Publications

phenotypic characterization of encephalitis in the brains of goats experimentally infected with spanish goat encephalitis virus.	spanish goat encephalitis virus (sgev) is a novel tick-borne flavivirus subtype, closely related to the flavivirus louping ill virus (liv). sgev caused a severe, acute and mortal neurological disease outbreak in northern spain in a goat herd. in order to characterize the cell population in lesions and to determine the distribution of the inflammatory cells, central nervous system (cns) samples of nine female alpine goats challenged subcutaneously with sgev over the right thorax behind the elbow ...	2019	31821945
detection of a novel tick-borne flavivirus and its serological surveillance.	tick-borne encephalitis virus (tbev), a flavivirus that causes severe neurological symptoms in humans, has been found in hokkaido, japan. in the present study, we detected sequences from a novel tick-borne flavivirus, designated yamaguchi virus (ygv), in liver and serum samples obtained from a wild boar in the yamaguchi prefecture, japan. phylogenetic analysis revealed that ygv belongs to the tbev complex and is closely related to langat virus (lgtv). ygv was also detected by specific rt-pcr fro ...	2019	30902589
exposure of wild ungulates to the usutu and tick-borne encephalitis viruses in france in 2009-2014: evidence of undetected flavivirus circulation a decade ago.	abstract: flaviviruses have become increasingly important pathogens in europe over the past few decades. a better understanding of the spatiotemporal distribution of flaviviruses in france is needed to better define risk areas and to gain knowledge of the dynamics of virus transmission cycles. serum samples from 1014 wild boar and 758 roe deer from 16 departments (administrative units) in france collected from 2009 to 2014 were screened for flavivirus antibodies using a competitive elisa (celisa ...	2019	31861683
traf6 plays a proviral role in tick-borne flavivirus infection through interaction with the ns3 protease.	tick-borne flaviviruses (tbfvs) can cause life-threatening encephalitis and hemorrhagic fever. to identify virus-host interactions that may be exploited as therapeutic targets, we analyzed the tbfv polyprotein in silico for antiviral protein-binding motifs. we obtained two putative tumor necrosis factor receptor-associated factor 6 (traf6)-binding motifs (tbms) within the protease domain of the viral nonstructural 3 (ns3) protein. here, we show that tbfv ns3 interacted with traf6 during infectio ...	2019	31129244
trans complementation of replication-defective omsk hemorrhagic fever virus for antiviral study.	omsk hemorrhagic fever virus (ohfv) is a tick-borne flavivirus classified as a biosafety level-4 (bsl4) pathogen. studies of ohfv are restricted to be conducted within bsl4 laboratories. currently, no commercial vaccines or antiviral drugs are available against ohfv infection. in this study, we recovered a replication-deficient ohfv with an ns1 deletion (ohfv-δns1) and reporter virus replacing ns1 with the gaussia luciferase (gluc) (ohfv-δns1-gluc). both the defective ohfv-δns1 and ohfv-δns1-glu ...	2019	30949960
growth kinetics of kyasanur forest disease virus in mammalian cell lines and development of plaque reduction neutralization test.	kyasanur forest disease virus (kfdv) is a tick-borne flavivirus identified in 1957 in the karnataka state of india causing fatalities in monkeys and humans. even after the introduction of a vaccine in the endemic areas, hundreds of cases are reported every year. being a high-risk category pathogen, the studies on this virus in india were limited till the past decade. the growth characteristics of this virus in various mammalian cell lines have not yet been studied. in this study, we have demonst ...	2019	30994413