Publications

in vitro susceptibilities of nocardia species to newer antimicrobial agents.	the in vitro activities of various quinolones, two newer cephalosporins, and imipenem against 23 strains of nocardia asteroides, 4 strains of nocardia brasiliensis, and 4 strains of nocardia caviae were determined by an agar dilution method. pd-117558, a newer carboxyquinolone, and imipenem were the most active agents tested, inhibiting 90% of n. asteroides isolates at an mic of 2 micrograms/ml. of the 23 strains of n. asteroides, 15 were susceptible to cefpirome and 10 were susceptible to cipro ...	1988	3190193
acid phosphatase activity in nocardia brasiliensis and nocardia asteroides.	nocardia asteroides and n. brasiliensis strains were found to possess acid phosphatase activity. this enzyme was found to be cell-associated and its activity paralleled the cell mass increase seen during the nocardia growth cycle. of the strains tested, n. brasiliensis exhibited the highest enzymatic activity. implications of these findings are related to current evidence which indicates that other microbial acid phosphatases may constitute potential pathogenic factors for humans.	1988	3287423
the efficiency of 1 m nh4cl and 2 m nacl for the isolation of pathogenic nocardia from soil.	the present paper reports an improvement to the classical method of the paraffin bait, by the usage of 1 m nh4cl or 2 m nacl to eliminate contaminant microflora of soil. the purpose is to introduce a change in the paraffin bait method in order to reduce time required to isolate pathogenic strains of nocardia from their natural sources. for this study three main criteria were used: a) determination of the inhibitory effect of different concentrations of salts on soil microflora; b) the tolerance ...	1989	2594047
mycetoma due to nocardia brasiliensis: first case report from the union territory of delhi.	the first case of mycetoma caused by nocardia brasiliensis from the union territory of delhi is reported. the patient, a permanent resident of the union territory of delhi, acquired the disease 10 years ago following an accidental injury to his right hand. the diagnosis of the case was made on the basis of demonstration of the grains in direct microscopy, isolation of the organism in culture directly from the grains, identifying the organism by the conventional tests and through histopathology o ...	1989	2608095
cutaneous abscess caused by nocardia brasiliensis: report of a case.	we report a case of cutaneous nocardia brasiliensis infection. the patient had received radiotherapy and anti-neoplastic chemotherapy for epidermoid carcinoma of the left sphenoid sinus with bone destruction. he developed fever and an ulcer on the dorsal medial surface of the left hand after an intravenous infusion of chemotherapeutic agents in the same site 3 days earlier. needle aspiration of the abscess disclosed polymorphonuclear leukocytes, and a partially acid-fast, gram-positive filamento ...	1989	2636252
[surgical treatment of mycetoma located in the face].	the first case described in mexico, was studied by cícero in 1900 and 1911 ocaranza, in sonora, reports some cases, pointing out that the first known case dates back to 1874. in usa gonzález ochoa identifies the causative agent of the cases he studied, as the same identified in usa, but he calls attention to the fact that it has already been described under the name of actinomyces brasiliensis, finally, taxonomically it is included among the nocardias. in mexico the micetoma is caused in 90% of ...	1989	2666793
[in vitro susceptibility of pathogenic nocardia to beta-lactam antibiotics, especially imipenem, a carbapenem antibiotic].	in vitro antibacterial activity of 30 beta-lactam antibiotics including 2 beta-lactamase inhibitors (clavulanic acid and sulbactam) against 2 major pathogenic nocardia, i.e. nocardia asteroides group and nocardia brasiliensis was studied. among the antibiotics tested, a newly developed carbapenem antibiotic, imipenem (ipm), was found to be the most active, followed by oxacephem group antibiotic flomoxef (fmox). ipm exhibited activity against only n. asteroides group (n. asteroides, nocardia farc ...	1989	2695658
physiological and pathogenic characteristics of nocardia brasiliensis isolated from human mycetomas.	a previous analysis of the physiological properties of nocardia brasiliensis strains isolated from soil of tucumán proves that non-pathogenic strains have a different behaviour pattern from the pathogenic strains. in the present paper, 16 nocardia brasiliensis strains isolated from human mycetomas were studied in the same way. the object is to determine if any of the nocardia brasiliensis present in soil can produce human mycetomas. the macro and micromorphological, biochemical (17 tests), physi ...	1989	2747785
[disseminated nocardiosis due to nocardia brasiliensis in an ambulatory patient].	we report the case of a 76-year-old man with generalized nocardiosis. the microbiologic pattern, the different clinical manifestations and the treatment of nocardiosis are discussed in general. in the particular case of our patient the disease manifested itself primarily as a subcutaneous abscess, a metastasis secondary to pulmonary nocardiosis. the disease was caused by a nocardia brasiliensis, which is rarely seen in europe and which does not usually cause a generalized form of nocardiosis.	1989	2756262
comparison of antigens from agents of actinomycetoma by immunodiffusion and electrophoresis procedures.	antigenic extracts were prepared from culture filtrates of the principal agents known to cause actinomycetoma, namely actinomadura madurae, actinomadura pelletieri, nocardia asteroides, nocardia brasiliensis, nocardia otitidis-caviarum, and streptomyces somaliensis. these antigenic preparations were compared by immunodiffusion (id), counterimmunoelectrophoresis (cie), line immunoelectrophoresis (lie) and rocket line immunoelectrophoresis (rlie), with rabbit antisera prepared against each of the ...	1990	2126557
use of partially purified 54-kilodalton antigen for diagnosis of nocardiosis by western blot (immunoblot) assay.	a western blot (immunoblot) assay is presented for the diagnosis of nocardiosis with a specific immunodominant 54-kilodalton (kda) antigen purified from a culture filtrate of nocardia asteroides by immunoaffinity chromatography. the chromatography column was prepared with immunoglobulin g obtained from sera from patients with lepromatous leprosy. unbound solutes consisted of specific, partially purified n. asteroides antigens, primarily a 54-kda band, accompanied by two others of 31 and 62 kda. ...	1990	2179262
cultivation of a nocardioform acid-fast chemoautotrophic bacterium from armadillo tissues infected with mycobacterium leprae.	a nocardioform bacterium was isolated from the spleen tissue of an armadillo infected with m. leprae and easily propagated in pure culture in mineral salt medium supplemented with only simple c and n sources (e.g., liquid paraffin, tetradecane, ammonium salts, urea, asparagine, gelatin, xanthin, hypoxanthin etc.). complex organic substances, e.g., tyrosin, casein, peptone, meat extract, egg proteins, serum, blood, yeast extract as well as medium 199, did not support the growth of this organism. ...	1990	2188909
nocardia brasiliensis peritonitis in a patient with aids.		1990	2220750
comparison of species distribution and antimicrobial susceptibility of aerobic actinomycetes from clinical specimens.	to compare the species distribution and antimicrobial susceptibility of aerobic actinomycetes, we evaluated 366 isolates referred to the centers for disease control from october 1985 through february 1988. we used conventional biochemical tests to identify the various species. four species accounted for 191 (52%) of aerobic actinomycete isolates: nocardia asteroides (98 isolates), actinomadura madurae (42 isolates), streptomyces griseus (28 isolates), and nocardia brasiliensis (23 isolates). spu ...	1990	2237117
pulmonary cryptococcus neoformans and disseminated nocardia brasiliensis in an immunocompromised host. case report.		1990	2342607
nocardia brasiliensis leg ulcer in a child.		1990	2352823
acute primary cutaneous nocardiosis.	an acute localized, purulent skin lesion developed on the right knee of an 8-year-old girl and was subsequently diagnosed as a primary cutaneous nocardia brasiliensis infection. it was soon accompanied by intense lymphangitis and regional adenopathy. the patient had a history of a minor abrasion contaminated by soil at the site of infection 8 days before the infection became evident. the infection was successfully treated with a combination of minocycline and dapsone.	1990	2394860
monoclonal antibodies to nocardia asteroides and nocardia brasiliensis antigens.	nocardia asteroides and nocardia brasiliensis whole-cell extracts were used as antigens to generate monoclonal antibodies (mabs). six stable hybrid cell lines secreting anti-nocardia spp. mabs were obtained. these were characterized by enzyme-linked immunosorbent assay, western blot (immunoblot), and immunofluorescence assay. although all the mabs exhibited different degrees of cross-reactivity with n. asteroides and n. brasiliensis antigens as well as with culture-filtrate antigens from mycobac ...	1990	2405017
nocardia brasiliensis cellulitis in a heart transplant patient.	three months after undergoing heart transplantation, a 55-year-old man presented with n. brasiliensis cellulitis resulting from a splinter wound acquired during yard work. surgical débridement was necessary before the infection responded to medical treatment. although pulmonary nocardiosis is a well-documented complication of immunosuppressive therapy, this is the 1st report of a nocardial infection associated with primary skin involvement in a heart transplant patient.	1990	15227399
beneficial effects of hyperbaric oxygen therapy in nocardia brasiliensis soft-tissue infection.	to report the success of hyperbaric oxygen therapy in the treatment of nocardia brasiliensis mycetoma. we believe this to be only the second report in the medical literature of hyperbaric oxygen therapy used in the therapy of nocardial disease.	1991	1857290
community acquired pneumonia: aetiology and prognostic index evaluation.	a 10 month prospective study of all adults admitted to waikato hospital with community acquired pneumonia was performed to assess aetiology, mortality, hospital stay, and the value of a prognostic index based on that obtained from a british thoracic society study. the 92 patients in the survey had a mean age of 56 (range 13-97) years. a microbiological diagnosis was established in 72%; streptococcus pneumoniae (33%), mycoplasma pneumoniae (18%), and influenza a virus (8%) were the most common mi ...	1991	1907034
disseminated nocardia brasiliensis infection: an unusual complication of immunosuppressive treatment for childhood dermatomyositis.	the use of steroids combined with cytotoxic drugs has increased in the last decade. the concomitant increase of opportunistic infections has contributed significantly to morbidity and mortality of patients treated with immunosuppressive agents. we describe a child with dermatomyositis who developed disseminated nocardia brasiliensis infection while receiving steroids and methotrexate. infectious etiology was established by gram stain. the patient was treated successfully. disseminated nocardia b ...	1991	1941833
acquired resistance of nocardia brasiliensis to clavulanic acid related to a change in beta-lactamase following therapy with amoxicillin-clavulanic acid.	previous studies have demonstrated that nocardia brasiliensis is susceptible to amoxicillin-clavulanic acid and that its beta-lactamases are inhibited in vitro by clavulanic acid. a cardiac transplant patient with disseminated infection caused by n. brasiliensis was treated with this drug combination with good response, but relapsed while still on therapy. the relapse isolate was found to be identical to the initial isolate by using genomic dna restriction fragment patterns obtained by pulsed fi ...	1991	2039203
acid and alkali as aids in differentiation of pathogenic nocardia and for their isolation from clinical specimens.	the tolerance of nocardia brasiliensis, nocardia asteroides and nocardia caviae to different concentrations of acids and alkalis was studied in this work. the purpose was to determine their use: (a) in the differentiation of species; (b) for decontamination of clinical materials during the isolation of pathogenic nocardia. the results showed (1) that both 2 m nh3 and 0.05 m h2so4 solutions are useful for differentiating species. they can also be used as a complementary method for identification. ...	1991	2067559
[epidemiology of mycetoma in mexico: study of 2105 cases].	a survey was carried out in mexico to determine the incidence and epidemiological characteristics of mycetoma. data was collected from a total of 2105 cases of mycetoma throughout a 30 year period (1956-1985), with an average incidence of 70 cases per year. results showed a sex distribution of 76.1% male and 23.9% females. age distribution indicated a 35% between 16 to 30 and 23% between 31 to 40 year old population. most cases occurred in land-workers (60.2%) and in housewives with rural reside ...	1992	1308000
lymphocutaneous nocardia brasiliensis infection acquired from a cat scratch: case report and review.	nocardia brasiliensis is a bacterium that is most commonly found in the soil. traumatic inoculation of n. brasiliensis into the skin is the most typical mode of acquisition of infection due to this organism. to the best of my knowledge, i report the first case of lymphocutaneous n. brasiliensis disease from a penetrating cat scratch of the skin, thereby establishing cats as vehicles for the transmission of this infection. treatment with penicillin produced a rapid resolution of all signs and sym ...	1992	1420688
cutaneous nocardiosis associated with insect bites.	we report the first case of cutaneous nocardiosis associated with insect bites. the patient received these insect bites while camping a few days before he presented to the emergency room. the patient was a healthy twenty-six-year-old man, who was asplenic as a result of a motor vehicle accident. he presented to the emergency room with fever, multiple insect bites to his left lower extremity with pustules, lymphangitis, and left inguinal adenopathy. he was hospitalized but his condition failed to ...	1992	1424798
review of nocardial infections in france 1987 to 1990.	on the basis of the numbers of nocardia strains referred to the national reference center for mycoses and antifungal agents (nrc), institut pasteur, paris, in the period from 1987 to 1990, it was estimated that between 150 and 250 cases of nocardiosis are diagnosed in france each year. a total of 63 clinical isolates were referred to the nrc and identified as nocardia asteroides (66.7%), nocardia farcinica (23.8%), nocardia brasiliensis (3.2%), nocardia otitidiscaviarum (4.8%) and nocardia carne ...	1992	1425729
properties of a partially purified acid phosphatase from pathogenic nocardia brasiliensis.	like many other bacteria, nocardia sp. possess acid phosphatase activity. in n. brasiliensis, a human and animal pathogen, this activity was resolved into two enzyme forms by native gel electrophoresis. one (isozyme i) was partially purified and characterized. it exhibited an estimated molecular weight on sds-page of 50 kda, a ph optimum of 5.2, and a km value of 1.25 mm for p-nitrophenylphosphate. the n. brasiliensis enzyme was stable at 4 degrees c for at least 24 h, but readily inactivated at ...	1992	1435947
the producer and biological activities of so-75r1, a new mutactimycin group antibiotic [corrected].	the producer of so-75r1, a new anthracycline group antibiotic was identified as nocardia brasiliensis. so-75r1 was active against gram-positive bacteria, but not active against gram-negative bacteria or fungi. all tested nocardia brasiliensis strains as well as the producer itself were resistant to so-75r1, although four other pathogenic nocardia, i.e. n. asteroides, n. nova, n. farcinica and n. otitidiscaviarum were sensitive [corrected].	1992	1490875
nocardia brasiliensis meningitis.		1992	1494536
tumor necrosis factor and macrophage activation are important in clearance of nocardia brasiliensis from the livers and spleens of mice.	the roles of tumor necrosis factor (tnf) and macrophage activation in clearance of nocardia brasiliensis from balb/c mouse livers and spleens were evaluated. tnf activity was detectable in sera from animals at all stages of infection. treatment of infected mice with an antiserum against tnf significantly enhanced the experimental infection as judged by enumeration of cfu in the spleens and livers of infected mice. in another set of experiments, a population of activated macrophages from the peri ...	1992	1500164
sf2457, a new antibiotic related to amicetin.	a novel nucleoside antibiotic, sf2457, was isolated from the fermentation broth of nocardia brasiliensis sf2457. the structure of sf2457 was determined by degradation studies using alkaline hydrolysis and methanolysis. sf2457 is closely related to the amicetin group antibiotics. the antibiotic exhibited inhibitory activity against gram-positive and gram-negative bacteria.	1992	1500349
so-75r1, a new mutactimycin derivative produced by nocardia brasiliensis.		1992	1500368
in-vitro activity of new carbapenem antibiotics: comparative studies with meropenem, l-627 and imipenem against pathogenic nocardia spp.	mics of two new carbapenems, meropenem and l-627, and imipenem were determined against 98 strains of the nocardia asteroides group (i.e. n. asteroides sensu stricto, nocardia farcinica and nocardia nova), 46 strains of nocardia brasiliensis and 17 strains of nocardia otitidiscaviarum. meropenem and l-627 were less active against the n. asteroides group than imipenem. among the three species of the n. asteroides group, n. nova was the most sensitive to all the carbapenems. meropenem was more acti ...	1992	1506332
disseminated nocardia brasiliensis infection with septic arthritis.	an unusual case of disseminated nocardia brasiliensis infection is presented. the patient, who had been receiving chronic dexamethasone therapy for 4 years, had pneumonia and septic arthritis of the left knee due to n. brasiliensis. to our knowledge, this is the first report from the united states of a synovial joint infection with this organism. disseminated disease due to n. brasiliensis is infrequently reported; it is most often seen in the immunocompromised patient and is often unresponsive ...	1992	1520795
preseptal cellulitis caused by nocardia brasiliensis.		1992	1524136
mini-mycetoma due to nocardia brasiliensis.		1992	1568813
nocardia brasiliensis infection in patients with aids.		1992	1576299
antibody response to nocardia brasiliensis antigens in man.	a crude extract from n. brasiliensis cells grown in brain heart infusion culture was analyzed. it showed a complex mixture of at least 37 bands when resolved with the discontinuous buffer system of laemmli in a gradient sds-page. western blot analysis of 16 sera from n. brasiliensis-infected individuals always showed the recognition of six bands of 61, 49, 45, 42, 26, and 24 kilodaltons (kda). some other bands also reacted but with less intensity. sera from tuberculosis and leprosy patients reac ...	1992	1576408
isolation and purification of two immunodominant antigens from nocardia brasiliensis.	two immunogenic proteins from a crude extract of nocardia brasiliensis were purified to homogeneity. a 61-kda protein (p61) was isolated from a 50% ammonium sulfate precipitate in two steps. initially, p61 was obtained by electroelution in a 10% nondenatured preparative polyacrylamide gel electrophoresis (page). in a second step, the eluate from the nondenatured gel was run in a 12% sodium dodecyl sulfate (sds) preparative polyacrylamide gel. after elution, a single band was demonstrated by sds- ...	1992	1583118
nocardia brasiliensis soft tissue infection in an immunocompromised host.		1992	1641216
enzyme-linked immunosorbent assay for serological diagnosis of nocardia brasiliensis and clinical correlation with mycetoma infections.	we previously identified three immunodominant antigens obtained from a nocardia brasiliensis cell extract and recognized by sera from mycetoma patients (m. c. salinas-carmona, l. vera, o. welsh, and m. rodríguez, zentralbl. bakteriol. 276:390-397, 1992). in the present work, we obtained a crude extract from a mass culture of n. brasiliensis hujeg-1 and purified two immunodominant antigens, the 26- and 24-kda proteins, by using simple physiochemical techniques. with these antigens, we developed a ...	1993	8263174
cervicofacial nocardiosis caused by nocardia brasiliensis in an adult.		1993	8286649
inactivation of rifampin by nocardia brasiliensis.	rifampin was glycosylated by a pathogenic species of nocardia, i.e., nocardia brasiliensis. the structures of two glycosylated compounds (rip-1 and rip-2) isolated from the culture broth of the bacterium were determined to be 3-formyl-23-(o-[beta-d-glucopyranosyl])rifamycin sv and 23-(o-[beta-d-glucopyranosyl])rifampin, respectively. both compounds lacked antimicrobial activity against other gram-positive bacteria as well as the nocardia species.	1993	8328779
pc-766b, a new macrolide antibiotic produced by nocardia brasiliensis. ii. isolation, physico-chemical properties and structure elucidation.	a new macrolide antibiotic, pc-766b, was isolated from the cells of nocardia brasiliensis sc-4710 by acetone extraction, and purified by gel filtration, silica gel chromatography, hplc and tlc. the structure of pc-766b was determined by nmr spectral analysis to be a new class of the hygrolidin family antibiotics. pc-766b had a 16-membered macrocyclic lactone ring, a 6-membered hemiketal ring and a 2-deoxy-d-rhamnose moiety. dl-alpha-tocopherol, known as an antioxidant agent, significantly improv ...	1993	8360110
mycetoma: a retrospective study of 41 cases seen in são paulo, brazil, from 1978 to 1989.	clinical and epidemiological aspects of 41 cases of mycetoma seen in são paulo city from january 1978 to december 1989 are presented. twenty-eight cases (68%) were caused by actinomycetes, while 13 (32%) were caused by true fungi. in only 22 (78%) actinomycetoma cases was it possible to identify the agent. nocardia brasiliensis, by far the commonest actinomycete isolated, was responsible for 13 cases. among eumycetomata, madurella grisea was isolated from 3 cases, scedosporium apiospermum from 2 ...	1993	8366881
pc-766b, a new macrolide antibiotic produced by nocardia brasiliensis. i. taxonomy, fermentation and biological activity.	an actinomycete strain sc-4710, a new soil isolate, was found to produce a new macrolide antibiotic, pc-766b. chemotaxonomic analysis of the producing organism revealed that the cells of sc-4710 had type iv cell wall, type a whole cell sugar pattern, type pii phospholipids, menaquinone mk-8(h4), cellular fatty acids comprising straight-chain saturated, unsaturated and tuberculostearic acids, and mycolic acids. the strain was identified as nocardia brasiliensis (lindenberg) pinoy. the antibiotic, ...	1993	8393851
amoxicillin and clavulanic acid in the treatment of actinomycetoma.	mycetoma is a relatively frequent disease in tropical countries. drugs commonly used need a long period of treatment, and some cases are resistant to these drugs, especially those with bone or visceral involvement. the combination of amoxicillin-clavulanic acid has shown effectiveness against strains of nocardia brasiliensis in vitro.	1993	8444539
nocardia brasiliensis liver abscesses in an aids patient: imaging findings.		1993	8456695
nodular lymphangitis: a distinctive but often unrecognized syndrome.	to describe nodular lymphangitis by reviewing the clinical and epidemiologic features of this disease with an emphasis on distinguishing specific etiologic agents.	1993	8480962
treatment of a nocardia brasiliensis mycetoma with sulfamethoxazole and trimethoprim, amikacin, and amoxicillin and clavulanate.		1993	8481016
nocardiosis in renal transplant recipients undergoing immunosuppression with cyclosporine.	nine cases of nocardiosis were diagnosed among 1,255 renal transplant recipients given cyclosporine (csa)-prednisone for immunosuppression between august 1980 and march 1992 (incidence, 0.7%). of these nine patients presenting with nocardiosis 32-1,806 days after transplantation, eight had pulmonary involvement, two had skin manifestations (one with localized disease), and one had a cerebral abscess and a pleural effusion. all cases required aggressive diagnostic procedures. nocardia asteroides ...	1993	8513056
lymphocutaneous nocardiosis with multiple subcutaneous nodules distributed over the extensor aspect of the forearm. report of a case.	we report a case of lymphocutaneous nocardiosis due to nocardia brasiliensis, marked by multiple subcutaneous nodules and abscesses on the extensor aspect of the left forearm in a 53-year-old woman with systemic lupus erythematosus. nine months before she was first examined, she had fallen on a concrete surface. a nodule had appeared 2 months later at the same site and had expanded and been followed by others, which finally covered most of the extensor aspect of the forearm. we speculated that t ...	1994	7701876
three new reduced anthracycline related compounds from pathogenic nocardia brasiliensis.	three new metabolites were isolated from a pathogenic bacterium, nocardia brasiliensis ifm 0075 strain, a producer of a new anthracycline antibiotic (so-075r1) and its mutant strain (ifm 0075-13-1). the structural studies showed that they are reduced anthracyline related compounds. some biosynthetic routes of these metabolites were discussed.	1994	7928699
lymphocutaneous nocardia brasiliensis infection transmitted via a cat scratch: a second case.		1994	8038327
nodular lymphadenitis caused by nocardia brasiliensis.		1994	8075295
unusually located lymphocutaneous nocardiosis caused by nocardia brasiliensis.	we report a patient with primary lymphocutaneous nocardia brasiliensis infection affecting the face and left arm. the mode of infection was via skin abrasions which occurred 2 weeks prior to the development of the skin lesions. treatment with intravenous minocycline for 4 weeks resulted in a cure. we also review 12 previously reported japanese cases of lymphocutaneous nocardiosis.	1995	7748754
concurrent chromoblastomycosis caused by fonsecaea pedrosoi and actinomycetoma caused by nocardia brasiliensis.	chromoblastomycosis and mycetoma are chronic infections caused by the repeated traumatic inoculation of organisms into the skin and subcutaneous tissue. chromoblastomycosis is caused by several dematiaceous (pigmented) fungi, occurs in tropical regions, and usually affects the lower extremities. the lesions may be nodules, plaques, or tumorous masses. mycetomas are caused by either true fungi (eumycetes) or filamentous bacteria (actinomycetes); they also occur predominantly in tropical areas and ...	1995	7829748
lymphocutaneous nocardia brasiliensis infection: a case report and review.		1995	7566789
new nocardia taxon among isolates of nocardia brasiliensis associated with invasive disease.	nocardia brasiliensis, the second most frequently isolated aerobic actinomycete in the clinical laboratory, is usually associated with localized cutaneous infections. however, 22% of 238 n. brasiliensis isolates from the united states and 12% of 66 isolates from queensland, australia, which had been collected over a 17-year period, were associated with extracutaneous and/or disseminated diseases. of the 62 invasive isolates, 37 (60%) were susceptible to ciprofloxacin and/or were susceptible to c ...	1995	7650180
brain abscess due to nocardia brasiliensis hematogenously spread from a pulmonary infection.		1995	8562775
nocardia brasiliensis: in vitro and in vivo growth response to steroid sex hormones.	as actinomycetoma is more frequent in males than in females, the possibility that hormones might modify the nocardia brasiliensis growth and the course of experimental actinomycetoma was explored. five n. brasiliensis strains were grown on sabouraud agar containing estradiol, progesterone or testosterone, in 3 different concentrations. colony diameters were measured weekly for 7 weeks. n. brasiliensis strains were also grown in sabouraud broth containing hormones. glucose concentration was measu ...	1995	8819830
a case of lymphocutaneous nocardiosis with a review of lymphocutaneous nocardiosis reported in japan.	an 82-year-old japanese male developed nodules and ulcers along the lymphatics after a fall in the garden of his house resulting in injuries to the dorsum of his left hand which lasted for 3 months. nocardia brasiliensis was isolated from a nodule, supporting a diagnosis of the lymphocutaneous type of nocardiosis. he had previously developed generalized bone metastasis from prostatic cancer, and his resulting depressed immunity might have played a part in the nocardiosis genesis. sixteen cases o ...	1996	8839240
activities of the glycylcyclines n,n-dimethylglycylamido-minocycline and n,n-dimethylglycylamido-6-demethyl-6-deoxytetracycline against nocardia spp. and tetracycline-resistant isolates of rapidly growing mycobacteria.	susceptibilities to the new semisynthetic tetracycline (tet) compounds n,n-dimethylglycylamido-minocycline (dmg-mino) and n,n-dimethylglycylamido-6-demethyl-6-deoxytetracycline (dmg-dmdot) were compared with those to doxycycline, minocycline, and tet for 198 tet-resistant (tetr) and 33 tet-susceptible (tets) clinical isolates of rapidly growing mycobacteria (rgm) including the mycobacterium fortuitum group, mycobacterium abscessus, mycobacterium chelonae, and mycobacterium mucogenicum and 68 iso ...	1996	8849243
a rapid and gentle method for isolation of genomic dna from pathogenic nocardia spp.	the lack of simple and efficient methods for extraction of dna from nocardia spp. has hampered molecular manipulation of the dna for diagnostic purposes. in the present study, a method for the rapid extraction of undegraded genomic nocardial dna was established. briefly, 14 pathogenic nocardia strains were grown at 37 degrees c for 3 to 5 days in sauton broth containing 0.05% tween 80. subsequently, the cultures were treated for 48 h with 1.2 mg of cycloserine per ml (final concentration). cells ...	1996	8877144
[mycetoma: report of 3 cases in falcón state, venezuela].	this is a report of three cases of mycetome studied in our laboratory during the last four years. we also informed about the evolution of the first case produced by pyrenochaeta romeroi nova species reported by borelli in 1959. all the patients were farmer men, 18, 42 and 54 years old, from the semiarid and subhumid zones of falcon state, located on the northwest region of venezuela. clinically, all patients presented the mycetome syndrome (subcutaneous edema, sinus tract and "granules"), with a ...	1996	8920032
nocardia brasiliensis keratitis successfully treated with therapeutic lamellar keratectomy.	a 36-year-old man sustained an indolent keratitis following a minor corneal trauma. nocardia brasiliensis was isolated from cultures of the corneal scrapings. fortified topical antibiotics, based on in vitro drug sensitivity testing, were intensively applied for weeks without clinical improvement. the patient underwent therapeutic lamellar keratectomy, which led to a rapid and complete resolution. the site of the keratectomy reepithelialized within 2 days and became a faint subepithelial haze th ...	1996	8925664
role of passive humoral immunity in experimental mycetoma by nocardia brasiliensis.		1996	8993374
nocardia pseudobrasiliensis sp. nov., a new species of nocardia which groups bacterial strains previously identified as nocardia brasiliensis and associated with invasive diseases.	we studied five strains of a new nocardia taxon recently identified among nocardia brasiliensis strains associated with invasive diseases (r. j. wallace, jr., b. a. brown, z. blacklock, r. ulrich, k. jost, j. m. brown, m. m. mcneil, g. onyi, v. a. steingrube, and j. gibson, j. clin. microbiol. 33:1528-1533, 1995) to determine their taxonomic status. several characteristics of these organisms, including the presence of chemotype iv cell walls, nocardomycolic acids, a predominant menaquinone simil ...	1996	8573505
an acute nocardia brasiliensis infection of the hand: a case report.		1996	8683074
a palmar chancre and multiple proximal erythematous nodules. nodular lymphangitis due to nocardia brasiliensis.		1996	8712850
isolation and characterisation of nocardia from clinical specimens.	sixteen isolations of nocardia of which 12 were from pulmonary infections, one from wound infection, one from mycetoma and 2 from eye infections were studied from june, 1989 to may, 1990. the importance of gram's stain findings of primary smear is being highlighted. the nocardia species were identified utilising the morphological characters including acid fastness and cultural and biochemical characters. notable among the isolates were nocardia brasiliensis, one each from mycetoma and pulmonary ...	1996	8810179
brasiliquinones a, b and c, new benz[alpha]anthraquinone antibiotics from nocardia brasiliensis. i. producing strain, isolation and biological activities of the antibiotics.	new benz[alpha]anthraquinone antibiotics (brasiliquinones a, b and c) with an ethyl group at c-3 were isolated. the producer was identified as nocardia brasiliensis. the antibiotics were active against gram-positive bacteria including mycobacterium sp., but not active against gram-negative bacteria or fungi. they were also active against multiple drug-resistant p388/adr tumor cells.	1997	9066761
monoclonal antibodies to p24 and p61 immunodominant antigens from nocardia brasiliensis.	we prepared a nocardia brasiliensis cell extract and purified two immunodominant antigens with molecular weights of 61,000 and 24,000. the isolated proteins were shown to be reasonably pure when analyzed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (8 to 18% polyacrylamide gradient) and stained with coomassie blue and silver nitrate. by using an immunoelectrotransfer blot method (western blotting), we demonstrated that these two purified proteins reacted strongly with serum fro ...	1997	9067645
a case of disseminated infection with nocardia brasiliensis in a lung transplant recipient.	infection with nocardia species is an increasingly recognized complication of solid organ transplantation. nocardia asteroides accounts for most transplant-related nocardiosis, while nocardia brasiliensis rarely causes infection in organ transplant recipients.	1997	9133485
brasilidine a, a new cytotoxic isonitrile-containing indole alkaloid from the actinomycete nocardia brasiliensis.		1997	9249977
[mastitis caused by nocardia brasiliensis in an immunocompetent patient].		1997	9376413
brasilinolide a, a new macrolide antibiotic produced by nocardia brasiliensis: producing strain, isolation and biological activity.	a new 32-membered macrolide antibiotic, brasilinolide a was isolated from the fermentation broth of nocardia sp. ifm 0406. the producer was identified as nocardia brasiliensis. the antibiotic was only active against aspergillus niger, but not active against other fungi including yeasts as well as other filamentous like fungi and bacteria. brasilinolide a exerted an immunosuppressive activity in the assay system of a mixed lymphocyte reaction (mlr).	1997	9510911
localized cutaneous nocardia brasiliensis mimicking foreign body granuloma.	nocardia brasiliensis is recognized in the united states as an infectious agent of the skin that may present as an abscess, ulcer, or granuloma with or without "sporotrichoid" spread, and rarely causes systemic disease. treatment with trimethoprim/sulfamethoxazole is usually curative. we present a patient with multiple erythematous, painful, draining nodules that developed thirty-six months after trauma and subsequent contamination with soil in bermuda. a foreign body granuloma was suspected cli ...	1998	9538960
nothramicin, a new anthracycline antibiotic from nocardia sp. mj896-43f17.	a new antibiotic designated nothramicin was isolated from the culture broth of nocardia sp. mj896-43f17 which was closely related to nocardia brasiliensis. it was isolated by the diaion hp-20 column chromatography, butyl acetate extraction and purified by hplc. it inhibited the growth of mycobacteria at the concentration of 1.56 approximately 25 microg/ml. nothramicin was revealed to be a new member of anthracycline antibiotics by the various spectroscopies.	1998	9544933
antimicrobial susceptibilities of strains of nocardia brasiliensis isolated from soil of tucumán.	the activity of antimicrobial agents against soil isolates of n. brasiliensis was studied by determination of the minimum inhibitory concentrations (mics) and disk diffusion technique, according to the national committee for clinical laboratory standards (nccls). the objectives were: (a) to study the patterns of sensitivity among regional strains of n. brasiliensis isolated from natural sources (soil) of different zones of tucumán province; (b) to correlate these results with those previously ob ...	1998	9755502
rhodococcus equi and nocardia brasiliensis infection of the brain and liver in a patient with acute nonlymphoblastic leukemia.	resolution of neutropenia is usually followed by resolution of fever in patients with febrile neutropenia. however, in some cases fever continues even when the patient is no longer neutropenic. described here is a case of acute myeloblastic leukemia complicated by brain abscess, pulmonary nodules, and hepatic lesions. the patient's fever had continued after the neutropenia resolved; brain and hepatic cultures grew rhodococcus equi and nocardia brasiliensis. although rhodococcus infections occur ...	1998	9865991
brasilicardin a. a novel tricyclic metabolite with potent immunosuppressive activity from actinomycete nocardiabrasiliensis.	a novel tricyclic metabolite, brasilicardin a (1), with potent immunosuppressive activity has been isolated from the cultured broth of the actinomycete nocardia brasiliensis ifm 0406, and the structure including absolute stereochemistry was established on the basis of spectroscopic data, chemical means, and x-ray analysis. brasilicardin a (1) is the first tricyclic compound consisting of an anti/syn/anti-perhydrophenanthrene skeleton with a rhamnose, an n-acetylglucosamine, and an amino acid moi ...	1998	11672311
disseminated nocardia brasiliensis infection in an immunocompromised patient.		1998	10761018
brasilicardin a, a new terpenoid antibiotic from pathogenic nocardia brasiliensis: fermentation, isolation and biological activity.	a novel tricyclic diterpenoid antibiotic, brasilicardin a, was isolated from the culture broth of nocardia brasiliensis ifm 0406. the antibiotic exhibited immunosuppressive activity in a mouse mixed lymphocyte reaction (mlr) assay system and its ic50 value was 0.057 microg/ml. although the inhibitory activity of cyclosporin a (cya) against il-2 production was confirmed in the mlr assay system, brasilicardin a did not have the activity. the results of in vitro toxicity testing of brasilicardin a ...	1999	10092191
immune response to nocardia brasiliensis antigens in an experimental model of actinomycetoma in balb/c mice.	nine- to twelve-week-old balb/c mice were injected in footpads with 10(7) cfu of a nocardia brasiliensis cell suspension. typical actinomycetoma lesions, characterized by severe local inflammation with abscess and fistula formation, were fully established by day 28 after infection. these changes presented for 90 days, and then tissue repair with scar formation slowly appeared, with complete healing after 150 days of infection. some animals developed bone destruction in the affected area. histopa ...	1999	10225905
distribution of a nocardia brasiliensis catalase gene fragment in members of the genera nocardia, gordona, and rhodococcus.	an immunodominant protein from nocardia brasiliensis, p61, was subjected to amino-terminal and internal sequence analysis. three sequences of 22, 17, and 38 residues, respectively, were obtained and compared with the protein database from genbank by using the blast system. the sequences showed homology to some eukaryotic catalases and to a bromoperoxidase-catalase from streptomyces violaceus. its identity as a catalase was confirmed by analysis of its enzymatic activity on h2o2 and by a double-s ...	1999	10325357
cutaneous nocardiosis caused by nocardia brasiliensis after an insect bite.	we report the case of a primary lymphocutaneous nocardiosis occurring on the right calf of a healthy 56-year-old man after an insect bite. analysis of the purulent exudate obtained from the nodule revealed nocardia brasiliensis. the initial therapy with trimethoprim-sulfamethoxazole had to be stopped due to a drug eruption. however, with minocycline treatment the patient recovered within 5 weeks. superficial (sporotrichoid) infections and a history of outdoor injury should be considered suspicio ...	1999	10325464
production of erythromycin e by pathogenic nocardia brasiliensis.		1999	10344579
[combined cefotaxime and amikacin for immunomodulation in the treatment of actinomycetoma resistant to conventional treatment].	actinomycetoma is a chronic disease that affects subcutaneous tissue. we present a case of a patient with abdominal actinomycetoma caused by nocardia brasiliensis resistant to different treatments over several years, who also presented phagocyte immunodeficiency. he received two cycles (23 day cycles) of cefotaxime, 1 g every 8-h, and amikacin, 500 mg every 12 hours. immunomodulation was carried out with levamisole 300 mg per week, during 4 weeks and bacterial antigen (at a concentration of 600, ...	1999	10596493
humoral response in a patient with cutaneous nocardiosis.	the clinical appearance of infection due to nocardia spp. varies widely. the low sensitivity of direct microscopy and the slow growth of the organism challenge the laboratory diagnosis. we present the case of a skin abscess in an immunocompetent man caused by nocardia brasiliensis. diagnosis was made by cultivation and 16s rrna sequencing. using indirect immunofluorescence and western blot, a strong antibody response to the n. brasiliensis isolate could be demonstrated. serological tests might t ...	2000	10681624
a new antifungal macrolide component, brasilinolide b, produced by nocardia brasiliensis.		2000	10724012
antitumor activity of brasilicardin a, a novel terpenoid antibiotic from nocardia brasiliensis.		2000	10724013
two cases of mycetoma due to nocardia brasiliensis in central australia.	comparatively few cases of mycetoma ("madura foot") have been reported in australia, and only one caused by nocardia brasiliensis. we report two aboriginal women from remote communities in central australia who presented with longstanding mycetomas caused by this organism. difficulties in diagnosis substantially delayed commencement of effective treatment, illustrating the need to consider this condition in chronic suppurative skin infection.	2000	10772588
head actinomycetoma with a double aetiology, caused by nocardia brasiliensis and n. asteroides.	we describe a 64-year-old man with an actinomycetoma of the frontal region of the head caused simultaneously by two aetiological agents, nocardia brasiliensis and n. asteroides. this case is presented due to the unusual body site affected and, above all, because two aetiological agents were isolated.	2000	10886160
nocardia brasiliensis: from microbe to human and experimental infections.	nocardia brasiliensis is a gram-positive bacterium that lives as a saprophyte in soil. in this article the physical properties, chemical composition and taxonomic position of this species is reviewed. human infections and an experimental model of actinomycetoma in balb/c mice as well as the host-immune response is described.	2000	11018454
lymphocutaneous nocardia brasiliensis infection: a pediatric case cured with amoxicilin/clavulanate.		2000	11055613
[nodular lymphangitis caused by nocardia asteroides].	nocardia species are typically found in soil and decaying organic matter. the lymphocutaneous type or nodular lymphangitis is unusual. this is acquired in most of cases by a traumatic inoculation through the skin of a person who is immunocompetent, and is generally caused by nocardia brasiliensis.	2000	11153201
sporotrichoid lymphocutaneous infections: etiology, diagnosis and therapy.	sporotrichoid lymphocutaneous infection is an uncommon syndrome that is often misdiagnosed and improperly treated. of the several hundred cases seen each year in the united states, the majority are caused by sporothrix schenckii, nocardia brasiliensis, mycobacterium marinum or leishmania brasiliensis. the "sporotrichoid" disease begins at a site of distal inoculation and leads to the development of nodular lymphangitis. systemic symptoms are characteristically absent. by recognizing the distinct ...	2001	11201697