Publications

nematophagous fungi from decomposing cattle faeces in argentina.	biological control of gastrointestinal nematodes of ruminants by use of nematophagous fungi would become part of any livestock parasite integral control system. identifying autochthonous species that could then be selected for mass production is an important phase in the practical use of biological control.	2015	25766793
high trap formation and low metabolite production by disruption of the polyketide synthase gene involved in the biosynthesis of arthrosporols from nematode-trapping fungus arthrobotrys oligospora.	a group of morphology regulatory arthrosporol metabolites have been recently characterized from carnivorous fungus arthrobotrys oligospora that can develop trapping networks to capture their prey. a combination of genetic manipulation and chemical analyses was applied to characterize the function of one polyketide synthase (pks) gene aol_s00215g283 in a. oligospora, which was putatively involved in the production of 6-methylsalicylic acid. high-performance liquid chromatography analysis showed t ...	2015	26422178
a proposed adhesin aomad1 helps nematode-trapping fungus arthrobotrys oligospora recognizing host signals for life-style switching.	the nematode-trapping fungus arthrobotrys oligospora is an important natural enemy of nematodes. it can capture nematodes by producing a special mycelial structure called adhesive network or trap. the trap is also a signature of the fungus switching from the saprophytic lifestyle to the predacious lifestyle. at present, little is known about the mechanism of lifestyle switch in nematode-trapping fungi. here we describe the effect of a cell wall protein called aomad1 on lifestyle switch. the disr ...	2015	25724687
expression of serine proteinase p186 of arthrobotrys oligospora and analysis of its nematode-degrading activity.	the nematode-trapping fungi possess a unique capability of predating and invading nematodes. as a representative nematode-trapping fungus, arthrobotrys oligospora has been widely used to study the interactions between nematode-trapping fungi and their hosts. serine proteinase is one of the important virulence factors during process of invasion of the nematode-trapping fungi into nematodes. in this study, using reverse transcription polymerase chain reaction, we amplified the gene sequence of ser ...	2015	26419902
screening of different sample types associated with sheep and cattle for the presence of nematophagous fungi in china.	a total of 1502 samples, including feces of sheep (793) and cattle (348), pasture soil (118), dung compost (147) and barn soil (96), were examined between october 2012 and august 2014 to discover potential strains of nematophagous fungi for the biological control of livestock-parasitic nematodes. these samples were collected from 87 sites located in 48 counties of 20 provinces (autonomous regions/municipalities) of china. fungi were identified down to a species level. four hundred and seventy-se ...	2016	26344826
gph1 is involved in glycerol accumulation in the three-dimensional networks of the nematode-trapping fungus arthrobotrys oligospora.	turgor is very important for the invasive growth of fungal pathogens. glycerol, a highly osmotic solvent, is considered to play an important role in turgor generation. the nematophagous fungus arthrobotrys oligospora mainly lives as a saprophyte. in the presence of nematodes, a. oligospora enters the parasitic stage by forming three-dimensional networks (traps) to capture nematodes. in a. oligospora, we found that glycerol accumulated during nematode-induced trap formation. we demonstrated that ...	2016	27796934
nematicidal key precursors for the biosynthesis of morphological regulatory arthrosporols in nematode-trapping fungus arthrobotrys oligospora.	arthrobotrys oligospora is the first recognized nematode-trapping fungus and by far the most abundant in the environment. our recent study revealed the polyketide synthase (pks) gene aol_s00215g283 in a. oligospora involved in the production of many secondary metabolites and the trap formation of the fungus. here we report that the disruption of two genes in the upstream flanking region of the gene aol_s00215g283, aol_s00215g281 and aol_s00215g282, which putatively encoded one amidohydrolase and ...	2016	27723963
in vitro susceptibility of nematophagous fungi to antiparasitic drugs: interactions and implications for biological control.	the fast anthelmintic resistance development has shown a limited efficiency in the control of animal's endoparasitosis and has promoted research using alternative control methods. the use of chemicals in animal anthelmintic treatment, in association with nematophagous fungi used for biological control, is a strategy that has proven to be effective in reducing the nematode population density in farm animals. this study aims to verify the in vitro susceptibility of the nematophagous fungi arthrobo ...	2016	27706395
the nitrate assimilation pathway is involved in the trap formation of arthrobotrys oligospora, a nematode-trapping fungus.	nutrient conditions are important for trap formation and a switch from saprophytic to predacious lifestyle in nematode-trapping fungi. arthrobotrys oligospora is a typical nematode-trapping fungus which can capture and kill nematodes by means of traps formed in response to the presence of their prey. a nutrient-limited environment was previously shown to be important for trap formation, which was induced by several inferior nitrogen sources, such as sodium nitrate, in the absence of the cell wal ...	2016	27174557
nematode-trapping fungi and fungus-associated bacteria interactions: the role of bacterial diketopiperazines and biofilms on arthrobotrys oligospora surface in hyphal morphogenesis.	in soil, nematode-trapping fungi and bacteria often share microhabitats and interact with each other, but effects of fungus-associated bacteria on its trap formation are underestimated. we have ascertained the presence of stenotrophomonas and rhizobium genera associated with a. oligospora gj-1. after a. oligospora gj-1 without associated bacteria (cured arthrobotrys) was co-cultivated with stenotrophomonas and its supernatant extract, microscopic study of hyphae from co-cultivation indicated tha ...	2016	27130625
phylogenic analysis of adhesion related genes mad1 revealed a positive selection for the evolution of trapping devices of nematode-trapping fungi.	adhesions, the major components of the extracellular fibrillar polymers which accumulate on the outer surface of adhesive traps of nematode-trapping fungi, are thought to have played important roles during the evolution of trapping devices. phylogenetic analyses based on the genes related to adhesive materials can be of great importance for understanding the evolution of trapping devices. recently, aomad1, one homologous gene of the entomopathogenic fungus metarhizium anisopliae cell wall protei ...	2016	26941065
induction of trap formation in nematode-trapping fungi by bacteria-released ammonia.	a total of 11 bacterial strains were assayed for bacteria-induced trap formation in the nematode-trapping fungus arthrobotrys oligospora ymf1·01883 with two-compartmented petri dish. these strains were identified on the basis of their 16s rrna gene sequences. volatile organic compounds (vocs) of eight isolates were extracted using solid-phase micro-extraction (spme) and their structures were identified based on gas chromatography-mass spectrometry (gc-ms). at the same time, all isolates were use ...	2016	26928264
nematophagous fungus arthrobotrys oligospora mimics olfactory cues of sex and food to lure its nematode prey.	to study the molecular basis for predator-prey coevolution, we investigated how caenorhabditis elegans responds to the predatory fungus arthrobotrys oligospora. c. elegans and other nematodes were attracted to volatile compounds produced by a. oligospora. gas-chromatographic mass-spectral analyses of a. oligospora-derived volatile metabolites identified several odors mimicking food cues attractive to nematodes. one compound, methyl 3-methyl-2-butenoate (mmb) additionally triggered strong sex- an ...	2017	28098555
the woronin body in the nematophagous fungus arthrobotrys oligospora is essential for trap formation and efficient pathogenesis.	the woronin body is a unique organelle in pezizomycotina species. following the injury of hyphae, it can quickly seal the septal pores to reduce the loss of cytoplasm and promote hyphal healing. the woronin body is also considered a significant factor in efficient pathogenesis in many fungal pathogens. in this study, we identified aohex1, a homologue of neurospora crassa hex1, a main component of the woronin body in the genome of the nematode-trapping fungus arthrobotrys oligospora. to study the ...	2017	28007213