rrna do not explain translational specificity differences between e. coli and b. stearothermophilus ribosomes.		1977	327330
ribosomal protein s1/s1a in bacteria.		1977	330231
energy expenditure in the editing mechanism of protein synthesis [proceedings].		1977	332559
sequence homologies between the tryptophanyl trna synthetases of bacillus stearothermophilus and escherichia coli.		1977	334569
[studies on the structure and function of proteins--with special reference to glyceraldehyde 3-phosphate dehydrogenase (author's transl)].		1977	336812
[structure and function of ef-tu and ef-ts (author's transl)].		1977	336813
processing urinary endoscopes in a low-temperature steam and formaldehyde autoclave.	methods of disinfection and sterilisation of urinary endoscopes are considered. a small mobile low-temperature steam and formaldehyde autoclave (miniclave 80) is evaluated and shown to be satisfactory for this purpose as judged by a variety of relevant microbiological test pieces.	1977	557503
inhibition of fructose-1,6-bisphosphate aldolase from rabbit muscle and bacillus stearothermophilus.	phosphoglycollohydroxamic acid and phosphoglycollamide are inhibitors of rabbit muscle fructose-1,6-bisphosphate aldolase. the binding dissociation constants determined by enzyme inhibition and protein fluorescence quenching suggest that two distinct enzyme inhibitor complexes may be formed. the binding dissociation constants of the two inhibitors to bacillus stearothermophilus cobalt (ii) fructose-1,6-bisphosphate aldolase have also been determined. the hydroxamic acid is an exceptionally poten ...	1977	562749
[3-phosphoglycerate kinase from microorganisms (author's transl)].		1977	347503
a revised sequence for bacillus stearothermophilus phenylalanine trna.		1977	598504
method for the immobilization of bacterial spores in alginate gel.		1977	599891
letter to the editor.		1977	604365
kind and frequency of toxin production by cereals contaminating aspergilli and penicillia.		1977	613911
[determination of residual antibiotic activity in cerebro-spinal fluid (author's transl)].	a microbiological method for the determination of residual antibiotic activity in csf is described. this disc-diffusion method, utilizing bacillus stearotermophilus as test organism, is highly sensitive for many antibiotics, including those crossing the blood-brain barrier.	1977	616243
[the demonstration of antibacteric power in a blood sample taken for blood culture. preliminary note. (author's transl)].	to identify a possible "false" negative blood culture from a patient not declearing antibiotic treatment, we propose a "residual antibiotic power" test (par-test) on the same blood sample to be rum in parallel with the blood culture. hence we propose using b. stearothermophilus var. calidolactis, which we have found very sensitive to the common antibiotics used in human medicine. suggested technique: agar+germ with an absorbent paper disc soaked with the sample, incubated for 3 hours at 60 degre ...	1977	616251
kinetic evidence for an acyl-enzyme intermediate in d-alanine carboxypeptidases of bacillus subtilis and bacillus stearothermophilus.	the kinetics of hydrolysis and transpeptidation of the synthetic substrate diacetyl-l-lysyl-d-alanyl-d-alanine and of the natural substrate udp-acetylmuramyl pentapeptide and related compounds catalyzed by the d-alanine carboxypeptidases of bacillus subtilis and bacillus stearothermophilus in the presence of the nucleophiles hydroxylamine or glycine have been examined. these kinetic data suggest that an acyl-enzyme intermediate is formed in the first step of the reaction and that the transpeptid ...	1977	404297
gardimycin, a new antibiotic inhibiting peptidoglycan synthesis.	gardimycin, a new antibiotic, at 100 mug/ml, specifically inhibited cell wall synthesis and induced accumulation of uridine 5'-diphosphate-n-acetylmur-amylpentapeptide in whole cells of bacillus subtilis. the antibiotic was active in a particulate enzyme preparation from bacillus stearothermophilus: 60 mug/ml caused 50%, and 200mug/ml caused 100%, inhibition of peptidoglycan synthesis. suppression of peptidoglycan synthesis was accompanied by parallel accumulation of the lipid intermediate. this ...	1977	404960
[biological indicators: research on the resistance ratio between b. stearothermophilus and bacillus subtilis and pumilis].		1977	405247
microwave oven irradiation as a method for bacterial decontamination in a clinical microbiology laboratory.	exposure of 10 frequently isolated clinical pathogens to microwave irradiation resulted in total sterilization with 60 s. time exposure experiments done with commercially prepared test strips containing bacillus stearothermophilus spores indicated that 5-min exposure was adequate to insure sterility of small, contaminated loads.	1977	410828
inactivation of bacillus spores in dry systems at low and high temperatures.	a plot of the thermal resistance of bacillus subtilis var. niger spores (log d value) against temperature was linear between 37 and 190 degrees c (z = 23 degrees c), provided that the relative humidity of the spore environment was kept below a certain critical level. the corresponding plot for bacillus stearothermophilus spores was linear in the range 150 to 180 degrees c (z = 29 degrees c) but departed from linearity at lower temperatures (decreasing z value). however, the z value of 29 degrees ...	1977	411885
[methods of demonstrating antibiotics residues in meat, organs and eggs].		1977	416557
purification and properties of homoprotocatechuate 2,3-dioxygenase from bacillus stearothermophilus.	the enzyme 3,4-dihydroxyphenylacetate:oxygen 2,3-oxidoreductase (decyclizing) (homoprotocatechuate 2,3-dioxygenase) was purified from the thermophilic organism bacillus stearothermophilus, grown with j-hydroxyphenylacetic acid as a source of carbon. the enzyme appeared to be homogeneous as judged by disc-gel electrophoresis and sedimentation equilibrium measurements. the average molecular weight determined by three independent procedures was 106,000; the protein was globular and was dissociated ...	1977	838683
the use of alpha-galactosidase and invertase in hollow fiber reactors.	invertase and alpha-galactosidase have been immobilized in hollow fiber cartridges with no detectable enzyme leakage and used for the hydrolysis of sucrose and raffinose, respectively. for both hollow fiber immobilized enzymes nearly complete substrate conversion is possible. enzyme stabilities in polysulfonate hollow fibers which have been preconditioned with bovine albumin approach the stabilities of the free enzymes.	1977	843613
dna polymerase from mesophilic and thermophilic bacteria. iii. lack of fidelity in the replication of synthetic polydeoxyribonucleotides by dna polymerase from bacillus licheniformis and bacillus stearothermophilus.	1. dna polymerase from the mesophile bacillus licheniformis and the thermophile bacillus stearothermophilus has been used to study the replication of poly(da-dt)-poly(da-dt) and poly(dc)-poly(dg) templates at 37, 45, and 55 degrees c. 2. incorporation of non-complementary deoxyribonucleoside triphosphates (misincorporation) occurred with both enzymes and both templates. non-specific incorporation (de novo polynucleotide synthesis, random attachment to existing strands, and tritium exchange of nu ...	1977	849445
folding domains and intramolecular ionic interactions of lysine residues in glyceraldehyde 3-phosphate dehydrogenase.	1. treatment with methyl acetimidate was used to probe the topography of several tetrameric glyceraldehyde 3-phosphate dehydrogenases, in particular the holoenzymes from rabbit muscle and bacillus stearothermophilus. during the course of the reaction with the rabbit muscle enzyme, the number of amino groups fell rapidly from the starting value of 27 per subunit to a value of approx. five per subunit. this number could be lowered further to values between one and two per subunit by a second treat ...	1977	851424
thermophilic aminopeptidase. iv. cooperative effects in ans binding by the thermophilic aminopeptidase i from b. stearothermophilus.	aminopeptidase i is a membrane-bound metalloenzyme isolated from b. stearothermophilus which is thermostable and requires co2+ for activity. the co, zn, and metal-free enzyme were titrated with ans, and cooperative binding was noted with the active co enzyme but not with the zn (5% active) or apo (inactive) enzymes. there are a number of sites for ans on each of the three enzyme forms and the agreement between the association constants of the zn enzyme (identical and independent sites) and the n ...	1977	856749
preliminary x-ray diffraction studies of orthorhombic crystals of superoxide dismutase from bacillus stearothermophilus.		1977	875027
[a microbiological method for the rapid determination of antibiotic concentrations during therapy (author's transl)].	the assay described is an agar diffusion test using bacillus stearothermophilus as test organism. incubation is at 62 degrees c; results can be obtained rapidly within 2 to 3 hours. the test was developed for the determination of gentamicin concentrations in serum and other body fluids. because of its high sensitivity the test strain is also suitable for the determination of other antibiotics. the test is designed primarily for the determination of concentrations in individual samples and thus f ...	1977	881260
a pulse-radiolysis study of the manganese-containing superoxide dismutase from bacillus stearothermophilus. a kinetic model for the enzyme action.	the enzymic reaction mechanism of a manganese-containing superoxide dismutase from bacillus stearothermophilus was studied by using pulse radiolysis. during catalysis (ph 8.9; 25 degrees c), changes occurring in the kinetics of substrate disappearance and in the visible absorption of the enzyme at 480 nm established that the simple two-step mechanism found for copper- and iron-containing superoxide dismutases is not involved. at a low ratio (less than 15) of substrate concentration to enzyme con ...	1977	889577
the binding site of ribosomal protein s8 on the 16-s ribosomal rna from bacillus stearothermophilus.		1977	891539
temperature-induced metabolic alterations in a thermophilic bacillus.		1977	891545
the amino acid sequence of tryptophanyl trna synthetase from bacillus stearothermophilus.		1977	891985
detection of residual penicillins in milk by using a bacillus stearothermophilus disk assay.	a paper disk method based on a procedure described by the international dairy federation is presented for the detection of 4 beta-lactam antibiotics (penicillin g, ampicillin, cephapirin, and cloxacillin) in whole milk. bacillus stearothermophilus var. calidolactis, prepared as a stable spore suspension, was used as the test organism. levels of 0.005 unit penicillin g/ml, 0.005 microgram ampicillin or cephapirin/ml, and 0.05 microgram cloxacillin/ml were readily detected in whole milk. results w ...	1977	893331
superoxide dismutase from bacillus stearothermophilus: reversible removal of manganese and its replacement by other metals [proceedings].		1977	923975
new rapid gel sequencing method for rna.		1977	927512
the binding site of ribosomal protein l1 from escherichia coli on the 23-s ribosomal rna from bacillus stearothermophilus. a possible base-pairing scheme differing from that proposed for escherichia coli.	the region of bacillus stearothermophilus strain nca 1503 23-s ribosomal rna protected from t1 ribonuclease digestion by the 50-s ribosomal subunit protein l1 from escherichia coli has been established. the sequence of 115 nucleotides is compared to the analogous region in e. coli. the similar behaviour of the rna towards the recognition of protein l1 may be explained in terms of secondary base-pairing, even though there exists almost 40% difference between the primary nucleotide sequences.	1978	416958
further evidence for the structure of the teichoic acids from bacillus stearothermophilus b65 and bacillus subtilis var. niger wm.	bacillus stearothermophilus b65 and bacillus subtilis var. niger wm both contain teichoic acids in their walls composed of glycerol, phosphate and glucose. the 13c nuclear magnetic resonance spectrum of b. stearothermophilus teichoic acid showed 13c-31p coupling on the signals from the c-5 and c-6 carbon atoms of the glucose molecule and an alpha-glucosidic linkage between glucose and the c-1 atom of the glycerol moiety. these data are consistent with a poly[glucosylglycerol phosphate] as the ce ...	1978	417919