Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| the synthesis and enzymatic polymerization of nucleotides containing mercury: potential tools for nucleic acid sequencing and structural analysis. | a simple acetoxymercuration reaction for introducing covalently bound mercury atoms into nucleotides is described. the 5-mercuriacetate derivatives of utp, ctp, dutp, and dctp, as well as the 7-mercuriacetate derivative of 7-deazaatp, have been prepared by this procedure and tested as substrates for nucleic acid polymerases. these nucleotides, in the absence of added mercaptan, are not polymerized and in most instances are potent enzyme inhibitors. however, conversion of these mercuriacetates to ... | 1973 | 4365367 |
| avian myeloblastosis virus dna polymerase: initiation of dna synthesis and an associated ribonuclease. | 1973 | 4369800 | |
| ribonuclease h activity present in purified dna polymerase from avian myeloblastosis virus. | 1973 | 4349324 | |
| structural studies on avian myeloblastosis virus: conditions for isolation and biochemical characteristics of the core component. | 1973 | 4349411 | |
| aminoacyl-trna synthetase activity in virions of avian myeloblastosis virus. | 1973 | 4350587 | |
| quantitative determination and location of newly synthesized virus-specific ribonucleic acid in chicken cells infected with rous sarcoma virus. | a sensitive and quantitative nucleic acid hybridization assay for the detection of radioactively labeled avian tumor virus-specific rna in infected chicken cells has been developed. in our experiments we made use of the fact that dna synthesized by virions of avian myeloblastosis virus in the presence of actinomycin d (amv dna) is complementary to at least 35% of the sequences of 70s rna from the schmidt-ruppin strain (srv) of rous sarcoma virus. annealing of radioactive rna (either srv rna or r ... | 1973 | 4350719 |
| [high molecular weight rapidly labelled ribonucleic acid (rna) and virus production in chick fibroblasts infected with avian myeloblastosis virus]. | 1973 | 4350820 | |
| lack of sequence homology between the 70s rna of various rna tumor viruses and the dna of simian virus 40 or polyoma virus. | no significant hybridization was detected of dna from simian virus 40 or polyoma virus, and of 70s rna from avian myeloblastosis virus, murine leukemia virus (rauscher), murine sarcoma virus (kirsten), rd-114b, simian sarcoma virus-1, or mason-pfizer virus. | 1973 | 4351455 |
| minor base composition of "70s-associated" 4s rna from avian myeloblastosis virus. | using a chemical isotope derivative method, we have determined the minor base composition of the "70s-associated" 4s rna isolated from avian myeloblastosis virus. the minor base composition of this "70s-associated" 4s rna is strikingly similar to that of the corresponding "free" 4s rna of the virion. this minor base content, plus the capacity to esterify amino acids, establishes that both of these virion 4s rna fractions contain transfer rna. the percentage of minor bases in virion "70s-associat ... | 1973 | 4351808 |
| amino-acid acceptor activity of the "70s-associated" 4s rna from avian myeloblastosis virus. | the "70s-associated" 4s rna from avian myeloblastosis virus contains transfer rna, as determined by aminoacylation. the various amino acids esterified, plus the great number of minor bases found in this "70s-associated" 4s rna indicate that a sizeable fraction, but not all, of this rna is transfer rna. | 1973 | 4352225 |
| the inhibition of the avian myeloblastosis virus dna polymerase by poly(u) fractions of varying chain length. | 1973 | 4352244 | |
| spontaneous regression of leukemia in chickens infected with avian myeloblastosis virus. | 1973 | 4352529 | |
| identification of a large polypeptide precursor of avian oncornavirus proteins. | antibody to partially disrupted avian myeloblastosis virus was used to selectively precipitate newly synthesized intracellular viral polypeptides from extracts of infected chicken cells. when analyzed by sodium dodecyl sulfate-gel electrophoresis, immune precipitates from extracts of cells pulse-labeled for 10 min with [(35)s]methionine contain none of the major virion polypeptides. instead they show prominent viral specific polypeptides of molecular weight 76,000 and 12,000, as well as minor qu ... | 1973 | 4352653 |
| [resistance of german chicken lines against avian myeloblastosis virus (amv)]. | 1973 | 4354748 | |
| subunits of oncornavirus high-molecular-weight rna. i. stepwise conversion of 60 s amv (avian myeloblastosis virus) rna to subunits. | 1973 | 4354929 | |
| methionine transfer ribonucleic acids of avian myeloblastosis virus. | chick-embryo cells contain four isoaccepting species of methionine transfer rna (i-iv). one species (i) is the initiator, trna(f) (met), and the others (ii, iii, and iv) are the donors of internal methionyl residues (trna(m) (met)). over 85% of the trna(met) in purified avian myeloblastosis virus consists of one trna(m) (met) species, which resembles host-cell trna(met) iv with respect to chromatographic properties on rpc-5, electrophoretic mobility of the terminal methionyl-oligonucleotide, and ... | 1973 | 4355368 |
| chromatographic analyses of isoaccepting trnas from avian myeloblastosis virus. | avian myeloblastosis virus (amv) 4s rna was tested for amino acid acceptor activity for 18 of the 20 amino acids. a nonrandom distribution of viral trnas was found compared with trna from normal liver or from amv-infected leukemic myeloblasts, confirming previous reports. methionine and proline trnas were considerably enriched, whereas glutamic acid, glutamine, serine, tyrosine, and valine trnas were markedly depleted in amv relative to homologous cellular trnas. the seven amv trnas with the gre ... | 1973 | 4355849 |
| separation of dna sequences complementary to the rna of avian myeloblastosis virus from chicken dna by alkaline cesium chloride density sedimentation. | density gradient sedimentation in alkaline cesium chloride of dna from normal chicken embryos or leukemic myeloblasts fragmented to a size of 13s revealed that the dna sequences complementary to 70s avian myeloblastosis virus rna sedimented in the high guanine plus cytosine region ahead of the main peak of cellular dna. when the dna was fragmented into pieces of 6.6s there was a broader distribution of the dna sequences complementary to the viral rna. this technique could be employed as a step t ... | 1973 | 4355853 |
| structural studies of avian myeloblastosis virus: presence of transfer rna in core component. | 1973 | 4355967 | |
| subunits of oncornavirus high-molecular-weight rna. ii. detection of double-stranded-regions in 60s amv (avian myeloblastosis virus) rna. | 1973 | 4356817 | |
| rna-dna covalent bonds between the rna primers and the dna products formed by rna tumor virus dna polymerase. | initiation of dna synthesis by endogenous rna primer molecules was studied with three different rna tumor viruses. the influence of the method of virus disruption on the observed rna-dna bonds was ascertained. ether disrupted virions of both murine leukemia virus (mulv) and the b77 strain of avian sarcoma virus (b77 virus) have rc-dc and ra-da covalent linkages between rna primers and newly synthesized dna. none of the 14 other possible bonds were formed. ether-disrupted virions of avian myelobl ... | 1973 | 4357517 |
| outer membrane of avian myeloblastosis virus. | guinea pigs immunized intracerebrally with avian myeloblastosis virus (amv) produced antiserum which reacted with intact virus particles in complement fixation. the antigen in question appeared to be located on the surface of the virion and could be distinguished from the type-specific virus envelope and the group-specific internal antigens of chicken leukosis-sarcoma viruses (chilsv). the material could be isolated by sequential treatments of amv with bromelin, tween 20, and freeze-thawing, and ... | 1973 | 4128385 |
| studies on rat c-type virus "werc". i. presence of gs-interspecies antigen and a new complement fixing antigen, shared by avian myeloblastosis virus. | 1973 | 4129767 | |
| synthesis of (3h)dna complementary to ovalbumin messenger rna: evidence for limited copies of the ovalbumin gene in chick oviduct. | accumulation of ovalbumin messenger rna in chick oviduct is absolutely dependent upon estrogen. after estrogen treatment, ovalbumin comprises 60-65% of the total oviduct protein. we used maximally stimulated animals to extract and partially purify the ovalbumin messenger rna. the final product was enriched about 100-fold in activity with respect to this specific messenger rna. this ovalbumin messenger rna fraction was used to direct the synthesis of a complementary [(3)h]dna in the presence of r ... | 1973 | 4129926 |
| structural components of avian myeloblastosis virus. | 1973 | 4130396 | |
| a single subunit from avian myeloblastosis virus with both rna-directed dna polymerase and ribonuclease h activity. | two structurally distinct forms of rna-directed dna polymerase from avian myeloblastosis virus were resolved by chromatography on phosphocellulose and purified. in addition to rna-directed dna polymerase activity, both enzymes had ribonuclease h (rnase h) activity, which degraded the rna moiety of rna.dna hybrids. as determined by sodium dodecyl sulfate-polyacrylamide disc-gel electrophoresis, one form had two subunits, alpha (alpha) and beta (beta), with molecular weights of 65,000 and 105,000, ... | 1973 | 4119223 |
| mechanism of action of ribonuclease h isolated from avian myeloblastosis virus and escherichia coli. | purified preparations of rna-dependent dna polymerase isolated from avain myeloblastosis virus contain rnase h activity. labeled ribohomopolymers are degraded in the presence of their complementary deoxyribopolymer, except [(3)h]poly(u).poly(da). the degradation products formed from [(3)h]poly(a).poly(dt) were identified as oligonucleotides containing 3'-hydroxyl and 5'-phosphate termini, while amp was not detected. the nuclease has been characterized as a processive exonuclease that requires en ... | 1973 | 4119789 |
| inhibition of dna polymerase of avian myeloblastosis virus by an alkaloid extract from narcissus tazetta l. | 1973 | 4123212 | |
| enzymatic synthesis of dna complementary to purified 14s messenger rna of immunoglobulin light chain. | the 14s messenger rna, which contains poly(adenylic acid), of mopc 41 (mouse plasmocytoma) immunoglobulin light chain, purified to a single peak as shown by polyacrylamide gel electrophoresis, was used to synthesize complementary dna with the rna-dependent dna polymerase of avian myeloblastosis virus. dna synthesis is entirely dependent on added rna template and oligo(dt) primer. both the size and the concentration of the primer affect the reaction. the product behaves similarly to dna during ce ... | 1973 | 4123688 |
| demonstration of globin messenger sequences in giant nuclear precursors of messenger rna of avian erythroblasts. | highly purified globin mrna from ducks was copied with rna-directed dna polymerase from avian myeloblastosis virus into anti-messenger dna. with excess rna, more than 90% of this dna annealed back to its template with a c(o)t/2 value of 7.5 x 10(-4) mol.sec. liter(-1); the melting temperature of the hybrid was 86 degrees . giant nuclear rna fractions with sedimentation coefficients of more than 50 s formed hybrids of almost equal stability at c(o)t/2 values of 0.05-0.42 mol.sec. liter(-1), indic ... | 1973 | 4123930 |
| primer requirement and template specificity of a dna polymerase of chick embryo. | a dna polymerase purified from extracts of chick embryo catalyzes the synthesis of poly(dt) in the presence of poly(ra) or poly(da) as template and poly(dt) or oligo(dt) as primer. oligo- or polyribonucleotides are relatively ineffective initiators of polydeoxynucleotide polymerization. using activated dna as template, the enzyme catalyzes a repair-like reaction that resembles that catalyzed by rna-directed dna polymerase of rna tumor viruses and escherichia coli dna polymerase ii. with natural ... | 1973 | 4123933 |
| quantitative deficiency of chain-specific globin messenger ribonucleic acids in the thalassemia syndromes. | a hybridization assay procedure was devised that makes possible quantitation of the ratio of mrna of alpha to mrna of beta globin chains in an rna sample. the assay uses the radioactive synthetic dna copies obtained by incubation of rna-dependent dna polymerase of avian myeloblastosis virus with rabbit globin mrna that is 80-90% enriched in mrna specific for synthesis of alpha or beta globin chains. the rabbit alpha-chain mrna is obtained from the postribosomal supernatant of rabbit reticulocyte ... | 1973 | 4124305 |
| [identification of a "factor" of deoxyribonuclease in reactions with the reverse transcriptase of amv]. | 1973 | 4126216 | |
| lack of serological relationship among dna polymerases of avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and chicken cells. | antibodies against a large and a small dna polymerase isolated from chicken embryos and against avian myeloblastosis virus dna polymerase were used to study the serological relationships of the dna polymerase activities of three avian systems with rna and a dna polymerase-avian leukosis-sarcoma viruses, reticuloendotheliosis viruses, and a fraction from uninfected chicken cells. the dna polymerase activity of disrupted virions of all avian leukosis-sarcoma viruses tested was neutralized to the s ... | 1973 | 4127028 |
| inhibition of rna-dependent dna polymerase of avian myeloblastosis virus by pyran copolymer. | pyran copolymer, a known immunostimulator, was found to be a potent inhibitor of purified dna polymerase (deoxynucleosidetriphosphate: dna deoxynucleotidyltransferase; ec 2.7.7.7) isolated from avian myeloblastosis virus. unlike other inhibitors, pyran showed unique features of inhibition. it interacts with the polymerase at a region other than the template site. the inhibitory effect was overcome only by excess enzyme and not affected by excess template. the degree of inhibition was not templat ... | 1974 | 4131275 |
| hamster leukemia virus: lack of endogenous dna synthesis and unique structure of its dna polymerase. | infectious hamster leukemia virus (halv) contains a dna polymerase different from those of murine and avian viruses. no endogenous reaction directed by the 60 to 70s rna of halv could be demonstrated in detergenttreated halv virions, nor could the purified dna polymerase copy added viral rna. the virion rna could, however, act as template for added avian myeloblastosis virus dna polymerase and the halv dna polymerase could efficiently utilize homopolymers as templates. the halv enzyme was like o ... | 1974 | 4132918 |
| reverse transcriptase from avian myeloblastosis virus: a zinc metalloenzyme. | 1974 | 4133876 | |
| separation of ribonuclease h and rna directed dna polymerase (reverse transcriptase) of murine type-c rna tumor viruses. | ribonuclease h (rna.dna-hybrid ribonucleotidohydrolase, ec 3.1.4.34) has been reported to copurify with reverse transcriptase (rna directed dna polymerase) of rna tumor viruses. in addition, viral specific ribonuclease h and reverse transcriptase of avian type-c viruses are thought to be part of the same polypeptide. in this report we show that a fraction of the ribonuclease h activity from rauscher murine leukemia and kirsten murine sarcoma viruses was separated from reverse transcriptase by an ... | 1974 | 4134616 |
| rna-dependent dna polymerase (reverse transcriptase) from avian myeloblastosis virus: a zinc metalloenzyme. | rna tumor viruses contain a characteristic rna-dependent dna polymerase (reverse transcriptase) which has been thought to be related to the induction of leukemia by this virus. a disturbance in a zinc-dependent enzyme system was first postulated to account for the demonstrated differences in zinc metabolism of normal and leukemic leukocytes [vallee et al. in (1949) acta unio. int. contra cancrum 6, 869 and (1950) acta unio. int. contra cancrum 6, 1102]. in order to investigate the relationship b ... | 1974 | 4134617 |
| transcription of heteropolymeric regions of avian myeloblastosis virus high molecular weight rna with escherichia coli dna polymerase i. | 1974 | 4135407 | |
| nucleotide sequences of human globin messenger rna. | globin messenger rna, isolated from human peripheral blood reticulocytes, was transcribed into complementary dna by use of the rna-dependent dna polymerase of avian myeloblastosis virus. the complementary dna was then transcribed into (32)p-labeled complementary rna by e. coli rna polymerase in the presence of alpha-(32)p-labeled ribonucleoside triphosphates. the fingerprint pattern obtained from ribonuclease t1 digests of human globin complementary rna was specific and reproducible. different p ... | 1974 | 4135409 |
| different mode of action of ribonuclease h in purified alpha and alpha beta ribonucleic acid-directed deoxyribonucleic acid polymerase from avian myeloblastosis virus. | 1974 | 4136633 | |
| purification of the rna-directed dna polymerase from avian myeloblastosis virus and its assay with polynucleotide templates. | 1974 | 4137148 | |
| rna-dependent dna polymerase from avian myeloblastosis virus. | 1974 | 4137175 | |
| the infidelity of avian myeloblastosis virus deoxyribonucleic acid polymerase in polynucleotide replication. | 1974 | 4137275 | |
| comparative biochemical properties of rna-directed dna polymerases from rauscher murine leukemia virus and avian myeloblastosis virus. | 1974 | 4137585 | |
| decreased globin messenger rna in thalassemia by hydridization and biologic activity assays. | 1974 | 4137600 | |
| purification of avian myeloblastosis virus dna polymerase by affinity chromatography on polycytidylate-agarose. | polycytidylic acid [poly(rc)] covalently linked to cyanogen bromide-activated agarose is an effective affinity matrix for the rna-dependent dna polymerase from avian myeloblastosis virus. poly(rc)-agarose is capable of binding large quantities of avian myeloblastosis dna polymerase, which is then eluted by using a linear kcl gradient of increasing concentration. the dna polymerase isolated from crude, detergent-disrupted virions by a single pass through columns of poly(rc)-agarose appears nearly ... | 1974 | 4138457 |
| the length of the terminal repetition in adenovirus-2 dna. | adenovirus-2 dna was end-labeled by partial digestion with escherichia coli exonuclease iii and resynthesis with the dna polymerase from avian myeloblastosis virus and alpha-(32)p-labeled deoxyribonucleoside triphosphates. this end-labeled dna was cleaved with several specific endonucleases and the terminal fragments were characterized by gel electrophoresis and pyrimidine tract analysis. two endonucleases gave identical fragments from both ends, presumably from cleavage within the inverted term ... | 1974 | 4139703 |
| enzymatic synthesis of solid phase-bound dna sequences corresponding to specific mammalian genes. | with oligo(dt)-cellulose as primer, rna-dependent dna polymerase catalyzes the synthesis of cellulose-bound dna that is complementary to mouse globin mrna. the resulting cellulose-bound or solid phase complementary dna hybridizes specifically with globin mrna and permits the recovery of intact globin mrna. this simple technique for the synthesis of solid phase-bound complementary dna provides an additional and convenient method for the purification of specific genetic sequences. | 1974 | 4139705 |
| use of globin cdna as a hybridization probe for globin mrna. | 1974 | 4139921 | |
| mechanism of action of ribonucleic acid-directed deoxyribonucleic acid polymerase. i. transcription of globin messenger ribonucleic acid. | 1974 | 4140187 | |
| reverse transcriptase: correlation of zinc content with activity. | evidence is presented that dna polymerase of avian myeloblastosis virus has an obligatory zinc requirement for activity. previous studies indicate that the purified polymerase contains zinc in a stoichiometry of about 1 g-atom/mole. we now find that the enzyme-bound zinc is exchangeable with radioactive (65)zn; after isoelectric focusing, the radioactive (65)zn is coincident with polymerase activity. dialysis of the (65)zn-labeled polymerase against the chelator, 1,10-phenanthroline, results in ... | 1974 | 4140513 |
| studies on the reverse transcriptase of rna tumor viruses. structural relatedness of two subunits of avian rna tumor viruses. | the structural relationship of the small (alpha) and large (beta) subunits of reverse transcriptase isolated from two avian rna tumor viruses has been examined by tryptic peptide analysis. comparison of the tryptic hydrolysates of the isolated subunits by two-dimensional separation on thin-layer cellulose plates indicates that (i) the alpha subunit of reverse transcriptase of avian myeloblastosis virus is structurally related to the beta subunit; (ii) the alpha and beta subunits of the enzyme of ... | 1974 | 4140514 |
| structural components of rna tumor viruses. | 1974 | 4140684 | |
| biochemical evidence for the presence of rna tumor viruses in human leukemic plasma. | 1974 | 4141320 | |
| reverse transcriptase of an oncornavirus-like particle derived from human hela cells. | 1974 | 4141324 | |
| characterization of free reverse transcriptase in the blood plasma of leukemic chicken. | 1974 | 4142720 | |
| ribonuclease activity tightly bound to ribosome-like structures isolated from avian myeloblastosis virus. | 1974 | 4156155 | |
| detection of naturally occurring antibodies to rna-dependent dna polymerase of murine leukemia virus in kidney eluates of akr mice. | specific antibodies to the rna-dependent dna polymerase (reverse transcriptase) of murine type c viruses have been isolated from the renal glomeruli of both leukemic and nonleukemic akr mice where they presumably had been deposited as immune complexes. the antibodies were shown to have sedimentation coefficients of 26s to 28s and 5s to 7s on sucrose rate zonal centrifugation. inactivation with monospecific antisera to various mouse immunoglobulins identified antibodies as being in both immunoglo ... | 1974 | 4129792 |
| structural studies of avian myeloblastosis virus: comparison of polypeptides in virion and core component by dodecyl sulfate-polyacrylamide gel electrophoresis. | two different systems of dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) in separate laboratories detected analogous patterns of dye bands in virions of avian myeloblastosis virus (amv). at least 11 of the dye bands co-migrated with the major polypeptides reported in rous sarcoma virus. particles with the morphology of the amv core component, obtained after exposure of amv to the nonionic surfactant sterox sl, contained major polypeptides p12, p27, p60, p64, p91, and p98. the polyp ... | 1974 | 4129794 |
| dna polymerase in virions of a reptilian type c virus. | a study was made of the dna polymerase of reptilian type c virus isolated from russell's viper spleen cells. simultaneous detection experiments demonstrated the presence of 70s rna and rna-dependent dna polymerase activity in reptilian type c virions. the endogenous activity was dependent on the addition of all four deoxynucleotide triphosphates and demonstrated an absolute requirement for a divalent cation. the reptilian viral dna polymerase elutes from phosphocellulose at 0.22 m salt. in this ... | 1974 | 4129837 |
| interaction of purified ribonucleic acid directed deoxyribonucleic acid polymerase of avian myeloblastosis virus and murine sarcoma-leukemia virus with a rifamycin sv derivative. | 1974 | 4129863 | |
| enzymatic radioiodination of the envelope proteins of avian myeloblastosis virus. | purified avian myeloblastosis virus was iodinated by the lactoperoxidase method. disruption of the labeled virions and chromatography of the viral proteins showed that radioiodine was associated only with the glycoproteins of the viral envelope. reaction of the radiolabeled virus with antiviral antibody followed by mild detergent treatment and subsequent recovery of immune complexes showed this technique to be useful for the isolation of viral envelope antigens. | 1974 | 4359430 |
| differences between the integration of avian myeloblastosis virus dna in leukemic cells and of endogenous viral dna in normal chicken cells. | the nature of integrated viral dna in normal and leukemic chicken cells has been studied by sequential nucleic acid hybridization procedures that localize the viral specific dna in cellular dna regions differing in reiteration frequency. first, dna.dna reassociation was employed to fractionate cellular dna sequences according to their reiteration frequencies. next, the dna in each fraction was denatured, immobilized on nitrocellulose filters, and then hybridized with viral [(3)h]rna. in normal c ... | 1974 | 4369920 |
| isolation of infectious dna from avian myeloblastosis virus transformed leukemic cells. | 1974 | 4370134 | |
| response of chick-embryo kidney cells in vitro to two avian tumor viruses. | 1974 | 4370343 | |
| the in vitro synthesis of avian myeloblastosis viral rna sequences. | isolated nuclei, prepared from myeloblasts of chicks infected with avian myeloblastosis virus, synthesize rna sequences present in avian myeloblastosis viral rna. these sequences are also formed during transcription of chromatin, isolated from myeloblasts, by dna-dependent rna polymerases purified from escherichia coli or calfthymus. in the latter case, transcription is alpha-amanitin sensitive. formation of hybrids between rna and avian myeloblastosis virus dna probes has been monitored by the ... | 1974 | 4370472 |
| structural studies of avian myeloblastosis virus: absence of leukemogenic activity of the core component. | 1974 | 4370818 | |
| in vitro association of unique species of cellular 4s rna with 35s rna of rna tumor viruses. | 1974 | 4371170 | |
| human cancer and animal viral oncology. | 1974 | 4371267 | |
| on influence of aflatoxin b1 on the physical properties of the avian myeloblastosis virus bai strain a (amv) and on the biosynthesis of viral nucleic acids. | 1974 | 4371293 | |
| effect of chemically modified 70s rna from avian myeloblastosis virus (amv) upon the activity of amv dna polymerase. | adenine residues of 70s avian myeloblastosis virus (amv) rna are modified when reacted with chloroacetaldehyde. this modification introduces characteristic fluorescent epsilon-adenosine (epsilona) probes which were used to monitor the reaction. under suitable conditions, modified 70s(epsilona) rna was maintained intact and was inactive as a template for the amv dna polymerase. furthermore, it inhibited the reaction catalyzed by amv polymerase when 70s rna was used as template-primer and had no e ... | 1974 | 4372390 |
| molecular weight of two oncornavirus genomes: derivation from particle molecular weights and rna content. | sedimentation analysis and intensity fluctuation spectroscopy have been used in conjunction with the svedberg equation to determine the particle molecular weights of rous sarcoma virus (prague strain) and avian myeloblastosis virus (bai strain). the molecular weights of these two viruses are (294 +/- 20) x 10(6) and (256 +/- 18) x 10(6), respectively. values for the molecular weight of the rna contained in each particle have been calculated as (5.58 +/- 0.5) x 10(6) and (5.88 +/- 0.5) x 10(6). s ... | 1974 | 4372402 |
| extent of double strandedness in avian myeloblastosis virus rna. | the extent of double strandedness of avian myeloblastosis virus 70s rna has been determined from fluorescence measurements of the intercalation of ethidium bromide. we have shown that 50% of the nucleotides of 70s rna in solution are in a stable helical configuration. this value does not include small helical regions that are too unstable to permit intercalation of the dye. the avian myeloblastosis virus rna as it exists within the virion has the same degree of helicity as the free 70s rna. heat ... | 1974 | 4372407 |
| mechanistic independence of avian myeloblastosis virus dna polymerase and ribonuclease h. | differential inhibition conditions were established for the dna polymerase and rnase h activities of avian myeloblastosis virus (amv) with ether-disrupted amv and a purified enzyme preparation. the rnase h activity of ether-disrupted amv with (ra)(n).(dt)(n) and (ra)(n).(dt)(11) as substrates was inhibited 80 to 100% by preincubation with naf at a final reaction concentration of 27 to 30 mm. under these conditions, the dna polymerase activity was inhibited only 0 to 20%. similar inhibitions were ... | 1974 | 4372408 |
| protein synthesizing activities of ribosome-like structure isolated from avian myeloblastosis virus in chicken cell-free protein synthesis. | 1974 | 4372573 | |
| inhibition of rauscher leukemia virus and avian myeloblastosis virus dna polymerases by tilorone (nsc-143969) and its analogs. | 1974 | 4374997 | |
| isolation of avian nephroblastoma virus from avian myeloblastosis virus by the infectious dna technique. | 1974 | 4375132 | |
| visualization of single-stranded nucleic acid of rna tumor virus with the electron microscope. | 60-70s rna of avian myeloblastosis virus was spread with the kleinschmidt method and examined in the electron microscope after being subjected to a variety of denaturing conditions. extension of the molecules from a collapsed configuration was seen only under stringent denaturing conditions, resulting in molecules of 1-2 mum in length. longer strands of these extremely fragile molecules cannot be obtained with the methods presently available. | 1974 | 4365574 |
| presence in leukemic cells of avian myeloblastosis virus-specific dna sequences absent in normal chicken cells. | (3)h-labeled 35s rna from purified avian myeloblastosis virus (amv) was exhaustively hybridized with an excess of normal chicken dna to remove all viral rna sequences which are complementary to dna from uninfected cells. the [(3)h]rna which failed to hybridize was isolated by hydroxylapatite column chromatography which separates dna-rna hybrids from single-stranded [(3)h]rna. the residual rna hybridized to leukemic chicken dna but did not rehybridize with normal chicken dna. this demonstrates co ... | 1974 | 4365719 |
| virus release by cell colonies derived from chickens and cultures infected with avian myeloblastosis virus. | 1974 | 4366504 | |
| reliability of the rna-dna filter hybridization for the detection of oncornavirus-specific dna sequences. | denatured dna from leukemic myeloblasts or uninfected chicken embryos, immobilized on nitrocellulose filters, was hybridized to a vast excess of [(3)h]70s rna from purified avian myeloblastosis virus. the viral rna was eluted from the rna-dna hybrids, purified, and then rehybridized in solution to an excess of either leukemic or normal chicken embryonic dna. this study revealed that all the slow and the fast hybridizing viral rna sequences detectable by liquid hybridization in dna excess had hyb ... | 1974 | 4367899 |
| [infection by avian myeloblastosis virus, rapidly labeled polysomal heavy rna biosynthesis and cell differentiations]. | 1974 | 4368162 | |
| inhibition of amv dna polymerase by polyriboadenylic acid containing epsilon-adenosine residues. | 1974 | 4368824 | |
| measurement of the sequence complexity of cloned moloney murine leukemia virus 60 to 70s rna: evidence for a haploid genome. | the sequence complexity of the 60-70s rna complex from moloney murine leukemia virus (m-mulv) was determined by measuring the annealing rate of radioactively labeled virus-specific dna with m-mulv 60-70s rna in conditions of vast rna excess. the m-mulv rna annealing rate, characterized by the quantity c(r)t((1/2)), was compared with the c(r)t((1/2)) values for annealing of poliovirus 35s rna (2.6 x 10(6) molecular weight) with poliovirus-specific dna and sindbis virus 42s rna (4.3 x 10(6) molecu ... | 1974 | 4368895 |
| transfection of chicken embryo cells with dna extracted from avian virus-producing neoplastic cells. | dna isolated from avian virus-producing leukemic myeloblasts induced the production of viruses, but not morphological transformation, in cultivated chicken fibroblasts. the recovered virus had the same biological characteristics as the original avian myeloblastosis virus (amv) and produced myeloblastosis and nephroblastomas when injected into chickens. neutralization experiments with chicken anti-amv-bai strain a sera showed an antigenic community between the dna-transfected virus and the origin ... | 1974 | 4369392 |
| synthesis of dna complementary to amv rna using e. coli polymerase i. | 1974 | 4362942 | |
| complementation and phenotypic stabilization of vesicular stomatitis virus temperature-sensitive and thermolabile mutants by avian myeloblastosis virus. | 1974 | 4362994 | |
| cell-free transcription of mammalian chromatin: transcription of globin messenger rna sequences from bone-marrow chromatin with mammalian rna polymerase. | a mammalian cell-free transcriptional system was developed in which mammalian rna polymerase synthesizes globin messenger rna sequences from bone-marrow chromatin. the messenger rna sequences are detected by measurement of the ability of the transcribed rna to hybridize with globin complementary dna. the globin complementary dna is synthesized by the enzyme from avian myeloblastosis virus, rna-directed dna polymerase, with purified globin messenger rna as template. the specificity of the globin ... | 1974 | 4364529 |
| the inhibition of avian myeloblastosis virus deoxyribonucleic acid polymerase by synthetic polynucleotides. | 1974 | 4365254 | |
| comparative studies on the template-initiator requirements of the chick-embryo dna polymerase i and the avian-myeloblastosis-virus dna polymerase. | 1974 | 4361413 | |
| structural studies of avian myeloblastosis virus. selective release of ribonucleoprotein polypeptides from the core component and partial purification of the dna polymerase. | 1974 | 4376423 | |
| erroneous base-pairing induced by a chemical carcinogen during dna synthesis. | 1974 | 4431466 | |
| globin messenger rna in the thalassemia syndromes. | 1974 | 4606589 | |
| nucleotide sequences of human globin messenger rna. | 1974 | 4611306 | |
| protection of avian myeloblastosis virus (amv) dna polymerase by substrates against heat inactivation. | 1974 | 10793699 | |
| acquisition of new dna sequences after infection of chicken cells with avian myeloblastosis virus. | dna-rna hybridization studies between 70s rna from avian myeloblastosis virus (amv) and an excess of dna from (i) amv-induced leukemic chicken myeloblasts or (ii) a mixture of normal and of congenitally infected k-137 chicken embryos producing avian leukosis viruses revealed the presence of fast- and slow-hybridizing virus-specific dna sequences. however, the leukemic cells contained twice the level of amv-specific dna sequences observed in normal chicken embryonic cells. the fast-reacting seque ... | 1974 | 16789139 |
| studies on reverse transcriptase of rna tumor viruses. i. localization of thermolabile dna polymerase and rnase h activities on one polypeptide. | purified reverse transcriptase from avian myeloblastosis virus or rous sarcoma virus consists of two subunits of average mol wt of 100,000 and 60,000. the lower-molecular-weight subunit, alpha, has been isolated from avian myeloblastosis virus, rous sarcoma virus and a temperature-sensitive mutant of rous sarcoma virus, la337. subunit alpha manifests both the dna polymerase and rnase h activities associated with purified reverse transcriptase of avian rna tumor viruses. the thermal inactivation ... | 1975 | 46281 |
| binding properties of avian myeloblastosis virus dna polymerases to nucleic acid affinity columns. | a new method for the analysis and purification of the rna-directed dna polymerase of rna tumor viruses has been developed. this nucleic acid affinity chromatography system utilizes an immobilized oligo (dt) moiety annealed with poly (a). the alpha and alphabeta dna polymerases of avain myeloblastosis virus bound effectively to poly (a) oligo (dt)-cellulose. alpha dna polymerase did not bind effectively to poly (a) oligo (dt)-cellulose, poly (a)-cellulose, or to cellulose. alphabeta bound to olig ... | 1975 | 46287 |