Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a ste12 homolog is required for mating but dispensable for filamentation in candida lusitaniae. | candida lusitaniae is a dimorphic yeast that is emerging as an opportunistic fungal pathogen. in contrast to candida albicans, which is diploid and asexual, c. lusitaniae has been reported to have a sexual cycle. we have employed genetic approaches to demonstrate that c. lusitaniae is haploid and has a sexual cycle involving mating between mata and matalpha cells under nutrient deprivation conditions. by degenerate pcr, we identified a c. lusitaniae homolog (cls12) of the ste12 transcription fac ... | 2000 | 10790381 |
| comparative evaluation of a commercial system for identification of candida lusitaniae. | the ability of the api candida system (biomérieux, france) to identify candida lusitaniae isolates was evaluated in comparison to the auxacolor and id 32c systems using 52 clinical isolates previously identified on the basis of their morphology and their biochemical reactions in the auxacolor and id 32c systems. the api candida system failed to definitively identify most of the strains tested within 24 h. no beta-maltosidase activity was detected in 28 strains, and supplementary tests were requi ... | 2000 | 10898145 |
| flow cytometry antifungal susceptibility testing of pathogenic yeasts other than candida albicans and comparison with the nccls broth microdilution test. | candida species other than candida albicans frequently cause nosocomial infections in immunocompromised patients. some of these pathogens have either variable susceptibility patterns or intrinsic resistance against common azoles. the availability of a rapid and reproducible susceptibility-testing method is likely to help in the selection of an appropriate regimen for therapy. a flow cytometry (fc) method was used in the present study for susceptibility testing of candida glabrata, candida guilli ... | 2000 | 10991856 |
| nosocomial candidemia in non-neutropenic patients at an italian tertiary care hospital. | in a retrospective study conducted in an italian tertiary care hospital, the incidence of nosocomial candidemia was evaluated together with causative pathogens, treatment, and risk factors for death. over a 6-year period (1992-1997), a total of 189 episodes of candidemia occurred in 189 patients (mean age 58+/-19 years), accounting for an average incidence of 1.14 episodes per 10,000 patient-days per year. the most common reasons for hospitalization were solid neoplasia (21%), trauma (17%), abdo ... | 2000 | 11014622 |
| identification of rare candida species and other yeasts by polymerase chain reaction and slot blot hybridization. | invasive candidiasis has become a major cause of morbidity and mortality in immunocompromised hosts. here we describe a fast and reliable dna extraction and pcr amplification method in combination with a slot blot hybridization assay. a genus-specific probe was designed that allowed to detect dna from a broad range of candida species and 3 other yeasts. in addition, species-specific oligonucleotides for emerging candida and other yeast species allowed to identify dna extracted from candida lusit ... | 2000 | 11146245 |
| influence of glucose supplementation and inoculum size on growth kinetics and antifungal susceptibility testing of candida spp. | the influences of inoculum size and glucose supplementation on the growth kinetics of 60 candida spp. clinical isolates (candida albicans, candida tropicalis, candida parapsilosis, candida glabrata, candida krusei, and candida lusitaniae [10 isolates each]) are assessed. the combined influence of growth and reading method (visual or spectrophotometric) on the determination of the mics of amphotericin b, flucytosine, fluconazole, itraconazole, ketoconazole, and voriconazole is also analyzed, and ... | 2001 | 11158101 |
| candida lusitaniae: a cause of breakthrough fungemia in cancer patients. | candida lusitaniae is an infrequent cause of fungemia. we identified 12 cases of c. lusitaniae fungemia that occurred at the university of texas m. d. anderson cancer center from 1988 to 1999. the mean age of patients was 48 years (range 20--70 years). eight patients had hematologic malignancy or had received a bone marrow transplant, and 4 had a solid tumor. most patients (75%) were neutropenic (<10(3)/mm(3)). treatment with amphotericin b alone failed for 3 of 6 patients, irrespective of neutr ... | 2001 | 11170906 |
| [contamination of dairy products by yeasts-like fungi]. | evaluation of the degree of contamination of diary products with potentially pathogenic fungal strains. materials for the study were: yoghurt, cottage cheese, homogenised cottage cheese, cream and buttermilk. differentiation of species was based on their morphological features and biochemical properties (api 20 c biomérieux). among 300 studied diary products we have isolated 100 (33.0 +/- 2.72%) strains of yeast-like fungi. diary products were contamined with 19 species of fungi from types: cand ... | 2001 | 16894745 |
| improved detection of amphotericin b-resistant isolates of candida lusitaniae by etest. | both intrinsic and acquired resistance to amphotericin b have been documented for candida lusitaniae. amphotericin b remains the drug of choice for many critical fungal infections, and the detection of resistance is essential to monitor treatment effectively. the limitations of the national committee for clinical laboratory standards (nccls) reference methodology for detection of amphotericin b resistance are well documented, and several alternative methods have been proposed. etest assays with ... | 2001 | 11136795 |
| fluconazole and voriconazole multidisk testing of candida species for disk test calibration and mic estimation. | fluconazole and voriconazole mics were determined for 114 clinical candida isolates, including isolates of candida albicans, candida glabrata, candida krusei, candida lusitaniae, candida parapsilosis, and candida tropicalis. all strains were susceptible to voriconazole, and most strains were also susceptible to fluconazole, with the exception of c. glabrata and c. krusei, the latter being fully fluconazole resistant. single-strain regression analysis (sra) was applied to 54 strains, including am ... | 2001 | 11283066 |
| secreted aspartic proteases of candida albicans, candida tropicalis, candida parapsilosis and candida lusitaniae. inhibition with peptidomimetic inhibitors. | the frequency of candida infections has increased in recent years and it has been accompanied by a significant rise in morbidity and mortality. the secretion of aspartic proteases by candida spp. was demonstrated to be one of the virulence determinants. candida albicans is classified as the major human pathogen in the genus candida. however, other species of this genus have been found to cause an increasing number of candidiases. we isolated secreted aspartic proteases (saps) of c. albicans (sap ... | 2001 | 11322888 |
| azasordarins: susceptibility of fluconazole-susceptible and fluconazole-resistant clinical isolates of candida spp. to gw 471558. | the in vitro activity of the azasordarin gw 471558 was compared with those of amphotericin b, flucytosine, itraconazole, and ketoconazole against 177 clinical isolates of candida spp. gw 471558 showed potent activity against candida albicans, candida glabrata, and candida tropicalis, even against isolates with decreased susceptibility to azoles. candida krusei, candida parapsilosis, candida lusitaniae, and candida guilliermondii are resistant to gw 471558 in vitro (mics, >128 microg/ml). | 2001 | 11353650 |
| systemic antifungal agents. | anti-fungal agents are classified under two major headings, systematic and topical agents. only systematic anti-fungal agents will be discussed in this chapter. since the discovery in 1955, amphotericin b has been the cornerstone of anti-fungal treatment. it is active against most species of fungi. however, candida lusitaniae, pseudallescheria boydii, and fusarium spp have primary resistance to amphotericin b. recently, new liposomal preparations of amphotericin b have been developed. they are l ... | 2001 | 11519290 |
| decaying wood in tree trunk hollows as a natural substrate for cryptococcus neoformans and other yeast-like fungi of clinical interest. | the occurrence of cryptococcus neoformans var. neoformans and other yeast-like fungi of clinical interest in decaying wood inside tree trunk hollows, bark and other plant materials is reported. the var. neoformans was isolated from 3 of 45 (6.6%) wood and one of 390 eucalyptus bark samples. two of the positive wood samples came from a tree trunk hollow of butea monosperma (family: papilionaceae) growing in roshan ara garden, old delhi whereas the third was from a trunk hollow of tamarindus indic ... | 2001 | 11554580 |
| alternative identification test relying upon sexual reproductive abilities of candida lusitaniae strains isolated from hospitalized patients. | the in vitro mating ability of candida lusitaniae (teleomorph clavispora lusitaniae) clinical isolates has been investigated. studying the effects of culture conditions, we showed that ammonium ion depletion in the medium is a major trigger of the sexual cycle. moreover, a solid support is required for mating, suggesting a role for adhesion factors in addition to the mating type gene recognition function. monitoring of mating and meiosis efficiency with auxotrophic strains showed great variation ... | 2001 | 11682506 |
| evaluation of etest method for determining caspofungin (mk-0991) susceptibilities of 726 clinical isolates of candida species. | the performance of the etest for testing the susceptibilities to caspofungin (mk-0991) of 726 isolates of candida spp. was assessed against the national committee for clinical laboratory standards (nccls) microdilution broth method. the nccls method employed rpmi 1640 broth medium, and mics were read after incubation for 48 h at 35 degrees c. mics were determined by etest for all 726 isolates with rpmi agar containing 2% glucose (rpg) and were read after incubation for 48 h at 35 degrees c. the ... | 2001 | 11724849 |
| candida lusitaniae catheter-related sepsis. | to present a case describing candida lusitaniae candidemia in an immunocompetent patient successfully treated with fluconazole antifungal therapy. time-kill studies of the c. lusitaniae isolate using amphotericin b, and an extensive review of the literature are also presented. | 2001 | 11793623 |
| candida lusitaniae as an unusual cause of recurrent vaginitis and its successful treatment with intravaginal boric acid. | increasing use of short-course antifungal therapies in patients with recurrent vulvovaginitis may enable the emergence of less-common, more resistant yeast strains as vaginal pathogens. we report the case of a patient with chronically symptomatic and repeatedly treated vaginal candidiasis whose infection was attributable to candida lusitaniae, a previously unreported cause of candidal vaginitis. | 2001 | 11916183 |
| [evaluation of a new chromogenic medium (candida id) for the isolation and presumptive identification of candida albicans and other medically important yeasts]. | candidiasis is a frequent human infection caused mainly by candida albicans. however, other species are emerging as important pathogens, as candida glabrata, candida parapsilosis, candida tropicalis, candida krusei or candida guilliermondii. rapid identification of clinical isolates could facilitate diagnosis and treatment. candida id (biomerieux, spain) is a new medium for the isolation and presumptive identification of yeasts: c. albicans grows as blue colonies, and c. tropicalis, c. guillierm ... | 2001 | 15482010 |
| yeast species associated with orange juice: evaluation of different identification methods. | five different methods were used to identify yeast isolates from a variety of citrus juice sources. a total of 99 strains, including reference strains, were identified using a partial sequence of the 26s rrna gene, restriction pattern analysis of the internal transcribed spacer region (5.8s-its), classical methodology, the rapid yeast plus system, and api 20c aux. twenty-three different species were identified representing 11 different genera. distribution of the species was considerably differe ... | 2002 | 11916718 |
| change in colony morphology of candida lusitaniae in association with development of amphotericin b resistance. | it is not uncommon to see amphotericin b treatment failure in patients with systemic infection caused by candida lusitaniae. we report a patient with stage iv ovarian carcinoma and c. lusitaniae sepsis whose treatment with amphotericin b failed. the initial blood isolate was susceptible to amphotericin b in vitro; however, the mic for a blood isolate recovered 7 weeks after treatment began showed a fourfold increase. direct subculture of two positive blood samples obtained within a week of the p ... | 2002 | 11959563 |
| case report. candida lusitaniae peritonitis in a patient on continuous ambulatory peritoneal dialysis. | we report a case of candida lusitania peritonitis in continuous ambulatory peritoneal dialysis. since fluconazole therapy was not successful in this patient, the peritoneal catheter was removed and antifungal therapy continued, and the patient was then converted to haemodialysis. this treatment protocol was successful. we suggest that early peritoneal catheter removal should be considered in such cases. | 2002 | 12000515 |
| fatal disseminated candida lusitaniae infection in an infant with chronic granulomatous disease. | a 3-month-old boy born to a mother carrying an x-linked form of chronic granulomatous disease presented with persistent fever and hepatosplenomegaly. the diagnosis was confirmed as a gp91phox defect by genetic analysis, and the patient was managed with broad spectrum antibacterial agents, gamma-interferon and later amphotericin b. a liver biopsy revealed granulomata with budding yeast forms, and cultures of blood and urine grew candida lusitaniae. the patient died 26 days after admission. | 2002 | 12005097 |
| two cases of fungemia due to candida lusitaniae and a literature review. | reported here are two cases of candidemia caused by candida lusitaniae that occurred in two immunocompromised patients at hospital universitario "la fe" in valencia, spain. case 1 involved a low-birth-weight premature infant with congenital nephrotic syndrome who was successfully treated with amphotericin b, and case 2 involved a 50-year old woman with a high-grade malignancy lymphoma who succumbed to the infection. antifungal susceptibility testing of the candida lusitaniae isolates recovered f ... | 2002 | 12072941 |
| new chromogenic agar medium for the identification of candida spp. | a new chromogenic agar medium (candida diagnostic agar [cda]) for differentiation of candida spp. is described. this medium is based on sabouraud dextrose agar (oxoid cm41) and contains (per liter) 40.0 g of glucose, 10.0 g of mycological peptone, and 15.0 g of agar along with a novel chromogenic glucosaminidase substrate, ammonium 4-(2-[4-(2-acetamido-2-deoxy-beta-d-glucopyranosyloxy)-3-methoxyphenyl]-vinyl)-1-(propan-3-yl-oate)-quinolium bromide (0.32 g liter(-1)). the glucosaminidase substrat ... | 2002 | 12089051 |
| in vivo pathogenicity of eight medically relevant candida species in an animal model. | the relative pathogenicity of eight medically important candida species was investigated in a mouse model. | 2002 | 12382088 |
| identification and antifungal susceptibility of candida isolated from intensive care unit patients. | candida was isolated in 205 of 1060 clinical specimens (19.33%) in our laboratary sent from the intensive care unit for mycological investigation between january 98-december 99. all isolated strains were identified to species level using the api candida system (bio-meieux, france) as follows; candida albicans (n:115, 56.09%), candida tropicalis (n:23, 11.21%), candida parapsilosis (n:21, 10.24%), candida glabrata (n:12, 5.83%). candida kefyr (n:9, 4.39%), candida lusitaniae (n:7, 3.41%), candida ... | 2002 | 12437230 |
| sterol and fatty acid composition of candida lusitaniae clinical isolates. | the sterol and fatty acid compositions of four amphotericin b-resistant and of two amphotericin b-susceptible candida lusitaniae clinical isolates were determined. a flow cytofluorometric susceptibility test (fcst) with a membrane potential-sensitive cationic dye was used as a complement to the conventional method for selecting the isolates. compared to susceptible isolates, resistant ones showed a greatly reduced ergosterol content and changes in sterol composition, consistent with a defect in ... | 2002 | 11796371 |
| rates and extents of antifungal activities of amphotericin b, flucytosine, fluconazole, and voriconazole against candida lusitaniae determined by microdilution, etest, and time-kill methods. | amphotericin b, flucytosine, fluconazole, and voriconazole alone and in combination were evaluated against isolates of candida lusitaniae. mics were determined by broth microdilution and etest, and time-kill studies were conducted. amphotericin b resulted in fungicidal activity against most isolates, whereas fluconazole, voriconazole, and flucytosine produced primarily fungistatic activities. the addition of flucytosine to amphotericin b resulted in a faster rate and greater extent of kill for i ... | 2002 | 11796383 |
| amphotericin b: spectrum and resistance. | amphotericin b is a polyene macrolide antibiotic derived from the actinomycete streptomyces nodosus. of the 200 known polyene agents, amphotericin b is the only one with toxicities that are sufficiently limited to permit intravenous administration. all polyenes have a common mechanism of action in that they preferentially bind to ergosterol, the primary sterol in the fungal cell membrane. the consequence of this binding includes disruption of the osmotic integrity of the membrane, with leakage o ... | 2002 | 11801575 |
| candida lusitaniae infections in the era of fluconazole availability. | amphotericin b resistance among isolates of candida lusitaniae has distinguished it among candida species. because no comprehensive review has been published recently, we provide a case report and a literature review of c. lusitaniae infection to update and better characterize the illness in the era of azole availability and standardized methodologies for antifungal susceptibility testing. c. lusitaniae infection in the 55 cases surveyed in this review occurred in relatively young patients (medi ... | 2003 | 12522762 |
| comparison of three methods for in vitro susceptibility testing of candida species with flucytosine. | optimal methods for susceptibility testing of candida spp. with flucytosine have not been determined. breakpoints were recommended in 1984, but never validated. in this study, we compared the 1984 recommended macrodilution broth method (using an 80% endpoint) with a modification of the more recent nccls-recommended microdilution broth method with three endpoints-spectrophotometric 50% and 80% and a no growth endpoint determined by eye. nccls and british society for medical mycology (bsmm) breakp ... | 2003 | 12562694 |
| proficiency testing program for clinical laboratories performing antifungal susceptibility testing of pathogenic yeast species. | antifungal susceptibility testing is expected to facilitate the selection of adequate therapy for fungal infections. the general availability of antifungal susceptibility testing in clinical laboratories is low, even though a number of standard methods are now available. the objective of the present study was to develop and evaluate a proficiency testing program (ptp) for the antifungal susceptibility testing of pathogenic yeasts in laboratories licensed by the new york state department of healt ... | 2003 | 12624043 |
| flucytosine-fluconazole cross-resistance in purine-cytosine permease-deficient candida lusitaniae clinical isolates: indirect evidence of a fluconazole uptake transporter. | an unusual interaction between flucytosine and fluconazole was observed when a collection of 60 candida lusitaniae clinical isolates was screened for cross-resistance. among eight isolates resistant to flucytosine (mic >/= 128 micro g/ml) and susceptible to fluconazole (0.5 < mic < 2 micro g/ml), four became flucytosine-fluconazole cross resistant when both antifungals were used simultaneously. fluconazole resistance occurred only in the presence of high flucytosine concentrations, and the highe ... | 2003 | 12654658 |
| development and evaluation of the nuclisens basic kit nasba for the detection of rna from candida species frequently resistant to antifungal drugs. | we describe a nucleic acid sequence based amplification (nasba) protocol to detect 6 different candida species (candida krusei, candida glabrata, candida inconspicua, candida dubliniensis, candida norvegensis, candida lusitaniae) and compare it to a pcr assay. nasba showed a sensitivity of 1 colony forming unit and detected rna from all 6 candida species within 1 working day. all 5 patients with documented candidiasis showed identical results by both methods. this assay offers a sensitive, speci ... | 2003 | 12663165 |
| identification of yeast species from orange fruit and juice by rflp and sequence analysis of the 5.8s rrna gene and the two internal transcribed spacers. | yeast isolates from orange fruit and juice in a spontaneous fermentation were identified and classified by two molecular techniques. the first was analysis of the restriction pattern generated from the polymerase chain reaction (pcr)-amplified 5.8s rrna gene and the two internal transcribed spacers (its) using specific primers. the second technique was sequence analysis of the its regions using the same two primers. nine different restriction profiles were obtained from the size of the pcr produ ... | 2003 | 12702240 |
| existence of novel beta-1,2 linkage-containing side chain in the mannan of candida lusitaniae, antigenically related to candida albicans serotype a. | the antigenicity of candida lusitaniae cells was found to be the same as that of candida albicans serotype a cells, i.e. both cell wall mannans react with factors 1, 4, 5, and 6 sera of candida check. however, the structure of the mannan of c. lusitaniae was significantly different from that of c. albicans serotype a, and we found novel beta-1,2 linkages among the side-chain oligosaccharides, manbeta1-->2manbeta1--> 2manalpha1-->2manalpha1-->2man (lm5), and manbeta1-->2man-beta1-->2manbeta1-->2m ... | 2003 | 12787022 |
| genetic relatedness of candida strains isolated from women with vaginal candidiasis in malaysia. | the aims of this study were to compare the genetic relatedness of: (i) sequential and single isolates of candida strains from women with recurrent vaginal candidiasis (rvc); and (ii) candida strains from women who had only one episode of infection within a 1-year period. in total, 87 isolates from 71 patients were cultured, speciated and genotyped by random amplification of polymorphic dna (rapd) analysis. patients were categorized into three groups, namely those with: (i) a history of rvc from ... | 2003 | 12867559 |
| a monoclonal antibody directed against a candida albicans cell wall mannoprotein exerts three anti-c. albicans activities. | antibodies are believed to play a role in the protection against candida albicans infections by a number of mechanisms, including the inhibition of adhesion or germ tube formation, opsonization, neutralization of virulence-related enzymes, and direct candidacidal activity. although some of these biological activities have been demonstrated individually in monoclonal antibodies (mabs), it is not clear if all these anti-c. albicans activities can be displayed by a single antibody. in this report, ... | 2003 | 12933874 |
| disruption of ergosterol biosynthesis confers resistance to amphotericin b in candida lusitaniae. | candida lusitaniae is an emerging human pathogen that, unlike other fungal pathogens, frequently develops resistance to the commonly used antifungal agent amphotericin b. amphotericin b is a member of the polyene class of antifungal drugs, which impair fungal cell membrane integrity. here we analyzed mechanisms contributing to amphotericin b resistance in c. lusitaniae. sensitivity to polyenes in the related fungi saccharomyces cerevisiae and candida albicans requires the ergosterol biosynthetic ... | 2003 | 12936965 |
| colony morphology switching of candida lusitaniae and acquisition of multidrug resistance during treatment of a renal infection in a newborn: case report and review of the literature. | candida lusitaniae is an emerging opportunistic pathogen which exhibits an unusual antifungal susceptibility pattern. we describe a case of fatal renal infection due to c. lusitaniae in a very low birth weight neonate who was treated with short courses of fluconazole given alternately with amphotericin b. a colony morphology switching was detected on the standard primary culture medium by changes in colony size. switching was shown to affect deeply the susceptibility to amphotericin b. afterward ... | 2003 | 12967746 |
| clavispora (candida) lusitaniae susceptibility profiles and genetic diversity in three tertiary hospitals (1998-2001). | 2003 | 14522112 | |
| rapid identification of yeasts commonly found in positive blood cultures by amplification of the internal transcribed spacer regions 1 and 2. | a multiplex pcr method using one universal and eight species-specific primers was developed to rapidly identify eight yeast species found in positive blood cultures. the species-specific primers were designed from the internal transcribed spacer regions 1 and 2 of the rrna gene, whereas the universal primer was located at the 26s rrna gene. the eight species were candida albicans, candida glabrata, candida guilliermondii, candida krusei, candida lusitaniae, candida parapsilosis, candida tropical ... | 2003 | 14557922 |
| rapid and unequivocal differentiation of candida dubliniensis from other candida species using species-specific dna probes: comparison with phenotypic identification methods. | candida dubliniensis is a recently described opportunistic pathogen which shares many phenotypic characteristics with candida albicans but which has been reported to rapidly acquire resistance to azole antifungal drugs. therefore, differentiation of c. dubliniensis from c. albicans becomes important to better understand the clinical significance and epidemiologic role of c. dubliniensis in candidiasis. we compared phenotypic methods for the differentiation of c. dubliniensis from c. albicans (i. ... | 2003 | 14622344 |
| the d1/d2 domain of the large-subunit rdna of the yeast species clavispora lusitaniae is unusually polymorphic. | ten different versions of the d1/d2 divergent domain of the large-subunit ribosomal dna were identified among interbreeding members of the yeast species clavispora lusitaniae. one major polymorphism, located in a 90-bp structural motif of the d2 domain, exists in two versions that differ by 32 base substitutions. three other polymorphisms consist of a two-base substitution, a two-base deletion, and a single-base deletion, respectively. the polymorphisms are independent of one another and of the ... | 2003 | 14654429 |
| development of an integrative transformation system for the opportunistic pathogenic yeast candida lusitaniae using ura3 as a selection marker. | the nucleotide sequence of the ura3 gene encoding orotidine-5'-phosphate decarboxylase (omp dcase) of the human opportunistic pathogen yeast candida lusitaniae was determined by degenerate pcr and chromosome walking. deduced amino acid sequence showed strong homologies (59-85% identity) with omp dcases of different saccharomycetales and allowed identification of the known conserved domains. very close upstream from the ura3 gene, the 3'-end of a gene encoding a gea2-like protein was identified. ... | 2004 | 14755635 |
| susceptibility of clinical isolates of candida lusitaniae to five systemic antifungal agents. | the aim of the present study was to expand the mic database for candida lusitaniae in order to further determine its antifungal susceptibility pattern. | 2004 | 14963064 |
| delineation of clavispora lusitaniae clinical isolates by polymerase chain reaction-single strand conformation polymorphism analysis of the itsi region: a retrospective study comparing five typing methods. | strain delineation of the emerging opportunistic pathogen clavispora lusitaniae was studied using 12 strains, including two strains of known opposite mating type, cbs 6936 (h+) and cbs 5094 (h-), and 10 strains isolated between 1998 and 2001 from immunocompromized patients. this retrospective study assessed the occurrence of c. lusitaniae subtypes within and among hospitals, and in outpatients who were regularly screened for fungal infections in the course of radio-chemotherapy. strain typing wa ... | 2004 | 14982111 |
| significance of amplified fragment length polymorphism in identification and epidemiological examination of candida species colonization in children undergoing allogeneic stem cell transplantation. | candida albicans and non-c. albicans candida species are increasingly being isolated from patients in high-risk categories, most notably, those who have undergone stem cell transplantation (sct). identification of the presence of non-c. albicans candida species early in the course of the transplant procedure is important, as these species exhibit different sensitivities to the available antifungal treatments and cause mortality at rates that vary from those for c. albicans. amplified fragment le ... | 2004 | 15071024 |
| the predictors of outcome in immunocompetent patients with hematogenous candidiasis. | clinical parameters that predict outcome in non-immunosuppressed candidemic patients are not fully understood. | 2004 | 15109594 |
| in vitro pharmacodynamic characteristics of amphotericin b, caspofungin, fluconazole, and voriconazole against bloodstream isolates of infrequent candida species from patients with hematologic malignancies. | time-kill and postantifungal effect (pafe) of amphotericin b, caspofungin, fluconazole, and voriconazole were determined against clinical isolates of candida guilliermondii, candida kefyr, and candida lusitaniae. azoles displayed fungistatic activity and no measurable pafe, regardless of the concentration tested. amphotericin b and caspofungin demonstrated concentration-dependent fungicidal activity, although amphotericin b only produced a significant dose-dependent pafe against all isolates tes ... | 2004 | 15504881 |
| initial results from a longitudinal international surveillance programme for anidulafungin (2003). | this longitudinal study evaluated the in vitro activity of anidulafungin against 880 clinical yeast isolates and 68 mould isolates from 64 medical centres in north america, latin america and europe. | 2004 | 15564286 |
| [performance of the candida mannan antigen detection in patients with fungemia]. | for several years, the platelia candida mannan antigen enzyme immunoassay (candida eia) has been commercially available as a diagnostic test for invasive candidosis. we evaluated the candida eia with patients with proven fungemia caused by yeasts from which at least one serum sample was available. fifty-nine patients with 121 serum samples were included in the study. sixty-one different yeast strains were isolated from positive blood-cultures. the candida eia was positive (n = 35) or borderline ... | 2004 | 15667360 |
| candida lusitaniae peritonitis in a chronic ambulatory peritoneal dialysis patient. | this is a case report of a 69 year-old african american female with end-stage renal disease secondary to hypertensive nephrosclerosis on chronic ambulatory peritoneal dialysis (capd) for five years. her past was uneventful. she presented with symptoms and signs of acute peritonitis related to capd. there was initial response to antibiotics and culture was negative. the condition of the patient deteriorated despite the antibiotic. the peritoneal catheter was removed after five days of starting th ... | 2004 | 17642771 |
| antifungal susceptibilities of clinical isolates of candida species, cryptococcus neoformans, and aspergillus species from taiwan: surveillance of multicenter antimicrobial resistance in taiwan program data from 2003. | the susceptibilities of nonduplicate isolates to six antifungal agents were determined for 391 blood isolates of seven candida species, 70 clinical isolates (from blood or cerebrospinal fluid) of cryptococcus neoformans, and 96 clinical isolates of four aspergillus species, which were collected in seven different hospitals in taiwan (as part of the 2003 program of the study group surveillance of multicenter antimicrobial resistance in taiwan). all isolates of candida species other than c. glabra ... | 2005 | 15673726 |
| differentiation between atypical isolates of candida lusitaniae and candida pulcherrima by determination of mating type. | we report on five clinical isolates routinely identified as candida lusitaniae that the id 32c system was unable to discriminate from the closely related species candida pulcherrima. when additional tests did not allow accurate identification, the less usual mating type test identified all of them as clavispora lusitaniae. mating type testing appears to be a valuable tool for assessing the true incidence of this emerging non-albicans candida species. | 2005 | 15750124 |
| chromagar candida medium for direct susceptibility testing of yeast from blood cultures. | an evaluation was performed on 95 blood cultures positive for candida spp. to determine the correlation of direct susceptibility testing of fluconazole versus both standardized disk diffusion and mic methods. for direct testing, an aliquot taken from bd bactec plus and/or bd bactec lytic/10 bottles (becton dickinson [bd], sparks, md) positive by gram stain for yeast was subcultured to chromagar candida (bd), and a 25-microg fluconazole disk (bd) was placed on the plate. the area of growth inhibi ... | 2005 | 15814992 |
| antifungal susceptibilities of candida species causing vulvovaginitis and epidemiology of recurrent cases. | there are limited data regarding the antifungal susceptibility of yeast causing vulvovaginal candidiasis, since cultures are rarely performed. susceptibility testing was performed on vaginal yeast isolates collected from january 1998 to march 2001 from 429 patients with suspected vulvovaginal candidiasis. the charts of 84 patients with multiple positive cultures were reviewed. the 593 yeast isolates were candida albicans (n = 420), candida glabrata (n = 112), candida parapsilosis (n = 30), candi ... | 2005 | 15872235 |
| antifungal susceptibility of candida spp. isolated from pediatric patients: a survey of 4 children's hospitals. | candida species are the most common cause of fungal infections in hospitalized patients. recent studies have reported a relative reduction in the rates of infection caused by candida albicans and a shift toward non-albicans candida spp. data on the distribution and susceptibility of candida spp. from children's hospitals are limited. clinical isolates of candida were collected from 4 us children's hospitals in 2003. broth dilution mics for amphotericin b, fluconazole, voriconazole, caspofungin, ... | 2005 | 15922535 |
| inactivation of the fcy2 gene encoding purine-cytosine permease promotes cross-resistance to flucytosine and fluconazole in candida lusitaniae. | in a previous work, we described the possible relationship between a defect of purine-cytosine permease and the acquisition of a cross-resistance to the antifungal combination flucytosine (5fc) and fluconazole (flc) in candida lusitaniae (t. noël, f. françois, p. paumard, c. chastin, d. brethes, and j. villard, antimicrob. agents chemother. 47:1275-1284, 2003). using degenerate pcr and chromosome walking, we cloned two fcy2-like genes in c. lusitaniae. northern blot analysis revealed that only o ... | 2005 | 16048910 |
| [fungal endocarditis found at onset of lower limb acute aortic occlusion; report of a case]. | fungal endocarditis caused by candida species is associated with high morbidity and mortality. a combination of surgical resection and antifungal drug therapy is the golden standard for treatment. we reported a case of fungal endocarditis due to candida lusitaniae found at onset of lower limb acute aortic occlusion cured by emergency operation. this case suggests that candida endocariditis can be managed medically with antifungal drug therapy in life time. | 2005 | 16104572 |
| identification of candida strains isolated from tanzanian pregnant women with vaginal candidiasis. | to identify candida strains isolated from tanzanian women (13 to 45 years) with vaginal candidiasis. | 2005 | 16119751 |
| development of a dna microarray for detection and identification of fungal pathogens involved in invasive mycoses. | invasive fungal infections have emerged as a major cause of morbidity and mortality in immunocompromised patients. conventional identification of pathogenic fungi in clinical microbiology laboratories is time-consuming and, therefore, often imperfect for the early initiation of an adequate antifungal therapy. we developed a diagnostic microarray for the rapid and simultaneous identification of the 12 most common pathogenic candida and aspergillus species. oligonucleotide probes were designed by ... | 2005 | 16207946 |
| yeast community survey in the tagus estuary. | the yeast community in the waters of the tagus estuary, portugal, was followed for over a year in order to assess its dynamics. yeast occurrence and incidence were measured and this information was related to relevant environmental data. yeast occurrence did not seem to depend upon tides, but river discharge had a dramatic impact both on the density and diversity of the community. the occurrence of some yeasts was partially correlated with faecal pollution indicators. yeast isolates were charact ... | 2005 | 16329949 |
| antimicrobial activity of flavonoids from pelargonium radula (cav.) l'hérit. | flavonoids from pelargonium radula (cav.) l'hérit were purified by column chromatography. two fractions were obtained: f1 (main flavonoid isoquercitrin) and f2 (main flavonoid rutin). in vitro antimicrobial activity of f1 and f2 were tested against eleven species of bacteria and eleven species of fungi. both fractions demonstrated strong inhibitory activity against staphylococcus aureus, proteus rettgeri, candida tropicalis and microsporum gypseum. staphylococcus sp. (coagulase-negative) and can ... | 2005 | 16375833 |
| in vitro pharmacodynamics of rapid versus continuous infusion of amphotericin b deoxycholate against candida species in the presence of human serum albumin. | recent open label studies have suggested that dosing amphotericin b (amb) by continuous infusion (ci) may reduce drug-associated infusion reactions and nephrotoxicity. in vitro and in vivo pharmacodynamic (pd) data, however, do not consistently support the concept of ci dosing based on the concentration-dependent activity of this agent and in vitro studies with amb rarely account for the drug's high degree of protein binding. therefore, we compared the pd activity of simulated continuous versus ... | 2006 | 16387749 |
| intrinsic in vitro susceptibility of primary clinical isolates of aspergillus fumigatus, aspergillus terreus, aspergillus nidulans, candida albicans and candida lusitaniae against amphotericin b. | a total of 60 clinical fungal isolates from patients without prior amphotericin b treatment and three control strains were evaluated for their intrinsic susceptibility to amphotericin b (amb) using microdilution, etest and disc diffusion assays, on three media each, roswell park memorial institute (rpmi) 1640, antibiotic medium 3 (am3) and high resolution medium. the fungal strains included isolates of aspergillus fumigatus (n = 10), aspergillus terreus (n = 12), aspergillus nidulans (n = 9), ca ... | 2006 | 16466441 |
| activity of novel non-amphipathic cationic antimicrobial peptides against candida species. | candida species are problematic opportunistic pathogens in the hospital setting, where they are frequently associated with opportunistic infections of indwelling medical devices. there are only a few effective classes of antifungal agents currently available, and some species, such as candida lusitaniae, candida glabrata and candida krusei, are intrinsically resistant to some of these drugs, further reducing existing therapeutic options. we have recently developed synthetic, non-amphipathic cati ... | 2006 | 16524895 |
| polymicrobial keratomycosis in a three-year-old child. | to describe a previously unreported case of polymicrobial mycotic keratitis caused by an association between candida lusitaniae, c. parapsilosis, and geotrichum candidum. | 2006 | 16597544 |
| phenotypic switching in candida lusitaniae on copper sulfate indicator agar: association with amphotericin b resistance and filamentation. | candida lusitaniae is an opportunistic yeast pathogen that has the ability to develop resistance to amphotericin b (amb). the mechanism(s) for this resistance is not well understood, although there are data supporting mutations in sterol pathways and other data supporting phenotypic switching (ps). the goal of this study was to determine whether c. lusitaniae has a ps system and to characterize any phenotypes, including any changes in amb mics. when 10(4) cfu of an amb-resistant (mic of 16 to 32 ... | 2006 | 16597887 |
| changing incidence of candida bloodstream infections among nicu patients in the united states: 1995-2004. | recent reports suggest that candidemia caused by fluconazole-resistant strains is increasing in certain adult populations. we evaluated the annual incidence of neonatal candidemia and the frequency of disease caused by different species of candida among neonates in the united states. | 2006 | 16651324 |
| novel lysine biosynthetic gene sequences (lys1 and lys5) used as pcr targets for the detection of the pathogenic candida yeast. | we report here a sensitive and specific polymerase chain reaction (pcr) detection assay for the pathogenic candida yeast based on the novel lys1 [encoding saccharopine dehydrogenase (sdh)] and lys5 [encoding phosphopantetheinyl transferase (pptase)] gene sequences of the fungal unique lysine biosynthetic pathway. both lys1 and lys5 dna-specific pcr primers sg1, sg2 and sg3, sg4, respectively, amplified predicted 483 and 648-bp fragments from candida albicans genomic dna but not from other select ... | 2006 | 16733732 |
| specificity of the monoclonal antibody 3h8 in the immunohistochemical identification of candida species. | candida albicans has been shown to be involved in the pathogenesis of adult periodontitis (ap). the diagnosis of candida-associated ap depends largely on the identification of yeast and pseudomycelial forms in gingival tissue samples by using periodic acid-schiff and gomori methenamine silver stains. however, these stains are non-specific and also reveal confusing artifacts seemingly rather difficult to distinguish from yeasts. with the recent development and availability of monoclonal antibodie ... | 2006 | 16792731 |
| candida lusitaniae and chronic granulomatous disease. | 2006 | 16874186 | |
| a fungal phylogeny based on 42 complete genomes derived from supertree and combined gene analysis. | to date, most fungal phylogenies have been derived from single gene comparisons, or from concatenated alignments of a small number of genes. the increase in fungal genome sequencing presents an opportunity to reconstruct evolutionary events using entire genomes. as a tool for future comparative, phylogenomic and phylogenetic studies, we used both supertrees and concatenated alignments to infer relationships between 42 species of fungi for which complete genome sequences are available. | 2006 | 17121679 |
| the discovery of moriniafungin, a novel sordarin derivative produced by morinia pestalozzioides. | a novel sordarin derivative, moriniafungin (1), containing a 2-hydroxysebacic acid residue linked to c-3' of the sordarose residue of sordarin through a 1,3-dioxolan-4-one ring was isolated from the fungus morinia pestalozzioides. isolation of moriniafungin employed a highly specific bioassay consisting of a panel of saccharomyces cerevisiae strains containing chimeric eef2 for candida glabrata, candida krusei, candida lusitaniae, crytpococcus neoformans, and aspergillus fumigatus as well as wil ... | 2006 | 16183294 |
| global surveillance of in vitro activity of micafungin against candida: a comparison with caspofungin by clsi-recommended methods. | micafungin is an echinocandin antifungal agent that has recently been approved for the prevention of invasive fungal infection and the treatment of esophageal candidiasis. prospective sentinel surveillance for the emergence of in vitro resistance to micafungin among invasive candida sp. isolates is indicated. we determined the in vitro activity of micafungin against 2,656 invasive (bloodstream or sterile site) unique patient isolates of candida spp. collected from 60 medical centers worldwide in ... | 2006 | 17021079 |
| [restropective investigation about the treatment of candidaemia in a french university hospital in 2004]. | this study aimed to retrospectively evaluate the adequate use of antifungal drugs in the treatment of the candidaemia. a collection of clinical, biological and therapeutic data was carried out for the patients who had a positive blood culture for candida species during the year 2004. the antifungal therapy was compared to the guidelines of the french conference of consensus named "prise en charge des candidoses et aspergilloses invasives de l'adulte". the degree of conformity was classified as f ... | 2006 | 17045421 |
| molecular mechanism of flucytosine resistance in candida lusitaniae: contribution of the fcy2, fcy1, and fur1 genes to 5-fluorouracil and fluconazole cross-resistance. | inactivation of the fcy2 (cytosine permease), fcy1 (cytosine deaminase), and fur1 (uracil phosphoribosyltransferase) genes in candida lusitaniae produced two patterns of resistance to flucytosine. mutant fur1 demonstrated resistance to 5-fluorouracil, whereas mutants fcy1 and fcy2 demonstrated fluconazole resistance in the presence of subinhibitory flucytosine concentrations. | 2007 | 17060521 |
| fluconazole susceptibility testing of candida species by flow cytometry. | currently, antifungal drug susceptibility testing is labor-intensive, limited by delays in obtaining results and high costs. the purpose of this study was to determine the usefulness of flow cytometry (fcm) antifungal drug susceptibility testing as a routine laboratory procedure. | 2007 | 17084902 |
| one year prospective survey of candida bloodstream infections in scotland. | a 12 month survey of candidaemia in scotland, uk, in which every scottish hospital laboratory submitted all blood isolates of yeasts for identification, strain typing and susceptibility testing, provided 300 isolates from 242 patients, generating incidence data of 4.8 cases per 100,000 population per year and 5.9 cases per 100,000 acute occupied bed days; 27.9 % of cases occurred in intensive care units. more than half the patients with candidaemia had an underlying disease involving the abdomen ... | 2007 | 17644714 |
| differential involvement of histidine kinase receptors in pseudohyphal development, stress adaptation, and drug sensitivity of the opportunistic yeast candida lusitaniae. | fungal histidine kinase receptors (hkrs) sense and transduce many extracellular signals. we investigated the role of hkrs in morphogenetic transition, osmotolerance, oxidative stress response, and mating ability in the opportunistic yeast candida lusitaniae. we isolated three genes, sln1, nik1, and chk1, potentially encoding hkrs of classes vi, iii, and x, respectively. these genes were disrupted by a transformation system based upon the "ura3 blaster" strategy. functional analysis of disruptant ... | 2007 | 17660361 |
| candida lusitaniae arthritis in an intravenous drug user. | a case of arthritis of the right knee caused by candida lusitaniae in a 29-year-old intravenous drug abuser is described. the diagnosis was based on the isolation of c. lusitaniae from synovial fluid and was supported by the presence of c. lusitaniae-specific dna and high levels of (1-3)-beta-d-glucan (122 pg ml-1) in the same specimen. while the isolate was susceptible to amphotericin b and fluconazole in vitro, treatment with amphotericin b was not very effective. the patient achieved complete ... | 2007 | 17714366 |
| dna microarray-based detection and identification of fungal pathogens in clinical samples from neutropenic patients. | the increasing incidence of invasive fungal infections (ifi) in immunocompromised patients emphasizes the need to improve diagnostic tools. we established a dna microarray to detect and identify dna from 14 fungal pathogens (aspergillus fumigatus, aspergillus flavus, aspergillus terreus, candida albicans, candida dubliniensis, candida glabrata, candida lusitaniae, candida tropicalis, fusarium oxysporum, fusarium solani, mucor racemosus, rhizopus microsporus, scedosporium prolificans, and trichos ... | 2007 | 17715373 |
| changing pattern of candidaemia 2001-2006 and use of antifungal therapy at the university hospital of vienna, austria. | a retrospective survey of candidaemia between 2001 and 2006 was performed at the university hospital of vienna, a 2200-bed centre with large organ transplantation and haematology-oncology units. the incidence rate of candida spp. in blood cultures increased from 0.27 cases/1000 admissions in 2001 to 0.77 cases/1000 admissions in 2006 (p <0.005). the incidence of candidaemia caused by candida albicans and by non-albicans candida spp. both increased during this period; although there was a trend t ... | 2007 | 17725647 |
| the pan-ac assay: a single-reaction real-time pcr test for quantitative detection of a broad range of aspergillus and candida species. | in view of the growing incidence and the high mortality of invasive aspergillosis and candidiasis, adequate diagnostic techniques permitting timely onset of treatment are of paramount importance. more than 90 % of all invasive fungal infections in immunocompromised individuals can be attributed to candida and aspergillus species. to date, standardized techniques permitting rapid, sensitive and, no less importantly, economic screening for the clinically most relevant fungi are lacking. in the pre ... | 2007 | 17761478 |
| effect of lemon extract on foodborne microorganisms. | a quantitative investigation was conducted on the antimicrobial effect of lemon extract against some food spoilage microorganisms: yeasts, bacillus species, and lactic acid bacteria. growth kinetics and dose-response profiles were determined from experimental data obtained with a suitable macrodilution methodology based on a turbidimetric technique. growth and no-growth status of microbial suspensions were expressed in terms of noninhibitory concentration (nic) and mic. lemon extract was effecti ... | 2007 | 17803147 |
| correlation of posaconazole minimum fungicidal concentration and time kill test against nine candida species. | we evaluated the in vitro activity of posaconazole against nine candida species using minimum fungicidal concentration (mfc) measurements and time-kill methods. | 2007 | 17875607 |
| [the fungicidal activity and paradoxical effect of caspofungin against yeast. influence of culture medium and incubation time]. | the effect of culture medium and incubation time on the fungicidal activity of caspofungin and on the frequency of paradoxical effect has been studied in 144 blood culture isolates (42, candida tropicalis, 32 candida glabrata, 21 candida krusei, 20 candida parapsilosis, 11 candida guilliermondii, 4 candida famata, 3 candida lusitaniae, 3 blastoschizomyces capitatus, 2 saccharomyces cerevisiae, 2 yarrowia lipolytica, 1 candida inconspicua, 1 candida lambica, 1 candida sake y 1 pichia ohmeri). the ... | 2007 | 18563217 |
| development of primers for detecting dominant yeasts in smear-ripened cheeses. | pcr primers were developed for the specific detection of clavispora lusitaniae, debaryomyces hansenii var hansenii, geotrichum candidum, kluyveromyces lactis and k. marxianus and yarrowia lipolytica, yeast species commonly found on the surface of smear cheese. forty eight representative strains frequently found in smear cheeses or taxonomically related to the target yeasts were used as templates, to validate the designed primers. the specific and selective detection of these yeasts was effective ... | 2007 | 17291384 |
| stability of the biodiversity of the surface consortia of gubbeen, a red-smear cheese. | a total of 1,052 bacteria and 828 yeasts were isolated from the surface flora of 6 batches of gubbeen cheese made in 1996-1997 and 2002-2003. stability of the microflora was evaluated over time and also during ripening at 4, 10, and 16 d (batches 4, 5, and 6) or at 4, 16, 23, and 37 d (batches 1, 2, and 3). bacteria were identified using pulsed-field gel electrophoresis, repetitive extragenic palindromic-pcr, and 16s rrna gene sequencing, and yeasts were identified by fourier transform infrared ... | 2007 | 17430918 |
| differentiation of candida albicans from non-albicans yeast directly from blood cultures by gram stain morphology. | clusters of pseudohyphae are commonly seen on gram stain of blood cultures from patients with candida albicans fungemia. whether this morphologic feature is useful for differentiating c. albicans from other yeasts has not previously been systematically evaluated. yeast morphology on gram stain of blood cultures from consecutive patients with fungemia detected by the bactec automated blood culture system was prospectively assessed and correlated with the final culture-based yeast identification. ... | 2007 | 17447090 |
| surface charge influences enterococcal prevalence in mixed-species biofilms. | to study the influence of 15 microbial isolates on the prevalence of charge-heterogeneous and charge-homogeneous enterococcus faecalis strains, all isolated from biliary stents, in mixed-species biofilms. | 2007 | 17448160 |
| species identification and strain differentiation of clinical candida isolates using the diversilab system of automated repetitive sequence-based pcr. | the diversilab system, which uses repetitive sequence-based pcr (rep-pcr) to genotype micro-organisms, was evaluated as a molecular typing tool for members of the genus candida. initially, 41 clinical candida spp. (7 candida krusei, 10 candida parapsilosis, 7 candida albicans, 10 candida tropicalis and 7 candida glabrata), previously identified at the species level by morphological and biochemical analysis, were analysed with the diversilab system. species identification was confirmed by dna seq ... | 2007 | 17510263 |
| ce separation of proteins and yeasts dynamically modified by peg pyrenebutanoate with fluorescence detection. | the optimized protocols of the bioanalytes separation, proteins and yeasts, dynamically modified by the nonionogenic tenside peg pyrenebutanoate, were applied in cze and cief with the acidic gradient in ph range 2-5.5, both with fluorescence detection. peg pyrenebutanoate was used as a buffer additive for a dynamic modification of proteins and/or yeast samples. the narrow peaks of modified analytes were detected. the values of the pi's of the labeled proteins were calculated using new fluorescen ... | 2007 | 17557360 |
| [dacryocystitis caused by candida lusitaniae]. | case report: we report the case of a 60-year-old woman with recurrent dacryocystitis of the right lacrimal sac. she did not recover after medical treatment, nor after dacryocystorhinostomy with canalicular intubation. she also had dacryocystitis on the left side. the material that was obtained during surgery was grown in a culture medium and candida lusitaniae was identified as the responsible organism. all symptoms disappeared after topical treatment with amphotericin b and dacryocystorhinostom ... | 2007 | 17573647 |
| comparison of a randomly amplified polymorphic dna (rapd) analysis and atb id 32c system for identification of clinical isolates of different candida species. | the objective of this work was to compare the usefulness of a randomly amplified polymorphic dna (rapd) assay to that of the atb id32c kit (biomérieux, france) for identification of different species of candida isolated from clinical specimens. the rapd-pcr patterns obtained with ope-18 primer for identification of clinical isolates were consistent, and the different independent assays revealed reproduction of the band patterns. rapd with the ope-18 primer is a very specific and sensitive method ... | 2007 | 17604435 |
| evidence of recent interkingdom horizontal gene transfer between bacteria and candida parapsilosis. | to date very few incidences of interdomain gene transfer into fungi have been identified. here, we used the emerging genome sequences of candida albicans wo-1, candida tropicalis, candida parapsilosis, clavispora lusitaniae, pichia guilliermondii, and lodderomyces elongisporus to identify recent interdomain hgt events. we refer to these as ctg species because they translate the ctg codon as serine rather than leucine, and share a recent common ancestor. | 2008 | 18577206 |
| identification of yeasts isolated from commercial shell eggs stored at refrigerated temperatures. | yeasts and molds can grow on or in eggs, causing spoilage. washed and unwashed eggs (treatments) were collected aseptically on three separate days (replications) from a commercial processing facility and stored for 10 weeks at 4 degrees c. ten eggs from each treatment were sampled weekly (110 eggs per treatment per replication). yeasts and molds were enumerated from external shell rinses by plating onto acidified potato dextrose agar. yeast colonies were picked randomly and stored for subsequent ... | 2008 | 18592756 |
| debaryomyces hansenii (candida famata), a rare human fungal pathogen often misidentified as pichia guilliermondii (candida guilliermondii). | debaryomyces hansenii is a hemiascomycetous yeast commonly found in natural substrates and in various types of cheese. pichia guilliermondii is widely distributed in nature and is a common constituent of the normal human microflora. both species have been described in human infections but are extremely difficult to differentiate phenotypically. thus, frequent errors in identification occur. the 62 clinical and environmental isolates sent between 2000 and 2007 to the french national reference cen ... | 2008 | 18701668 |