Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| pyrimidine nucleoside catabolism in sphingomonas paucimobilis: role of cytidine deaminase and uridine phosphorylase. | pyrimidine nucleoside catabolism in the human pathogen sphingomonas paucimobilis was studied. it was observed that s. paucimobilis was only capable of utilizing cytidine or deoxycytidine as a sole nitrogen source when glucose served as the carbon source. thinlayer chromatographic analyses of cytidine and uridine catabolic products revealed that the enzymes cytidine deaminase and uridine phosphorylase were active in the extracts prepared from s. paucimobilis cells. the levels of cytidine deaminas ... | 1995 | 7600008 |
| microbiological degradation of the herbicide dicamba. | pseudomonas paucimobilis was isolated from a consortium which was capable of degrading dicamba (3,6-dichloro-2-methoxybenzoic acid) as the sole source of carbon. the degradation of dicamba by p. paucimobilis and the consortium was examined over a range of substrate concentration, temperature, and ph. in the concentration range of 100-2000 mg dicamba l-1 (0.5-9.0 mm), the degradation was accompanied by a stoichiometric release of 2 mol of cl- per mol of dicamba degraded. the cultures had an optim ... | 1995 | 7612214 |
| screening and characterization of microorganisms with glutaryl-7adca acylase activity. | a screening of microorganisms producing glutaryl-7adca acylase, an enzyme able to hydrolyse glutaric acid selectively from glutaryl-3-deacetoxy-7-aminocephalosporanic acid (glutaryl-7adca), has been carried out in soil samples. five microorganisms expressing acylase activity were isolated and classified as bacillus cereus, achromobacter xylosooxidans, bacillus sp., pseudomonas sp. and pseudomonas paucimobilis. the screening was carried out by preparing enrichment cultures containing glutaryl-7-a ... | 1995 | 7632401 |
| bacterial contamination of cellular blood components. a retrospective review at a large cancer center. | concern over increased bacterial contamination prompted us to conduct a retrospective review of all bacterial cultures performed on cellular blood components at our institution between december 1989 and june 1993. sterility checks were accomplished by using the bactec blood culture system. the breakdown of the units cultured versus units produced was as follows: packed red blood cells (prbcs): 626 (0.6 percent)/102,593; random donor platelets (rdps): 523 (0.6 percent)/95,005; and single donor pl ... | 1995 | 7668812 |
| cloning of a lignostilbene-alpha,beta-dioxygenase isozyme gene from pseudomonas paucimobilis tmy1009. | a genomic dna library of pseudomonas paucimobilis tmy1009 was constructed using a cosmid vector pwe15. screening of the library for lignostilbene-alpha,beta-dioxygenase (lsd) isozyme genes was done with a common probe for the alpha, beta, and gamma subunits, which composed lsd isozymes. the positive clones obtained by colony hybridization were further confirmed by southern hybridization. a 4.2-kb bamhi-hindiii fragment hybridized with the probe was subcloned into puc118 to yield the plasmid pkhe ... | 1995 | 8534977 |
| stimulation of phagocytosis and phagosome-lysosome fusion by glycosphingolipids from sphingomonas paucimobilis. | sphingomonas paucimobilis, a gram-negative opportunistic pathogen, is actively phagocytosed by human peripheral polymorphonuclear leukocytes (pmn) in vitro. however, when live or killed cells were delipidated, the phagocytic rate was clearly decreased. therefore, we have investigated the physiological role of membrane lipids in phagocytic processes. s. paucimobilis type strain 2395 produces four classes of acidic glycosphingolipids (gl-1, gl-2, gl-3, and gl-4) with the common components of glucu ... | 1995 | 8543558 |
| characterization of pseudomonas paucimobilis fp2001 which forms flagella depending upon the presence of rhamnose in liquid medium. | a bacterial strain fp2001 isolated from the exudate of land reclaimed for municipal waste was identified as pseudomonas paucimobilis. cells of strain fp2001 were mobile by means of polar monotrichous flagellum, only when rhamnose was added as a carbon source in the liquid medium. the replacement of rhamnose by arabinose, galactose, glucose or xylose did not lead to the formation of flagella. | 1995 | 8569527 |
| studies on a viscous, gel-forming exopolysaccharide from sphingomonas paucimobilis gs1. | a new strain, sphingomonas paucimobilis gs1, accumulated 6.5 g of a highly viscous exopolysaccharide per liter, using sucrose as a substrate. the anionic heteropolysaccharide contained the following, in grams per gram: glucose, 0.7; galacturonic acid, 0.11; glucuronic acid, 0.07; and acetate, 0.12. the viscosity of the exopolysaccharide (4.0 g/liter; 4,200 cp) was 5.5 times that of xanthan gum and was stable over a wide ph and temperature range as well as in the presence of nacl. deacetylated po ... | 1995 | 16534966 |
| kinetics of chromate reduction during naphthalene degradation in a mixed culture. | a mixed culture of bacillus sp. k1 and sphingomonas paucimobilis epa 505 was exposed to chromate and naphthalene. batch experiments showed that chromate was reduced and naphthalene was degraded by the mixed culture. chromate reduction occurred initially at a high rate followed by a decrease in rate until chromate reduction ceased. chromate reduction decreased in the mixed culture when a lower ratio of s. paucimobilis epa 505 to bacillus sp. k1 was utilized. a kinetic model incoporating a term fo ... | 1996 | 18629905 |
| halopicolinic acids, novel products arising through the degradation of chloro- and bromo-biphenyl by sphingomonas paucimobilis bpsi-3. | sphingomonas paucimobilis bpsi-3 was previously isolated from a mixed microbial consortium growing on biphenyl as the sole source of carbon and energy. transformation of 4-chlorobiphenyl (4cbp) was demonstrated by this strain, although little or no growth was observed. in minimal salts medium supplemented with 4cbp or bromobiphenyl and dextrose, yellow coloured product(s) were rapidly formed. gas chromatography-mass spectrometry (gc-ms) revealed single-ring n-heterocyclic compounds that were ide ... | 1996 | 8595598 |
| novel polypeptides induced by the insecticide lindane (gamma-hexachlorocyclohexane) are required for its biodegradation by a sphingomonas paucimobilis strain. | when exposed to the potent insecticide gamma-hexachlorocyclohexane or lindane, a sphingomonas paucimobilis strain rapidly synthesized 7 novel polypeptides and concomitantly gained the ability to degrade lindane. synthesis of these proteins was switched-off subsequent to the disappearance of lindane from the medium. treatments which induced the synthesis of identical proteins also conferred on cells the ability to degrade lindane. in contrast, cells blocked in protein synthesis could not be induc ... | 1996 | 8630034 |
| direct involvement of hydrogen peroxide in bacterial alpha-hydroxylation of fatty acid. | we have reported that fatty-acid alpha-hydroxylase partially purified from sphingomonas paucimobilis required nadh and molecular oxygen. in this study, we found that the reaction was greatly inhibited by catalase. glutathione and glutathione peroxidase also inhibited alpha-hydroxylation, but superoxide dismutase and mannitol did not. replacement of nadh and molecular oxygen by hydrogen peroxide increased the alpha-hydroxylation activity. in the presence of hydrogen peroxide, molecular oxygen was ... | 1996 | 8647293 |
| bacterial degradation of low concentrations of phenanthrene and inhibition by naphthalene | phenanthrene-degrading bacteria were isolated from enrichment cultures of soils contaminated with creosote and jet fuel. the isolates from the creosote enrichments were classified by fatty acid methyl ester profiles as acidovorax delafieldii and sphingomonas paucimobilis; the bacterium from the jet fuel-contaminated soil was not identified and was designated strain jfd11. all three isolates used phenanthrene as a sole carbon and energy source, and two of the isolates used fluoranthene as a sole ... | 1996 | 8661535 |
| tracheal colonization with sphingomonas paucimobilis in mechanically ventilated neonates due to contaminated ventilator temperature probes. | sphingomonas paucimobilis was isolated from tracheal secretions of a total of 85 mechanically ventilated babies in a neonatal intensive-care unit (nicu) during a two-year-period. none of the neonates developed pneumonia or sepsis. after each increase in the fluctuating number of s. paucimobilis isolates, extra attention was paid to hand hygiene and to the maintenance of the ventilation equipment. this resulted in a reduction of the frequency of isolation each time. cultures of all liquids in use ... | 1996 | 8690883 |
| catechol dioxygenases from escherichia coli (mhpb) and alcaligenes eutrophus (mpci): sequence analysis and biochemical properties of a third family of extradiol dioxygenases. | the nucleotide sequence of the escherichia coli mhpb gene, encoding 2,3-dihydroxyphenylpropionate 1,2-dioxygenase, was determined by sequencing of a 3.1-kb fragment of dna from kohara phage 139. the inferred amino acid sequence showed 58% sequence identity with the sequence of an extradiol dioxygenase, mpci, from alcaligenes eutrophus and 10 to 20% sequence identity with protocatechuate 4,5-dioxygenase from pseudomonas paucimobilis, with 3,4-dihydroxyphenylacetate 2,3-dioxygenase from e. coli, a ... | 1996 | 8752345 |
| planar asymmetric lipid bilayers of glycosphingolipid or lipopolysaccharide on one side and phospholipids on the other: membrane potential, porin function, and complement activation. | we have determined some physicochemical properties of the monosaccharide-type fraction (gsl-1) of glycosphingolipids, the major glycolipid components of the outer leaflet of the gram-negative species sphingomonas paucimobilis. these properties included the state of order of the hydrocarbon moiety, the effective molecular area, surface charge density, and intrinsic transmembrane potential profile of reconstituted planar asymmetric gsl-1/phospholipid bilayer membranes. we have, furthermore, invest ... | 1996 | 8770208 |
| isolation and characterization of a novel gamma-hexachlorocyclohexane-degrading bacterium. | the natural biotic capacity of soils to degrade gamma-hexachlorocyclohexane (gamma-hch, lindane) was estimated using an enrichment technique based on the ability of soil bacteria to develop on synthetic media and degrade the xenobiotic compound, used as the sole source of carbon and energy. bacterial inocula from relatively highly contaminated soils (from wood treatment factories) were found to promote efficiently the degradation of gamma-hch, which subsequently permitted isolation of a competen ... | 1996 | 8830705 |
| description of chlorophenol-degrading pseudomonas sp. strains kf1t, kf3, and nkf1 as a new species of the genus sphingomonas, sphingomonas subarctica sp. nov. | gram-negative polychlorophenol-degrading bacterial strains kf1t (t = type strain), kf3, and nkf1, which were described previously as pseudomonas saccharophila strains, were studied by chemotaxonomic, genetic, and physiological methods and by electron microscopy and compared with selected xenobiotic compound-degrading bacteria. these strains contained sphingolipids with d-18:0, d-20:1, and d-21:1 as the main dihydrosphingosines, ubiquinone 10 as the main respiratory quinone, and spermidine as the ... | 1996 | 8863434 |
| isolation and characterization of soil microorganisms capable of utilizing the herbicide diclofop-methyl as a sole source of carbon and energy. | six nonfermentative gram-negative bacilli were isolated from manitoban soils after enrichment with diclofopmethyl. microscopic examination and physiological and biochemical tests have identified the organisms as sphingomonas paucimobilis, acinetobacter baumannii, chryseomonas luteola, pseudomonas aureofaciens, pseudomonas cepacia, and pseudomonas fluorescens. growth curve studies showed that each of the isolates was able to grow in minimal medium with diclofop-methyl as the sole source of carbon ... | 1996 | 8868228 |
| degradation of diclofop-methyl by pure cultures of bacteria isolated from manitoban soils. | pure cultures of chryseomonas luteola and sphingomonas paucimobilis isolated from manitoban soils were able to utilize diclofop-methyl (methyl-2-[4-(2,4-dichlorophenoxy)phenoxy] propanoate) as the sole source of carbon and energy. an actively growing culture of c. luteola completely degraded 1.5 micrograms diclofop-methyl.ml-1 to diclofop acid and 4-(2,4-dichlorophenoxy)phenol within 71 h, as determined by gas chromatographic analysis. the accumulation of these metabolites in the growth medium r ... | 1996 | 8868229 |
| bacteremia due to glucose non-fermenting gram-negative bacilli in patients with hematological neoplasias and solid tumors. | twenty-six patients with hematological or solid tumors who developed bacteremia caused by stenotrophomonas maltophilia (n = 10), pseudomonas putida (n = 6), sphingomonas paucimobilis complex (n = 4) or alcaligenes xylosoxidans (n = 6) in the period between 1993 and 1995 were studied. seventeen patients were neutropenic during the infection, and 13 were undergoing bone marrow or peripheral blood stem cell transplantation. twenty-three patients had catheter-related infections; only 3 of the 26 pat ... | 1996 | 8874083 |
| structural studies of the exocellular polysaccharide from sphingomonas paucimobilis strain i-886. | the exocellular polysaccharide from sphingomonas paucimobilis strain i-886 has been studied using methylation analysis, smith degradation, partial acid hydrolysis, nmr spectroscopy, and mass spectrometry as the principal methods. it is concluded that the repeating unit has the following structure: [formula: see text] the absolute configuration of the uronic acid was deduced from 1h nmr chemical shifts and is most likely d. some preparations of the polysaccharide also contain phosphate and o-acyl ... | 1996 | 9011378 |
| computer-aided comparison of protein electrophoretic patterns for grouping and identification of heterotrophic bacteria from mineral water. | the microflora of a natural mineral water was studied immediately after bottling (t0) and after 7 d storage (t7) during 6 months, and isolates were clustered by sds-page of whole-cell protein profiles. isolates from each cluster were further characterized by api 20ne, fatty acid composition and quinone profiles. the numerical analysis of the electrophoregrams of all bacteria isolated from the mineral water formed 15 clusters and five unclustered strains. except for five minor clusters, all clust ... | 1996 | 9072519 |
| phylogenetic and physiological comparisons of pah-degrading bacteria from geographically diverse soils. | the diversity of bacteria isolated from creosote- contaminated soils in the united states, norway, and germany was determined by comparing their ability to degrade polycyclic aromatic hydrocarbons (pahs), their phospholipid ester-linked fatty acid (gc-fame) profiles, sole carbon source utilization patterns (biolog assays), and 16s rrna sequences. bacteria were initially obtained by enrichment with phenanthrene and fluoranthene. many were capable of degrading a broad range of the pahs found in cr ... | 1997 | 9195008 |
| glutathione s-transferase-encoding gene as a potential probe for environmental bacterial isolates capable of degrading polycyclic aromatic hydrocarbons. | homologs of the glutathione s-transferase (gst)-encoding gene were identified in a collection of aromatic hydrocarbon-degrading sphingomonas spp. isolated from new zealand, antarctica, and the united states by using pcr primers designed from the gst-encoding gene of sphingomonas paucimobilis epa505. sequence analysis of pcr fragments generated from these isolates and of the gst gene amplified from dna extracted from polycyclic aromatic hydrocarbon (pah)-contaminated soil revealed a high degree o ... | 1997 | 9251217 |
| purification and characterization of a haloalkane dehalogenase of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, sphingomonas paucimobilis ut26. | the linb gene product (linb), 1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase, which is involved in the degradation of gamma-hexachlorocyclohexane in sphingomonas paucimobilis ut26 (y. nagata, t. nariya, r. ohtomo, m. fukuda, k. yano, and m. takagi, j. bacteriol. 175:6403-6410, 1993), was overproduced in e. coli and purified to homogeneity. the molecular mass of linb was deduced to be 30 kda by gel filtration chromatography and 32 kda by electrophoresis on sodium dodecyl sulfate-polyacryl ... | 1997 | 9293022 |
| molecular cloning and expression of fatty acid alpha-hydroxylase from sphingomonas paucimobilis. | fatty acid alpha-hydroxylase (faah) catalyzes the initial reaction in alpha-oxidation of fatty acid to produce 2-hydroxy fatty acid. faah activity has been detected in a wide range of organisms from prokaryotes to eukaryotes. here, we describe cloning of the faah gene from sphingomonas paucimobilis, a sphingolipid- and 2-hydroxymyristic acid-rich bacterium. the isolated gene encoded 415 amino acids. a homology search revealed that amino acid sequences highly conserved in cytochrome p450 (p450) w ... | 1997 | 9295298 |
| purification and some properties of lignostilbene-alpha, beta-dioxygenase isozyme iv from pseudomonas paucimobilis tmy1009. | lignostilbene-alpha, beta-dioxygenase isozyme iv was purified from ultrasonic extracts of pseudomonas paucimobilis tmy1009 through four steps of column chromatography. the fraction obtained gave a single band on sds-page and a single peak on reversed-phase hplc and deae-hplc. the specific activity and km of purified isozyme iv were 110 mu kat/g and 4.2 microm for 4.4'-dihydroxy-3,3'-dimethoxystilbene, 150 mu kat/g and 3.3 microm for 4,2'-dihydroxy-3,3'-dimethoxy-5'-(2"-carboxyvinyl)stilbene. the ... | 1997 | 9339561 |
| emergence of cu(++)-tolerant mutants defective in gellan synthesis in cu(++)-stressed cultures of sphingomonas paucimobilis. | cells defective in gellan synthesis appeared during cultivation of the gellan gum-producing strain sphingomonas paucimobilis r40 with inhibitory concentrations of copper, supplied as cucl2. the percentage of less mucoid colonial variants dramatically increased with the increase in cu++ supplementation, reaching 85% of total viable cells at the maximal concentration for growth. results reported in this work indicate that emergence of colonial variants defective in gellan synthesis results from cu ... | 1997 | 9765805 |
| [bacteremia caused by sphingomonas (pseudomonas) paucimobilis]. | 1998 | 9835164 | |
| phytanic acid alpha-hydroxylation by bacterial cytochrome p450. | fatty acid alpha-hydroxylase, a cytochrome p450 enzyme, from sphingomonas paucimobilis, utilizes various straight-chain fatty acids as substrates. we investigated whether a recombinant fatty acid alpha-hydroxylase is able to metabolize phytanic acid, a methyl-branched fatty acid. when phytanic acid was incubated with the recombinant enzyme in the presence of h2o2, a reaction product was detected by gas chromatography, whereas a reaction product was not detected in the absence of h2o2. when a hea ... | 1998 | 9930407 |
| impact of inoculation protocols, salinity, and ph on the degradation of polycyclic aromatic hydrocarbons (pahs) and survival of pah-degrading bacteria introduced into soil. | degradation of polycyclic aromatic hydrocarbons (pahs) and survival of bacteria in soil was investigated by applying different inoculation protocols. the soil was inoculated with sphingomonas paucimobilis ba 2 and strain bp 9, which are able to degrade anthracene and pyrene, respectively. cfu of soil bacteria and of the introduced bacteria were monitored in native and sterilized soil at different phs. introduction with mineral medium inhibited pah degradation by the autochthonous microflora and ... | 1998 | 9435090 |
| characterization of marine temperate phage-host systems isolated from mamala bay, oahu, hawaii. | to understand the ecological and genetic role of viruses in the marine environment, it is critical to know the infectivity of viruses and the types of interactions that occur between marine viruses and their hosts. we isolated four marine phages from turbid plaques by using four indigenous bacterial hosts obtained from concentrated water samples from mamala bay, oahu, hawaii. two of the rod-shaped bacterial hosts were identified as sphingomonas paucimobilis and flavobacterium sp. all of the phag ... | 1998 | 9464390 |
| cloning and sequencing of the sphingomonas (pseudomonas) paucimobilis gene essential for the o demethylation of vanillate and syringate. | sphingomonas (pseudomonas) paucimobilis syk-6 is able to grow on 5,5'-dehydrodivanillic acid (ddva), syringate, vanillate, and other dimeric model compounds of lignin as a sole carbon source. nitrosoguanidine mutagenesis of s. paucimobilis syk-6 was performed, and two mutants with altered ddva degradation pathways were isolated. the mutant strain nt-1 could not degrade ddva, but could degrade syringate, vanillate, and 2,2',3'-trihydroxy-3-methoxy-5,5'-dicarboxybiphenyl (oh-ddva). strain dc-49 co ... | 1998 | 9501423 |
| microbial contamination of 'sterile water' used in japanese hospitals. | we examined the following samples of water from 10 hospitals for microbial contamination: water obtained using an ultra filtration system (uf water), a reverse osmosis system (ro water), a water distillation system (distilled water) and tap water. uf water and ro water are used for handwashing before surgery, and distilled water for the preparation of drugs. all 10 samples of tap water examined were contaminated with < 10 colony forming units (cfu)/ml. thirteen (68%) of 19 samples of uf water, n ... | 1998 | 9513069 |
| cloning and sequencing of a 2,5-dichlorohydroquinone reductive dehalogenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane by sphingomonas paucimobilis. | sphingomonas (formerly pseudomonas) paucimobilis ut26 utilizes gamma-hexachlorocyclohexane (gamma-hch), a halogenated organic insecticide, as a sole carbon and energy source. in a previous study, we showed that gamma-hch is degraded to 2,5-dichlorohydroquinone (2,5-dchq) (y. nagata, r. ohtomo, k. miyauchi, m. fukuda, k. yano, and m. takagi, j. bacteriol. 176:3117-3125, 1994). in the present study, we cloned and characterized a gene, designated lind, directly involved in the degradation of 2,5-dc ... | 1998 | 9515900 |
| nosocomial infections caused by sphingomonas paucimobilis: clinical features and microbiological characteristics. | from january 1995 to september 1996, 14 isolates of sphingomonas paucimobilis, including 11 from clinical specimens from six patients with nosocomial infection and three from environmental sources, were collected. two of the six patients had intravascular catheter-related bacteremia and one each had bacteremic biliary tract infection, urinary tract infection, ventilator-associated pneumonia, and wound infection. the s. paucimobilis isolates were identified according to biochemical profiles estab ... | 1998 | 9524843 |
| molecular mechanisms of polymyxin b-membrane interactions: direct correlation between surface charge density and self-promoted transport. | we have studied the interaction of the polycationic peptide antibiotic polymyxin b (pmb) with asymmetric planar bilayer membranes via electrical measurements. the bilayers were of different compositions, including those of the lipid matrices of the outer membranes of various species of gram-negative bacteria. one leaflet, representing the bacterial inner leaflet, consisted of a phospholipid mixture (pl; phosphatidylethanolamine, -glycerol, and diphosphatidylglycerol in a molar ratio of 81:17:2). ... | 1998 | 9538506 |
| oxidation of methyl-substituted naphthalenes: pathways in a versatile sphingomonas paucimobilis strain | aromatic compounds with alkyl substituents are abundant in fossil fuels. these compounds become important environmental sources of soluble toxic products, developmental inhibitors, etc. principally through biological activities. to assess the effect of methyl substitution on the completeness of mineralization and accumulation of pathway products, an isolate from a phenanthrene enrichment culture, sphingomonas paucimobilis 2322, was used. washed cell suspensions containing cells grown on 2,6-dime ... | 1998 | 9572967 |
| [bacteremia by sphingomonas paucimobilis]. | 1998 | 9586371 | |
| further characterization of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from sphingomonas paucimobilis. | although fatty acid alpha-hydroxylase (faah) activity has been detected in various species, faah has not been sufficiently characterized. in this report, we describe the properties of faah highly purified from sphingomonas paucimobilis. the faah was purified by about 5,200-fold. blotting analysis with a specific antibody against the faah showed that its apparent molecular mass was approximately 43 kda. faah showed alpha-hydroxylation activity in the presence of h2o2, but little if any activity w ... | 1998 | 9644252 |
| cloning of a sphingomonas paucimobilis syk-6 gene encoding a novel oxygenase that cleaves lignin-related biphenyl and characterization of the enzyme. | sphingomonas paucimobilis syk-6 transforms 2,2'-dihydroxy-3,3'-dimethoxy-5,5'-dicarboxybiphenyl (ddva), a lignin-related biphenyl compound, to 5-carboxyvanillic acid via 2,2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (oh-ddva) as an intermediate (15). the ring fission of oh-ddva is an essential step in the ddva degradative pathway. a 15-kb ecori fragment isolated from the cosmid library complemented the growth deficiency of a mutant on oh-ddva. subcloning and deletion analysis showed that a ... | 1998 | 9647824 |
| alpha-l-rhamnosidase of sphingomonas sp. r1 producing an unusual exopolysaccharide of sphingan. | a soil bacterium with alpha-l-rhamnosidase was isolated from a cumulative mixed culture containing a polysaccharide of gellan as a carbon source and identified to be sphingomonas paucimobilis, known as a potent producer of gellan. the isolate (designated sphingomonas sp. r1) produced an unusual exopolysaccharide of sphingan (denoted hwr1) distinct from gellan. the rhamnose in gellan was replaced with mannose in hwr1. the bacterium had a peculiar cell surface covered with many complicated plaits. ... | 1998 | 9692187 |
| effect of calcium on the surfactant tolerance of a fluoranthene degrading bacterium. | surfactants are known to increase the apparent aqueous solubility of polycyclic aromatic hydrocarbons (pahs) and may thus be used to enhance the bioavailability and thereby to stimulate the biodegradation of these hydrophobic compounds. however, surfactants may in some cases reduce or inhibit biodegradation because of toxicity to the bacteria. in this study, toxicity of surfactants on sphingomonas paucimobilis strain epa505 and the effect on fluoranthene mineralization were investigated using tr ... | 1998 | 10192897 |
| crystallization and preliminary x-ray diffraction analysis of haloalkane dehalogenase linb from sphingomonas paucimobilis ut26. | haloalkane hydrolytic dehalogenase linb from sphingomonas paucimobilis ut26, an enzyme which releases chloride or bromide anion from n-halogenated alkanes and has a broad range of substrate specificity, was crystallized using the hanging-drop vapour-diffusion method at 278 k. the best crystals were obtained by microseeding with a precipitant containing 18-20%(w/v) peg 6000, 0.2 m calcium acetate and 0.1 m tris-hcl ph 8.9. the crystals diffract to at least 1.60 a using synchrotron x-ray under cry ... | 1999 | 10329794 |
| structures and properties of gellan polymers produced by sphingomonas paucimobilis atcc 31461 from lactose compared with those produced from glucose and from cheese whey | the dairy industry produces large quantities of whey as a by-product of cheese production and is increasingly looking for new ways to utilize this waste product. gellan gum is reliably produced by sphingomonas paucimobilis in growth media containing lactose, a significant component of cheese whey, as a carbon source. we studied and compared polysaccharide biosynthesis by s. paucimobilis atcc 31461 in media containing glucose, lactose (5 to 30 g/liter), and sweet cheese whey. we found that alteri ... | 1999 | 10347031 |
| enhancement of solubilization and biodegradation of polyaromatic hydrocarbons by the bioemulsifier alasan. | alasan, a high-molecular-weight bioemulsifier complex of an anionic polysaccharide and proteins that is produced by acinetobacter radioresistens ka53 (s. navon-venezia, z. zosim, a. gottlieb, r. legmann, s. carmeli, e. z. ron, and e. rosenberg, appl. environ. microbiol. 61:3240-3244, 1995), enhanced the aqueous solubility and biodegradation rates of polyaromatic hydrocarbons (pahs). in the presence of 500 microg of alasan ml-1, the apparent aqueous solubilities of phenanthrene, fluoranthene, and ... | 1999 | 10347063 |
| characterization of the meta-cleavage compound hydrolase gene involved in degradation of the lignin-related biphenyl structure by sphingomonas paucimobilis syk-6. | sphingomonas paucimobilis syk-6 has the ability to transform a lignin-related biphenyl compound, 2,2'-dihydroxy-3,3'-dimethoxy-5, 5'-dicarboxybiphenyl (ddva), to 5-carboxyvanillic acid (5cva) via 2, 2',3-trihydroxy-3'-methoxy-5,5'-dicarboxybiphenyl (oh-ddva). in the 4.9-kb hindiii fragment containing the oh-ddva meta-cleavage dioxygenase gene (ligz), we found a novel hydrolase gene (ligy) responsible for the conversion of the meta-cleavage compound of oh-ddva to 5cva. incorporation of 18o from h ... | 1999 | 10347082 |
| survival of enterobacter cloacae and pseudomonas paucimobilis in yoghurts manufactured from cow's milk and soymilk during storage at different temperatures. | the survival of two microbial contaminants, enterobacter cloacae and pseudomonas paucimobilis, in yoghurts manufactured from cow's milk and soymilk was investigated during storage for 45 days at 4 and 12 degrees c. sensory panel tests performed before microbiological investigation, showed that the flavor of soy-yoghurts made with cocoa powder or malt added did not have the beany taste of soy beans. both contaminants were significantly resistant to low ph values during storage for 32 days at 4 de ... | 1999 | 10356792 |
| two different types of dehalogenases, lina and linb, involved in gamma-hexachlorocyclohexane degradation in sphingomonas paucimobilis ut26 are localized in the periplasmic space without molecular processing. | gamma-hexachlorocyclohexane (gamma-hch) is one of several highly chlorinated insecticides that cause serious environmental problems. the cellular proteins of a gamma-hch-degrading bacterium, sphingomonas paucimobilis ut26, were fractionated into periplasmic, cytosolic, and membrane fractions after osmotic shock. most of two different types of dehalogenase, lina (gamma-hexachlorocyclohexane dehydrochlorinase) and linb (1,3,4,6-tetrachloro-1,4-cyclohexadiene halidohydrolase), that are involved in ... | 1999 | 10464214 |
| crystal structure of an aromatic ring opening dioxygenase ligab, a protocatechuate 4,5-dioxygenase, under aerobic conditions. | sphingomonas paucimobilis syk-6 utilizes an extradiol-type catecholic dioxygenase, the ligab enzyme (a protocatechuate 4,5-dioxygenase), to oxidize protocatechuate (or 3,4-dihydroxybenzoic acid, pca). the enzyme belongs to the family of class iii extradiol-type catecholic dioxygenases catalyzing the ring-opening reaction of protocatechuate and related compounds. the primary structure of ligab suggests that the enzyme has no evolutionary relationship with the family of class ii extradiol-type cat ... | 1999 | 10467151 |
| characterization of the ybdt gene product of bacillus subtilis: novel fatty acid beta-hydroxylating cytochrome p450. | we have characterized the gene encoding fatty acid alpha-hydroxylase, a cytochrome p450 (p450) enzyme, from sphingomonas paucimobilis. a database homology search indicated that the deduced amino acid sequence of this gene product was 44% identical to that of the ybdt gene product that is a 48 kda protein of unknown function from bacillus subtilis. in this study, we cloned the ybdt gene and characterized this gene product using a recombinant enzyme to clarify function of the ybdt gene product. th ... | 1999 | 10529095 |
| cloning and sequencing of a novel meta-cleavage dioxygenase gene whose product is involved in degradation of gamma-hexachlorocyclohexane in sphingomonas paucimobilis. | sphingomonas (formerly pseudomonas) paucimobilis ut26 utilizes gamma-hexachlorocyclohexane (gamma-hch), a halogenated organic insecticide, as a sole source of carbon and energy. in a previous study, we showed that gamma-hch is degraded to chlorohydroquinone (chq) and then to hydroquinone (hq), although the rate of reaction from chq to hq was slow (k. miyauchi, s. k. suh, y. nagata, and m. takagi, j. bacteriol. 180:1354-1359, 1998). in this study, we cloned and characterized a gene, designated li ... | 1999 | 10542173 |
| construction and characterization of histidine-tagged haloalkane dehalogenase (linb) of a new substrate class from a gamma-hexachlorocyclohexane-degrading bacterium, sphingomonas paucimobilis ut26. | the linb gene product (linb), which is involved in the degradation of gamma-hexachlorocyclohexane in sphingomonas paucimobilis ut26, is a member of haloalkane dehalogenases with a broad range of substrate specificity. elucidation of the factors determining its substrate specificity is of interest. aiming to facilitate purification of recombinant linb protein for site-directed mutagenesis analysis, a 6-histidyl tail was added to the c-terminus of linb. the his-tagged linb was specifically bound w ... | 1999 | 10545279 |
| effects of glucose and phenylalanine upon 2,4,6-trichlorophenol degradation by pseudomonas paucimobilis s37 cells in a no-growth state. | pseudomonas paucimobilis s37, a strain able to degrade 2,4,6-trichlorophenol (246-tcp), was isolated from an aquatic environment polluted with this compound. the effect of two natural organic compounds on the degradation of 246-tcp by this strain, in a no-growth state, was studied. bacterial cultures were exposed to 0.1 mm and 0.5 mm of 246-tcp, alone, or in the presence of similar concentrations of glucose, a growth supporting substrate, or phenylalanine, a no-growth supporting compound. the ef ... | 1999 | 10581731 |
| rhodanobacter lindaniclasticus gen. nov., sp. nov., a lindane-degrading bacterium. | lindane-degrading activity under aerobic conditions has been observed in two bacterial strains: ut26, phenotypically identified as sphingomonas paucimobilis, and a new single unidentified isolate named rp5557t. the rrs (16s rdna) sequences for both strains and the phenotypic characteristics for the unidentified isolate rp5557t were determined. rp5557t does not have high identity (less than 90% in all cases) with any sequence in the genbank or rdp databases. a phylogenetic analysis based on rrs s ... | 1999 | 10028243 |
| identification of the catalytic triad in the haloalkane dehalogenase from sphingomonas paucimobilis ut26. | the haloalkane dehalogenase from sphingomonas paucimobilis ut26 (linb) is the enzyme involved in the gamma-hexachlorocyclohexane degradation. this enzyme hydrolyses a broad range of halogenated aliphatic compounds via an alkyl-enzyme intermediate. linb is believed to belong to the family of alpha/beta-hydrolases which employ a catalytic triad, i.e. nucleophile-histidine-acid, during the catalytic reaction. the position of the catalytic triad within the sequence of linb was probed by a site-direc ... | 1999 | 10100638 |
| genetic and biochemical characterization of a 2-pyrone-4, 6-dicarboxylic acid hydrolase involved in the protocatechuate 4, 5-cleavage pathway of sphingomonas paucimobilis syk-6. | sphingomonas paucimobilis syk-6 is able to grow on a wide variety of dimeric lignin compounds with guaiacyl moieties, which are converted into protocatechuate by the actions of lignin degradation enzymes in this strain. protocatechuate is a key metabolite in the syk-6 degradation of lignin compounds with guaiacyl moieties, and it is thought that it degrades to pyruvate and oxaloacetate via the protocatechuate 4,5-cleavage pathway. in a 10.5-kb ecori fragment carrying the protocatechuate 4,5-diox ... | 1999 | 9864312 |
| complement activation by bacterial surface glycolipids: a study with planar bilayer membranes. | planar asymmetric glycolipid/phospholipid bilayer membranes were used as a reconstitution model of the lipid matrix of the outer membrane of gram-negative bacteria to study complement (c) activation by various bacterial surface glycolipids with the aim of defining the c activation pathway. as glycolipids the lipopolysaccharides of salmonella enterica serovar minnesota r mutant strains r595 (re lps) and r4 (rd2 lps), pentaacyl lipid a from the lps of the escherichia coli re mutant f515, and glyco ... | 1999 | 9929374 |
| sphingomonas paucimobilis bpsi-3 mutant an2 produces a red catabolite during biphenyl degradation. | the biphenyl degradation pathway of sphingomonas paucimobilis bpsi-3 was investigated using a degradation-deficient mutant generated by 1-methyl-3-nitro-1-nitrosoguanidine (ntg) mutagenesis. the mutant, designated an2, was confirmed as originating from bpsi-3 through the use of eric (enterobacterial repetitive intergenic consensus) pcr and by detection of the diagnostic pigment, nostoxanthin, in cellular methanol extracts. mutant an2 produced a yellow followed by red extracellular substance when ... | 1999 | 11423948 |
| degradation of the phenoxy acid herbicide diclofop-methyl by sphingomonas paucimobilis isolated from a canadian prairie soil. | sphingomonas paucimobilis, isolated from a soil in manitoba, canada, was able to utilize diclofop-methyl, (r,s)-methyl-2-[4-(2,4-dichlorophenoxy)phenoxy]propionate, as the sole source of carbon and energy. an actively growing aerobic culture completely degraded 1.5 &mgr;g diclofop-methyl ml(-1) to diclofop acid within 54 h, at 25 degrees c. a biphasic growth pattern indicated that this organism was capable of degrading diclofop acid to 4-(2,4-dichlorophenoxy)phenol and 2,4-dichlorophenol and/or ... | 1999 | 11423951 |
| 5-chloropicolinic acid is produced by specific degradation of 4-chlorobenzoic acid by sphingomonas paucimobilis bpsi-3. | we have previously shown that the bacterium sphingomonas paucimobilis bpsi-3, isolated from pcb-contaminated soil, can degrade halogenated biphenyls, naphthalenes, catechols and benzoic acids. however, before such an organism can be used in bioremediation, it is important to characterise the degradation products and determine the degradation pathways to ensure that compounds more toxic or mobile than the original contaminants are not produced. in the degradation of 4-chlorobiphenyl, s. paucimobi ... | 1999 | 11423954 |
| characterization of sphingomonas paucimobilis syk-6 genes involved in degradation of lignin-related compounds. | sphingomonas paucimobilis syk-6 is able to grow on a wide variety of dimeric lignin compounds. these compounds are degraded via vanillate and syringate by a unique enzymatic system, composed of etherases, o demethylases, ring cleavage oxygenases and side chain cleaving enzymes. these unique and specific lignin modification enzymes are thought to be powerful tools for utilization of the most abundant aromatic biomass, lignin. here, we focus on the genes and enzymes involved in beta-aryl ether cle ... | 1999 | 11423957 |
| complete analysis of genes and enzymes for gamma-hexachlorocyclohexane degradation in sphingomonas paucimobilis ut26. | gamma-hexachlorocyclohexane (gamma-hch; also called bhc or lindane) is one of the highly chlorinated pesticides which can cause serious environmental problems. sphingomonas paucimobilis ut26 degrades gamma-hch under aerobic conditions. the unique degradation pathway of gamma-hch in ut26 is revealed. in the upstream pathway, gamma-hch is transformed to 2,5-dichlorohydroquinone (2,5-dchq) by two different dehalogenases (lina and linb) and one dehydrogenase (linc) which are expressed constitutively ... | 1999 | 11423959 |
| evaluation of rheological properties of the exopolysaccharide of sphingomonas paucimobilis gs-1 for application in oil exploration. | analysis of an exopolysaccharide of sphingomonas paucimobilis gs-1 (eps/gs-1) with respect to its rheological properties, cross-linking ability with chrome alum and performance test at 75 +/- 5 degrees c revealed its strong suspending ability, shear thinning property, and thixotrophic nature which are required to impart desirable rheology to drilling mud. the organism fulfilled all the specified requirements and its properties were superior to those of currently-used xc polymer (a xanthan produc ... | 1999 | 11423966 |
| application of a floc-forming genetically engineered microorganism to a sequencing batch reactor for phenolic wastewater treatment. | for enhancing the survival of genetically engineered microorganisms (gems) in activated sludge processes, the use of a floc-forming bacterium as the host for a recombinant plasmid was proposed. the floc-forming and phenol-degrading gem sphingomonas paucimobilis 551 (ps10-45) was cultured to demonstrate this proposal. although the maximum growth rate of the host strain s. paucimobilis 551 was low and the recombinant plasmid ps10-45 was unstable in the host, the resultant gem s. paucimobilis 551 ( ... | 1999 | 16232579 |
| biodegradation of dibenzothiophene and 4,6-dimethyldibenzothiophene by sphingomonas paucimobilis strain tzs-7. | strain tzs-7, tentatively identified as sphingomonas paucimobilis, was isolated from crude oil for its ability to degrade dibenzothiophene (dbt) and 4,6-dimethyldibenzothiophene (4,6-dmdbt). this strain did not utilize dbt or 4,6-dmdbt as the sole source of sulfur. however, the degradative activity was induced by various aromatic compounds, including dbt, fluorene, anthracene, naphthalene and toluene. three products formed from 4,6-dmdbt degradation were detected and two of these were proposed t ... | 1999 | 16232614 |
| preferential occurrence of diazotrophic endophytes, azoarcus spp., in wild rice species and land races of oryza sativa in comparison with modern races. | several diazotrophic species of azoarcus spp. occur as endophytes in the pioneer plant kallar grass. the purpose of this study was to screen asian wild rice and cultivated oryza sativa varieties for natural association with these endophytes. populations of culturable diazotrophs in surface-sterilized roots were characterized by 16s rdna sequence analysis, and azoarcus species were identified by genomic fingerprints. a. indigens and azoarcus sp. group c were detected only rarely, whereas azoarcus ... | 2000 | 11220300 |
| horizontal transfer of an exopolymer complex from one bacterial species to another. | alasan, the exocellular polymeric emulsifier produced by acinetobacter radioresistens ka53 was shown to bind to the surface of sphingomonas paucimobilis epa505 and acinetobacter calcoaceticus rag-1. the presence of alasan on the surface of s. paucimobilis epa505 and a. calcoaceticus rag-1 caused a decrease in their cell-surface hydrophobicities. binding was proportional to the concentration of recipient cells and input alasan. at the highest concentration of a. calcoaceticus rag-1 (4 x 10(9) ml( ... | 2000 | 11234924 |
| identification of four structural genes and two putative promoters necessary for utilization of naphthalene, phenanthrene, fluoranthene by sphingomonas paucimobilis var. epa505. | sphingomonas paucimobilis var. epa505 utilizes fluoranthene (fla), naphthalene (nap), and phenanthrene (phe) as sole carbon sources for energy and growth. a genetic library of epa505 was constructed using mini-tn5 promoter reporter genes encoding for tetracycline resistance (tc(p-)) or luminescence (luxab(p-)). out of 2250 tn5 mutants, ten were deficient in utilization of fla, nap, and/or phe as sole carbon sources. three classes of tn5 mutants were defined: classi (nap(-)phe(-)fla(-)), classii ... | 2000 | 11137301 |
| chemotaxonomic and phylogenetic analyses of sphingomonas strains isolated from ears of plants in the family gramineae and a proposal of sphingomonas roseoflava sp. nov. | chemotaxonomic and phylogenetic characteristics of sphingomonas strains isolated from plants of the family gramineae were investigated. all strains contained the monosaccharide (glucuronic acid) type of glycosphingolipid (gsl-1). most were found also to contain the oligosaccharide-type glycosphingolipids. fatty acid and sphingosine profiles of the isolates were identical, although the ratio of the contents varies among the isolates. they all contained ubiquinone q-10, and the g1c contents were f ... | 2000 | 12483599 |
| purification and characterization of intracellular alpha-l-rhamnosidase from pseudomonas paucimobilis fp2001. | alpha-l-rhamnosidase was extracted and purified from the cells of pseudomonas paucimobilis fp2001 with a 19.5% yield. the purified enzyme, which was homogeneous as shown by sds-page and isoelectric focusing, had a molecular weight of 112,000 and an isoelectric point of 7.1. the enzyme activity was accelerated by ca2+ and remained stable for several months when stored at -20 c. the optimum ph was 7.8; the optimum temperature was 45 degrees c. the km, v(max) and k(cat) for p-nitrophenyl alpha-l-rh ... | 2000 | 10648106 |
| evaluation of autoscan-w/a and the vitek gni+ automicrobic system for identification of non-glucose-fermenting gram-negative bacilli. | the autoscan-w/a (w/a; dade behring microscan inc., west sacramento, calif.) and vitek automicrobic system (vitek ams; biomérieux vitek systems, inc., hazelwood, mo.) are both fully automated microbiology systems. we evaluated the accuracy of these two systems in identifying nonglucose-fermenting gram-negative bacilli. we used the w/a with conventional-panel neg combo type 12 and vitek gni+ identification systems. a total of 301 isolates from 25 different species were tested. of these, 299 isola ... | 2000 | 10699007 |
| presence of two trans-o-hydroxybenzylidenepyruvate hydratase-aldolases in naphthalenesulfonate-assimilating sphingomonas paucimobilis ta-2: comparison of some properties. | two trans-o-hydroxybenzylidenepyruvate hydratase-aldolases named thbp ha a and thbp ha b were purified from a cell-free extract of naphthalenesulfonate-assimilating sphingomonas paucimobilis (formerly pseudomonas sp.) ta-2 to an electrophoretically homogeneous state by successive column chromatographies on deae-cellulose, deae-toyopearl 650m, sephacryl s-100, hydroxyapatite, and mono q. these enzymes were similar to each other in molecular mass (ca. 37 kda on sds-page, ca. 110 kda on ultracentri ... | 2000 | 10731665 |
| use of oligotrophic bacteria for the biological monitoring of heavy metals. | oligotrophic bacteria exhibited active growth even in nutritionally deficient medium made with nutrient broth that had been diluted with distilled water, 1 : 10 000. the oligotrophic bacteria, sphingomonas paucimobilis kps01 and burkholderia cepacia kpc01 and kpc02 were found to be highly susceptible to heavy metals and to be potentially useful as sensors for the assessment of toxicity. the susceptibility of the bacteria to metals was measured by incubating the bacteria with metals of varying co ... | 2000 | 10735254 |
| a novel sphingoglycolipid containing galacturonic acid and 2-hydroxy fatty acid in cellular lipids of sphingomonas yanoikuyae. | a novel sphingoglycolipid was isolated from sphingomonas yanoikuyae, and its structure was identified as a galacturonosyl-beta (1-->1)-ceramide. this was a characteristic sphingoglycolipid present in s. yanoikuyae and certain other species of sphingomonas, such as sphingomonas mali, sphingomonas terrae, and sphingomonas macrogoltabidus, but not in the type species of sphingomonas, sphingomonas paucimobilis. | 2000 | 10762275 |
| coexistence of two different o demethylation systems in lignin metabolism by sphingomonas paucimobilis syk-6: cloning and sequencing of the lignin biphenyl-specific o-demethylase (ligx) gene. | sphingomonas paucimobilis syk-6 can grow on several dimeric model compounds of lignin as a carbon and energy source. it has o demethylation systems on three kinds of substrates: 5, 5'-dehydrodivanillic acid (ddva), syringate, and vanillate. we previously reported the cloning of a gene involved in the tetrahydrofolate-dependent o demethylation of syringate and vanillate. in the study reported here, we cloned the gene responsible for ddva o demethylation. using nitrosoguanidine mutagenesis, a muta ... | 2000 | 10788391 |
| identification of the pgmg gene, encoding a bifunctional protein with phosphoglucomutase and phosphomannomutase activities, in the gellan gum-producing strain sphingomonas paucimobilis atcc 31461. | the pgmg gene of sphingomonas paucimobilis atcc 31461, the industrial gellan gum-producing strain, was cloned and sequenced. it encodes a 50,059-da polypeptide that has phosphoglucomutase (pgm) and phosphomannomutase (pmm) activities and is 37 to 59% identical to other bifunctional proteins with pgm and pmm activities from gram-negative species, including pseudomonas aeruginosa algc. purified pgmg protein showed a marked preference for glucose-1-phosphate (g1p); the catalytic efficiency was abou ... | 2000 | 10788412 |
| fatty acid-specific, regiospecific, and stereospecific hydroxylation by cytochrome p450 (cyp152b1) from sphingomonas paucimobilis: substrate structure required for alpha-hydroxylation. | fatty acid alpha-hydroxylase from sphingomonas paucimobilis is an unusual cytochrome p450 enzyme that hydroxylates the alpha-carbon of fatty acids in the presence of h2o2. herein, we describe our investigation concerning the utilization of various substrates and the optical configuration of the alpha-hydroxyl product using a recombinant form of this enzyme. this enzyme can metabolize saturated fatty acids with carbon chain lengths of more than 10. the km value for pentadecanoic acid (c15) was th ... | 2000 | 10858020 |
| ability of casamino acids to support gellan production by sphingomonas paucimobilis atcc 31461. | the ability of casamino acids and vitamin-assay casamino acids to support gellan production by sphingomonas paucimobilis atcc 31461 was examined in a medium containing glucose or corn syrup as the carbon source relative to yeast extract supplementation. when glucose or corn syrup served as the carbon source, the presence of yeast extract in the growth medium stimulated gellan production by strain atcc 31461 on casamino acids. using vitamin-assay casamino acids as the nitrogen source, the additio ... | 2000 | 10885499 |
| bacterial response to photocatalytic degradation of 6-chlorovanillin. | the oxidation of a 186 ppm 6-chlorovanillin solution was performed using impregnated tio2 glass rings in a 1 l photochemical reactor. fifty per cent degradation was obtained after 60 min with recirculation of the solution. then, oxidised samples were submitted under aerobic conditions to bacterial degradation in the pseudomonas paucimobilis (s37) and burkholderia cepacia (pzk). both selected aerobic bacteria degrade more efficiently the photocatalysed samples, being pzk strain better than s37. a ... | 2000 | 10901256 |
| unique heme environment at the putative distal region of hydrogen peroxide-dependent fatty acid alpha-hydroxylase from sphingomonas paucimobilis (peroxygenase p450(spalpha). | fatty acid alpha-hydroxylase from sphingomonas paucimobilis is a hydrogen peroxide-dependent cytochrome p450 (p450) enzyme (p450(spalpha)). in this study, heme-ligand exchange reactions of p450(spalpha) were investigated using the optical spectroscopic method and compared with those of various p450s. alkylamines (c >/= 5) induced changes in the spectrum of ferric p450(spalpha) to one typical of a nitrogenous ligand-bound low-spin form of ferric p450, although their affinities were lower than tho ... | 2000 | 10920253 |
| construction of mutants of sphingomonas paucimobilis defective in terminal mannose in the glycosphingolipid. | mutants of sphingomonaspaucimobilis defective in a part of the carbohydrate moiety of the glycosphingolipid (gsl) were constructed by transposon (tn5)-insertional mutagenesis. defective mutants were selected by elisa using the antibody recognizing the tetrasaccharide-type gsl (gsl-4a) of s. paucimobilis. eight defective mutants were selected from about 8,000 kanamycin-resistant strains, and seven of them were found to lack the terminal mannose of gsl-4a. the chemical structure of the mutant gsl ... | 2000 | 10923808 |
| enhanced attachment of bradyrhizobium japonicum to soybean through reduced root colonization of internally seedborne microorganisms. | internally seedborne microorganisms are those surviving common surface sterilization procedures. such microbes often colonize the radicle surface of a germinating soybean (glycine max) seed, introducing an undefined parameter into studies on attachment and infection by bradyrhizobium japonicum. bacterial isolates from surface-sterilized soybean seed, cv. williams 82 and cv. maverick, used in our studies, were identified as agrobacterium radiobacter, aeromonas sp., bacillus spp., chryseomonas lut ... | 2000 | 10932352 |
| biocatalytic preparation of a chiral synthon for a vasopeptidase inhibitor: enzymatic conversion of n(2)- | [4s-(4i,7i,10aj)]1-octahydro-5-oxo-4-[phenylmethoxy)carbonyl]amino]-7h-pyrido-[2,1-b] [1,3]thiazepine-7-carboxylic acid methyl ester (bms-199541-01) is a key chiral intermediate for the synthesis of omapatrilat (bms-186716), a new vasopeptidease inhibitor under development. by using a selective enrichment culture technique we have isolated a strain of sphingomonas paucimobilis sc 16113, which contains a novel l-lysine epsilon-aminotransferase. this enzyme catalyzed the oxidation of the epsilon-a ... | 2000 | 10938417 |
| [osteomyelitis caused by sphingomonas paucimobilis]. | 2000 | 10974775 | |
| enhanced degradation of hexachlorocyclohexane isomers by sphingomonas paucimobilis. | hexachlorocyclohexane (hch) has been banned for use in technologically advanced countries; however, it is still in use in tropical countries like india. earlier we reported the degradation of hch isomers by sphingomonas paucimobilis within 12 days of incubation. here we report the role of different factors that could enhance the degradation rate of hch isomers. we found that an increase in the cell number from 10(2) to 10(8) cells/ml resulted in an increased degradation rate of hch isomers viz. ... | 2000 | 11014865 |
| gellan gum. | for decades microbial exopolysaccharides have been invaluable ingredients in the food industry, as well as having many attractive pharmaceutical and chemical applications. gellan gum is a comparatively new gum elaborated by the gram-negative bacterium sphingomonas paucimobilis. although its physico-chemical properties have been well characterized, the ecology and physiology of sphingomonas, and the factors influencing the fermentation process for production of this gum have received much less at ... | 2000 | 11039329 |
| genetic and biochemical characterization of 4-carboxy-2-hydroxymuconate-6-semialdehyde dehydrogenase and its role in the protocatechuate 4,5-cleavage pathway in sphingomonas paucimobilis syk-6. | protocatechuate (pca) is the key intermediate metabolite in the lignin degradation pathway of sphingomonas paucimobilis syk-6 and is metabolized to pyruvate and oxaloacetate via the pca 4,5-cleavage pathway. we characterized the 4-carboxy-2-hydroxymuconate-6-semialdehyde (chms) dehydrogenase gene (ligc). chms is the 4,5-cleavage product of pca and is converted into 2-pyrone-4,6-dicarboxylate (pdc) by ligc. we found that ligc was located 295 bp downstream of ligb, which encodes the large subunit ... | 2000 | 11073908 |
| crystal structure of the haloalkane dehalogenase from sphingomonas paucimobilis ut26. | the haloalkane dehalogenase from sphingomonas paucimobilis ut26 (linb) is the enzyme involved in the degradation of the important environmental pollutant gamma-hexachlorocyclohexane. the enzyme hydrolyzes a broad range of halogenated cyclic and aliphatic compounds. here, we present the 1.58 a crystal structure of linb and the 2.0 a structure of linb with 1,3-propanediol, a product of debromination of 1,3-dibromopropane, in the active site of the enzyme. the enzyme belongs to the alpha/beta hydro ... | 2000 | 11087355 |
| the 4-oxalomesaconate hydratase gene, involved in the protocatechuate 4,5-cleavage pathway, is essential to vanillate and syringate degradation in sphingomonas paucimobilis syk-6. | sphingomonas paucimobilis syk-6 is able to grow on various dimeric lignin compounds, which are converted to vanillate and syringate by the actions of unique lignin degradation enzymes in this strain. vanillate and syringate are degraded by the o-demethylase and converted into protocatechuate (pca) and 3-o-methylgallate (3mga), respectively. pca is further degraded via the pca 4,5-cleavage pathway, while the results suggested that 3mga is degraded through another pathway in which pca 4,5-dioxygen ... | 2000 | 11092855 |
| reaction mechanism and stereochemistry of gamma-hexachlorocyclohexane dehydrochlorinase lina. | gamma-hexachlorocyclohexane dehydrochlorinase (lina) catalyzes the initial steps in the biotransformation of the important insecticide gamma-hexachlorocyclohexane (gamma-hch) by the soil bacterium sphingomonas paucimobilis ut26. stereochemical analysis of the reaction products formed during conversion of gamma-hch by lina was investigated by gc-ms, nmr, cd, and molecular modeling. the nmr spectra of 1,3,4,5,6-pentachlorocyclohexene (pcch) produced from gamma-hch using either enzymatic dehydrochl ... | 2001 | 11099497 |
| biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants. | the ph indicator dye-based colorimetric method and multivariate experimental design were used for the systematic biochemical characterization of the broad-specificity enzymes haloalkane dehalogenases. halogenated compounds for characterization of the enzymes were selected using principal component analysis. the substrates were characterised by 24 physico-chemical and structural descriptors. thirty-four substrates were selected for testing out of 194 halogenated compounds. relative activities det ... | 2001 | 11165344 |
| a simple and easy method for the monitoring of environmental pollutants using oligotrophic bacteria. | sphingomonas paucimobilis kps01, an oligotrophic bacterium isolated from soil, may be a useful tool for monitoring heavy metals. previous methods relying on counting of viable cells require a relatively long time and some skill; we have developed a method based on optical density (o.d.) measurements which is significantly faster and does not require skilled personnel. the results of the o.d. and viable count methods were consistent; both methods detected heavy metals at concentrations ranging fr ... | 2001 | 11169034 |
| analysis of endophytic bacterial communities of potato by plating and denaturing gradient gel electrophoresis (dgge) of 16s rdna based pcr fragments. | the diversity of endophytic bacterial populations of potato (solanum tuberosum cv desirée) was assessed using a combination of dilution plating of plant macerates followed by isolation and characterization of isolates, and direct pcr-dgge on the basis of dna extracted from plants. the culturable endophytic bacterial communities detected in potato stem bases as well as in roots were in most cases on the order 103 to 105 cfu g?1 of fresh plant tissue. dilution plating revealed that a range of bact ... | 2001 | 12032611 |
| biodegradation of sulfanilic acid by pseudomonas paucimobilis. | an aerobic bacterium, isolated from a contaminated site, was able to degrade sulfanilic acid (4-aminobenzenesulfonic acid) and was identified as pseudomonas paucimobilis. the isolate could grow on sulfanilic acid (sa) as its sole carbon and nitrogen source and metabolized the target compound to biomass. the bioconversion capacity depended on the sulfanilic acid concentration; greater than 98% elimination of the hazardous compound was achieved at low (10 mm) sulfanilic acid concentration, and the ... | 2001 | 11234949 |
| a water-soluble homodimeric serine palmitoyltransferase from sphingomonas paucimobilis ey2395t strain. purification, characterization, cloning, and overproduction. | serine palmitoyltransferase (spt, ec ) is a key enzyme in sphingolipid biosynthesis and catalyzes the decarboxylative condensation of l-serine and palmitoyl-coenzyme a to 3-ketodihydrosphingosine. we found that the gram-negative obligatory aerobic bacteria sphingomonas paucimobilis ey2395(t) have significant spt activity and purified spt to homogeneity. this enzyme is a water-soluble homodimeric protein unlike eukaryotic enzymes, known as heterodimers composed of tightly membrane-bound subunits, ... | 2001 | 11279212 |
| proposal of sphingomonas wittichii sp. nov. for strain rw1t, known as a dibenzo-p-dioxin metabolizer. | a polyphasic taxonomic study was performed on sphingomonas sp. strain rw1t. the organism was isolated from water of the river elbe and has been known as a potent metabolizer of dibenzo-p-dioxin and its relatives. tlc of a mild alkaline hydrolysate of extractable cellular lipids of strain rw1t and type strains of 21 sphingomonas species gave a spot of sphingoglycolipid (sgl)-1 (glucuronosyl ceramide), which is characteristic of sphingomonads. in addition, strain rw1t and type strains of three sph ... | 2001 | 11321072 |
| enzymatic synthesis of chiral intermediates for omapatrilat, an antihypertensive drug. | biocatalytic processes were used to prepare chiral intermediates required for the synthesis of omapatrilat 1 by three different routes. the synthesis and enzymatic conversion of 2-keto-6-hydroxyhexanoic acid 3 to l-6-hydroxynorleucine 2 was demonstrated by reductive amination using beef liver glutamate dehydrogenase. to avoid the lengthy chemical synthesis of the ketoacid 3, a second route was developed to prepare the ketoacid by treatment of racemic 6-hydroxy norleucine [readily available from ... | 2001 | 11337276 |
| structural studies of s-7, another exocellular polysaccharide containing 2-deoxy-arabino-hexuronic acid. | the exocellular polysaccharide s-7, a heteropolysaccharide from azotobacter indicus var. myxogenes has been studied using methylation analysis, smith degradation, partial acid hydrolysis, nmr spectroscopy and mass spectrometry as the principal methods. it is concluded that the repeating unit has the following structure: [structure: see text] the absolute configuration of the deoxyhexuronic acid was deduced from 1h nmr chemical shifts and is most likely d. approximately two o-acetyl groups per re ... | 2001 | 11383898 |