Publications

immunomodulatory effects of bacteroides products on in vitro human lymphocyte functions.	bacteroides spp. have been implicated in the pathogenesis of several diseases, including periodontal diseases. in this study sonic extracts of 6 bacteroides spp. were examined for their abilities to alter human lymphocyte function. we found that soluble extracts from bacteroides intermedius, bacteroides endodontalis, bacteroides asaccharolyticus, bacteroides melaninogenicus, and to a lesser degree bacteroides loescheii, caused dose-dependent inhibition of human lymphocyte responsiveness to both ...	1989	2628864
[microbiological changes in subgingival flora after treatment with amoxicillin-clavulanic acid].	the association amoxicillin-clavulanic acid can be employed as an alternative to the usual antibiotic therapy of periodontitis. the purpose of this study was to determine subgingival microbial changes in 33 patients with periodontitis after using amoxicillin (500 mgrs.-t.i.d.) and clavulanic acid (125 mgrs.-t.i.d.) for 5 days. it resulted clinically in a decrease of both gingival index and plaque index (not significant) and microbiologically in absence of the main bacterial pathogens found pretr ...	1989	2638817
surface appendages, hemagglutination, and adherence to human epithelial cells of bacteroides intermedius.	four types of morphologically distinct surface appendages were found on oral strains of bacteroides intermedius. these appendages, designated as a, b, c, and d, were different in size, with diameters of 1-2 nm, 12 nm, 8 nm, and 5 nm, respectively. twenty different strains were examined by electron microscopy and 5 strains, 5, 17, 27, 113, and 25611, were selected to be representative of the different appendages encountered. type a appendages were thin filaments peritrichously arranged on the cel ...	1989	2640314
the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis, bacteroides intermedius and spirochaetes in the subgingival microflora in relation to the early onset of periodontitis in a group of adolescents.	subgingival samples were obtained from mesial and distal surfaces of first molars in 100 subjects aged 14 years at baseline. sites were monitored at 6-monthly intervals for clinical loss of attachment. six subjects showed greater than 1 mm loss of attachment at one site 18-24 months later. further samples were obtained from these sites together with a comparison sample from the contralateral site and equivalent sites in a matched control subject. actinobacillus actinomycetemcomitans was more fre ...	1989	2640319
clinical, microbiological and immunological studies of post-juvenile periodontitis.	the present study includes the clinical, microbiological and immunological examinations of 2 patients with post-juvenile periodontitis. bacteroides intermedius was the predominant isolate from periodontal pockets with post-localized juvenile periodontitis. bacteroides gingivalis, bacteroides forsythus and actinobacillus actinomycetemcomitans were detected in samples from periodontal pockets with post-generalized juvenile periodontitis. igg antibody levels to b. gingivalis were significantly high ...	1989	2640920
hormonal influences on gingival tissue: relationship to periodontal disease.	it is the purpose of this review to survey the influence of corticosteroids, androgens, oestrogens and progesterone on gingival tissues and to show the relationship of such influences to periodontal disease. the clinical changes seen in plaque-induced gingivitis are accentuated by circulating levels of the above hormones via mechanisms such as partial immune suppression, increased fluid exudation, stimulation of bone resorption and stimulation of fibroblast synthetic activity. high counts of bac ...	1989	2654195
a comparison of fibronectinolytic activities from several oral bacteria.	fibronectinolytic activity from two gram-positive microorganisms (streptococcus mutans and bacterionema matruchotii), and from three gram-negative oral bacteria (bacteroides intermedius, bacteroides gingivalis and haemophilus actinomycetemcomitans) were compared. 125i-labelled human plasma fibronectin (fn) was incubated either either with bacterial extracts or with concentrated culture medium samples and the patterns of fn-degradation products were determined by sds-page. results to date have sh ...	1989	2693452
[microbiological and serological investigation of periodontal disease activity].	if disease activity in periodontal disease can be determined, the therapeutic measures of periodontitis may become diverse and different from patient to patient. the purpose of the present study was to evaluate the usefulness of microbiological and serological examination of periodontopathic bacteria in detecting destructive periodontal disease activity. one hundred and forty-eight sites in 52 subjects with moderate to severe periodontitis were studied clinically. clinical parameters included pl ...	1989	2700196
[microbiological evaluation of initial preparation for adult periodontitis patients].	the microbial flora from 46 adult periodontitis lesions of 23 patients and 18 sites in 9 healthy persons were examined and levels of serum igg antibody to gram negative periodontal disease-associated bacteria were measured by enzyme-linked immunosorbent assay. plaque samples and serum samples were taken 40-50 days after initial preparation consisting of scaling and root planing. to evaluate the effects of the therapy on 10 patients with adult periodontitis, changes in clinical parameters were co ...	1989	2700354
[microbial flora of periodontitis and monitoring of the effect of initial preparation by immunofluorescence microscopy].	the effect of initial preparation in adult periodontitis was evaluated by changes in clinical parameters and immunofluorescence microscopic counts of periodontal disease associated bacteria. subgingival plaque samples were taken with sterilized paper points from 10 sites of 5 periodontally healthy persons and 44 sites of 23 adult periodontitis patients. twenty-one diseased sites were periodically examined after plaque control and scaling, root planing. the direct immunofluorescence technique was ...	1989	2700361
neuraminidase-enhanced attachment of bacteroides intermedius to human erythrocytes and buccal epithelial cells.	bacteroides intermedius strains strongly agglutinated only neuraminidase-treated erythrocytes. the neuraminidase-dependent hemagglutinating activity of b. intermedius was abolished by heating or treating with protease. the adherence of these microorganisms to human buccal epithelial cells was enhanced by neuraminidase pretreatment of the cells (p less than 0.01).	1989	2707865
periodontal condition and microbiology of healthy and diseased periodontal pockets in type 1 diabetes mellitus patients.	on the basis of glycosylated hemoglobin (hba1c) values, 22 type 1 (insulin-dependent) diabetic adults were grouped into patients with near normal (hba1c less than or equal to 7.7%) and poor (hba1c greater than or equal to 9.9%) metabolic control. a total of 44 subgingival sites were examined for actinobacillus actinomycetemcomitans, black-pigmented bacteroides species and capnocytophaga species. no significant difference could be demonstrated between patients in the 2 test groups with regard to ...	1989	2723104
taxonomy, virulence and epidemiology of black-pigmented bacteroides species in relation to oral infections.	black-pigmented bacteroides species are recognized as suspected pathogens of oral infections. developments in the taxonomy of this group include description of a new asaccharolytic species, bacteroides salivosus, and proposal for the reclassification of the asaccharolytic species into a separate genus, porphyromonas. studies on the pathogenicity and virulence of black-pigmented bacteroides species have identified bacteroides gingivalis as the most virulent species. b. gingivalis and bacteroides ...	1989	2737764
activity of amine-stannous fluoride combination and chlorhexidine against some aerobic and anaerobic oral bacteria.	the in vitro susceptibility of 128 bacterial strains was tested to amine fluoride-stannous fluoride (amf + snf) and chlorhexidine (chx) solutions. actinobacillus actinomycetemcomitans, streptococcus mutans, and bacteroides intermedius were among the species investigated. the 50% and 90% minimal inhibitory concentrations (mics) were assessed by an agar dilution method. the mic ranges for a. actinomycetemcomitans were 2-32 micrograms/ml for chx and 0.25-64 micrograms/ml for amf + snf. the respecti ...	1989	2762015
growth and metabolic properties of bacteroides intermedius in anaerobic continuous culture.	two strains of bacteroides intermedius bh20/30 and bh18/23, have been grown in anaerobic continuous culture under various conditions for periods up to 54 days. strain bh20/30 grew over a relatively wide ph range from 5-8 with a maximum at ph 7.0 at a dilution rate (d) of 0.1 h-1 with a glucose limitation, while strain bh18/23 had an optimum between 5.8 and 7.3 and would not grow above and below this range. the maximum growth rate (mu max) for the latter strain was shown to be 0.23 h-1, or a doub ...	1989	2762020
the formation of hydrogen sulfide and methyl mercaptan by oral bacteria.	the capacity to form volatile sulfur compounds was tested in bacteria isolated from subgingival microbiotas and in a representative number of reference strains. a majority of the 75 tested oral bacterial species and 7 unnamed bacterial taxa formed significant amounts of hydrogen sulfide from l-cysteine. the most active bacteria were found in the genera peptostreptococcus, eubacterium, selenomonas, centipeda, bacteroides and fusobacterium. methyl mercaptan from l-methionine was formed by some mem ...	1990	2082242
characterization and physical mapping of plasmids of black-pigmented bacteroides.	eight species of black-pigmented bacteroides (bpb) were examined for plasmid content. three classes of plasmids with molecular sizes of 16.0 kilobase (kb), 5.4 kb and 5.0 kb were found in 7 of 125 bacteroides intermedius strains. one strain each of porphyromonas (former bacteroides) asaccharolyticus and of bacteroides levii were also shown to have plasmids. however, no plasmids were detected in 90 strains of porphyromonas (bacteroides) gingivalis. the plasmids were purified and digested with res ...	1990	2082244
effect of a triclosan/copolymer/fluoride dentifrice on the oral microflora.	eighty-one human subjects completed a double-blind study which examined the effects of a 0.3% triclosan/2% gantrez copolymer/0.243% sodium fluoride dentifrice on the microflora of supragingival dental plaque. subjects were divided into an experimental group which performed normal oral hygiene with the triclosan/copolymer/fluoride dentifrice and a control group which also performed normal oral hygiene with the same dentifrice minus the triclosan/copolymer. at baseline, 10 weeks, and 28 weeks, sup ...	1990	2083042
an in vitro method to study the adherence of bacteria to saliva-treated tooth enamel sections.	an in vitro bacterial adherence assay which employed human tooth enamel sections precoated with saliva and an epifluorescent staining technique with acridine orange was developed. the assay was used to study the adherence properties of fresh and type strains of the following oral bacterial species: bacteroides gingivalis, bacteroides intermedius, capnocytophaga species, haemophilus aphrophilus, actinobacillus actinomycetemcomitans, peptostreptococcus species, veillonella species, actinomyces isr ...	1990	2087341
the incidence of periodontopathic microorganisms in young children.	thirty-six children in 4 cohorts of 0-1 week, 1-6 months, 1-2 years and 2-2 1/2 years of age were examined for the presence of black-pigmented bacteroides species and some other bacteria suspected of being involved in the subsequent development of periodontal destruction. none of the sought-after bacteria were detected in the first week of life. bacteroides intermedius and bacteroides melaninogenicus were detected as early as 1 month after birth. both of these bacteria were detected in 16-37% of ...	1990	2087345
role of cryptic receptors (cryptitopes) in bacterial adhesion to oral surfaces.	progress in characterizing the receptors that promote bacterial attachment to teeth and oral epithelial cells has suggested that hidden molecular segments may frequently be involved. such cryptic receptors, referred to as 'cryptitopes', may become exposed by several mechanisms. hidden segments of salivary acidic proline-rich proteins evidently become exposed when the molecules undergo a conformational change as they adsorb to apatitic mineral. adhesins of actinomyces viscosus and certain other p ...	1990	2088213
bactericidal effects of human neutrophils and sera on selected endodontic pathogenic bacteria in an anaerobic environment.	phagocytosis by normal human neutrophils and the bactericidal activity of normal human pooled serum were measured under anaerobic conditions on six endodontic pathogenic bacterial species from the genera actinomyces, bacteroides, fusobacterium, peptostreptococcus and streptococcus. the results revealed that actinomyces viscosus was not killed either by human neutrophils or by 20 per cent serum; peptostreptococcus anaerobius was not killed by either 20 per cent or 95 per cent serum; and bacteroid ...	1990	2098335
microbiological features of stable osseointegrated implants used as abutments for overdentures.	the microflora associated with osseointegrated implants used as abutments for overdentures was investigated in 18 edentulous patients, 2 years after implantation. 52.8% of the organisms cultured were facultatively anaerobic cocci and 17.4% were facultatively anaerobic rods, while gram-negative anaerobic rods accounted for only 7.3%. fusobacterium sp. and bacteroides intermedius were both found in 8.8% of the samples. b. gingivalis and spirochetes were not found. repeated microbiological and clin ...	1990	2099207
studies of the subgingival microflora in patients with acquired immunodeficiency syndrome.	two unique forms of periodontal disease, hiv-gingivitis and hiv-periodontitis, have been described in patients with acquired immunodeficiency syndrome (aids). in order to determine the bacterial species associated with periodontitis in aids patients, the predominant cultivable microflora was examined in 21 subgingival plaque samples from 11 aids patients with periodontitis. the presence of putative periodontal pathogens including actinobacillus actinomycetemcomitans, bacteroides intermedius, por ...	1990	2123926
monoclonal antibody-coated latex agglutination assay for identification of actinobacillus actinomycetemcomitans.	three monoclonal antibodies (mabs) to lipopolysaccharide of actinobacillus actinomycetemcomitans strain y4 (serotype b) and eight mabs to a serotype b-specific polysaccharide antigen of strain y4 were obtained. latex particles sensitized with an mab to the y4 lipopolysaccharide produced a positive agglutination with whole cells of all three serotypes of a. actinomycetemcomitans, but not with haemophilus aphrophilus, haemophilus paraphrophilus, haemophilus influenzae, porphyromonas (bacteroides) ...	1990	2124492
in vitro survival of bacteroides intermedius in five transport media, alone or in the presence of streptococcus sanguis.	the survival rate of bacteroides intermedius was first tested in monoculture, and streptococcus sanguis was then added in 5 different transport media; 2 nonnutritious media, the viability-preserving medium of the university of göteborg no. iv (vmg iv), reduced transport fluid (rtf), and 3 nutritious media, thioglycolate medium (tg), peptone yeast extract medium (py) and py medium with 1% glucose (pyg). all manipulations were carried out in an anaerobic chamber. after a given transport time (6 or ...	1990	2129136
demonstration of bacteroides intermedius in periapical tissue using indirect immunofluorescence microscopy.	the presence of bacteria in periapical lesions of teeth with necrotic pulp has been demonstrated by microbiological sampling of periapical lesions during endodontic surgery. the purpose of this study was to confirm the presence of bacteroides intermedius in a periapical granuloma using an indirect immunofluorescence technique. histologic sections of a periapical granuloma were incubated with a rabbit antiserum to b. intermedius. after incubation with secondary antibody (fluorescein-conjugated go ...	1990	2133307
[mitogenic activity of lipopolysaccharide from bacteroides intermedius against c3h/hej mice spleen cells].	bacteroides intermedius is one of the periodontal disease generative bacteria. lipopolysaccharide preparation obtained from b. intermedius atcc 25611 and atcc 33563 strains was investigated as to limulus activity observed with pyrodic (endotoxin specific) reagent, and mitogenic activity in both normal and tolerant mice induced by escherichia coli. limulus activities of all lps's were nearly similar to that of e. coli. lps's preparation from b. intermedius were found to be strongly mitogenic for ...	1990	2134944
[changes of factors on disease activity in advancing periodontitis].	two test teeth, anteriors with greater than or equal to 6 mm deep periodontal pockets from each of 10 patients with advancing periodontitis were included in this study. the clinical signs of advancing periodontitis, generalized moderate to deep pockets and to severe loss of alveolar bone, were observed in young adult. there have been several reports on factors, which reflect the conversion clinically from infection by highly pathogenic plaque bacteria to a from of periodontitis displaying relati ...	1990	2135609
prepubertal periodontitis: a review of diagnostic criteria, pathogenesis, and differential diagnosis.	this paper reviews the literature on prepubertal periodontitis (pp) according to the definition and the classification of pp by page. the generalized form of pp (g-pp) defined by page was identified as the oral manifestation of a systemic disease called leukocyte adhesion deficiency (lad) and thus the pathogenesis is known at the molecular level. on the other hand, the localized form of pp (l-pp) is a disease defined by clinical criteria and its pathogenesis is not known at the molecular level. ...	1990	2137170
selective modulation of bacterial attachment to oral epithelial cells by enzyme activities associated with poor oral hygiene.	the present investigation explored the hypothesis that elevated levels of certain enzymes in the gingival crevicular environment of individuals with poor oral hygiene and/or gingival inflammation may modify the surfaces of epithelial cells and thereby modulate the types of bacteria which attach and colonize. buccal epithelial cells treated with neuraminidase and certain proteases were used as a model for study. bacteria studied included streptococcus sanguis and streptococcus mitis which have be ...	1990	2141877
inhibition of protease activities of periodontopathic bacteria by extracts of plants used in kenya as chewing sticks (mswaki).	extracts from five plants used as chewing sticks, and tannic acid, gallic acid and methyl ester of gallic acid, were tested for their ability to inhibit proteolytic activities of three strains of bacteroides gingivalis, three strains of bacteroides intermedius and two strains of treponema denticola. aqueous extract from the plants rhus natalensis and euclea divinorum were the most inhibitory of those tested, inhibiting by 50% the proteolytic activity of the test organisms, at concentrations of u ...	1990	2142592
alterations in cell morphology and cytoskeletal proteins in gingival fibroblasts exposed to a bacteroides gingivalis extract.	a soluble sonic extract (sse) from bacteroides gingivalis caused a dose-dependent inhibition of gingival fibroblast growth, reduced cell attachment and altered cell morphology. most of its cytotoxic activity was destroyed by heating, indicating that the factor(s) was a protein rather than endotoxin. cells, grown in the presence of, or on, root surfaces pretreated with 100-200 micrograms sse/ml, partially retracted from the substratum and exhibited extensive surface blebbing and finger-like protr ...	1990	2148946
suppression of bactericidal activity of human polymorphonuclear leukocytes by bacteroides gingivalis.	the direct effects of the culture supernatant of oral microorganisms on the bactericidal activity of human polymorphonuclear leukocytes (pmns) were investigated. the bactericidal activity of pmns, which were preincubated with the supernatant of bacteroides gingivalis, bacteroides intermedius, bacteroides melaninogenicus or phosphate-buffered saline, was examined by counting the surviving bacteria. b. gingivalis-treated pmns were found to have a diminished ability for killing bacteria in the pres ...	1990	2153632
effect of ph on the growth and proteolytic activity of porphyromonas gingivalis and bacteroides intermedius.	the effect of ph on the growth and proteolytic activity of the type strain and fresh isolates of porphyromonas gingivalis and bacteroides intermedius was investigated. b. intermedius strains grew with and without glucose at a ph as low as 5.0. these bacteria grew almost as well as streptococcus mutans at ph 5.0 and better than actinomyces viscosus at ph 5.5 and 5.0. some b. intermedius strains raised the culture ph when grown at a low ph without glucose. in contrast, p. gingivalis strains grew o ...	1990	2191980
bacteroides lipopolysaccharides (lps) induce anaphylactoid and lethal reactions in lps-responsive and -nonresponsive mice primed with muramyl dipeptide.	lipopolysaccharides (lps) (100 micrograms/mouse) from bacteroides gingivalis elicited anaphylactoid reactions in n-acetylmuramyl-l-alanyl-d-isoglutamine (mdp) (100 micrograms)-primed c3h/hen mice 0-48 h after mdp injection. the reaction resulted in death within 1 h when the lps was injected 2-10 h after the mdp injection. lps prepared from other bacteroides and salmonella species but not from others, including escherichia coli, could induce the reaction. lps-nonresponsive c3h/hej and c57bl/6 and ...	1990	2197336
application of monoclonal antibodies to the detection of black-pigmented bacteroides spp. in subgingival plaques by immunoslot blot assay.	the aim of the present study was to assess the application of monoclonal antibodies to the detection of black-pigmented bacteroides spp. in subgingival plaques by immunoslot blot assay. subgingival plaque samples from adult periodontal patients were examined by immunoslot blot assay with monoclonal antibodies that specifically recognize bacteroides gingivalis, bacteroides intermedius serogroups i and ii, and bacteroides melaninogenicus. the assay can detect specifically these bacteroides spp. in ...	1990	2229348
biochemical and serological characterization of bacteroides intermedius strains isolated from the deep periodontal pocket.	fifty-one fluorescence-positive black-pigmented bacteroides strains obtained from 51 patients with deep periodontal pockets (greater than 6 mm) were identified and characterized. fifty of these strains were presumptively identified as bacteroides intermedius according to the indole reaction. this was confirmed by further biochemical characterization. the 50 strains from diseased sites were then compared with 16 b. intermedius strains isolated from periodontally healthy individuals with no signs ...	1990	2229351
study of the antigenic relationships between strains of bacteroides, intermedius, b. melaninogenicus, b. corporis, and b. denticola revealed by immunoblotting with rabbit antisera.	antigen profiles of saccharolytic oral black-pigmented bacteroides have been developed by western blotting. visual comparisons indicated extensive cross-reactions between b. intermedius, b. melaninogenicus, b. denticola, and b. corporis. porphyromonas gingivalis, p. asaccharolyticus, and b. buccae showed less cross-reaction. quantitation of antigenic similarity was made from densitometric scans. calculation of the jackard coefficient gave results of 33-72% similarity among the saccharolytic pigm ...	1990	2249172
actinobacillus actinomycetemcomitans and bacteroides intermedius in human periodontitis: age relationship and mutual association.	this study examined age relationships and mutual interrelationships between cultivable actinobacillus actinomycetemcomitans and bacteroides intermedius in 1624 periodontitis patients, 15 to 89 years of age. each subject contributed a pooled subgingival sample, obtained from 3 deep periodontal pockets with paper points. a. actinomycetemcomitans occurred with higher prevalence (74%) and mean recovery (7% in culture-positive patients) in patients less than 25 years old than in adult and geriatric p ...	1990	2250080
cytotoxic and immunostimulatory effects of bacteroides cell products.	the etiologic role of bacteroides in both periodontal and periapical infections has been well documented, with current interest focusing on the specific pathogenic mechanisms involved. the effects of cell fractions derived from bacteroides gingivalis (bg), bacteroides intermedius (bi), and bacteroides asaccharolyticus (ba) have been studied in vitro through: an assessment of the direct cytotoxic effects on human gingival fibroblasts using a tetrazolium dye reduction assay, an evaluation of murin ...	1990	2250226
the effect of clindamycin on the microbiota associated with refractory periodontitis.	the purpose of this investigation was to determine the effect of clindamycin hydrochloride, as an adjunct to scaling, on the microbiota associated with refractory periodontitis and to elucidate the probable causative bacteria associated with the disease. microbial samples were collected from a subset of 9 patients with severe adult periodontitis who had not responded to conventional treatment modalities including the use of tetracycline and other antibiotics. microbial samples were collected fro ...	1990	2254836
[a comparative evaluation of oral and parenteral cephalosporins in inhibiting the growth of bacteria pathogenic to the periodontium].	a study was carried out in vitro to assess the inhibitory activity on the growth of bacteroides gingivalis and bacteroides intermedius using two oral cephalosporins (cephalexin and cephradine) in comparison to two parenteral cephalosporins (cefotaxime and ceftriaxon) and the classic tetracycline hcl. the results of the study show that the inhibitory activity on the growth of the two periodontopathogenic bacteria produced by the oral cephalosporins is comparable to that of other antibiotics studi ...	1990	2287333
detection of conjugal transfer systems in oral, black-pigmented bacteroides spp.	oral, black-pigmented bacteroides spp. are important pathogens in oral anaerobic infections and dental disease. we detected conjugation systems in isolates of bacteroides denticola and bacteroides intermedius that transferred tetracycline resistance (tetr) and penicillin resistance to bacteroides buccae and to bacteroides fragilis, an intestinal bacteroides species. a cloned tetr gene from b. fragilis hybridized to the transferable tetr locus in the oral strains, indicating that genetic exchange ...	1990	2294095
suspected periodontopathogens in erupting third molar sites of periodontally healthy individuals.	29 periodontally healthy subjects (11 female and 18 male) with a mean age of 24 years (range 19 to 38 years) and with partially erupted lower third molars participated in this study. 18 subjects demonstrated no signs or symptoms of acute inflammation and were without pain (group a). 5 subjects showed redness of the pericoronal tissues and experienced pain upon palpation (group b). 6 subjects suffered from acute pain and exhibited formation of pus (group c). microbiological samples were taken fro ...	1990	2295708
growth stimulation of treponema denticola by periodontal microorganisms.	previous experiments have indicated that enrichment of subgingival plaque in human serum can lead to the accumulation of treponema denticola. t. denticola depends on bacterial interactions for its growth in serum. aim of the present study was to identify specific microorganisms involved in the growth stimulation of t. denticola. to this end, strains isolated from previous plaque enrichment cultures were tested for growth stimulation in co-cultures with t. denticola. in addition, growth of t. den ...	1990	2321929
assessing periodontal pathogens in children with varying levels of oral hygiene.	this study investigated the presence of organisms actinobacillus actinomycetecomitans (a.a.), bacteroides intermedius (b.i.), and bacteroides gingivalis (b.g.), believed to be associated with periodontal disease etiology in children. the study included fifty children, aged six to fourteen years, selected from patients seeking routine dental care at children's hospital national medical center. a modified plaque index (mpi) denoting the clinical presence and severity of the periodontal disease was ...	1990	2345212
[the in vitro sensitivity of bacteroides gingivalis and bacteroides intermedius to different classes of antibiotic chemical molecules].	a study was carried out to assess the in vitro sensitivity of bacteroides gingivalis and bacteroides intermedius to various classes of chemio-antibiotic molecules. the activities of two cephalosporins (cefuroxime and cefotaxime), which have never previously been used for periodontal therapy, were also evaluated. results showed that the cephalosporins possessed an equal or greater inhibitory activity against the bacteria studied than traditional tetracyclines, and a greater inhibitory activity th ...	1990	2381396
antimicrobial effect of chlorhexidine in a controlled release delivery system.	chlorhexidine is widely used as a mouth rinse in the prevention and treatment of periodontal diseases and dental caries. the purpose of the present study was to evaluate the in vitro antimicrobial effect of chlorhexidine in a controlled release delivery system. the controlled release dispenser comprised a polymeric inner core matrix containing the medicament with an outer vinyl membrane controlling the drug release. the effect on the following bacteria was studied: actinobacillus actinomycetemco ...	1990	2390965
clinical and microbiologic findings that may contribute to dental implant failure.	clinical and dna probe analysis were used to evaluate 36 failing implant sites in 13 patients. failing implants showed evidence of increased mobility and a high incidence of peri-implant radiolucencies in radiographs. the probing depth was greater than 6 mm in 58% of the sites measured. moderate levels of actinobacillus actinomycetemcomitans, bacteroides intermedius, and bacteroides gingivalis were detected with dna probe analysis.	1990	2391137
occurrence of bacteroides gingivalis, bacteroides intermedius and actinobacillus actinomycetemcomitans in severe periodontitis in relation to age and treatment history.	a total of 242 subjects including 138 untreated severe periodontitis patients and 104 patients with refractory periodontal disease, previously treated for severe periodontitis, were examined for the occurrence of actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. pooled subgingival samples of representative periodontal lesions were used for anaerobic cultivation on blood agar and for the enumeration of a. actinomycetemcomitans on selective tsbv medium. 97% ...	1990	2398137
bacteroides gingivalis and bacteroides intermedius recognize different sites on human fibrinogen.	bacteroides (porphyromonas) gingivalis and bacteroides (porphyromonas) intermedius have been implicated in the etiology of human periodontal diseases. these organisms are able to bind and degrade human fibrinogen, and these interactions may play a role in the pathogenesis of periodontal disease. in attempts to map the bacterial binding sites along the fibrinogen molecule, we have found that strains of b. gingivalis and b. intermedius, respectively, recognize spatially distant and distinct sites ...	1990	2404954
reproducibility of microbiological samples from periodontal pockets.	duplicate microbiological samples, were taken 1 week apart using the paper point technique from a total of 112 untreated periodontal pockets greater than 6 mm deep in 16 adult periodontal patients. duplicate samples were also obtained from these sites 6 months following a therapy of oral hygiene instruction and supra- and subgingival debridement. the reproducibility of the total viable counts and the reproducibility of the proportions of various groups or species of microorganisms were studied f ...	1990	2406295
stimulation of lymphocytes in vitro by bacteroides intermedius and bacteroides (porphyromonas) gingivalis sonicates.	the present study was designed to assess whether the in vitro stimulation of lymphocytes by sonicates of bacteroides intermedius and bacteroides (porphyromonas) gingivalis is antigen specific or non-specific. in addition, the role of t and b lymphocytes in these responses was assessed. peripheral blood lymphocytes obtained from healthy volunteers were cultured in the presence of these bacterial preparations and the proliferative response was measured. in similar experiments the response of umbil ...	1990	1691286
the relationship of serum igg antibody titers to periodontal pathogens to indicators of the host response in crevicular fluid.	in this study; the relationship of indicators of the local host response in gingival crevicular fluid (gcf) to the serum antibody titer to periodontal pathogens was examined. 15 patients with chronic adult periodontitis were studied. gcf was collected and analyzed for the total amount of igg, igm, the lysosomal enzyme b-glucuronidase (bg) and alpha-2-macroglobulin (alpha 2m). at the same examination, serum from these patients was collected, and enzyme-linked immunosorbent assays used to determin ...	1990	1696949
[distribution of enzymatically pathogenic bacteria from periodontal pocket in advancing periodontitis].	production of 9 enzymatic activities of 527 strains freshly isolated from periodontal pockets in advancing periodontitis were investigated. of these isolates, two strains showed lecithinase activity on egg yolk agar plate. collagenase, plasmin and lipase were produced by 28 strains, 26 strains and 22 strains, respectively. two lecithinase-producing strains were identified as bacteroides intermedius. nineteen strains of b. intermedius and 1 strain of fusobacterium species produced lipase on egg y ...	1990	1966847
microbiological study of hiv-related periodontitis.	the subgingival microbiota in 14 persons with hiv-periodontitis was examined. subgingival plaque samples were collected with paper points, transported in vmga iii, and plated on anaerobic enriched brucella blood agar and various selective media. hiv-periodontitis sites revealed actinobacillus actinomycetemcomitans, wolinella recta, peptostreptococcus micros, and bacteroides intermedius, each averaging 7% to 16% of the cultivable subgingival flora in positive patients. high levels of spirochetes ...	1991	2002434
effects of chlorhexidine on proteolytic and glycosidic enzyme activities of dental plaque bacteria.	chlorhexidine was tested for its ability to inhibit a wide range of glycosidic and proteolytic enzyme activities produced by treponema denticola, porphyromonas gingivalis, bacteroides intermedius, actinobacillus actinomycemcomitans, capnocytophaga sputigena, capnocytophaga gingivalis, capnocytophaga orchracea, capnocytophaga sp., actinomyces viscosus, streptococcus mitior, streptococcus mutans, streptococcus sobrinus, streptococcus mitis, streptococcus anginosus, streptococcus oralis and strepto ...	1991	2005230
a randomized, placebo-controlled trial of doxycycline: effect on the microflora of recurrent periodontitis lesions in high risk patients.	twenty-seven patients with a recent history of periodontal abscesses and/or loss of gingival attachment level (gal) despite active periodontal therapy were enrolled in a double-blind, randomized, placebo-controlled trial. clinical measurements and subgingival scaling were performed every 2 months. when a site exhibited greater than or equal to 2 mm loss of gal or a periodontal abscess, patients were administered either doxycycline at a dosage of 200 mg to start and 100 mg per day for 3 weeks, or ...	1991	2027071
immune modulation of prevotella intermedia colonization in squirrel monkeys.	colonization of the gingival crevice by black-pigmented porphyromonas or prevotella spp. (bp/p), including porphyromonas gingivalis (formerly bacteroides gingivalis) and prevotella intermedia (formerly bacteroides intermedius), is thought to be an important ecological event which may result in the destruction of connective tissues supporting the teeth. theoretically, periodontal diseases could be prevented if these or other periodontal pathogenic microorganisms did not colonize the subgingival a ...	1991	2037354
evaluation of acrylic strips containing amoxycillin with clavulanic acid for local drug delivery.	the in vitro release of amoxycillin with clavulanic acid from acrylic strips at initial concentrations of 30, 40 and 50 per cent w/w was monitored using a double-beam ultraviolet spectrophotometer and compared with release of tetracycline hydrochloride. highest levels of the antibacterial agents were released during the first 24 h period. therapeutic levels of the drugs continued to be released during the subsequent 9 day period and were shown to be biologically active. furthermore, for amoxycil ...	1991	2050896
amoxycillin with clavulanic acid and tetracycline in periodontal therapy.	the effects of tetracycline and amoxycillin with clavulanic acid on the clinical parameters and subgingival flora of eight patients with rapidly progressive periodontitis was assessed. subjects received either tetracycline 250 mg four times daily or amoxycillin 250 mg with clavulanic acid 125 mg three times daily for a period of 2 weeks together with subgingival scaling and root planning. both treatment regimens produced significant reductions in bleeding on probing and probing pocket depths whi ...	1991	2050897
development of a rapid qualitative assay for determining elevated antibody levels to periodontopathic organisms.	to allow more widespread use of systemic antibody analysis in clinical settings, a rapid test for determining elevated antibody to periodontitis-associated bacteria was developed. the technique utilizes dot-immunoblotting (dib) on nitrocellulose paper with whole formalinized actinobacillus actinomycetemcomitans, porphyromonas gingivalis, and prevotella intermedia. an elisa was used to compare igg antibody levels to these organisms in venous serum and peripheral capillary blood from 44 subjects. ...	1991	2072241
microflora and bacterial dna restriction enzyme analysis in young adults with periodontitis.	as a result of an epidemiological survey on the periodontal status of young adults, a school was found with a relatively high number of subjects showing loss of attachment (18 out of 87). in 10 of these subjects the microflora of one pocket and of the dorsum of the tongue was investigated with special reference to black-pigmented species and actinobacillus actinomycetemcomitans. prevotella intermedia was the most prevalent species, being found in 9 patients. a. actinomycetemcomitans was isolated ...	1991	1645404
variance in recovery of periodontitis-associated bacteria caused by sampling technique and laboratory processing.	the influence of sampling procedure and of laboratory processing on the recovery of bacteroides gingivalis, bacteroides intermedius, and actinobacillus antinomycetemcomitans from periodontitis sites was evaluated. thirty-three adult subjects with severe periodontitis participated in the study. in all, 462 samples from 81 sites were examined. the samples were taken using the paper point technique. the cultivations were performed by use of enriched brucella agar for determination of total colony-f ...	1991	1658714
systemic antibody responses to oral microorganisms in the cynomolgus monkey: development of methodology and longitudinal responses during ligature-induced disease.	systemic antibody responses to oral microorganisms were studied during ligature-induced periodontal disease in a non-human primate (nhp) model. methodology was developed using elisa techniques to assess total igg and igm levels in the serum from the nhp. in addition, an elisa was developed utilizing affinity-purified anti-human isotype reagents to detect nhp serum antibody responses to porphyromonas gingivalis, actinobacillus actinomycetemcomitans, prevotella intermedia and fusobacterium nucleat ...	1991	1665573
inhibitory effect of stannous fluoride and other commonly used antimicrobial agents on oral bacteria.	the purposes of this investigation were to evaluate and compare the antimicrobial effect of stannous fluoride (snf2) gel and other commonly used oral antimicrobial agents/products. the antibacterial inhibitory effect of the various agents was evaluated by their effect against oral plaque bacteria including strains of streptococcus sanguis, streptococcus sobrinus, actinomyces viscosus, actinobacillus actinomycetemcomitans, bacteroides gingivalis and bacteroides intermedius. a lawn of the specific ...	1991	1681003
human lactoferrin binding to porphyromonas gingivalis, prevotella intermedia and prevotella melaninogenica.	human isolates of porphyromonas gingivalis (n = 16), prevotella intermedia n = 82) and prevotella melaninogenica (n = 18) from diseased periodontal pockets were examined for interaction with human lactoferrin (hlf) in a standardized 125i-labeled protein binding assay. the highest hlf binding was found in p. intermedia strains, followed by p. gingivalis and p. melaninogenica. further characterization of the interaction was performed with 1 representative strain from each species. hlf binding to p ...	1991	1726544
[chronic conjunctivitis caused by oral anaerobic germs].	a patient with chronic, unilateral conjunctivitis is presented. the predominant bacterial flora consisted mainly of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. restriction endonuclease fingerprinting of genomic dna demonstrated that p. intermedia most probably originated from the oral cavity. in the oral cavity this species was recovered from the dorsum of the tongue, the tonsillar area and the periodontal pockets. topical treatment with various antibi ...	1991	1758517
fluorescence immunoassay for detecting periodontal bacterial pathogens in plaque.	a particle concentration fluorescence immunoassay has been modified into a bacterial concentration fluorescence immunoassay (bcfia) to rapidly detect periodontopathic bacteria in human plaque samples. the bcfia utilizes fluorescently tagged monoclonal antibodies (mabs) directed against the lipopolysaccharide of selected gram-negative plaque bacteria. microorganisms closely associated with periodontal disease that can be identified in plaque with the bcfia include porphyromonas gingivalis, bacter ...	1991	1761686
rapid presumptive identification of black-pigmented gram-negative anaerobic bacteria by using 4-methylumbelliferone derivatives.	a rapid method for presumptive identification of black-pigmented gram-negative anaerobic rods was developed. using filter paper spot tests for indole production, sialidase, alpha-glucosidase, beta-glucosidase, alpha-fucosidase, and trypsinlike enzyme activities, 100% of porphyromonas gingivalis, prevotella intermedia, and bacteroides levii and 89% of prevotella corporis isolates were correctly identified to the species level. porphyromonas asaccharolytica and porphyromonas endodontalis could not ...	1991	1774320
biological effects of veillonella parvula and bacteroides intermedius lipopolysaccharides.	a comparative study on the endotoxic effects of lipopolysaccharide (lps) from veillonella parvula atcc 10790 and from bacteroides intermedius bmh was performed using an in vivo approach in the c57bl/6 mouse. phenol-water extracted lps of such anaerobes was purified by ultracentrifugation and dnase/rnase digestion, and characterized by a metachromatic assay for endotoxins and by electrophoresis on sds-polyacrylamide gel and silver staining. mouse ld50 for v. parvula lps was 1.479 mg and for b. in ...	1991	1775087
[subgingival microflora in children of early childhood, school age and circumpuberty. the proportion and frequency of gram-negative bacteria in periodontally healthy and gingivitis groups].	the present study characterized the microbial profiles of the gingival sulci in children. subgingival samples from 36 gingivitis lesions of 18 patients and 36 sites in 18 healthy persons were examined. the tested individuals were divided into three stages according to physiological maturation i.e. early childhood, school age, circumpuberty. puberty was confirmed through examination of wrist radiographs. using continuous anaerobic techniques, samples were dispersed, diluted and then inoculated on ...	1991	1784868
[bacteriological examination of infections in the field of oral surgery].	etiology of bacterial infections in the field of oral surgery was studied. a total of 270 samples collected from patients with encapsulated abscess in their oral cavities was examined and bacteria were isolated from the 244 samples (90.4%). the following results were found; 1) organisms more than one from one sample were frequently isolated from cases with parodontitis, pericoronitis and gnathitis. isolation of anaerobic bacteria was common (54.2%). 2) streptococcus milleri and streptococcus san ...	1991	1791332
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.	to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...	1991	1800380
adherence to experimental pellicle of rough-type lipopolysaccharides from subgingival plaque bacteria.	the adherence of lipopolysaccharides (lpss) from periodontal disease-associated bacteria to saliva-coated hydroxyapatite (s-ha) and serum-coated ha beads was examined by chromogenic limulus activity (toxicolor test). phenol-water extracted lps preparations from bacteroides intermedius, fusobacterium nucleatum, actinobacillus actinomycetemcomitans, eikenella corrodens, and rough-type lps from escherichia coli adhered to s-ha and serum-coated beads and agglutinated human erythrocytes. the adhered ...	1991	1812466
antimicrobial activity of flurbiprofen and ibuprofen in vitro against six common periodontal pathogens.	nonsteroidal antiinflammatory drugs (nsaids) such as flurbiprofen and ibuprofen, have been shown to inhibit the inflammation and alveolar bone loss associated with chronic destructive periodontal disease. however, the direct effect of nsaids on the gingival crevice microflora has not been studied. the purpose of this investigation was to evaluate the antimicrobial activity of ibuprofen and flurbiprofen in vitro on six commonly isolated periodontal pathogens. the bacterial strains evaluated were ...	1991	1812907
production of trypsin and chymotrypsin inhibitors by oral bacterial isolates.	using an agar-skim milk gel method, over 300 oral bacterial isolates were tested for their ability to produce trypsin or chymotrypsin inhibitor. eleven bacterial strains, including seven isolates identified as bacteroides intermedius, were found to inhibit both proteolytic activities. the inhibitory factors of the 11 bacterial strains could be divided into two categories: i) heat-stable and dialysable, and ii) heat-labile and non-dialysable. the protease inhibitor activity of b. intermedius stra ...	1991	1832450
multicenter evaluation of tetracycline fiber therapy. iii. microbiological response.	in a multicenter study of the effects of tetracycline (tc) fiber therapy, subgingival plaque samples were tested for 6 probable periodontal pathogens by dna probe analysis. levels of actinobacillus actinomycetemcomitans, eikenella corrodens, fusobacterium nucleatum, porphyromonas (bacteroides) gingivalis, prevotella intermedia (bacteroides intermedius), and wolinella recta were quantitatively determined in samples taken at baseline, and immediately after tc fiber removal, control fiber removal, ...	1991	1832457
tetracycline fiber therapy monitored by dna probe and cultural methods.	oligonucleotide dna probe and selective cultural methods were compared in their ability to monitor 6 putative periodontal pathogens in a study evaluating local tetracycline fiber therapy. subgingival plaque was sampled from 4 sites in each of 20 subjects. samples were taken before and after therapy from sites assigned to the following test groups: tetracycline (tc) fiber, scaling and root planing, control fiber, and untreated. each sample was analyzed by both dna probe and cultural methods. tota ...	1991	1832458
cd4 to cd8 ratio and in vitro lymphoproliferative responses during experimental gingivitis in pregnancy and post-partum.	the absolute numbers and percentages of peripheral t, b, and nk cells were assessed in 7 women, both during the second trimester of pregnancy and 6 months post-partum. furthermore, the in vitro responses of peripheral blood lymphocytes (pbl) to several mitogens and a preparation of prevotella intermedia were compared in a period of experimentally-induced gingivitis during pregnancy and post-partum. clinically, the periodontal pocket bleeding index (ppbi) was found to be higher during pregnancy t ...	1991	1836497
incidence of periodontitis recurrence in treated patients with and without cultivable actinobacillus actinomycetemcomitans, prevotella intermedia, and porphyromonas gingivalis: a prospective study.	a total of 98 adults previously treated for moderate to advanced periodontitis and on a trimonthly recall schedule were screened for the presence of critical levels of actinobacillus actinomycetemcomitans, prevotella (bacteroides) intermedia, and porphyromonas (bacteroides) gingivalis. patients with at least 2 positive sites were placed in a positive group and patients without or with low levels of these bacteria in a negative group. during the 30-month study the incidence of disease recurrence ...	1991	1870068
the stability of outer-membrane protein and antigen profiles of a strain of bacteroides intermedius grown in continuous culture at different ph and growth rates.	the stability of the outer-membrane proteins and antigens of a strain of bacteroides intermedius (vpi 8944 group genotype ii) grown in continuous culture at varying ph and growth rates (d = 0.025-0.2 h-1, ph 6.0-7.3) has been measured. the membranes showed nine major proteins (greater than 67-19.55 kilodaltons) and six major antigens (65-28 kilodaltons). membrane proteins and antigens were stable under the conditions tested; the major proteins were detected in all membranes, and the antigen prof ...	1991	1878815
chronic conjunctivitis caused by oral anaerobes and effectively treated with systemic metronidazole plus amoxicillin.	in this study, we report on a case of refractory, unilateral anaerobic conjunctivitis. the predominant anaerobic flora consisted of prevotella intermedia (formerly bacteroides intermedius) and peptostreptococcus micros. by using the technique of restriction endonuclease fingerprinting of genomic dna, it was shown that the p. intermedia likely originated from the oral cavity. topically applied antibiotics had failed to suppress the infection in the past. successful treatment was achieved after sy ...	1991	1890173
the use of genomic dna fingerprinting in studies of the epidemiology of bacteria in periodontitis.	recent studies of microbial epidemiology emphasizing the genetic organization and distribution of organisms associated with orofacial infections have led to new insights into the possible origins of pathogenicity. studies into genetic heterogeneity, acquisition and transmission of these organisms have been markedly advanced by the utilization of the powerful technique of genomic dna fingerprinting. characteristic fingerprints for each bacterial isolate can be produced by cleavage of high molecul ...	1991	1890219
microbiology in the management of destructive periodontal disease.	this paper summarizes the rationale for the application of microbiology in the management of destructive periodontal diseases. the subgingival microbiota in patients with severe periodontitis is complex and contains high numbers of obligate anaerobic bacteria as well as facultative micro-organisms. it has become clear that major differences exist in the composition of the subgingival microflora. these differences are not only quantitative but also qualitative. difference in plaque composition is ...	1991	1890220
dental bacterial plaques. nature and role in periodontal disease.	antony van leeuwenhoek first described oral bacteria. however, not until almost 200 years later was the famous koch postulate introduced. since then, research has extensively been performed regarding the development and microbiology of dental plaques. in spite of the complexity of the developing flora of supragingival plaque, culture studies have shown a remarkably orderly succession of organisms. lately, the concept of microbial specificity in the etiology of periodontal diseases has been widel ...	1991	1890226
effects of immunization with porphyromonas gingivalis and prevotella intermedia on progression of ligature-induced periodontitis in the nonhuman primate macaca fascicularis.	the nonhuman primate (nhp) has proven to be a useful model of human periodontitis. this study describes the immunological characteristics of this model and the ability of active immunization to interfere with ecological changes in the microbiota and its associated disease symptoms. nhps were parenterally immunized with whole-cell antigens of porphyromonas gingivalis and prevotella intermedia. the immunization elicited an approximate 2-log increase in serum immunoglobulin g (igg), igm, and iga is ...	1991	1894349
prostaglandin e release from human monocytes treated with lipopolysaccharides isolated from bacteroides intermedius and salmonella typhimurium: potentiation by gamma interferon.	the purpose of this investigation was to examine gamma interferon potentiation of lipopolysaccharide (lps) responses in human monocytes by using phenol-water-extracted (unfractionated) and highly purified lps preparations isolated from bacteroides intermedius and salmonella typhimurium. phenol-water-extracted lps preparations from these bacteria were further purified by chromatography over sepharose-cl-4b. lps enrichment in pooled column fractions was assessed by sodium dodecyl sulfate-polyacryl ...	1991	1898900
identification, and susceptibility to seven antimicrobial agents, of 61 gram-negative anaerobic rods from periodontal pockets.	sixty-one cultures of gram-negative anaerobic rods were isolated from deep periodontal pockets of patients with rapidly progressive periodontitis. isolates were speciated as bacteroides gingivalis (18 isolates), bacteroides intermedius (8), bacteroides oris (1), bacteroides gracilis (17) and fusobacterium nucleatum (17). their susceptibilities, to seven antimicrobial agents, were determined in vitro using a plate dilution technique. amoxycillin and amoxycillin with clavulanic acid were active ag ...	1991	1901873
microbial aspects of failing osseointegrated dental implants in humans.	the subgingival microflora of 18 failing implants were examined for pathogenic periodontal microorganisms. peptostreptococcus micros was recovered from 6 failing implants, wolinella recta from 6, fusobacterium species from 5, candida albicans from 5, and bacteroides intermedius from 4. enteric rods or pseudomonads constituted a significant part of the microflora in 5 failing implants. actinobacillus actinomycetemcomitans, nonpigmented bacteroides species, capnocytophage species, and staphylococc ...	1991	1906537
isolation and purification of bacteriocin from prevotella intermedia (bacteroides intermedius).	thirty-three out of 46 strains of prevotella intermedia (bacteroides intermedius) isolated from gingival deposits of patients with adult periodontitis possessed the ability to inhibit growth of p. intermedia strain atcc 25611, and 6 of the 33 showed strong inhibitory activity. thirteen isolates did not have the ability to inhibit the growth of p. intermedia strain atcc 25611. an inhibition factor from one of the isolates, p. intermedia th14 which had strong inhibition, was purified and character ...	1991	1920011
immunosuppressive effects of prevotella intermedia on in vitro human lymphocyte activation.	in this study, we have assessed four strains of prevotella intermedia, isolated from periodontally involved lesions, for their ability to inhibit lymphocyte functions. all four strains were found to cause a dose-dependent inhibition of b- and t-cell proliferation in response to mitogens and antigens. this was reflected in altered dna, rna, and protein syntheses. furthermore, p. intermedia appeared to affect the early stages of cell activation. this was ascertained by kinetic analysis in which it ...	1991	1937818
detection and management of the high risk periodontal patient.	periodontal diseases and patients at risk for progression of disease can be diagnosed on the basis of age, previous episodes of periodontal disease and loss of attachment, as detected by periodontal probing and radiographic evaluation. for early onset periodontitis, family history may be important. microbiological evaluations are excellent determinants of disease and also appear to be probable predictors of 'at risk' patients. reliable tests, including chairside tests, are available for the thre ...	1991	1937850
survival of prevotella intermedia (bacteroides intermedius) in transport media.	five transport media were selected for testing, in vitro, the survival of a pure strain of prevotella intermedia (bacteroides intermedius) for 6, 24, and 72 hours. two were non-nutrient transport media (rtf and vmg iv). the three others (tg, py, pyg) were nutrient media. an increase in the transport time, and manipulations in an aerobic atmosphere compromised the survival of the bacteria. py ensured good survival of the bacteria. rtf seems to be the best medium for the transport of p. intermedia ...	1991	1939047
reactivity of igg from explant cultures of periapical lesions with implicated microorganisms.	the presence of igg in periapical inflammatory lesions suggests that immune responses participate in the disease process. the purpose of this investigation was to study the reactivity of igg from the supernatant fluids of explant cultures of periapical lesions with microorganisms implicated in infections of endodontic origin. ninety periapical lesions that had been contiguous with the apex of a root were removed and maintained in explant cultures. a dot-enzyme-linked immunosorbent assay (dot-eli ...	1991	1940739
dna probe detection of periodontal pathogens in hiv-associated periodontal lesions.	recent studies have shown that an atypical gingivitis and a rapidly progressive periodontal disease may be early-occurring opportunistic infections associated with human immunodeficiency virus (hiv) infection. this study examined the prevalence of selected periodontal pathogens associated with these hiv-related periodontal lesions. subgingival plaque samples were obtained from both hiv-seronegative and hiv-seropositive homosexual men and from presumably uninfected heterosexual men. dna probes we ...	1991	1945481
rapid antimicrobial resistance screening method for bacteroides intermedius.	the purpose of the study was to validate a rapid resistance screening (rrs) method for antimicrobial susceptibility testing of a selected periodontopathic microorganism using the standard broth dilution method as a control. twenty-five subgingival plaque samples from gingivitis or periodontitis sites were plated on trypticase soy agar supplemented with 5% rabbit blood with antibiotic discs (rrs method) and without (control). the antibiotics tested were: augmentin, clindamycin, erythromycin, metr ...	1991	1945485
dna probe detection of eikenella corrodens, wolinella recta and fusobacterium nucleatum in subgingival plaque.	this cross-sectional study used species-specific dna probes to examine subgingival plaque specimens for the presence of eikenella corrodens, wolinella recta, and fusobacterium nucleatum in adults with untreated periodontitis or gingivitis and in healthy controls. w. recta and f. nucleatum were more prevalent in diseased sites from the periodontitis group when compared with the controls (81% vs 22% and 83% vs 20% respectively). e. corrodens was detected in 62% of the control sites and 81% of the ...	1991	1945492
effects of propicillin on mixed continuous cultures of periodontal bacteria.	experiments were designed to test the antibiotic (1-phenoxypropyl)penicillin (propicillin) against a complex microflora of periodontal bacteria. this was accomplished by using a continuously growing mixed culture that was obtained by enrichment of periodontal plaque in human serum. peptostreptococcus species, prevotella intermedia, lactobacillus, catenaforme, and streptococcus species were dominant members of the enrichment culture. none of the strains isolated from the enrichment culture exhibi ...	1991	1952836