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studies of human cells infected by leukemia viruses. 19734360197
feline leukaemia virus infection--the spectrum of associated disease and its relevance to the pathogenesis and immunology of leukaemia. 19734360200
nonhuman primates in viral oncology. 19734361530
transformation of baboon cells with feline sarcoma virus. 19734361814
specificity of antibody to the rd-114 viral polymerase. 19734121714
molecular relatedness of mammalian rna tumor viruses as determined by dna hybridization.each of six mammalian c-type viruses-including two feline leukemia viruses, three murine leukemia viruses, and the human "candidate" virus rd-114-can be distinguished from each other by hybridizing dna synthesized by viral reverse transcriptase with viral rna. characterization of the dna . rna hybrids by hydroxylapatite chromatography revealed nucleotide sequence diversity among the viruses, detectable both by the amount of cross-hybridization and by the decreased thermal stability of heterologo ...19734122422
endogenous type c virus from a cat cell clone with properties distinct from previously described feline type c virus. 19734122424
isolation of rd-114-like oncornavirus from a cat cell line.a clone of cells derived from a continuous line of cat cells (ccc) spontaneously produced an rna c-type virus (ccc virus) which did not have the group-specific antigen of the standard strains of feline leukemia viruses but did have that of the rd-114 virus. single-hit infection of a virus yielding ccc cell with only the feline leukemia virus pseudotype of murine sarcoma virus [msv(felv)] resulted in the release of a pseudotype of msv coated with the ccc virus envelope. host range, transmission o ...19734123287
feline leukemia and rd-114 virus group-specific proteins: comparison of amino terminal sequence.the major internal virion polypeptide from feline and rd-114 type c viruses has been subjected to amino terminal sequence analyses with the beckman automated sequencer. these proteins, as well as their homologs in rat and mouse viruses, begin with the sequence prolylleucylarginyl (pro-leu-arg). virus rd-114 differs from conventional feline type c viruses that show about 80 percent relatedness based on calculation of the minimum number of base changes to give equivalent coding for the protein seg ...19734123999
structural proteins of oncogenic ribonucleic acid viruses. interspec ii, a new interspecies antigen. 19734124987
comparative serological studies on type c viruses of various mammals. 19734125728
utilization of mammalian 70s rna by a purified reverse transcriptase from human myelocytic leukemic cells. 19734126334
immunological relationship of dna polymerase from human acute leukaemia cells and primate and mouse leukaemia virus reverse transcriptase. 19734126783
expression of endogenous rna c-type virus group-specific antigens in mammalian cells.highly sensitive and specific radioimmunoassays are described for quantitation of the intraspecies determinants of several mammalian c-type viral group-specific (gs) antigens. an interspecies (gs-3) immunoassay has been developed which has both the broad reactivity and great sensitivity necessary for detection of c-type viruses where intraspecies gs assays are not available. by using these immunoassays, the expression of endogenous virus-specified gs antigens in mammalian cells of different spec ...19734127032
determinants of the host range of feline leukaemia viruses. 19734127732
polypeptides of mammalian oncornaviruses. i. isolation and serological analysis polypeptides from murine and feline c-type viruses. 19734127971
detection of naturally occurring antibodies to rna-dependent dna polymerase of murine leukemia virus in kidney eluates of akr mice.specific antibodies to the rna-dependent dna polymerase (reverse transcriptase) of murine type c viruses have been isolated from the renal glomeruli of both leukemic and nonleukemic akr mice where they presumably had been deposited as immune complexes. the antibodies were shown to have sedimentation coefficients of 26s to 28s and 5s to 7s on sucrose rate zonal centrifugation. inactivation with monospecific antisera to various mouse immunoglobulins identified antibodies as being in both immunoglo ...19744129792
structural proteins of mammalian oncogenic rna viruses: multiple antigenic determinants of the major internal protein and envelope glycoprotein.the antigenic determinants of two purified protein constituents of mammalian c-type rna viruses, the major structural protein of about 30,000 daltons, and the membrane glycopeptides of about 70,000 daltons were examined by competition radioimmunoassay. by the appropriate choice of antiserum and competing proteins, it was possible to distinguish type-specific, group-specific, and interspecies determinants. both of the viral constituents were found to contain each of these three classes of antigen ...19744129838
antigenic relatedness of simian c-type viruses. 19744361918
studies on the prevalence of endogenous type c virus rd 114 in cats. 19744362006
antibody response and virus survival in cats vaccinated against feline leukaemia. 19744362156
ferritin-labelled antibody study of rd-114 virus. 19744362405
homologies among the nucleotide sequences of the genomes of c-type viruses. 19744362434
retention of significant antibody binding by surface antigens of feline leukemia cells (fla) after fixation with formaldehyde and glutaraldehyde. 19744362771
structural polypeptides of primate derived type c rna tumor viruses. 19744363057
glycolysis during early infection of feline and human cells with feline leukemia virus.a 30 to 40% increase in glucose uptake and lactic acid production was observed during a 48-h period immediately following inoculation of feline fibroblasts and hep-2 cells with feline leukemia virus. there was no evidence of morphological alterations in either cell type. immunofluorescent procedures to detect feline leukemia virus group-specific antigen revealed that the feline and hep-2 cells were infected, whereas the antigen was not found in parallel cultures of uninoculated cells. increased ...19744363231
[current views on the viral origin of cancer]. 19744363460
editorial: status report: feline leukemia. 19744363507
feline malignant mammary tumors. ii. immunologic and electron microscopic investigations into a possible viral etiology. 19744363687
the inhibition of avian myeloblastosis virus deoxyribonucleic acid polymerase by synthetic polynucleotides. 19744365254
simple, quantitative assay for both xenotropic murine leukemia and ecotropic feline leukemia viruses.a cat cell line carrying the genome of a murine sarcoma virus developed discrete foci in linear response to infection with feline leukemia virus or xenotropic murine c-type virus.19744365331
letter: status report: feline leukemia. 19744365705
mink cell line mv 1 lu (ccl 64). focus formation and the generation of "nonproducer" transformed cell lines with murine and feline sarcoma viruses. 19744366800
serological studies of normal and leukemic cats in a multiple-case leukemia cluster. 19744366982
biophysical-immunological assay for ribonucleic acid type c viruses.a biophysical and immunological method for characterization of ribonucleic acid type c virus suspensions is described. the method provides a relationship to the total viral mass concentration of the particle titer, the gs antigen titer, and the ultraviolet absorbance (268 nm) of 2% sodium dodecyl sulfate digests. data for murine, rat, feline, and hamster viruses are shown to be analogous within the test limitations. from these data, an assessment of the viral purity can be made, the structural i ...19744367458
letter: feline leukemia virus. 19744368860
properties of feline leukemia virus. ii. in vitro labeling of the polypeptides.radioactive labeling of proteins from guanidine-hydrochloride disrupted feline leukemia virus was done in vitro by attaching [(3)h]methyl groups to protein amino groups with reductive alkylation. when in vitro labeled disrupted virus was analyzed by gel filtration in the presence of 6 m guanidine-hydrochloride the expected six major and one minor protein peaks were detected. when the same preparation was analyzed by immunodiffusion, the three antigens tested were found to have retained their ant ...19744369074
studies on feline lymphosarcoma in the sydney area. 19744370400
letter: transmission of feline leukemia. 19744370955
cytological and serological studies of a feline endogenous c-type virus. 19744371205
human cancer virus vaccines and the pursuit of the practical. 19744371268
erythroid hypoplasia in cats inoculated with feline leukemia virus. 19744372379
bone lesions in cats with anemia induced by feline leukemia virus. 19744372380
antigenic analysis of transformed and nontransformed cells from human neoplasms. 19744373782
editorial: the current status of feline leukemia virus research. 19744374332
feline leukemia virus and public health awareness. 19744374458
a survey of cats and humans for prevalence of feline leukemia-sarcoma virus neutralizing serum antibodies. 19744360645
rescue of cell-transforming virus from a non-virus-producing bovine cell culture transformed by feline sarcoma virus. 19744361178
mammalian sarcoma-leukemia viruses. i. infection of feline, bovine, and human cell cultures with snyder-theilen feline sarcoma virus. 19744361179
effect of feline leukemia virus on transferrred hamster fetuses. 19744361185
differences in acute and convalescent-phase antibodies of cats infected with feline picornaviruses.complement fixation inhibiting and complement-fixing antibodies were demonstrated in sera from cats during the acute and convalescent stages, respectively, of feline picornavirus infections. complement-fixing antibody activity was present in 19s and 7s globulins, whereas complement fixation inhibiting antibody activity was confined to the 7s globulins. sera with complement-fixing antibody and sera with complement fixation inhibiting antibody were also shown to have neutralizing and precipitating ...19744211925
biochemical properties of oncornavirus polypeptides. 19744376419
characterization of a newly derived human sarcoma cell line (ht-1080). 19744132053
immunology of oncornaviruses. 19744132209
endogenous baboon type c virus (m7): biochemical and immunologic characterization. 19744132240
analysis of type specific antigenic determinants of two structural polypeptides of mouse rna c-type viruses. 19744132402
poly(2'-o-methylcytidylate)-oligodeoxyguanylate as a template for the ribonucleic acid directed deoxyribonucleic acid polymerase in ribonucleic acid tumor virus particles and a specific probe for the ribonucleic acid directed enzyme in transformed murine cells. 19744133941
antigenic studies on gibbon type-c viruses. 19744133972
structural proteins of mammalian oncogenic rna viruses: murine leukemia virus neutralization by antisera prepared against purified envelope glycoprotein.goat and rabbit antisera prepared against a purified rauscher murine leukemia virus glycoprotein (gp69/71) rapidly neutralized spleen focus-forming virus in rauscher and friend virus preparations. absorption studies revealed that most of the neutralizing activity of goat anti-rauscher virus gp69/71 serum was directed against type- and group-specific determinants.19744134470
baboon virus isolate m-7 with properties similar to feline virus rd-114.a virus (m-7) isolated from baboon placental tissue demonstrates many similarities to endogenous feline virus rd-114. immunodiffusion analysis shows a group-specific antigen (gs-1) line of identity between m-7 and rd-114. anti-rd-114 dna polymerase igg inhibits m-7 polymerase by 57% compared to 97% for rd-114. m-7 virus has helper activity as demonstrated by rescue of murine sarcoma virus (msv) from sarcoma-positive leukemia-negative human amnion cells. the host range of the rescued m-7 pseudoty ...19744134740
properties of feline leukemia virus. i. chromatographic separation and analysis of the polypeptides.rickard's strain of feline leukemia virus (felv) contains two large glycoproteins and five smaller polypeptides of molecular weights 100,000 (gp >/= 100), 70,000 (gp70), 30,000 (p30), 21,000 (p21), 15,000 (p15), 11,200 (p11), and 10,000 (p10) when chromatographed on 6% agarose in the presence of 6 m guanidine hydrochloride (guhcl). p21 is a minor component which was not previously described for mammalian leukemia-sarcoma viruses and may be analogous to the seventh protein found in avian viruses. ...19744136230
simian sarcoma and feline leukemia virus antigens: isolation of species- and interspecies-specific proteins. 19744137190
radioimmunoassay for the major structural protein of hamster type c viruses.a radioimmunoassay for the major, group-specific antigen (p30) of hamster type c viruses was developed. the test detected approximately 5 ng of viral protein per ml and was highly specific for hamster viruses when used with homologous antibody. comparison of three hamster viruses, two being mouse-hamster pseudotypes, in homologous and heterologous intraspecies assays, showed no evidence of type specificity for these proteins. the pseudotype viruses showed no evidence of mouse virus p30 antigenic ...19744138682
[virus as a cause of leukemia and blood cell disease in man]. 19744140582
structural components of rna tumor viruses. 19744140684
common cell surface antigen associated with mammalian c-type rna viruses. cell membrane-bound gs antigen.the indirect membrane immunofluorescence test and the absorption analysis of rabbit anti-felv, rabbit anti-felvp 30, and rabbit anti-mulvp 30 antisera yielded the following conclusions. an antigen shared by mammalian (murine and feline) c-type rna leukemia and sarcoma viruses was detected on the surface of cells infected or transformed by c-type viruses. the antigen was characterized as membrane-bound gs antigen bearing two determinants, membrane-bound gs-1, intraspecies-specific antigenic deter ...19744131513
oncornavirus-like particles in hela cells. ii. immunological characterization of the virus. 19744131731
natural expression of feline type-c virus genomes, prevalence of detectable felv and rd-114 gs antigen, type-c particles and infectious virus in postnatal and fetal cats. 19744142463
editorial: cat leukemia: a threat to man? 19744359320
polypeptides of mammalian oncornaviruses. ii characterization of murine leukemia virus polypeptide (p 15) bearing interspecies reactivity. 197546125
zinc reverse transcriptases from mammalian rna type c viruses. 197546146
editorial: as in cats, so in man? 197546966
inhibition of oncornavirus functions by 2'-azido polynucleotides.the 2'-azido analogs of poly(u) and poly(c), poly(duz) [poly(2'-azido-2'-deoxyuridylic acid)], and poly-(dcz [poly(2'-azido-2'-deoxycytidylic acid)], were found to inhibit the rna-directed dna polymerase (reverse transcriptase) activity of murine leukemia (moloney, rauscher) and sarcoma (moloney) virus, and feline leukemia (theilen) and sarcoma (gardner) virus, while under the same conditions the unsubstituted parent compounds failed to do so. in addition, poly(duz) and poly(dcz) inhibited the r ...197547174
the oncogene hypothesis and the search for human cancer virusus. 197547649
a p60 polypeptide in the feline leukemia virus pseudotype of moloney sarcoma virus with murine leukemia virus p30 antigenic determinants.a 60,000-dalton polypeptide (p60) has been identified in the feline leukemia virus (felv) pseudotype of moloney sarcoma virus [msv(felv)]. this polypeptide is present in the purified virus complex in concentrations greater than either the murine p30 or the feline p27. purified p60 crossreacts immunologically with murine p30 group antiserum and contains several interspecies determinants, whereas the group specific determinant of felv p27 is not detected. comparison of peptide fingerprints of p60 ...197549060
isolation of mammalian type c rna virus cross-reactive antigen and antibody by immuno-affinity chromatography. 197549291
multiple species-specific and interspecific antigenic determinants of a mammalian type c rna virus internal protein. 197549292
interspecies antigenic determinants of the reverse transcriptases and p30 proteins of mammalian type c viruses.the major internal structural proteins (p30) of type c viruses isolated from several mammalian species were studied by radioimmunoprecipitation and competitive radioimmunoassays. three antigenically distinguishable sets of interspecies determinants could be demonstrated by both methods. one set of determinants shared by viruses of rodent origin (mouse and rat) can be detected readily in feline leukemia viruses but not in other type c viral groups. the p30 proteins of murine viruses also contain ...197549443
antibody in cats to mammalian rna tumor virus interspecies antigens.the studies were undertaken to determine whether the cat, a mammalian species that carries xenotropic endogenous c-type virus(es) and in addition undergoes horizontally transmitted oncogenic c-type rna tumor virus infections, responds immunologically to the mammalian c-type virus interspecies antigens. sera from normal cats and from cats with spontaneous or virus-induced neoplasms were examined for antibodies to interspecies antigen antigen by complement-fixation inhibition, by inhibition of the ...197550133
[proceedings: some biochemical properties of bovine leukemia virus]. 197550808
partial characterization of c-type particles in a cell line (wr-9) derived from a rat epidermoid carcinoma of spontaneous origin.a c-type virus continuously released from a cell line (wr-9) derived from a spontaneous epidermoid carcinoma was purified by means of large-scale tissue culture techniques and high-volume zonal centrifuges. with the use of relatively pure virus concentrates, partial characterization of the virus has been accomplished. up to 60 liters of spent culture medium from relatively low virus-yielding cultures were processed at a time through the model k ultracentrifuge in order to obtain quantities of vi ...197550883
antigenic determinants of the 70,000 molecular weight glycoprotein of woolly monkey type c rna virus.the 70,000 molecular weight glycoprotein (gp70) of a type-c rna virus originally isolated from a woolly monkey has been partially purified and immunologically characterized. evidence that this viral protein is viral coded was derived from studies showing its antigenic properties to be unaltered by virus passage in cells of different species. a broadly reactive competition immunoassay was developed utilizing antiserum prepared against feline leukemia virus to precipitate 125i-labeled woolly monke ...197551882
essential arginyl residues in reverse transcriptase. 197552361
evolution of type c viral genes: origin of feline leukemia virus.reiterated gene sequences related to the rna of feline leukemia virus (felv) are detected in all tissues of domestic cats and their close felis relatives but not in more distantly related felis species. partially homologous viral gene sequences are found in rodent, and particularly rat, dna. together with the immunologic relationships observed between felv and endogenous rodent type c viruses, the results lead to the conclusion that felv-related genes were transmitted from a rodent to cat ancest ...197552892
species and interspecies radioimmunoassays for rat type c virus p30: interviral comparisons and assay of human tumor extracts.the major internal protein, p30, of rat type c virus (ralv) was purified and utilized to establish intra- and interspecies radioimmunoassays. three rat viruses were compared in homologous and heterologous intraspecies assays with no evidence of type specificity. the only heterologous viruses to give inhibition in these species assays were the feline (felv) and hamster (halv) type c viruses; these reactions were incomplete and required high virus concentrations. an interspecies assay using a goat ...197554444
association of feline leukemia virus with lymphosarcoma and other disorders in the cat.two hundred fifty boston cats with disorders such as lymphosarcoma, myeloproliferative disease, anemia, glomerulonephritis, pregnancy abnormalities, feline infectious peritonitis, toxoplasmosis, and various bacterial infections were examined for feline leukemia virus (felv) by immunofluorescence. antibody titers against feline oncornavirus-associated cell membrane antigen (focma) were tested in 133 of these cats. the tests for felv and focma antibody were also conducted among healthy cats not kn ...1975163223
anemia associated with feline leukemia virus infection in cats.the types of anemia associated with natural and experimental feline leukemia virus (felv) infection in cats were investigated. in one experiment, 10 kittens were inoculated neonatally with jarrett felv-1, an isolate of subgroup a; 6 developed anemia a few weeks later. this anemia was characterized by macrocytosis, normoblastosis, increased erythropoiesis in the bone marrow, and extramedullary hematopoiesis in the spleen. anemia was transient and nonfatal and occurred before the onset of lympoid ...1975163317
phosphoproteins: structural components of oncornaviruses.oncornaviruses, which contain a virion-associated protein kinase, were found to possess phosphoproteins as virion structural components. one major phosphoprotein common to strains of laboratory and wild mouse oncornaviruses and a strain of feline leukemia virus was shown to be a polypeptide of about 12, 000 mol wt. in addition to this, the kirsten strain of murine sarcoma virus contained a second major phosphoprotein of about 10, 000 mol wt, and mouse erythroblastosis virus contained a second ma ...1975163371
feline leukaemia virus and its clinical effects in cats.feline leukemia virus (felv) infection is common among cats where contact is high. the virus can be transmitted readily between cats. it causes a variety of haemopoietic and lymphoid neoplasms; the most common types are alimentary, multicentric and thymic lymphosarcoma and lymphatic leukaemia. the virus is involved in the aetiology of certain other diseases including anaemia, glomerulonephritis and an immunosuppressive syndrome which predisposes cats to intercurrent infections. many infected cat ...1975163515
a mixed-immunoglobulin rosette technique for detection of antibody to feline oncornavirus-associated cell membrane antigen.a mixed-immunoglobulin rosette technique has been developed for the detection of antibodies to feline oncornavirus-associated cell membrane antigens. lymphoblastoid cells infected with feline leukemia virus were incubated with test sera and then fixed in paraformaldehyde. they were then exposed to a rabbit anti-cat immunoglobulin g serum. antigen-antibody reactions were detected by mixing the cells with sheep erythrocytes sensitized with cat anti-sheep red blood cell serum; the presence of cat a ...1975163696
naturally occurring persistent feline oncornavirus infections in the absence of disease.healthy feline leukemia virus (felv)-infected cats from leukemia cluster environments were followed for up to 23 months for development of disease and evidence of alteration in the hemogram. the incidence of disease development in felv-postive cats was more than fivefold higher than the incidence for felv-negative cats. ten cases of leukemia developed in 69 infected cats, whereas one case of leukemia occurred in 59 uninfected cats. the incidence for development of diseases other than leukemia wa ...1975163793
editorial: advances in leukaemia virus research. 1975164081
feline oncornavirus-associated cell membrane antigen. ii. antibody titers in healthy cats from household and laboratory colony environments.antibody titers to the feline oncornavirusassociated cell membrane antigen (focma) were determined for 447 healthy cats from laboratory colony and household environments. only 2.7 percent of 221 cats from colony environments were antibody positive as compared to 50.4 percent of 256 cats from household environments. incidence of focma antibody and geometric mean antibody titer for pet cats from new york city representing single cat apartment habitats were substantially lower than values for unscr ...1975164562
feline oncornavirus-associated cell membrane antigen. iii. antibody titers in cats from leukemia cluster households.serum samples from 182 healthy cats residing in environments known to have a natural exposure to feline leukemia virus (felv) were examined for the presence of antibody to the feline oncornavirus-associated cell membrane antigen. the number included 138 cats from leukemia-"cluster" households. such cats previously showed greatly increased frequencies of felv infection, as determined by the virus group-specific antigen (gasa) in peripheral blood leukocytes and platelets. the geometric mean antibo ...1975164563
aberrant viruses in cells infected with murine sarcoma virus-feline leukemia virus.this study describes an unusual type of virus seen when a feline leukemia virus (felv) pseudotype of murine sarcoma virus (musv) obtained by cocentrifugation procedures infected feline embryo cells (fef) and two crandell cat cell lines (crfk1, crfk2). when all three cell cultures were infected with musv-felv, only fef and crfk2 were transformed and only these showed normal and aberrant virus. the crfk1 infected with musv-felv did not transform but did replicate normal type-c virus with a 50-a in ...1975164569
viral-related information in oncornavirus-lik particles isolated from cultures of marrow cells from leukemic patients in relapse and remission.characterization of ribonucleic acid content of particles released from cultures of marrow cells of leukemic patients indicates the presence of rna molecules of size and base sequence characteristic of oncornarviruses. seventeen marrow samples obtained from leukemic patients in relapse or in a chronic phase of the disease yielded particles containing high-molecular-weight rna with a sedimentation velocity (about 70 s) similar to that obtained for murine oncornavirus rna. eight of nine marrow sam ...1975164662
demonstration of feline leukemia virus antibody in cats by passive hemagglutination (38583).two felv fractions from sephadex g-150 gel filtration were used to sensitize rbc for the pha test. when the cells were coated with the fraction from the second peak consisting mostly of a mojor gs antigen of felv, no antibodies could be detected either in hyperimmunized cat sera or sera from leukemic cats, whereas antibodies were readily detectable in immunized rabbits. using cells coated with the first peak eluants or tween-ether disrupted felv, pha antibodies were detected in cat sera. there e ...1975164675
affinity chromatography of viral dna polymerases on pyran-sepharose.pyran covalently linked to cyanogen bromide-activated sepharose has been shown to be an effective affinity matrix for several viral dna polymerases. differential salt elution of viral compared with cellular polymerases, as well as substrate elution, suggests the affinity nature for the matrix. unlike some other affinity systems described, pyran-sepharose is totally resistant to nuclease digestion and is stable at 4 degrees for several months. dna polymerases isolated from several viruses by dete ...1975165485
infectious spread and control of feline leukemia virus. 1975165603
differential host range of viruses of feline leukemia-sarcoma complex. 1975166488
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