Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| homogeneous cell suspension of malassezia pachydermatis obtained with an ultrasonic homogenizer. | it is difficult to produce homogeneous cell suspensions of malassezia pachydermatis, since yeast cells paste up and form many clumps. however, homogeneous fungal suspensions are required for susceptibility examinations and biochemical analyses. although several types of trials have been carried out using glass homogenizers and many types of agents to obtain homogeneous fungal suspension. they have not yielded good results. we therefore attempted to use an ultrasonic homogenizer to separate clump ... | 2002 | 12014587 |
| immunoglobulin g responses to malassezia pachydermatis in healthy dogs and dogs with malassezia dermatitis. | serum immunoglobulin g (igg) responses of healthy dogs and dogs with malasseziapachydermatis dermatitis were compared by western immunoblotting. m pachydermatis cbs 1879 was disrupted mechanically and its proteins were separated and blotted on to nitrocellulose membranes before being incubated with sera from eight healthy beagles, eight irish setters with gluten-sensitive enteropathy, 15 healthy basset hounds, and 30 dogs with mpachydermatis-associated dermatitis, 20 of which were basset hounds. ... | 2002 | 12017525 |
| occurrence of malassezia spp. in the external ear canals of dogs and cats with and without otitis externa. | we studied the lipophilic microbiota of the external ear canals of 332 animals (264 dogs and 68 cats), with and without otitis externa, over an 11-year period from 1988 to 1999. malassezia pachydermatis was isolated from 62.2% and 50% of dogs with and without otitis externa, respectively, and from 41.2% and 17.6% of cats with and without otitis externa, respectively. in the group of animals studied for lipid-dependent species, these yeasts were isolated from 4.5% of dogs with otitis externa and ... | 2002 | 12058723 |
| antifungal effect of australian tea tree oil on malassezia pachydermatis isolated from canines suffering from cutaneous skin disease. | the lipophilic yeast malassezia pachydermatis is part of the normal skin flora of most warm-blooded organisms. in a number of surveys it could be demonstrated that this yeast species might be involved in different skin diseases like seborrhoeic dermatitis, especially in dogs and cats. in order to look for an alternative therapeutic agent to the commonly used antimycotic and antiseptic synthetic substances the in vitro activity of australian tea tree oil, the essential oil of melaleuca alternifol ... | 2002 | 12070905 |
| identification of major allergens of malassezia pachydermatis in dogs with atopic dermatitis and malassezia overgrowth. | we have previously shown that both atopic and normal dogs generate an igg response to antigens of malassezia pachydermatis. the aim of this study was to compare ige responses to separated proteins of m. pachydermatis in 28 atopic dogs with malassezia dermatitis and 22 clinically normal dogs using western immunoblotting. six different detection systems were evaluated in order to assess sensitivity and eliminate nonspecific binding and cross-reactivity. the protocol yielding the best results utili ... | 2002 | 12074703 |
| comparison of pulse administration versus once daily administration of itraconazole for the treatment of malassezia pachydermatis dermatitis and otitis in dogs. | objective to compare clinical efficacy of pulse administration with itraconazole versus once daily administration for the treatment of cutaneous and otic m pachydermatis infection in dogs. | 2002 | 12092953 |
| pathological and clinical aspects of the diseases caused by malassezia species. | from veterinary point of view malassezia pachydermatis has the greatest significance. it has been standing in the focus of interest since the early 1990s, mostly because of the frequency of otitis externa and dermatitis caused by this yeast in dogs. this is the only lipid-independent species in the genus malassezia. it can be found in very large proportion on the skin of healthy animals, but can be isolated in much greater number from diseased dogs. it often causes illness together with other pa ... | 2002 | 12109170 |
| apparent cross-infection with a single strain of malassezia pachydermatis on a pig farm. | twenty-nine isolates of malassezia pachydermatis were recovered from a single farm of 100 pigs in croatia. in contrast, 290 farm pigs from other locations (northern parts of croatia and slovenia) yielded only two non-lipid dependent isolates of m. pachydermatis using the same swabbing procedure. ten of the 29 isolates from a single farm had their identity confirmed by karyotyping, and were typed by restriction fragment length polymorphism (rflp) analysis. all but one of these isolates sub-typed ... | 2002 | 12113169 |
| intradermal test reactivity to malassezia pachydermatis in healthy basset hounds and basset hounds with malassezia dermatitis. | nineteen healthy beagles, eight healthy basset hounds and 17 basset hounds with malassezia dermatitis were tested intradermally with two extracts of m pachydermatis. one healthy beagle and two affected basset hounds showed wheal and flare reactions 15 minutes after the injection. delayed reactions, consisting of erythematous macules and plaques, were commonly observed 24 hours after the injection in both the healthy and affected basset hounds, but occurred infrequently in the beagles. at 24 hour ... | 2002 | 12180658 |
| identification of atypical strains of malassezia spp. from cattle and dog. | yeast species in the genus malassezia are lipophilic with the exception of malassezia pachydermatis. during a study of the occurrence of malassezia species in the external ear of 964 cattle and 6 dogs in minas gerais, brazil, six lipid-dependent isolates could not be identified to known species. four isolates came from healthy cows, one from a cow with otitis, and one from a healthy dog. when tested with tweens and cremophor el as single sources of lipids, the strains grew on all sources except ... | 2002 | 12381031 |
| failure of extracts from malassezia pachydermatis to stimulate canine keratinocyte proliferation in vitro. | epidermal hyperplasia is one of the major histopathological features seen in dogs with malassezia dermatitis. the aim of this study was to investigate the effects of extracts and culture supernatants from malassezia pachydermatis on the proliferation of canine keratinocytes. keratinocyte cultures were established from normal dog skin, and cell monolayers were co-cultured with malassezia extracts (prepared either with or without protease inhibitors) and supernatants derived from organisms grown i ... | 2002 | 12464065 |
| evaluation of the effect of ph on in vitro growth of malassezia pachydermatis. | the purpose of this study was to evaluate the effects of ph on the growth of canine malassezia pachydermatis isolates in vitro. yeast growth was monitored by measuring the optical density with a spectrophotometer. the growth of american type culture collection and field strains of m. pachydermatis was optimal between the ph values of 4.0 and 8.0, and inhibited at the ranges of 1.0 to 3.0 and 9.0 to 10.0. an analysis of covariance showed no significant differences among the growth curves at ph le ... | 2003 | 12528830 |
| comparison of two sampling techniques for the detection of malassezia pachydermatis on the skin of dogs with chronic dermatitis. | adhesive tape strip and dry swab sampling techniques were compared for the detection of malassezia pachydermatis on the skin of dogs with chronic dermatitis. one hundred and four dogs were sampled by each of the techniques. two methods, a culture method and a stain method, were used to assess the sampling techniques. by the adhesive tape strip sampling technique, m. pachydermatis was detected on 83 (80%) dogs using the culture method and on 45 (43%) dogs using the stain method. by the dry swab s ... | 2003 | 12573599 |
| efficacy of selamectin administered topically in the treatment of feline otoacariosis. | the efficacy of a novel avermectin, selamectin (stronghold, pfizer), was evaluated against naturally acquired aural infestations of otodectes cynotis. selamectin was administered topically in a single spot to the skin of each animal's back at the base of the neck in front of the scapulae at a minimum dosage of 6mgkg(-1). thirty cats of a cattery of 120 animals with an endemic infestation were treated on days 0 and 30. including untreated control cats was therefore not possible because of animal ... | 2003 | 12591199 |
| evaluation of serum obtained from atopic dogs with dermatitis attributable to malassezia pachydermatis for passive transfer of immediate hypersensitivity to that organism. | to determine the functionality of canine anti-malassezia ige via the passive transfer of immediate hypersensitivity localized to the skin (ie, cutaneous anaphylaxis) from atopic dogs with dermatitis attributable to overgrowth of malassezia pachydermatis (malassezia dermatitis [md]) to healthy recipient dogs by use of the prausnitz-küstner (p-k) technique. | 2003 | 12661863 |
| [etiological factors of fungemia in the hospital san martín in la plata]. | to determine the distribution of etiologic agents of fungemia in san martin hospital, la plata, we retrospectively studied 81 consecutive episodes of fungemia, diagnosed in 46 adults and 35 preterm newborn (pnb) hospitalized from november 1998 to august 2001. the diagnosis was achieved by blood culture obtained by venipuncture and by catheter aspiration and was processed using bactalert and lysis-centrifugation technique. isolated yeasts were identified employing api 32c system and additional te ... | 2003 | 12920994 |
| isolation of malassezia pachydermatis from the skin of captive rhinoceroses. | 2003 | 14567667 | |
| study of the dermatophytes in dogs and the risk of human infection. | the infiltrative growth of fungi that multiplies in the tissues (dermatophytes, candida, agents of visceral mycoses) is the generally known proof of "active" functioning of these organisms. the relative importance of fungi as inducers of human and animal diseases grows constantly. | 2003 | 15168865 |
| assessment of the ability of malassezia pachydermatis to stimulate proliferation of canine keratinocytes in vitro. | to investigate the direct interaction between canine keratinocytes and live malassezia pachydermatis and thereby determine the role of these organisms in the pathogenesis of epidermal hyperplasia associated with malassezia dermatitis in dogs. | 2004 | 15198219 |
| occurrence of malassezia species in healthy and dermatologically diseased dogs. | the presence of malassezia spp. yeasts was investigated in dermatological specimens of 224 dogs, 164 dermatologically diseased and 60 normal dogs. subjects included in the study were of different breed, age, sex and habitat. malassezia spp. positive cultures were obtained in 142 (63.4%) specimens: 67.6% from dermatologically diseased subjects and 51.6% from healthy dogs. malassezia pachydermatis, either as a pure culture or in association with lipid-dependent species, was identified in 138 (97%) ... | 2004 | 15281400 |
| [viability of malassezia pachydermatis strains maintained in various storage mediums]. | the maintenance of malassezia pachydermatis in fungal collections is very important for retrospective and prospective studies. the aim of this study was to evaluate the behavior of malassezia pachydermatis in different storage methods. after the identification process, m. pachydermatis strains were stored for six and nine months, in saline and saline plus mineral oil at 28 degrees c, as well as in dixon's agar, dixon's agar plus glycerol and dixon's agar plus dimethyl-sulfoxide (dmso), at -20 de ... | 2004 | 15330062 |
| association between phospholipase production by malassezia pachydermatis and skin lesions. | an evaluation was made of the phospholipase activities of malassezia pachydermatis strains isolated from healthy dogs versus those from dogs with dermatitis and otitis. a high percentage of strains of m. pachydermatis obtained from lesion sites (93.9%) produced phospholipase, compared to the strains obtained from healthy skin of the same dog with localized lesions (41.4%) and healthy dogs (10.6%). | 2004 | 15472366 |
| seborrhoeic dermatitis in a goat due to malassezia pachydermatis. | a 6-year-old female goat was presented with a seborrhoeic dermatosis of 5 months duration. the condition developed following a severe enteritis associated with weight loss. dermatological examination showed a generalized greasy seborrhoeic dermatosis, which spared the head and extremities of the limbs. microscopic examination of impression smears of lesional skin revealed numerous yeasts typical of malassezia sp. culture on sabouraud's dextrose agar yielded malassezia pachydermatis growth. histo ... | 2004 | 14989707 |
| clinical, histopathological and immunological effects of exposure of canine skin to malassezia pachydermatis. | the effects of the daily application for 7 days of suspensions of malassezia pachydermatis to normal canine skin were evaluated in 10 beagle dogs. four out of six dogs challenged without occlusion developed transient lesions generally characterized clinically by mild erythema with papules and histologically by mild epidermal hyperplasia and a superficial perivascular dermatitis. saline-treated control sites showed no clinical signs. in four dogs challenged with occlusion, skin lesions occurred a ... | 2004 | 15124870 |
| higher incidence of malassezia pachydermatis in the eyes of dogs with corneal ulcer than in healthy dogs. | malassezia pachydermatis is usually associated with otitis and dermatitis in dogs but it can also cause diseases in other species, including humans. in a human neonatal intensive care unit, m. pachydermatis was isolated from an infant's ocular discharge. therefore, the aim of this study was to ascertain the presence of malassezia spp. and its possible consequences in dogs' eyes. this research included 19 dogs with unilateral or bilateral corneal ulcers and 60 healthy dogs. a total of 158 clinica ... | 2004 | 15135519 |
| malassezia pachydermatis carriage in dog owners. | yeasts of the genus malassezia serve as both commensal microorganisms and pathogens on the skin of humans and domestic animals. although rare, cases of life-threatening fungemia in people have been attributed to malassezia pachydermatis, for which dogs are a natural host. zoonotic transfer has been documented from dogs to immunocompromised patients by healthcare workers who own dogs. we investigated the role of pet dogs as risk factors for mechanical carriage of m. pachydermatis on human hands. ... | 2005 | 15705327 |
| dna sequence diversity of intergenic spacer i region in the non-lipid-dependent species malassezia pachydermatis isolated from animals. | the non-lipid-dependent species malassezia pachydermatis is frequently isolated from animals. we analyzed the dna sequences of the intergenic spacer (igs) 1 region, which is the most variable region in the rrna gene, of 43 m. pachydermatis strains obtained from dogs or cats. the lengths of the igs 1 regions ranged from 552 to 898 bp and, based on the nucleotide sequence, these igs 1 regions were divided into three major groups with 10 subtypes. group 1 (552-601 bp long) was characterized by the ... | 2005 | 15712605 |
| isolation of malassezia species from healthy cats and cats with otitis. | lipid-dependent malassezia species have recently been cultured from veterinary specimens. the identification of malassezia species isolates from animals is important to clarify the epidemiology of these lipophilic yeasts. malassezia species were cultured from the external ear canals of 63 out of 99 cats with otitis and 12 of 52 (23%) healthy control cats. the rate of isolation in affected animals versus controls was highly significant (p<0.01). malassezia pachydermatis was isolated as a pure cul ... | 2005 | 15922220 |
| genetic typing of malassezia pachydermatis from different domestic animals. | in the present study, random amplification of polymorphic dna, which detects dna polymorphism in fungal genomic dna, was applied for genetic typing of malassezia pachydermatis isolates. fifty-five isolates from different domestic animals and body sites and the neotype strain cbs 1879 were characterized. primers m13 and opt-20 were used to analyse their genetic relatedness and similarity. this technique allowed us to distinguish four different genetic types. the predominant genetic type was obser ... | 2005 | 15922521 |
| canine serum immunoreactivity to m. pachydermatis in vitro is influenced by the phase of yeast growth. | in western immunoblotting studies of canine sera using malassezia pachydermatis extracts, the reported patterns of immunoreactivity vary between different laboratories. because the duration of culture influences the antigenic composition of lipid-dependent malassezia spp. when probed with human sera, we investigated whether the in vitro growth phase of m. pachydermatis influences immunoreactivity using canine sera. extracts of m. pachydermatis cbs 1879 grown in sabouraud's liquid medium at 37 de ... | 2005 | 15960626 |
| humoral measurement of type-1 hypersensitivity reactions to a commercial malassezia allergen. | malassezia pachydermatis is considered to be a contributing factor to canine atopic dermatitis (ad). the purpose of this study was to investigate the humoral response to a commercially produced m. pachydermatis extract. fifteen atopic dogs with malassezia overgrowth on the skin (md), 16 atopic dogs without md, three atopic dogs with overgrowth of malassezia in the ears only (mo), and 12 normal dogs were intradermally tested with m. pachydermatis extract at 50, 100, 250, 500, 1000, 2000 and 4000 ... | 2005 | 16101797 |
| frequency, body distribution, and population size of malassezia species in healthy dogs and in dogs with localized cutaneous lesions. | malassezia species are commensal organisms of human and animal skin that occasionally act as opportunistic pathogens. the lipid-dependent species are associated with human skin disorders, whereas the non-lipid-dependent species (malassezia pachydermatis) is considered as an opportunistic secondary pathogen affecting the canine skin surface and ear canal. this study evaluated the relationship between malassezia yeasts, their population size, and the occurrence of skin lesions from healthy and ski ... | 2005 | 16130988 |
| bovine and canine transferrin inhibit the growth of malassezia pachydermatis in vitro. | transferrin, an iron-binding beta-globulin protein that transports iron to mammalian cells, may contribute to innate immunity to fungal pathogens, primarily by limiting microbial access to iron. we investigated whether unsaturated (apo) canine and bovine transferrin had an inhibitory effect in vitro on malassezia pachydermatis, an important opportunistic cutaneous yeast pathogen of dogs. m. pachydermatis strains were grown at 32 degrees c in 96-well culture plates using sabouraud's liquid medium ... | 2005 | 16178374 |
| effects of beta-thujaplicin on anti-malassezia pachydermatis remedy for canine otitis externa. | the antifungal activity of beta-thujaplicin was evaluated against 51 malassezia pachydermatis strains isolated from canine ear canals with or without otitis externa. for comparison, sensitivity tests were performed on m. pachydermatis isolates for nystatin, ketoconazole, and terbinafine hcl, all clinically available antifungal agents. the minimal inhibition concentrations over 50% of the tested isolates (mic50) were 3.13 microg/ml for beta-thujaplicin and nystatin, 0.016 microg/ml for ketoconazo ... | 2005 | 16397383 |
| production of antihypertensive angiotensin i-converting enzyme inhibitor from malassezia pachydermatis g-14. | to produce a novel antihypertensive angiotensin i-converting enzyme (ace) inhibitor from yeast, a yeast isolate, designated g-14 showing the highest ace inhibitory activity was obtained and identified as malassezia pachydermatis based on morphological, biochemical and cultural characteristics. the maximal extracellular ace inhibitor production was obtained from m. pachydermatis g-14 when the strain was cultured in yepd medium containing 0.5% yeast extract, 3.0% peptone and 2.0% glucose at 30℃ fo ... | 2005 | 24049490 |
| distribution of malassezia organisms on the skin of unaffected psittacine birds and psittacine birds with feather-destructive behavior. | to ascertain whether malassezia organisms can be detected via cytologic examination and fungal culture of samples from the skin surface of psittacine birds and determine whether the number of those organisms differs between unaffected psittacines and those that have chronic feather-destructive behavior or differs by body region. | 2006 | 16426189 |
| prevalence of zoonotic agents in dogs visiting hospitalized people in ontario: implications for infection control. | visitation of hospitalized people by dogs is becoming commonplace, but little is known about the potential health risks of introducing dogs to healthcare settings. this cross-sectional study evaluated the prevalence of zoonotic agents in a group of 102 visitation dogs from a variety of sources across ontario. between may and july 2004, owners were interviewed by a standardized questionnaire while dogs underwent a standardized physical examination. one specimen of faeces, hair-coat brushings and ... | 2006 | 16466831 |
| rapid identification of fungal pathogens in bact/alert, bactec, and bbl mgit media using polymerase chain reaction and dna sequencing of the internal transcribed spacer regions. | we report a direct polymerase chain reaction/sequence (d-pcrs)-based method for the rapid identification of clinically significant fungi from 5 different types of commercial broth enrichment media inoculated with clinical specimens. media including bact/alert fa (biomérieux, marcy l'etoile, france) (n = 87), bactec plus aerobic/f (becton dickinson, microbiology systems, sparks, md) (n = 16), bactec peds plus/f (becton dickinson) (n = 15), bactec lytic/10 anaerobic/f (becton dickinson) (n = 11) b ... | 2006 | 16466900 |
| isolation, characterization and molecular cloning of a lipolytic enzyme secreted from malassezia pachydermatis. | lipophilic malassezia species may induce catheter-associated sepsis in premature neonates and immunocompromised patients receiving parenteral lipid emulsions. to assess the participation of lipolytic enzymes in the pathogenesis of this yeast, we cloned a gene encoding the enzyme. a lipolytic enzyme in the culture supernatant of malassezia pachydermatis was purified 210-fold to homogeneity. the enzyme showed high esterase activity toward p-nitrophenyl octanoate. the cdna encoding the enzyme was c ... | 2006 | 16487331 |
| patch test responses to malassezia pachydermatis in healthy dogs. | the effects of the patch test application of malassezia pachydermatis extracts to normal canine skin were evaluated in eight healthy beagle dogs. antigens (4 and 0.4 mg/ml) and saline controls were applied for 48 h using filter paper discs in finn chambers. at the first test, two dogs showed patch test reactivity 20 min and 24 h after patch removal. four out of six dogs that did not react to the first patch test showed reactivity when re-tested on day 8. two remaining dogs were patch tested for ... | 2006 | 16519021 |
| isolation of malassezia spp. from cerumen of wild felids. | the objective of this study was to determine the presence of different species of the genus malassezia in the healthy external auditory canal of wild felids maintained in captivity. one hundred and thirty-two adult animals (264 samples of cerumen), 77 males (58.3%) and 55 females (41.7%), were studied: large felids (55 animals) - 26 lions (panthera leo), 13 tigers (panthera tigris), 6 leopards (panthera pardus), 6 jaguars (panthera onca), 2 cheetahs (acinonyx jubatus), 2 pumas (puma concolor); s ... | 2006 | 16772235 |
| patch test responses to malassezia pachydermatis in healthy basset hounds and in basset hounds with malassezia dermatitis. | the effects of the patch test application of malassezia pachydermatis extracts were evaluated in seven healthy basset hounds and in seven basset hounds with malassezia dermatitis. antigens (4 and 0.4 mg/ml) and saline controls were applied for 48 h using filter paper discs in finn chambers. one healthy basset hound and five affected hounds showed positive patch test reactivity to the yeast antigens. positive patch test reactions were characterized histologically by mild epidermal hyperplasia and ... | 2006 | 16882608 |
| mycologic disorders of the skin. | cutaneous tissue can become infected when fungal organisms contaminate or colonize the epidermal surface or hair follicles. the skin can be a portal of entry for fungal infection when the epithelial barrier is breached or it can be a site for disseminated, systemic fungal disease. the two most common cutaneous fungal infections in small animals are dermatophytosis and malassezia dermatitis. dermatophytosis is a superficial cutaneous infection with one or more of the fungal species in the keratin ... | 2006 | 16933479 |
| granulomatous skin infection caused by malassezia pachydermatis in a dog owner. | malassezia pachydermatis is part of the normal cutaneous microflora of dogs and many other mammals. m pachydermatis has not yet been reported as an agent that causes skin infection in humans, although it has been found to cause fungemia and other nosocomial infections in preterm newborns and immunocompromised adults. | 2006 | 16983005 |
| malassezia pachydermatis isolated from normal and diseased external ear canals in dogs: a comparative analysis. | to investigate the role of malassezia pachydermatis as a pathogenic agent in canine otitis, a comparative analysis of isolates from normal and diseased external ear canals in dogs was undertaken. specimens were collected from the ears of dogs with unilateral or bilateral otitis and from healthy dogs. mycological analysis was by direct microscopy and fungal culture on sabouraud's dextrose agar and dixon's agar. of the otitis specimens, 63.7% showed typical malassezia cells on cytological examinat ... | 2006 | 16154787 |
| [rapd differentiation of yeast like fungi malassezia pachydermatis]. | the aim of the work was analyzing of genomic dna of malassezia pachydermatis isolates from clinical cases otitis externa from dogs using rapd method with arbitrary primers eric 1r, eric2, bg2 and fm1. materials and methods: 47 strains of m. pachydermatis isolates from clinical cases otitis externa from dogs were tested. isolation of genomic dna was provided according with masterpuretm yeast dna purification kit epicentre procedure. the quality of isolated genomic dna was determined electrophoret ... | 2006 | 17432622 |
| treatment of malassezia pachydermatis-associated seborrhoeic dermatitis in devon rex cats with itraconazole--a pilot study. | treatment of malassezia pachydermatis-associated seborrhoeic dermatitis with oral itraconazole was investigated in six devon rex cats (drc). the cutaneous populations of malassezia were determined using contact plates and a swab-wash method before and after 21 days of pulse treatment with itraconazole (5 mg kg-1 once daily, 7 days on, 7 days off, 7 days on). before treatment, all cats had greasy seborrhoeic dermatitis involving the axillae, groin, claw folds and palmar and plantar interdigital s ... | 2007 | 17470232 |
| biofilm development by clinical isolates of malassezia pachydermatis. | malassezia pachydermatis fungemia has been reported in patients receiving parenteral nutrition. biofilm formation on catheters may be related to the pathogenesis of this mycosis. we investigated the biofilm-forming ability of 12 m. pachydermatis strains using a metabolic activity plate-based model and electronic microscopic evaluation of catheter surfaces. all m. pachydermatis strains developed biofilms but biofilm formation showed variability among the different strains unrelated to their clini ... | 2007 | 17510859 |
| prevalence of malassezia spp. yeasts in feline nail folds: a cytological and mycological study. | malassezia spp. yeasts are commensal organisms of mammal and avian skin, but little is known about their presence on the skin of healthy cats. the purposes of this study were to evaluate the prevalence of malassezia spp. yeasts in feline nail folds and to identify the different species. forty-six cats of different breeds were evaluated by cytological examination, and malassezia spp. yeasts were seen in 61% of them. yeasts were found in 100% of devon rex cats [mean 8.63/oil immersion field (high- ... | 2007 | 17610495 |
| malassezia spp. overgrowth in allergic cats. | a series of 18 allergic cats with multifocal malassezia spp. overgrowth is reported: atopic dermatitis was diagnosed in 16, an adverse food reaction in another and one was euthanized 2 months after diagnosis of malassezia overgrowth. all the cats were otherwise healthy and those tested (16 out of 18) for feline leukaemia or feline immunodeficiency virus infections were all negative. at dermatological examination, multifocal alopecia, erythema, crusting and greasy adherent brownish scales were va ... | 2007 | 17845619 |
| identification and antimicrobial susceptibility of bacteria and yeasts isolated from healthy dogs and dogs with otitis externa. | the bacterial and fungal flora of the external ear canal of dogs with otitis externa and of healthy dogs were studied. the most frequently isolated microorganism from otitic ears was staphylococcus intermedius (58.8%), followed by malassezia pachydermatis (30.9%), streptococcus canis (29.9%), proteus spp. (14.4%) and escherichia coli (10.3%). a statistical analysis of our results showed that the prevalence of these microorganisms is significant in dogs with otitis externa. furthermore, the antim ... | 2007 | 18045339 |
| isolation of malassezia pachydermatis and m. sympodialis from the external ear canal of cats with and without otitis externa. | the objective of this study was to determine the presence of malassezia spp. in the external ear canal of cats with and without otitis. forty-five animals were studied, 20 with and 25 without otitis externa (oe). cerumen or secretion from external ear canal samples was cultured on modified mycosel agar and sterile olive oil was added to the surface of the medium before specimen seeding. the isolates were analysed for macro- and micromorphology and identified by catalase tests and on the basis of ... | 2007 | 18277706 |
| microtubular and actin cytoskeletons and ultrastructural characteristics of the potentially pathogenic basidiomycetous yeast malassezia pachydermatis. | microtubular and actin cytoskeletons were investigated in the lipophilic yeast malassezia pachydermatis by fluorescence and electron microscopy. to detect microtubules by indirect immunofluorescence using monoclonal anti-tubulin antibody, a prolonged incubation with lysing enzymes was necessary due to its very thick cell wall. cytoplasmic microtubules were detected in interphase and a spindle with astral microtubules was seen in m-phase. the disintegration of cytoplasmic microtubules and migrati ... | 2007 | 17055753 |
| phenotypic characterization and in vitro antifungal sensitivity of candida spp. and malassezia pachydermatis strains from dogs. | yeasts of the genera candida and malassezia can be found as commensal microorganisms in animals. the main species of importance in veterinary medicine are malassezia pachydermatis and candida albicans. the objectives of this study were to conduct a phenotypic characterization and to evaluate the in vitro antifungal sensitivity of strains of c. albicans (n=5), c. tropicalis (n=3) and m. pachydermatis (n=32) isolated from dogs. the phenotyping was based on macro and micromorphological features as ... | 2007 | 17188535 |
| in vitro anti-malassezia activity of xanthorrhizol isolated from curcuma xanthorrhiza roxb. | this study aimed at investigating the anti-malassezia activity of xanthorrhizol (xtz) isolated from curcuma xanthorrhiza roxb. against malassezia furfur atcc 14521 and malassezia pachydermatis atcc 14522. | 2007 | 17257249 |
| occurrence, distribution and population size of malassezia pachydermatis on skin and mucosae of atopic dogs. | aim of the present study was to determine the distribution and quantification of malassezia yeasts on a wide number of cutaneous sites in atopic dogs by means of a semiquantitative swab technique. a possible relationship between the presence of clinical signs and the occurrence and population size of yeasts was attempted. forty-one privately owned atopic dogs of different age and breed were sampled. results were expressed as colony forming units per swab. malassezia colonies obtained from each p ... | 2007 | 17257784 |
| molecular characterization of malassezia isolates from dogs using three distinct genetic markers in nuclear dna. | little precise information is available on the systematics, genetics, ecology and epidemiology of yeasts of the genus malassezia from different animal species. in the present study, one hundred and four isolates of malassezia (lipid dependent or non-lipid dependent) from dogs were characterized by their chitin synthase 2 gene (chs2), and the large subunit (lsu) and the first internal transcribed spacer (its-1) of nuclear ribosomal dna sequences, and compared genetically with well-defined referen ... | 2007 | 17320347 |
| role of beta-endorphin on phospholipase production in malassezia pachydermatis in dogs: new insights into the pathogenesis of this yeast. | malassezia spp. are lipophilic yeasts that are part of the normal cutaneous microflora and sometimes act as pathogens causing dermatitis. this study investigated the interactions occurring between beta-endorphin and phospholipase activity in isolates of m. pachydermatis in dogs presenting cutaneous lesions. phospholipase production was evaluated and quantified on 144 isolates suspended in dixon broth to which different beta-endorphin concentrations (from 600 to 0.6 pm) were added. the isolates w ... | 2007 | 17325939 |
| genetic and biochemical characterization of malassezia pachydermatis with particular attention to pigment-producing subgroups. | the aim of the study was the characterization of malassezia pachydermatis and its pigment-producing subgroup using biochemical tests and rapd. it was of interest to determine whether particular rapd patterns could be used to indicate pigment production, as well as a close genetic relatedness to malassezia furfur. therefore, 210 strains of m. pachydermatis were examined for morphology, catalase and ss-glucosidase activity, lipid and carbohydrate assimilation and the tryptophan-dependent synthesis ... | 2007 | 17325943 |
| in vitro activity of an ear rinse containing tromethamine, edta, benzyl alcohol and 0.1% ketoconazole on malassezia organisms from dogs with otitis externa. | the purpose of this study was to evaluate the in vitro activity of an ear rinse containing tromethamine, edta, benzyl alcohol and 0.1% ketoconazole in purified water on malassezia organisms from dogs with otitis externa. malassezia organisms were collected from ear swab samples from the external ear canal of 19 dogs with otitis externa plus one control strain of malassezia pachydermatis. three test solutions were evaluated: er (edta, tromethamine, benzyl alcohol), er + keto (edta, tromethamine, ... | 2007 | 17355426 |
| in vitro antimicrobial activity of the leaf extract of harungana madagascariensis lam. ex poir. (hypericaceae) against strains causing otitis externa in dogs and cats. | otitis externa in dogs and cats is always caused by a combination of yeasts and bacteria, among which the most important are malassezia pachydermatis, staphylococcus intermedius and pseudomonas species. these organisms often develop resistance to classical antimicrobial agents. therefore, the aim of this study was to investigate the antimicrobial activities of an ethyl acetate leaf extract of harungana madagascariensis against the organisms cited, to carry out the phytochemical investigation of ... | 2007 | 17385560 |
| genetic variants of malassezia pachydermatis from canine skin: body distribution and phospholipase activity. | malassezia pachydermatis isolates (n=185) from skin sites from dogs (n=30) were characterized genetically and biochemically following in vitro culture. two regions in the chitin synthase-2 gene (chs-2) and the first internal transcribed spacer (its-1) of nuclear ribosomal dna were sequenced, and the phospholipase activity of each isolate was assessed. three chs-2 (i.e. ac, bc and cc) and eight its-1 (i.e. ai1, ai2, ai3, ai4, bi1, ci1, ci2 and ci3) sequence types were defined for all 185 samples. ... | 2008 | 18294200 |
| frequency of yeasts and dermatophytes from healthy and diseased dogs. | the aim of this study was to investigate the presence of dermatophytes and yeasts in healthy and diseased dogs. a total of 633 samples were collected from 26 healthy animals (104 samples), 131 with dermatitis (343 samples), 74 with otitis (148 samples), and 19 with ocular diseases (38 samples). cultures from healthy animals were positive for malassezia pachydermatis in 13.5% (7/52) of samples from skin, 42.3% (11/26) from ear, and 3.8% (1/26) from eye. fungal growth was observed in 20.4% (70/343 ... | 2008 | 18319432 |
| immune response to fungal infections. | the immune mechanisms of defence against fungal infections are numerous, and range from protective mechanisms that were present early in evolution (innate immunity) to sophisticated adaptive mechanisms that are induced specifically during infection and disease (adaptive immunity). the first-line innate mechanism is the presence of physical barriers in the form of skin and mucous membranes, which is complemented by cell membranes, cellular receptors and humoral factors. there has been a debate ab ... | 2008 | 18565595 |
| subculture on potato dextrose agar as a complement to the broth microdilution assay for malassezia pachydermatis. | the main aim of this study was to verify the efficacy of subculture on potato dextrose agar (pda) as a complement to the in vitro susceptibility test for malassezia pachydermatis strains by a broth microdilution method, as well as to determine the mic and mfc of azole derivatives, amphotericin b and caspofungin. the microdilution assay was performed in 96-well plates using a modified rpmi 1640 medium. the m. pachydermatis strains were resistant to caspofungin. all strains (n=50) had shown mic va ... | 2008 | 18603321 |
| the pathogenesis of malassezia yeasts. | the genus malassezia includes twelve species of yeast, many of which have been mainly associated with human and canine diseases. malassezia pachydermatis colonizes the skin and mucosal sites of healthy dogs and cats. despite being part of the normal cutaneous microflora, malassezia spp. yeast may become pathogenic under certain circumstances. this article reviews the factors related to both host and yeast which affect the pathogenical or commensal phenotypes of malassezia yeasts. | 2008 | 18693560 |
| comparative study of the activity and kinetic properties of malate dehydrogenase and pyruvate decarboxylase from candida albicans, malassezia pachydermatis, and saccharomyces cerevisiae. | candida albicans and malassezia pachydermatis cause human and animal infections of the skin and internal organs. we compare the properties of two enzymes, pyruvate decarboxylase (pdc) and malate dehydrogenase (mdh), from these species and from saccharomyces cerevisiae cultivated under aerobic and anaerobic conditions to find differences between the enzymes that adapt pathogens for virulence and help us in searching for new antifungal agents. malassezia pachydermatis did not show any growth under ... | 2008 | 18772936 |
| comparative study of the microbial profile from bilateral canine otitis externa. | fifty dogs with bilateral otitis externa were studied over a 10-month period. the exudates of both external ears were obtained, using sterile swabs, and microorganisms were isolated according to standard microbiological techniques. antimicrobial susceptibility testing of staphylococcus intermedius was done by the agar diffusion method. there was bacterial and/or fungal growth in all of the samples. these were all polymicrobial infections. anaerobic bacteria were not isolated in any sample. the m ... | 2008 | 18978972 |
| comparative in vitro antimicrobial efficacy of commercial ear cleaners. | the aim of this study was to compare the antimicrobial efficacy of ear cleaners against staphylococcus intermedius, pseudomonas aeruginosa and malassezia pachydermatis. single isolates of each organism were incubated in duplicate at 38 degrees c for 30 min with each ear cleaner diluted 1/2 to 1/256 in phosphate-buffered saline. positive and negative controls were included. aliquots were then incubated for 16-18 h on sheep blood agar (bacteria) or for 3 days on sabouraud's dextrose agar (malassez ... | 2008 | 19055612 |
| synergistic effects of miconazole and polymyxin b on microbial pathogens. | the therapeutic value of antibiotics depends on the susceptibility of the infecting microorganism and the pharmacological profile of the drugs. to assess the value of an antibiotic combination of polymyxin b and miconazole this study examined the in vitro synergistic potential of the two drugs on gram-negative and gram-positive bacteria and yeast. antifungal and antibacterial activity was tested by minimum inhibitory concentration (mic) of broth macrodilution and urea broth microdilution, by flu ... | 2008 | 19085068 |
| the anatomical distribution and antimicrobial susceptibility of yeast species isolated from healthy dogs. | the aim of this work was to identify the predominant yeast species present at different anatomical sites in healthy dogs and to determine their in vitro antimicrobial susceptibility using a broth microdilution assay. samples were collected from the preputial, vaginal, oral and perianal mucosae and the isolates cultured were identified according to their morphological characteristics and biochemical profile. malassezia pachydermatis was the most commonly isolated yeast, followed by candida paraps ... | 2009 | 19109040 |
| clsi broth microdilution method for testing susceptibility of malassezia pachydermatis to thiabendazole. | thiabendazole, classified as antiparasitic and also used as an antifungal drug, can be found as otological solution indicated for treatment of parasitic and fungal external otitis in small animals. malassezia pachydermatis is a yeast recognized as a normal inhabitant on the skin and mucous membranes of dogs and cats. however, it is considered an opportunistic agent that causes external otitis and dermatitis in these animals. the aim of this study was to evaluate the in vitro effect of thiabendaz ... | 2009 | 24031347 |
| the cell wall galactomannan antigen from malassezia furfur and malassezia pachydermatis contains beta-1,6-linked linear galactofuranosyl residues and its detection has diagnostic potential. | lipophilic yeasts of the genus malassezia are associated with several skin diseases, such as pityriasis versicolor, malassezia folliculitis, seborrhoeic dermatitis and atopic dermatitis, and are also increasingly associated with catheter-related fungaemia. the cell wall components of pathogenic micro-organisms behave as an antigen and/or ligand of the innate immune response. live cells of malassezia furfur and malassezia pachydermatis did not react with an anti-alpha-1,2-mannoside antibody. howe ... | 2009 | 19389777 |
| chemical composition, toxicity and larvicidal and antifungal activities of persea americana (avocado) seed extracts. | the present study had the aim of testing the hexane and methanol extracts of avocado seeds, in order to determine their toxicity towards artemia salina, evaluate their larvicidal activity towards aedes aegypti and investigate their in vitro antifungal potential against strains of candida spp, cryptococcus neoformans and malassezia pachydermatis through the microdilution technique. in toxicity tests on artemia salina, the hexane and methanol extracts from avocado seeds showed lc50 values of 2.37 ... | 2009 | 19448924 |
| cutaneous carriage of malassezia species in healthy and seborrhoeic sphynx cats and a comparison to carriage in devon rex cats. | cutaneous carriage of malassezia species yeast was investigated in 32 sphynx cats, and in 10 domestic shorthair (dsh) cats. samples for mycological culture were taken using contact plates and swabs at seven sites in each cat (left and right axillae and groin, left ear, claw fold on left front paw and the interdigital palmar web of the left front paw). malassezia species were isolated from 26/32 sphynx cats (81%) and from 0/10 dsh control cats. in five cases malassezia species yeasts were isolate ... | 2009 | 19559635 |
| altered cutaneous expression of beta-defensins in dogs with atopic dermatitis. | canine atopic dermatitis (ad) is a chronic allergic skin disorder with an immunopathogenesis comparable to that in humans with ad. the high frequency of recurrent infections with staphylococcus pseudo intermedius and malassezia pachydermatis may indicate a defective innate immune response in the skin of atopic dogs. production of beta-defensins constitutes an important role in skin defense but information on canine beta-defensin localization and regulation is scarce. we conducted a gene-expressi ... | 2009 | 19576634 |
| efficacy of amitraz plus metaflumizone for the treatment of canine demodicosis associated with malassezia pachydermatis. | abstract: this case reports the efficacy of metaflumizone plus amitraz spot-on formulation (promeris duo(r); fort dodge) against generalized demodectic mange. a two year-old male dog presented at clinical examination with poor general condition, diffused alopecia, crusted lesions, pruritus, skin scales and pustules. demodex mites, malassezia pachydermatis yeasts and bacteria were diagnosed. the dog was treated with cephalexin and topically with metaflumizone plus amitraz spot on formulation at t ... | 2009 | 19265536 |
| identification of malassezia pachydermatis from healthy and diseased human skin. | malassezia pachydermatis is the only species in the genus malassezia that is classically considered to be zoophilic. this yeast is only occasionally isolated from human skin, although it has been found to cause septic epidemics, especially in neonates. the aim of our study was to investigate the prevalence of m. pachydermatis on the skin of patients with malassezia-associated diseases and of healthy subjects. one hundred and sixty skin scrapings from patients with pityriasis versicolor (pv), seb ... | 2009 | 20380109 |
| coryneform bacteria associated with canine otitis externa. | this study aims to investigate the occurrence of coryneform bacteria in canine otitis externa. a combined case series and case-control study was carried out to improve the current knowledge on frequency and clinical significance of coryneform bacteria in samples from canine otitis externa. a total of 16 cases of otitis externa with involvement of coryneform bacteria were recorded at two referral veterinary hospitals in denmark and the us, respectively. coryneform bacteria were identified by part ... | 2010 | 20434850 |
| rapd differentiation of malassezia spp. from cattle, dogs and humans. | the molecular characterization of malassezia spp. isolates from animals and humans has not been thoroughly studied. we have analysed the dna profile by random amplified polymorphic dna (rapd)-pcr to compare the genetic diversity between isolates from the external ears of cattle, dogs and humans. the analysis of electrophoretic profiles on 8% polyacrylamide gel and their phenograms showed genetic heterogeneity between rapd profiles of malassezia furfur and malassezia slooffiae isolates from human ... | 2010 | 19298360 |
| isolation and characterization of malassezia spp. in healthy swine of different breeds. | malassezia spp. genus is represented by several lipophilic yeasts, normally present on the skin of many warm-blooded vertebrates, including man. swine are one of the less investigated animal species. the aim of the present work was to study the occurrence of malassezia spp. in the external ear canal of 408 healthy swine of different breeds, under different breeding conditions. for this purpose n. 185 free-ranging wild boars, n. 107 large size pigs and 116 cinta senese breed were selected. animal ... | 2010 | 19709823 |
| malassezia pachydermatis and m nana predominate amongst the cutaneous mycobiota of sphynx cats. | carriage of malassezia species yeasts in healthy sphynx cats was compared with that in devon rex cats (drc), cornish rex cats (crc) and domestic shorthair (dsh) cats. swab samples from the external ear, anus and claw folds, and contact plate samples from the axillae and groins, were incubated on modified dixon's agar at 32°c for 7 days. malassezia species were isolated from all 18 sphynx cats; m pachydermatis accounted for 118/140 isolates. of 20 isolates of m nana, 16 were recovered from the ea ... | 2010 | 20863732 |
| evaluation of fungal flora in normal and diseased canine ears. | this study was undertaken to characterize otic fungal flora encountered in normal dogs, atopic dogs with no clinical or cytological evidence of otitis and dogs with otitis externa. forty-two normal dogs, 23 atopic dogs and 32 dogs with otitis were included in the study. samples for otic fungal culture and cytology were obtained from all animals, for a total of 194 ears. sixty-seven ear samples (34%) were culture positive for saprophytic fungal organisms, as follows: 43 (64%) penicillium species, ... | 2010 | 20868397 |
| comparative analysis of the frequency, distribution and population sizes of yeasts associated with canine seborrheic dermatitis and healthy skin. | the purpose of this study was to investigate the diversity of yeast associated with the degree of canine seborrheic dermatitis (sd) by anatomical sites. fifty-seven samples were divided as 17 healthy skin, 20 with primary seborrheic dermatitis (psd), and 20 with secondary seborrheic dermatitis (ssd). yeast isolation and characterization were carried out based on microscopical features and biochemical properties. dna analysis at the internal transcribed spacer i of 26s rdna region was utilized fo ... | 2010 | 20961712 |
| the immunoglobulin g response to malassezia pachydermatis extracts in atopic and non-atopic dogs. | igg immunoreactivity to malassezia pachydermatis was compared in atopic and non-atopic dogs. malassezia pachydermatis proteins with a molecular weight of 98 kda were recognized at a significantly higher frequency in the sera of atopic dogs. most of the atopic dogs with malassezia dermatitis had a greater igg response than did normal dogs. | 2010 | 21037887 |
| physiological and molecular characterization of atypical lipid-dependent malassezia yeasts from a dog with skin lesions: adaptation to a new host? | three lipid-dependent malassezia isolates (here named 114a, 114b and 114c) recovered from a dog with skin lesions were phenotypically and genotypically characterized. all presented ovoid cells and buds formed on a narrow base. most of the results from physiological tests were consistent with those of malassezia furfur. the phylogenetic analysis of its-1 and lsu nucleotide sequences was concordant in placing all three clinical malassezia isolates close to m. furfur. however, the phylogenetic data ... | 2010 | 21070187 |
| an azole-resistant isolate of malassezia pachydermatis. | canine malassezia dermatitis (md) is frequently treated with systemic ketoconazole (ktz) and itaconazole (itz). however, the antifungal susceptibility of clinical isolates of m. pachydermatis from dogs and cats to the azoles has not been well investigated. in the present study, the in vitro susceptibility of the standard strain (cbs1879: the neotype strain of m. pachydermatis) and 29 clinical isolates of m. pachydermatis to the azoles was measured by a modified clsi m27-a2 test using modified di ... | 2010 | 21074337 |
| expression of the micro-opioid receptor on malassezia pachydermatis and its effect in modulating phospholipase production. | malassezia spp. may act as opportunistic skin pathogens in humans and animals. malassezia pachydermatis proliferation and phospholipase production may play a pathogenic role in the occurrence of skin lesions in dogs. this study investigates the presence of mu-opioid receptor (mor) in m. pachydermatis strains isolated from healthy dogs and dogs with skin lesions and its effects on phospholipase activity (p.a.). p.a. of 64 m. pachydermatis isolates was evaluated using different concentrations of n ... | 2010 | 19225979 |
| in vitro cytokine responses of peripheral blood mononuclear cells from healthy dogs to distemper virus, malassezia and toxocara. | naïve cd4+ t cells may differentiate into a number of subsets including t helper 1 (th1) th2, th3, th17 and t regulatory (treg) cells depending on the type of antigen they encounter. these cd4+ families have been defined based on the array of cytokines they produce and the effects they have on adaptive immune responses. cd4+ subsets are cross regulatory and at times cooperative. the study of these adaptive immune modulators has revealed the important role that cytokines play in mounting effectiv ... | 2010 | 19880197 |
| antimicrobial activity of propolis extract against staphylococcus coagulase positive and malassezia pachydermatis of canine otitis. | the aims of this study were to evaluate the antimicrobial potential of propolis extract by determining the minimum bactericidal concentration (mbc) for coagulase-positive staphylococcus isolates (staphylococcus aureus and staphylococcus intermedius) and the minimum fungicidal concentration (mfc) for malassezia pachydermatis isolates. the microorganisms were assayed using broth microdilution techniques. the mbc(90) was 21 mg ml(-1), and the mfc(90) was 5.3 mg ml(-1). the propolis extract was foun ... | 2010 | 19913365 |
| in vitro antimicrobial activity of a commercial ear antiseptic containing chlorhexidine and tris-edta. | minimum bactericidal concentrations (mbcs) of a commercial ear antiseptic containing chlorhexidine 0.15% and tris-edta (otodine) were determined by broth microdilution for 150 isolates representing the most common pathogens associated with canine otitis. the microorganisms were classified into three groups according to their levels of susceptibility. the most susceptible group included staphylococcus pseudintermedius, malassezia pachydermatis, streptococcus canis and corynebacterium auriscanis, ... | 2010 | 20030799 |
| genetic variability and phospholipase production of malassezia pachydermatis isolated from dogs with diverse grades of skin lesions. | little detailed information is available on the association of malassezia pachydermatis genotypes and the extent of skin damage that they cause. in the present study, isolates of m. pachydermatis, recovered from the skin of healthy dogs and dogs with dermatitis in brazil, were characterized on the basis of partial sequencing of the large subunit (lsu), first internal transcribed spacer (its-1) and chitin synthase 2 gene (chs-2). the determination of phospholipase production was also included in ... | 2010 | 20105099 |
| gentamycin inhibits the growth of malassezia pachydermatis in culture. | malassezia pachydermatis is a yeast of importance in both veterinary and human medicine. | 2010 | 20149702 |
| prevalence of malassezia species in pityriasis versicolor lesions in northeast argentina. | malassezia species normally colonize the skin but they can change their saprophytic state and invade the stratum corneum as pathogens. | 2010 | 20346299 |
| superficial veterinary mycoses. | dermatophytes are significant pathogens in animal health due to their zoonotic potential, the economic consequences of infection in farm animal and fur production systems, and the distressing lesions they cause in small domestic pets. malassezia spp are normal commensal and occasional pathogens of the skin of many veterinary species. malassezia pachydermatis is a very common cause of otitis and pruritic dermatitis in dogs but is of less importance in other veterinary species. dermatophytosis, an ... | 2010 | 20347667 |
| genotyping of malassezia pachydermatis isolates from canine healthy skin and atopic dermatitis by internal spacer 1 (igs1) region analysis. | isolates of malassezia pachydermatis from healthy dog skin and from dogs with atopic dermatitis were molecularly characterized using internal spacer 1 (igs1) region analyses, and their phospholipase a2 activity and ph growth profiles were then characterized in vitro. the percentage of isolates from healthy dogs that had the following igs1 subtypes (isotype, %) were as follows: 1a, 6%; 1b, 27%; 1c, 11%; 2a, 6%; 2b, 6%; 3a, 11%; 3c, 3%; and 3d, 24%. in contrast, 9% of isolates from dogs with atopi ... | 2011 | 21401740 |
| extracellular phospholipase activity of malassezia spp. isolated from individuals with and without dermatological disease. | background: the malassezia genus includes mainly lipophilic yeasts belonging to the cutaneous microbiota of man and other mammals. some malassezia species have been associated with various dermatological diseases. the factors permitting the transformation of yeasts of the malassezia genus from a commensal organism to a pathogenic agent are still little known but the production of various enzymes such as lipase, phospholipase and lipoxygenase could contribute to the pathogenic activity of these y ... | 2011 | 21645629 |
| in vitro susceptibility of fluconazole-susceptible and -resistant isolates of malassezia pachydermatis against azoles. | the first aim of this study was to evaluate the in vitro efficacies of fluconazole, ketoconazole, itraconazole and voriconazole on m. pachydermatis growth inhibition. this study also evaluated m. pachydermatis azole cross-resistance, comparing wild clinical isolates and the same isolates with in vitro-induced fluconazole resistance. | 2011 | 21658868 |
| comparative in vitro efficacy of antimicrobial shampoos: a pilot study. | this study compared the antimicrobial efficacy of shampoos against meticillin-sensitive staphylococcus pseudintermedius (mssp), meticillin-resistant s. pseudintermedius (mrsp), antibiotic-sensitive pseudomonas aeruginosa (pa), multidrug-resistant p. aeruginosa (mdr-pa) and malassezia pachydermatis. three isolates were incubated for 10, 30 and 60 min with each shampoo diluted in phosphate-buffered saline. aliquots were then incubated for 16-18 h on sheep blood agar (bacteria) or for 3 days on sab ... | 2011 | 21777309 |
| pyrosequencing of a hypervariable region in the internal transcribed spacer 2 to identify clinical yeast isolates. | the incidence of invasive fungal infection has increased significantly. a majority of the infections is caused by yeast. clinically important yeast show species-specific differences in susceptibility to antifungal agents therefore rapid and accurate identification of the pathogen is essential. we aimed to validate pyrosequencing of 40 nucleotides in the internal transcribed spacer 2 (its2) for species identification of yeast. amplification of its2 and pyrosequencing of targeted region were perfo ... | 2011 | 21736632 |