Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| antibodies to vesicular stomatitis new jersey type virus in a population of white-tailed deer. | 1986 | 3012128 | |
| conservation of potential phosphorylation sites in the ns proteins of the new jersey and indiana serotypes of vesicular stomatitis virus. | a full length cdna copy of the ns mrna of the missouri strain (hazelhurst subtype, new jersey serotype) of vesicular stomatitis virus (vsv) has been cloned and sequenced. the mrna is 856 nucleotides long (excluding polyadenylic acid) and encodes a protein of 274 amino acids (mol. wt. 31 000). comparison with the ns gene of the ogden strain (concan subtype, new jersey serotype) showed 15% difference at the nucleotide level and 10% difference at the amino acid level; the majority of the changes we ... | 1986 | 3014048 |
| antibodies to vesicular stomatitis virus in populations of feral swine in the united states. | from 1979 to 1985, 941 feral swine (sus scrofa) from 53 locations in 15 states were serologically tested for antibodies to vesicular stomatitis virus (vsv). antibodies to new jersey serotype vsv were present in 75 swine from five locations in arkansas, florida, georgia, and louisiana. within these populations, antibody prevalences ranged from 10 to 100%. no antibodies to indiana serotype were detected. | 1986 | 3016348 |
| an enzyme-linked immunosorbent assay for the detection of bovine antibodies to vesicular stomatitis virus. | an indirect enzyme-linked immunosorbent assay (elisa) was developed to detect bovine antibody to vesicular stomatitis virus (vsv). serum samples from cows experimentally infected with the new jersey serotype of vsv (vsv-nj) were assayed by the elisa and serum-neutralization (sn) assay. the elisa was as sensitive as the sn assay in detecting bovine antibody to vsv. the correlation between sn titers and elisa values at absorbance at 405 nm was statistically significant. the elisa was not specific ... | 1986 | 3017162 |
| in vitro exposure of preimplantation bovine embryos to vesicular stomatitis virus. | vesicular stomatitis virus new jersey serotype (vsv-nj) adhered to 14 of 20 zonae pellucidae intact (zp-i) bovine embryos exposed in vitro. the vsv-nj-exposed zp-i bovine embryos were washed by a single- or multiple-pipette procedure. the multiple-pipette washing procedure was more efficient in removing unattached virus than the single-pipette procedure, but neither washing procedure was effective in consistently removing attached virus from zp-i embryos. the virus plaque assay with vero-maru ce ... | 1986 | 3020081 |
| defective interfering particles of vsvnj (ogden), generated by heat treatment, contain multiple internal genomic deletions. | defective interfering (di) particles have been isolated from a heat-resistant strain of the new jersey (ogden) serotype of vesicular stomatitis virus (vsv). most of these di particles contain various portions of all five cistrons of vsv. the two largest di particles, nj-121 and nj-pg2, represent approximately 60% of the standard virus genome and contain both the positive and negative strand leader rna templates. these two di particles are transcriptionally active and synthesize both the positive ... | 1986 | 3022478 |
| identification of a domain within the phosphoprotein of vesicular stomatitis virus that is essential for transcription in vitro. | a full-length cdna copy of the phosphoprotein (ns) mrna of vesicular stomatitis virus (new jersey serotype) was inserted into pgem4 vector downstream of the promoter for bacteriophage sp6 rna polymerase. transcription of the cdna in vitro resulted in the synthesis of ns mrna, which was subsequently translated into ns protein in a cell-free rabbit reticulocyte system. the biological activity of the expressed ns protein was demonstrated by in vitro synthesis of mrna by transcription-reconstitution ... | 1986 | 3024153 |
| characterization of the mutations responsible for the electrophoretic mobility differences in the ns proteins of vesicular stomatitis virus new jersey complementation group e mutants. | temperature-sensitive (ts) mutants of vesicular stomatitis virus, new jersey serotype, classified in complementation group e contain lesions in the ns gene, which manifest as marked electrophoretic mobility differences of the mutant ns proteins in sds-polyacrylamide gels. we have cloned full-length cdna copies of the mutant ns mrnas, and have determined their nucleotide sequences. tse1 and tse3 had single nucleotide changes, and tse2 had two nucleotide changes, compared to the wild-type ns gene. ... | 1986 | 3025344 |
| characterization of virulent isolates of vesicular stomatitis virus in relation to interference by defective particles. | to investigate the role that defective interfering (di) particles might conceivably play in the epizootiology of vesicular stomatitis, two virulent new jersey (nj) isolates from the 1982-1983 epizootic in the united states (us) were compared with three laboratory adapted strains of vesicular stomatitis virus (vsv): nj hazelhurst, nj ogden and indiana san juan. successive undiluted passages showed that the virulent isolates did not readily exhibit 'autointerference' because they did not readily g ... | 1986 | 2854597 |
| arbovirus isolations from mosquitoes collected during and after the 1982-1983 epizootic of western equine encephalitis in argentina. | mosquitoes were collected in santa fe and rio negro provinces, argentina, in 1982-1983 during a western equine encephalitis (wee) epizootic. totals of 153,084 mosquitoes from santa fe province and 484 from rio negro province were tested for virus in 2,351 pools. seventeen virus strains were isolated, all from santa fe collections, as follows: 4 wee, 6 venezuelan equine encephalitis, 1 st. louis encephalitis, 2 antequera, 1 maguari, 1 melao, 1 new vesiculovirus (calchaqui), and 1 gamboa. the wee ... | 1987 | 2880521 |
| a newly recognized vesiculovirus, calchaqui virus, and subtypes of melao and maguari viruses from argentina, with serologic evidence for infections of humans and horses. | in 1983, 17 virus strains were isolated from mosquitoes collected during an outbreak of western equine encephalitis in santa fe province, argentina. strains of western equine encephalitis, venezuelan equine encephalitis, st. louis encephalitis, and antequera viruses were isolated, as were several bunyaviruses of the california and bunyamwera serogroups and a new vesiculovirus. complement fixation and neutralization tests were used to identify the california serogroup virus as a subtype of melao ... | 1987 | 2880522 |
| epizootic vesicular stomatitis in colorado, 1982: epidemiologic and entomologic studies. | an epizootic of vesicular stomatitis (vs) caused by the new jersey serotype of vs virus affected livestock and humans in 14 western states in 1982-1983. epidemiological observations were made on at least 10% of the cattle in 4 dairy herds that were located in the vicinity of grand junction, colorado. high rates of neutralizing antibody to the new jersey serotype were seen in all cattle regardless of whether livestock in the dairy had clinical vs or a decrease in mild production. antibody titers ... | 1987 | 3028191 |
| epizootic vesicular stomatitis in colorado, 1982: infection in occupational risk groups. | in 1982-1983, an epizootic of vesicular stomatitis occurred in the western united states. veterinarians, research workers, and regulatory personnel who were exposed to vesicular stomatitis virus were examined for patterns of human infection and prevalence of vesicular stomatitis new jersey serotype neutralizing antibody. insight into the mechanism of transmission was sought by comparing activities of antibody-positive and antibody-negative persons. a statistically significant risk factor was a h ... | 1987 | 3028192 |
| molecular epizootiology and evolution of vesicular stomatitis virus new jersey. | vesicular stomatitis virus (vsv) has been shown previously to be capable of undergoing rapid mutational change during sequential experimental infections in various tissue culture cell systems (j. holland, k. spindler, f. horodyski, e. grabau, s. nichol, and s. vandepol, science 215:1577-1585, 1982). the present study was undertaken to determine the degree of genetic diversity and evolution of the virus under natural infection conditions and to gain insight into the epizootiology of the disease. ... | 1987 | 3029397 |
| sequences of chandipura virus n and ns genes: evidence for high mutability of the ns gene within vesiculoviruses. | the nucleotide sequence of the 3' end of the genome of chandipura (chp) virus, including the complete sequences of the nucleocapsid (n) and phosphoprotein (ns) genes was determined, principally from cloned cdnas of the n and ns mrnas. the ns mrna of chp virus is 908 bases in length and encodes a protein of 293 amino acids. comparison of the chp virus ns protein sequence with those of vesicular stomatitis virus of the new jersey serotype (vsv (nj)) and of the indiana serotype (vsv (ind] revealed ... | 1987 | 3029973 |
| antibodies to vesicular stomatitis new jersey type virus in wild and domestic sentinel swine. | wild sentinel swine on ossabaw island, chatham county, georgia, were serially bled and tested for vesicular stomatitis new jersey type virus neutralizing antibody to determine the intensity, distribution, and progression of annual viral activity. from march through september, 1984 and 1985, 112 and 226 juvenile (less than 8 months) swine, respectively, were sampled. seroconversions initially were detected on may 7, 1984 and may 18, 1985. incidence of seroconversion in wild swine reached 32% duri ... | 1987 | 3034056 |
| natural infection of humans, animals, and phlebotomine sand flies with the alagoas serotype of vesicular stomatitis virus in colombia. | five isolations of the alagoas serotype of vesicular stomatitis virus (rhabdoviridae: vesiculovirus) were made from naturally infected phlebotomine sand flies (lutzomyia spp.) collected in colombia. these are the first isolations of alagoas virus from an arthropod. replication of the virus occurred in laboratory-reared sand flies (lutzomyia longipalpis) after inoculation. bite and transovarial transmission of the virus was also demonstrated in experimentally infected sand flies. alagoas virus ne ... | 1987 | 3034087 |
| epizootic vesicular stomatitis in colorado, 1982: some observations on the possible role of wildlife populations in an enzootic maintenance cycle. | sera obtained from wild ungulates, carnivores, and rodents in colorado were tested for neutralizing (n) antibody against vesicular stomatitis, new jersey serotype (vsnj), virus to determine their involvement in the 1982 colorado vsnj epizootic in domestic animals. viremic and n antibody responses of two local rodent species to a 1982 colorado isolate of vsnj were determined in the laboratory. the rodents produced only weak viremias, but all developed n antibody. n antibody prevalences for vsnj i ... | 1987 | 3035241 |
| the fates of undermethylated mrna cap structures of vesicular stomatitis virus (new jersey) during in vitro transcription. | a dual label pulse-chase analysis employing s-adenosyl-l-[methyl-3h]methionine and [beta-32p]gtp has been devised to identify precursors to the cap structure 7mg(5')ppp(5')am present on the 5'-termini of vesicular stomatitis virus mrnas. both monomethylated cap structures, 7mg(5')ppp(5')a and g(5')ppp(5')am, have been detected in vitro in new jersey serotype reactions containing suboptimal concentrations of s-adenosyl-l-methionine. the simultaneous chasing of both radiolabeled substrates allowed ... | 1987 | 3039729 |
| homotypic and heterotypic exclusion of vesicular stomatitis virus replication by high levels of recombinant polymerase protein l. | the recombinant polymerase protein l of vesicular stomatitis virus (vsv) expressed in cos cells is able to transcribe and replicate the viral genome, resulting in complementation of temperature-sensitive polymerase mutants of vsv at the restrictive temperature (m. schubert, g. g. harmison, c. d. richardson, and e. meier, proc. natl. acad. sci. usa 82:7984-7988, 1985). here we report that the efficiency of complementation is dependent on the level of l protein expression. unexpectedly, only cells ... | 1987 | 3041035 |
| intergenic sequences of the vesicular stomatitis virus genome (new jersey serotype): evidence for two transcription initiation sites within the l gene. | the intergenic sequences of vesicular stomatitis virus of the new jersey serotype [vsv (nj): ogden strain] have been determined by dideoxy sequencing across the gene junctions of the viral rna genome using deoxyoligonucleotide primers. the n-ns, ns-m, and m-g intergenic sequences of vsv (nj) are identical to the consensus intergenic sequence for vsv of the indiana serotype [vsv (ind)]: 3'-auacu7gauugucnnnag-5' (genome sense; n denotes any nucleotide), where 3'-auacu7-5' encodes the 3' terminus a ... | 1987 | 2820143 |
| functional analysis of hypomethylation variants of the new jersey serotype of vesicular stomatitis virus. | the ts mutant f1 of vesicular stomatitis virus, new jersey serotype, directs the synthesis of undermethylated 5'-terminal cap structures in vitro. in order to determine the relationship between the ts and hypomethylation phenotypes, a spontaneous revertant rev(ts)f1 of the ts phenotype was analyzed. the revertant retained the hypomethylation phenotype. the four cap structures (gpppa, 7mgpppa, gpppam, and 7mgpppam) synthesized in mutant and revertant-directed reactions in the presence of low as w ... | 1987 | 2821679 |
| effect of melittin on transcription by vesiculovirus mutant and wild-type viruses. | the bee venom peptide melittin activated the virion transcriptase activity of three vesiculoviruses with preservation of virion structure. the kinetics of rna synthesis were similar to those observed with purified transcribing nucleoprotein (tnp) preparations. six temperature-sensitive host range (tdce) mutants of chandipura virus displayed 1.7- to 5.5-fold greater efficiencies of transcription at 39 degrees with melittin-permeabilized virions in comparison with tnp preparations. comparative stu ... | 1987 | 2822606 |
| vesicular stomatitis virus (new jersey strain) infection in two california dairy herds: an epidemiologic study. | the course of vesicular stomatitis in cattle was investigated in 2 dairy herds (a and b) located in the southern san joaquin valley of california. cattle were examined and specimens were obtained for virus isolation and for serologic survey for one year after an epizootic in december 1982. all 33 lactating cows selected for study had oral lesions, but only 19 (58%) were drooling or frothing around the mouth. lesions on feet and teats were not observed. the healing time (longer than has been repo ... | 1987 | 2824414 |
| serologic survey for evidence of exposure to vesicular stomatitis virus, pseudorabies virus, brucellosis and leptospirosis in collared peccaries from arizona. | two hundred eighteen usable serum samples were collected from hunter-killed collared peccaries (tayassu tajacu) during march 1986, in three areas of arizona. evaluations for antibodies against vesicular stomatitis virus (vsv) new jersey (nj) type, vsv indiana type, pseudorabies virus, brucellosis, and leptospirosis revealed positive test results in 8%, 0%, less than 1%, 0%, and 23% of the sera, respectively. exposure of peccaries to vsv (nj) was widespread, but variation in the prevalence of ser ... | 1987 | 2824864 |
| genome rna terminus conservation and diversity among vesiculoviruses. | sequence analysis of the rna genome termini of various vesiculovirus standard and defective interfering (di) particles demonstrated that some virus regulatory sequences and domains of virus n protein are highly conserved while others show considerable divergence. clearly, distinct rna signal sequences and protein-coding regions of these virus genomes have quite different evolutionary pressures or constraints. terminal regions of di-particle rna genomes of these viruses were found to possess self ... | 1987 | 3023698 |
| functional analysis of t lymphocyte subsets in antiviral host defense. | the role of different t cell subsets in antiviral host defense was investigated by treating thymectomized c57bl/6 and cba/j mice with monoclonal rat anti-lyt-2 or anti-l3/t4 igg 2b antibodies 14 and 10 days before infection. this treatment depleted the respective t cell subsets to undetectable levels in peripheral blood when assayed by immunofluorescence. in mice treated with anti-lyt-2, induction of cytotoxic t cells was reduced to less than 1 to 2% after intravenous infection with armstrong st ... | 1987 | 2435794 |
| antibodies against the two serotypes of vesicular stomatitis virus measured by enzyme-linked immunosorbent assay: immunodominance of serotype-specific determinants and induction of asymmetrically cross-reactive antibodies. | the serological relationship between the two vesicular stomatitis virus (vsv) strains indiana (vsv-ind) and new jersey (vsv-nj) were analyzed by using an enzyme-linked immunosorbent assay (elisa). immunoglobulin g responses, defined by their resistance to treatment with 2-mercaptoethanol, were assessed by elisa by using sucrose gradient-purified vsv or purified vsv glycoproteins (g) as antigens. when low doses (10(6) pfu) of live vsv or 10(8) pfu of uv-inactivated virus were given intraperitonea ... | 1987 | 2439706 |
| monoclonal antibodies to the glycoprotein of vesicular stomatitis virus (new jersey serotype): a method for preliminary mapping of epitopes. | of the nine antigenic determinants on the glycoprotein (g) of the new jersey serotype of vesicular stomatitis virus (vsv) identified by competitive binding of 25 monoclonal antibodies (mabs), those relegated to epitopes i, ii, iii, and iv exhibited no significant ability to neutralize virus infectivity but some nonneutralizing mabs cross-reacted by elisa with the g protein of vsv-indiana. high-titered neutralization of homotypic virus was exhibited by epitope v, vi, and vii mabs but quite variab ... | 1987 | 2446424 |
| point mutations in glycoprotein gene of vesicular stomatitis virus (new jersey serotype) selected by resistance to neutralization by epitope-specific monoclonal antibodies. | antigenic variants of the new jersey serotype of vesicular stomatitis virus (vsv-nj) were isolated and cloned by selecting virus plaques resistant to neutralization by high-titered monoclonal antibodies (mabs) directed to glycoprotein (g) epitopes v, vi, vii, or viii. the g proteins of each neutralization-resistant virus variant also exhibited markedly reduced antigenic reactivity with each corresponding epitope-specific mab as determined by enzyme-linked immuno-absorbent assay and by western bl ... | 1988 | 2451346 |
| nucleotide sequence of the l gene of vesicular stomatitis virus (new jersey): identification of conserved domains in the new jersey and indiana l proteins. | the nucleotide sequence of the l gene of vesicular stomatitis virus, new jersey serotype (hazelhurst subtype), was determined. primer extension dideoxy sequencing of genomic rna using reverse transcriptase initiated within the adjacent g gene provided a consensus sequence of 6522 nucleotides. the g/l intergenic junction spanned 21 nucleotides and contained a pseudo transcription start signal as well as two sequences (10 and 6 nucleotides in length) which are reiterated within the l coding region ... | 1988 | 2833012 |
| in vitro synthesis of large rnas by an unusual defective interfering particle of vesicular stomatitis virus. | we analyzed an unusual defective interfering (di) particle of the new jersey serotype of vesicular stomatitis virus. in vitro transcription experiments demonstrated that this di particle was unusual in that it transcribed large rnas in addition to the standard di leader rna. s1 nuclease mapping assays indicated that the large transcripts probably resulted from polymerase reinitiation downstream of the di leader termination site. a sequence related to the indiana serotype putative polymerase prom ... | 1988 | 2833624 |
| the functional domains of the phosphoprotein (ns) of vesicular stomatitis virus (indiana serotype). | a full-length cdna clone of the mrna encoding the phosphoprotein (ns) of the indiana serotype of vesicular stomatitis virus was inserted into the sp6 transcription vector. by in vitro transcription of the inserted gene followed by translation of the mrna in a rabbit reticulocyte lysate, ns protein was synthesized. the biological activity of the protein was demonstrated by rna synthesis in vitro by reconstitution with l protein and n-rna template purified from virions. using oligonucleotide-direc ... | 1988 | 2845648 |
| an enzyme-linked immunosorbent assay for the detection of vesicular stomatitis virus antigen. | an indirect, sandwich enzyme-linked immunosorbent assay (elisa) has been developed for the laboratory diagnosis of vesicular stomatitis (vs). the assay which uses rabbit and guinea-pig antisera to the purified glycoprotein antigens of serotypes new jersey (vsv-nj) and indiana (vsv-ind-1), has both high sensitivity and specificity, but has lower reactivity for the two other indiana subtypes vsv-ind-2 and vsv-ind-3. | 1988 | 2852872 |
| malpais spring virus: a new vesiculovirus from mosquitoes collected in new mexico and evidence of infected indigenous and exotic ungulates. | two virus isolates, 1 each from aedes campestris and psorophora signipennis mosquitoes collected in south central new mexico in august 1985, were shown by neutralization tests to be identical to each other, but not to any of more than 250 arthropod-borne and other viruses. electron microscopy of 1 isolate (85-488nm, chosen as the prototype) indicated that this strain shares morphologic characteristics with viruses of the family rhabdoviridae. indirect fluorescent antibody tests indicated that th ... | 1988 | 3061310 |
| genetic diversity of enzootic isolates of vesicular stomatitis virus new jersey. | the rna genomes of 43 vesicular stomatitis virus (vsv) isolates of the new jersey (nj) serotype were t1-ribonuclease fingerprinted to compare the extent of genetic diversity of virus from regions of epizootic and enzootic disease activity. forty of these viruses were obtained from central america during 1982 to 1985. the other three were older isolates, including a 1970 isolate from culex nigripalpus mosquitos in guatemala, a 1960 bovine isolate from panama, and a 1976 isolate from mosquitos (ma ... | 1988 | 2891861 |
| antigen-specific proliferative responses to vesicular stomatitis virus following immunization of cattle with inactive virus. | peripheral blood mononuclear cells (pbm) from four normal cows with no known exposure to vesicular stomatitis virus (vsv) were cultured with a new jersey (nj) serotype (ogden) vsv that had been uv-irradiated and inactivated. pbm from these animals produced no detectable proliferative response when incubated with varying concentrations of vsv-nj (ogden) ranging from 10 ng to 10 micrograms protein/ml. two of these cows were immunized with an experimental vsv-nj vaccine and their pbm were tested at ... | 1989 | 2470191 |
| epitope mapping by deletion mutants and chimeras of two vesicular stomatitis virus glycoprotein genes expressed by a vaccinia virus vector. | deletion mutants and chimeras of the glycoprotein (g) genes of vesicular stomatitis virus serotypes indiana (vsv-ind) and new jersey (vsv-nj) were cloned in plasmids and vaccinia virus vectors under control of the bacteriophage t7 polymerase promoter for expression in cv-1 cells co-infected with a t7 polymerase-expressing vaccinia virus recombinant. truncated and chimeric g proteins expressed by these vectors were tested for their capacity to react with vsv-ind and vsv-nj epitope-specific monocl ... | 1989 | 2471352 |
| interferon induction by viruses. xix. vesicular stomatitis virus--new jersey: high multiplicity passages generate interferon-inducing, defective-interfering particles. | the infectious particles of plaque-derived, low multiplicity passaged wild-type vsv of new jersey origin consistently induce about 1800 units of interferon (ifn)/10(7) aged chick embryo cells. this inducing capacity is sensitive to both uv radiation and heat (50 degrees). virus obtained after two successive high multiplicity passages in gmk-vero cells consistently induced over 25,000 units of ifn/10(7) cells. the ifn induction dose-response curve showed that one ifn-inducing particle (ifp) per c ... | 1989 | 2474895 |
| interferon induction by viruses. xviii. vesicular stomatitis virus-new jersey: a single infectious particle can both induce and suppress interferon production. | in contrast to wild-type vesicular stomatitis virus (vsv) of indiana (ind.) origin which express interferon (ifn) inducing- and ifn induction-suppressing activities as mutually exclusive properties, individual particles of wild-type vsv of the new jersey (n.j.) serotype (hazelhurst [h] isolate) paradoxically can both induce ifn and suppress its induction in cells coinfected with a potent inducer of ifn. the properties of ifn induction, and its suppression, appear to reside in the particle that m ... | 1989 | 2477474 |
| glycoprotein evolution of vesicular stomatitis virus new jersey. | a t1 ribonuclease fingerprinting study of a large number of virus isolates had previously demonstrated that considerable genetic variability existed among natural isolates of the vesicular stomatitis virus (vsv) new jersey (nj) serotype [s.t. nichol (1988) j. virol. 62, 572-579]. based on these results, 34 virus isolates were chosen as representing the extent of genetic diversity within the vsv nj serotype. we report the entire glycoprotein (g) gene nucleotide sequence and the deduced amino acid ... | 1989 | 2536983 |
| two domains distantly related to protein-tyrosine kinases in the vesicular stomatitis virus polymerase. | we have carried out an exhaustive search for amino acid sequence similarities between vesicular stomatitis virus (vsv) proteins and database entries. unexpectedly, we found that the l polymerase protein contains two blocks of sequence (residues 725-1102 and 1291-1671) with distant but statistically significant similarity to the catalytic domain of tyrosine-specific protein kinases. the first kinase-like region is most similar to members of the abl subfamily, fes and fps (26.6% and 27.3% identity ... | 1989 | 2549720 |
| isolation of vesicular stomatitis virus new jersey serotype from phlebotomine sand flies in georgia. | vesicular stomatitis virus new jersey serotype (vsnj virus) was isolated from 6 of 610 pools of phlebotomine sand flies (lutzomyia shannoni) collected on ossabaw island, ga. all isolates were from non-blooded females. infected sand flies were collected at 6 sites at 5 separate times from 3 june through 25 july 1988. thirty-five pools of culicoides ssp. and 48 pools of mosquitoes obtained in conjunction with the infected sand flies also were evaluated for vsnj virus; all were negative. concomitan ... | 1990 | 1971158 |
| [frequency of neutralizing antibodies to the vesiculovirus piry, in blood donors of uberaba, minas gerais, brazil]. | thirteen (8.0%) of 162 blood donors from uberaba-minas gerais state showed neutralizing antibodies to the vesiculovirus piry. the previous residence in rural area (p less than 0.0001) and in the cities of the são paulo state (p less than 0.05) were statistically more frequent in the piry seropositive group. | 1990 | 2135375 |
| nucleotide sequence analysis of the l gene of vesicular stomatitis virus (new jersey serotype): identification of conserved domains in l proteins of nonsegmented negative-strand rna viruses. | we have determined the nucleotide sequence of the l gene of vesicular stomatitis virus (vsv), new jersey serotype (ogden strain) by primer extension dideoxy sequencing of the genomic rna with reverse transcriptase. this analysis completes the entire genomic sequence of the vsvnj (ogden). comparison of the deduced amino acid sequence of this l protein with those reported for l proteins of indiana serotype and hazelhurst strain of new jersey serotype revealed an extensive sequence similarity among ... | 1990 | 2155516 |
| serological monitoring of vesicular stomatitis new jersey virus in enzootic regions of costa rica. | the activity of vesicular stomatitis viruses was monitored on 3 dairy farms in costa rica. antibody levels were measured and clinical disease monitored in 165 cattle during a 20 month period (1986-1988). vesicular stomatitis new jersey (vs nj) virus was shown to be enzootic on these farms by a 94.2% prevalence of neutralizing antibody; this did not vary significantly between herds. the mean prevalence of antibody to vesicular stomatitis indiana (vs in) virus was 15.2%, but was significantly high ... | 1990 | 2156464 |
| trajectory analysis of winds and vesicular stomatitis in north america, 1982-5. | outbreaks of vesicular stomatitis, serotype new jersey, during epidemics in the united states and northern mexico, 1982-5, were examined by backward trajectories of winds to investigate spread and possible sources. the outbreaks selected for analysis did not involve introduction of disease by infected animals. the findings indicate that wind could have been responsible for carrying infection from northern mexico to arizona and new mexico and thence to colorado and utah and on to wyoming, idaho a ... | 1990 | 2157606 |
| phosphoprotein and nucleocapsid protein evolution of vesicular stomatitis virus new jersey. | the entire phosphoprotein (p) and nucleocapsid (n) protein gene sequences and deduced amino acid sequences for 18 selected vesicular stomatitis virus isolates representative of the natural genetic diversity within the new jersey serotype are reported. phylogenetic analysis of the data using maximum parsimony allowed construction of evolutionary trees for the individual genes and the combined n, p, and glycoprotein (g) genes of these viruses. virtually identical rates of nucleotide substitutions ... | 1990 | 2159527 |
| vesicular stomatitis virus, new jersey serotype: replication in and transmission by lutzomyia shannoni (diptera: psychodidae). | laboratory-reared female sand flies (lutzomyia shannoni) were experimentally infected, orally and by intrathoracic inoculation, with the new jersey serotype of vesicular stomatitis (vsnj) virus. virus replication occurred in the insects following infection by both routes. virus titers greater than 10(4) plaque forming units of vsnj virus were present in heads of orally infected sand flies 12 days after virus ingestion, confirming that a persistent disseminated infection had occurred. both orally ... | 1990 | 2160198 |
| isolation of arboviruses from culicoides midges (diptera: ceratopogonidae) in colorado during an epizootic of vesicular stomatitis new jersey. | an arbovirus survey was conducted in colorado and utah during an epizootic of vesicular stomatitis new jersey (vsnj) that occurred in the western united states in 1982-1983. from 120,422 insect specimens assayed, 106 viruses were isolated. four were rhabdoviruses (vsnj), three were orbiviruses (bluetongue serotype-11), 92 were bunyamwera group (65 main drain and 27 lokern), and seven were simbu group (buttonwillow) bunyaviruses. culicoides spp. accounted for 105 viral isolates (c. (selfia) spp., ... | 1990 | 2167371 |
| vesicular stomatitis virion-associated transcriptase activity was suppressed in vitro by a synthetic 21 amino acid oligopeptide prepared to mimic the carboxy-terminus of ns protein. | to study the biological function of the ns protein of vesicular stomatitis virus (vsv), we prepared 21 species of synthetic oligopeptides with 11-21 amino acid residues, corresponding to every portion of the amino acid sequence of ns protein (indiana serotype), and tested their effects on the vs virion (vsv) transcriptase activity in vitro. only one peptide affected the virion-associated transcriptase activity of vsv indiana, by reducing the incorporation of [3h]gmp into acid-insoluble fraction ... | 1990 | 2167548 |
| polymerase errors accumulating during natural evolution of the glycoprotein gene of vesicular stomatitis virus indiana serotype isolates. | we report the entire glycoprotein (g) gene nucleotide sequences of 26 vesicular stomatitis virus indiana serotype (vsv ind) type 1 isolates from north and central america. these sequences are also compared with partial g gene sequences of vsv ind type 2 (cocal) and type 3 (alagoas) viruses and the complete g gene sequences of the more distantly related vsv new jersey (nj) and chandipura viruses. phylogenetic analysis of the g gene sequences by maximum parsimony revealed four major lineages or su ... | 1990 | 2168974 |
| spontaneous mutations leading to antigenic variations in the glycoproteins of vesicular stomatitis virus field isolates. | strains of vesicular stomatitis virus, new jersey serotype (vsv-nj), isolated from diseased cattle or swine were examined by genomic rna sequencing for genetic diversity potentially leading to antigenic variations in their type-specific glycoproteins as determined by reactivity with epitope-specific monoclonal antibodies (mabs). seven field isolates recovered in colorado, new mexico, georgia, and mexico during the widespread 1982-1985 epizootic in the western united states resembled the prototyp ... | 1990 | 1688475 |
| vesicular stomatitis new jersey virus glycoprotein gene sequence and neutralizing epitope stability in an enzootic focus. | vesicular stomatitis new jersey (vs nj) virus is capable of undergoing rapid evolution in nature and therefore has the potential for antigenic variation. we selected an area of costa rica where vs nj virus is enzootic to study whether this virus used the mechanism of antigenic variation to persist in nature. three sentinel herds and three nonsentinel herds were observed from 1986 to 1988. eleven vs nj virus isolates were collected from naturally infected cattle. remarkably, nine animals that wer ... | 1990 | 1693802 |
| cytolytic t lymphocytes from the balb/c-h-2dm2 mouse recognize the vesicular stomatitis virus glycoprotein and are restricted by class ii mhc antigens. | balb/c-h-2dm2 mice (h-2kdi-adi-eddd), a congenic strain of balb/c mice, have a deletion of the class i mhc ag, h-2ld. this gene encodes the exclusive class i mhc-restricting gene product for vesicular stomatitis virus-specific cytolytic t lymphocytes. when dm2 mice were immunized with infectious vesicular stomatitis virus, a specific ctl response was generated. these ctl lysed vsv-infected targets that expressed iad gene products, but not vsv-infected iad- targets. the ctl were used initially as ... | 1990 | 1695651 |
| effect of glycosylation on the conformational epitopes of the glycoprotein of vesicular stomatitis virus (new jersey serotype). | the conformational epitopes reactive with neutralizing monoclonal antibodies (mabs) appear to be clustered at the middle third of the glycoprotein (g) of the new jersey serotype of vesicular stomatitis virus (vsv-nj) and are flanked by two n-linked carbohydrate chains (w. keil and r.r. wagner, virology 170, 392-407, 1989). we report here studies on the effect of glycosylation on the reactivity of vsv-nj g protein derived from released virions or immunoprecipitated from pulse-labeled cells was no ... | 1991 | 1701943 |
| [a genetic study of the interaction of piry virus with drosophila]. | piry virus mutants selected for altered growth properties in drosophila melanogaster have previously been designated agd mutants. this paper presents a classification scheme for grouping some of these mutants and also presents the characteristics of some members of each group. analysis, for each agd clone, of the mean incubation time at 20 degrees c relative to the mean incubation time at 28 degrees c (t20,t28), compared to the range of similar data for wild-type virus enabled us to define 4 cla ... | 1991 | 1796217 |
| viruses transmitted by sandflies in italy. | five different phlebotomus-transmitted viruses have been isolated in italy. four of them belong to the bunyaviridae family (genus phlebovirus). sandfly fever sicilian and sandfly fever naples viruses were isolated from sick persons during world war ii, toscana and arbia viruses have been isolated more recently from phlebotomus perniciosus and p. perfiliewi. radi virus is the only phlebotomus-transmitted virus isolated in italy from p. perfiliewi, which belongs to the rhabdoviridae family (genus ... | 1991 | 1841253 |
| intracellular formation of two soluble glycoproteins in bhk cells infected with vesicular stomatitis virus serotype new jersey. | infection of bhk 21 cells with vsv serotype new jersey gave rise to three intracellular viral glycoproteins: the membrane-integrated g protein and the two soluble glycoproteins gs and gss which lacked the cytoplasmic and transmembrane domains as was deduced from limited chemical cleavage of the glycoproteins by hydroxylamine. both soluble glycoproteins were completely protected by the microsomal membrane against proteolytic digestion. the soluble glycoproteins were formed in the endoplasmic reti ... | 1991 | 1846493 |
| cloning and expression of the vesicular stomatitis virus phosphoprotein gene in escherichia coli: analysis of phosphorylation status versus transcriptional activity. | the phosphoprotein (p, previously known as ns) genes of vesicular stomatitis virus serotypes new jersey and indiana have been cloned in the escherichia coli expression vector pet-3a. transcription of p genes in these clones initiated from a phage t7 rna polymerase promoter, whereas translation was driven by the shine-dalgarno sequence and the initiator aug codon of the t7 gene 10 message. the clones were introduced into an appropriate e. coli strain in which t7 rna polymerase was expressed under ... | 1991 | 1848304 |
| myristylation of the envelope glycoprotein of vesicular stomatitis virus. | the envelope glycoprotein g of the indiana serotype of vesicular stomatitis virus was modified not only by palmitylation, but also by myristylation, both occurring at the carboxy-terminal segment. when infected chinese hamster ovary cells were grown in medium containing [3h]-myristate, the g protein and ga2, the membrane-anchoring fragment containing about 71 amino acids, were labeled. this was shown by immunoprecipitation of cell lysates or extracellular fractions of infected cultures. thin-lay ... | 1991 | 1645706 |
| antibodies to vesicular stomatitis new jersey type virus in white-tailed deer on ossabaw island, georgia, 1985 to 1989. | from 1985 to 1989, 491 serum samples were collected from white-tailed deer (odocoileus virginianus) on ossabaw island, georgia (usa) and were tested for neutralizing antibodies to new jersey and indiana type vesicular stomatitis viruses. prevalence of antibodies to vesicular stomatitis new jersey (vsnj) virus in deer for the 5-yr period was 43%. prevalence of antibodies differed by year (p less than 0.0001), and was dependent on age class (p less than 0.0001) and location on the island (p less t ... | 1991 | 1661786 |
| development and evaluation of an enzyme-linked immunosorbent assay for detection, typing, and subtyping of vesicular stomatitis virus. | an indirect sandwich enzyme-linked immunosorbent assay (elisa) has been used for vesicular stomatitis virus (vsv) typing using sets of monovalent and polyvalent rabbit/guinea pig antisera for identification of vsv types new jersey (vnj) and indiana (vind). the vind polyvalent antiserum (vind-p) detects any strain of the 3 subtypes of the vind type (vind-1, vind-2, and vind-3) with the same strong reactivity. it is also possible to subtype the vind strains using vind-p rabbit antiserum as capture ... | 1991 | 1662076 |
| phlebotomine sand flies as vectors of vesiculoviruses: a review. | phlebotomine sand flies appear to be involved in the epizootiology of 10 of the 16 currently recognized vesiculoviruses. evidence in support of this includes isolation of viruses from wild-caught male and female sand flies; demonstration of oral infection, replication, and bite transmission of viruses by sand flies; the temporal and/or spatial association between infected sand flies and infected vertebrates; and demonstration of transovarial virus transmission. the present review summarizes the ... | 1991 | 1668680 |
| lutzomyia shannoni (diptera: psychodidae): a biological vector of the new jersey serotype of vesicular stomatitis virus on ossabaw island, georgia. | the new jersey serotype of vesicular stomatitis virus (vsnj) is enzootic on ossabaw island, georgia. lutzomyia shannoni is the only phlebotomine sand fly present on the island and there is strong evidence that it is a vector of the virus at this site. this overview summarizes the studies that have been done on the island, reviews the evidence which confirms that l. shannoni is a biological vector of vsnj, and discusses remaining unknown aspects of the epizootiology of vsnj. | 1991 | 1668681 |
| identification and characterization of serine/threonine protein kinase activity intrinsic to the l protein of vesicular stomatitis virus new jersey. | a photoaffinity analogue of atp, 8-azido-adenosine 5'-triphosphate (8-n3atp), was used to probe atp-binding sites in native transcription complexes of vesicular stomatitis virus (vsv) (new jersey serotype). the analogue was found to be a substrate for a serine/threonine protein kinase that phosphorylated both the ns and l proteins of native complexes. the analogue failed to interact with the rna polymerase, another atp-utilizing activity associated with the transcription complex. kinetic analyse ... | 1992 | 1309863 |
| ultrastructural aspects of replication of the new jersey serotype of vesicular stomatitis virus in a suspected sand fly vector, lutzomyia shannoni (diptera: psychodidae). | transmission electron microscopy was used to examine replication of the new jersey serotype of vesicular stomatitis virus (vsnj) (rhabdoviridae: vesiculovirus) in lutzomyia shannoni (diptera: psychodidae), a recently implicated sand fly vector. following ingestion of an infectious blood meal, female sand flies were fixed and examined at approximately 12-hr intervals for six days. the new jersey serotype of vesicular stomatitis virus was first detected in the abdominal midgut after 34 hr of incub ... | 1992 | 1311534 |
| evaluation of an enzyme-linked immunosorbent assay for detection of antibodies to vesicular stomatitis virus in cattle in an enzootic region of mexico. | an elisa was compared with the plaque-reduction serum neutralization (prsn) test, for detection of vesicular stomatitis virus (vsv) antibodies in cattle in a vesicular stomatitis enzootic region of mexico. a total of 325 bovine serum samples were screened for vsv antibodies. the prsn test was performed, using vero cells. the elisa contained gradient-purified vsv indiana (lab strain) and vsv new jersey (hazelhurst) as the antigens. regression analysis and weighted kappa statistic were used to est ... | 1992 | 1316723 |
| biological transmission of vesicular stomatitis virus (new jersey) by simulium vittatum (diptera: simuliidae). | simulium vittatum females were shown to be competent vectors for the new jersey serotype (vsnj) of vesicular stomatitis virus (camp verde strain). seventy percent of females infected intrathoracically transmitted infectious virions in their saliva after a 10-d incubation period. when infected with virus per os, 63% of the flies tested were positive at day 10, and 45% of flies infected in this manner also secreted virus in their saliva by day 9 or 10 after infection. when ingested by s. vittatum ... | 1992 | 1322991 |
| titers of vesicular stomatitis virus, new jersey serotype, in naturally infected male and female lutzomyia shannoni (diptera: psychodidae) in georgia. | seven isolates of the new jersey serotype of vesicular stomatitis (vsnj) virus were obtained from pooled specimens of phlebotomine sand flies, lutzomyia shannoni dyar, collected on ossabaw island, chatham county, ga., in 1989 and 1990. three isolates, including two from males, were obtained from light-trapped sand flies in 1989. four isolates were obtained from pools of sand flies collected from hollow trees in 1990. three of the latter pools contained from 4.0 to 4.7 log10 of plaque-forming uni ... | 1992 | 1322992 |
| identification of heat shock protein 70 in the rabies virion. | we investigated a 73-kda polypeptide (p73), a minor component of the rabies virion (hep-flury and era strains), accounting for as much as 1% of total virion proteins. two-dimensional gel electrophoresis and immunoblotting with the antiserum against the heat shock protein 70 (hsp70) demonstrated that p73 was identical to a constitutive type of cellular hsp70. the antiserum also detected p73/hsp70 in the purified virions of other negative-stranded rna viruses, such as vesicular stomatitis virus (n ... | 1992 | 1325709 |
| phosphorylation of specific serine residues within the acidic domain of the phosphoprotein of vesicular stomatitis virus regulates transcription in vitro. | the phosphorylated state of the vesicular stomatitis virus phosphoprotein (p), an essential component of the virion-associated rna polymerase complex, has been shown to be important for the transcriptional activity of the complex. recent studies indicate that phosphorylation within the acidic domain of the p protein by cellular casein kinase ii is necessary for its activity. in an attempt to identify the exact location of the cell kinase-mediated phosphorylation, we altered specific serine and t ... | 1992 | 1326645 |
| interferon induction by viruses. xxi. vesicular stomatitis virus: interferon inducibility as a phylogenetic marker. | forty-five vesiculovirus isolates were systematically compared for their capacity to induce interferon (ifn) in chick embryo cells under conditions such that the maximum (quantum) yield of ifn per cell and the titer of ifn-inducing particles (ifp) could validly be determined. twelve isolates of the new jersey (nj) serotype of vesicular stomatitis virus (vsv) were good inducers, yielding amounts of ifn that ranged in a continuum from 300 to more than 8,000 units per 10(7) cells. these must reflec ... | 1992 | 1331259 |
| restricted replication of vesicular stomatitis virus in t lymphocytes is coincident with a deficiency in a cellular protein kinase required for viral transcription. | vesicular stomatitis virus (vsv) fails to replicate in mouse t lymphocytes unless the cells have been mitogenically stimulated with concanavalin a (con a). we have examined the possibility that the failure of vsv to replicate in unstimulated t lymphocytes can be attributed to a deficiency in a host protein kinase which activates the viral p protein by phosphorylation, thus rendering it transcriptionally competent. soluble extracts were prepared from purified mouse t lymphocytes, with or without ... | 1992 | 1335023 |
| disulfide-bonded discontinuous epitopes on the glycoprotein of vesicular stomatitis virus (new jersey serotype). | intrachain disulfide bonds between paired cysteines in the glycoprotein (g) of vesicular stomatitis virus (vsv) are required for the recognition of discontinuous epitopes by specific monoclonal antibodies (mabs) (w. keil and r. r. wagner, virology 170:392-407, 1989). cleavage by staphylococcus aureus v8 protease of the 517-amino-acid vsv-new jersey g protein, limited to the glutamic acid at residue 110, resulted in a protein (designated gv8) with greatly retarded migration by polyacrylamide gel ... | 1992 | 1374811 |
| enhancement of fish mortality by rhabdovirus infection after immunization with a viral nucleoprotein peptide. | a similar sequence to a mouse immunodominant ctl peptide (syvlqgn, single-letter amino acid code, conserved amino acids underlined) identified in the nucleoproteins of several strains of vesicular stomatitis virus (vsv) (37) was found in the nucleoproteins of viral hemorrhagic septicemia virus (vhsv) of salmonid fish (gyvyqgl in vhsv 07.71 and gyvyqgs in vhsv makah) and not in the nucleoproteins of other rhabdoviruses. the in vivo immunization of fingerling salmonid fish (rainbow trout onchorync ... | 1993 | 7513169 |
| [comparison of two neutralization tests, used in vesiculovirus piry serodiagnosis]. | a serological survey of piry was undertaken of the sera of inhabitants of catolância-bahia state, brazil. serum results obtained by a vesiculovirus neutralization test of c6/36 cells read by elisa are compared with those obtained by the classic technique, carried out on newborn mice. the agreement between the results was as high as 98.7% of the 204 sera tested and the neutralization test of c6/36 cells was chosen as the most suitable technique for the sero-survey testing. | 1993 | 8209171 |
| hiv-1 pseudotype virus containing a cocal virus genome and an hiv envelope: construction, assay and use. | a method is described for the production and assay of pseudotype viruses between human immunodeficiency virus type 1 (hiv-1) and cocal virus (cov), containing an hiv-1 envelope and a cov genome (cov(hiv)). cov(hiv) pseudotype virus is a useful tool for the investigation of a variety of questions regarding hiv entry into susceptible cells, including steps in virus binding, fusion, and internalization, and the role of molecules which inhibit entry. cov, a rhabdovirus closely related to vesicular s ... | 1993 | 8263122 |
| expression of the vesicular stomatitis virus nucleocapsid protein gene in escherichia coli: analysis of its biological activity in vitro. | the nucleocapsid (n) protein genes of vesicular stomatitis virus, serotypes new jersey and indiana, were expressed in escherichia coli bl21(de3) transformed by the recombinant expression vector pet-3a containing n genes under the control of the t7 rna polymerase promoter. following induction with isopropylthiogalactopyranoside, the n protein was synthesized, representing 10 to 20% of total cellular protein. the n protein remained in a nonaggregated form in 1 m nacl solution. the purified n prote ... | 1993 | 8382400 |
| application of indirect elisa for detection of bovine antibodies against vesicular stomatitis viruses. | two indirect enzyme-linked immunosorbent assays (i-elisas) are described for the detection of bovine serum antibody to the new jersey (nj) and indiana (in) vesicular stomatitis viruses (vsv). serum samples at a dilution of 1:200 were incubated with binary ethylenimine-inactivated vsv-nj and vsv-in type-specific antigens preadsorbed to microtiter plates. bound antibodies were detected by a murine monoclonal antibody to bovine igg1 conjugated with horseradish peroxidase. the performance of each i- ... | 1993 | 8385501 |
| molecular diversity of the lyssavirus genus. | the sequence of 5568 nucleotides of the 3' moiety of the mokola virus genome (serotype 3 of lyssaviruses) encompassing the nucleoprotein (n), phosphoprotein, matrix protein, and glycoprotein genes is presented and compared to that of the vaccinal strains of serotype 1. it allowed us to determine consensus sequences derived from the transcriptional start/stop signals and the order of protein conservation (nucleoprotein > matrix protein > phosphoprotein) in lyssaviruses. the sequences of the n gen ... | 1993 | 8386891 |
| effect of forest type on the distribution of lutzomyia shannoni (diptera: psychodidae) and vesicular stomatitis virus on ossabaw island, georgia. | we studied the effects of three forest types on multiple factors that are believed to influence the transmission of the new jersey serotype of vesicular stomatitis (vsnj) virus on ossabaw island, ga. these factors included availability of tree hole diurnal resting habitat for the presumed sand fly vector, lutzomyia shannoni dyar; relative abundance of l. shannoni; prevalence of vsnj virus infection in sand flies; and prevalence of vsnj virus antibodies in wild swine. tree hole availability, sand ... | 1993 | 8389877 |
| formalin inactivation of vesicular stomatitis virus impairs t-cell- but not t-help-independent b-cell responses. | the effects of formalin on the infectivity and immunogenicity of vesicular stomatitis virus (vsv) serotype indiana were investigated. we found that formalin inactivation of vsv prevents infection of vero cells in a concentration- and time-dependent manner, as shown by fluorometric cell analysis and inhibition of plaque formation. inactivated vsv failed to induce significant cytotoxic t-lymphocyte responses in vivo or after restimulation in vitro. in contrast, the early immunoglobulin m (igm) res ... | 1993 | 8389912 |
| immunological characterization of the vsv nucleocapsid (n) protein expressed by recombinant baculovirus in spodoptera exigua larva: use in differential diagnosis between vaccinated and infected animals. | vesicular stomatitis is a viral disease of cattle, pigs, and horses. the disease is characterized by vesicular lesions on the epithelium of the mouth, feet, and teats. the pathological lesions are virtually indistinguishable from that of foot-and-mouth disease. we have developed a recombinant baculovirus that expresses the nucleocapsid (n) protein of the new jersey serotype of vesicular stomatitis virus (vsvnj) in insect cells (sf9) and larvae (spodoptera exigua). the gene was expressed under co ... | 1993 | 8390753 |
| development of a competitive enzyme-linked immunosorbent assay for detection of bovine, ovine, porcine, and equine antibodies to vesicular stomatitis virus. | two competitive (c) enzyme-linked immunosorbent assays (elisas) were developed for the detection of antibodies to vesicular stomatitis virus (vsv) in animal sera. the assays are based upon the availability of polyclonal antibodies (pabs) from mouse ascitic fluids prepared against the new jersey (nj) and the indiana (in) vsv serotypes. the assays were performed by the immobilization of vsv-nj and vsv-in antigens on a solid phase (microtiter plate). appropriately diluted test serum mixed with an e ... | 1993 | 8394377 |
| feral swine as a potential amplifying host for vesicular stomatitis virus new jersey serotype on ossabaw island, georgia. | sentinel feral swine (sus scrofa) on ossabaw island, georgia (usa), were serologically monitored for antibodies to vesicular stomatitis new jersey serotype (vsnj) virus from 17 april to 27 august 1990. seroconversions to vsnj virus were detected in 24% of swine island-wide. differences in the incidence of seroconversion were detected between swine sampled in the pleistocene and holocene formations of the island suggesting that the presence of virus is forest type dependent. based on the consiste ... | 1993 | 8394941 |
| rapid detection of vesicular stomatitis virus new jersey serotype in clinical samples by using polymerase chain reaction. | vesicular stomatitis virus of the new jersey serotype (vsv-nj) causes vesicular disease in cattle, pigs, and horses throughout the americas. vesicular disease is clinically indistinguishable from foot-and-mouth disease (fmd). therefore, outbreaks of vesicular disease in fmd-free areas must be rapidly diagnosed by laboratory methods and affected farms must be quarantined until laboratory results confirm the absence of fmd. diagnosis is currently performed in high-containment (biosafety level 3) l ... | 1993 | 8396584 |
| piry virus antibodies in inhabitants of rio de janeiro. | 1993 | 8139469 | |
| taxonomy and evolutionary studies on lyssaviruses with special reference to africa. | currently, the lyssavirus genus is composed of four serotypes and the ungrouped european bat lyssaviruses (ebl). using previously described pcr amplification and direct sequencing methods, the n genes of 70 representative lyssaviruses collected from 40 different countries were examined. from the results, a phylogenetic tree with six branches (genotypes) was constructed: genotypes 1-4 correlated with the classification of serotypes 1-4 and in addition ebl 1 and ebl 2 were identified as two furthe ... | 1993 | 7777311 |
| cloning of the chandipura virus phosphoprotein encoding gene and its expression in escherichia coli. | chandipura (chp) virus, a member of the vesiculovirus genus within the rhabdoviridae family, was first isolated from human patients in india. the full length phosphoprotein p gene of chp have been cloned and expressed in escherichia coli using the t7 polymerase-based pet-3 series of expression vectors. under optimal conditions of induction with iptg, the recombinant p protein constituted 35% of the total bacterial protein. the bacterially expressed protein was found to be phosphate-free. deletio ... | 1994 | 7787887 |
| population dynamics of lutzomyia shannoni (diptera: psychodidae) in relation to the epizootiology of vesicular stomatitis virus on ossabaw island, georgia. | population dynamics of lutzomyia shannoni dyar were studied on ossabaw island, ga, to define further the role of this species in the epizootiology of the new jersey serotype of vesicular stomatitis (vsnj) virus. bimonthly collections of sand flies egressing from hollow trees from april to november 1991 indicated that there were three generations of sand flies. data from light trap collections from 1986 through 1989 indicated that similar seasonal cycles occurred during previous years. at this si ... | 1994 | 7815397 |
| hosts of lutzomyia shannoni (diptera: psychodidae) in relation to vesicular stomatitis virus on ossabaw island, georgia, u.s.a. | hosts of lutzomyia shannoni dyar, a suspected biological vector of the new jersey serotype of vesicular stomatitis (vsnj) virus, were determined using an indirect enzyme-linked immunosorbent assay (elisa) of 333 blood-fed female sandflies collected from their diurnal resting shelters on ossabaw island, georgia, u.s.a. sandflies had fed primarily on white-tailed deer (odocoileus virginianus) (81%) and to a lesser extent on feral swine (sus scrofa) (16%), two species of host infected annually with ... | 1994 | 7841487 |
| analysis of the nucleocapsid gene of lettuce necrotic yellows rhabdovirus. | the complete nucleotide sequence of the nucleocapsid (n) gene of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of plant rhabdoviruses, has been determined from cdna clones of both the viral genomic and messenger rnas. the previously identified n gene was adjacent to the 3' leader rna and began at position 91 from the 3' end of the lnyv genome. analysis of the sequence showed that the n mrna contained a 78-nt 5' untranslated region, followed by a 1377-nt open ... | 1994 | 8030210 |
| structural and antigenic analysis of the nucleoprotein of bovine ephemeral fever rhabdovirus. | the nucleotide sequence of the bovine ephemeral fever virus (befv) genome has been determined from the 3' terminus to the end of the nucleoprotein (n) gene. the 3' leader sequence comprises 50 nucleotides and shares a common terminal three nucleotides (3'-ugc-) and a downstream u-rich domain with vesicular stomatitis virus (vsv) and rabies virus. the n gene comprises 1328 nucleotides from the transcription initiation consensus sequence (aacagg) to the conserved transcription termination-poly(a) ... | 1994 | 8046391 |
| determinants of serotype specificity in transcription of vesicular stomatitis virus synthetic nucleocapsids. | transcription of the nucleocapsid template of vesicular stomatitis virus (vsv) is serotype specific, since the viral rna polymerase of the vsv indiana (ind) serotype transcribes the ind nucleocapsid but not the nucleocapsid of the vsv new jersey (nj) serotype and, similarly, the nj rna polymerase transcribes only the nj nucleocapsid. we have prepared synthetic nucleocapsid templates from various combinations of purified leader gene rna and n protein of these two vsv serotypes for analysis of ser ... | 1994 | 8116233 |
| genomic organization of lettuce necrotic yellows rhabdovirus. | we have mapped the genome of lettuce necrotic yellows virus (lnyv), the type member of the genus cytorhabdovirus of the family rhabdoviridae. we have cloned and sequenced all intergenic regions and the 3' leader and 5' trailer of the negative-sense, single-stranded rna genome of lnyv. the lnyv genome appears to contain six genes, the five expected genes coding for the virion proteins, and a sixth gene of unknown function, as for sonchus yellow net virus (synv), a member of the genus nucleorhabdo ... | 1994 | 8178430 |
| first isolations of arboviruses from phlebotomine sand flies in west africa. | for the first time in west africa, arboviruses were isolated from phlebotomine sand fly pools. one strain of chandipura virus (a vesiculovirus), four strains of saboya virus (a flavivirus), and one strain of a not yet identified virus were isolated. three hundred twenty-two pools were established from a population of 33,917 sand flies caught in co2 light traps in the ferlo sahelian region of senegal from november 1991 to december 1992. this is the first isolation of chandipura virus from any art ... | 1994 | 8203705 |
| localization of t helper cell epitopes in the vesicular stomatitis virus: the nucleoprotein is responsible for serotype cross-reactive t help. | the glycoprotein (g) of vesicular stomatitis virus (vsv) is known to contain the biologically relevant sites for virus-neutralizing antibodies as well as t helper (th) cell epitopes. the capacity of other vsv proteins to elicit potent th cell responses has not yet been investigated. additionally, a short-lived cross-reactivity between the two serologically distinct vsv serotypes indiana (ind) and new jersey (nj) on the t helper cell level has been reported. here we address the question of whethe ... | 1994 | 7541205 |
| common distribution of antigenic determinants and complementation activity on matrix proteins of two vesicular stomatitis virus serotypes. | to compare the antigenic and functional domains of the matrix (m) proteins of vesicular stomatitis virus (vsv) serotypes indiana (vsv-ind) and new jersey (vsv-nj), deletion mutants and chimeras were cloned in pbsm13 and expressed as in-frame lacz fusion proteins in escherichia coli. non-cross-reactive monoclonal antibodies directed to the two antigenically distinct m proteins were tested by western blot analysis to map three epitopes of vsv-ind m protein and four epitopes of vsv-nj m protein. ep ... | 1994 | 7512123 |
| the glycoprotein g of rhabdoviruses. | rhabdoviruses show an rna-containing helically-wound nucleocapsid either enclosed by or enclosing a membrane m protein, surrounded by a lipid bilayer through which dynamic protein trimers made up of non-covalently associated monomers of glycoprotein g (g) project outside. mature monomeric rhabdoviral g has more than 500 amino acids, 2-6 potential glycosylation sites, 12-16 highly conserved cysteine residues, 2-3 stretches of a-d hydrophobic heptad-repeats, a removed amino terminal hydrophobic si ... | 1995 | 7605197 |