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clastogenicity of red pepper (capsicum frutescens l.) extracts.extracts from the fruits of capsicum frutescens l. were tested for their clastogenicity using the mouse-bone-marrow micronucleus (mouse-mn) assay. results of the mouse-mn, an in vivo method, indicated that the isolate cf-1 is clastogenic at the maximum tolerated dose of 1.22 mg/kg mouse. statistical analysis using the wilcoxon two-sample test showed that the null hypothesis, mu tetracycline = mucf-1, is acceptable at 0.05 and 0.01 degrees of significance. hence, the clastogenicity of cf-1 is sta ...19947510827
identification of a plastid protein involved in vesicle fusion and/or membrane protein translocation.structural evidence has accumulated suggesting that fusion and/or translocation factors are involved in plastid membrane biogenesis. to test this hypothesis, we have developed an in vitro system in which the extent of fusion and/or translocation is monitored by the conversion of the xanthophyll epoxide (antheraxanthin) into the red ketocarotenoid (capsanthin). only chromoplast membrane vesicles from red pepper fruits (capsicum annuum) contain the required enzyme. vesicles prepared from the mutan ...19957777561
effects of red-pepper diet on the energy metabolism in men.we investigated the effects of dietary red pepper on the energy metabolism in male subjects. in the first experiment, after having a standardized dinner on the previous evening, the subjects consumed a breakfast (650 kcal) either with or without 10 g of red pepper. for 150 min after the meal, they took a rest and their expired gas was collected. during the initial 30 min after the meal, the energy expenditure tended to be higher in the red-pepper diet period than in the control diet period. for ...19958926537
[allergy associated with pepper and latex: new cross reaction?].the aim of this study was to point out the frequency of associated sensitization to rubber latex and to sweet pepper. the study included three populations of patients: patients allergic to rubber latex, patients sensitized to rubber latex and patients without any clinical symptoms for rubber latex and sweet pepper (negative controls). we pointed out a high frequency of patients sensitized to sweet pepper among the patients allergic or sensitized to rubber latex which lead to the hypothesis of a ...19958851036
natural occurrence of mycotoxins in different spices in egypt.a total of 120 different samples belonging to 24 kinds of species collected from different places at assiut governorate (egypt) were examined for the natural occurrence of mycotoxins. tlc analysis of spice extracts revealed the presence of aflatoxins (8-35 micrograms/kg) in 16 samples of anise, black pepper, caraway, black cumin, fennel, peppermint, coriander and marjoram, sterigmatocystin (10-23 micrograms/kg) in ten samples of red pepper, caraway, cumin and marjoram and citrinin (8-12 microgra ...19958919936
capsaicin, a double-edged sword: toxicity, metabolism, and chemopreventive potential.capsaicin (8-methyl-n-vanillyl-6-nonenamide) is a primary pungent and irritating principle present in chilies and red peppers which are widely used as spices. because of its selective effects on the functions of a defined subpopulation of sensory neurons, capsaicin is currently used as a versatile tool for the study of pain mechanisms and also for pharmacotherapy to treat several pain disorders. considering the frequent consumption of capsaicin as a food additive and its current medicinal use, c ...19957746093
red pepper effect. 19968995873
evidence for two types of phosphate translocators in sweet-pepper (capsicum annum l.) fruit chromoplasts.we have investigated whether there is evidence for the presence of different types of phosphate translocators in envelopes purified from pepper-fruit chromoplasts. a method was developed that allowed the purification of envelope membranes from isolated pepper-fruit chromoplasts. proteoliposomes containing envelope-membrane proteins are able to import inorganic phosphate (p1) or glucose 6-phosphate (glc6p). in both cases, the rate of import is strongly dependent upon preloading of proteoliposomes ...19968947460
screening of aflatoxins in shiro and ground red pepper in addis ababa.aflatoxin contamination of shiro and ground red pepper samples collected from government owned good stores, retail shops and open markets in. addis ababa was investigated. from sixty samples each of ground red pepper and shiro, 8 (13.33%) and 5 (8.33%) were positive for aflatoxins, respectively. only aflatoxin b1 was detected in both types of foodstuff. there was no significant difference between the proportion of aflatoxin contained in both ground red pepper and shiro samples. aflatoxin levels ...19969164040
[daily intake of carotenoids (carotenes and xanthophylls) from total diet and the carotenoid content of selected vegetables and fuit].the recommended daily intake is currently either 2 mg of beta-carotene (recommended by dge, germany, in addition to 1.0 (0.8) mg retinol-equivalents for vitamin a requirement) or 5-6 mg of beta-carotene (recommended by nci, usa). the present studies were carried out to investigate to what extent a balanced diet prepared using household or cafeteria methods contributes to achieve the desired intake. beta-carotene and other carotenoids in the total daily diet samples were determined by rp-hplc. in ...19968815648
red pepper-induced dermatitis in breast-fed infants.we report a transient, erythematous dermatitis that formed in 2 infants shortly after breast-feeding from their mothers who had ingested food flavored with red pepper, although the mothers did not display any signs of dermatitis.19968864625
major fruit and vegetable contributors to the main serum carotenoids in the spanish diet.to identify the main sources of serum carotenoids (lutein, zeaxanthin, lycopene, beta-cryptoxanthin, alpha-carotene and beta-carotene) among the fruits and vegetables in the spanish diet, to be used in the design of food questionnaires and to provide useful information for epidemiological and comparative studies.19968730612
capsaicin does not inhibit the intracellular calcium handling process in rat ventricular papillary muscle.1. we studied the effects of capsaicin, a pungent agent extracted from red pepper, on rested-state contraction (rsc) of isolated rat ventricular papillary muscles. 2. the rsc was induced by stimulation, after a rest interval of 5 sec to 10 min, after the twitch tension of the muscle preparation stimulated at the regular stimulus frequency of cycle lengths of 5, 1 or 0.2 sec attained the steady state. 3. drug effects were evaluated on the rsc in the presence of capsaicin 10(-5) m, caffeine 10(-2) ...19968742504
inhibition of contractile tension by capsaicin in isolated rat papillary muscle.1. we examined effects of capsaicin (10(-9) - 10(-5) m), a pungent agent extracted from red pepper, on the contractile tension of rat ventricular papillary muscles stimulated at various cycle lengths (0.2, 0.5, 1, 2 and 5 sec). 2. capsaicin produced a marked concentration-dependent decrease in the amplitude, the rate of rise (dp/dt) and the rate of relaxation (dr/dt) of the tension. 3. however, the half relaxation time and the time to peak tension of the tension were slightly affected by the age ...19968742509
influence of sundrying on the chemical composition, aflatoxin content and fungal counts of two pepper varieties--capsicum annum and capsicum frutescens.samples of sundried, matured red pepper, capsicum annum with a moisture content (mc) of 12.7-26.8 percent had on dry weight basis, vitamin c, 5.0-6.4 mg/100 g; crude protein, 0.8-1.2 percent; total soluble solids, 3.3-4.1 percent, and fungal counts of log 4.4-4.5/g. ordinary matured red c. annum had mc, 75.7-78.2 percent vitamin c, 36.1-38.5 mg/100 g; crude protein, 2.4-2.8 percent; total soluble solids, 9.3-9.9 percent and fungal count of log 3.32-3.39/g. sundried matured red c. frutescens had ...19968811723
capsaicin in diet does not affect glycogen contents in the liver and skeletal muscle of rats before and after exercise.effects of capsaicin, a pungent principle of hot red pepper, on glycogen contents in the liver and skeletal muscle at rest and during exercise were studied in rats. the contents of glycogen in the liver and soleus muscle, and the concentrations of serum glucose, lactate, free fatty acid and glycerol were examined. capsaicin was supplemented at 0.014% of the experimental diet. each group of rats was fed the capsaicin-diet ad libitum for 7 days, and then both groups of rats were fed isoenergetic d ...19968866261
capsaicin or feeding with red peppers during gestation changes the thermonociceptive response of rat offspring.capsaicin is responsible for the pungent sensation produced by red peppers on the body's mucous membranes. this substance is found naturally in the gender capsicum, widely used in the diet of different cultures in america, asia, and africa. in this paper we used the hot plate model (53 +/- 0.5 degrees c) to study the effect of acute thermonociceptive stimulus on escape response latency in the offspring of rats that were treated during gestation, either with an aqueous red pepper solution (capsic ...19968840903
transgenic sweet pepper plants fromagrobacterium mediated transformation.fertile transgenic sweet pepper (capsicum annuum var. grossum) plants were regenerated at relatively high rate from various explants that were cocultivated withagrobacterium tumefaciens strain gv3111-se harbouring a plasmid that contains the cucumber mosaic virus coat protein (cmv-cp) gene. the rate of plant regeneration was found to depend on the types of explants cultured and the media used. young leaves were most effective for bud induction and subsequent plant elongation while hypocotyls wer ...199624178658
capsaicin in hot chili pepper: carcinogen, co-carcinogen or anticarcinogen?capsaicin (trans-8-methyl-n-vanillyl-6-nonenamide) is a major pungent ingredient of the capsicum fruits such as hot green and red peppers. besides its use as a food additive in various spicy cuisines, capsaicin is currently utilized for therapeutic purposes to treat various peripheral painful conditions such as rheumatoid arthritis and diabetic neuropathy. considering consumption of capsaicin as a food additive and its current medicinal application in humans, correct evaluation and precise asses ...19968621114
isolation and characterization of cytosolic copper/zinc superoxide dismutase from capsicum annuum l.a cdna clone for a cytosolic cu/zn superoxide dismutase (cu/znsod) was isolated and characterized from red pepper (capsicum annuum l.). the clone consisted of 735 bp containing one open reading frame (orf) of 459 bp, 46 bp of 5'- and 230 bp of 3'-untranslated region. the nucleotide sequence of the orf showed 93% homology with that of nicotiana plumbaginifolia and tomato. it encodes a polypeptide of 154 amino acids with a molecular weight of 15,300. genomic southern hybridization suggested that o ...19979387156
isocratic non-aqueous reversed-phase high-performance liquid chromatographic separation of capsanthin and capsorubin in red peppers (capsicum annuum l.), paprika and oleoresin.a simple, rapid high-performance liquid chromatography method has been devised in order to separate and quantify the xanthophylls capsorubin and capasanthin present in red pepper (capsicum annuum l.) fruits and preparations made from them (paprika and oleoresin). a reversed-phase isocratic non-aqueous system allows the separation of xanthophylls within a few minutes, with detection at 450 nm, using methyl red as internal standard to locate the various carotenoids and xanthophylls found in plant ...19979025261
partial purification and characterization of red chlorophyll catabolite reductase, a stroma protein involved in chlorophyll breakdown.red chlorophyll (chl) catabolite (rcc) reductase, which catalyzes the reaction of an intermediary chl catabolite (rcc) in the two-step cleavage reaction of pheophorbide (pheide) a into primary fluorescent catabolites (pfccs) during chl breakdown, was characterized and partially purified. rcc reductase activity was present at all stages of barley leaf development and even in roots. the highest specific activity was found in senescent leaves, which were used to purify rcc reductase 1000-fold. amon ...199712223836
chlorophyll breakdown in senescent chloroplasts (cleavage of pheophorbide a in two enzymic steps).the cleavage of pheophorbide (pheide) a into primary fluoescent chlorophyll (chl) catabolites (pfccs) in senescent chloroplasts was investigated. chloroplast preparations isolated from senescent canola (brassica napus) cotyledons exhibited light-dependent production of pfcc when assay mixtures were supplemented with ferredoxin (fd). pfcc production in detergent-solubilized membranes was dependent on the presence of an fd-reducing system. pheide a cleavage required the action of two proteins, phe ...199712223835
recent advances in understanding of vanilloid receptors: a therapeutic target for treatment of pain and inflammation in skin.c-fiber sensory afferent neurons, which contain neuropeptides such as calcitonin-gene related peptide and substance p, mediate a wide variety of physiologic responses, including chemogenic pain, thermoregulation, and neurogenic inflammation. capsaicin, the pungent constituent in red pepper, functions to activate and then, at higher doses and longer times, desensitize this class of neurons. this latter response provides the basis for the therapeutic application of capsaicin. a major advance in th ...19979487017
effect of curcumin and capsaicin on arachidonic acid metabolism and lysosomal enzyme secretion by rat peritoneal macrophages.the inflammatory mediators secreted by macrophages play an important role in autoimmune diseases. spice components, such as curcumin from turmeric and capsaicin from red pepper, are shown to exhibit antiinflammatory properties. the influence of these spice components on arachidonic acid metabolism and secretion of lysosomal enzymes by macrophages was investigated. rat peritoneal macrophages preincubated with 10 microm curcumin or capsaicin for 1 h inhibited the incorporation of arachidonic acid ...19979397403
effect of capsianoside, a diterpene glycoside, on tight-junctional permeability.previous work (hashimoto et al., (1994) biosci. biotech. biochem. 58, 1345) revealed that a sweet pepper extract enhanced the tight-junctional (tj) permeability of a human intestinal caco-2 cell monolayer. in the present study, the substance which modulated the tj permeability was chromatographically purified from the extract. the active substances were identified as capsianosides a-f, diterpene glycosides. treatment of the cells with capsianoside f, the most active compound, decreased the cellu ...19979042350
presence of an acidic glycoprotein in the serum of arthritic rats: modulation by capsaicin and curcumin.levels of various serum proteins were found to change in adjuvant induced arthritis. increased levels of a glycoprotein with an apparent molecular weight of 72 kda (gp a72) were observed in the sera of arthritic rats. gp a72 is an acidic glycoprotein with a pi of 5.1. gp a72 also showed antitryptic activity. the appearance of gp a72 in the serum preceded the onset of paw inflammation in arthritic rats and persisted in the chronic phase. oral administration of the antiinflammatory spice principle ...19979089639
effects of capsaicin on chemically-induced two-stage mouse skin carcinogenesis.capsaicin (trans-8-methyl-n-vanillyl-6-nonenamide) is a principal pungent ingredient of hot red peppers. there are some controversies with regard to its inherent tumorigenicity and mutagenicity. the present work was undertaken to assess tumor initiating and promotional effects of capsaicin in a two-stage mouse skin carcinogenesis model. a single topical application of capsaicin (10 micromol) followed by twice-weekly applications of 12-o-tetradecanoylphorbol-13-acetate onto shaven backs of female ...19979103286
[structural changes in the tissues of white rats after capsaicin administration].tissue structure of albino rat lung, skin and cornea changing after administration of capsaicin (neurotoxin isolated from red pepper) was studied using light and electron microscope. 5 mg/kg dose causes tissue swelling and microcirculatory bed reaction. 200 mg/kg dose leads to more significant dystrophic tissue alterations. fibrosclerosis signs were found in certain cases. microcirculatory disorders are proposed as the main reason for tissue structure alterations observed, although the mechanism ...19979244551
resistance to tobamoviruses in transgenic tobacco plants expressing the coat protein gene of pepper mild mottle virus (korean isolate).red pepper, one of the most important vegetable crops in korea, is severely affected by viral diseases causing 20-50% reduction in product yield. a pepper strain of tobacco mosaic virus (tmv-p) is the most common virus in red pepper. to study the molecular structure of the tmv-p virus, we generated cdna clones of the viral genome. partial sequencing of a few cdna clones revealed that tmv-p shares a 98% identity at the nucleotide level with the spanish isolate of pepper mild mottle virus (pmmv-s) ...19979264016
antibacterial activity of selected fatty acids and essential oils against six meat spoilage organisms.the antibacterial activity of selected fatty acids and essential oils was examined against two gram-negative (pseudomonas fluorescens and serratia liquefaciens) and four gram-positive (brochothrix thermosphacta, carnobacterium piscicola, lactobacillus curvatus, and lactobacillus sake) bacteria involved in meat spoilage. various amounts of each preservative were added to brain heart infusion or mrs (deman, rogosa and sharpe) agars, and the minimum inhibitory concentration was determined for each ...19979310850
food intake, energy balance and body weight control.obesity is a multifactorial and complex affectation that is characterized by a long-term excess energy intake (ei) above energy expenditure (ee). since fat oxidation seems to be dependent on sns activation and also seems to remain acutely unaffected by fat intake, this macronutrient is certainly partly responsible for this situation. in addition, high-fat intake does not induce as potent satiety signals or a compensation effect on subsequent ei as do diets rich in carbohydrates or proteins. more ...19979426360
increase in swimming endurance capacity of mice by capsaicin-induced adrenal catecholamine secretion.increase in endurance swimming capacity caused by capsaicin (cap), a pungent component of red pepper, -induced increase of fat metabolism in mice was investigated using an adjustable-current water pool. the mice administered cap via a stomach tube, showed longer swimming time until exhaustion than the control group of mice, in a dose-dependent manner. the maximal effect was observed at a dose of 10 mg/kg while more than 15 mg/kg had no effect. the increase of endurance was observed only when cap ...19979362118
cross-reactivity between natural rubber latex and food allergens.immediate-type hypersensitivity to latex is a growing problem, especially among health care workers (hcws) and patients requiring long-term catheterization and multiple operations. the responsible allergens are latex proteins, which are found in raw latex, as well as in various latex-containing products. more than 200 polypeptides can be discerned in latex sap and of these, 60 proteins showed reactivity with ige antibodies from patients with latex allergy. several of these proteins have been cha ...199721781817
intranasal capsaicin is efficacious in non-allergic, non-infectious perennial rhinitis. a placebo-controlled study.several authors described capsaicin, the pungent substance in red pepper, as an efficacious therapy for non-allergic non-infectious perennial rhinitis (naniper). repeated capsaicin application induces peptide depletion and specific degeneration of the unmyelinated sensory c-fibres in the nasal mucosa.19979249272
peppers and pain. the promise of capsaicin.capsaicin, the most pungent ingredient in red peppers, has been used for centuries to remedy pain. recently, its role has come under reinvestigation due to evidence that the drug acts selectively on a subpopulation of primary sensory neurons with a nociceptive function. these neurons, besides generating pain sensations, participate through an antidromic activation in the process known as neurogenic inflammation. the first exposure to capsaicin intensely activates these neurons in both senses (or ...19979179523
dietary red pepper ingestion increases carbohydrate oxidation at rest and during exercise in runners.the effects of dietary hot red pepper on energy metabolism at rest and during exercise were examined in long distance male runners 18-23 yr of age. a standardized meal was given on the evening prior to the experiment. the subjects had a meal (2720 kj) with or without 10 g of hot red pepper for breakfast. during rest (2.5 h after meal) and exercise (pedaling for 1 h at 150 w, about 60% vo2max, using cycling ergometry), expired gasses and venous blood were collected. the meal with hot red pepper s ...19979139174
effects of red pepper added to high-fat and high-carbohydrate meals on energy metabolism and substrate utilization in japanese women.the effects of dietary red pepper added to high-fat (hf) and high-carbohydrate (hc) meals on energy metabolism were examined in thirteen japanese female subjects. after ingesting a standardized dinner on the previous evening, the subjects took an experimental breakfast (1883 kj) under the following four conditions: hf meal, hf and red-pepper (10 g) meal, hc meal, or hc and red-pepper meal. palatability of the experimental meals was measured immediately after the meals. expired air was collected ...199810211048
cross-reactivity between latex and sweet pepper due to prohevein. 19989821486
[is there a correlation between dietary habits and hemorrhoidal disease?].it is empirically accepted that certain foods play a role in the pathogenesis of hemorrhoids or their acute exacerbation. the aim of this work was to determine whether there is a relationship between hemorrhoids and certain food-related or common toxin-related factors.19989767961
effect of capsaicin-containing red pepper sauce suspension on upper gastrointestinal motility in healthy volunteers.afferent nerves play a major role in the regulation of gastrointestinal motility. the questions remains if specific food ingredients can selectively activate such fibers. the aim of the study was to investigate the effect of intraesophageal application of a capsaicin-containing red pepper sauce (tabasco) suspension on upper gastrointestinal motility in a controlled trial. after a baseline recording [esophageal motility, balloon distension, electrogastrogram (egg)], red pepper or saline solution ...19989635602
effect of pepper and bismuth subsalicylate on gastric pain and surface hydrophobicity in the rat.the mechanism by which dietary pepper causes dyspepsia and epigastric pain is poorly understood, as is the ability of bismuth subsalicylate (bss) to relieve these symptoms.19989663730
lack of tumor promoting activity of capsaicin, a principal pungent ingredient of red pepper, in mouse skin carcinogenesis.capsaicin (trans-8-methyl-n-vanillyl-6-nonenamide) is the major pungent principle of hot peppers of the genus capsicum. there have been numerous investigations to evaluate the effects of capsaicin on experimental carcinogenesis and mutagenesis, but the results are discordant. in the present study, we have assessed the tumor promoting potential of capsaicin using a two stage mouse skin carcinogenesis model. repeated applications of capsaicin (10 mumol) onto the shaven backs of female icr mice fol ...19989891468
protective effects of fruits and vegetables against in vivo clastogenicity of cyclosphosphamide or benzo[a]pyrene in mice.seven fruits and 10 vegetables commonly consumed in germany were investigated for their anticlastogenic potencies against cyclophosphamide (cp) and benzo[a]pyrene (bap) in the in vivo mouse bone marrow micronucleus assay. we detected protective effects in 76.5% and 70.6% of the samples, respectively, and more or less distinct quantitative differences between the various plant materials and the two clastogens investigated. with respect to cp, moderate activities were exerted by sweet cherries, st ...19989734714
inhibition of listeria monocytogenes and aeromonas hydrophila by plant extracts in refrigerated cooked beef.refrigerated ready-to-eat foods are becoming increasingly popular but are often vulnerable to contamination and subsequent growth by psychrotrophic foodborne pathogens. consequently, there is a need for additional methods to assure the safety of these foods. beef slices prepared from roasted whole sirloin tips were used in the study. nine plant extracts were evaluated for ability to inhibit the growth of two psychrotrophic pathogens (aeromonas hydrophila and listeria monocytogenes) in refrigerat ...19989708301
chemoprotective properties of some pungent ingredients present in red pepper and ginger.there has been a substantial body of data, supporting that dietary factors have a profound impact on prevention as well as etiology of human cancer. capsaicin has been tested by many investigators for its effects on experimental carcinogenesis and mutagenesis. data in the literature indicate that capsaicin has dual effects on carcinogenic and mutagenic processes. at present, there is no solid evidence that hot red and chili peppers or their principal pungent ingredient capsaicin are carcinogenic ...19989675305
in vitro antimutagenic and in vivo anticlastogenic effects of carotenoids and solvent extracts from fruits and vegetables rich in carotenoids.the water insoluble residues of some carotenoid-rich fruits and vegetables, such as apricots, oranges, brussels sprouts, carrots, yellow-red peppers, and tomatoes, were sequentially extracted with n-hexane, dichloromethane, acetone, and 2-propanol, and solvent extracted materials were tested for inhibition of mutagenicities induced by aflatoxin b1 (afb1), benzo[a]pyrene (bap), 2-amino-3-methylimidazo[4,5-f]quinoline (iq), and cyclophosphamide (cp) in histidine-deficient strains of salmonella typ ...19989639691
distribution and diversity of geminiviruses in trinidad and tobago.abstract seven crop and eight weed species from 12 agricultural locations in trinidad and tobago were assayed for the presence of whitefly-transmitted geminiviruses (wtgs) by using dot blot hybridization and polymerase chain reaction (pcr) amplification of the n-terminal coat protein sequence with degenerate primers. the amplified fragments were cloned and analyzed by restriction enzyme digestion to determine fragment length polymorphism among the cloned fragments. representative clones were the ...199818944827
vegetable and fruit consumption and prostate cancer risk: a cohort study in the netherlands.the association between 21 vegetables and eight fruits and prostate cancer risk was assessed in the netherlands cohort study among 58,279 men of ages 55-69 years at baseline in 1986. after 6.3 years of follow-up, 610 cases with complete vegetable data and 642 cases with complete fruit data were available for analysis. in multivariate case-cohort analyses, the following rate ratios (rrs) and 95% confidence intervals (cis) for vegetable consumption were found (comparing highest versus lowest quint ...19989718219
study of lability and kinetics of the main carotenoid pigments of red pepper in the de-esterification reaction.different concentrations of potassium hydroxide in methanol were used to control the time required for complete de-esterification of the carotenoids present in paprika oleoresin, and the effectiveness of the overall process was determined. with concentrated solutions of potash, 20% and 30% (w/v), the reaction was completed in a shorter time but the overall process was less effective. solutions of low concentration, 5% and 10% (w/v), enabled the kinetic study to be carried out and the rates of de ...199810554278
determinants of salt use in cooked meals in the netherlands: attitudes and practices of food preparers.to assess current habits of using salt and other seasonings in food preparation, and to investigate the psychosocial determinants of salt use. in addition we examined differences in salt consumption and psychosocial determinants with respect to stages of change and socio-economic classes.199910369495
isolation and characterization of mitochondrial manganese superoxide dismutase (mnsod) from capsicum annuum l.a cdna clone for a mitochondrial manganese superoxide dismutase (mnsod) was isolated and characterized from red pepper (capsicum annuum l.). the clone consisted of 941 bp containing one open reading frame (orf) of 687 bp, 34 bp/220 bp of 5'/3'-untranslated region. amino acid sequence of the orf showed the highest homology (86%) with that of nicotiana plumbaginifolia. it encodes for a polypeptide of 228 amino acids with a molecular mass of 25.5 kda and a pi value of 8.39. genomic southern hybridi ...199910672929
change in the carotenoid and antioxidant content of spice red pepper (paprika) as a function of ripening and some technological factors.a study was conducted to investigate the change in quality attributes of red pepper (paprika) (capsicum annuum l. var. km-622) as a function of ripening and some technological factors. of quality attributes, carotenoids and bioantioxidants (ascorbic acid and tocopherols) have been studied. it was found that the dynamics of fruit ripening with regard to carotenoids and bioantioxidants was influenced to a considerable extent by weather conditions of the production season. a rainy and cool season y ...199910563856
9-oxooctadeca-10,12-dienoic acids as acetyl-coa carboxylase inhibitors from red pepper (capsicum annuum l.).a methanol extract of red pepper showed potent acetyl-coa carboxylase inhibitory activity. the active principles were isolated and identified as (e, e)- and (e, z)-9-oxooctadeca-10,12-dienoic acids by instrumental analyses. the ic50 values of the compounds were 1.4×10(-6) and 1.5×10(-6) m, respectively, their activity being nearly sixty-times higher than that of the common fatty acids themselves. a comparative study of the structure-activity relationship among their related compounds showed that ...199927393256
renal lesions in streptozotocin-induced diabetic rats maintained on onion and capsaicin containing diets.onion (allium cepa) powder and capsaicin, the pungent principle of red pepper (capsicum annum) were added in the amounts of 3 g% and 15 mg%, respectively, to the diet of streptozotocin-induced diabetic wistar rats for 8 weeks. the presence of renal lesions was assessed by the extent and quality of proteinuria and by the leaching of renal tubular enzymes into the urine. renal integrity was assessed by measuring the activities in the kidney tissue of several key enzymes of carbohydrate metabolism ...199915539326
nutritional quality and the presence of anti-nutritional factors in leaf protein concentrates (lpc).leaf protein concentrates were prepared from 25 different plant species. the concentrates contained acceptable levels of all essential amino acids; except methionine which was limiting with chemical scores ranging between 13.5-96%. lpcs from sesbania, nebergrass, lufa and sweet pepper were lethal to the rats. per ranged between 0.28 for safflower lpc up to 1.95 for rape. positive nitrogen balance was obtained with diets based on lpcs with mean values ranging between 6.4 and 22.42 mg n per day. t ...199910719564
evaluation of the efficacy of the crude extracts of capsicum frutescens, citrus limon and opuntia vulgaris against newcastle disease in domestic fowl in tanzania.prophylactic and therapeutic efficacy of a combination of capsicum frutescens (red pepper), citrus limon (lemon) and opuntia vulgaris (prickly pear) against newcastle disease (nd) in domestic fowl were evaluated. eighty-eight broiler chickens were divided into five groups. birds from three groups were inoculated with velogenic nd virus strain, whereas birds from two groups were left as controls. two groups received a mixture of the plant extract three days prior to inoculation and birds from one ...199910624862
lipid peroxidation and the oxidative burst associated with infection of capsicum annuum by botrytis cinereaa combination of electron paramagnetic resonance (epr) spectroscopy and analytical chemistry has been used to study the changes in free radical content, transition metal ion status and lipid peroxidation following inoculation of fruits of sweet pepper (capsicum annuum) with botrytis cinerea. epr detected a high concentration of an unidentified free radical associated with the spreading lesion that extends into the surrounding, healthy tissues. in addition, the epr-detectable iron(iii) was highes ...199910607300
effects of roasting on pyrazine contents and oxidative stability of red pepper seed oil prior to its extraction.red pepper seeds were roasted with constant stirring for 6, 9, 10, and 12 min at 210 degrees c, and oils were extracted from the roasted red pepper seeds using an expeller. the iodine values and fatty acid compositions of red pepper seed oils did not change with roasting time. the fatty acid composition of the oil obtained from the red pepper seeds roasted for 6 min was 0.24% myristic acid, 13. 42% palmitic acid, 0.33% palmitoleic acid, 2.07% stearic acid, 10. 18% oleic acid, 73.89% linoleic aci ...199910564041
capsaicin induces cystatin s-like substances in submandibular saliva of the rat.irritating dietary substances such as tannin and papain have been reported to alter the morphology of salivary glands and their secretions. such alterations can be one line of protection from toxic or irritating substances in food. we investigated the effects of dietary capsaicin (a pungent ingredient of hot red pepper) on the rat submandibular gland and its secretions. several groups of animals were offered either control diets or diets containing capsaicin (from 0.0001 to 0.1%) for seven days. ...199910520965
modulation of intestinal functions by food substances.the small intestinal epithelium plays a crucial role in the digestion/modification of food components, absorption of nutrients and recognition of food-derived signals. it also acts as a barrier against unfavourable materials in food. these intestinal functions may be influenced by food substances. in this paper, the effects of various food substances on the intestinal functions, particularly the absorption functions, are discussed. a new assay method, using a monolayer culture system of human in ...199910399347
[effect of green vegetable on digestive tract absorption of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans in rats].the effect green vegetable on fecal excretion of polychlorinated dibenzo-p-dioxin (pcdd) congeners and polychlorinated dibenzofuran (pcdf) congeners was examined in male rats. the rats were administered 10% vegetable diets or a basal diet containing 0.2 ml of the causal rice-bran oil of yusho that had occurred in the southwest part of japan in 1968 and kept on the same diet for five days. the fecal excretion of 2,3,7,8-t4cdd and 2,3,4,7,8-p5cdf in the group fed with komatsuna, mitsuba, spinach a ...199910396873
2,4-nonadienal and benzaldehyde bioantimutagens in fushimi sweet pepper (fushimi-togarashi).fushimi sweet pepper, "fushimi-togarashi", is one of the "kyo-yasai", traditional vegetables, in kyoto, japan. the chloroform fraction of fushimi sweet pepper showed bioantimutagenicity on uv induced mutation in escherichia coli b/r wp2. the bioantimutagen was purified with silica gel chromatography and identified as 2, 4-nonadienal (id(50) = 20 microg/plate) on the basis of gc retention time and ei-ms spectrum of authentic 2,4-nonadienal. the sweet pepper also contained a known bioantimutagen, ...199910563930
analgesic effect of capsaicin in idiopathic trigeminal neuralgia.twelve informed and consenting patients were studied to determine the influence of capsaicin, the pungent component of the red pepper, on trigeminal neuralgia. all of these patients had idiopathic trigeminal neuralgia. these patients were followed up for 1 yr after the topical application over the painful area of 1.0 g of capsaicin three times a day for several days. six patients had complete and four patients had partial relief of pain; the remaining two patients had no relief of pain. of the 1 ...19991539818
effects of red pepper on appetite and energy intake.two studies were conducted to investigate the effects of red pepper (capsaicin) on feeding behaviour and energy intake. in the first study, the effects of dietary red pepper added to high-fat (hf) and high-carbohydrate (hc) meals on subsequent energy and macronutrient intakes were examined in thirteen japanese female subjects. after the ingestion of a standardized dinner on the previous evening, the subjects ate an experimental breakfast (1883 kj) of one of the following four types: (1) hf; (2) ...199910743483
latex type i sensitization and allergy in children with atopic dermatitis. evaluation of cross-reactivity to some foods.recent studies have demonstrated that allergy to natural rubber latex (nrl) is associated with cross-reactivity to certain foods. the aim of this study was to investigate the prevalence of nrl sensitization and allergy in children with atopic dermatitis (n=74). we also examined cross-reactions between latex and foods, and compared the frequency of suspected latex cross-reacting fruits in children with and without nrl-specific immunoglobulin e (ige). twelve of the 74 atopic children studied (16.2 ...199910565556
estimation of precision and efficiency mass transfer steps for the determination of pesticides in vegetables aiming at the expression of results with reliable uncertainty.a 'bottom-up' approach for the expression of results obtained from analytical methods that include analytical steps with recovery inherently different from 100% [mass transfer steps (mts): extraction, evaporation, clean-up procedures, digestion, etc.] is presented. the estimation of the combination of all mts uncertainty involves the comparison of the experimental dispersion of replicated analyses of spiked samples with the estimation of the uncertainty obtained for the combination of all uncert ...200011002928
[topical administration of capsaicin in dermatology for treatment of itching and pain].treatment of pruritus as observed in many dermatological and internal diseases may be very often disappointing. there are many reports describing the topical use of capsaicin, the pungent agent in red pepper. long term administration of capsaicin depletes neuropeptides in unmyelinated, polymodal c-type and small myelinated a delta-type cutaneous nerves that conduct pruritus and pain.200010789077
effects of capsaicin-containing yellow curry sauce on sympathetic nervous system activity and diet-induced thermogenesis in lean and obese young women.the present study was designed to investigate the effect of capsaicin, a pungent component of red pepper, on the sympathetic nervous system (sns) activity and energy metabolism in 16 age- and height-matched lean and obese young women. the sympatho-vagal activities were assessed by means of spectral analysis of heart rate variability (hrv) during the resting condition and after the meal (2,016 kj) with capsaicin (3 mg). energy expenditure was also measured under the two conditions. there was no s ...200011227803
[fermentation of the pimento used for stuffing green olives]. 20005083210
influence of intragastric perfusion of aqueous spice extracts on acid secretion in anesthetized albino rats.the effect of spices on gastric acid secretion is variable. their mechanism of action is also not well established.200010812814
enzymatic hydrolysis of carotenoid fatty acid esters of red pepper (capsicum annuum l.) by a lipase from candida rugosa.analyses of red pepper extracts which had been pretreated with lipase type vii (ec 3.1.1.3.) from candida rugosa showed for the first time pepper carotenoid esters to be substrates of this enzyme. however, the extent of enzymatic hydrolysis depends on the respective carotenoid and was not quantitative compared to chemical saponification. after enzymatic cleavage, 67-89% of total capsanthin, 61-65% of total zeaxanthin, 70-81% of total beta-cryptoxanthin and 70-86% of total violaxanthin were detec ...200011204204
inhibitory effects of curcumin and capsaicin on phorbol ester-induced activation of eukaryotic transcription factors, nf-kappab and ap-1.recently, considerable attention has been focused on identifying dietary and medicinal phytochemicals that can inhibit, retard or reverse the multi-stage carcinogenesis. spices and herbs contain phenolic substances with potent antioxidative and chemopreventive properties. curcumin, a yellow colouring agent from turmeric and capsaicin, a pungent principle of red pepper exhibit profound anticarcinogenic and antimutagenic activities. two well-defined eukaryotic transcription factors, nuclear factor ...200011216470
dna repair effect of traditional sweet pepper fushimi-togarashi: seen in suppression of uv-induced cyclobutane pyrimidine dimer in human fibroblast.the aqueous fraction of fushimi sweet pepper increased the repair effect of the solvent control against uv-induced cyclobutane pyrimidine dimers in human fibroblast to 150%, but ordinary sweet pepper did not have a statistically significant effect. when fushimi sweet pepper was boiled, the activity of the aqueous fraction was elevated to 209% of the control (p<0.05), while that of the grilled state was decreased to 125% of the control. the repair activity of a dialyzate (mw<12,000) of the aqueou ...200011210119
cdna cloning and characterization of a plant protein that may be associated with the harpinpss-mediated hypersensitive response.hypersensitive response-assisting protein (hrap) is a novel plant protein that can intensify the harpinpss-mediated hypersensitive response (hr) in harpinpss-insensitive plants, such as the vegetative stage of sweet pepper. in this report, we identified a hrap cdna clone from sweet pepper (capsicum annuum cv. ecw). the sequence of this cdna clone showed no appreciable similarity to any other known sequences. however, it contained three positively charged regions, a typical signal peptide and a c ...200011052195
carotenoid biosynthesis changes in five red pepper (capsicum annuum l.) cultivars during ripening. cultivar selection for breeding.changes in the biosynthesis of individual carotenoid pigments have been investigated during fruit ripening of five cultivars of red pepper (capsicum annuum l.): mana, numex, belrubi, delfin, and negral (a chlorophyll-retaining mutant when ripe). the study was carried out throughout the ripening process, and with special emphasis on the ripe stage, to discover possible differences between cultivars and to characterize these by their carotenoid pattern and content for selecting the best varieties ...200010995282
formation of volatile compounds during heating of spice paprika (capsicum annuum) powder.spice paprika (red pepper; capsicum annuum) is the most cultivated spice worldwide and is used mainly for its color and pungency. however, current research is also focusing on the flavor as an important parameter. this paper deals with the kinetics of the formation of those volatiles that indicate a decrease in spice paprika quality due to maillard reaction, hydrolytic reactions, and oxidative degradation reactions of lipids such as fatty acids and carotenoids. spice paprika volatiles were quant ...200010888567
[mycotoxins in spices].since antiquity, spices have been used for flavoring foods and beverages and for medication. they play an important role in the national economy of several of the producing, exporting and importing countries. tropical climatic conditions under which spices are grown offer a favorable environment for the fungal and mycotoxin contamination. this review summarized information from 44 reports on the occurrence of mycotoxins (aflatoxins, ochratoxins zearalenone, fumonisins, trichothecenes) and their ...200011452374
rapid spectrophotometric determination of red and yellow isochromic carotenoid fractions in paprika and red pepper oleoresins.a rapid method has been developed for the determination of the red (r) and yellow (y) isochromic carotenoid pigments fractions in paprika and oleoresins, based on uv-visible spectrophotometric measurement at two characteristic wavelengths and application of the lambert-beer law for multicomponent mixtures. the wavelengths 472 and 508 nm were selected as the most appropriate for simultaneous quantification of these fractions in the acetone extract of pigments. experimental determination of the sp ...200111513632
occurrence of the toxigenic fungi (producers of aflatoxins and ochratoxin a) in foodstuffs in the czech republic 1999-2000.the occurrence of toxigenic fungi producing aflatoxins and ochratoxin a in foodstuffs was studied in the czech republic. twenty five commodities were collected at twelve collection places in the czech republic (300 food samples). the presence of potentially toxigenicaspergillus flavus was observed in 28% of the sampled foods (black pepper, caraway seeds, fruit tea, black tea, oat flakes, fine flour, rolled oat flakes and semolina) in the year 1999, and in 25% of the sampled foods (black pepper, ...200123605869
failure of capsaicin-containing red pepper sauce suspension to induce esophageal motility response in patients with barrett's esophagus.the physiologic importance of afferent sensory pathways in the esophageal motor functions has been recently recognised. capsaicin-sensitive sensory afferents were shown to play a role in the maintenance of mucosal integrity of the gi tract, and regulation of human esophageal motility. the aim of this study was to investigate the effect of topical application of capsaicin-containing red pepper sauce (tabasco, 25%v/v, ph:7.0) suspension on the phasic activity of the human esophagus of healthy volu ...200111595437
botrydial is produced in plant tissues infected by botrytis cinerea.the fungal metabolite botrydial was detected for the first time in ripe fruits of sweet pepper (capsicum annuum) wound-inoculated with conidial suspensions of botrytis cinerea and also in leaves of phaseolus vulgaris and arabidopsis thaliana inoculated without wounding. this phytotoxin was produced in soft rot regions of the infection. in c. annuum, the most aggressive isolate produced the highest botrydial concentrations in planta. the levels of botrydial produced by this isolate did not correl ...200111397435
biological activity of a fruit vegetable, "anastasia green", a species of sweet pepper.russian green sweet pepper (anastasia green) was successively extracted with hexane, acetone, methanol and 70% methanol and the extracts were further separated into a total of twenty fractions by silica gel or ods column chromatographies. the biological activities of these extracts and fractions were compared. the extracts and fractions showed higher cytotoxic activity against two human oral tumor cell lines than against normal human gingival fibroblasts, suggesting their tumor-specific action. ...200111695243
capsaicin-induced apoptosis in sk-hep-1 hepatocarcinoma cells involves bcl-2 downregulation and caspase-3 activation.hepatocellular carcinoma is one of the most lethal malignancies and there is no effective preventive measure in this highly malignant disease to date. in the present study, we investigated the chemopreventive potential of capsaicin (8-methyl-n- vanillyl-6-nonenamide), the principal pungent ingredient found in hot red pepper, in sk-hep-1 hepatocellular carcinoma cells. treatment of capsaicin inhibited growth of sk-hep-1 cells in a concentration-dependent manner while 4-methoxy capsaicin (met-caps ...200111275362
effect of red peppers (capsicum frutescens) intake during gestation on thermonociceptive response of rat offspring.oral administration of aqueous red pepper (capsicum frutescens, cf) solution and low capsaicin (8-methyl-n-vanillyl-6-nonenamide) doses during gestation produces an increase in the latency of the thermonociceptive escape response of rat offspring. the present work shows that different amounts of cf (10%, 25% and 50%) incorporated to normal food of gestating rats modify in a dose-dependent manner the flexion reflex latency (r), as well as the latency of appearance of antialgesic behaviours expres ...200111165333
capsaicin potentiates 1,25-dihydoxyvitamin d3- and all-trans retinoic acid-induced differentiation of human promyelocytic leukemia hl-60 cells.human promyelocytic leukemia hl-60 cells are differentiated into monocytic or granulocytic lineage when treated with 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] or all-trans retinoic acid, respectively. in this study, the effect of capsaicin, an active component of the red pepper of the genus capsocum, on cell differentiation was investigated in a hl-60 cell culture system. treatment of hl-60 cells with 5-30 microg/ml capsaicin for 72 h inhibited cell proliferation and induced a small increase in ce ...200111408028
transgenic rice plants expressing the ferredoxin-like protein (ap1) from sweet pepper show enhanced resistance to xanthomonas oryzae pv. oryzae.we used particle bombardment to cotransform mature seed-derived rice callus (oryza sativa l., ssp. japonica, cv. eyi 105) with plasmids containing the linked marker genes gusa and hpt, and the ap1 gene encoding an amphipathic protein previously shown to delay the hypersensitive response induced in non-host plants by the pathogen pseudomonas syringae pv. syringae (pss). thirty-two independent lines of transgenic rice plants were regenerated, and 27 of these lines carried all three transgenes as s ...200111297801
alpha-tocopherol content in 62 edible tropical plants.vitamin e was determined by the high-performance liquid chromatography (hplc) method. all the plants tested showed differences in their alpha-tocopherol content and the differences were significant (p < 0.05). the highest alpha-tocopherol content was in sauropus androgynus leaves (426.8 mg/kg edible portion), followed by citrus hystrix leaves (398.3 mg/kg), calamus scipronum (193.8 mg/kg), starfruit leaves averrhoa belimbi (168.3 mg/kg), red pepper capsicum annum (155.4 mg/kg), local celery apiu ...200111410015
growth and aflatoxin production ofaspergillus flavus on some spices marketed in turkey.growth and aflatoxin production byaspergillus flavus (mam-268 c17, tns=98) on autoclaved coconut, black pepper, crushed red pepper, red pepper powder were studied. coconut were found to be a better substrate for fungal growth, as well as aflatoxin production. red pepper powder could be considered as a poor substrate for fungal growth. the amount of aflatoxin found in crushed red pepper under laboratory conditions is much less compared with coconut, black pepper and red pepper powder.200123605610
treatment of prurigo nodularis with topical capsaicin.prurigo nodularis is an eruption of lichenified or excoriated nodules caused by intractable pruritus that is difficult to treat. therefore the antipruritic efficacy of capsaicin seemed to be of particular interest because this alkaloid, extractable from red pepper, interferes with the perception of pruritus and pain by depletion of neuropeptides in small sensory cutaneous nerves.200111209117
combined effects of red pepper and caffeine consumption on 24 h energy balance in subjects given free access to foods.the effects of red pepper and caffeine ingestion on energy and macronutrient balances were examined in eight caucasian male subjects. all subjects participated in two randomly assigned conditions: control and experimental (red pepper and caffeine). after ingesting a standardized breakfast, subjects ate three meals ad libitum (lunch, dinner and breakfast) and snacks which were served approximately 2 h after the lunch and dinner over a 24 h period. two appetizers with or without 3 g red pepper) we ...200111242488
ch-19 sweet, nonpungent cultivar of red pepper, increased body temperature in mice with vanilloid receptors stimulation by capsiate.we investigated the effect of ch-19 sweet, a nonpungent cultivar of red pepper, and capsiate, a nonpungent capsaicin analog found in ch-19 sweet on body temperature in mice. the body temperature was recorded from conscious and unrestrained mice by use of a telemetry system. the body temperature in the mice administered ch-19 sweet was higher than in the mice administered california-wandar, which contains no capsiate or capsaicin. the body temperature in the mice administered capsiate was higher ...200111767210
determination of aflatoxins b1, b2, g1 and g2 in spices using a multifunctional column clean-up.a rapid and simple method using a multifunctional column, which contains lipophilic and charged active sites, was developed to analyse aflatoxins b1, b2, g1 and g2 in various spices, such as red pepper and nutmeg. after extraction by acetonitrile:water (9:1) and clean-up using multisep #228 column, the aflatoxins and aflatoxin-tfa derivatives are determined using lc with fluorescence detection. recoveries of each aflatoxin b1, b2, g1 and g2 spiked to red pepper, white pepper, black pepper, nutme ...200111695861
ch-19 sweet, a non-pungent cultivar of red pepper, increased body temperature and oxygen consumption in humans.we investigated the effect of ch-19 sweet, a non-pungent cultivar of red pepper, on body temperature and oxygen consumption in humans. ch-19 sweet was given to 11 healthy volunteers, and core body temperature, body surface temperature and oxygen consumption were measured. the control group ingested california-wandar, which contained neither capsaicin nor capsiate. the core body temperature in the ch-19 sweet group was significantly higher than that in the control group (p<0.01). the forehead tem ...200111676017
enzymatic synthesis of vanillin.due to increasing interest in natural vanillin, two enzymatic routes for the synthesis of vanillin were developed. the flavoprotein vanillyl alcohol oxidase (vao) acts on a wide range of phenolic compounds and converts both creosol and vanillylamine to vanillin with high yield. the vao-mediated conversion of creosol proceeds via a two-step process in which the initially formed vanillyl alcohol is further oxidized to vanillin. catalysis is limited by the formation of an abortive complex between e ...200111409992
a comparison of sole carbon source utilization patterns and phospholipid fatty acid profiles to detect changes in the root microflora of hydroponically grown crops.sole carbon source utilization (scsu) patterns and phospholipid fatty acid (plfa) profiles were compared with respect to their potential to characterize root-inhabiting microbial communities of hydroponically grown crops. sweet pepper (capsicum annum cv. evident), lettuce (lactuca sativa cv. grand rapids), and four different cultivars of tomato (lycopersicon esculentum cvs. gitana, armada, aromata, and elin) were grown in 1-l black plastic beakers placed in a cultivation chamber with artificial ...200111358169
[rapid analysis of aflatoxins in raw peanuts, corn, buckwheat and red pepper by a new mini-column cleanup and hplc using post-column photochemical derivatization system].a rapid and clean method for the analysis of aflatoxins (afs) was developed by using a new column and post-column photochemical derivatization hplc with fluorescence detection. the new cleanup column consisted of magnesia and basic alumina poured on the top of a commercial multi-functional mini-column. it was extremely effective for the cleanup of afs from raw peanut, corn, buckwheat and red pepper. fluorescent substances, which interfered with the analysis of afs from corn, were completely abso ...200212436711
a modified hplc method for the determination of ochratoxin a by fluorescence detection.a high-performance liquid chromatographic method (hplc) with fluorescent detector is described for the determination of ochratoxin a (ota). a mobile phase consisting of acetonitrile:water:acetic acid (99:99:2, v/v/v) was used for the resolution of the compound on a c(18) hypersil column. the retention time for ota and diflunisal which was used as an internal standard (is) were 11.7 and 12.8 min, respectively. the method is selective, reliable, reproducable with relative standard deviation (rsd) ...200212378560
field and laboratory selection of frankliniella occidentalis (pergande) for resistance to insecticides.response of western flower thrips, frankliniella occidentalis (pergande), to selection for resistance to insecticides commonly used to control this pest in murcia (south-east spain) was studied under field and laboratory conditions. in the field, plots within sweet pepper crops in commercial and experimental greenhouses were treated under different selection strategies: insecticide rotation versus formetanate reiteration, formetanate reiteration versus acrinathrin reiteration, and formetanate re ...200212233182
arsenic, sb and bi contamination of soils, plants, waters and sediments in the vicinity of the dalsung cu-w mine in korea.the objective of this study is to investigate environmental contamination derived from metalliferous mining activities. in the study area, the dalsung cu-w mine, soils, various crop plants, stream waters, sediments and particulates were sampled in and around the mine and analyzed for as, sb and bi by icp-aes with a hydride generator. in addition, soil ph, cation exchange capacity, loss-on-ignition and soil texture were also measured. concentrations of as, sb and bi in surface soils sampled in th ...200212186294
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