Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| a technique to obviate the risk of inadvertent infection of cell cultures with bovine viral diarrhoea virus. | when bovine embryonic kidneys collected at the gorgie abattoir, edinburgh were examined for evidence of infection with bovine viral diarrhoea virus (bvdv), 11 out of 26 kidneys were found to be positive. a technique that detected the presence of inadvertent bvdv in cell cultures consisted of processing and digesting the kidneys to produce cell suspensions, adding dimethyl sulphoxide and dispersing the suspensions in small aliquots that were stored frozen at - 114 degrees c. one aliquot was cultu ... | 1986 | 3013952 |
| [rapid peroxidase test for demonstrating the bovine pestivirus in cell cultures]. | different variants were tested of the immunoperoxidase method for the demonstration of the mucous disease-virus diarrhoea agent in infected cells with the use of a specific conjugated antiserum. it was found that the virus could be specifically demonstrated in infected cell cultures. the test proved rapid and readily applicable. | 1986 | 3014716 |
| [experiments to culture the bovine pestivirus in heterologous cell cultures]. | attempts were made to adapt a strain of the bovine pestivirus to cell cultures of swine kidney in a series of 15 passages. the adaptation of the virus was followed up through the production of a cytopathic effect, through comparative investigations, and by means of cytologic preparations. it was found that the cytopathic effect induced and the titer of the virus grew with increasing the number of the serial passages. immunofluorescence and cytologic data showed the progressive development of the ... | 1986 | 3014717 |
| cellular inflammatory response in the lungs of calves exposed to bovine viral diarrhea virus, mycoplasma bovis, and pasteurella haemolytica. | three, 5, or 7 days after inoculation with bovine viral diarrhea (bvd) virus (n = 12) or mycoplasma bovis (n = 12), groups of calves were exposed to aerosols of pasteurella haemolytica and were euthanatized 4 hours later. histologic lesions in the lungs and the ratios of neutrophils to alveolar macrophages, collected by bronchoalveolar lavage, were compared with those of clinically healthy calves (n = 8) and calves inoculated with bvd virus only (n = 4), m bovis only (n = 4), or p haemolytica on ... | 1986 | 3014929 |
| evidence for an immunocompromising effect of bovine pestivirus on bovid herpesvirus 1 vaccination. | five calves were given live intranasal vaccine against bovid herpesvirus 1 (bhv1) two days after intranasal inoculation of bovine pestivirus (bvdv). another 5 were vaccinated in the absence of bvdv. control unvaccinated groups were also maintained. all calves were challenged with virulent bhv1. the unvaccinated calves developed signs of infectious bovine rhinotracheitis (ibr) and both vaccinated groups showed a similar degree of clinical protection from ibr. those given bvdv before vaccination s ... | 1986 | 3016976 |
| [immunofluorescence demonstration of bovine pestivirus]. | a high-titer hiperimmune calf serum was obtained via the manifold hyperimmunization with local strains of the bovine pestivirus, cultivated in homologous cell cultures. its globulin fractions were treated with fluorescein isothiocyanate to produce a specific conjugate. the specificity of the immunofluorescent serum was demonstrated through the comparative study of cell cultures infected with various viruses. the method worked out was employed to investigate tissue cross sections from diseased ca ... | 1986 | 3020775 |
| neutralization kinetics of bovine viral diarrhea virus by hyperimmune serum: one or multi-hit mechanism. | the kinetics of bovine viral diarrhea (bvd) virus neutralization (vn) by hyperimmune serum followed first order kinetics at low dilutions (1:8 and 1:16) of hyperimmune bovine serum. a lag phase in the vn curve occurred when the serum was further diluted. addition of rabbit anti-bovine igg, but not anti-igm, significantly increased the degree of vn after bvd virus was reacted with further diluted (1:256 dilution) anti-bvd bovine hyperimmune serum. incubation of virus-hyperimmune serum (1:64 dilut ... | 1986 | 3021386 |
| preliminary serological characterization of bovine viral diarrhoea virus strains using monoclonal antibodies. | five monoclonal antibodies against the bovine viral diarrhoea (bvd) viral strain nadl were isolated and characterized by an indirect immunofluorescence assay. extensive cross-reactions were detected when the antibodies were tested with 12 heterologous bvd and four hog cholera (hc) viral strains. one antibody reacted with all strains tested. two antibodies were specific for cytopathogenic bvd viruses, but failed to react with hc virus. the other antibodies reacted to varying degrees with bvd and ... | 1986 | 3022462 |
| a blocking enzyme-linked immunosorbent assay (elisa) for bovine virus diarrhoea virus serology. | 1986 | 3022502 | |
| development of an enzyme linked immunosorbent assay (elisa) for the detection of bovine serum antibody to bovine viral diarrhoea virus. | an enzyme linked immunosorbent assay (elisa) was developed to detect antibody to bovine viral diarrhoea virus (bvdv) in bovine serum. the elisa results were compared with those of the serum neutralisation test (snt) using serums from 6 experimentally infected calves bled at intervals from 0 to 154 days postinfection and 886 field samples. the optical density (od) produced by a single dilution of test serum was compared with a standard curve and the result expressed in elisa units. despite wide v ... | 1986 | 3026300 |
| [bvd (bovine virus diarrhea) and ibr (infectious bovine rhinotracheitis): serologic studies on 35 farms]. | 1099 female cattle from 35 farms in the provinces of north brabant, gelderland and overijssel were examined for the presence of antibodies against bovine virus diarrhoea (bvd) and infectious bovine rhinotracheitis (ibr) by serum neutralisation tests. one of the farms examined was seronegative for bvd and 11 farms were seronegative for ibr. on the seropositive farms about 50% of cattle carrying their first calf had not experienced infection i.e. were seronegative (bvd 51.2%, ibr 48.3%). after cal ... | 1986 | 3027917 |
| [development of elisa for demonstrating antibodies to the swine pestivirus]. | an indirect variant of the enzyme linked immunosorbent assay with the classic form of swine fever has been worked out. the test makes it possible to assess the level of antibodies with a great number of animals over a short period of time with no special equipment. the method has proved promising in taking prophylactic measures. | 1986 | 3027960 |
| safety and efficacy of a combined parapox/bvd vaccine. | the combination of a bovine viral diarrhea-mucosal disease-attenuated vaccine strain with heat-inactivated parapoxvirus was tested with respect to safety and efficacy in mice, rabbits and fattening calves. the safety on this vaccine was guaranteed in mice and rabbits under laboratory conditions and in calves under field conditions. laboratory investigations demonstrated a strong anti-bvd antibody response in rabbits compared to antibody formation after vaccination with the monovalent bvd-vaccine ... | 1986 | 3030862 |
| production of monoclonal antibodies against swine fever virus and their use in laboratory diagnosis. | thirteen hybridoma cell lines which secrete monoclonal antibodies (mcas) against swine fever virus (sfv) strain brescia were produced. the hybrid cells resulted from fusion of p3x63-ag8.653 myeloma cells with splenocytes of balb/c mice which had been immunized with purified sfv. screening of supernatant fluids was performed by an indirect enzyme-linked immunosorbent assay (elisa) and an immunoperoxidase monolayer assay (ipma). the ipma, and an immunoperoxidase test (ipt) performed on cryostat se ... | 1986 | 2428160 |
| bvd virus infection: prospects for control. | losses resulting from pestivirus infections in cattle are of considerable importance to the livestock industry yet, until relatively recently, they have been poorly understood. the escalation of research effort in this field has started to clarify the pathogenesis and epidemiology of the associated diseases but the evolution of understanding is far from complete. sufficient information exists to indicate the means by which pestivirus infection is introduced to and maintained in populations of ca ... | 1986 | 3952969 |
| pestivirus fetopathogenicity in cattle: changing sequelae with fetal maturation. | the events which followed the introduction of a heifer into a dairy herd were consistent with the animal being persistently infected with bovine diarrhoea-mucosal disease virus. obvious damage was limited to the progeny of cows which were in the first 168 days of gestation at that time. only fetuses up to 81 days of gestation at the putative time of introduction of infection became persistently infected in calfhood and, although they exhibited body tremor, two such calves necropsied at three mon ... | 1986 | 3753809 |
| the ultrastructure of cultures of chick embryo fibroblasts containing togavirus-like particles. | the ultrastructural appearance of fibroblasts cultured from specific pathogen-free chick embryos is described. these cells contained virus-like particles approximately 50 nm in diameter which accumulated in cytoplasmic vacuoles. in their size, morphology and mode of replication these particles closely resemble viruses in the genus pestivirus, family togaviridae. | 1987 | 18766654 |
| hog cholera virus: art and facts. | the aim of this review is to summarize the informations accumulated during the two last decades on hog cholera virus, a member of the pestivirus genus. different aspects concerning the virus-host cell interactions, and the structural and antigenic properties of the virion itself are successively analyzed. despite numerous works, many basic informations are still lacking, which can be explained by the difficulties inherent to the study of this virus. | 1987 | 3304110 |
| [cultivation and demonstration of the classic swine fever virus]. | experiments were carried out for the cultivation and indication of the swine pestivirus in several continuous and in primary cell lines, using lapinized and field strains of the virus. it was demonstrated that in the various cell cultures the strains used showed varying rates of growth. in pk-15 and pig embryonic kidney cell lines, the field strains and the virulent vratsa strain replicated with no preliminary adaptation, forming numerous large fluorescent plaques at the 16th to 18th hour. in th ... | 1987 | 3307133 |
| possible role of pestiviruses in microcephaly. | 1987 | 2882354 | |
| border disease. virus-induced decrease in thyroid hormone levels with associated hypomyelination. | border disease (bd) was induced in lambs by inoculation of their dams at 50 days gestation with border disease virus (bdv) isolate #31. at birth, the clinically affected lambs had diffuse spinal cord hypomyelination, confirmed by immunocytochemical staining for myelin-associated glycoprotein and myelin basic protein. in the bd lambs, large numbers of thyroid follicular epithelial cells and scattered pituitary cells contained bdv antigen by immunofluorescence staining. double labeling techniques ... | 1987 | 2441139 |
| competitive and blocking enzyme-linked immunoassay for detection of fetal bovine serum antibodies to bovine viral diarrhea virus. | a competitive blocking enzyme-linked immunoassay (celia) was developed to detect bovine viral diarrhea virus (bvdv) antibodies in undiluted fetal bovine serum (fbs). the celia was based on competition of serum bvdv antibodies with biotin-labelled anti-bvdv immunoglobulins (ig) for a limited quantity of solid-phase bvdv antigen. antigen preparation was simple, fbs could be tested undiluted, and detergent-containing washes were unnecessary. a series of dilutions of postnatal bovine bvdv antiserum ... | 1987 | 3031112 |
| detection of bovine virus diarrhea virus in cell culture using an immunoperoxidase technique. | an indirect immunoperoxidase staining technique was developed for identifying cell cultures infected with bovine virus diarrhea virus. infected cell monolayers stained intensely while uninfected monolayers remained colorless. immunoperoxidase staining was as sensitive as direct immunofluorescence in detecting endpoint dilutions of virus suspensions. using the immunoperoxidase technique, infected monolayers were detectable by macroscopic, as well as microscopic, observation. | 1987 | 3031874 |
| experimentally induced ovine border disease: extensive hypomyelination with minimal viral antigen in neonatal spinal cord. | border disease (bd) was experimentally induced in 9 lambs by inoculation of their dams with the bd-31 strain of border disease virus (bdv) at 50 days of gestation. these ewes developed serum-neutralizing antibody titers to bdv. the diagnosis of bd in their lambs was confirmed by hairy birthcoats, intrauterine growth retardation, tremors, and hypomyelination. three clinically healthy age-matched control lambs, whose dams had been given an inoculum containing only bdv-free cell culture supernatant ... | 1987 | 3032028 |
| [bovine viral diarrhea (bvd) as the cause of calf mortality among cattle breeding stock in the wesermarsch district]. | 1987 | 3032559 | |
| cell-protective monoclonal antibodies to bovine enterovirus-3 and partial or no activity against other serotypes. | preparation of monoclonal antibodies to bovine virus diarrhea virus (bvdv) yielded some hybridoma cells that secreted monoclonal antibodies against the madin-darby bovine kidney cells. the anti-cellular monoclonal antibodies reacted with other bovine cells (bovine turbinate and testicle) but not with cell lines derived from other animal species. subclones derived from one hybridoma partially blocked the infectivity of bvdv, possibly through the binding of the monoclonal antibodies with an epitop ... | 1987 | 3033333 |
| differences in virus-induced polypeptides in cells infected by cytopathic and noncytopathic biotypes of bovine virus diarrhea-mucosal disease virus. | two biotypes of bovine viral diarrhea-mucosal disease virus are present in nature: one that induces cytopathology in infected bovine cells and the other that infects cells without overt cytopathology. infections with both types of virus yield similar amounts of infectious progeny virus. field and laboratory isolates of both biotypes of bovine viral diarrhea (bvd) virus were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis of infected cell extracts. the noncytopathic bi ... | 1987 | 3033887 |
| [demonstration of bvd virus (bovine diarrhea virus) in many cell lines]. | by specific fluorescent antibodies, it was shown that cell lines from bovine origin were contaminated with bvd virus. the same virus was also detected in cell lines from different species like horse, cat, monkey, rabbit, pig and sheep. the authors express their concern about the use of these cell lines as substrate for live virus vaccines. | 1987 | 3034131 |
| variation in the intracellular polypeptide profiles from different isolates of bovine virus diarrhoea virus. | variation of the intracellular polypeptides induced in calf testis cells by 5 cloned isolates of bovine virus diarrhoea virus (bvdv) was examined. three of the isolates were cytopathic (nadl, c 2415 and pe 515 c) and two were non-cytopathic (c 1226 and pe 515 nc) in these cells. the isolates pe 515 c and pe 515 nc were both isolated from an animal with clinical signs of mucosal disease. in cells infected with nadl, 8 virus specific proteins (vp 1 to vp 8) with molecular weights ranging from 120, ... | 1987 | 3034204 |
| characterization of bovine viral diarrhoea-mucosal disease virus-specific proteins in bovine cells. | the presence of virus-specific polypeptides in bovine viral diarrhoea-mucosal disease (bvd) virus-infected bovine cells was studied by radiolabelling in the presence of a hypertonic initiation block (hib) and by analysis by sds-page. these experiments were complemented by radioimmunoprecipitations with anti-bvd hyperimmune serum of infected cells labelled under isotonic conditions. a total of 12 polypeptides (mr 165, 135, 118, 80, 75, 62, 56 to 58, 48, 37, 32, 35 and 19, all x 10(-3)) were ident ... | 1987 | 3035061 |
| glycoproteins of bovine viral diarrhoea-mucosal disease virus in infected bovine cells. | bovine cell cultures infected with bovine viral diarrhoea-mucosal disease (bvd) virus were radiolabelled with l-[35s]methionine or d-[2-3h]mannose followed by analysis of the labelled polypeptides by radioimmunoprecipitation and polyacrylamide gel electrophoresis in one and two dimensions. six glycoproteins were detected in infected cells. two abundant species had mr of 48k and 56k to 58k while the less abundant species had mr of 118k, 75k, 65k and 25k. when cells were radiolabelled with l-[35s] ... | 1987 | 3035062 |
| demonstration of bovine viral diarrhoea virus in peripheral blood mononuclear cells of persistently infected, clinically normal cattle. | peripheral blood mononuclear cells (pbl) from cattle known to be persistently viraemic with bovine viral diarrhoea virus (bvdv) following a foetal infection, were examined for the presence of viral antigens and cell-associated infectious virus. using immunocytochemical techniques, physical separations of pbl subsets and virus isolation techniques (directly and by cocultivation) it was found that infection occurred in b and t lymphocytes, monocytes, and a group of cells designated null cells for ... | 1987 | 3037018 |
| survival of bovine virus diarrhoea virus in blood from persistently infected cattle. | 1987 | 3037761 | |
| comparison by the neutralisation assay of pairs of non-cytopathogenic and cytopathogenic strains of bovine virus diarrhoea virus isolated from cases of mucosal disease. | neutralising antibody to non-cytopathogenic and cytopathogenic strains of bovine virus diarrhoea virus (bvdv) was assayed in a microtitre test in which cultures of calf testis cells were stained by the immunoperoxidase method to detect viral replication. fourteen bvdv strains were compared in cross neutralisation tests with antisera prepared in gnotobiotic calves. ten of the strains comprised five pairs of non-cytopathogenic and cytopathogenic bvdv. each pair was isolated from an animal with muc ... | 1987 | 3037765 |
| bovine viral diarrhea virus-infected mdbk monolayer as antigen in enzyme-linked immunosorbent assay (elisa) for the measurement of antibodies in bovine sera. | a specific elisa for the detection of igg antibodies against bovine viral diarrhea virus (bvdv) has been developed and was compared with the seroneutralization (sn) titers for a total of 60 bovine serum samples. a bvdv-infected madin-darby bovine kidney (mdbk) cell monolayer served as test antigen. the following results were obtained: a coefficient of correlation (r) of 0.63 (p less than 0.01) between bvdv-elisa and sn titer in bvdv-seroconverted animals and 0.71 for all sera, including that fro ... | 1987 | 3037768 |
| enzyme-linked immunosorbent assay for the detection of antibodies to bovine virus diarrhea virus in sera from border disease virus-infected sheep. | an enzyme-linked immunosorbent assay (elisa) was established for the rapid detection of specific antibodies against the causative agent of border disease in ovine sera. polyethylene-glycol concentrated, equilibrium density gradient purified bovine virus diarrhea virus was used as test antigen. the optimal amount of antigen was 0.5 microgram/well, and the optimal concentration of conjugate was at 1/4,000 dilution. a total of 20 ovine serum samples, which had been collected from animals with or wi ... | 1987 | 3038292 |
| a direct neutralizing peroxidase-linked antibody assay for detection and titration of antibodies to bovine viral diarrhoea virus. | 1987 | 3039762 | |
| the pestiviruses: where do they belong? | in this introduction to the meeting, the author specifies the place of pestiviruses among rna viruses and briefly summarizes the main characteristics from this genus. | 1987 | 3039899 |
| molecular cloning of the bovine viral diarrhea virus genomic rna. | the genomic rna from bovine viral diarrhea virus (bvd) was cloned in e. coli. the complete sequence has been obtained and the genomic organization has been deduced. some of the bvd specific cdna have been expressed in bacteria, eucaryotic cells and in vitro. we suggest that bvdv belongs to a new family different from togaviridae and flaviviridae. | 1987 | 3039900 |
| pathogenesis and epidemiology of border disease. | the pathogenesis of bd virus infection of sheep is reviewed briefly. the most serious consequences occur when susceptible pregnant sheep are infected. the virus crosses the placenta readily and can cause foetal death with resorption, mummification or stillbirths. other lambs survive, however, and are born with varying degrees of tremor and/or hairy fleeces. many such "hairy-shaker" lambs die shortly after birth but survivors gradually recover. these survivors plus other apparently normal lambs c ... | 1987 | 3039901 |
| comparison of five cell lines for the propagation of bovine viral diarrhea and infectious bovine rhinotracheitis viruses. | a comparative study was carried out to determine the susceptibility of 5 different cell types to bovine viral diarrhea (bvd) and infectious bovine rhinotracheitis (ibr) viruses. the cell systems used were swine testicle (st), mink lung (ml), bovine turbinate (bt), porcine kidney (pk15) and equine dermal (ed) cells. for bvd virus, the titers obtained on day 8 post-infection were 10(1.13), 10(3.25), 10(4.13), 10(0.00) and 10(0.00), in st, ml, bt, pk15 and ed cells, respectively. for the ibr virus, ... | 1987 | 3041184 |
| [virus carriers: analysis of states of equilibrium between the virus and its host]. | the various mechanisms of virus persistence are described. four kinds of virus persistence are presented, depending on their epidemiological significance, persistence associated with continuous multiplication and transmission (feline calicivirus, calf rotavirus); persistence associated with continuous multiplication but discontinuous transmission (equine infectious anemia retrovirus); persistence with continuous multiplication associated with immunotolerance (feline leukaemia retrovirus; mucosal ... | 1987 | 3122626 |
| [studies of the antigen structure of pestiviruses using monoclonal antibodies]. | 1987 | 2448112 | |
| the ultrastructure of cell cultures infected with border disease and bovine virus diarrhoea viruses. | the morphology of border disease virus and bovine virus diarrhoea virus in infected bovine embryonic testis cells was examined by electron microscopy. particles which appeared to be mature virions of both viruses were similar, being roughly circular and approximately 46 nm in diameter with a 20 to 25 nm core. virus replication took place totally within the cytoplasm in association with structures formed from modified endoplasmic reticulum. | 1987 | 2821174 |
| comparison of methods for concentration and purification of bovine viral diarrhea virus. | the nadl strain of bovine viral diarrhea virus (bvdv) was concentrated by hollow fiber ultrafiltration or polyethylene glycol and purified by centrifugation through sucrose or potassium tartrate gradients. the protein content of polyethylene glycol concentrates was much lower than that of ultrafiltration concentrates. conversely, recoveries of infectivity were greater using polyethylene glycol (100%) as compared to ultrafiltration (50%). sucrose or potassium tartrate density gradients appeared c ... | 1987 | 2822746 |
| the use of monoclonal antibodies in enzyme linked immunosorbent assays for detection of antibodies to bovine viral diarrhoea virus. | 1987 | 2825449 | |
| [production of high titrating antisera against bvd virus from swine and its use for direct immunofluorescence and immunoperoxidase technics]. | 1987 | 2827985 | |
| [etiologic relation between bvd virus infections in cattle and cases with clinical suspicion of bovine virus diarrhea]. | 1987 | 2827986 | |
| [consequences of the transplacental transmission of bvd virus to cattle fetuses]. | 1987 | 2827987 | |
| [transmission of pestiviruses between animal species: experimental infection of swine with the virus of bovine virus diarrhea (bvd) and of cattle with the virus of european swine fever (esp)]. | 1987 | 2827988 | |
| frequency of persistent infection of cattle with bovine viral diarrhea-mucosal disease virus in epidemic areas. | 1987 | 2828731 | |
| neutralizing antibody development following sequential inoculation of pigs with strains of bovine viral diarrhea virus and hog cholera virus. | 1987 | 2830743 | |
| high-performance liquid chromatographic separation and immunological characterization of soluble bovine viral diarrhea virus antigen. | sodium dodecyl sulphate-polyacrylamide gel electrophoresis and western blot analysis of the protein extracts from bovine viral diarrhea virus (bvdv, singer strain) infected primary calf testicle cells (soluble antigen) showed the presence of four virus specific polypeptides of 105, 90, 84 and 67 kilodaltons (kd) the 84-kd being the most abundant. anion-exchange high-performance liquid chromatography (hplc) of soluble antigen separated the virus specific polypeptides in individual peaks while the ... | 1988 | 2832429 |
| monoclonal antibodies with neutralizing activity segregate isolates of bovine viral diarrhea virus into groups. brief report. | isolates of bovine viral diarrhea (bvd) virus were differentiated by monoclonal antibodies (moabs) reactive with the 56 kd viral polypeptide. patterns of neutralizing activity of the moabs indicate that multiple epitopes are involved in virus neutralization. | 1988 | 2833200 |
| immunofluorescence of bovine virus diarrhea viral antigen in white blood cells from experimentally infected immunocompetent calves. | a study to evaluate the detection of bovine virus diarrhea viral antigen using immunofluorescence testing of white blood cells was conducted. five colostrum-deprived calves were inoculated intravenously with a cytopathic strain of the virus. lymphocyte and buffy coat smears were prepared daily for direct immunofluorescent staining for detection of antigen. lymphocytes were separated from heparinized blood using a ficoll density procedure. buffy coat smears were prepared from centrifuged blood sa ... | 1988 | 2836047 |
| detection of cytopathic and noncytopathic bovine viral diarrhea virus in cell culture with an immunoperoxidase test. | bovine viral diarrhea virus (bvdv) antigen was detected in cell culture with an indirect immunoperoxidase (ip) procedure using a specific monoclonal antibody, and an avidin-biotin-peroxidase complex. cytopathic and noncytopathic strains of the virus showed similar patterns of ip staining until 3 days post-infection. at six days post-infection, intensity of staining decreased in cell cultures infected with noncytopathic virus, but not with cytopathic virus. the ip procedure detected bvdv antigen ... | 1988 | 2836466 |
| a genetic, pathological and virological study of congenital hypotrichosis and incisor anodontia in cattle. | two friesian cows, half-sibs by a common dam, produced four bull calves with severe congenital hypotrichosis and incisor anodontia and three normal heifers by six unrelated holstein, friesian, devon and hereford bulls. the two dams, and their dam, had coats of a short, stubbly nature and the pigmented areas appeared rusty grey rather than black. pathological examination of skin samples taken from multiple standardised sites from two of the affected calves showed a reduction in the number of larg ... | 1988 | 2836985 |
| identification of hog cholera viral isolates by use of monoclonal antibodies to pestiviruses. | a collection of 90 field isolates of hog cholera virus (hcv) was used to test the specificity of four hybridoma cell lines secreting monoclonal antibodies against pestiviruses. reaction of virus isolates and monoclonal antibodies was controlled by an indirect immunofluorescence assay (ifa). two monoclonal antibodies which had been generated against hc virus strain "alfort 187" were reactive only with hcv field isolates and an hcv reference strain but not with bovine viral diarrhoea virus (bvdv) ... | 1988 | 2837867 |
| survey on the prevalence of neutralizing antibodies to bovine viral diarrhea (bvd) virus, bovine herpes virus type 1 (bhv-1) and parainfluenza virus type 3 (pi-3) in ruminants in the djibouti republic. | 1988 | 2838248 | |
| influence of interferons alpha i1 and gamma and of tumour necrosis factor on persistent infection with bovine viral diarrhoea virus in vitro. | non-cytopathic strains of bovine viral diarrhoea virus (bvdv) readily establish persistent infections in cells of bovine origin. the involvement of endogenous interferon (ifn) on the maintenance of the infection level, as well as the effect of exogenous ifn and tumour necrosis factor alpha (tnf-alpha), was studied. although exogenous ifn suppressed the spread and replication of virus, it did not cure the infection, even when continuously present over many cell passages. tnf-alpha alone had no an ... | 1988 | 2838577 |
| molecular cloning and nucleotide sequence of the pestivirus bovine viral diarrhea virus. | the rna genome of the cytopathic nadl isolate of bovine viral diarrhea virus (bvdv) has been molecularly cloned and the nucleotide sequence determined. the cloned sequence was 12,573 nucleotides in length, corresponding to a molecular weight of 4.3 x 10(6), having a base composition of 32.2% a, 25.7% g, 22.1% u, and 20.0% c. however, the sequences at the 5' and 3' termini of the rna have not been unequivocally established. a single major open reading frame extending the length of the molecule wa ... | 1988 | 2838957 |
| proteins encoded by bovine viral diarrhea virus: the genomic organization of a pestivirus. | the genome of bovine viral diarrhea virus (bvdv) contains a single large open reading frame capable of encoding 449 kda of protein. short segments from along the length of the molecularly cloned bvdv genome were engineered so as to be expressed as bacterial fusion polypeptides in escherichia coli. these bvdv analog fusion proteins were used as immunogens to generate a panel of sequence-specific antisera. these antiserum reagents were in turn employed in immunoprecipitation analyses to identify t ... | 1988 | 2838958 |
| [the occurrence of antibodies against bovine virus diarrhea-mucosal disease virus (border disease) in sheep]. | 1988 | 2840047 | |
| prevalence of antibodies to bovine virus diarrhoea-mucosal disease virus in tanzanian cattle. | a serological survey to determine the prevalence of antibodies to bovine virus diarrhoea-mucosal disease (bvd-md) virus was conducted on 419 bovine serum samples originating from 18 of 20 regions (except mwanza and shinyanga) of the tanzania mainland. the sera were a small proportion of samples collected for the appraisal of immune response to rinderpest vaccination. the survey indicated that the virus is prevalent in cattle populations and approximately 12% of sera tested contained demonstrable ... | 1988 | 2840761 |
| selection of an appropriate pestivirus strain for border disease serodiagnosis. | 1988 | 2842926 | |
| growth of rinderpest and bovine virus diarrhoea viruses in theileria parva infected lymphoblastoid cell lines. | 1988 | 2844014 | |
| production of monoclonal antibodies to bovine virus diarrhoea virus and their reactivity with selected pestivirus isolates. | 1988 | 2844042 | |
| viral and viral protein specificity of antibodies induced in cows persistently infected with noncytopathic bovine viral diarrhea virus after vaccination with cytopathic bovine viral diarrhea virus. | neutralizing and nonneutralizing antibodies to bovine viral diarrhea (bvd) virus were detected in 3 cows persistently infected with noncytopathic bvd virus after vaccination with modified-live cytopathic bvd virus. neutralizing antibodies detected in serum samples from each persistently infected cow at 3 weeks after vaccination were highly specific for certain isolates of cytopathic bvd virus and reacted only with a viral protein with a molecular weight of 53,000. neutralizing antibodies to 1 of ... | 1988 | 2844105 |
| comparisons of the pestivirus bovine viral diarrhoea virus with members of the flaviviridae. | the molecular features of bovine viral diarrhoea virus (bvdv), a member of the pestivirus genus currently classified in the togaviridae, were examined for characteristics resembling those of the flaviviridae family. like flaviviruses, bvdv possesses a single-stranded rna genome (approx. 4.3 x 10(6) mr) deficient in a 3' poly(a) tract. this rna has a single open reading frame spanning the length of the genome in the viral rna sense (positive polarity), implying an expression strategy involving th ... | 1988 | 2844971 |
| transmission of bovine virus diarrhoea virus (bvdv) by artificial insemination (ai) with semen from a persistently-infected bull. | twelve heifers that did not have antibodies to bovine virus diarrhoea virus (bvdv) were inseminated with semen from a bull that was persistently infected with the virus and contained 10(4.0)-10(6.5) tcid50 0.1 ml-1. all 12 became infected, as indicated by seroconversion within 2 weeks of insemination. four control heifers were inseminated with virus-free semen. the virus was not transmitted to these animals in spite of close contact with the heifers inseminated with the infected semen. all the h ... | 1988 | 2845636 |
| effect in vitro of bovine viral diarrhea virus on bovine embryos with the zona pellucida intact, damaged and removed. | the in vitro effect of bovine viral diarrhea virus (bvdv) on the survival of day 7 to day 7.5 bovine embryos collected from superovulated donors was studied. fifty-four experimental embryos with the zona pellucida (zp) intact, damaged or removed were exposed to 1 x 10(4) tcd50/ml of the nadl cytopathic strain of bvdc at 37 degrees c for 24 hrs and compared to 36 control embryos that were cultured for 24 hr. seven embryos with the zp-removed were similarly exposed for 48 hrs and compared to five ... | 1988 | 2845638 |
| bvd-virus infection in peripheral blood mononuclear cells from persistently viraemic calves studied by correlative immunoelectron microscopy. | 1988 | 2847450 | |
| natural infections of pigs with bovine viral diarrhoea virus associated with signs resembling swine fever. | between 1976 and 1985 while using immunofluorescence in the laboratory diagnosis of swine fever (sf), 13 incidents of bovine viral diarrhoea virus (bvdv) infection were detected in the netherlands. ultimate differentiation between swine fever virus (sfv) and bvdv was based on herd evidence supported by comparative antibody studies on sera from pigs inoculated with the isolate and from contact pigs in the herd of origin, using reference strains of sfv and bvdv. recently differentiation of sfv and ... | 1988 | 2848298 |
| bovine viral diarrhoea virus infections in piglets born to sows vaccinated against swine fever with contaminated vaccine. | on eight farms a congenital pestivirus infection in piglets was detected which could be traced to vaccination of the dams against swine fever (sf). viruses isolated from the piglets were not recognised by monoclonal antibodies (mcas) against swine fever virus (sfv) and were shown to be bovine viral diarrhoea virus (bvdv) or border disease virus. the expected 'chinese' strain of sfv could not be demonstrated in the batch of vaccine that had been used on these farms. instead, a contaminating pesti ... | 1988 | 2848299 |
| molecular cloning of complementary dna from a pneumopathic strain of bovine viral diarrhea virus and its diagnostic application. | a pneumopathic strain of bovine viral diarrhea virus was grown in cell culture and purified. genomic ribonucleic acid was extracted, polyadenylated at the 3' end, and copied into complementary dna after oligo-dt priming. complementary dna was male double stranded and cloned into the puc9 plasmid. approximately 200 complementary dna clones varying in length from 0.5 to 2.5 kilobases were obtained. hybridization assays indicated that the sequences isolated were specific for bovine viral diarrhea v ... | 1988 | 2848623 |
| bvd virus antigens in tissues of persistently viraemic, clinically normal cattle: implications for the pathogenesis of clinically fatal disease. | 1988 | 2849295 | |
| the application of monoclonal antibody panels to characterize pestivirus isolates from ruminants in great britain. | monoclonal antibodies were prepared against bovine virus diarrhoea virus and hog cholera virus. they were used to test 101 field isolates of ruminant pestivirus in a simple binding assay using an indirect immunoperoxidase label on fixed cell cultures. the monoclonals were divided into three panels: (1) pestivirus group specific, (2) hog cholera specific, (3) selectively reactive with ruminant pestiviruses. the reaction patterns with panel 3 were analyzed by a computer spreadsheet to determine th ... | 1988 | 2849388 |
| contamination of a live virus vaccine against pseudorabies (aujeszky's disease) by an ovine pestivirus pathogen for the pig. | 1988 | 2852911 | |
| typing of cytopathic and noncytopathic bovine viral diarrhea virus reference and canadian field strains using a neutralizing monoclonal antibody. | cytopathic and noncytopathic reference strains as well as canadian field isolates of bovine viral diarrhea virus were analyzed by neutralization and immunofluorescence tests using a bovine viral diarrhea virus-specific neutralizing monoclonal antibody. results on reference strains indicated three major antigenic groups: i) nadl-like, ii) new york 1-like and iii) oregon c24v-like. field isolates could be segregated into groups i and ii and none could be typed into the group iii. it appears that m ... | 1988 | 2450629 |
| bovine viral diarrhea virus proteins: heterogeneity of cytopathogenic and non-cytopathogenic strains and evidence of a 53k glycoprotein neutralization epitope. | intracellular virus-induced polypeptides from 3 cytopathogenic and 2 non-cytopathogenic bovine viral diarrhea (bvd) virus reference strains were analyzed by radioimmunoprecipitation and polyacrylamide gel electrophoresis, using a specific bovine multivalent antiserum and a neutralizing bvd-virus monoclonal antibody. electrophoretic patterns of major proteins demonstrate extensive variation between strains. most notably, a major 80,000 (80k) polypeptide was present in all cytopathogenic strains b ... | 1988 | 2454527 |
| monoclonal antibody analyses of cytopathic and noncytopathic viruses from fatal bovine viral diarrhea virus infections. | a panel of monoclonal antibodies that recognize the two major glycoproteins of bovine viral diarrhea virus (bdv) was used to evaluate the antigenic relationship between cytopathic (cp) and noncytopathic (ncp) viruses isolated from cattle dead or dying from fatal bdv infections. various unrelated bdv isolates were initially screened by indirect immunofluorescence with monoclonal antibodies directed against the 56- to 58- and 48-kilodalton glycoproteins of the virus. a wide spectrum of reactivity ... | 1988 | 2455820 |
| a rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus. | an end point dilution microtitration assay is described that can be used for the titration of both cytopathic and non-cytopathic isolates of bovine virus diarrhoea-mucosal disease virus. indirect immunofluorescence is used to detect infected mdbk cells in the wells of terasaki plates. the virus titre is derived from the number of uninfected wells, using the poisson distribution. the assay is simple, fast and economical. titres of cytopathic virus determined by the microtitration assay and standa ... | 1988 | 2466364 |
| congenital pestivirus disease (bovine virus diarrhea) of cattle. | 1988 | 3174716 | |
| neutralizing monoclonal antibodies to bovine viral diarrhoea virus bind to the 56k to 58k glycoprotein. | a panel of murine monoclonal antibodies (mabs) against the two major glycoproteins of bovine viral diarrhoea virus (bdv) was produced and assayed by serum neutralization, radioimmunoprecipitation (rip) and immunoblotting. based on their viral polypeptide specificity and on their ability to neutralize viral infectivity, the mabs in the panel were divided into three classes: class 1 mabs reacted with the 56k to 58k glycoprotein and neutralized the virus, class 2 mabs recognized the 56k to 58k glyc ... | 1988 | 2447228 |
| new concepts in the pathogenesis, diagnosis and control of diseases caused by the bovine viral diarrhea virus. | the new information on the pathogenesis and epidemiology of mucosal disease of cattle is reviewed. it is now known that clinical mucosal disease occurs only in cattle which were infected with a pestivirus in early gestation and were born with persistent viral infection and specific immunotolerance. these animals may be clinically normal at birth but may develop fatal mucosal disease, perhaps following superinfection with another pestivirus, usually between 6 and 24 months of age. they may also r ... | 1988 | 17423063 |
| recent advances in pestivirus research. | 1989 | 2471785 | |
| effects of bovine respiratory disease viruses and isoprinosine on bovine leukocyte function in vitro. | peripheral blood mononuclear cells obtained from 4- to 6-month-old-calves were inoculated in vitro with bovine herpesvirus-1, parainfluenza-3, or bovine virus diarrhea viruses. no increase in infectious virus progeny was observed; however, the viruses were detected in the cells for at least 96 h post-infection without any significant reduction in cell viability. the three viruses, either alone or in combination, suppressed phytohemagglutinin-induced proliferation of the mononuclear cells. the gr ... | 1989 | 2477940 |
| topographical and functional mapping of epitopes on hog cholera virus with monoclonal antibodies. | competitive binding studies and antigen capture assays were done with monoclonal antibodies (mabs) raised against hog cholera virus (hcv) to map the corresponding epitopes. a model was constructed in which the 13 epitopes were situated in four distinct antigenic domains: a, b, c and d. domain a was subdivided into a1, a2 and a3. the functional relevance of this model was assessed by the characterization of pestivirus strains, by neutralization studies with the mabs, and by isolation of variants ... | 1989 | 2479712 |
| antigenic diversity of bovine viral diarrhea-mucosal disease (bvd-md) viruses recently isolated from persistently infected cattle and mucosal disease, and serologic survey on bovine sera using antigenically different bvd-md viruses. | twenty nine recent isolates of bovine viral diarrhea-mucosal disease (bvd-md) virus, 17 from persistently infected cattle and 12 from mucosal disease, were compared antigenically with the reference strains by a serum neutralization test. the reference viruses were divided into 2 groups, tentatively designated as n and k, based on the antigenic relationships in the cross-neutralization test. antigenic properties of recent isolates were considerably different with the sources of virus isolation. s ... | 1989 | 2481058 |
| viral antigen distribution in the central nervous system of cattle persistently infected with bovine viral diarrhea virus. | distribution of viral antigens in the central nervous system of 25 cattle with a persistent bovine viral diarrhea virus (bvdv) infection was studied. using a polyclonal antiserum produced in pigs and the direct immunofluorescence and immunoperoxidase technique, bvdv antigen was located exclusively in neurons. predilection sites for viral persistence were cerebral cortex and hippocampus; in other areas of brain and spinal cord, viral antigens were in single neurons or small groups of neurons. the ... | 1989 | 2536511 |
| evaluation of the quality and virological status of semen from bulls acutely infected with bvdv. | 1989 | 2537554 | |
| serological characterization of viruses isolated from experimental mucosal disease. | four cattle persistently infected with non-cytopathogenic bovine viral diarrhoea-mucosal disease (bvd-md) virus and 3 normal controls were challenge exposed to cytopathogenic bvd-md viruses that are antigenically different from the persistent viruses. two of the persistently infected cattle developed mucosal disease and became moribund on postinoculation days (pid) 28 and 14, respectively; one developed severe and chronic diarrhoea and became moribund on pid 32; and the other remained healthy un ... | 1989 | 2538022 |
| serological comparison of cytopathogenic and non-cytopathogenic bovine viral diarrhea-mucosal disease viruses isolated from cattle with mucosal disease. | ten cattle that died with mucosal disease were examined for bovine viral diarrhea-mucosal disease (bvd-md) viruses. both cytopathogenic (cp) and non-cytopathogenic (ncp) bvd-md viruses were isolated concomitantly from 9 of them, and only a cp virus was recovered from the other. then each pair of cp and ncp viruses was compared serologically by a serum neutralization test. each pair of cp and ncp viruses from the same cattle was found to be serologically indistinguishable, although a minor antige ... | 1989 | 2538669 |
| differentiation of hog cholera and bovine virus diarrhoea viruses in pigs using monoclonal antibodies. | monoclonal antibodies against hog cholera and bovine viral diarrhoea viruses were assayed on organ tissue sections of experimentally infected animals. the animals had been infected simultaneously with both viruses. the antibodies were tested using an indirect immunofluorescence test and an indirect enzyme immunoassay with a biotin/streptavidin/peroxidase detection system. a polyclonal hyperimmune serum was used as a control in direct immunofluorescence tests. both techniques based on monoclonal ... | 1989 | 2538980 |
| aetiology of kawasaki disease. | thirteen serum samples from nine children with kawasaki disease and 23 control samples gave negative results on screening for antibodies to hog cholera virus, border disease of sheep, bovine diarrhoea virus, and equine arteritis virus. the sera from two children with kawasaki disease were cytotoxic; a possible link with cytotoxin from propionibacterium acnes is considered. | 1989 | 2539788 |
| application of peroxidase labelled antibody assays for detection of porcine igg antibodies to hog cholera and bovine viral diarrhea viruses. | rapid, sensitive peroxidase labelled antibody (pla) assays using microtiter systems, were developed for detection of hog cholera virus (hcv) and cross-reacting bovine viral diarrhea virus (bvdv) antibodies in pig sera. hcv-infected pig kidney cell line (pk 15) prepared in microtiter plates were fixed and used in pla assays. after inoculation with test serum, bound antibodies (hcv/bvdv) were reacted with either horseradish peroxidase (hrp) conjugated anti-porcine immunoglobulin (h & l) or biotiny ... | 1989 | 2541152 |
| [epidemic disease in a sheep herd with a possible relationship to a pestivirus]. | in connection with a case of epidemic disease with high morbidity and mortality in a flock of sheep, a pestivirus infection could be diagnosed. pathological-anatomical abnormalities were very similar to the character of bvd/md. scientific findings on pestivirus infections in sheep are discussed. | 1989 | 2541517 |
| protection against respiratory infection with bovine virus diarrhoea virus by passively acquired antibody. | susceptibility to infection with bovine virus diarrhoea virus (bvdv) was compared for calves with varying amounts of specific antibody in their sera passively acquired from the ingestion of colostrum. challenge consisted of intranasal exposure to a strain of bvdv isolated from an outbreak of respiratory disease. resistance to infection, as judged by nasopharyngeal shedding of virus, was directly related to the titre of neutralizing antibodies in sera. besides protecting against infection of the ... | 1989 | 2541531 |
| n-terminal domains of putative helicases of flavi- and pestiviruses may be serine proteases. | recently we tentatively identified, by sequence comparison, central domains of the ns3 proteins of flaviviruses and the respective portion of the pestivirus polyprotein as rna helicases (a.e.g. et al., submitted). alignment of the n-proximal domains of the same proteins revealed conservation of short sequence stretches resembling those around the catalytic ser, his and asp residues of chymotrypsin-like proteases. a statistically significant similarity has been detected between the sequences of t ... | 1989 | 2543956 |
| experimental infection of cattle in early pregnancy with a cytopathic strain of bovine virus diarrhoea virus. | nine pregnant heifers, in early gestation (63 to 107 days), were infected intranasally or in utero with cytopathic bovine virus diarrhoea virus (bvdv) and each dam seroconverted. all nine calves developed to full term; four were stillborn, of which one had seroconverted but virus was not recovered from their tissues. one of the five liveborn calves appeared to have seroconverted in utero to an adventitious bvdv infection in late pregnancy but the remaining four were not viraemic and showed a nor ... | 1989 | 2544969 |