Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| a simplified method for cloning of short interfering rnas from brassica juncea infected with turnip mosaic potyvirus and turnip crinkle carmovirus. | rna silencing is a plant defense mechanism in which virus infected plants produce short interfering rnas (sirnas) derived from viral rna, that attack the virus at the post-transcriptional level. in a previous study on cymbidium ringspot tombusvirus (cymrsv) infection in nicotiana benthamiana, sirnas (determined by cloning and sequencing) predominantly originated from the sense (+) strand of the viral rna, suggesting that the majority of sirnas are produced through the direct cleavage of the viru ... | 2006 | 16815561 |
| introgression of a tombusvirus resistance locus from nicotiana edwardsonii var. columbia to n. clevelandii. | abstract a new variety of nicotiana, n. edwardsonii var. columbia, was evaluated for its capacity to serve as a new source for virus resistance genes. columbia was developed from a hybridization between n. glutinosa and n. clevelandii, the same parents used for the formation of the original n. edwardsonii. however, in contrast to the original n. edwardsonii, crosses between columbia and either of its parents are fertile. thus, the inheritance of virus resistance genes present in n. glutinosa cou ... | 2006 | 18944304 |
| contact dermatitis due to orchids (cymbidium and oncidium). | a 64-year-old male who cultivated orchids as a hobby, had noticed itchy erythematous lesions on both hands since 6 months earlier. his eruptions had gradually worsened and scales and fissures had appeared. although he was treated with topical corticosteroids by a doctor, his erythema showed no improvement. patch tests with the orchids he cultivated, fertilizers, antiseptics and insecticides showed positive reactions to the stems of cymbidium and oncidium orchids. a diagnosis of contact dermatiti ... | 2006 | 16556279 |
| a rudimentary labellar speculum in cymbidium lowianum (rchb.f.) rchb.f. and cymbidium devonianum paxton (orchidaceae). | the labellar 'hairs' of some cymbidium spp. are said to be thin-walled and to contain 'plasma', oil and sugars and it has long been speculated that they may function as food-hairs. however, the present authors' preliminary studies showed that certain atypical papillae may have a different role and, by reflecting light, function as a speculum. the purpose of the paper is to test this hypothesis. | 2006 | 16574692 |
| use of superparamagnetic beads for the isolation of a peptide with specificity to cymbidium mosaic virus. | a modified method for the rapid isolation of specific ligands to whole virus particles is described. biopanning against cymbidium mosaic virus was carried out with a commercial 12-mer random peptide display library. a solution phase panning method was devised using streptavidin-coated superparamagnetic beads. the solution based panning method was more efficient than conventional immobilized target panning when using whole viral particles of cymbidium mosaic virus as a target. enzyme-linked immun ... | 2006 | 16781785 |
| gigantol isolated from the whole plants of cymbidium goeringii inhibits the lps-induced inos and cox-2 expression via nf-kappab inactivation in raw 264.7 macrophages cells. | during our efforts to find bioactive natural products with anti-inflammatory activity, we isolated gigantol from the whole plants of cymbidium goeringii (orchidaceae) by activity-guided chromatographic fractionation. gigantol was found to have potent inhibitory effects on lps-induced nitric oxide (no) and prostaglandin e (2) (pge (2)) production in raw 264.7 cells. consistent with these findings, gigantol suppressed the expression of inducible nitric oxide synthase (inos) and cyclooxygenase-2 (c ... | 2006 | 16924582 |
| [in vitro flowering of cultures from a hybrid of cymbidium goeringii and c. hybridium]. | wild-type female spring orchid (cymbidium goeringii) was crossed with male cymbidium hybridium. over eight hundred protocorm clones were obtained from hybrid offsprings. among them, one protocorm clone was identified to differentiate visible floral buds two months after subculture in vitro (plate i). the protocorms and shoots derived from this clone were further used in studying the effects of abscisic acid (aba) and paclobutrazol (pp333) pretreatment as well as different concentrations of 6-ben ... | 2006 | 16775400 |
| orchids (cymbidium spp., oncidium, and phalaenopsis). | recent advances in genetic engineering have made the transformation and regeneration of plants into a powerful tool for orchid improvement. this chapter presents a simple and reproducible agrobacterium tumefaciens-mediated transformation protocol and molecular screening technique of transgenics for two orchid species, oncidium and phalaenopsis. the target tissues for gene transfer were protocorm-like bodies (plbs) derived from protocorms, into which constructed foreign genes were successfully in ... | 2006 | 17033075 |
| evidence that cymbidium mosaic virus (cymmv) isolates divide into two subgroups based on nucleotide diversity of coat protein and replicase genes. | the genetic diversity of cymbidium mosaic virus (cymmv, family flexiviridae) was assessed by analysing the nucleotide sequences of coat protein (cp) and partial rna-dependent rna polymerase (rdrp) genes. thirty cymmv sequences from vanilla isolates, obtained in this work by direct sequencing of rt-pcr products, were compared to the sequences from ornamental orchid isolates available in genbank. the cymmv population exhibited overall low genetic diversity (pi=0.054 and pi=0.053 for cp and rdrp ge ... | 2007 | 17216134 |
| carbohydrate-binding agents efficiently prevent dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (dc-sign)-directed hiv-1 transmission to t lymphocytes. | exposure of hiv-1 to dendritic cell-specific intercellular adhesion molecule-3-grabbing nonintegrin (dc-sign)-expressing b-lymphoblast raji cells (raji/dc-sign) but not to wild-type raji/0 cells results in the capture of hiv-1 particles to the cells as measured by the quantification of cell-associated p24 antigen. cocultivation of hiv-1-captured raji/dc-sign cells with uninfected cd4+ t lymphocyte c8166 cells results in abundant formation of syncytia within 36 h after cocultivation. short preexp ... | 2007 | 17056872 |
| structure of cymbidine a, a monomeric peptidoglycan-related compound with hypotensive and diuretic activities, isolated from a higher plant, cymbidium goeringii (orchidaceae). | the structure of a new monomeric peptidoglycan-related compound with hypotensive and diuretic activities, cymbidine a (1) isolated from the orchid cymbidium goeringii, was elucidated mainly by spectroscopic analysis. the structure of 1 was shown to involve four amino acids (d-alanin, meso-diaminopimelic acid, d-gultamic acid, and l-valine) and two amino sugars (n-acetylglucosamine and 1,6-anhydro-n-acetylmuramic acid). the sequence of the amino acids and amino sugars was determined by the analys ... | 2007 | 17473468 |
| antiviral activity of carbohydrate-binding agents against nidovirales in cell culture. | coronaviruses are important human and animal pathogens, the relevance of which increased due to the emergence of new human coronaviruses like sars-cov, hku1 and nl63. together with toroviruses, arteriviruses, and roniviruses the coronaviruses belong to the order nidovirales. so far antivirals are hardly available to combat infections with viruses of this order. therefore, various antiviral strategies to counter nidoviral infections are under evaluation. lectins, which bind to n-linked oligosacch ... | 2007 | 17560666 |
| strategies for functional validation of genes involved in reproductive stages of orchids. | plants in the largest family of angiosperms, orchidaceae, are diverse in both specialized pollination and ecological strategies and provide a rich source for investigating evolutionary relationships and developmental biology. however, studies in orchids have been hindered by several challenges that include low transformation efficiency and long regeneration time. to overcome such obstacles, we selected a symptomless cymbidium mosaic virus (cymmv) isolate for constructing virus-induced gene-silen ... | 2007 | 17189336 |
| agrobacterium-mediated transformation of protocorm-like bodies in cymbidium. | genetically transformed plants of cymbidium were regenerated after cocultivating protocorm-like bodies (plb) with agrobacterium tumefaciens strain eha101 (pig121hm) that harbored genes for beta-glucuronidase (gus), hygromycin phosphotransferase (hpt) and neomycin phosphotransferase ii (nptii). plb of three genotypes maintained in liquid new dogashima medium (ndm), were subjected to transformation experiments. the plb inoculated with agrobacterium produced secondary plb, 4 weeks after transfer on ... | 2007 | 17205333 |
| [molecular identification of cymbidium mosaic potexvirus and odontoglossum ringspot tobamovirus complex infected phalaenopsis and its pathological ultrastructural alteration]. | filamentous and rod-shaped virions, and aggregated crystals were observed in infected leaves of by negative staining and ultramicrotomy. histologic study synchronously showed typical crystal forms of the two virions: the crystals from filamentous particles congregated in strips, arrayed in multilayer and piled in certain angles or helix between layers; while the crystals from rod-shaped particles arrayed in parallel, angle-layer or helix. the two kinds of crystals both presented in parenchyma ce ... | 2007 | 17580669 |
| complete nucleotide sequence analysis of cymbidium mosaic virus indian isolate: further evidence for natural recombination among potexviruses. | the complete nucleotide sequence of an indian strain of cymbidium mosaic virus (cymmv)was determined and compared with other potexviruses. phylogenetic analyses on the basis of rna-dependent rna polymerase (rdrp), triple gene block protein and coat protein (cp) amino acid sequences revealed that cymmv is closely related to the narcissus mosaic virus (nmv), scallion virus x (svx), pepino mosaic virus (pepmv)and potato aucuba mosaic virus (pamv). different sets of primers were used for the amplifi ... | 2007 | 17762139 |
| silencing of the n family of resistance genes in nicotiana edwardsonii compromises the hypersensitive response to tombusviruses. | the nontarget effects associated with silencing of the n gene in nicotiana edwardsonii, an amphidiploid species derived from n. glutinosa and n. clevelandii, have been characterized in this study. the n protein confers resistance to tobacco mosaic virus (tmv), and is representative of a family of nucleotide-binding site leucine-rich repeat proteins present in n. glutinosa. previous studies have shown that silencing of the n gene or of other plant genes associated with n-mediated defenses abolish ... | 2007 | 17918628 |
| cymbidium ringspot virus defective interfering rna replication in yeast cells occurs on endoplasmic reticulum-derived membranes in the absence of peroxisomes. | the replication of cymbidium ringspot virus (cymrsv) defective interfering (di) rna in cells of the yeast saccharomyces cerevisiae normally takes place in association with the peroxisomal membrane, thus paralleling the replication events in infected plant cells. however, previous results with a peroxisome-deficient mutant strain of yeast had suggested that the presence of peroxisomes is not a strict requirement for cymrsv di rna replication. thus, a novel approach was used to study the putative ... | 2007 | 17412997 |
| [production of monoclonal antibodies to cymbidium mosaic virus and application in orchids virus detection]. | three hybridoma cell lines, 2c6, 5b7 and 12g9, secreting monoclonal antibodies (mcabs) against cymbidium mosaic virus (cymmv) were produced by fusing mouse myeloma cells (sp2/0) with spleen cells from balb/ c immunized by the cymmv particles. the three mcabs could specifically react with cymmv. the titres of ascitic fluids of two mcabs are up to 10(-6) in i-elisa. isotypes and subclasses of the the three mcabs belong to igg1. isotypes of light strains of the three mcabs all belong to kappa. they ... | 2007 | 18062276 |
| characterization of hawaiian isolates of cymbidium mosaic virus (cymmv) co-infecting dendrobium orchid. | we report here the isolation and characterization of three distinct isolates of cymbidium mosaic virus (cymmv) co-infecting dendrobium orchid in hawaii. isolates 1 and 2 were phylogenetically distinct from previously reported cymmv isolates. however, isolate 3 was highly similar to previously reported cymmv sequences and could be localised to cymmv subgroup a. isolate 2 localised to cymmv subgroup b. thus, we report here the first full-length cymmv subgroup b isolate. isolate 1 represents a reco ... | 2008 | 18458814 |
| the rx gene confers resistance to a range of potexviruses in transgenic nicotiana plants. | rx-mediated resistance was analyzed in rx-expressing transgenic nicotiana plants. the infection outcome of nine potato virus x isolates mutated at amino acid positions 121 and 127 of the coat protein (cp) confirmed the key role of these amino acids but provided a more complex picture than previously reported. in particular, in rx-expressing nicotiana spp., eliciting activity modulated by amino acid 121 was conditioned by the nature of amino acid 127. these results suggest that the specificity of ... | 2008 | 18700820 |
| phytophthora bisheria sp. nov., a new species identified in isolates from the rosaceous raspberry, rose and strawberry in three continents. | a homothallic semipapillate slow growing phytophthora species associated with root rot of strawberries from greenhouse-grown plants in north carolina, usa, root rot of roses in the netherlands, and root rot of raspberry in knoxfield, australia, was identified. the main character of this organism is the production of paragynous antheridia with broad attachment to the oogonial wall. the morphology of the pathogen does not match that of any of the more than 85 described phytophthora species. phylog ... | 2008 | 18488356 |
| [study on the relationships of cymbidium by rmapd technique]. | cymbidium is one of the orchidaceae genera. there are 70 cymbidium species in the world, mainly distributed over the tropical and the subtropical area of asia and northern australia. in china, approximately 49 species and some variations are distributed over the provinces to the south of qinling. the orchid market of china is quite wide, not only because of their view and admiration value, but also their officinal value as well. some species of cymbidium are morphologically similar, so the ident ... | 2008 | 18567505 |
| cymbidium ringspot virus harnesses rna silencing to control the accumulation of virus parasite satellite rna. | cymbidium ringspot virus (cymrsv) satellite rna (satrna) is a parasitic subviral rna replicon that replicates and accumulates at the cost of its helper virus. this 621-nucleotide (nt) satrna species has no sequence similarity to the helper virus, except for a 51-nt-long region termed the helper-satellite homology (hsh) region, which is essential for satrna replication. we show that the accumulation of satrna strongly depends on temperature and on the presence of the helper virus p19 silencing su ... | 2008 | 18815304 |
| [the genetic diversity of cymbidium by issr]. | issr was applied to detect the relationship between 16 cymbidium species, and 836 bands were amplified with 15 primers, including 227 polymorphic bands. the polymorphic percentage is 27.2%. upgma results showed that the genetic distance were closest between c.goeringii (rchb.f.) rchb.f. and c. goeringii var. longibracteatum, and c.lancifolium hook. was far away from the other 15 species. this result is quite similar to the traditional classification, indicating that the technique could supplemen ... | 2008 | 18487154 |
| natural phenanthrenes and their biological activity. | the aim of this review is to survey the various naturally occurring phenanthrene compounds that have been isolated from different plants. only one review has previously been published on this topic. gorham (1989) reviewed the structures, biosynthesis, separations and spectroscopy of stilbenes and phenanthrenes. the present study furnishes an overview of the hydroxy or/and methoxy-substituted 9,10-dihydro/phenanthrenes, methylated, prenylated and other monomeric derivatives, dimeric and trimeric ... | 2008 | 18243254 |
| the occurrence of crassulacean acid metabolism in cymbidium (orchidaceae) and its ecological and evolutionary implications. | crassulacean acid metabolism (cam) is one of the photosynthetic pathways regarded as adaptations to water stress in land plants. little is known about correlations among the level of cam activity, environment of habitat, life form, and phylogenetic relationship of a plant group from an evolutionary perspective. we examined these relationships in 18 species of cymbidium (orchidaceae) because the genus shows distinctive diversification of habitats and life forms. the photosynthetic type was classe ... | 2008 | 18246297 |
| cymbidium mosaic potexvirus isolate-dependent host movement systems reveal two movement control determinants and the coat protein is the dominant. | little is known about how plant viruses of a single species exhibit different movement behavior in different host species. two cymbidium mosaic potexvirus (cymmv) isolates, m1 and m2, were studied. both can infect phalaenopsis orchids, but only m1 can systemically infect nicotiana benthamiana plants. protoplast inoculation and whole-mount in situ hybridization revealed that both isolates can replicate in n. benthamiana; however, m2 was restricted to the initially infected cells. genome shuffling ... | 2009 | 19345971 |
| molecular diversity and relationships among cymbidium goeringii cultivars based on inter-simple sequence repeat (issr) markers. | spring orchid (cymbidium goeringii) is a popular flowering plant species. there have been few molecular studies of the genetic diversity and conservation genetics on this species. an assessment of the level of genetic diversity in cultivated spring orchid would facilitate development of the future germplasm conservation for cultivar improvement. in the present study, dna markers of intersimple sequence repeats (issr) were identified and the issr fingerprinting technique was used to evaluate gene ... | 2009 | 19085060 |
| properties of a novel satellite rna associated with tomato bushy stunt virus infections. | the biological and molecular properties of a novel satellite rna (satrna l) associated with tomato bushy stunt virus (tbsv) are described. satrna l consisted of a linear single-stranded rna of 615 nt, lacked significant open reading frames (orfs) and had no sequence identity with the helper genome other than in the 5'-proximal 7 nt and in a central region that is also conserved in all tombusvirus genomic, defective interfering and satellite rnas. secondary-structure analysis showed the presence ... | 2010 | 20484559 |
| in vitro growth and single-leaf photosynthetic response of cymbidium plantlets to super-elevated co2 under cold cathode fluorescent lamps. | to examine the effectiveness of super-elevated (10,000 micromol mol(-1)) co(2) enrichment under cold cathode fluorescent lamps (ccfl) for the clonal propagation of cymbidium, plantlets were cultured on modified vacin and went (vw) medium under 0, 3,000 and 10,000 micromol mol(-1) co(2) enrichment and two levels of photosynthetic photon flux density (ppfd, 45 and 75 micromol m(-2) s(-1)). under high ppfd, 10,000 micromol mol(-1) co(2) increased root dry weight and promoted shoot growth. in additi ... | 2010 | 20094885 |
| isolation and identification of rhizoctonia-like fungi from roots of three orchid genera, paphiopedilum, dendrobium, and cymbidium, collected in chiang rai and chiang mai provinces of thailand. | three orchid genera, paphiopedilum, cymbidium, and dendrobium, are among the most heavily traded ornamental plants in thailand. in this study, 27 isolates of rhizoctonia-like fungi were isolated from root sections of mature orchids in the three orchid genera, collected from diverse horticultural settings in chiang mai and chiang rai provinces of thailand. fungal identification was done by the morphological characterization, the comparison of the internal transcribed spacer and 5.8s ribosomal dna ... | 2010 | 20107843 |
| mycoheterotrophy evolved from mixotrophic ancestors: evidence in cymbidium (orchidaceae). | nutritional changes associated with the evolution of achlorophyllous, mycoheterotrophic plants have not previously been inferred with robust phylogenetic hypotheses. variations in heterotrophy in accordance with the evolution of leaflessness were examined using a chlorophyllous-achlorophyllous species pair in cymbidium (orchidaceae), within a well studied phylogenetic background. | 2010 | 20685727 |
| apis cerana japonica discriminates between floral color phases of the oriental orchid, cymbidium floribundum. | foragers of the japanese honeybee (apis cerana japonica) were attracted by flowers of an oriental orchid (cymbidium floribundum) and were observed to carry the pollinia on their scutella. after the removal of pollinia from the flowers, their labial color changed from white to reddish brown. both artificial removal of pollinia and ethrel treatment of the flowers also induced this labial color change. labia in color-changed flowers showed a decreased reflectance of wavelengths less than 670 nm com ... | 2010 | 21110714 |
| isolation and characterization of 15 microsatellite markers from the spring orchid (cymbidium goeringii) (orchidaceae). | • premise of the study: microsatellite primers were developed for the important and popular ornamental spring orchid, cymbidium goeringii, to investigate its genetic diversity and population genetic structure. • methods and results: using the fast isolation by aflp of sequences containing (fiasco) repeats protocol, 15 primer sets were identified in two wild populations. the number of alleles per locus ranged from 2 to 5, with a mean of 3.3. the observed and expected heterozygosities varied from ... | 2011 | 21613151 |
| comparative analysis of the capacity of tombusvirus p22 and p19 proteins to function as avirulence determinants in nicotiana species. | we have used an agroinfiltration assay for a comparative study of the roles of tombusvirus p22 and p19 proteins in elicitation of hypersensitive response (hr)-like necrosis and the role of p19 in silencing suppression in nicotiana species. the advantage of agroinfiltration rather than expression in plant virus vectors is that putative viral avirulence proteins can be evaluated in isolation, eliminating the possibility of synergistic effects with other viral proteins. we found that tombusvirus p2 ... | 2011 | 20977306 |
| [diversity of siderophore-producing endophytic bacteria of cymbidium goeringii roots]. | in order to obtain the effective plant growth-promoting bacteria, we studied the diversity of siderophore-producing endophytic bacteria in the roots of cymbidium goeringii. | 2011 | 21574379 |
| the complete sequence of cymbidium mosaic virus from vanilla fragrans in hainan, china. | the complete nucleotide sequence of cymbidium mosaic virus (cymmv) isolated from vanilla in hainan province, china was determined for the first time. it comprised 6,224 nucleotides; sequence analysis suggested that the isolate we obtained was a member of the genus potexvirus, and its sequence shared 86.67-96.61% identities with previously reported sequences. phylogenetic analysis suggested that cymmv from vanilla fragrans was clustered into subgroup a and the isolates in this subgroup displayed ... | 2011 | 21442302 |
| Microsatellite markers for the Chinese endangered and endemic orchid Cymbidium tortisepalum (Orchidaceae). | • Premise of the study: Microsatellite primers were developed in the Chinese endangered and endemic orchid, Cymbidium tortisepalum, to investigate its genetic diversity and population genetic structure, and to identify its varieties.• Methods and Results: Using the Fast Isolation by AFLP of Sequences Containing repeats (FIASCO) protocol, 15 primer sets were identified in two wild populations. The number of alleles per locus ranged from two to six, with a mean of 3.5. The observed and expected he ... | 2011 | 22203651 |
| one-step reverse transcription loop-mediated isothermal amplification assay for rapid detection of cymbidium mosaic virus. | cymbidium mosaic virus (cymmv) is the most prevalent orchid virus. a single-tube one-step betaine-free reverse transcription (rt) loop-mediated isothermal amplification (lamp) assay was developed for the rapid and easy detection of orchid-infecting cymmv. five sets of primers were designed based on the conserved regions among various virus isolates. the specificity and the sensitivity of the assay were then evaluated using the rt-lamp reaction. within 1h under isothermal conditions at 60°c the t ... | 2011 | 21237208 |
| duplicated c-class mads-box genes reveal distinct roles in gynostemium development in cymbidium ensifolium (orchidaceae). | the orchid floral organs represent novel and effective structures for attracting pollination vectors. in addition, to avoid inbreeding, the androecium and gynoecium are united in a single structure termed the gynostemium. identification of c-class mads-box genes regulating reproductive organ development could help determine the level of homology with the current abc model of floral organ identity in orchids. in this study, we isolated and characterized two c-class agamous-like genes, denoted cem ... | 2011 | 21278368 |
| single primer amplification reaction (spar) reveals inter- and intra-specific natural genetic variation in five species of cymbidium (orchidaceae). | a total of 53 primers belonging to three spar methods, viz. rapd, issr and damd, collectively produced 456 polymorphic amplicons with 96.6% polymorphism at inter-specific level in five species of cymbidium, viz. c. aloifolium, c. mastersii, c. elegans, c. eburneum and c. tigrinum, whereas at intra-specific level, the observed polymorphism ranged from 51.2% to 77.1% among them. three spars collectively revealed 25 unique species-specific amplicons; most of them were amplified with rapd and damd p ... | 2011 | 21641975 |
| genetic variability in the coat protein genes of cymbidium mosaic virus isolates from orchids. | the variability in the nucleotide (nt) and amino acid (aa) sequences of the coat protein (cp) of cymbidium mosaic virus (cymmv), which naturally infects orchids worldwide, was investigated. the cp genes of 55 cymmv isolates originating from different locations in korea were amplified using rt-pcr and sequenced. the encoded cp consists of 223 aa. the cp sequences of the korean isolates were compared with those of previously published cymmv isolates originating from different countries at both nt ... | 2011 | 22015427 |
| Significant demographic and fine-scale genetic structure in expanding and senescing populations of the terrestrial orchid Cymbidium goeringii (Orchidaceae). | • Premise of the study: Fine-scale genetic structure (FSGS) in plants is influenced by variation in spatial and temporal demographic processes. To determine how demographic structure and FSGS change with stages of population succession, we studied replicate expanding and senescing populations of the Asian terrestrial orchid Cymbidium goeringii. • Methods: We used spatial autocorrelation methods (O-ring and kinship statistics) to quantify spatial demographic structure and FSGS in two expanding an ... | 2011 | 22106436 |
| antinociceptive and anti-inflammatory activity of the ethanolic extract of cymbidium aloifolium (l.). | the ethanol leaf extract of cymbidium aloifolium (l.) was evaluated for its analgesic and antiinflammatory activities. the extract, at the dose of 200 and 400 mg kg(-1) body weight, exerted the analgesic activity by observing the number of abdominal contractions and anti-inflammatory activity against carrageenin induced paw edema in mice by measuring the paw volume. the ethanolic extract of cymbidium aloifolium (l.) showed statistically significant (p < 0.05) reduction of percentage of writhing ... | 2011 | 22518936 |
| in vitro expression and production of antibody against cymbidium mosaic virus coat protein. | polyclonal rabbit antisera were produced using coat protein of cymbidium mosaic virus (cymmv) indian isolate expressed in e. coli as gst fusion. the expressed protein was purified by gst-fusion protein purification kit for use as an immunogen in rabbits. antisera prepared in this manner reacted in double antibody sandwich enzyme-linked immunosorbent assay (das elisa) with extract from cymmv-infected tissue. the results indicate that polyclonal antisera prepared from expressed cymmv coat proteins ... | 2012 | 23730003 |
| assessment of phylogenetic inter-relationships in the genus cymbidium (orchidaceae) based on internal transcribed spacer region of rdna. | sequence data obtained from nrits region were used to assess phylogenetic inter-relationships and infrageneric classification of ten cymbidium species collected from north-east india. the final aligned data matrix of combined its 1, 5.8s and its 2 yielded 684 characters. the its 1 and its 2 regions showed variable sequence lengths and g+c content (%). the 5.8s region was found to be more conserved (98.71%) followed by its 1 (86.12%) and its 2 (69.40%). its 2 recorded highest percentage of parsim ... | 2012 | 22245612 |
| first report of sclerotium rot on cymbidium orchids caused by sclerotium rolfsii in korea. | sclerotium rot was found on cymbidium orchids at seosan-si, chungcheongnam-do, korea, in july, 2010. symptoms occurred on low leaves, which turned yellowish, after which the entire plant wilted. severely infected plants were blighted and eventually died. white mycelial mats and sclerotia appeared on pseudobulbs. based on the mycological characteristics and pathogenicity, the causal fungus was identified as sclerotium rolfsii. this is the first report of new sclerotium rot on cymbidium spp. cause ... | 2012 | 23323053 |
| optimizing virus-induced gene silencing efficiency with cymbidium mosaic virus in phalaenopsis flower. | virus-induced gene silencing (vigs) is a good way to study floral gene functions of orchids, especially those with a long life cycle. to explore the applicability and improve viral silencing efficiency for application of cymbidium mosaic virus (cymmv)-induced gene silencing, we examined several variables, including the optimal length of the dna fragment, the effect of developmental maturation status of inflorescence, and suitable inoculation sites. a cymmv-based vigs system can be used with orch ... | 2012 | 23352400 |