Publications

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the influence of cadmium on life-history parameters and gut microflora of archegozetes longisetosus (acari: oribatida) under laboratory conditions.we tested the effect of cadmium (25, 130 microg cd g(-1)), administered via chinese cabbage (brassica chinensis l.) as food on life-history parameters and gut microflora of tritonymphs and adults of the oribatid mite, archegozetes longisetosus aoki. both concentrations of cd had an adverse effect on offspring mortality, and the higher concentration also reduced female fecundity, as well as the number of bacteria, fungi and actinomycetes, and it changed the community structure of bacteria; the pr ...200918979170
molecular cloning and characterization of a novel pollen predominantly membrane protein gene bcmf12 from brassica campestris ssp. chinensis.a novel membrane protein gene, bcmf12, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino) using rapid amplification of the cdna ends based on a pollen-specific cdna fragment (dn237936). the cdna was 1,155 bp in length with an open reading frame of 894 bp capable of encoding a putative polypeptide of 297 amino acids with an estimated molecular mass of 34.6 kda and a predicted isoelectric point of 9.6. comparative and bioinformatics analyses revealed that bcmf12 showe ...200919169847
[transpiration of brassica chinensis l. in a plastic greenhouse covered with insect-proof nets in lower reaches of yangtze river: a simulation study].with the climate data inside and outside a plastic greenhouse as driving variables, and the greenhouse structure, insect-proof net material, and characteristic breadth and leaf area index of brassica chinensis l. as parameters; a canopy transpiration model for greenhouse b. chinensis was established, based on penmam-monteith transpiration model. this established model was validated by the experimental data of independent samples in a single greenhouse. the results showed that in lower reaches of ...200920030149
differential expression of salt tolerance related genes in brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee.we examined salt tolerance responsive genes in pak-choi under salt stress and analyze their potential function. the mrna differential display was used to screen the transcript derived fragments (tdfs) related to salinity tolerance in tolerant and moderately tolerant pak-choi germplasm. seventy-eight primer combinations generated 101 differential cdna fragments, which were divided into 10 expression types. seven cdna sequences (genbank accession nos. dq006915~dq006921) obtained and sequenced were ...200919882760
the construction of a genetic linkage map of non-heading chinese cabbage (brassica campestris ssp. chinensis makino).non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is one of the most important vegetables in eastern china. a genetic linkage map was constructed using 127 doubled haploid (dh) lines, and the dh population was derived from a commercial hybrid "hanxiao" (lines sw-13 x l-118). out of the 614 polymorphic markers, 43.49% were not assigned to any of the linkage groups(lgs). chi-square tests showed that 42.67% markers were distorted from expected mendelian segregation ratios, and ...200919683673
silicon-enhanced resistance to cadmium toxicity in brassica chinensis l. is attributed to si-suppressed cadmium uptake and transport and si-enhanced antioxidant defense capacity.a series of hydroponics experiments were performed to investigate roles of silicon (si) in enhancing cadmium (cd) tolerance in two pakchoi (brassica chinensis l.) cultivars: i.e. cv. shanghaiqing, a cd-sensitive cultivar, and cv. hangyoudong, a cd-tolerant cultivar. plants were grown under 0.5 and 5 mg cd l(-1) cd stress without or with 1.5 mm si. plant growth of the cd-tolerant cultivar was stimulated at the lower cd level, but was decreased at the higher cd level when plants were treated with ...200919616891
cadmium accumulation in pak choi (brassica chinensis l.) and estimated dietary intake in the suburb of hangzhou city, china.the total content of cadmium and its distribution between leaves and roots were investigated for pak choi (brassica chinensis l.), which was grown in a contaminated area of hangzhou city, china. results showed that cadmium concentrations in 90% of samples exceeded the chinese tolerance limit for food. although roots of pak choi had a higher concentration of cadmium than leaves, leaf tissue (the edible part) accumulated over 80% (on average) of the whole plant cadmium burden due to the greater bi ...200924784970
glutamine nitrogen and ammonium nitrogen supplied as a nitrogen source is not converted into nitrate nitrogen of plant tissues of hydroponically grown pak-choi (brassica chinensis l.).many vegetables, especially leafy vegetables, accumulate no(-) (3)-n in their edible portions. high nitrate levels in vegetables constitute a health hazard, such as cancers and blue baby syndrome. the aim of this study was to determine if (1) ammonium nitrogen (nh(+) (4)-n) and glutamine-nitrogen (gln-n) absorbed by plant roots is converted into nitrate-nitrogen of pak-choi (brassica chinensis l.) tissues, and (2) if nitrate-nitrogen (no(-) (3)-n) accumulation and concentration of pak-choi tissu ...200919323774
growth and elemental accumulation of plants grown in acidic soil amended with coal fly ash-sewage sludge co-compost.a greenhouse experiment was conducted to evaluate the growth and heavy-metal accumulation of brassica chinensis and agropyron elongatum in 10 and 25% ash-sludge co-compost (asc)--amended loamy acidic soil (ph 4.51) at two different application rates: 20% and 40% (v/v). soil ph increased, whereas electrical conductivity decreased with the amendment of asc to soil. bioavailable cu, zn, and mn contents of asc-amended soil decreased, whereas ni, pb, and b contents increased. concentrations of bioava ...200919294455
growth inhibition in chinese cabbage (brassica rapa var. chinensis) growth exposed to di-n-butyl phthalate.the toxicity and effects of di-n-butyl phthalate (dbp), an endocrine disruptor, on the growth of chinese cabbage (brassica rapa var. chinensis) were studied. etiolation occurred on leaves of chinese cabbage plant treated with 50mg/l of dbp for 42 d. dbp even below 1mg/l had a significant effect on the concentration of chlorophyll in chinese cabbage and the biomass showed a severe decrease under treatment with more than 30 mg/l of dbp. at a concentration below 1mg/l of dbp, no significant differe ...200918678443
phytotoxicity and speciation of copper, zinc and lead during the aerobic composting of sewage sludge.the content and speciation of heavy metals in composted sewage sludge is the main cause of negative impacts on environment and health of animal and human. an aerobic composting procedure was conducted to investigate the influences of some key parameters on phytotoxicity and speciation of cu, zn and pb during sewage sludge composting. the ph value reached the optimal range for development of microorganisms, and content of organic matter (om) and dissolved organic carbon (doc) decreased with the c ...200918703282
effect of composting process on phytotoxicity and speciation of copper, zinc and lead in sewage sludge and swine manure.the concentration and bioavailability of heavy metals in composted organic wastes have negative environmental impacts following land application. aerobic composting procedures were conducted to investigate the influences of selected parameters on heavy metal speciation and phytotoxicity. results showed that both of sewage sludge (ssc) and swine manure (smc) composting systems decreased the ph, the content of organic matter (om) and dissolved organic carbon (doc), and total amounts of cu, zn and ...200918778928
the lcb2 subunit of the sphingolip biosynthesis enzyme serine palmitoyltransferase can function as an attenuator of the hypersensitive response and bax-induced cell death.previous results showed that expression of the gene encoding the long-chain base2 (lcb(2)) subunit of serine palmitoyltransferase (spt), designated bclcb(2), from nonheading chinese cabbage (brassica campestris ssp. chinensis) was up-regulated during hypersensitive cell death (hcd) induced by the phytophthora boehmeriae elicitor pb90. overexpression of bclcb(2) in nicotiana tabacum leaves suppressed the hcd normally initiated by elicitors and pb90-triggered h(2)o(2) accumulation. bclcb(2) also f ...200919076721
bcmf9, a novel polygalacturonase gene, is required for both brassica campestris intine and exine formation.the polygalacturonase (pg) gene family has been found to be enriched in pollen of several species; however, little is currently known about the function of the pg gene in pollen development. to investigate the exact role that the pg gene has played in pollen development and about this family in general, one putative pg gene, brassica campestris male fertility 9 (bcmf9), was isolated from chinese cabbage (brassica campestris ssp. chinensis, syn. b. rapa ssp. chinensis) and characterized.200919815569
occurrence of clubroot on pak-choi caused by plasmodiophora brassicae.clubroot symptoms occurred severely on roots of pak-choi (brassica campestris ssp. chinensis) grown in greenhouses in gwangju city, gyeonggi province, korea in september, 2008. the incidence of the disease symptoms reached as high as 90% in three greenhouses investigated. the root galls collected from the greenhouses were sectioned using a scalpel and observed by light microscope. many resting spores were found in the cells of the root gall tissues. suspension of resting spores was prepared from ...200923983511
conversion of squid pen by pseudomonas aeruginosa k187 fermentation for the production of n-acetyl chitooligosaccharides and biofertilizers.pseudomonas aeruginosa k187, a protease- and chitinase-producing bacterium, exhibited protease and chitinase activity after three and five days of incubation, respectively. the protease and chitinase were both produced by using 1% squid pen powder (spp) (w/v) as sole carbon and nitrogen source. after fermentation, the deproteinization rate of the recovered squid pen gradually increased up to 68% on the fourth day. after five days of fermentation, the production of glcnac, (glcnac)(2), (glcnac)(3 ...201020206919
diffusion and accumulation in cultivated vegetable plants of di-(2-ethylhexyl) phthalate (dehp) from a plastic production factory.di-(2-ethylhexyl) phthalate (dehp) concentrations in the atmosphere and in four vegetable crops including brassica chinensis l. (bok choy), brassica campestris l. (field mustard), vigna unguiculata walp. (cowpea), and solanum melongena l. (eggplant) cultivated on land surrounding a plastic production factory were determined. the air dehp concentrations (means) at the sites 0.2, 0.4, 0.8, and 1.6 km away from the plastic production building were about 9.4-12.8, 5.8-9.6, 1.6-5.0, and 0.04-0.27 mic ...201020496185
screening of dried plant seed extracts for adiponectin production activity and tumor necrosis factor-alpha inhibitory activity on 3t3-l1 adipocytes.to search for dried plant seeds with potent anti-diabetes activity, we conducted a large scale screening for inhibitory activity on tumor necrosis factor-alpha and facilitating activity on adiponectin production in vitro. these activities in 3t3-l1 adipocytes were screened from ethanol extracts of 20 kinds of dried plant seed marketed in japan. komatsuna (brassica rapa var. perviridis), common bean (phaseolus vulgaris l.), qing geng cai (brassica rapa var. chinensis), green soybean (glycine max) ...201020717728
characterization of cd- and pb-resistant fungal endophyte mucor sp. cbrf59 isolated from rapes (brassica chinensis) in a metal-contaminated soil.to better understand the characteristics of fungal endophytes in the development of effective phytoremediation of heavy metals, the objectives of this study were to isolate a fungal endophyte tolerant cd and pb from rape roots grown in a heavy metal-contaminated soil, to characterize the metal-resistant fungal endophyte, and to assess its potential applications in removal of cd and pb from contaminated solutions and experimental soil. the isolate cbrf59 was identified as mucor sp. based on morph ...201020956060
transgenic brassica chinensis plants expressing a bacterial coda gene exhibit enhanced tolerance to extreme temperature and high salinity.transgenic brassica compestris l. spp. chinensis plants expressing a choline oxidase (coda) gene from arthrobacter globiformis were obtained through agrobacterium tumefaciens-mediated transformation. in the transgenic plants, coda gene expression and its product transportation to chloroplasts were detected by the enzyme-linked immunosorbent assay (elisa) examination, immunogold localization, and (1)h-nuclear magnetic resonance ((1)h-nmr). stress tolerance was evaluated in the t(3) plants under e ...201021043054
[allelopathy of andrographis paniculata vegetative].andrographis paniculata at vegetative stage were analyzed for the allelopathic effect on cabbage (brassica chinensis), radis (raphanus sativus), and desmodium styracifolium, and provided the theory reference for their application of compounding planting pattern in practice.201021548354
elucidating the process of co-composting of biosolids and spent activated clay.this study elucidates the co-composting of biosolids and spent activated clay (sac) using physio-chemical, bioassay, and spectroscopic methods. a pilot-scale pile of blended limed biosolids, sac, and rice husk was composted for 15weeks. the changes in temperature, ph, fourier-transform infrared (ft-ir) spectra, c/n, and germination index (gi) of chinese cabbage (brassica chinensis) seeds with time support the goal of producing a mature compost with a decline in the sac acidity of associated with ...201020594829
a polygalacturonase inhibitory protein gene (bcmf19) expressed during pollen development in chinese cabbage-pak-choi.the level of polygalacturonase inhibitory protein (pgip) genes involved in pollen development remains unclear. characterization of the different pgip genes that are expressed in pollen is necessary in understanding the similarities and differences of functions between the members of this gene family, as well as the underlying mechanism of pollen development. a gene-encoding putative pgip, bcmf19 was successfully cloned on a cdna-amplified fragment length polymorphism fragment after it was found ...201120373034
[transformation of copper and chromium in co-contaminated soil and its influence on bioavailability for pakchoi (brassica chinensis)].heavy metal fractions in soil play an important role in the bioavailability for plants. a pot experiment was carried out to study the transformation and bioavailability for pakchoi of exogenetic copper and hexavalent chromium in co-contaminated soil. the results showed that one month after tested heavy metals were added into soil, the chromium was present mainly in residual and organic bonded form while copper was mainly bound to hydrated oxides of iron and manganese as well as residual form. lo ...201122279932
[effect and mechanism of immobilization of cadmium and lead compound contaminated soil using new hybrid material].the effect of new hybrid material and its compound treatments with phosphate on immobilization of cadmium and lead in contaminated soil was investigated using a pot-culture experiment, and the immobilization mechanism of hybrid material was clarified through analysis of heavy metal fractions, sorption equilibration experiment and x-ray photoelectron spectroscopy (xps). the single treatments of hybrid material could not significantly promote growth of brassica chinensis, while the compound treatm ...201121528587
phytotoxicity of pfos and pfoa to brassica chinensis in different chinese soils.pfos and pfoa are potential persistent organic pollutants that have raised many concerns in recent years. research focusing on phytotoxicity of pfos and pfoa to higher plants is necessary for their risk assessments. however, few toxicity data exist for pfos or pfoa and higher plants. here we investigated phytotoxicity of pfos and pfoa to brassica chinensis root growth in six different chinese soils varying widely in soil properties using a standardized root length assay. the effective concentrat ...201121440938
growth promotion of chinese cabbage and arabidopsis by piriformospora indica is not stimulated by mycelium-synthesized auxin.piriformospora indica, an endophytic fungus of the order sebacinales, interacts with the roots of a large variety of plant species. we compared the interaction of this fungus with chinese cabbage (brassica campestris subsp. chinensis) and arabidopsis seedlings. the development of shoots and roots of chinese cabbage seedlings was strongly promoted by p. indica and the fresh weight of the seedlings increased approximately twofold. the strong stimulation of root hair development resulted in a bushy ...201121375386
fate and distribution of nitrogen in soil and plants irrigated with landfill leachate.landfill leachate contains a high concentration of ammoniacal substances which can be a potential supply of n for plants. a bioassay was conducted using seeds of brassica chinensis and lolium perenne to evaluate the phytotoxicity of the leachate sample. a soil column experiment was then carried out in a greenhouse to study the effect of leachate on plant growth. two grasses (paspalum notatum and vetiver zizanioides) and two trees (hibiscus tiliaceus and litsea glutinosa) were irrigated with leac ...201121349695
bcmf20, a putative pollen-specific transcription factor from brassica campestris ssp. chinensis.the arabidopsis transcription factor (at1g26610) was shown to be down-regulated in male sterile lines compared to their maintainer line in chinese cabbage (brassica campestris ssp. chinensis) in our previous study. the bcmf20 gene, homologue of the at1g26610 gene was isolated from chinese cabbage. it encodes a putative c2h2 zinc finger protein. the spatial and temporal expression patterns examined by qrt-pcr and in situ hybridization, indicated bcmf20 is specifically expressed in the developing ...201121327825
isolation and characterization of a novel bcmf14 gene from brassica campestris ssp. chinensis.a putative ralf (rapid alkalinization factor)-like gene (genbank accession number ef523517), named bcmf14, was isolated from chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) by rapid amplification of cdna ends (race) based on a cdna-aflp differential fragment exclusively expressed in fertile line. semi-quantitative reverse transcription-polymerase chain reaction (rt-pcr) discovered that bcmf14 was prominently expressed in stage four and five flower buds ...201120857218
A novel cold-inducible gene from Pak-choi (Brassica campestris ssp. chinensis), BcWRKY46, enhances the cold, salt and dehydration stress tolerance in transgenic tobacco.WRKY TFs belong to one of the largest families of transcriptional regulators in plants and form integral parts of signaling webs that modulate many plant processes. BcWRKY46, a cDNA clone encoding a polypeptide of 284 amino acids and exhibited the structural features of group III of WRKY protein family, was isolated from the cold-treated leaves of Pak-choi (Brassica campestris ssp. chinensis Makino, syn. B. rapa ssp. chinensis) using the cDNA-AFLP technique. Expression of this gene was induced q ...201121938429
Effect of temperature on cortical infection by Plasmodiophora brassicae and clubroot severity.A study was conducted to assess the effect of temperature on infection and development of Plasmodiophora brassicae in the root cortex of Shanghai pak choy (Brassica rapa subsp. chinensis) and on subsequent clubroot severity. Ten-day-old seedlings were grown individually, inoculated with resting spores, and maintained in growth cabinets at 10, 15, 20, 25, and 30?C. Seedlings were harvested at 2-day intervals, starting 8 days after inoculation (DAI) and continuing until 42 DAI. Roots were assessed ...201121864086
[effects of organic fertilization on arsenic absorption of pakchoi (brassica chinensis) on arsenic-contaminated red soil].a pot experiment with arsenic-contaminated red soil was conducted to study the effects of applying pig dung and chicken manure on the growth and arsenic absorption of pakchoi (brassica chinensis), and on soil available arsenic. applying pig dung and chicken manure to the arsenic-contaminated red soil increased the biomass of pakchoi to some extent. comparing with the control, applying pig dung increased the pakchoi biomass significantly (p < 0.05). the soil available arsenic content after applyi ...201121548308
Altered transfer of heavy metals from soil to Chinese cabbage with film mulching.The influence of film mulching on the migration of metals from soil to cabbage was investigated. Following a 50-day growth in field plots mulched or unmulched, root-zone soils and Chinese cabbage (Brassica chinensis L.) were sampled for metal analysis. Mulching slightly decreased the soil mobile (acid-extractable) Cd, but increased its transfer from root to the cabbage parts. As an essential element, Cu was readily transferred to the cabbage parts. While mulching decreased the soil mobile Zn, re ...201222036267
[effects of nitrogen regulators on fertilizer nitrogen transformation in meadow cinnamon soil and on pakchoi growth].soil incubation test and pot experiment were conducted to investigate the effects of dicyandiamide (dcd) and its combination with nano-carbon on the transformation of fertilizers (urea and ammonium bicarbonate) nitrogen (n) in meadow cinnamon soil, a typical soil type in north china plain, and on the growth of pakchoi (brassica chinensis). in the first two weeks after applying urea and ammonium bicarbonate, the soil nh4+-n and no3(-)-n contents varied greatly, but little variation was observed s ...201223286007
food safety assessment of planting patterns of four vegetable-type crops grown in soil contaminated by electronic waste activities.a field experiment was conducted to assess the effect of crop and planting pattern on levels of cadmium (cd), lead (pb), and copper (cu) in crops grown in soil contaminated by electronic waste. the crops were maize (zea mays l. var. shentian-1), tomato (solanum lycopersicum l. var. zhongshu-4), cabbage (brassica oleracea l. var. jingfeng-1), and pakchoi (brassica chinensis (l.) makino. var. youdonger-hangzhou). the planting patterns were crop monoculture, crop co-planted with a legume, and crop ...201222054567
[effects of different applied nitrogen forms on pakchoi (brassica chinensis) growth and its carbon and nitrogen accumulation].a hydroponic experiment was conducted to study the effects of different applied nitrogen forms, i.e., ammonium, nitrate, glycine, glutamine, alanine, bovine serum albumin (bsa), mixture of glycine and nitrate, and mixture of bsa and nitrate, on the growth and carbon and nitrogen accumulation of pakchoi (brassica chinensis). the significant differences were observed in the b. chinensis dry mass, fresh mass, carbon and nitrogen accumulation, and soluble protein, soluble sugar, and free amino acid ...201222803472
effects of phosphorus amendments and plant growth on the mobility of pb, cu, and zn in a multi-metal-contaminated soil.phosphorus amendments have been widely and successfully used in immobilization of one single metal (e.g., pb) in contaminated soils. however, application of p amendments in the immobilization of multiple metals and particularly investigations about the effects of planting on the stability of the initially p-induced immobilized metals in the contaminated soils are far limited.201222161145
bcnrt1, a plasma membrane-localized nitrate transporter from non-heading chinese cabbage.a nitrate transporter, bcnrt1, was isolated from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) cultivar 'suzhouqing'. the full-length cdna was obtained using the rapid amplification of cdna ends technique and contains an open reading frame of 1,770 bp that predicts a protein of 589 acid residues that possesses 12 putative transmembrane domains. using the gus marker gene driven by the bcnrt1 promoter, we found bcnrt1 expression to be concentrated in primary and lateral r ...201222539185
evaluation of iodide and iodate for adsorption-desorption characteristics and bioavailability in three types of soil.adsorption-desorption of iodine in two forms, viz., iodide (i(-)) and iodate (io (3) (-) ), in three types of soil were investigated. the soils were: red soil developed on quaternary red earths (req)- clayey, kaolintic thermic plinthite aquult, inceptisol soil (is) and alluvial soil (as)-fluvio-marine yellow loamy soil. the isothermal curves of iodine adsorption on soils were described by langmuir and freundlich equation, and the maximum adsorption values (y (m)) were obtained from the simple la ...201222038267
permissible value for vanadium in allitic udic ferrisols based on physiological responses of green chinese cabbage and soil microbes.greenhouse experiments were conducted to study the permissible value of vanadium (v) based on the growth and physiological responses of green chinese cabbage (brassica chinensis l.), and effects of v on microbial biomass carbon (mbc) and enzyme activities in allitic udic ferrisols were also studied. the results showed that biomass of cabbage grown on soil treated with 133 mg v kg(-1) significantly decreased by 25.1% compared with the control (p < 0.05). vanadium concentrations in leaves and root ...201221898106
microtubule and male sterility in a gene-cytoplasmic male sterile line of non-heading chinese cabbage.microtubules are the basic components of the cytoskeleton in eukaryotic cells and are made up of 13 parallel protofilaments, each composed of α- and β-tubulin unit molecules aligned along the longitudinal axis of the microtubule.201222581783
sequence characterization and expression pattern of bcmf21, a novel gene related to pollen development in brassica campestris ssp. chinensis.in this report a full length cdna, brassica campestris male fertile 21 (bcmf21) was successfully isolated from one of the cdna-amplified fragment length polymorphism (cdna-aflp) transcript-derived fragments (tdfs), bbp10, that was found down-regulated in the flower buds of sterile plants in brassica campestris l. ssp. chinensis makino genic male sterile (gms) ab line system (bcajh 97-01a/b). bcmf21 protein structure analysis showed a signal peptide at the n-terminus; two protein kinase c phospho ...201222311045
effect of some plant growth regulators on lindane and alpha-endosulfan toxicity to brassica chinensis.the effect of indolebutyric acid (iba) and gibberellic acid (ga3), to alleviate the organochlorine phytotoxicity were studied in brassica chinensis. presence of organochlorine decreased brassica chinensis seedlings growth in contaminated alkaline soil. one mg l(-1) iba could enhance 14 and 26% shoot and root length of b. chinensis seedlings grown at 40 mg kg(-1) lindane contaminated soil, respectively. ten mg l(-1) iba also increased 80 and 40% root fresh weight of seedling grown in 40 mg kg(-1) ...201223360012
[transformation and influences of copper and selenium fractions on heavy metals bioavailability in co-contaminated soil].pot experiments and laboratory analysis methods were used to investigate the form transformation of additional copper and selenium and their bioavailability for pakchoi (brassica chinensis) in co-contaminated soil. the results showed that cu mainly existed in residual bound form, while selenium was present mainly in organic bound and residual form in the uncontaminated soil. in the contaminated soil, copper was mainly bounded to hydrated oxides of iron and manganese, while se was in exchangeable ...201223213910
reducing the bioavailability of pcbs in soil to plant by biochars assessed with triolein-embedded cellulose acetate membrane technique.coupling with triolein-embedded cellulose acetate membrane (tecam) technique, hydroxypropyl β-cyclodextrins (hpcd) extraction method, and the greenhouse pot experiments, the influences of biochars on polychlorinated biphenyls (pcbs) bioavailability in soil to plant (brassica chinensis l. and daucus carota) were investigated. addition of 2% biochars to soils significantly reduced the uptake of pcbs in plant, especially for di-, tri- and tetra-chlorobiphenyls. pcbs concentrations in the roots of b ...201323279904
bcmf11, a novel non-coding rna gene from brassica campestris, is required for pollen development and male fertility.key message : bcmf11 as a non-coding rna gene has an essential role in pollen development, and might be useful for regulating the pollen fertility of crops by antisense rna technology. we previously identified a 828-bp full-length cdna of bcmf11, a novel pollen-specific non-coding mrna-like gene from chinese cabbage (brassica campestris l. ssp. chinensis makino). however, little information is known about the function of bcmf11 in pollen development. to investigate its exact biological roles in ...201323064614
effect of modified atmosphere packaging on the quality and bioactive compounds of chinese cabbage (brasicca rapa l. ssp. chinensis).the perishability of brassica chinensis poses a major challenge to distribution and marketing. the aim of this work was to select a suitable modified atmosphere packaging to retain the overall quality and bioactive compounds during storage.201323254451
rape (brassica chinensis l.) seed germination, seedling growth, and physiology in soil polluted with di-n-butyl phthalate and bis(2-ethylhexyl) phthalate.phthalic acid esters (paes) pollution in agricultural soils caused by widely employed plastic products is becoming more and more widespread in china. paes polluted soil can lead to phytotoxicity in higher plants and potential health risks to human being. we evaluated the individual toxicity of di-n-butyl phthalate (dnbp) and bis(2-ethylhexyl) phthalate (dehp), two representative paes, to sown rape (brassica chinensis l.) seeds within 72 h (as germination stage) and seedlings after germination fo ...201323389857
identification of gene expression profile during fertilization in brassica campestris subsp. chinensis.fertilization is controlled by a complex gene regulatory network. to study the fertilization mechanism, we determined time courses of the four developmental stages of fertilization in chinese cabbage pak-choi (brassica campestris subsp. chinensis) by cytological observation. we then used the arabidopsis ath1 microarray to characterize the gene expression profiles of pollinated and unpollinated pistils in b. campestris subsp. chinensis. the result showed 44 up-regulated genes and 33 down-regulate ...201323379337
the dynamic growth exhibition and accumulation of cadmium of pak choi (brassica campestris l. ssp. chinensis) grown in contaminated soils.the accumulation of heavy metals, especially cadmium (cd), in leafy vegetables was compared with other vegetables. pak choi (brassica campestris l. ssp. chinensis) is a leafy vegetable consumed in taiwan and its safety for consumption after growing in contaminated soils is a public concern. a pot experiment (50 days) was conducted to understand the dynamic accumulation of cd by pak choi grown in artificially contaminated soils. the edible parts of pak choi were sampled and analyzed every 2-3 day ...201324284350
characterization and co-expression analysis of wrky orthologs involved in responses to multiple abiotic stresses in pak-choi (brassica campestris ssp. chinensis).the wrky transcription factor is an important member of the stress-related transcription factors, which mediate diverse abiotic stresses in many plants. however, up until now, the number of wrky members, and the regulatory mechanisms involved in abiotic stress responses in pak-choi (brassica campestris ssp. chinensis), remained unknown.201324267479
[differences of selenium uptake pattern of pakchoi and the possible mechanism when amended with selenate and selenite].in order to produce selenium-enriched crops and provide a theoretical basis of phytoremediation to selenium contaminated soil, pot experiment and lab analysis were carried out to examine the differences of selenium uptake pattern and bioavailability to pakchoi (brassica chinensis l.) when amended with selenate and selenite. meanwhile, dynamic changes of soil available se was analyzed to explore the reason. the results showed that no significant differences in pakchoi shoot biomass between selena ...201324191579
comparison of ascorbic acid biosynthesis in different tissues of three non-heading chinese cabbage cultivars.ascorbic acid (l-asa) is an important antioxidant in plants and humans. vegetables are one of the main sources of ascorbic acid for humans. for instance, non-heading chinese cabbage (brassica campestris ssp. chinensis makino) is considered as one of the most important vegetables in south china. to elucidate the mechanism by which asa accumulates, we systematically investigated the expression profiles of d-mannose/l-galactose pathway-related genes. we also investigated the recycling-related genes ...201324157701
chemical stabilization of cadmium in acidic soil using alkaline agronomic and industrial by-products.in situ immobilization of heavy metals using reactive or stabilizing materials is a promising solution for soil remediation. therefore, four agronomic and industrial by-products [wood biochar (wb), crushed oyster shell (os), blast furnace slag (bfs), and fluidized-bed crystallized calcium (fbcc)] and caco3 were added to acidic soil (cd = 8.71 mg kg(-1)) at the rates of 1%, 2%, and 4% and incubated for 90 d. chinese cabbage (brassica chinensis l.) was then planted in the soil to test the cd uptak ...201323947715
identification of micrornas potentially involved in male sterility of brassica campestris ssp. chinensis using microrna array and quantitative rt-pcr assays.micrornas (mirnas) are a class of newly identified, noncoding, small rna molecules that negatively regulate gene expression. many mirnas are reportedly involved in plant growth, development and stress response processes. however, their roles in the sexual reproduction mechanisms in flowering plants remain unknown. pollen development is an important process in the life cycle of a flowering plant, and it is closely related to the yield and quality of crop seeds. this study aimed to identify mirnas ...201323864334
subcellular distribution of metals within brassica chinensis l. in response to elevated lead and chromium stress.differential centrifugation and synchrotron radiation x-ray fluorescence spectroscopy (srxrf) microprobe were used to study the distribution of the elements in tissue cross sections of pakchoi ( brassica chinensis l.) under stress of elevated pb and cr. subcellular fractionation of the different tissues grown in a nutrient solution containing 200 mg l(-1) pb or 5 mg l(-1) cr showed that 86.7 and 76.3% of the pb that accumulated in the roots and shoots, respectively, was contained in the cell wal ...201323621278
organic nitrogen components in soils from southeast china.to investigate the amounts of extractable organic nitrogen (eon), and the relationships between eon and total extractable nitrogen (ten), especially the amino acids (aas) adsorbed by soils, and a series of other hydrolyzed soil nitrogen indices in typical land use soil types from southeast china. under traditional agricultural planting conditions, the functions of eon, especially aas in the rhizosphere and in bulk soil zones were also investigated.201323549843
changes in the relative abundance and movement of insect pollinators during the flowering cycle of brassica rapa crops: implications for gene flow.the potential movement of transgenes from genetically modified crops to non-genetically modified crops via insect-mediated pollen dispersal has been highlighted as one of the areas of greatest concern in regards to genetically modified crops. pollen movement depends sensitively on spatial and temporal variation in the movement of insect pollinators between crop fields. this study tested the degree of variation in the diversity and relative abundance of flower-visiting insects entering versus lea ...201323937538
uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for lowering dehp content of intercropped vegetables.uptake of di(2-ethylhexyl) phthalate (dehp) by the plant benincasa hispida and its use for topical phytoremediation were investigated by cultivation of plants in dehp-contaminated environments. the results showed that major plant organs of b. hispida , including leaves, stems, and fruits, readily absorbed dehp from the air. the amount of dehp that accumulated in leaves, stems, and fruits was mainly dependent upon exposure time, and most dehp accumulated in their inner tissues. a single plant of ...201323675826
bcpmi2, isolated from non-heading chinese cabbage encoding phosphomannose isomerase, improves stress tolerance in transgenic tobacco.phosphomannose isomerase (pmi) is an enzyme that catalyses the first step of the l-galactose pathway for ascorbic acid (asa) biosynthesis in plants. to clarify the physiological roles of pmi in asa biosynthesis, the cdna sequence of pmi was cloned from non-heading chinese cabbage (brassica campestris ssp. chinensis makino) and overexpressed in tobacco transformed with agrobacterium tumefaciens. the asa and soluble sugar contents were lower in 35s::bcpmi2 tobacco than in wild-type tobacco. howeve ...201424430300
anthocyanin accumulation and transcriptional regulation of anthocyanin biosynthesis in purple bok choy (brassica rapa var. chinensis).bok choy (brassica rapa var. chinensis) is an important dietary vegetable cultivated and consumed worldwide for its edible leaves. the purple cultivars rich in health-promoting anthocyanins are usually more eye-catching and valuable. fifteen kinds of anthocyanins were separated and identified from a purple bok choy cultivar (zi he) by high-performance liquid chromatography-electrospray ionization tandem mass spectrometry. to investigate the molecular mechanisms underlying anthocyanin accumulatio ...201425419600
pectate lyase-like10 is associated with pollen wall development in brassica campestris.pectate lyase-like10 (pll10) was previously identified as one of the differentially expressed genes both in microspores during the late pollen developmental stages and in pistils during the fertilization process in chinese cabbage (brassica campestris ssp. chinensis). here, antisense-rna was used to study the functions of bcpll10 in chinese cabbage. abnormal pollen was identified in the transgenic lines (bcpll10-4, -5, and -6). in fertilization experiments, fewer seeds were harvested when the an ...201424773757
petroleum contamination of soil and water, and their effects on vegetables by statistically analyzing entire data set.aliphatic hydrocarbons have been used to assess total oil concentrations, petroleum sources, and petroleum degradation. in this study, surface soil, groundwater, surface water, and vegetables were collected from the outskirts of xi'an, the largest city in northwestern china, and the samples were analyzed for aliphatic hydrocarbon contents. the concentrations of n-alkanes were 1.06-4.01 μg/g in the soil. the concentrations and the geochemical characteristics of n-alkanes showed that the low carbo ...201424468500
bioactive metabolites isolated from penicillium sp. yy-20, the endophytic fungus from ginkgo biloba.six known metabolites, adenosine (1), methyl β-d-ribofuranoside (2), adenine (3), 2'-deoxyadenosine (4), 3-methylpiperazine-2,5-dione (5) and 2'-deoxyuridine (6), were isolated from the extracts of the endophytic fungus penicillium sp. yy-20 isolated from the root of ginkgo biloba, and their structures were elucidated by spectroscopic methods. the antioxidant and growth-promoting activities of these compounds were first evaluated. the results indicated that compounds 1, 3 and 4 exhibited potenti ...201424144081
exposure factors for wastewater-irrigated asian vegetables and a probabilistic rotavirus disease burden model for their consumption.many farmers in water-scarce regions of developing countries use wastewater to irrigate vegetables and other agricultural crops, a practice that may expand with climate change. there are a number of health risks associated with wastewater irrigation for human food crops, particularly with surface irrigation techniques common in the developing world. the world health organization (who) recommends using quantitative microbial risk assessment (qmra) to determine if the irrigation scheme meets healt ...201424576153
identification of differentially accumulating pistil proteins associated with self-incompatibility of non-heading chinese cabbage.non-heading chinese cabbage (brassica campestris l. ssp. chinensis makino), an important vegetable crop in china, exhibits a typical sporophytic self-incompatibility (si) system. to better understand the mechanism of si response and identify potential candidate proteins involved in the si system of this vegetable crop, the proteomic approach was taken to identify differential accumulating pistil proteins. pistils were collected at 0 h and 2 h after self-pollination at anthesis in self-incompatib ...201423581423
evaluation of selenium species in selenium-enriched pakchoi (brassica chinensis jusl var parachinensis (bailey) tsen & lee) using mixed ion-pair reversed phase hplc-icp-ms.hplc-icp-ms based on ion-paired reversed phase chromatography for the selenium speciation using the mixture of 1-butanesulfonic acid (ba) and trifluoroacetic acid (tfa) as the mixed ion-pairing reagents was developed and applied to selenium-enriched pakchoi (brassica chinensis jusl var parachinensis (bailey) tsen & lee). several conditions of ion-paired reversed phase hplc-icp-ms, such as ph of the mobile phase, concentration of ion pairing reagents, types and length of analytical column, and fl ...201424128538
directional transfer of a multiple-allele male sterile line in brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee.to produce hybrid seeds of wutacai (brassica campestris l. ssp. chinensis (l.) makino var. rosularis tsen et lee), a "directional transfer program" was designed to breed the multiple-allele male sterile line of wutacai. a multiple-allele male sterile line of naibaicai (brassica campestris l. ssp. chinensis l., s01) was used as the male sterile resource, and an inbred line of wutacai (wt01) was used as the target line. recurrent backcrossing was employed to transfer the male sterility and other b ...201424987301
identification of novel and conserved mirnas involved in pollen development in brassica campestris ssp. chinensis by high-throughput sequencing and degradome analysis.micrornas (mirnas) are endogenous, noncoding, small rnas that have essential regulatory functions in plant growth, development, and stress response processes. however, limited information is available about their functions in sexual reproduction of flowering plants. pollen development is an important process in the life cycle of a flowering plant and is a major factor that affects the yield and quality of crop seeds.201424559317
antioxidant enzymes regulate reactive oxygen species during pod elongation in pisum sativum and brassica chinensis.previous research has focused on the involvement of reactive oxygen species (ros) in cell wall loosening and cell extension in plant vegetative growth, but few studies have investigated ros functions specifically in plant reproductive organs. in this study, ros levels and antioxidant enzyme activities were assessed in pisum sativum and brassica chinensis pods at five developmental stages. in juvenile pods, the high levels of o2.- and .oh indicates that they had functions in cell wall loosening a ...201424503564
effects of zno nanoparticles and microwave heating on the sterilization and product quality of vacuum-packaged caixin.the sterilization of vacuum-packaged caixin (brassica chinensis l.), which is a green-leafy vegetable and also a low-acid food, remains a difficult problem. in this study, effects of zno nanoparticles and microwave heating on the sterilization and product quality of vacuum-packaged caixin were investigated.201424464779
proteomic analysis of pakchoi leaves and roots under glycine-nitrogen conditions.the physiological and differential proteomic responses of pakchoi leaves and roots to glycine-nitrogen (gly-n) treatments were determined. two pakchoi (brassica campestris ssp. chinensis l. makino. var. communis tsen et lee) cultivars, 'huawang' and 'wuyueman', were grown under sterile hydroponic conditions with different n forms (gly-n and nitrate-n). gly-n-treated pakchoi exhibited decreased fresh weights, total n uptake, leaf areas, and net photosynthetic rates than those treated with nitrate ...201424429133
bcmf21 is important for pollen development and germination in brassica campestris ssp. chinensis.brassica campestris male fertility 21 (bcmf21) was previously isolated from the flower buds of chinese cabbage (brassica campestris l. ssp. chinensis makino, syn. b. rapa ssp. chinensis) and expressed specifically in tapetum and microspores during the meiosis stage and the uninucleate stage of microspore development. here, we used antisense rna technology to knock down the expression level of bcmf21 in b. campestris and analyzed the phenotype of the transgenic plants. alexander staining and scan ...201424323195
phytoavailability of cadmium (cd) to pak choi (brassica chinensis l.) grown in chinese soils: a model to evaluate the impact of soil cd pollution on potential dietary toxicity.food chain contamination by soil cadmium (cd) through vegetable consumption poses a threat to human health. therefore, an understanding is needed on the relationship between the phytoavailability of cd in soils and its uptake in edible tissues of vegetables. the purpose of this study was to establish soil cd thresholds of representative chinese soils based on dietary toxicity to humans and develop a model to evaluate the phytoavailability of cd to pak choi (brassica chinensis l.) based on soil p ...201425386790
evaluation of phytoavailability of heavy metals to chinese cabbage (brassica chinensis l.) in rural soils.this study compared the extractability of cd, cu, ni, pb, and zn by 8 extraction protocols for 22 representative rural soils in taiwan and correlated the extractable amounts of the metals with their uptake by chinese cabbage for developing an empirical model to predict metal phytoavailability based on soil properties. chemical agents in these protocols included dilute acids, neutral salts, and chelating agents, in addition to water and the rhizon soil solution sampler. the highest concentrations ...201425295297
the effectiveness of spent coffee grounds and its biochar on the amelioration of heavy metals-contaminated water and soil using chemical and biological assessments.spent coffee grounds (scg) and charred spent coffee grounds (scg-char) have been widely used to adsorb or to amend heavy metals that contaminate water or soil and their success is usually assessed by chemical analysis. in this work, the effects of scg and scg-char on metal-contaminated water and soil were evaluated using chemical and biological assessments; a phytotoxicity test using bok choy (brassica campestris l. ssp. chinensis jusl.) was conducted for the biological assessment. when scg and ...201425242543
transformation of heavy metal fractions on soil urease and nitrate reductase activities in copper and selenium co-contaminated soil.this study aims to explore the effects of the distribution, transformation and bioavailability of different fractions of copper (cu) and selenium (se) in co-contaminated soils on soil enzymes, providing references for the phytoremediation of contaminated areas and agriculture environmental protection. pot experiments and laboratory analysis were used to investigate the transformation and bioavailability of additional cu and se for pakchoi (brassica chinensis) in co-contaminated soil. in the unco ...201425193883
expression analysis of self-incompatibility-associated genes in non-heading chinese cabbage.in brassicaceae, a self-incompatibility (si) system mediates pollen-pistil interactions. self-pollen could be recognized and rejected by incompatible pistils. several components involved in the si response have been determined, including s-locus receptor kinase (srk), s-locus cysteine-rich protein/s-locus protein 11, and arm repeat-containing protein 1 (arc1). however, the components involved in the si system of brassicaceae are not fully understood. here, we detected expression patterns of 24 s ...201425062491
molecular cloning and expression of the male sterility-related ctyabby1 gene in flowering chinese cabbage (brassica campestris l. ssp chinensis var. parachinensis).expression of the yabby gene family in the abaxial surface of lateral plant organs determines abaxial destiny of cells, enhances growth and expansion of lateral organs, and plays an important role in polar establishment of lateral organs. however, the yabby gene has not been studied in male sterility and fertility restoration. we homologously cloned the ctyabby1 gene of male-sterile tc1 in brassica campestris l. ssp chinensis var. parachinensis; its expression was analyzed by real-time pcr. a 93 ...201425036178
effects of spray application of lanthanum and cerium on yield and quality of chinese cabbage (brassica chinensis l) based on different seasons.the use of single light rare earth elements in agriculture was studied using the application of single rare earth elements (la and ce) to chinese cabbage (brassica chinensis l.) in field plot experiments where the soil conditions remained the same but the season (spring or autumn) in which application occurred was altered. the results showed that the chinese cabbage's nutrition quality between the two seasons had obvious differences. when planted in the autumn, the soluble sugar and vitamin c co ...201425022243
proteomic analysis of non-heading chinese cabbage infected with hyaloperonospora parasitica.downy mildew is a serious fungal disease in non-heading chinese cabbage (brassica campestris ssp. chinensis makino) that is caused by hyaloperonospora parasitica, which infects members of the brassicaceae family. for breeding improvement, researchers must understand the defence mechanisms employed by non-heading chinese cabbage to combat h. parasitica infection. using 2-de protein analysis, we compared the proteomes from leaves of non-heading chinese cabbage seedlings that were infected with h. ...201424334100
persistence and dissipation of chlorpyrifos in brassica chinensis, lettuce, celery, asparagus lettuce, eggplant, and pepper in a greenhouse.the residue behavior of chlorpyrifos, which is one of the extensively used insecticides all around the world, in six vegetable crops was assessed under greenhouse conditions. each of the vegetables was subjected to a foliar treatment with chlorpyrifos. two analytical methods were developed using gas chromatography equipped with a micro-ecd detector (loq = 0.05 mg kg(-1)) and liquid chromatography with a tandem mass spectrometry (loq = 0.01 mg kg(-1)). the initial foliar deposited concentration o ...201424967589
variations in the natural ¹⁵n abundance of brassica chinensis grown in uncultivated soil affected by different nitrogen fertilizers.to further investigate the method of using δ(15)n as a marker for organic vegetable discrimination, the effects of different fertilizers on the δ(15)n in different growing stages of brassica chinensis (b. chinensis) grown in uncultivated soil were investigated with a pot experiment. b. chinensis was planted with uncultivated soil and different fertilizer treatments and then harvested three times in three seasons consecutively. for the spring experiments in the years of 2011 and 2012, the δ(15)n ...201425369912
interplay between orfamides, sessilins and phenazines in the control of rhizoctonia diseases by pseudomonas sp. cmr12a.we investigated the role of phenazines and cyclic lipopeptides (clps) (orfamides and sessilins), antagonistic metabolites produced by pseudomonas sp. cmr12a, in the biological control of damping-off disease on chinese cabbage (brassica chinensis) caused by rhizoctonia solani ag 2-1 and root rot disease on bean (phaseolus vulgaris l.) caused by r. solani ag 4-hgi. a pseudomonas mutant that only produced phenazines suppressed damping-off disease on chinese cabbage to the same extent as cmr12a, whi ...201526085277
effects of inorganic and organic amendments on the uptake of lead and trace elements by brassica chinensis grown in an acidic red soil.a greenhouse study was conducted to investigate the effects of inorganic (phosphate rock, single superphosphate and calcium magnesium phosphate) and organic amendments (peat, straw manure and pig manure) on the uptake of lead (pb) and trace elements by chinese cabbage (brassica chinensis) grown in an acidic red soil. the application of all organic amendments increased the soil ph while inorganic amendments such as single superphosphate did not. both inorganic and organic amendments decreased the ...201524992219
photocatalytic degradation of acephate in pak choi, brassica chinensis, with ce-doped tio2.the photocatalytic degradation of acephate was investigated using ce-doped tio2 (tio2/ce) hydrosol. in contrast to previous research conducted under artificial light in the laboratory, this study investigated the decomposition of acephate in a field trial. the results show that acephate can be efficiently degraded by the tio2/ce system under natural field conditions; the degradation efficiency was affected by the dosage of the photocatalyst and acephate. the optimum dosage of tio2/ce was 2400 g ...201525826101
effects of selenite and selenate application on growth and shoot selenium accumulation of pak choi (brassica chinensis l.) during successive planting conditions.selenate and selenite are two main kinds of inorganic selenium (se) sources in soil, but these substances can pose threats to the environment. phytoextraction is an emerging technology to remove se from polluted soils by using a hyper-accumulator. in this study, a pot experiment was conducted to investigate se phytoextraction potential of pak choi (brassica chinensis l.) and to determine the effects of se on growth and se accumulation of pak choi under successive planting conditions (four crops) ...201525794583
accumulation, allocation, and risk assessment of polycyclic aromatic hydrocarbons (pahs) in soil-brassica chinensis system.farmland soil and leafy vegetables accumulate more polycyclic aromatic hydrocarbons (pahs) in suburban sites. in this study, 13 sampling areas were selected from vegetable fields in the outskirts of xi'an, the largest city in northwestern china. the similarity of pah composition in soil and vegetation was investigated through principal components analysis and redundancy analysis (rda), rather than discrimination of pah congeners from various sources. the toxic equivalent quantity of pahs in soil ...201525679782
molecular cloning and expression analysis of a cu/zn sod gene (bccsd1) from brassica campestris ssp. chinensis.superoxide dismutases (sods) are a family of metalloproteins extensively exists in eukaryote, which plays an essential role in stress-tolerance of higher plants. a full-length cdna encoding cu/zn sod (bccsd1) was isolated from young seedlings of non-heading chinese cabbage (brassica campestris ssp. chinensis) by rapid amplification of cdna ends (race). bioinformatics analysis revealed that bccsd1 belonged to the plant sod super family and had the closest relationship with sod from brassica napus ...201525976826
use of monoclonal antibodies in an elisa for detecting an invasive pest insect, liriomyza trifolii (diptera: agromyzidae).a monoclonal antibody was prepared by the hybridoma technology. it reacted only with the protein of liriomyza trifolii (burgess) and not with that of chromatomyia horticola goureau or liriomyza sativae blanchard in indirect enzyme-linked immunosorbent assay. it was effective even after being diluted more than 8.192×10(6)-fold. the detection sensitivity of the antibody was 31.3 µg/ml under controlled conditions. positive reaction was achieved with all laboratory-reared l. trifolii samples, includ ...201526470159
[identification of soft rot pathogens on chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing].this study aimed to identify soft rot pathogens of chinese cabbage [brassica campestris l. ssp. chinensis (l.) makino var. communis tsen et lee] in beijing.201526939453
genome-wide identification of turnip mosaic virus-responsive micrornas in non-heading chinese cabbage by high-throughput sequencing.turnip mosaic virus (tumv) is the most prevalent viral pathogen infecting most cruciferous plants. micrornas (mirnas) are around 22 nucleotides long non-protein-coding rnas that play key regulatory roles in plants. recent research findings show that mirnas are involved in plant-virus interaction. however we know little about plant defense and viral offense system networks throughout microrna regulation pathway. in this study, two small rna libraries were constructed based on non-heading chinese ...201526115771
assessment of heavy metal pollution in vegetables and relationships with soil heavy metal distribution in zhejiang province, china.there are increasing concerns on heavy metal contaminant in soils and vegetables. in this study, we investigated heavy metal pollution in vegetables and the corresponding soils in the main vegetable production regions of zhejiang province, china. a total of 97 vegetable samples and 202 agricultural soil samples were analyzed for the concentrations of cd, pb, as, hg, and cr. the average levels of cd, pb, and cr in vegetable samples [chinese cabbage (brassica campestris spp. pekinensis), pakchoi ( ...201526013654
effects of dissolved organic matter on uptake and translocation of lead in brassica chinensis and potential health risk of pb.dissolved organic matter (dom) can affect the bioavailability of heavy metals in soil, especially in soils used for vegetable production, where intensive organic fertilization is applied. the present study examined the effects of dom derived from commercial organic fertilizers (cof), cow manure (com) and chicken manure (chm), on uptake and translocation of lead (pb) in brassica chinensis in a pot experiment. the results indicate that dom derived from chm (domchm) significantly increased pb conce ...201627399753
accumulation of cr, cd, pb, cu, and zn by plants in tanning sludge storage sites: opportunities for contamination bioindication and phytoremediation.tanning sludge enriched with high concentrations of cr and other metals has adverse effects on the environment. plants growing in the metalliferous soils may have the ability to cope with high metal concentrations. this study focuses on potentials of using native plants for bioindication and/or phytoremediation of cr-contaminated sites. in the study, we characterized plants and soils from six tanning sludge storage sites. soil in these sites exhibited toxic levels of cr (averaged 16,492 mg kg(-1 ...201627552994
analysis of genetic diversity of brassica rapa var. chinensis using issr markers and development of scar marker specific for fragrant bok choy, a product of geographic indication.non-heading chinese cabbage [brassica rapa var. chinensis (linnaeus) kitamura] is a popular vegetable and is also used as a medicinal plant in traditional chinese medicine. fragrant bok choy is a unique accession of non-heading chinese cabbage and a product of geographic indication certified by the ministry of agriculture of china, which is noted for its rich aromatic flavor. however, transitional and overlapping morphological traits can make it difficult to distinguish this accession from other ...201627173238
[rna-dependent rna-polymerase in brassica chinensis l]. 20164987649
effect of kelp waste extracts on the growth and development of pakchoi (brassica chinensis l.).to explore the effects of kelp waste extracts (kwe) on the growth and development of brassia chinensis l., germination and greenhouse experiments were carried out under different concentrations of kwe. the results showed that a higher germination percentage (95%), associated with high germination index (8.70), germination energy (71.67%) and seedling vigor index (734.67), was obtained under a lower kwe concentration (2%) compared with the control. the radicle length (4.97 cm), fresh weight (0.32 ...201627934911
comparative transcriptome analysis between low- and high-cadmium-accumulating genotypes of pakchoi (brassica chinensis l.) in response to cadmium stress.to reduce cadmium (cd) pollution of food chains, screening and breeding of low-cd-accumulating cultivars are the focus of much study. two previously identified genotypes, a low-cd-accumulating genotype (lajk) and a high-cd-accumulating genotype (hajs) of pakchoi (brassica chinesis l.), were stressed by cd (12.5 μm) for 0 h (t0), 3 h (t3) and 24 h (t24). by comparative transcriptome analysis for root tissue, 3005 and 4343 differentially expressed genes (degs) were identified in lajk at t3 (vs t0) ...201627228483
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