Publications

insights on the evolution of mycoparasitism from the genome of clonostachys rosea.	clonostachys rosea is a mycoparasitic fungus that can control several important plant diseases. here, we report on the genome sequencing of c. rosea and a comparative genome analysis, in order to resolve the phylogenetic placement of c. rosea and to study the evolution of mycoparasitism as a fungal lifestyle. the genome of c. rosea is estimated to 58.3 mb, and contains 14,268 predicted genes. a phylogenomic analysis shows that c. rosea clusters as sister taxon to plant pathogenic fusarium specie ...	2015	25575496
identification of mycoparasitism-related genes in clonostachys rosea 67-1 active against sclerotinia sclerotiorum.	clonostachys rosea is a mycoparasite that has shown great potential in controlling various plant fungal pathogens. in order to find mycoparasitism-related genes in c. rosea, the transcriptome of the efficient isolate 67-1 in association with sclerotia of sclerotinia sclerotiorum was sequenced and analysed. the results identified 26,351 unigenes with a mean length of 1,102 nucleotides, among which 18,525 were annotated in one or more databases of nr, kegg, swiss-prot, go and cog. differentially e ...	2015	26657839
efficacy of clonostachys rosea and duddingtonia flagrans in reducing the haemonchus contortus infective larvae.	the biocontrol is proven effective in reducing in vitro and in situ free-living stages of major gastrointestinal helminths, allowing progress in reducing losses by parasitism, maximizing production, and productivity. this study aimed at evaluating the predatory activity of fungal isolates of duddingtonia flagrans and clonostachys rosea species and its association on infective larvae (l3) of h. contortus in microplots formed by grasses and maintained in a protected environment. all groups were ad ...	2015	26504809
fungal antagonism assessment of predatory species and producers metabolites and their effectiveness on haemonchus contortus infective larvae.	the objective of this study was to assess antagonism of nematophagous fungi and species producers metabolites and their effectiveness on haemonchus contortus infective larvae (l3). assay a assesses the synergistic, additive, or antagonistic effect on the production of spores of fungal isolates of the species duddingtonia flagrans, clonostachys rosea, trichoderma esau, and arthrobotrys musiformis; assay b evaluates in vitro the effect of intercropping of these isolates grown in 2% water-agar (2% ...	2015	26504791
hazard identification of cis/trans-zearalenone through the looking-glass.	among the food-related health issues, the presence of contaminants has a prominent role, due to the wide range of exogenous compounds that can occur in food commodities and to their large differences in structure and biological activity. a comprehensive assessment of the related risk is thus actually demanding in terms of time and facilities involved. in this context, the use of computational strategies can be an effective choice for supporting the hazard identification procedure at the early st ...	2015	26391124
dual effects of metarhizium spp. and clonostachys rosea against an insect and a seed-borne pathogen in wheat.	crops are often prone to both insect herbivory and disease, which necessitate multiple control measures. ideally, an efficacious biological control agent must adequately control the target organism and not be inhibited by other biological control agents when applied simultaneously. wheat seeds infected with the plant pathogen fusarium culmorum were treated with metarhizium brunneum or m. flavoviride and clonostachys rosea individually and in combination, with the expectation to control both root ...	2016	25827357
clonostachys rosea reduces spot blotch in barley by inhibiting prepenetration growth and sporulation of bipolaris sorokiniana without inducing resistance.	several diseases threaten cereal production, and fungicides are therefore widely used. biological control is an environmentally friendly alternative, and the fungus clonostachys rosea is a versatile antagonist, effective against several plant diseases. we studied the ability of c. rosea to control barley leaf pathogens and the mechanisms behind the inhibition, emphasising induced resistance.	2016	26910381
draft genome sequence of fungus clonostachys rosea strain ykd0085.	here, we report the draft genome sequence of clonostachys rosea (strain ykd0085). the functional annotation of c. rosea provides important information related to its ability to produce secondary metabolites. the genome sequence presented here builds the basis for further genome mining.	2016	27340057
isolation of secondary metabolites from the soil-derived fungus clonostachys rosea yrs-06, a biological control agent, and evaluation of antibacterial activity.	the fungus clonostachys rosea is widely distributed all over the world. the destructive force of this fungus, as a biological control agent, is very strong to lots of plant pathogenic fungi. as part of the ongoing search for antibiotics from fungi obtained from soil samples, the secondary metabolites of c. rosea yrs-06 were investigated. through efficient bioassay-guided isolation, three new bisorbicillinoids possessing open-ended cage structures, tetrahydrotrichodimer ether (1) and dihydrotrich ...	2016	26974009
clonostachys rosea demethiolase str3 controls the conversion of methionine into methanethiol.	eukaryote-derived methioninase, catalyzing the one-step degradation of methionine (met) to methanethiol (mtl), has received much attention for its low immunogenic potential and use as a therapeutic agent against met-dependent tumors. although biological and chemical degradation pathways for met-mtl conversion are proposed, the concrete molecular mechanism for met-mtl conversion in eukaryotes is still unclear. previous studies demonstrated that α-keto-methylthiobutyric acid (kmba), the intermedia ...	2016	26902928
regulating ehrlich and demethiolation pathways for alcohols production by the expression of ubiquitin-protein ligase gene huwe1.	ehrlich and demethiolation pathways as two competing branches converted amino acid into alcohols. controlling both pathways offers considerable potential for industrial applications including alcohols overproduction, flavor-quality control and developing new flavors. while how to regulate ehrlich and demethiolation pathways is still not applicable. taking the conversion of methionine into methionol and methanethiol for example, we constructed two suppression subtractive cdna libraries of clonost ...	2016	26860895
investigating the compatibility of the biocontrol agent clonostachys rosea ik726 with prodigiosin-producing serratia rubidaea s55 and phenazine-producing pseudomonas chlororaphis toza7.	this study was carried out to assess the compatibility of the biocontrol fungus clonostachys rosea ik726 with the phenazine-producing pseudomonas chlororaphis toza7 or with the prodigiosin-producing serratia rubidaea s55 against fusarium oxysporum f. sp. radicis-lycopersici. the pathogen was inhibited by both strains in vitro, whereas c. rosea displayed high tolerance to s. rubidaea but not to p. chlororaphis. we hypothesized that this could be attributed to the atp-binding cassette (abc) protei ...	2016	26860841
transaldolase gene tal67 enhances the biocontrol activity of clonostachys rosea 67-1 against sclerotinia sclerotiorum.	clonostachys rosea is a promising biocontrol agent that parasitizes various fungal plant pathogens. in this paper, transaldolase gene tal67 was found to be greatly upregulated in c. rosea isolate 67-1 during mycoparasitism of sclerotinia sclerotiorum sclerotia. quantitative real-time pcr revealed a significant increase in expression at 0-48 h after induction by sclerotia, and the level peaked at 13.9-fold higher than the control at 24 h. gene disruption led to a decrease in the growth rate of th ...	2016	27130824
the abc transporter abcg29 is involved in h2o2 tolerance and biocontrol traits in the fungus clonostachys rosea.	for successful biocontrol interactions, biological control organisms must tolerate toxic metabolites produced by themselves or plant pathogens during mycoparasitic/antagonistic interactions, by host plant during colonization of the plant, and xenobiotics present in the environment. atp-binding cassette (abc) transporters can play a significant role in tolerance of toxic compounds by mediating active transport across the cellular membrane. this paper reports on functional characterization of an a ...	2016	26520102
interactions among filamentous fungi aspergillus niger, fusarium verticillioides and clonostachys rosea: fungal biomass, diversity of secreted metabolites and fumonisin production.	interactions among fungi colonizing dead organic matter involve exploitation competition and interference competition. major mechanism of interference competition is antibiosis caused by secreted secondary metabolites. the effect of competition on secondary metabolite production by fungi is however poorly understood. fungal biomass was rarely monitored in interaction studies; it is not known whether dominance in pairwise interactions follows congruent patterns.	2016	27165654
necrotrophic mycoparasites and their genomes.	mycoparasitism is a lifestyle where one fungus establishes parasitic interactions with other fungi. species of the genus trichoderma together with clonostachys rosea are among the most studied fungal mycoparasites. they have wide host ranges comprising several plant pathogens and are used for biological control of plant diseases. trichoderma as well as c. rosea mycoparasites efficiently overgrow and kill their fungal prey by using infection structures and by applying lytic enzymes and toxic meta ...	2017	28281442
insect deterrent and nematicidal screening of microfungi from mexico and anti-aphid compounds from gliomastix masseei.	fungal metabolites are promising alternatives for the development of biorational pesticides. in this sense, microfungi from tropical regions are valuable sources of natural compounds for pest management. with the aim of broadening the search for new eco-friendly products to manage plant pests, this study was carried out to evaluate the biological activity of 23 tropical fungal extracts on three species of phytophagous insects and a plant parasitic nematode. in addition, the active principles of ...	2017	28258772
efficacy of fungal and bacterial antagonists for controlling growth, fum1 gene expression and fumonisin b1 production by fusarium verticillioides on maize cobs of different ripening stages.	this study was carried out to examine the efficacy of two biocontrol agents (clonostachys rosea 016, bca1; gram-negative bacterium, bca5) for control of fum1 gene expression and fumonisin b1 (fb1) production by f. verticillioides fv1 on maize cobs of different ripening stages: r3, milk (0.985 aw); r4, dough (0.976 aw); r5, dent (0.958 aw). initially, temporal studies on fum1 gene expression and fb1 production were performed on maize kernels for up to 14days. this revealed that day 10 was optimum ...	2017	28213318
predatory activity of butlerius nematodes and nematophagous fungi against haemonchus contortus infective larvae.	the purpose of this study was to evaluate the predatory activity of the nematode butlerius spp. and fungal isolates of duddingtonia flagrans, clonostachys rosea, arthrobotrys musiformis and trichoderma esau against h. contortus infective larvae (l3) in grass pots. forty-eight plastic gardening pots containing 140 g of sterile soil were used. panicum spp. grass seeds (200 mg) were sown into each pot and individually watered with 10 ml of tap water. twelve days after seeding, the pots were randoml ...	2017	28146155
native fungi as metal remediators: silver myco-accumulation from metal contaminated waste-rock dumps (libiola mine, italy).	metal contamination constitutes a major source of pollution globally. many recent studies emphasized the need to develop cheap and green technologies for the remediation or reclamation of environmental matrices contaminated by heavy metals. in this context, fungi are versatile organisms that can be exploited for bioremediation activities. in our work, we tested silver (ag) bioaccumulation capabilities of three microfungal strains (aspergillus alliaceus thom & church, trichoderma harzianum rifai, ...	2017	28121268
transformation of the endochitinase gene chi67-1 in clonostachys rosea 67-1 increases its biocontrol activity against sclerotinia sclerotiorum.	clonostachys rosea is a promising biocontrol fungus active against various plant fungal pathogens. in this study, the endochitinase-encoding gene chi67-1, the expression of which is sharply upregulated in c. rosea 67-1 when induced by sclerotia, was transformed into the original isolate by protoplast transformation, and transformants were screened against sclerotinia rot of soybean. the transformation efficiency was approximately 50 transformants per 1 × 10(7) protoplasts, and 68 stably heritabl ...	2017	28050842