Publications

ensifer meliloti bv. lancerottense establishes nitrogen-fixing symbiosis with lotus endemic to the canary islands and shows distinctive symbiotic genotypes and host range.	eleven strains were isolated from root nodules of lotus endemic to the canary islands and they belonged to the genus ensifer, a genus never previously described as a symbiont of lotus. according to their 16s rrna and atpd gene sequences, two isolates represented minority genotypes that could belong to previously undescribed ensifer species, but most of the isolates were classified within the species ensifer meliloti. these isolates nodulated lotus lancerottensis, lotus corniculatus and lotus jap ...	2009	19477097
agrobacterium rhizogenes-mediated transformation of superroot-derived lotus corniculatus plants: a valuable tool for functional genomics.	transgenic approaches provide a powerful tool for gene function investigations in plants. however, some legumes are still recalcitrant to current transformation technologies, limiting the extent to which functional genomic studies can be performed on. superroot of lotus corniculatus is a continuous root cloning system allowing direct somatic embryogenesis and mass regeneration of plants. recently, a technique to obtain transgenic l. corniculatus plants from superroot-derived leaves through a. tu ...	2009	19555486
the incomplete substitution of lipopolysaccharide with o-chain prevents the establishment of effective symbiosis between mesorhizobium loti nzp2213.1 and lotus corniculatus.	mesorhizobium loti nzp2213.1 mutant obtained after random tn5 mutagenesis of m. loti nzp2213 was inefficient in nitrogen fixation on lotus corniculatus. the transposon insertion was located within an orf with a sequence similarity to a putative glycosyl transferase from caulobacter crescentus. the results of sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that the mutant produced lps of the same o-chain length but only half of the entire smooth lps, compared to that of the pa ...	2009	17321732
performance and dietary preferences of white-tailed deer grazing chicory, birdsfoot trefoil or alfalfa in north central alberta.	little information exists on the performance of deer on alternative forage species in northern temperate environments during summer and fall, the period of inherent maximum growth in deer. in performance and choice experiments, we compared live weight gain (g/kg(0.75)/day), absolute [kg/ha dry matter (dm)] and relative (% dm) herbage utilization, relative preference index (rpi) as well as plant community visitation of white-tailed deer grazing alfalfa (medicago sativa), birdsfoot trefoil (lotus ...	2009	19138349
short-term nutritional treatments grazing legumes or feeding concentrates increase prolificacy in corriedale ewes.	we tested whether short periods of increased nutrition will improve ovulation rate and prolificacy, irrespective of the method used to synchronise the cycles of the ewes. in experiment 1, we used 138 corriedale ewes to evaluate two factors: synchronization treatment (sponges versus a single injection of prostaglandin) and type of pasture (native versus improved with lotus corniculatus). ewes were mated at the end of the grazing period and prolificacy was evaluated at lambing. grazing lotus corni ...	2009	18639397
454 pyrosequencing based transcriptome analysis of zygaena filipendulae with focus on genes involved in biosynthesis of cyanogenic glucosides.	an essential driving component in the co-evolution of plants and insects is the ability to produce and handle bioactive compounds. plants produce bioactive natural products for defense, but some insects detoxify and/or sequester the compounds, opening up for new niches with fewer competitors. to study the molecular mechanism behind the co-adaption in plant-insect interactions, we have investigated the interactions between lotus corniculatus and zygaena filipendulae. they both contain cyanogenic ...	2009	19954531
technical note: effects of forage protein-binding polyphenols on chemistry of dairy excreta.	forage chemistry can affect intake, digestion, milk production, and manure excretion. although information is available on the effects of forage protein-binding polyphenols on small ruminant production and manure excretion, little information is available for dairy cattle. the objective of this study was to compare fecal and urinary n excretion of diets formulated with alfalfa (medicago sativa l.) silage versus condensed tannin-containing birdsfoot trefoil (lotus corniculatus) or o-quinone-conta ...	2009	19307659
two bee-pollinated plant species show higher seed production when grown in gardens compared to arable farmland.	insect pollinator abundance, in particular that of bees, has been shown to be high where there is a super-abundance of floral resources; for example in association with mass-flowering crops and also in gardens where flowering plants are often densely planted. since land management affects pollinator numbers, it is also likely to affect the resultant pollination of plants growing in these habitats. we hypothesised that the seed or fruit set of two plant species, typically pollinated by bumblebees ...	2010	20668704
characterization of strains unlike mesorhizobium loti that nodulate lotus spp. in saline soils of granada, spain.	lotus species are forage legumes with potential as pastures in low-fertility and environmentally constrained soils, owing to their high persistence and yield under those conditions. the aim of this work was the characterization of phenetic and genetic diversity of salt-tolerant bacteria able to establish efficient symbiosis with lotus spp. a total of 180 isolates able to nodulate lotus corniculatus and lotus tenuis from two locations in granada, spain, were characterized. molecular identificatio ...	2010	20435777
lotus tenuis tolerates combined salinity and waterlogging: maintaining o2 transport to roots and expression of an nhx1-like gene contribute to regulation of na+ transport.	salinity and waterlogging interact to reduce growth for most crop and pasture species. the combination of these stresses often cause a large increase in the rate of na(+) and cl(-) transport to shoots; however, the mechanisms responsible for this are largely unknown. to identify mechanisms contributing to the adverse interaction between salinity and waterlogging, we compared two lotus species with contrasting tolerances when grown under saline (200 mm nacl) and o(2)-deficient (stagnant) treatmen ...	2010	20444189
the effects of condensed tannins extracted from different plant species on egg hatching and larval development of teladorsagia circumcincta (nematoda: trichostrongylidae).	the effects of condensed tannins (cts) extracted from five species of plants on egg hatching and larval development of teladorsagia circumcincta (stadelmann, 1894) (syn. ostertagia circumcincta) were evaluated using in vitro bioassays. the extracts of cts were obtained from lotus pedunculatus (lp), lotus corniculatus (lc), dorycnium pentaphyllum (dp), dorycnium rectum (dr) and rumex obtusifolius (ro). the results of egg hatching assay showed that about 53%, 68%, 51%, 60% and 46% of the eggs hatc ...	2010	20450000
hydrocarbon degradation and plant colonization by selected bacterial strains isolated from italian ryegrass and birdsfoot trefoil.	to assess the degradation potential and plant colonization capacity of four alkane-degrading strains (itsi10, itri15, itrh76 and btrh79) in combination with birdsfoot trefoil and italian ryegrass and to evaluate the diversity of indigenous alkane-degrading soil bacteria in the rhizo- and endosphere.	2010	20522148
metabolomics unravel contrasting effects of biodiversity on the performance of individual plant species.	in spite of evidence for positive diversity-productivity relationships increasing plant diversity has highly variable effects on the performance of individual plant species, but the mechanisms behind these differential responses are far from being understood. to gain deeper insights into the physiological responses of individual plant species to increasing plant diversity we performed systematic untargeted metabolite profiling on a number of herbs derived from a grassland biodiversity experiment ...	2010	20830202
fox-superroots of lotus corniculatus, overexpressing arabidopsis full-length cdna, show stable variations in morphological traits.	using the full-length cdna overexpressor (fox) gene-hunting system, we have generated 130 arabidopsis fox-superroot lines in bird's-foot trefoil (lotus corniculatus) for the systematic functional analysis of genes expressed in roots and for the selection of induced mutants with interesting root growth characteristics. we used the arabidopsis-fox agrobacterium library (constructed by ligating pbig2113sf) for the agrobacterium-mediated transformation of superroots (sr) and the subsequent selection ...	2010	21106274
lotus corniculatus regulates the inflammation induced by bradykinin in a murine model of pleurisy.	this study evaluated the anti-inflammatory efficacy of the crude extract (ce), the fractions derived from hexane (hex), ethyl acetate (acoet), n-butanol (buoh), and aqueous (aq) and isolated compounds (oleanolic acid or kaempferitrin) obtained from the aerial parts of lotus corniculatus var. sa~o gabriel in mice with bradykinin-induced pleurisy. swiss mice were used for the in vivo experiments. inflammatory parameters [leukocytes; exudate concentrations; myeloperoxidase and adenosine-deaminase a ...	2011	21355560
genetic diversity of root nodule bacteria nodulating lotus corniculatus and anthyllis vulneraria in sweden.	very little is known about the genetic diversity and phylogeny of rhizobia nodulating lotus species in northern temperate regions. we have therefore studied the genetic diversity among a total of 61 root nodule bacteria isolated from lotus corniculatus and anthyllis vulneraria from different geographic sites and habitats in sweden by restriction fragment length polymorphism (rflp) of the internal transcribed spacer between their 16s rrna and 23s rrna (igs) region. a high diversity consisting of ...	2011	21497473
isolation and characterization of two strains of fusarium oxysporum causing potato dry rot in solanum tuberosum in colombia.	background: fusarium oxysporum has worldwide distribution and causes severe vascular wilt or root rot in many plants. strains are classified into formae speciales based on their high degree of host specificity, of which multilocus sequence typing provides a fairly good estimate. aims: the main aim of this study was to identify the causal agent of an infected potato tuber in colombia. methodology and results: two f. oxysporum isolates were recovered from a potato tuber showing symptoms of dry rot ...	2011	21635960
phylogenetic relationship of lotus uliginosus symbionts with bradyrhizobia nodulating genistoid legumes.	lotus species are legumes with potential for pastures in soils with low-fertility and environmental constraints. the aim of this work was to characterize bacteria that establish efficient nitrogen-fixing symbiosis with the forage species lotus uliginosus. a total of 39 isolates were obtained from nodules of l. uliginosus naturally growing in two different locations of portugal. molecular identification of the isolates plus the commercial inoculant strain nzp2039 was performed by rep-pcr, 16s rrn ...	2011	22092879
condensed tannins act against cattle nematodes.	the use of natural plant anthelmintics was suggested as a possible alternative control of gastrointestinal nematodes (gin) in ruminants. direct anthelmintic effects of tannin-containing plants have already been shown in sheep and goat gin. these anthelmintic properties are mainly associated with condensed tannins. in the present study, we evaluated possible in vitro effects of three tannin-containing plants against bovine gin. effects of onobrychis viciifolia, lotus pedunculatus and lotus cornic ...	2011	21726942
promex - a mass spectral reference database for plant proteomics.	the promex database is one of the main collection of annotated tryptic peptides in plant proteomics. the main objective of the promex database is to provide experimental ms/ms-based information for cell type-specific or sub-cellular proteomes in arabidopsis thaliana, medicago truncatula, chlamydomonas reinhardtii, lotus japonicus, lotus corniculatus, phaseolus vulgaris, lycopersicon esculentum, solanum tuberosum, nicotiana tabacum, glycine max, zea mays, bradyrhizobium japonicum, and sinorhizobi ...	2012	22685450
polymorphic infection and organogenesis patterns induced by a rhizobium leguminosarum isolate from lotus root nodules are determined by the host genotype.	to sample the natural variation in genes controlling compatibility in the legume-rhizobium symbiosis, we isolated rhizobia from nodules of endemic lotus species from 21 sites across europe. the majority of isolates were identified as mesorhizobium- or bradyrhizobium-related and formed nitrogen-fixing root nodules on lotus corniculatus and l. pendunculatus, respectively, thus confirming previously defined cross-inoculation groups. rhizobium leguminosarum (rl) strain norway, isolated from l. corni ...	2012	22950721
improvement of drought and salt tolerance in arabidopsis and lotus corniculatus by overexpression of a novel dreb transcription factor from populus euphratica.	a novel dreb (dehydration-responsive element binding) gene, designated pedreb2a, was isolated from the desert-grown tree, populus euphratica oliv. pedreb2a is classified into the a-5 group of dreb subfamily based on multiple sequence alignment and phylogenetic characterization. using semi-quantitative rt-pcr, we found that the pedreb2a was greatly induced by drought, nacl, low temperature, 1-naphthaleneacetic acid (naa), 6-benzyl aminopurine (6-ba) and gibberellic acid (ga3) treatments in p. eup ...	2012	22771912
increased plant carbon translocation linked to overyielding in grassland species mixtures.	plant species richness and productivity often show a positive relationship, but the underlying mechanisms are not fully understood, especially at the plant species level. we examined how growing plants in species mixture influences intraspecific rates of short-term carbon (c-) translocation, and determined whether such short-term responses are reflected in biomass yields. we grew monocultures and mixtures of six common c3 grassland plant species in outdoor mesocosms, applied a (13)c-co(2) pulse ...	2012	23049893
two years research on efficiency of two intercrops, birdsfoot trefoil and summer savory, to reduce damage caused by onion thrips(thrips tabaci lindeman, thysanoptera, thripidae) on leek.	in 2009 and 2011, a field experiment was carried out at the laboratory field at the biotechnical faculty in ljubljana, slovenia, with the aim to investigate suitability of two intercrops, birdsfoot trefoil (lotus corniculatus l) and summer savory (satureja hortensis l.), for reducing damage caused by onion thrips (thrips tabaci lindeman) on leek (allium porrum l.). four leek cultivars--'columbus', 'forrest', 'lancelot' and 'lincoln'--were used in the research (bejo zaden b.v., netherlands). in b ...	2012	23885428
an integrated approach to demonstrating the anr pathway of proanthocyanidin biosynthesis in plants.	proanthocyanidins (pas) are oligomers or polymers of plant flavan-3-ols and are important to plant adaptation in extreme environmental conditions. the characterization of anthocyanidin reductase (anr) and leucoanthocyanidin reductase (lar) has demonstrated the different biogenesis of four stereo-configurations of flavan-3-ols. it is important to understand whether anr and the anr pathway widely occur in the plant kingdom. here, we report an integrated approach to demonstrate the anr pathway in p ...	2012	22678031
herbaceous vegetation productivity, persistence, and metals uptake on a biosolids-amended mine soil.	the selection of plant species is critical for the successful establishment and long-term maintenance of vegetation on reclaimed surface mined soils. a study was conducted to assess the capability of 16 forage grass and legume species in monocultures and mixes to establish and thrive on a reclaimed appalachian surface mine amended with biosolids. the 0.15-ha coarse-textured, rocky, non-acid forming mined site was prepared for planting by grading to a 2% slope and amending sandstone overburden ma ...	2012	16151233
fatty acids, α-tocopherol, β-carotene, and lutein contents in forage legumes, forbs, and a grass-clover mixture.	fresh forages are an important natural source of vitamins and fatty acids in ruminant diets, and their concentrations in forage species are important for the quality of animal-derived foods such as dairy and meat products. the aims of this study were to obtain novel information on vitamins and fatty acids (fa) in a variety of forage legumes and non-legume forb species compared to a grass-clover mixture and to explore implications for animal-derived products. seven dicotyledons [four forbs (salad ...	2013	24262011
suitability of live yeast addition to alleviate the adverse effects due to the restriction of the time of access to feed in sheep fed only pasture.	the effect of yeast addition on intake and digestive utilization of pasture was studied in ovines under restricted time of access to forage. eighteen wethers housed in metabolic cages and fed fresh forage (predominantly lotus corniculatus) were randomly assigned to three treatments: forage available all day (ad); forage available only 6 h/day (r) and forage available only 6 h/day plus live saccharomyces cerevisiae yeast (ry). feed intake and digestibility, feeding behaviour, kinetics of passage, ...	2013	23020124
antioxidant and photosystem ii responses contribute to explain the drought-heat contrasting tolerance of two forage legumes.	identification of metabolic targets of environmental stress factors is critical to improve the stress tolerance of plants. studying the biochemical and physiological responses of plants with different capacities to deal with stress is a valid approach to reach this objective. lotus corniculatus (lotus) and trifolium pratense (clover) are legumes with contrasting summer stress tolerances. in stress conditions, which are defined as drought, heat or a combination of both, we found that differential ...	2013	23792824
in situ protein degradation of alfalfa and birdsfoot trefoil hays and silages as influenced by condensed tannin concentration.	dairy cattle often make poor use of protein when offered diets comprising high proportions of alfalfa (medicago sativa l.) hay or silage because nonprotein n formed during forage conservation and ruminal fermentation exceeds requirements for rumen microbial protein synthesis; however, condensed tannins (ct) may reduce proteolysis in the silo and in the rumen, thereby potentially improving the efficiency of crude protein (cp) use in ruminant diets. two harvests, yielding 12 hays and 12 silages ma ...	2013	23477815
conditional requirement for exopolysaccharide in the mesorhizobium-lotus symbiosis.	rhizobial surface polysaccharides are required for nodule formation on the roots of at least some legumes but the mechanisms by which they act are yet to be determined. as a first step to investigate the function of exopolysaccharide (eps) in the formation of determinate nodules, we isolated mesorhizobium loti mutants affected in various steps of eps biosynthesis and characterized their symbiotic phenotypes on two lotus spp. the wild-type m. loti r7a produced both high molecular weight eps and l ...	2013	23134480
phyllobacterium loti sp. nov. isolated from nodules of lotus corniculatus.	strain s658(t) was isolated from a lotus corniculatus nodule in a soil sample obtained in uruguay. phylogenetic analysis of the 16s rrna gene and atpd gene showed that this strain clustered within the genus phyllobacterium. the closest related species was, in both cases, phyllobacterium trifolii petp02(t) with 99.8 % sequence similarity in the 16s rrna gene and 96.1 % in the atpd gene. the 16s rrna gene contains an insert at the beginning of the sequence that has no similarities with other inser ...	2014	24271211
genome sequence of the lotus corniculatus microsymbiont mesorhizobium loti strain r88b.	mesorhizobium loti strain r88b was isolated in 1993 in the rocklands range in otago, new zealand from a lotus corniculatus root nodule. r88b is an aerobic, gram-negative, non-spore-forming rod. this report reveals the genome of m. loti strain r88b contains a single scaffold of size 7,195,110 bp which encodes 6,950 protein-coding genes and 66 rna-only encoding genes. this genome does not harbor any plasmids but contains the integrative and conjugative element icemlsym(r7a), also known as the r7a ...	2014	25780496
genome sequence of the lotus spp. microsymbiont mesorhizobium loti strain r7a.	mesorhizobium loti strain r7a was isolated in 1993 in lammermoor, otago, new zealand from a lotus corniculatus root nodule and is a reisolate of the inoculant strain icmp3153 (nzp2238) used at the site. r7a is an aerobic, gram-negative, non-spore-forming rod. the symbiotic genes in the strain are carried on a 502-kb integrative and conjugative element known as the symbiosis island or icemlsym(r7a). m. loti is the microsymbiont of the model legume lotus japonicus and strain r7a has been used exte ...	2014	25780499
the trehalose utilization gene thua ortholog in mesorhizobium loti does not influence competitiveness for nodulation on lotus spp.	competitiveness for nodulation is a desirable trait in rhizobia strains used as inoculant. in sinorhizobium meliloti 1021 mutation in either of the trehalose utilization genes thua or thub influences its competitiveness for root colonization and nodule occupancy depending on the interacting host. we have therefore investigated whether mutation in the thua ortholog in mesorhizobium loti maff303099 also leads to a similar competitive phenotype on its hosts. the results show that m. loti thua mutan ...	2014	24142427
does the invasive lupinus polyphyllus increase pollinator visitation to a native herb through effects on pollinator population sizes?	invasive plants may compete with native species for abiotic factors as light, space and nutrients, and have also been shown to affect native pollination interactions. studies have mainly focused on how invasive plants affect pollinator behaviour, i.e. attraction of pollinators to or away from native flowers. however, when an invasive plant provides resources utilized by native pollinators this could increase pollinator population sizes and thereby pollination success in natives. effects mediated ...	2014	24061551
determination of nutrient contents and in vitro gas production values of some legume forages grown in the harran plain saline soils.	the aim of this study was to determine the nutritive value of some legume species in salt-affected soils of south-east anatolian region using chemical composition and in vitro gas production kinetics. in this study, lotus corniculatus, trifolium alexandrinum, medicago sativa were sown and tested in four different locations. a 3 by 4 factorial design with 3 legume species and 4 salt levels (non salty electrical conductivity (ec)<4 ds/m; low salt: 4 ds/m>ec<8 ds/m, medium saline: 8 ds/m>ec<16 ds/m ...	2014	25050020
genome sequence of ensifer arboris strain lmg 14919(t); a microsymbiont of the legume prosopis chilensis growing in kosti, sudan.	ensifer arboris lmg 14919(t) is an aerobic, motile, gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of several species of legume trees. lmg 14919(t) was isolated in 1987 from a nodule recovered from the roots of the tree prosopis chilensis growing in kosti, sudan. lmg 14919(t) is highly effective at fixing nitrogen with p. chilensis (chilean mesquite) and acacia senegal (gum arabic tree or gum acacia). lmg 14919(t) does not nodulate the tree ...	2014	25197433
in vitro screening of forty medicinal plant extracts from the united states northern great plains for anthelmintic activity against haemonchus contortus.	an egg hatch assay (eha) and a larval migration assay (lma) involving haemonchus contortus was used to evaluate the anthelmintic activity of methanol extracts from 40 plants that are native or naturalized within the u.s.a. northern great plains. only one of these 40 plants (i.e. lotus corniculatus) had been previously evaluated for activity against any gastrointestinal nematode. the various extracts were initially screened at 50mg/ml diluted either in 0.5% dimethyl sulphoxide (dmso) or 3-(n-morp ...	2014	24548703
interrelations between herbage yield, α-tocopherol, β-carotene, lutein, protein, and fiber in non-leguminous forbs, forage legumes, and a grass-clover mixture as affected by harvest date.	pastures with diverse botanical composition may enhance animal-derived product quality. a recent study demonstrated high vitamin concentrations and yields in some forb species. the objectives of the present study were to investigate interrelations between herbage yields, vitamin concentrations, protein and fiber contents and analyze the effect of harvest date. we hypothesized that interrelations would be similar across investigated forage species. four nonleguminous forbs: salad burnet (sanguiso ...	2015	25573460
carcass parameters and meat quality in meat-goat kids finished on chicory, birdsfoot trefoil, or red clover pastures.	this study was conducted during the 2009 and 2010 grazing seasons to assess carcass parameters and chevon (goat meat) quality when meat-goat kids (n=72) were finished on pastures of red clover (trifolium pratense l.; rcl), birdsfoot trefoil (lotus corniculatus l.; bft), or chicory (cichorium intybus l.; chic). final live weight (p<0.05) and carcass weight (p=0.10) were greater when goats were finished on rcl compared to chic with bft being intermediate. ribeye area, backfat thickness, body wall ...	2015	25817803
soil-to-plant transfer of native selenium for wild vegetation cover at selected locations of the czech republic.	total selenium (se) contents were determined in aboveground biomass of wild plant species growing in two uncultivated meadows at two different locations. the soils in these locations had pseudototal (aqua regia soluble) se in concentration ranges of between 0.2 and 0.3 mg kg(-1) at the first location, and between 0.7 and 1.4 mg kg(-1) at the second location. the plant species represented 29 plant families where the most numerous ones were poaceae, rosaceae, fabaceae , and asteraceae. the seleniu ...	2015	25975239
revision of the taxonomic status of type strains of mesorhizobium loti and reclassification of strain usda 3471t as the type strain of mesorhizobiumerdmanii sp. nov. and atcc 33669t as the type strain of mesorhizobiumjarvisii sp. nov.	the species mesorhizobim loti was isolated from nodules of lotus corniculatus and its type strain deposited in several collections. some of these type strains, such as those deposited in the usda and atcc collections before 1990, are not coincident with the original strain, nzp 2213t, deposited in the nzp culture collection. the analysis of the 16s rrna gene showed that strains usda 3471t and atcc 33669t formed independent branches from that occupied by mesorhizobium loti nzp 2213t and related t ...	2015	25736411
growth and survival of mesorhizobium loti inside acanthamoeba enhanced its ability to develop more nodules on lotus corniculatus.	the importance of protozoa as environmental reservoirs of pathogens is well recognized, while their impact on survival and symbiotic properties of rhizobia has not been explored. the possible survival of free-living rhizobia inside amoebae could influence bacterial abundance in the rhizosphere of legume plants and the nodulation competitiveness of microsymbionts. two well-characterized strains of mesorhizobium: mesorhizobium loti nzp2213 and mesorhizobium huakuii symbiovar loti maff303099 were a ...	2015	25779926
effect of exchanging onobrychis viciifolia and lotus corniculatus for medicago sativa on ruminal fermentation and nitrogen turnover in dairy cows.	the objective of the study was to determine the effect of feeding sainfoin (sf; onobrychis viciifolia) and birdsfoot trefoil (bt; lotus corniculatus), 2 temperate climate forage legumes that contain condensed tannins (ct), on ruminal fermentation and n turnover in dairy cows. six ruminally cannulated multiparous dairy cows (milk yield=40kg/d; 36 d in milk) were used in a replicated 3×3 latin square design. all animals were fed basal diets containing 20% pelleted sf (223g of ct/kg of dry matter), ...	2016	26995129
chronic exposure to a neonicotinoid pesticide alters the interactions between bumblebees and wild plants.	insect pollinators are essential for both the production of a large proportion of world crops and the health of natural ecosystems. as important pollinators, bumblebees must learn to forage on flowers to feed both themselves and provision their colonies.increased use of pesticides has caused concern over sublethal effects on bees, such as impacts on reproduction or learning ability. however, little is known about how sublethal exposure to field-realistic levels of pesticide might affect the abil ...	2016	27512241
plants increase arsenic in solution but decrease the non-specifically bound fraction in the rhizosphere of an alkaline, naturally rich soil.	we aimed at determining the major physical-chemical processes that drive arsenic (as) dynamic in the rhizosphere of four species (holcus lanatus, dittrichia viscosa, lotus corniculatus, plantago lanceolata) tested for phytostabilization. experiments were performed with an alkaline soil naturally rich in as. composition of the soil solution of planted and unplanted pots was monitored every 15 days for 90 days, with a focus on the evolution of as concentrations in solution and in the non-specifica ...	2016	26707185
therapeutic potential of temperate forage legumes: a review.	the discovery of bioactive molecules from botanical sources is an expanding field, preferentially oriented to plants having a tradition of use in medicine and providing high yields and availability. temperate forage legumes are fabaceae species that include worldwide-important crops. these plants possess therapeutic virtues that have not only been used in veterinary and folk medicine, but have also attracted the interest of official medicine. we have examined here medicago sativa (alfalfa), trif ...	2016	26507574
mesorhizobium olivaresii sp. nov. isolated from lotus corniculatus nodules.	in this study four mesorhizobium strains isolated from lotus corniculatus nodules in granada (spain) were characterized. their 16s rrna gene sequences were closely related to those of m. albiziae lmg 23507(t) and m. chacoense pr5(t) showing 99.4 and 99.2% similarity values, respectively. the analysis of concatenated rpob, reca, atpd and glnii genes showed they formed a cluster with internal similarities higher than 97%. the closest species also were m. albiziae lmg 23507(t) and m. chacoense pr5( ...	2016	27712916
compost and calcium surface treatment effects on subsoil chemistry in acidic minespoil columns.	surface incorporation of a liming agent in combination with compost or biosolids is a proven way to revegetate acidic minespoils, but little is known about the effect of the surface amendments on subsoil chemistry. we conducted a greenhouse column experiment to investigate how different surface amendments affected plant growth and subsoil chemistry in highly acidic minespoil material. columns were filled with shale minespoil material (ph approximately 2.5), amended with caco3, caso4 x 2h2o (gyps ...	2016	12809279
replacing alfalfa silage with tannin-containing birdsfoot trefoil silage in total mixed rations for lactating dairy cows.	two lactation trials were conducted comparing the feeding value of silages made from birdsfoot trefoil (bft, lotus corniculatus l.) that had been selected for low (bftl), medium (bftm), and high (bfth) levels of condensed tannins (ct) to an alfalfa silage (as) when fed as the principal forage in total mixed rations. diets also included corn silage, high-moisture shelled corn, soybean meal, soy hulls, and supplemental fat. in trial 1, 32 lactating holstein cows were blocked by days in milk, assig ...	2017	28259401