Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| wolbachia infection density in populations of the asian citrus psyllid (hemiptera: liviidae). | the symbiotic relationships between bacteria of the genus wolbachia (order rickettsiales) and their arthropod hosts are diverse and can range from mutualism to parasitism. whereas effects of wolbachia on host biology are well investigated, little is known about diversity and abundance of wolbachia in their natural hosts. the phloem-feeding asian citrus psyllid, diaphorina citri (kuwayama) (hemiptera: liviidae), is naturally infected with wolbachia (wdi). in the current study, we calculated the w ... | 2014 | 25259690 |
| population dynamics and growth rates of endosymbionts during diaphorina citri (hemiptera, liviidae) ontogeny. | the infection density of symbionts is among the major parameters to understand their biological effects in host-endosymbionts interactions. diaphorina citri harbors two bacteriome-associated bacterial endosymbionts (candidatus carsonella ruddii and candidatus profftella armatura), besides the intracellular reproductive parasite wolbachia. in this study, the density dynamics of the three endosymbionts associated with the psyllid d. citri was investigated by real-time quantitative pcr (qpcr) at di ... | 2014 | 25037159 |
| insecticide sprays, natural enemy assemblages and predation on asian citrus psyllid, diaphorina citri (hemiptera: psyllidae). | the asian citrus psyllid (acp), diaphorina citri kuwayama is considered a key citrus pest due to its role as vector of 'huanglongbing' (hlb) or citrus greening, probably the most economically damaging disease of citrus. insecticidal control of the vector is still considered a cornerstone of hlb management to prevent infection and to reduce reinoculation of infected trees. the severity of hlb has driven implementation of intensive insecticide programs against acp with unknown side effects on bene ... | 2014 | 24830653 |
| biology of the huanglongbing vector diaphorina citri (hemiptera: liviidae) on different host plants. | although many studies have been conducted on the development and reproductive potential of diaphorina citri kuwayama, 1908 (hemiptera: liviidae) in different host species, few have evaluated these parameters on different varieties of the same host species. this study evaluated the influence of five commercial varieties of citrus (citrus spp. l.)--hamlin, natal, pêra, ponkan, and valencia-and orange jasmine [murraya exotica (l.) jack] on the development of d. citri. survival rates for the egg sta ... | 2014 | 24772551 |
| nested-quantitative pcr approach with improved sensitivity for the detection of low titer levels of candidatus liberibacter asiaticus in the asian citrus psyllid, diaphorina citri kuwayama. | candidatus liberibacter asiaticus (clas) is a phloem-limited bacterium transmitted by the asian citrus psyllid, diaphorina citri, and the presumptive causal agent of citrus greening disease. the current method of detection for clas within plant and insect samples is by a presence/absence qpcr assay using the clas 16s rdna gene target. although qpcr is highly sensitive, low bacterial titers or suboptimal qpcr conditions can result in false-negatives. using a nested qpcr assay, we determined the f ... | 2014 | 24769405 |
| host range testing of tamarixia radiata (hymenoptera: eulophidae) sourced from the punjab of pakistan for classical biological control of diaphorina citri (hemiptera: liviidae: euphyllurinae: diaphorinini) in california. | abstract tests evaluating the host range of tamarixia radiata (waterson) (hymenoptera: eulophidae), a parasitoid of the pestiferous asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), sourced from the punjab of pakistan, were conducted in quarantine at the university of california, riverside, ca. seven nontarget psyllid species (five native and two self-introduced species) representing five families were exposed to t radiata under the following three different exposure scenari ... | 2014 | 24665694 |
| characterization of the asian citrus psyllid transcriptome. | the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae) is a vector for the causative agents of huanglongbing, which threatens citrus production worldwide. this study reports and discusses the first d. citri transcriptomes, encompassing the three main life stages of d. citri, egg, nymph and adult. the transcriptomes were annotated using gene ontology (go) and insecticide-related genes within each life stage were identified to aid the development of future d. citri insecticides ... | 2014 | 24511328 |
| the phloem-sap feeding mealybug (ferrisia virgata) carries 'candidatus liberibacter asiaticus' populations that do not cause disease in host plants. | 'candidatus liberibacter asiaticus' (las) is the primary causal agent of huanglongbing (hlb), the most devastating disease of citrus worldwide. there are three known insect vectors of the hlb-associated bacteria, and all are members of the hemiptera: diaphorina citri (psyllidae), trioza erytreae (triozidae), and cacopsylla (psylla) citrisuga (psyllidae). in this study, we found that another hemipteran, the striped mealybug ferrisia virgata (cockerell) (hemiptera: pseudococcidae), was able to acq ... | 2014 | 24465578 |
| innate and conditioned responses to chemosensory and visual cues in asian citrus psyllid, diaphorina citri (hemiptera: liviidae), vector of huanglongbing pathogens. | asian citrus psyllid (diaphorina citri) transmits huanglongbing, a devastating disease that threatens citrus trees worldwide. a better understanding of the psyllid's host-plant selection process may lead to the development of more efficient means of monitoring it and predicting its movements. since behavioral adaptations, such as associative learning, may facilitate recognition of suitable host-plants, we examined whether adult d. citri could be conditioned to visual and chemosensory stimuli fro ... | 2014 | 26462949 |
| semiochemical and vibrational cues and signals mediating mate finding and courtship in psylloidea (hemiptera): a synthesis. | mate finding and courtship involve complex interactions that require close coordination between individuals of the opposite gender. well-organized signalling systems, sometimes involving a combination of signal modalities, are required to convey species-specific and individual information to members of the opposite gender. previous studies of psyllids have focused on single-signal modalities and have largely ignored the potentially interdependent nature of different types of signals. several stu ... | 2014 | 26462826 |
| the push-pull strategy for citrus psyllid control. | the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: psyllidae), is the only natural vector of candidatus liberibacter asiaticus that causes citrus huanglongbing (hlb), a most destructive disease of citrus. currently, no remedial therapy exists for the disease, and so effective control of acp is very important in curbing the transmission of the disease. the push-pull strategy should be thoroughly explored as an approach to acp management. this mini-review summarises the current ... | 2015 | 25256398 |
| comprehensive meta-analysis, co-expression, and mirna nested network analysis identifies gene candidates in citrus against huanglongbing disease. | huanglongbing (hlb), the most devastating disease of citrus, is associated with infection by candidatus liberibacter asiaticus (calas) and is vectored by the asian citrus psyllid (acp). recently, the molecular basis of citrus-hlb interactions has been examined using transcriptome analyses, and these analyses have identified many probe sets and pathways modulated by calas infection among different citrus cultivars. however, lack of consistency among reported findings indicates that an integrative ... | 2015 | 26215595 |
| cloning and expressing a highly functional and substrate specific farnesoic acid o-methyltransferase from the asian citrus psyllid (diaphorina citri kuwayama). | the asian citrus psyllid, diaphorina citri, transmits a phloem-limited bacterium, candidatus 'liberibacter' asiaticus that causes citrus greening disease. because juvenile hormone (jh) plays an important role in adult and nymphal development, we studied the final steps in jh biosynthesis in d. citri. a putative jh acid methyltransferase ortholog gene (jmtd) and its cognate cdna were identified by searching d. citri genome database. expression analysis shows expression in all life stages. in adul ... | 2015 | 25893162 |
| asian citrus psyllid expression profiles suggest candidatus liberibacter asiaticus-mediated alteration of adult nutrition and metabolism, and of nymphal development and immunity. | the asian citrus psyllid (acp) diaphorina citri kuwayama (hemiptera: psyllidae) is the insect vector of the fastidious bacterium candidatus liberibacter asiaticus (clas), the causal agent of citrus greening disease, or huanglongbing (hlb). the widespread invasiveness of the psyllid vector and hlb in citrus trees worldwide has underscored the need for non-traditional approaches to manage the disease. one tenable solution is through the deployment of rna interference technology to silence protein- ... | 2015 | 26091106 |
| morphological, molecular and virulence characterization of three lencanicillium species infecting asian citrus psyllids in huangyan citrus groves. | citrus greening or huanglongbing (hlb) is caused by the infection of candidatus liberibacter spp. in citrus plants. since asian citrus psyllid is the primary vector of this bacterial pathogen, the spread of hlb can be mitigated by suppressing asian citrus psyllid populations in citrus groves using entomopathogens. to expand the current data on entomopathogens infecting asian citrus psyllids, we isolated and characterized three different entomopathogens. strains zjlsp07, zjla08, and zjlp09 infect ... | 2015 | 25593036 |
| chemical control of the asian citrus psyllid and of huanglongbing disease in citrus. | by 2014, huanglongbing (hlb), the most destructive disease of citrus, and its insect vector, the asian citrus psyllid (acp), diaphorina citri (kuwayama), became established in all major citrus-growing regions of the world, including the united states, with the exception of california. at present, application of insecticides is the most widely followed option for reducing acp populations, while application of antibiotics for suppressing hlb disease/symptoms is being practiced in some citrus-growi ... | 2015 | 25491482 |
| lethal and sublethal impacts of acaricides on tamarixia radiata (hemiptera: eulophidae), an important ectoparasitoid of diaphorina citri (hemiptera: liviidae). | the use of synthetic acaricides for management of pest mites may alter the efficacy of the ectoparasitoid tamarixia radiata (waterston) in biological control of diaphorina citri kuwayama, the vector of the bacteria associated with huanglongbing (hlb) in citrus orchards. we evaluated the toxicity of 16 acaricides that are recommended for the control of citrus-pest mites to t. radiata. acrinathrin, bifenthrin, carbosulfan, and fenpropathrin caused high acute toxicity and were considered harmful (m ... | 2015 | 26453716 |
| changes in variable number of tandem repeats in 'candidatus liberibacter asiaticus' through insect transmission. | citrus greening (huanglongbing) is the most destructive citrus disease worldwide. the disease is associated with three species of 'candidatus liberibacter' among which 'ca. liberibacter asiaticus' has the widest distribution. 'ca. l. asiaticus' is commonly transmitted by a phloem-feeding insect vector, the asian citrus psyllid diaphorina citri. a previous study showed that isolates of 'ca. l. asiaticus' were clearly differentiated by variable number of tandem repeat (vntr) profiles at four loci ... | 2015 | 26402645 |
| metabolic interplay between the asian citrus psyllid and its profftella symbiont: an achilles' heel of the citrus greening insect vector. | 'candidatus liberibacter asiaticus' (clas), the bacterial pathogen associated with citrus greening disease, is transmitted by diaphorina citri, the asian citrus psyllid. interactions among d. citri and its microbial endosymbionts, including 'candidatus profftella armatura', are likely to impact transmission of clas. we used quantitative mass spectrometry to compare the proteomes of clas(+) and clas(-) populations of d. citri, and found that proteins involved in polyketide biosynthesis by the end ... | 2015 | 26580079 |
| draft genome sequence of "candidatus liberibacter asiaticus" from diaphorina citri in guangdong, china. | the draft genome sequence of "candidatus liberibacter asiaticus" strain ycpsy from an asian citrus psyllid (diaphorina citri) in guangdong, china, is reported here. the ycpsy strain has a genome size of 1,233,647 bp, 36.5% g+c content, 1,171 open reading frames (orfs), and 53 rnas. | 2015 | 26543132 |
| whole-genome sequence of "candidatus profftella armatura" from diaphorina citri in guangdong, china. | the genome of "candidatus profftella armatura" strain ycpa from diaphorina citri in guangdong, china, was sequenced. the strain has a chromosome of 457,565 bp, 24.3% g+c content, 364 predicted open reading frames (orfs), and 38 rnas, and a plasmid, pycpa54, of 5,458 bp with 23.9% g+c content and 5 orfs. | 2015 | 26543117 |
| sampling methods for detection and monitoring of the asian citrus psyllid (hemiptera: psyllidae). | the asian citrus psyllid (acp), diaphorina citri kuwayama is a key pest of citrus due to its role as vector of citrus greening disease or "huanglongbing." acp monitoring is considered an indispensable tool for management of vector and disease. in the present study, datasets collected between 2009 and 2013 from 245 citrus blocks were used to evaluate precision, sensitivity for detection, and efficiency of five sampling methods. the number of samples needed to reach a 0.25 standard error-mean rati ... | 2015 | 26313984 |
| lethal and sublethal effects of insecticides used on citrus, on the ectoparasitoid tamarixia radiata. | huanglongbing (hlb) is a disease associated with the bacteria "candidatus liberibacter spp." and has been devastating citrus orchards around the world. its management involves control of the insect vector, the asian citrus psyllid diaphorina citri kuwayama. however, the indiscriminate use of chemicals has caused pest outbreaks and eliminated the natural enemies of the vector, such as the parasitoid tamarixia radiata (waterston), the main agent for biological control of d. citri. this study asses ... | 2015 | 26132327 |
| morphometric comparisons of diaphorina citri (hemiptera: liviidae) populations from iran, usa and pakistan. | the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: liviidae), vector of citrus greening disease pathogen, huanglongbing (hlb), is considered the most serious pest of citrus in the world. prior molecular based studies have hypothesized a link between the d. citri in iran and the usa (florida). the purpose of this study was to collect morphometric data from d. citri populations from iran (mtcoi haplotype-1), florida (mtcoi haplotype-1), and pakistan (mtcoi haplotype-6), to deter ... | 2015 | 26038715 |
| asymptomatic spread of huanglongbing and implications for disease control. | huanglongbing (hlb) is a bacterial infection of citrus trees transmitted by the asian citrus psyllid diaphorina citri. mitigation of hlb has focused on spraying of insecticides to reduce the psyllid population and removal of trees when they first show symptoms of the disease. these interventions have been only marginally effective, because symptoms of hlb do not appear on leaves for months to years after initial infection. limited knowledge about disease spread during the asymptomatic phase is e ... | 2015 | 26034273 |
| imidacloprid sorption kinetics, equilibria, and degradation in sandy soils of florida. | imidacloprid (imd) is a neonicotinoid insecticide soil-drenched on sandy soils of southwest florida for the control of diaphorina citri kuwayama or asian citrus psyllid (acp). the acp vectors causal pathogens of a devastating citrus disease called citrus greening. understanding the behavior of imd in these soils and plants is critical to its performance against target pests. samples from immokalee fine sand (ifs) were used for sorption kinetics and equilibria experiments. imd kinetics data were ... | 2015 | 25938475 |
| infection density dynamics of the citrus greening bacterium "candidatus liberibacter asiaticus" in field populations of the psyllid diaphorina citri and its relevance to the efficiency of pathogen transmission to citrus plants. | huanglongbing, or citrus greening, is a devastating disease of citrus plants recently spreading worldwide, which is caused by an uncultivable bacterial pathogen, "candidatus liberibacter asiaticus," and vectored by a phloem-sucking insect, diaphorina citri. we investigated the infection density dynamics of "ca. liberibacter asiaticus" in field populations of d. citri with experiments using field-collected insects to address how "ca. liberibacter asiaticus" infection density in the vector insect ... | 2015 | 25819961 |
| internal extracellular bacteria of diaphorina citri kuwayama (hemiptera: psyllidae), the asian citrus psyllid. | the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae), is an invasive insect pest that transmits candidatus liberibacter spp. this insect/pathogen system was first identified in north america in the early 2000's and has become the top threat to the citrus industry. limited options for management of this problem exist; therefore, innovative pest management strategies are being developed. in this study, we describe the first step toward a paratransgenic approach (also referred ... | 2015 | 25645736 |
| effects of methoprene, a juvenile hormone analog, on survival of various developmental stages, adult emergence, reproduction and behavior of asian citrus psyllid, diaphorina citri kuwayama. | the asian citrus citrus psyllid, diaphorina citri kuwayama, transmits a bacterium that causes huanglongbing in citrus. frequent and repeated use of neurotoxic insecticides against d. citri has resulted in the development of insecticide resistance. we evaluated the effects of the juvenile hormone analog methoprene on egg hatch, nymphal development, adult emergence, reproduction and behavior of d. citri. | 2015 | 25583143 |
| characterization of a recombinant cathepsin b-like cysteine peptidase from diaphorina citri kuwayama (hemiptera: liviidae): a putative target for control of citrus huanglongbing. | huanglonbing (hlb) is one of the most destructive disease affecting citrus plants. the causal agent is associated with the phloem-limited bacterium candidatus liberibacter asiaticus (clas) and the psyllid diaphorina citri, vector of disease, that transmits the bacterium associated with hlb. the control of disease can be achieved by suppressing either the bacterium or the vector. among the control strategies for hlb disease, one of the widely used consists in controlling the enzymes of the diseas ... | 2015 | 26717484 |
| diverse array of new viral sequences identified in worldwide populations of the asian citrus psyllid (diaphorina citri) using viral metagenomics. | the asian citrus psyllid, diaphorina citri, is the natural vector of the causal agent of huanglongbing (hlb), or citrus greening disease. together; hlb and d. citri represent a major threat to world citrus production. as there is no cure for hlb, insect vector management is considered one strategy to help control the disease, and d. citri viruses might be useful. in this study, we used a metagenomic approach to analyze viral sequences associated with the global population of d. citri. by sequenc ... | 2015 | 26676774 |
| bionomics of asian citrus psyllid (hemiptera: liviidae) associated with orange jasmine hedges in southeast central florida, with special reference to biological control by tamarixia radiata. | the asian citrus psyllid, diaphorina citri kuwayama, is an important pest in florida because it transmits bacteria responsible for citrus huanglongbing disease. in addition to infesting citrus, orange jasmine (murraya exotica l.) is one of asian citrus psyllid's preferred host plants and is widely grown as an ornamental hedge. we report on asian citrus psyllid bionomics over three years at five urban plantings of orange jasmine and on biological control of asian citrus psyllid by a parasitoid ta ... | 2015 | 26470246 |
| host range testing of diaphorencyrtus aligarhensis (hymenoptera: encyrtidae) for use in classical biological control of diaphorina citri (hemiptera: liviidae) in california. | host range tests for diaphorencyrtus aligarhensis (shafee, alam, & agarwal) (hymenoptera: encyrtidae), an endoparasitoid of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), sourced from punjab pakistan, were conducted in quarantine at the university of california, riverside, ca. seven nontarget psyllid species representing four psyllid families were exposed to mated d. aligarhensis females in four different treatment types: 1) short sequential no-choice treatments, 2) prolo ... | 2015 | 26470214 |
| diaphorina citri (hemiptera: liviidae) vector competence for the citrus greening pathogen 'candidatus liberibacter asiaticus'. | characterizing the vector competence of diaphorina citri kuwayama for 'candidatus liberibacter asiaticus,' the pathogen causing citrus greening, is essential for understanding the epidemiology of this disease that is threatening the u.s. citrus industry. vector competence studies have been difficult because of the biology of d. citri, the inability to culture the pathogen, and the available diagnostic methods used to detect the bacteria in plant and insect tissues. the methods employed in many s ... | 2015 | 26470202 |
| effect of cyantraniliprole, a novel insecticide, on the inoculation of candidatus liberibacter asiaticus associated with citrus huanglongbing by the asian citrus psyllid (hemiptera: liviidae). | the asian citrus psyllid (diaphorina citri kuwayama) is the principal vector of 'candidatus liberibacter asiaticus' (clas) associated with huanglongbing (hlb), the most serious citrus disease worldwide. new control measures including pesticides are urgently needed to combat hlb, especially to protect young or newly planted citrus trees from clas-inoculation by vector psyllids. here, we tested clas-inoculation by d. citri adults (clas-exposed, reared on infected plants) by feeding them for 7 d on ... | 2015 | 26470150 |
| diaphorina citri (hemiptera: liviidae) abundance in puerto rico declines with elevation. | diaphorina citri kuwayama is the primary vector of huanglongbing, the most devastating disease of citrus. d. citri populations in puerto rico were monitored with yellow sticky traps on citrus trees or other psyllid host plants at different elevations, ranging from 10 to 880 m above sea level. trapping was conducted in march through may of 2013 and 2014 when psyllid populations usually are highest. population levels of d. citri, based on the trapping data, varied among the sites, and there was a ... | 2015 | 26470127 |
| effect of uv-blocking plastic films on take-off and host plant finding ability of diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is a major pest of citrus worldwide due to its ability to transmit the bacteria associated with huanglongbing. vision, behavior, and performance of insect pests can be manipulated by using ultraviolet (uv)-blocking materials. thus, the aim of our study was to evaluate how uv-blocking plastic films may affect the take-off and host plant finding ability of d. citri. to assess the effect of a uv-deficient environment on take ... | 2015 | 26470126 |
| seasonal movement patterns and long-range dispersal of asian citrus psyllid in florida citrus. | the asian citrus psyllid, diaphorina citri kuwayama, is the vector of the bacterial pathogen, candidatus liberibacter asiaticus, which is the causal agent of huanglongbing (hlb) in the united states. both short-range and long-range dispersal of d. citri adults affect the spread of hlb; however, little is known about the long-range dispersal capabilities of d. citri in the field or the seasonality of flight behavior. in the present study, an in situ protein marking technique was used to determine ... | 2015 | 26470097 |
| infection of an insect vector with a bacterial plant pathogen increases its propensity for dispersal. | the spread of vector-transmitted pathogens relies on complex interactions between host, vector and pathogen. in sessile plant pathosystems, the spread of a pathogen highly depends on the movement and mobility of the vector. however, questions remain as to whether and how pathogen-induced vector manipulations may affect the spread of a plant pathogen. here we report for the first time that infection with a bacterial plant pathogen increases the probability of vector dispersal, and that such movem ... | 2015 | 26083763 |
| living on the edges: spatial niche occupation of asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), in citrus groves. | the spatial niche occupation of the asian citrus psyllid, diaphorina citri kuwayama, 1908, was evaluated to determine its field colonization and food resource exploitation strategies in citrus groves. mature grapefruit and sweet orange groves were surveyed as part of an area-wide program in 2009-2010 to determine d. citri population densities and between-tree distribution. in both cultivars, significantly more psyllids were found on perimeter trees throughout the study period suggesting a strong ... | 2015 | 26193111 |
| transmission rates of ‘ca. liberibacter asiaticus’ by asian citrus psyllid are enhanced by the presence and developmental stage of citrus flush. | asian citrus psyllid (diaphorina citri kuwayama) transmits a bacterium ‘candidatus liberibacter asiaticus’ (clas) putatively responsible for a devastating citrus disease known as asiatic huanglongbing (hlb) (citrus greening disease). the psyllid and disease have invaded many citrus-growing regions including the united states, where the disease is seriously jeopardizing the florida citrus industry. we recently concluded research that showed clas transmission rates are increased when citrus flush ... | 2016 | 26884596 |
| thiamethoxam and imidacloprid drench applications on sweet orange nursery trees disrupt the feeding and settling behaviour of diaphorina citri (hemiptera: liviidae). | chemical control is the method most used for management of diaphorina citri, the vector of the phloem-limited bacteria associated with citrus huanglongbing (hlb) disease. the objectives of this study were to determine the influence of soil-drench applications of neonicotinoids (thiamethoxam and imidacloprid) on the probing behaviour of d. citri on citrus nursery trees, using the electrical penetration graph (epg) technique, and to measure the d. citri settling behaviour after probing on citrus n ... | 2016 | 26694803 |
| diaphorina citri induces huanglongbing-infected citrus plant volatiles to repel and reduce the performance of propylaea japonica. | transmission of plant pathogens through insect vectors is a complex biological process involving interactions between the host plants, insects, and pathogens. simultaneous impact of the insect damage and pathogenic bacteria in infected host plants induce volatiles that modify not only the behavior of its insect vector but also of their natural enemies, such as parasitoid wasps. therefore, it is essential to understand how insects such as the predator ladybird beetle responds to volatiles emitted ... | 2016 | 28083006 |
| disrupting mating behavior of diaphorina citri (liviidae). | severe economic damage from citrus greening disease, caused by 'candidatus liberibacter asiaticus' bacteria, has stimulated development of methods to reduce mating and reproduction in populations of its insect vector, diaphorina citri (hemiptera: liviidae). male d. citri find mating partners by walking on host plants, intermittently producing vibrational calls that stimulate duetting replies by receptive females. the replies provide orientational feedback, assisting the search process. to test a ... | 2016 | 27707945 |
| effects of candidatus liberibacter asiaticus on the fitness of the vector diaphorina citri. | the asian citrus psyllid (acp), diaphorina citri kuwayama transmits the bacterium 'candidatus liberibacter asiaticus' (las), which causes citrus huanglongbing (hlb) disease. although many studies have been conducted on the biology of acp on different host plants, few have taken the plant, las bacteria and the vector insect within one context to evaluate the effects of las on the fitness of acp under field conditions. understanding the relationship between las and acp is critical for both acp and ... | 2016 | 27667799 |
| two-spotted ladybeetle adalia bipunctata l. (coleoptera: coccinellidae): a commercially available predator to control asian citrus psyllid diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid diaphorina citri kuwayama (hemiptera: liviidae) is an economically important pest of citrus because it serves as a vector of the causal pathogens of huanglongbing (hlb) also known as citrus greening disease. the increased use of insecticides for control of d. citri negatively impacts several natural enemies including some effective ladybeetle species which are not available commercially. the two-spotted ladybeetle, adalia bipunctata (coleoptera: coccinellidae) is found i ... | 2016 | 27631730 |
| complete genome sequence of the largest known flavi-like virus, diaphorina citri flavi-like virus, a novel virus of the asian citrus psyllid, diaphorina citri. | a novel flavi-like virus tentatively named diaphorina citri flavi-like virus (dcflv) was identified in field populations of diaphorina citri through small rna and transcriptome sequencing followed by reverse transcription (rt)-pcr. we report here the complete nucleotide sequence and genome organization of dcflv, the largest flavi-like virus identified to date. | 2016 | 27609921 |
| a review of techniques for detecting huanglongbing (greening) in citrus. | huanglongbing (hlb) is the most destructive disease of citrus worldwide. monitoring of health and detection of diseases in trees is critical for sustainable agriculture. hlb symptoms are virtually the same wherever the disease occurs. the disease is caused by candidatus liberibacter spp., vectored by the psyllids diaphorina citri kuwayama and trioza erytreae. electron microscopy was the first technique used for hlb detection. nowadays, scientists are working on the development of new techniques ... | 2016 | 27590666 |
| a phagostimulant blend for the asian citrus psyllid. | chemical cues that elicit orientation by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), are of interest because it is the primary vector of the causal pathogen of citrus greening disease. non-pesticidal control methods for d. citri remain a high priority for the citrus industry. while searching for semiochemicals that may be involved in orientation to host plants, we previously identified a blend of formic and acetic acids that stimulated substrate probing by d. citri ... | 2016 | 27542089 |
| complete genome sequence of a putative densovirus of the asian citrus psyllid, diaphorina citri. | here, we report the complete genome sequence of a putative densovirus of the asian citrus psyllid, diaphorina citri diaphorina citri densovirus (dcdnv) was originally identified through metagenomics, and here, we obtained the complete nucleotide sequence using pcr-based approaches. phylogenetic analysis places dcdnv between viruses of the ambidensovirus and iteradensovirus genera. | 2016 | 27469948 |
| complete genome sequence of diaphorina citri-associated c virus, a novel putative rna virus of the asian citrus psyllid, diaphorina citri. | we present here the complete nucleotide sequence and genome organization of a novel putative rna virus identified in field populations of the asian citrus psyllid, diaphorina citri, through sequencing of the transcriptome followed by reverse transcription-pcr (rt-pcr). we tentatively named this virus diaphorina citri-associated c virus (dcacv). dcacv is an unclassified positive-sense rna virus. | 2016 | 27445370 |
| better together: association with 'candidatus liberibacter asiaticus' increases the reproductive fitness of its insect vector, diaphorina citri (hemiptera: liviidae). | the duration of the evolutionary association between a pathogen and vector can be inferred based on the strength of their mutualistic interactions. a well-adapted pathogen is likely to confer some benefit or, at a minimum, exhibit low pathogenicity toward its host vector. coevolution of the two toward a mutually beneficial association appears to have occurred between the citrus greening disease pathogen, candidatus liberibacter asiaticus (las), and its insect vector, the asian citrus psyllid, di ... | 2016 | 27418697 |
| essential oil variation from twenty two genotypes of citrus in brazil-chemometric approach and repellency against diaphorina citri kuwayama. | the chemical composition of volatile oils from 22 genotypes of citrus and related genera was poorly differentiated, but chemometric techniques have clarified the relationships between the 22 genotypes, and allowed us to understand their resistance to d. citri. the most convincing similarities include the synthesis of (z)-β-ocimene and (e)-caryophyllene for all 11 genotypes of group a. genotypes of group b are not uniformly characterized by essential oil compounds. when stimulated with odor sourc ... | 2016 | 27338332 |
| exposure to guava affects citrus olfactory cues and attractiveness to diaphorina citri (hemiptera: psyllidae). | intercropping can reduce agricultural pest incidence, and represents an important sustainable alternative to conventional pest control methods. understanding the ecological mechanisms for intercropping could help optimize its use, particularly in tropical systems which present a large number of intercropping possibilities. citrus is threatened worldwide by greening disease (huanglongbing, hlb) vectored by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: psyllidae). control of hlb ... | 2016 | 27247354 |
| disruption of vector host preference with plant volatiles may reduce spread of insect-transmitted plant pathogens. | plant pathogens can manipulate the odor of their host; the odor of an infected plant is often attractive to the plant pathogen vector. it has been suggested that this odor-mediated manipulation attracts vectors and may contribute to spread of disease; however, this requires further broad demonstration among vector-pathogen systems. in addition, disruption of this indirect chemical communication between the pathogen and the vector has not been attempted. we present a model that demonstrates how a ... | 2016 | 27193763 |
| imidacloprid extraction from citrus leaves and analysis by liquid chromatography-mass spectrometry (hplc-ms/ms). | a procedure was developed to extract imidacloprid (imd) from newly-flushed and fully-expanded citrus leaves. the extraction was conducted in a bullet blender, using a small sample mass (0.5 g of fresh tissue), stainless-steel beads (24 g), and methanol as extractant (10 ml). the extracts did not require further clean-up before analysis by hplc-ms/ms. the method was validated with control samples from imd-untreated hamlin orange trees. the method limit of detection and limit of quantitation were ... | 2016 | 26988226 |
| phenology of asian citrus psyllid (hemiptera: liviidae), with special reference to biological control by tamarixia radiata, in the residential landscape of southern california. | since its discovery in 2008, the pestiferous asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), has become widely established in residential citrus trees throughout southern california. in 2011, tamarixia radiata (waterston) (hymenoptera: eulophidae), a host-specific parasitoid of d. citri, sourced from punjab pakistan, was introduced into california as part of a classical biological program aimed at suppressing d. citri populations in urban areas. despite these release effor ... | 2016 | 26957552 |
| the influence of learning on host plant preference in a significant phytopathogen vector, diaphorina citri. | although specialist herbivorous insects are guided by innate responses to host plant cues, host plant preference may be influenced by experience and is not dictated by instinct alone. the effect of learning on host plant preference was examined in the asian citrus psyllid, diaphorina citri; vector of the causal agent of citrus greening disease or huanglongbing. we investigated: a) whether development on specific host plant species influenced host plant preference in mature d. citri; and b) the e ... | 2016 | 26930355 |
| formic and acetic acids in degradation products of plant volatiles elicit olfactory and behavioral responses from an insect vector. | volatile phytochemicals play a role in orientation by phytophagous insects. we studied antennal and behavioral responses of the asian citrus psyllid, diaphorina citri, vector of the citrus greening disease pathogen. little or no response to citrus leaf volatiles was detected by electroantennography. glass cartridges prepared with β-ocimene or citral produced no response initially but became stimulatory after several days. both compounds degraded completely in air to a number of smaller molecules ... | 2016 | 26857741 |
| inter-population variability of endosymbiont densities in the asian citrus psyllid (diaphorina citri kuwayama). | the asian citrus psyllid (diaphorina citri kuwayama) is an insect pest capable of transmitting candidatus liberibacter asiaticus (clas), the causal agent of citrus greening in north america. d. citri also harbors three endosymbionts, wolbachia, candidatus carsonella ruddii, and candidatus profftella armatura, which may influence d. citri physiology and fitness. although genomic researches on these bacteria have been conducted, much remains unclear regarding their ecology and inter-population var ... | 2016 | 26846216 |
| transmission and propagation of 'candidatus liberibacter asiaticus' by grafting with individual citrus leaves. | huanglongbing (hlb) is a chronic, progressive decline disease in citrus associated with a systemic infection by the bacterium 'candidatus liberibacter asiaticus'. transmission of the bacterium in the field is by the asian citrus psyllid, diaphorina citri kuwayama. experimental propagation of 'ca. l. asiaticus' is done primarily by grafting pieces of bud wood from an infected plant. to produce a small-scale model system for investigation of pathogen biology, we investigated grafting single leaves ... | 2016 | 26807818 |
| huanglongbing: an overview of a complex pathosystem ravaging the world's citrus. | citrus huanglongbing (hlb) has become a major disease and limiting factor of production in citrus areas that have become infected. the destruction to the affected citrus industries has resulted in a tremendous increase to support research that in return has resulted in significant information on both applied and basic knowledge concerning this important disease to the global citrus industry. recent research indicates the relationship between citrus and the causal agent of hlb is shaped by multip ... | 2016 | 26466921 |
| impact of different temperatures on survival and energy metabolism in the asian citrus psyllid, diaphorina citri kuwayama. | temperature influences the life history and metabolic parameters of insects. asian citrus psyllid (acp), diaphorina citri is a tropical and subtropical pest. acp invaded new regions around the world and threatened the citrus industry as a vector for huanglongbing (hlb) disease. acp is widely distributed and can survive high (up to 45 °c) and low temperatures (as low as -6 °c). the precise mechanism of temperature tolerance in acp is poorly understood. we investigated adult survival, cellular ene ... | 2016 | 26603556 |
| monitoring for insecticide resistance in asian citrus psyllid (hemiptera: psyllidae) populations in florida. | the development of insecticide resistance in asian citrus psyllid, diaphorina citri kuwayama, populations is a serious threat to the citrus industry. as a contribution to a resistance management strategy, we developed a glass vial technique to monitor field populations of asian citrus psyllid for insecticide resistance. diagnostic concentrations needed to separate susceptible genotypes from resistant individuals were determined for cypermethrin (0.5 μg per vial), malathion (1.0 μg per vial), dia ... | 2016 | 26709293 |
| quality matters: influences of citrus flush physicochemical characteristics on population dynamics of the asian citrus psyllid (hemiptera: liviidae). | studies were conducted to relate the influence of the physical characteristics, leaf nutrient content and phloem sap amino acid concentration of citrus flush shoots on the densities of various diaphorina citri life stages. adult d. citri preferentially selected young shoots for feeding and numbers of d. citri immatures were positively correlated with flush shoot softness. young flush shoots had higher concentrations of macro and micro nutrients relative to mature ones and this was associated wit ... | 2016 | 28030637 |
| efficacy of essential oil of piper aduncum against nymphs and adults of diaphorina citri. | insecticide application is the main way to control diaphorina citri. however, it causes environmental contamination, has a negative impact on beneficial organisms and leads to psyllid resistance. the essential oil of piper aduncum has low toxicity towards the environment and contains dillapiol, which has proven to be effective against several crop pests. here, we studied its efficacy against nymphs and adults of d. citri under laboratory conditions. oils with three concentrations of dillapiol (6 ... | 2016 | 26331551 |
| evolutionary conservation of candidate osmoregulation genes in plant phloem sap-feeding insects. | the high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. the genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid acyrthosiphon pisum: sucrase 1 (suc1), a sucrase in glycoside hydrolase family 13 (gh13), and aquaporin 1 (aqp1), a member of the drosophila integral protein (drip) family of aquaporins. here, we describe molecular anal ... | 2016 | 26896054 |
| nucleotides, micro- and macro-nutrients, limonoids, flavonoids, and hydroxycinnamates composition in the phloem sap of sweet orange. | currently, the global citrus production is declining due to the spread of huanglongbing (hlb). hlb, otherwise known as citrus greening, is caused by candidatus liberibacter asiaticus (clas) and is transmitted by the asian citrus psyllids (acp), diaphorina citri kuwayama. acp transmits clas bacterium while feeding on the citrus phloem sap. multiplication of clas in the phloem of citrus indicates that the sap contains all the essential nutrients needed for clas. in this study, we investigated the ... | 2016 | 27171979 |
| laser system for identification, tracking, and control of flying insects. | flying insects are common vectors for transmission of pathogens and inflict significant harm to humans and agricultural production in many parts of the world. we present proof of principle for an optical system capable of highly specific vector control. this system utilizes a combination of optical sources, detectors, and sophisticated software to search, detect, and identify flying insects in real-time, with the capability of eradication using a lethal laser pulse. we present data on two insect ... | 2016 | 27410106 |
| digital gene expression analysis of ponkan mandarin (citrus reticulata blanco) in response to asia citrus psyllid-vectored huanglongbing infection. | citrus huanglongbing (hlb), the most destructive citrus disease, can be transmitted by psyllids and diseased budwoods. although the final symptoms of the two main hlb transmission ways were similar and hard to distinguish, the host responses might be different. in this study, the global gene changes in leaves of ponkan (citrus reticulata) mandarin trees following psyllid-transmission of hlb were analyzed at the early symptomatic stage (13 weeks post inoculation, wpi) and late symptomatic stage ( ... | 2016 | 27384559 |
| metabolomic comparative analysis of the phloem sap of curry leaf tree (bergera koenegii), orange jasmine (murraya paniculata), and valencia sweet orange (citrus sinensis) supports their differential responses to huanglongbing. | orange jasmine, murraya paniculata and curry leaf tree, bergera koenegii are alternative hosts for diaphorina citri, the vector of candidatus liberibacter asiaticus (clas), the pathogen of huanglongbing (hlb) in citrus. d. citri feeds on the phloem sap where clas grows. it has been shown that orange jasmine was a better host than curry leaf tree to d. citri. in addition, clas can infect orange jasmine but not curry leaf tree. here, we compared the phloem sap composition of these 2 plants to the ... | 2016 | 27763819 |
| enhanced acquisition rates of 'candidatus liberibacter asiaticus' by the asian citrus psyllid (hemiptera: liviidae) in the presence of vegetative flush growth in citrus. | the asian citrus psyllid preferentially feeds and exclusively reproduces on young, newly emerged flush shoots of citrus. asian citrus psyllid nymphs feed and complete their life stages on these flush shoots. recent studies conducted under greenhouse conditions have shown that the transmission rates of 'candidatus liberibacter asiaticus' (clas), the putative causal agent of huanglongbing disease of citrus, are enhanced when flush shoots are present. however, it is unclear if clas acquisition by m ... | 2016 | 27451998 |
| repellent activity of botanical oils against asian citrus psyllid, diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid, diaphorina citri kuwayama, is the insect vector of the pathogen causing huanglongbing. we selected three botanical oils to evaluate behavioral activity against d. citri. in laboratory olfactometer assays, fir oil was repellent to d. citri females, while litsea and citronella oils elicited no response from d. citri females. in choice settling experiments, d. citri settled almost completely on control plants rather than on plants treated with fir oil at a 9.5 mg/day relea ... | 2016 | 27429006 |
| acquisition, replication and inoculation of candidatus liberibacter asiaticus following various acquisition periods on huanglongbing-infected citrus by nymphs and adults of the asian citrus psyllid. | the asian citrus psyllid, diaphorina citri (hemiptera: liviidae), is the primary vector of candidatus liberibacter asiaticus (las) implicated as causative agent of citrus huanglongbing (citrus greening), currently the most serious citrus disease worldwide. las is transmitted by d. citri in a persistent-circulative manner, but the question of replication of this bacterium in its psyllid vector has not been resolved. thus, we studied the effects of the acquisition access period (aap) by nymphs and ... | 2016 | 27441694 |
| asian citrus psyllid rnai pathway - rnai evidence. | diaphorina citri, known as the asian citrus psyllid, is an important pest of citrus because it transmits a phloem-limited bacteria strongly implicated in huanglongbing (citrus greening disease). emerging biotechnologies, such as rna interference, could provide a new sustainable and environmentally friendly strategy for the management of this pest. in this study, genome and functional analysis were performed to verify whether the rnai core genes are present in the asian psyllid genome and if the ... | 2016 | 27901078 |
| invasive mutualisms between a plant pathogen and insect vectors in the middle east and brazil. | complex multi-trophic interactions in vectorborne diseases limit our understanding and ability to predict outbreaks. arthropod-vectored pathogens are especially problematic, with the potential for novel interspecific interactions during invasions. variations and novelties in plant-arthropod-pathogen triumvirates present significant threats to global food security. we examined aspects of a phytoplasma pathogen of citrus across two continents. 'candidatus phytoplasma aurantifolia' causes witches' ... | 2016 | 28083099 |
| morphological abnormalities and cell death in the asian citrus psyllid (diaphorina citri) midgut associated with candidatus liberibacter asiaticus. | candidatus liberibacter asiaticus (clas) is a phloem-limited, gram-negative, fastidious bacterium that is associated with the development of citrus greening disease, also known as huanglongbing (hlb). clas is transmitted by the asian citrus psyllid (acp) diaphorina citri, in a circulative manner. two major barriers to transmission within the insect are the midgut and the salivary glands. we performed a thorough microscopic analysis within the insect midgut following exposure to clas-infected cit ... | 2016 | 27630042 |
| host plant affects morphometric variation of diaphorina citri (hemiptera: liviidae). | the asian citrus psyllid (acp), diaphorina citri kuwayama, is one of the most serious citrus pests worldwide due to its role as vector of huanglongbing or citrus greening disease. while some optimal plant species for acp oviposition and development have been identified, little is known of the influence of host plants on acp size and shape. our goal was to determine how size and shape of acp wing and body size varies when development occurs on different host plants in a controlled rearing environ ... | 2016 | 27833820 |
| antennal and abdominal transcriptomes reveal chemosensory genes in the asian citrus psyllid, diaphorina citri. | the asian citrus psyllid, diaphorina citri is the principal vector of the highly destructive citrus disease called huanglongbing (hlb) or citrus greening, which is a major threat to citrus cultivation worldwide. more effective pest control strategies against this pest entail the identification of potential chemosensory proteins that could be used in the development of attractants or repellents. however, the molecular basis of olfaction in the asian citrus psyllid is not completely understood. th ... | 2016 | 27441376 |
| species clarification of isaria isolates used as biocontrol agents against diaphorina citri (hemiptera: liviidae) in mexico. | entomopathogenic fungi belonging to the genus isaria (hypocreales: cordycipitaceae) are promising candidates for microbial control of insect pests. currently, the mexican government is developing a biological control program based on extensive application of isaria isolates against diaphorina citri (hemiptera: liviidae), a vector of citrus huanglongbing disease. previous research identified three promising isaria isolates (che-cnrcb 303, 305, and 307; tentatively identified as isaria fumosorosea ... | 2016 | 26895870 |
| oviposition behavior and survival of tamarixia radiata (hymenoptera: eulophidae), an ectoparasitoid of the asian citrus psyllid, diaphorina citri (hemiptera: liviidae), on hosts exposed to an entomopathogenic fungus, isaria fumosorosea (hypocreales: cordycipitaceae), under laboratory conditions. | antagonistic interactions between the nymphal parasitoid, tamarixia radiata waterston (hymenoptera: eulophidae), and the arsef 3581 strain of the entomopathogenic fungus, isaria fumosorosea wize (hypocreales: cordycipitaceae), could disrupt biological control of the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). three interactions were evaluated under laboratory conditions at 25 °c: 1) parasitoid survival if parasitized hosts were exposed to arsef 3581 blastospores before ... | 2016 | 27435930 |
| annotation of the asian citrus psyllid genome reveals a reduced innate immune system. | citrus production worldwide is currently facing significant losses due to citrus greening disease, also known as huanglongbing. the citrus greening bacteria, candidatus liberibacter asiaticus (clas), is a persistent propagative pathogen transmitted by the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae). hemipterans characterized to date lack a number of insect immune genes, including those associated with the imd pathway targeting gram-negative bacteria. the d. citri draft ... | 2016 | 27965582 |
| functional characterization of lotp from liberibacter asiaticus. | liberibacter asiaticus is an unculturable parasitic bacterium of the alphaproteobacteria group hosted by both citrus plants and a psyllid insect vector (diaphorina citri). in the citrus tree, the bacteria thrive only inside the phloem, causing a systemically incurable and deadly plant disease named citrus greening or huanglongbing. currently, all commercial citrus cultivars in production are susceptible to l. asiaticus, representing a serious threat to the citrus industry worldwide. the technica ... | 2017 | 28378385 |
| costs and benefits of insecticide and foliar nutrient applications to huanglongbing-infected citrus trees. | the asian citrus psyllid (acp), diaphorina citri kuwayama (hemiptera: psyllidae), vectors candidatus liberibacter asiaticus, which causes huanglongbing (hlb). in florida, hlb incidence is approaching 100% statewide. yields have decreased and production costs have increased since 2005. despite this, some growers are maintaining a level of production and attribute this in part to aggressive psyllid control and foliar nutrition sprays. however, the value of these practices is debated. a replicated ... | 2017 | 27427170 |
| one target, two mechanisms: the impact of candidatus liberibacter asiaticus and its vector, diaphorina citri on citrus leaf pigments. | huanglongbing is currently the largest threat to global citrus production. we examined the effect of hlb pathogen, candidatus liberibacter asiaticus-infection and/or its vector, diaphorina citri-infestation on valencia sweet orange leaf pigments using hplc, followed by gene expression analysis for 46 involved genes in carotenoids- and chlorophylls-biosynthesis pathways. both clas and d. citri alter the total citrus leaf pigments balance with a greater impact by clas. while zeaxanthin was accumul ... | 2017 | 28358623 |
| risk assessment of various insecticides used for management of asian citrus psyllid, diaphorina citri in florida citrus, against honey bee, apis mellifera. | the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is a major pest of citrus trees worldwide. a wide variety of insecticides are used to manage d. citri populations within citrus groves in florida. however, in areas shared by citrus growers and beekeepers the use of insecticides may increase the risks of apis mellifera l. (hymenoptera: apidae) loss and contaminated honey. the objective of this research was to determine the environmental toxicity of insecticides, spanning ... | 2017 | 28116643 |
| characterization of the voltage-gated sodium channel of the asian citrus psyllid, diaphorina citri. | the asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is an important insect pest of citrus. it is the vector of 'candidatus' liberibacter asiaticus, a phloem-limited bacterium that infects citrus, resulting in the disease huanglongbing (hlb). disease management relies heavily on suppression of d. citri populations with insecticides, including pyrethroids. in recent annual surveys to monitor insecticide resistance, reduced susceptibility to fenpropathrin was identified in se ... | 2017 | 26537022 |
| comparative proteomic analysis of hemolymph from uninfected and candidatus liberibacter asiaticus-infected diaphorina citri. | hemolymph was characterized from diaphorina citri adults infected with the phytopathogen, candidatus liberibacter asiaticus (clas), and compared with that from uninfected psyllids. this study identified 5531 and 3220 peptides within infected and uninfected hemolymph using nano-lc-ms/ms. a reduced number of proteins were detected for d. citri and all known endosymbionts within infected hemolymph as compared to uninfected hemolymph. a large number of immune defense proteins were absent from d. cit ... | 2017 | 27990558 |
| latency and persistence of 'candidatus liberibacter asiaticus' in its psyllid vector, diaphorina citri (hemiptera: liviidae). | although 'candidatus liberibacter asiaticus' (las) is a major pathogen associated with citrus huanglongbing (hlb), some characteristics of transmission by the psyllid vector diaphorina citri are not fully understood. we examined the latent period and persistence of transmission of las by d. citri in a series of experiments at 25°c, in which third-instar psyllid nymphs and 1-week-old adults were confined on infected citrus for an acquisition access period (aap), and submitted to sequential inocul ... | 2017 | 27841960 |
| evaluation of systemic neonicotinoid insecticides for the management of the asian citrus psyllid diaphorina citri on containerized citrus. | studies were conducted to evaluate uptake and retention of three systemic neonicotinoid insecticides, dinotefuran, imidacloprid and thiamethoxam, in potted citrus nursery plants treated at standard label rates. infestation of these plants placed at a field site with moderate levels of asian citrus psyllid (acp) was monitored for 14 weeks following treatments, and insecticide residues in leaf tissue were quantified using enzyme-linked immunosorbent assay (elisa). bioassays were conducted using le ... | 2017 | 27714916 |
| a plant bacterial pathogen manipulates its insect vector's energy metabolism. | insect-transmitted plant-pathogenic bacteria may alter their vectors' fitness, survival, behavior, and metabolism. because these pathogens interact with their vectors on the cellular and organismal levels, potential changes at the biochemical level might occur. "candidatus liberibacter asiaticus" (clas) is transmitted in a persistent, circulative, and propagative manner. the genome of clas revealed the presence of an atp translocase that mediates the uptake of atp and other nucleotides from medi ... | 2017 | 28039132 |
| effects of temperature, photoperiod, and rainfall on morphometric variation of diaphorina citri (hemiptera: liviidae). | phenotypic plasticity provides a mechanism by which an organism can adapt to new or changing environments. earlier studies have demonstrated the variability of diaphorina citri kuwayama (asian citrus psyllid) population dynamics, but no analysis of morphological changes induced by seasonal or artificial laboratory-induced conditions has yet been documented. such morphometric variation has been found to correspond in dispersal capabilities in several insect taxa. in this study, the effects of tem ... | 2017 | 28031429 |
| protein interaction networks at the host-microbe interface in diaphorina citri, the insect vector of the citrus greening pathogen. | the asian citrus psyllid (diaphorina citri) is the insect vector responsible for the worldwide spread of 'candidatus liberibacter asiaticus' (clas), the bacterial pathogen associated with citrus greening disease. developmental changes in the insect vector impact pathogen transmission, such that d. citri transmission of clas is more efficient when bacteria are acquired by nymphs when compared with adults. we hypothesize that expression changes in the d. citri immune system and commensal microbiot ... | 2017 | 28386418 |
| genetic diversity of diaphorina citri and its endosymbionts across east and southeast asia. | diaphorina citri is the vector of 'candidatus liberibacter asiaticus', the most widespread pathogen associated huanglongbing, the most serious disease of citrus. to enhance our understanding of the distribution and origin of the psyllid, we investigated the genetic diversity and population structures of 24 populations in asia and one from florida based on the mtcoi gene. simultaneously, genetic diversity and population structures of the primary endosymbiont (p-endosymbiont) 'candidatus carsonell ... | 2017 | 28374537 |
| influence of abiotic factors on flight initiation by asian citrus psyllid (hemiptera: liviidae). | predicting the emergence and arrival of insect pests is paramount for integrated pest management. to achieve this goal, it is important to understand how abiotic factors influence pest dispersal behavior. we investigated the effects of abiotic conditions on flight initiation by the asian citrus psyllid, diaphorina citri kuwayama. we first explored the effect of barometric pressure changes on flight initiation. we used a custom-made barometric chamber and observed the activity of d. citri as meas ... | 2017 | 28334135 |
| oral delivery of double-stranded rnas induces mortality in nymphs and adults of the asian citrus psyllid, diaphorina citri. | the asian citrus psyllid (acp), diaphorina citri kuwayama, is one of the most important citrus pests. acp is the vector of the phloem-limited bacteria candidatus liberibacter americanus and candidatus liberibacter asiaticus, the causal agents of the devastating citrus disease huanglongbing (hlb). the management of hlb is based on the use of healthy young plants, eradication of infected plants and chemical control of the vector. rna interference (rnai) has proven to be a promising tool to control ... | 2017 | 28282380 |
| sclerenchymatous ring as a barrier to phloem feeding by asian citrus psyllid: evidence from electrical penetration graph and visualization of stylet pathways. | asian citrus psyllid (diaphorina citri) feeding behaviors play a significant role in the transmission of the phloem-limited candidatus liberibacter asiaticus (clas) bacterium that causes the economically devastating citrus greening disease. sustained phloem ingestion by d. citri on clas infected plants is required for pathogen acquisition and transmission. recent studies have shown a fibrous ring of thick-walled sclerenchyma around the phloem in mature, fully expanded citrus leaves that is more ... | 2017 | 28278248 |
| spatiotemporal dynamics of the southern california asian citrus psyllid (diaphorina citri) invasion. | biological invasions are governed by spatial processes that tend to be distributed in non-random ways across landscapes. characterizing the spatial and temporal heterogeneities of the introduction, establishment, and spread of non-native insect species is a key aspect of effectively managing their geographic expansion. the asian citrus psyllid (diaphorina citri), a vector of the bacterium associated with huanglongbing (hlb), poses a serious threat to commercial and residential citrus trees. in 2 ... | 2017 | 28278188 |
| rna interference of carboxyesterases causes nymph mortality in the asian citrus psyllid, diaphorina citri. | asian citrus psyllid, diaphorina citri kuwayama (hemiptera: liviidae), is an important pest of citrus. in addition, d. citri is the vector of huanglongbing, a destructive disease in citrus, also known as citrus greening disease caused by candidatus liberibacter asiaticus. huanglongbing causes huge losses for citrus industries. insecticide application for d. citri is the major strategy to prevent disease spread. the heavy use of insecticides causes development of insecticide resistance. we used r ... | 2017 | 28195667 |
| double-stranded rna delivery through soaking mediates silencing of the muscle protein 20 and increases mortality to the asian citrus psyllid, diaphorina citri. | asian citrus psyllid, diaphorina citri kuwayama, is the most important economic pest of citrus because it transmits candidatus liberibacter asiaticus (clas), the causal agent of huanglongbing (hlb). silencing genes by rna interference (rnai) is a promising approach for controlling d. citri. rnai-based insect management strategies depend on the selection of suitable target genes. | 2017 | 28195429 |