Publications

comparative proteomic analysis of differentially expressed proteins in the bombyx mori fat body during the microsporidia nosema bombycis infection.	nosema bombycis is an obligate intracellular parasite, which can cause pébrine disease. to investigate the effects of n. bombycis infection, 5th-instar silkworms were challenged with n. bombycis isolate cq1, and two-dimensional gel electrophoresis analysis was performed to analyze the differentially expressed proteins in infected and uninfected silkworm fat bodies 1, 2, 4, 6 and 8days post-infection (dpi). 46 differentially expressed proteins were identified at the 5 time points using maldi-tof/ ...	2017	28668257
divergence of a tandem duplication of manganese superoxide dismutase in nosema bombycis.	manganese superoxide dismutase (mnsod) is a key enzyme in the protection of cells from oxidative stress. a tandem duplication of the mnsod gene (nbmnsod1 and nbmnsod2) in the genome of nosema bombycis, a parasite of the silkworm bombyx mori, was previously identified. here, we compare the protein structures of nbmnsod1 and nbmnsod2 and characterize these two proteins in terms of cellular localization, timing of transcription, protein structure, and enzyme activity. despite a similarity in the pr ...	2017	28691191
functional characterization of an aquaporin from a microsporidium, nosema bombycis.	microsporidia are a diverse group of eukaryotic organisms, capable of causing parasitic infections in both vertebrates and invertebrates. during the germination process, there is an increase in the osmotic pressure of microsporidian spores. as part of this study, we cloned a homologous aquaporin gene in nosema bombycis, and named it nosema bombycis aquaporin (nbaqp). sequence analysis revealed that the nbaqp contains an open reading frame with a length of 750 bp and encodes a polypeptide of 249 ...	2017	28749993
morphological characterization and hsp70-, igs-based phylogenetic analysis of two microsporidian parasites isolated from antheraea pernyi.	two microsporidian isolates were extracted from single infected egg-laying tussah silk moth (antheraea pernyi) in liaoning province, china. the microsporidia were subsequently grown in silk moth larvae, isolated, and subjected to morphological characterization (by light and transmission electron microscopy) and phylogenetic analysis (based on conserved genes). one type of spore was long-axis-oval in shape, measuring 4.71 × 1.95 μm, and the other type was short-axis-oval, measuring 3.64 × 2.17 μm ...	2017	28111712
interaction between swp9 and polar tube proteins of the microsporidian nosema bombycis and function of swp9 as a scaffolding protein contribute to polar tube tethering to the spore wall.	all microsporidia possess a unique, highly specialized invasion mechanism that involves the polar tube and spore wall. the interaction between spore wall proteins (swps) and polar tube proteins (ptps) in the formation, arrangement, orderly orientation, and function of the polar tube and spore wall remains to be determined. this study was undertaken to examine the protein interactions of nosema bombycis swp7 (nbswp7), nbswp9, and ptps. coimmunoprecipitation, liquid chromatography-tandem mass spec ...	2017	28031263
bacterium-expressed dsrna downregulates microsporidia nosema bombycis gene expression.	the microsporidia nosema bombycis is the insect pathogen of pebrine disease severely destructive to sericulture production. here, we describe the use of escherichia coli ht115 strain (de3) to express double-strand rnas targeting the gene encoding adp/atp protein in n. bombycis. the results showed that dsrnas deferentially suppressed the gene expression during n. bombycis infection in the silkworm, and the effect waned gradually. our results, for the first time, provide a tool to utilize the dsrn ...	2017	27397809
comparative analysis of the proteins with tandem repeats from 8 microsporidia and characterization of a novel endospore wall protein colocalizing with polar tube from nosema bombycis.	as a common feature of eukaryotic proteins, tandem amino acid repeat has been studied extensively in both animal and plant proteins. here, a comparative analysis focusing on the proteins having tandem repeats was conducted in 8 microsporidia, including four mammal-infecting microsporidia (encephalitozoon cuniculi, encephalitozoon intestinalis, encephalitozoon hellem and encephalitozoon bieneusi) and four insect-infecting microsporidia (nosema apis, nosema ceranae, vavraia culicis and nosema bomb ...	2017	28321967