Publications

a multiplex pcr-based method to identify strongylid parasite larvae recovered from ovine faecal cultures and/or pasture samples.	a multiplex pcr-based method was developed to overcome the limitations of microscopic examination as a means of identifying individual infective larvae from the wide range of strongylid parasite species commonly encountered in sheep in mixed sheep-cattle grazing situations in new zealand. the strategy employed targets unique species-specific sequence markers in the second internal transcribed spacer (its-2) region of ribosomal dna of the nematodes and utilises individual larval lysates as reacti ...	2014	24373513
genetic differences between chabertia ovina and c. erschowi revealed by sequence analysis of four mitochondrial genes.	this study examined sequence differences in mitochondrial cytochrome c oxidase subunit 1 (cox1), large subunit ribosomal rna (rrnl) and nadh dehydrogenase subunits 1 and 4 (nad1 and nad4) between chabertia ovina and c. erschowi from yaks in qinghai and goats in shaanxi provinces, china. a part of the cox1 (pcox1), rrnl (prrnl), nad1 and nad4 genes (pnad1 and pnad4) were amplified separately from individual nematodes by pcr and sequenced. the length of the sequences of pcox1, prrnl, pnad1 and pna ...	2015	24328823
real-time pcr/dna melting curve-based assay to identify individual strongylid larvae recovered from ovine faecal cultures.	a closed-tube real-time pcr (rt pcr) method was developed to identify individual strongylid nematode larvae recovered from ovine faecal cultures. the method builds on an earlier conventional pcr assay established by our group and similarly targets species-specific sequence motifs in the its-2 region of ribosomal dna. the new procedure combines rt pcr with dna melting curve analyses to identify species-specific amplicons, thus avoiding the need to undertake gel electrophoresis. as with the earlie ...	2015	26526096
establishment rate of sheep gastrointestinal nematodes in farmed red deer (cervus elaphus).	to investigate the establishment of sheep gastrointestinal nematodes (gin) in red deer, five red deer and five sheep aged 5-6 months were challenged with a mixed burden of sheep gin at a rate of 327l3/kg bodyweight. the lsmean (se) establishment rates (%) for haemonchus contortus, teladorsagia circumcincta, cooperia curticei, trichostrongylus spp. and oesophagostomum+chabertia spp. were 18.6 (0.03), 35.5 (0.04), 30.7 (0.04), 74.9 (0.05), 19.9 (0.06), respectively in sheep and 10.5 (0.03), 1.0 (0 ...	2015	25657087
a field survey on the status of gastrointestinal helminth parasites in hangul (cervus elaphus hanglu) in dachigam national park of kashmir.	one year crossectional survey was carried out to determine and describe the prevalence and intensity of gastrointestinal parasite infections in hangul (cervus elaphus hanglu) in dachigam national park of kashmir through faecal examinations. out of 153 faecal samples examined, 82 (53.59 %) were found infected with git helminthes. in present study seven helminth species were found, including five nematode [haemonchus contortus (55.39 %), trichuris ovis (39.75 %), dictyocaulus viviparus (28.4.00 %) ...	2016	27605778
use of fluorescent lectin binding to distinguish eggs of gastrointestinal nematode parasites of sheep.	the binding of a panel of 19 lectins to carbohydrates on the eggs of economically important nematode parasites of sheep has been assessed as the basis of a rapid test to distinguish parasite eggs, at least at the genus level. a total of six lectins can be used to identify eggs of six nematode parasites: peanut agglutinin (pna) for haemonchus contortus; lens culinaris agglutinin (lca) for teladorsagia sp; aleuria aurantia agglutinin (aal) for trichostrongylus sp; psophocarpus tetragonolobus‑ii (p ...	2016	26827865
eprinomectin pour-on (eprinex® pour-on, merial): efficacy against gastrointestinal and pulmonary nematodes and pharmacokinetics in sheep.	the anthelmintic efficacy of the 0.5% w/v topical formulation of eprinomectin (epn), eprinex® pour-on (merial) when administered at 1 mg/kg body weight was evaluated in sheep in two dose confirmation laboratory studies and one multicenter field study. in addition, the pharmacokinetics of epn when administered at that dosage to adult sheep was determined.	2017	28558828