Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| crystal structure of a lactosucrose-producing enzyme, arthrobacter sp. k-1 β-fructofuranosidase. | arthrobacter sp. k-1 β-fructofuranosidase (arffase), a glycoside hydrolase family 68 enzyme, catalyzes the hydrolysis and transfructosylation of sucrose. arffase is useful for producing a sweetener, lactosucrose (4(g)-β-d-galactosylsucrose). the primary structure of arffase is homologous to those of levansucrases, although arffase catalyzes mostly hydrolysis when incubated with sucrose alone, even at high concentration. here, we determined the crystal structure of arffase in unliganded form and ... | 2012 | 23040392 |
| essential role of the czc determinant for cadmium, cobalt and zinc resistance in gluconacetobacter diazotrophicus pal 5. | the mechanisms of cadmium, cobalt and zinc resistance were characterized in the plant-growth-promoting bacterium gluconacetobacter diazotrophicus pal 5. the resistance level of the wild-type strain was evaluated through the establishment of minimum inhibitory concentrations (mic) of the soluble compounds cdcl2·h2o, cocl2·6h2o and zncl2. gluconacetobacter diazotrophicus pal 5 was resistant to high concentrations of cd, co and zn, with mics of 1.2, 20 and 20 mm, respectively. screening of an inser ... | 2012 | 22847268 |
| identification and validation of reference genes to study the gene expression in gluconacetobacter diazotrophicus grown in different carbon sources using rt-qpcr. | gluconacetobacter diazotrophicus strain pal5 is a nitrogen-fixing endophytic bacterium originally isolated from sugarcane and later on was found to colonize other plants such as rice, elephant grass, sweet potato, coffee, and pineapple. currently, g. diazotrophicus has been considered a plant growth-promoting bacterium due to its characteristics of biological nitrogen fixation, phytohormone secretion, solubilization of mineral nutrients and antagonism to phytopathogens. reverse transcription fol ... | 2012 | 22814372 |
| identification of n-acyl homoserine lactones produced by gluconacetobacter diazotrophicus pal5 cultured in complex and synthetic media. | the endophytic diazotrophic gluconacetobacter diazotrophicus pal5 was originally isolated from sugarcane (saccharum officinarum). the biological nitrogen fixation, phytohormones secretion, solubilization of mineral nutrients and phytopathogen antagonism allow its classification as a plant growth-promoting bacterium. the recent genomic sequence of pal5 unveiled the presence of a quorum sensing (qs) system. qs are regulatory mechanisms that, through the production of signal molecules or autoinduce ... | 2012 | 22350020 |
| in vitro and in vivo inoculation of four endophytic bacteria on lycopersicon esculentum. | four bacteria selected on the basis of their capability of fixing atmospheric nitrogen, stimulating plant-growth, and protecting the host plant from pathogens - azospirillum brasilense, gluconacetobacter diazotrophicus, herbaspirillum seropedicae, burkholderia ambifaria - were inoculated on tomato seeds either singularly, in couple and in a four bacteria mixer. aim of this research was to evaluate: (1) effect of single and mixed cultures on the inoculated plant - plant growth, dry weight, root l ... | 2013 | 23352842 |
| biochemical and molecular characterisation of the bacterial endophytes from native sugarcane varieties of himalayan region. | seven endophytic bacterial isolates were finally recovered from native sugarcane varieties at hilly areas namely berinag, champawat and didihat of uttarakhand state in northern himalayan region. new isolates and two standard cultures-azospirillum brasilense and gluconacetobacter diazotrophicus, were evaluated for their morphological, biochemical and molecular characteristics. morphologically all were rod shaped, gram-negative bacteria. their plant growth promotory properties were also assessed w ... | 2013 | 28324369 |
| structural studies of an exopolysaccharide produced by gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium that has been found colonizing several plants. this acid-tolerant bacterium produces phytohormones that promote plant growth and is also able to grow in high-sugar concentrations. it has been demonstrated that exopolysaccharides (eps), which are produced by strain pal5 of g. diazotrophicus, play an important role in plant infection. we have investigated the structure of the eps, which was produced by a strain of pal5 grown in liquid ... | 2013 | 23987457 |
| the bacterial superoxide dismutase and glutathione reductase are crucial for endophytic colonization of rice roots by gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus is an aerobic diazotrophic plant-growth-promoting bacterium isolated from different gramineous plants. we showed that reactive oxygen species (ros) were produced at early stages of rice root colonization, a typical plant defense response against pathogens. the transcription of the pathogen-related-10 gene of the jasmonic acid (ja) pathway but not of the pr-1 gene of the salicylic acid pathway was activated by the endophytic colonization of rice roots by g. diazot ... | 2013 | 23634840 |
| identification and characterization of an iron abc transporter operon in gluconacetobacter diazotrophicus pal 5. | gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium and endophyte of sugarcane. we have cloned and sequenced the genes coding for the components of the iron abc-type acquisition system of g. diazotrophicus. sequence analysis revealed three orfs, (feua, feub, and feuc) organized as an operon and encoding polypeptides of 346 (38 kda), 342 (34.2 kda), and 240 (26 kda) amino acids, respectively. the deduced translation products of the feu operon showed similarity with a periplasmic solut ... | 2013 | 23624722 |
| gluconic acid produced by gluconacetobacter diazotrophicus pal5 possesses antimicrobial properties. | gluconic acid is produced in large quantities by the endophytic and diazotrophic bacterium gluconacetobacter diazotrophicus pal5. this organic acid derives from direct oxidation of glucose by a pyrroloquinoline-quinone-linked glucose dehydrogenase in this plant growth-promoting bacterium. in the present article, evidence is presented showing that gluconic acid is also responsible for the antimicrobial activity of g. diazotrophicus pal5. the broad antagonistic spectrum includes gram-positive and ... | 2014 | 25049167 |
| gluconacetobacter diazotrophicus pal5 possesses an active quorum sensing regulatory system. | the endophytic bacterium gluconacetobacter diazotrophicus colonizes a broad range of host plants. its plant growth-promoting capability is related to the capacity to perform biological nitrogen fixation, the biosynthesis of siderophores, antimicrobial substances and the solubilization of mineral nutrients. colonization of and survival in these endophytic niche requires a complex regulatory network. among these, quorum sensing systems (qs) are signaling mechanisms involved in the control of sever ... | 2014 | 24974195 |
| structure and functional characterization of pyruvate decarboxylase from gluconacetobacter diazotrophicus. | bacterial pyruvate decarboxylases (pdc) are rare. their role in ethanol production and in bacterially mediated ethanologenic processes has, however, ensured a continued and growing interest. pdcs from zymomonas mobilis (zmpdc), zymobacter palmae (zppdc) and sarcina ventriculi (svpdc) have been characterized and zmpdc has been produced successfully in a range of heterologous hosts. pdcs from the acetobacteraceae and their role in metabolism have not been characterized to the same extent. examples ... | 2014 | 25369873 |
| drought tolerance conferred to sugarcane by association with gluconacetobacter diazotrophicus: a transcriptomic view of hormone pathways. | sugarcane interacts with particular types of beneficial nitrogen-fixing bacteria that provide fixed-nitrogen and plant growth hormones to host plants, promoting an increase in plant biomass. other benefits, as enhanced tolerance to abiotic stresses have been reported to some diazotrophs. here we aim to study the effects of the association between the diazotroph gluconacetobacter diazotrophicus pal5 and sugarcane cv. sp70-1143 during water depletion by characterizing differential transcriptome pr ... | 2014 | 25489849 |
| phosphate enhances levan production in the endophytic bacterium gluconacetobacter diazotrophicus pal5. | gluconacetobacter diazotrophicus is a gram-negative and endophytic nitrogen-fixing bacterium that has several beneficial effects in host plants; thus, utilization of this bacterium as a biofertilizer in agriculture may be possible. g. diazotrophicus synthesizes levan, a d-fructofuranosyl polymer with β-(2→6) linkages, as an exopolysaccharide and the synthesized levan improves the stress tolerance of the bacterium. in this study, we found that phosphate enhances levan production by g. diazotrophi ... | 2014 | 24717418 |
| pinus flexilis and picea engelmannii share a simple and consistent needle endophyte microbiota with a potential role in nitrogen fixation. | conifers predominantly occur on soils or in climates that are suboptimal for plant growth. this is generally attributed to symbioses with mycorrhizal fungi and to conifer adaptations, but recent experiments suggest that aboveground endophytic bacteria in conifers fix nitrogen (n) and affect host shoot tissue growth. because most bacteria cannot be grown in the laboratory very little is known about conifer-endophyte associations in the wild. pinus flexilis (limber pine) and picea engelmannii (eng ... | 2014 | 25071746 |
| the oxidative fermentation of ethanol in gluconacetobacter diazotrophicus is a two-step pathway catalyzed by a single enzyme: alcohol-aldehyde dehydrogenase (adha). | gluconacetobacter diazotrophicus is a n2-fixing bacterium endophyte from sugar cane. the oxidation of ethanol to acetic acid of this organism takes place in the periplasmic space, and this reaction is catalyzed by two membrane-bound enzymes complexes: the alcohol dehydrogenase (adh) and the aldehyde dehydrogenase (aldh). we present strong evidence showing that the well-known membrane-bound alcohol dehydrogenase (adha) of ga. diazotrophicus is indeed a double function enzyme, which is able to use ... | 2015 | 25574602 |
| nitrogen fixing bacteria in the family acetobacteraceae and their role in agriculture. | for centuries, the acetobacteraceae is known as a family that harbors many species of organisms of biotechnological importance for industry. nonetheless, since 1988 representatives of this family have also been described as nitrogen fixing bacteria able to plant growth promotion by a variety of mechanisms. nitrogen fixation is a biological process that guarantees that the atmospheric n2 is incorporated into organic matter by several bacterial groups. most representatives of this group, also know ... | 2015 | 25736602 |
| tn5 insertion in the tonb gene promoter affects iron-related phenotypes and increases extracellular siderophore levels in gluconacetobacter diazotrophicus. | tonb-dependent receptors in concert with the tonb-exbb-exbd protein complex are responsible for the uptake of iron and substances such as vitamin b12 in several bacterial species. in this study, tn5 mutagenesis of the sugarcane endophytic bacterium gluconacetobacter diazotrophicus led to the isolation of a mutant with a single tn5-insertion in the promoter region of a tonb gene ortholog. this mutant, named gdiaa31, displayed a reduced growth rate and a lack of response to iron availability when ... | 2015 | 25323530 |
| the crystal structure of erwinia amylovora levansucrase provides a snapshot of the products of sucrose hydrolysis trapped into the active site. | levansucrases are members of the glycoside hydrolase family and catalyse both the hydrolysis of the substrate sucrose and the transfer of fructosyl units to acceptor molecules. in the presence of sufficient sucrose, this may either lead to the production of fructooligosaccharides or fructose polymers. aim of this study is to rationalise the differences in the polymerisation properties of bacterial levansucrases and in particular to identify structural features that determine different product sp ... | 2015 | 26208466 |
| purification and characterization of the membrane-bound quinoprotein glucose dehydrogenase of gluconacetobacter diazotrophicus pal 5. | acetic acid bacteria oxidize a great number of substrates, such as alcohols and sugars, using different enzymes that are anchored to the membrane. in particular, gluconacetobacter diazotrophicus is distinguished for its n2-fixing activity under high-aeration conditions. ga. diazotrophicus is a true endophyte that also has membrane-bound enzymes to oxidize sugars and alcohols. here we reported the purification and characterization of the membrane-bound glucose dehydrogenase (gdhm), an oxidoreduct ... | 2015 | 25576305 |
| tyrosine-coordinated p-cluster in g. diazotrophicus nitrogenase: evidence for the importance of o-based ligands in conformationally gated electron transfer. | the p-cluster is a unique iron-sulfur center that likely functions as a dynamic electron (e(-)) relay site between the fe-protein and the catalytic femo-cofactor in nitrogenase. the p-cluster has been shown to undergo large conformational changes upon 2-e(-) oxidation which entail the coordination of two of the fe centers to a ser side chain and a backbone amide n, respectively. yet, how and if this 2-e(-) oxidized state (p(ox)) is involved in catalysis by nitrogenase is not well established. he ... | 2016 | 27487256 |
| isolation and characterization of active promoters from gluconacetobacter diazotrophicus strain pal5 using a promoter-trapping plasmid. | gluconacetobacter diazotrophicus is a nitrogen-fixing, endophytic bacterium that has the potential to promote plant growth and increase yield. genetically modified strains might get more benefits to host plants, including through expression of useful proteins, such as cry toxins from b. thuringiensis, or enzymes involved in phytohormone production, proteins with antagonistic activity for phytopathogens, or that improve nutrient utilization by the plant. for that, expression systems for g. diazot ... | 2016 | 26914247 |
| differential effects of salinity and osmotic stress on the plant growth-promoting bacterium gluconacetobacter diazotrophicus pal5. | plant growth-promoting bacteria (pgpb) represent a promising alternative to the massive use of industrial fertilizers in agriculture. gluconacetobacter diazotrophicus is a pgpb that colonizes several plant species. although this bacterium is able to grow at high sucrose concentrations, its response to environmental stresses is poorly understood. the present study evaluated g. diazotrophicus pal5 response to stresses caused by sucrose, peg 400, nacl, kcl, na2so4 and k2so4. morphological, ultrastr ... | 2016 | 26809283 |
| the decrease in the population of gluconacetobacter diazotrophicus in sugarcane after nitrogen fertilization is related to plant physiology in split root experiments. | it has been established that a decrease in the population of gluconacetobacter diazotrophicus associated with sugarcane occurs after nitrogen fertilization. this fact could be due to a direct influence of nh(4)no(3) on bacterial cells or to changes in plant physiology after fertilizer addition, affecting bacterial establishment. in this work, we observed that survival of g. diazotrophicus was directly influenced when 44.8mm of nh(4)no(3) (640mgn/plant) was used for in vitro experiments. furtherm ... | 2016 | 26652262 |
| identification of genes involved in indole-3-acetic acid biosynthesis by gluconacetobacter diazotrophicus pal5 strain using transposon mutagenesis. | gluconacetobacter diazotrophicus is a beneficial nitrogen-fixing endophyte found in association with sugarcane plants and other important crops. beneficial effects of g. diazotrophicus on sugarcane growth and productivity have been attributed to biological nitrogen fixation process and production of phytohormones especially indole-3-acetic acid (iaa); however, information about the biosynthesis and function of iaa in g. diazotrophicus is still scarce. therefore, the aim of this work was to ident ... | 2016 | 27774087 |
| dissection of exopolysaccharide biosynthesis in kozakia baliensis. | acetic acid bacteria (aab) are well known producers of commercially used exopolysaccharides, such as cellulose and levan. kozakia (k.) baliensis is a relatively new member of aab, which produces ultra-high molecular weight levan from sucrose. throughout cultivation of two k. baliensis strains (dsm 14400, nbrc 16680) on sucrose-deficient media, we found that both strains still produce high amounts of mucous, water-soluble substances from mannitol and glycerol as (main) carbon sources. this indica ... | 2016 | 27716345 |
| the shifts of diazotrophic communities in spring and summer associated with coral galaxea astreata, pavona decussata, and porites lutea. | the coral holobiont often resides in oligotrophic waters; both coral cells and their symbiotic dinoflagellates possess ammonium assimilation enzymes and potentially benefit from the nitrogen fixation of coral-associated diazotrophs. however, the seasonal dynamics of coral-associated diazotrophs are not well characterized. here, the seasonal variations of diazotrophic communities associated with three corals, galaxea astreata, pavona decussata, and porites lutea, were studied using nifh gene ampl ... | 2016 | 27920768 |
| development and nitrate reductase activity of sugarcane inoculated with five diazotrophic strains. | diazotrophs are able to stimulate plant growth. this study aimed at evaluating the effect of inoculation of five diazotrophic strains on growth promotion and nitrate reductase (nr, ec 1.7.1.1) activity in sugarcane. an experiment was carried out from three stages of cultivation: sprouting, tubes, and in hydroponics. on the first two stages, seven treatments were adopted: uninoculated control; mixed inoculation with five strains; and individual inoculation with gluconacetobacter diazotrophicus (g ... | 2017 | 28321482 |
| essential role of k(+) uptake permease (kup) for resistance to sucrose-induced stress in gluconacetobacter diazotrophicus pal 5. | microorganisms are constantly challenged by stressful conditions, such as sugar-rich environments. such environments can cause an imbalance of biochemical activities and compromise cell multiplication. gluconacetobacter diazotrophicus pal 5 is among the most sugar-tolerant bacteria, capable of growing in the presence of up to 876 mm sucrose. however, the molecular mechanisms involved in its response to high sucrose remain unknown. the present work aimed to identify sucrose-induced stress resista ... | 2017 | 27886654 |