Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| alterations in thrombopoiesis in patients with thrombocytopenia produced by dengue hemorrhagic fever. | the causes of thrombocytopenia, one of the most dangerous symptoms in the course of dengue hemorrhagic fever (dhf), are not yet clear. we studied patients with dhf (dengue virus type 2), grouped according to days of illness, platelet counts, serum thrombopoietin (tpo) levels, and bone marrow differential counts. during the thrombocytopenic phase of disease (3rd-8th days) the tpo levels were not increased in spite of low platelet counts. beginning from the 6th-7th day in some patients, a rapid in ... | 1986 | 3808936 |
| nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue type 2 virus, jamaica genotype. | the nucleotide sequence of the 5'-terminal 2469 bases of dengue 2 (jamaica genotype) virus has been determined and the encoded proteins compared with those of yellow fever and west nile viruses, which belong to different flavivirus serogroups. the cdna clone which was sequenced contains a 5'-noncoding region of 96 nucleotides followed by a single open reading frame coding for the structural proteins 5'-c-prm(m)-e-3' and the beginning of the ns1 nonstructural protein. the amino acid sequence homo ... | 1986 | 3024394 |
| geographic classification of dengue-2 virus strains by antigen signature analysis. | dengue-2 virus strains from different locations were compared by t1-rnase-resistant oligonucleotide fingerprinting and antigen signature analysis. the latter technique involved construction of radioimmunoassays using monoclonal antibodies that recognize nine distinct dengue-2 type-specific and flavivirus cross-reactive epitopes over a range of antigen concentrations. a statistical method was used to align unknown dengue antigen concentrations in different strain preparations, allowing comparison ... | 1986 | 2429440 |
| topological mapping of unique epitopes on the dengue-2 virus ns1 protein using monoclonal antibodies. | monoclonal antibodies were produced against two distinct thai dengue-2 (den-2) virus strains isolated in 1980 from dengue haemorrhagic fever patients. nine of 36 hybridomas produced monoclonal igg antibodies which reacted in radioimmune precipitation assays with the ns1 non-structural protein (42,000 mol. wt.) from den-2-infected c6/36 (aedes albopictus) cells. the virus specificity of ns1-reactive monoclonal antibodies was determined by indirect immunofluorescence assays using llc-mk2 cells inf ... | 1987 | 2434611 |
| detection of flavivirus rna in infected cells using photobiotin-labelled hybridization probes. | ten plasmids containing viral cdna inserts of portions of the dengue virus type 2 (den-2) or kunjin virus (kun) genomes were biotinylated using photobiotin acetate and used as probes for the detection of flavivirus rna in infected vero cells. the viral cdna inserts ranged in length from 0.19 to 2.7 kilobase pairs, and represented segments of the flavivirus genome coding for structural and nonstructural proteins. in spot hybridization assays (hybridization at 60 degrees c) with rna extracted from ... | 1987 | 3031110 |
| effect of temperature on the vector efficiency of aedes aegypti for dengue 2 virus. | the effect of temperature on the ability of aedes aegypti to transmit dengue (den) 2 virus to rhesus monkeys was assessed as a possible explanation for the seasonal variation in the incidence of dengue hemorrhagic fever in bangkok, thailand. in two laboratory experiments, a bangkok strain of ae. aegypti was allowed to feed upon viremic monkeys infected with den-2 virus. blood-engorged mosquitoes were separated into two groups and retained at constant temperatures. virus infection and transmissio ... | 1987 | 3812879 |
| an electron and immunoelectron microscopic study of dengue-2 virus infection of cultured mosquito cells: maturation events. | the maturation process of dengue-2 virus in c6/36 mosquito cells was studied by electron microscopy at 12, 16, 24, 48, and 78 hours postinoculation (p.i.) and by immunoelectron microscopy at 48 and 78 hours p.i. maturing virions appeared within cytoplasmic vacuoles and on the surface of infected cells from 24 hours p.i. onward in close topographical relationship to the dense particles that occurred concurrently in the cytoplasm. the dense particles measured 25 to 35 nm in diameter; the mature vi ... | 1987 | 3813888 |
| protection of mice against dengue 2 virus encephalitis by immunization with the dengue 2 virus non-structural glycoprotein ns1. | immunization of mice with the dengue 2 virus (den 2)-specified non-structural protein ns1 provided significant protection against intracerebral challenge with the virus in the absence of detectable neutralizing or other anti-virion antibody. ns1, purified from lysates of infected vero cells by immunoaffinity chromatography, expressed an antigenic site(s) common to each of the four den serotypes, and hyperimmunization of rabbits with ns1 stimulated production of complement-fixing (cf) antibody wi ... | 1987 | 3819700 |
| monoclonal antibodies against dengue 2 virus e-glycoprotein protect mice against lethal dengue infection. | a panel of 11 murine monoclonal antibodies directed against dengue type 2 was evaluated for antigen specificity by dot immunobinding assay and western blot analysis and for in vitro and in vivo biological activities. nine of the 11 monoclonal antibodies reacted with viral e-glycoprotein based on the western blot analysis; one reacted with a 36 kd protein present in dengue-infected c6/36 mosquito cells. the nine e-glycoprotein-reactive monoclonal antibodies also neutralized dengue 2 virus in a pl ... | 1987 | 3826503 |
| detection of dengue virus rna using nucleic acid hybridization. | conditions for using slot-blot nucleic acid hybridization to quantitatively detect dengue-2 virus using a radiolabelled cdna probe, pvv17, were determined. as little as 11 plaque-forming units of virus were detected using a hybridization mixture without formamide and performing the test at 70 degrees c. while predominantly serotype-specific using stringent (65 degrees c) washing conditions, the probe detected all four dengue virus serotypes using astringent (28 degrees c) washing conditions. no ... | 1987 | 2881940 |
| antibody-dependent enhancement of dengue 2 virus in people of white descent in cuba. | 1987 | 2883360 | |
| maturation process of japanese encephalitis virus in cultured mosquito cells in vitro and mouse brain cells in vivo. | the maturation process of japanese encephalitis (je) virus in c6/36 cells in vitro and in mouse brain cells in vivo was studied by electron microscopy. in the c6/36 cell infection, 500 to 2250 virions per cell were released into the medium during the period of study; yet, no virus budding process was observed at the host cell membranes. je virions at various maturation stages appeared within the cisternae of rough endoplasmic reticulum (rer) of infected cells at 24 hours p.i.; and, although c6/3 ... | 1987 | 2889443 |
| the influence of higher temperature on dengue-2 virus infected c6/36 mosquito cell line. | dengue-2 virus infection of c6/36 cells was studied at 28 and 37 degrees c. in infected cells maintained at 28 degrees c, syncytial development was seen on day 4 postinfection, whereas at 37 degrees c, extensive syncytial development was seen by 32 h. extracellular virus titre was found to correlate with the cytopathic changes. nine dengue-2 virus specified proteins were observed in polyacrylamide analyses of cytoplasmic extracts of c6/36 infected cells. all the proteins were observed, although ... | 1987 | 2891426 |
| characterization of protease cleavage sites involved in the formation of the envelope glycoprotein and three non-structural proteins of dengue virus type 2, new guinea c strain. | amino terminal sequences of the envelope protein e and the three largest nonstructural proteins ns1, ns3, and ns5 of the new guinea c strain of dengue virus type 2 (den-2) were obtained by nucleotide and protein sequencing. clones were prepared containing cdna of den-2 virus in the plasmid puc8. the nucleotide sequences of viral cdna inserts were determined and the cdna of each clone positioned on the flavivirus genomic map by comparison of the deduced amino acid sequence with that of yellow fev ... | 1987 | 2952760 |
| immunization with a live attenuated dengue-2-virus candidate vaccine (16681-pdk 53): clinical, immunological and biological responses in adult volunteers. | a live dengue-2 (den-2) candidate vaccine (strain 16681-pdk 53), attenuated by passage in primary dog kidney cells, was tested in ten adult volunteers for evaluation of the safety, infectivity and immunogenicity of a dose of 1.9-2.7 x 10(4) plaque-forming units. five of the volunteers were nonimmune to either dengue or japanese encephalitis (je) viruses; the other five were nonimmune to dengue but immune to je. after receiving 1.0 ml of the vaccine subcutaneously, all ten volunteers developed ne ... | 1987 | 3496985 |
| profiles of antibody-dependent enhancement of dengue virus type 2 infection. | antibody-dependent infection enhancement (ade) was studied with p-388d1 mouse macrophage-like cells, 21 dengue virus type 2 (den-2) strains, and 8 monoclonal antibodies reactive with flavivirus group-specific or dengue serotype-specific determinants. testing a constant number of virions against serial dilutions of antibody for their ability to infect p-388d1 cells, a reproducible 'enhancement profile' was observed. the profile was characterized by (1) appearance, peak, decline, and disappearance ... | 1987 | 3504546 |
| increased dissemination of dengue 2 virus in aedes aegypti associated with concurrent ingestion of microfilariae of brugia malayi. | we investigated whether concurrent ingestion of dengue 2 virus and microfilariae of brugia malayi would increase viral infection and dissemination rates in aedes aegypti. infection rates were similar in mosquitoes that ingested virus alone or both virus and microfilariae concurrently. however, viral dissemination rates, as determined by recovery of dengue virus from both legs and bodies separately, were significantly greater in mosquitoes that ingested both agents concurrently than in those that ... | 1987 | 3605503 |
| susceptibility of aedes albopictus c6/36 cells to viral infection. | the susceptibility of the c6/36 clone of aedes albopictus monolayer cell cultures was determined with 46 prototype viruses passed through three subcultures. viral growth was confirmed by titration of the passage material in other susceptible host systems. nineteen viruses demonstrated good growth in c6/36 cells: coxsackievirus group a type 10 and group b types 2, 3, 4, and 5; enterovirus 69; mumps virus; poliovirus types 1 to 3; reovirus types 1 to 3; vaccinia virus; dengue virus type 2; eastern ... | 1987 | 3611315 |
| functional and antigenic domains of the dengue-2 virus nonstructural glycoprotein ns-1. | the gene coding for the nonstructural glycoprotein of dengue-2 virus was cloned, sequenced, and expressed in escherichia coli. there was about 70% conservation at the amino acid level with dengue serotypes 1 and 4 suggesting an important common function for this protein. conserved hydrophobic domains were found both before the amino-terminus and at the carboxy-terminus, consistent with transmembrane roles. evidence for at least partial translocation of ns-1 through the inner membrane of e. coli ... | 1988 | 2964755 |
| detection of dengue virus type 2 in aedes albopictus by nucleic acid hybridization with strand-specific rna probes. | a molecular hybridization technique with radiolabeled, strand-specific rna probes was developed to detect dengue virus type 2 rna in pools of infected aedes albopictus mosquitoes. one infected mosquito in a pool of 25 could be detected, corresponding to a dengue virus type 2 titer of 2.75 log10 50% tissue culture infectious doses. | 1988 | 3281980 |
| variation in dengue type 2 viruses isolated in bangkok during 1980. | dengue type-2 viruses isolated in metropolitan bangkok during 1980 (bangkok/80) were characterized by oligonucleotide fingerprinting, restriction enzyme (re) mapping and antigenic analysis using monoclonal antibody probes. of 10 isolates analysed by oligonucleotide fingerprinting, nine were very closely related, showing 72.5% to 91.4% oligonucleotide homology. one isolate (d80-141) produced a distinctly different fingerprint (55.7% to 58.0% homology) and was less related to other bangkok/80 deng ... | 1988 | 3351477 |
| evidence that maternal dengue antibodies are important in the development of dengue hemorrhagic fever in infants. | to establish the role of maternal dengue-specific antibodies in the development of dengue hemorrhagic fever and dengue shock syndrome caused by dengue 2 virus in infants, we examined sera from mothers of infants and toddlers with dengue hemorrhagic fever or dengue shock syndrome and mothers of infants with pyrexia of unknown origin. the mean titers of hemagglutination inhibition, neutralization, and infection-enhancing activities against dengue 2 virus were not statistically different among the ... | 1988 | 3354774 |
| partial nucleotide sequence and deduced amino acid sequence of the structural proteins of dengue virus type 2, new guinea c and puo-218 strains. | the nucleotide sequence and the deduced amino acid sequence for the genes encoding the structural proteins of two strains of dengue virus type 2 (den-2) were determined from cdna clones. the genes for c, prm(m) and e proteins were sequenced for the prototype den-2 virus, the new guinea c strain. also sequenced were the prm(m) and e genes of puo-218. this strain of den-2 was isolated during 1980 in bangkok and had received a limited number of laboratory passages. comparisons of the newly determin ... | 1988 | 3385407 |
| dengue 2 virus envelope protein expressed by a recombinant vaccinia virus fails to protect monkeys against dengue. | a cdna copy of the dengue (den) 2 virus genome region encoding the virion capsid, membrane and envelope structural proteins has been inserted into vaccinia virus (vv) dna under the control of its 11k late promoter. the den-2 envelope protein was expressed and processed in cells infected with the vv recombinant (vv/d2s). no den-2 virus antibody response was detected in mice, hamsters or monkeys vaccinated with vv/d2s. furthermore, a viraemia was observed in recombinant-vaccinated monkeys after ch ... | 1988 | 3404119 |
| synergistic interactions of anti-ns1 monoclonal antibodies protect passively immunized mice from lethal challenge with dengue 2 virus. | non-neutralizing, serotype-specific anti-ns1 monoclonal antibodies partially protected passively immunized mice from lethal dengue 2 virus intracerebral challenge. there was no apparent correlation between complement-fixing activity and protective capacity among individual anti-ns1 monoclonal antibodies. immunization with specific combinations of non-protective or partially protective antibodies resulted in prolonged survival or reduced mortality. solid protection, equal to that achieved after i ... | 1988 | 3404125 |
| nucleotide sequence of dengue 2 rna and comparison of the encoded proteins with those of other flaviviruses. | we have determined the complete sequence of the rna of dengue 2 virus (s1 candidate vaccine strain derived from the pr-159 isolate) with the exception of about 15 nucleotides at the 5' end. the genome organization is the same as that deduced earlier for other flaviviruses and the amino acid sequences of the encoded dengue 2 proteins show striking homology to those of other flaviviruses. the overall amino acid sequence similarity between dengue 2 and yellow fever virus is 44.7%, whereas that betw ... | 1988 | 2827375 |
| evidence that the mature form of the flavivirus nonstructural protein ns1 is a dimer. | evidence is presented which indicates that the dengue-2 virus nonstructural protein ns1 (soluble complement fixing antigen) exists in infected bhk and mosquito cell cultures as part of a stable oligomer. identification of the dissociation products of the isolated oligomer and comparison of the number of n-linked glycans in native and denatured ns1 is consistent with the idea that the high-molecular-weight form of ns1 is a homodimer. by analyzing lysates of bhk cells infected with st. louis encep ... | 1988 | 2827377 |
| protection of mice against japanese encephalitis virus by passive administration with monoclonal antibodies. | we have identified and characterized nine antigenic epitopes on the e envelope of japanese encephalitis virus (jev) by using mab. passive administration of most of the anti-jev mab protected mice from i.v. challenge with 1.5 x 10(3) plaque-forming units of jev, jagar-01 strain. some mab, which possess high neutralization activity in vitro, showed high protection, and jev-specific n mab 503 was found the most protective. even an injection of 2.5 micrograms/mouse of mab 503 protected all mice from ... | 1988 | 2460542 |
| serologically defined linear epitopes in the envelope protein of dengue 2 (jamaica strain 1409). | antisera from dengue patients and dengue virus infected rabbits recognized octapeptides corresponding to linear amino acid sequences in the envelope protein of dengue 2 (jamaica 1409). although no peptide was recognized by sera from all dengue infected hosts, two peptides (216lplpwlpg223 and 448fsgvswtm455) were recognized by sera from all dengue 2 infected rabbits. one of these 448fsgvswtm455 was also recognized by sera from both the dengue 2 patients tested. no peptides were identified which r ... | 1989 | 2473720 |
| dengue virus-specific human t cell clones. serotype crossreactive proliferation, interferon gamma production, and cytotoxic activity. | the severe complications of dengue virus infections, hemorrhagic manifestation and shock, are much more commonly observed during secondary infections caused by a different serotype of dengue virus than that which caused the primary infections. it has been speculated, therefore, that dengue hemorrhagic fever (dhf) and dengue shock syndrome (dss) are caused by serotype crossreactive immunopathological mechanisms. we analyzed clones of dengue serotype crossreactive t lymphocytes derived from the pb ... | 1989 | 2475573 |
| [antigen signature analysis of dengue-2 viruses strains in hainan china]. | in this paper, we try to define the extent of antigenic variation between dengue-2 viruses from hainan province, which had been isolated over a period of 3 years (1985-1987). the dengue-2 viruses were compared with the prototype new guinea b strain and were subjected to antigen signature analysis. eight strains of dengue-2 virus were analyzed by three monoclonal antibodies: flavivirus group, subcomplex dengue-2 type-specific reactive epitopes, over a range of antigen concentration. five out of e ... | 1989 | 2479176 |
| stimulation of plasminogen activator inhibitor activity in human monocytes infected with dengue virus. | human monocytes, in an essentially serum-free culture medium, were infected with dengue 2 virus in the presence of sub-neutralizing concentrations of antibody. changes in procoagulant activity (pca), in the plasminogen activator urokinase (uk), and the plasminogen activator inhibitor-2 (pai-2) were quantitated. one day after exposure to dengue virus, the cell-associated pai-2 activity in the infected monocytes was 562 +/- 9 mu/10(6) cells (mean +/- se) compared to 206 +/- 56 mu/10(6) cells for u ... | 1989 | 2492775 |
| effect of dengue virus on procoagulant and fibrinolytic activities of monocytes. | some of the fibrinolytic and coagulation enzymes that monocytes produce are urokinase, a plasminogen activator (pa); a pa-specific inhibitor (pai); and procoagulant activity (pca), which has been characterized as tissue factor. dengue infection in vivo is restricted to monocytes; however, it is unknown if dengue-infected monocytes undergo alterations in the production of pa, pai, and pca. this issue was addressed in studies in which monocytes were infected in vitro with dengue 2 virus in serum-f ... | 1989 | 2501855 |
| newly synthesized dengue-2 virus nonstructural protein ns1 is a soluble protein but becomes partially hydrophobic and membrane-associated after dimerization. | in a previous paper (g. winkler, v. b. randolph, g. r. cleaves, t. e. ryan, and v. stollar, 1988, virology 162, 187-196) we showed that the newly synthesized dengue-2 virus nonstructural protein, ns1, exists briefly as a monomer and then undergoes dimerization. we demonstrate here that the dimerization of ns1 is associated with a change in hydrophobicity and sedimentability of this protein. newly synthesized monomeric ns1 is a hydrophilic and water-soluble protein which cannot be pelleted at 75, ... | 1989 | 2525840 |
| genetic variation and microevolution of dengue 2 virus in southeast asia. | dengue 2 (den 2) virus strains collected from dengue hemorrhagic fever (dhf) patients and aedes aegypti mosquitoes in thailand, burma, and vietnam over a 25-year period have been analyzed by computer assisted t1-rnase-resistant oligonucleotide fingerprinting. fifty-seven den 2 virus strains of the thailand topotype were separated into four major clusters by phylogenetic analysis of 97 unique oligonucleotides identified in a common well-resolved region of the fingerprints. similarities in the 57 ... | 1989 | 2529700 |
| [transmission cycles of arboviruses in madagascar]. | some arboviruses are highly pathogenic for men or animals. arboviruses epidemiological patterns in madagascar were determined by entomological, serological, and virological surveys. we listed potentials arboviruses vectors in madagascar. entomological results generated by us during five years, as well as prior to, are shown. we caught more than 150,000 hematophagous arthropods, belonging to 107 species at least. 3 of these species were new. 4183 inoculation pools were done. we studied serollogic ... | 1989 | 2576622 |
| nucleotide sequence of the envelope protein gene of a malaysian dengue-2 virus isolated from a patient with dengue haemorrhagic fever. | 1989 | 2587234 | |
| nucleotide sequence of the envelope protein gene of a malaysian dengue-2 virus isolated from a patient with dengue fever. | 1989 | 2587242 | |
| nucleotide sequence of the envelope protein gene of a malaysian dengue-2 virus isolated from a patient with dengue shock syndrome. | 1989 | 2587243 | |
| rapid chemical mapping of dengue virus variability using rna isolated directly from cells. | osmium tetroxide and hydroxylamine (in combination with piperidine) have previously been shown to cleave mismatched t and c bases, respectively, in dna.dna heteroduplexes. in this work we report that mismatched t and c bases were similarly cleaved in dna.rna heteroduplexes of nucleic acids derived from different strains of dengue virus type 2. further, some matched t or c bases one or two bases from mismatches were also chemically reactive and thus cleavable as detected by minor bands. cleavages ... | 1989 | 2592522 |
| serological evidence of dengue fever among refugees, hargeysa, somalia. | epidemics of a malaria-like illness affected several thousand residents of the dam camp, a refugee camp near hargeysa in somalia, during 1985, 1986, and 1987. the disease was characterized by fever, chills, sweats, headache, back and joint pains for as long as 10 days in some patients. blood smears from acutely ill patients were negative for malaria. of 28 acute and 10 convalescent sera tested by the indirect fluorescent antibody (ifa) and by the hemagglutination inhibition (hi) tests, all were ... | 1989 | 2600591 |
| detection of some dengue-2 virus antigens in infected cells using immuno-microscopy. | immunoelectron microscopy was used to detect the distribution of some dengue-2 virus proteins in infected vero and aedes albopictus (c6/36) cells. it was found that the envelope protein (gp 60) was located in clumps on the surface of plasma membrane, and accumulated very little in the infected cytoplasm. however no envelopment of dengue-2 virus nucleocapsids through the plasma membranes was observed. in contrast, the ns 3 (p 67) protein was distributed throughout the whole cytoplasm. no specific ... | 1989 | 2650656 |
| the reemergence of dengue in china. | in 1978, dengue was reported in china for the first time in 32 years. since then, epidemics involving hundreds of thousands of people have occurred in guangdong and guangxi provinces and on hainan island. these epidemics were caused by all four types of dengue virus. aedes aegypti was the vector in coastal areas, while aedes albopictus was the vector in inland regions. during these epidemics, case rates were very high (greater than 50%) in some areas. case-fatality rates were generally less than ... | 1989 | 2665017 |
| an improved method for the detection of peroxidase-conjugated antibodies on immunoblots. | a sensitive method for the detection of antigen-antibody complexes on immunoblots is described which employs a modified substrate for peroxidase-conjugates. dengue virus proteins were chosen for study and were separated by sds-page followed by electrophoretic transfer onto sheets of nitrocellulose. virus-specific antigens bound to the nitrocellulose paper were then probed with both mouse monoclonal antibodies and human convalescent sera. antigen-antibody complexes were detected with anti-species ... | 1989 | 2668315 |
| sequence analysis of cloned dengue virus type 2 genome (new guinea-c strain). | sequences totalling 5472 nucleotides (nt) from four complementary dna (cdna) clones of the dengue virus type 2 (den-2) rna (new guinea strain, ngs-c) have been reported previously [yaegashi et al., gene 46 (1986) 257-267; putnak et al., virology 163 (1988) 93-103]. this report describes the complete nucleotide sequence, with the exception of about 7 nt at the 5'-noncoding region, of this rna genome derived from several cdna clones. it is 10,723 nt in length and contains a single long open readin ... | 1989 | 2714651 |
| definition of the carboxy termini of the three glycoproteins specified by dengue virus type 2. | the carboxy termini of the three glycoproteins (prm, e, and ns1) specified by dengue virus type 2 (den-2) were determined. the glycoproteins were radiolabeled with selected amino acids chosen following analysis of the deduced amino acid sequence of the polyprotein and then digested with carboxypeptidase a. the pattern of release of radioactive amino acids enabled definition of the carboxy termini. in addition, the amino terminus of ns2a was determined by edman degradation of the radiolabeled pro ... | 1989 | 2741348 |
| [arbovirus transmission cycles in madagascar]. | some arboviruses are highly pathogenic for men or animals, arboviruses epidemiological patterns in madagascar were determined by entomological, serological, and virological surveys. we listed potentials arboviruses vectors in madagascar. entomological results generated by us during five years, as well as prior to, are shown. we caught more than 150,000 hematophagous arthropods, belonging to 107 species at least. 3 of these species were new. 4183 inoculation pools were done. we studied serologica ... | 1989 | 2751393 |
| [comparative study of dengue 2 virus replication and isoenzymes in two strains of aedes aegypti]. | this study, dealing with two strains of aedes aegypti from vietnam and french guiana, shows the variability of the genes coding for 11 isoenzymatic systems and the replication of the dengue 2 virus in parenterally infected mosquitoes. slight differences are observed in the characteristics of viral replication. no clear correlation is shown with enzymatic patterns which appear widely different from one strain to the other with four of the enzymes studied. | 1990 | 2085909 |
| isolation of dengue virus type 2 in rio de janeiro. | 1990 | 2087163 | |
| immunization of mice with recombinant vaccinia virus expressing authentic dengue virus nonstructural protein ns1 protects against lethal dengue virus encephalitis. | the protective immunity conferred by a set of recombinant vaccinia viruses containing the entire coding sequence of dengue virus type 4 nonstructural glycoprotein ns1 plus various flanking sequences was evaluated by using a mouse encephalitis model. mice immunized with recombinant vns1-ns2a, which expresses authentic ns1, were solidly protected against intracerebral dengue virus challenge. however, mice immunized with recombinants vns1-15%ns2a and vrsvg/ns1-15%ns2a, which express aberrant forms ... | 1990 | 2143542 |
| in vitro processing of dengue virus type 2 nonstructural proteins ns2a, ns2b, and ns3. | we have tested the hypothesis that the flavivirus nonstructural protein ns3 is a viral proteinase that generates the termini of several nonstructural proteins by using an efficient in vitro expression system and monospecific antisera directed against the nonstructural proteins ns2b and ns3. a series of cdna constructs was transcribed by using t7 rna polymerase, and the rna was translated in reticulocyte lysates. the resulting protein patterns indicated that proteolytic processing occurred in vit ... | 1990 | 2143543 |
| dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections. | twenty three human mononuclear cell lines including ten myelomonocytic cell lines, eight b cell lines and five t cell lines, were examined to determine whether they could be infected with dengue-2 virus. all the cell lines were infected with dengue-2 virus as determined by immunofluorescent staining and by virus titration of culture supernatant fluids. k562, jiyoye and jurkat, respectively, showed the highest percentage of infected cells of these myelomonocytic, b and t cell lines. antibody to d ... | 1990 | 2178591 |
| nucleotide sequence of the envelope glycoprotein gene of a dengue-2 virus isolated during an epidemic of benign dengue fever in tonga in 1974. | 1990 | 2216784 | |
| variation among strains of aedes aegypti in susceptibility to oral infection with dengue virus type 2. | we compared 18 aedes aegypti strains for oral susceptibility to dengue virus type 2 (den-2) using a feeding protocol in which all parameters remained constant, including the titer of the infectious bloodmeal. for most strains, no significant variation between replicates was observed. comparisons between pairs of strains showed variation of different degrees, and allowed us to characterize the strains with respect to their oral susceptibility to den-2. | 1990 | 2221225 |
| expression of the structural proteins of dengue 2 virus and yellow fever virus by recombinant vaccinia viruses. | vaccinia virus recombinants were constructed which contained cdna sequences encoding the structural region of dengue 2 virus (pr159/s1 strain) or yellow fever virus (17d strain). the flavivirus cdna sequences were expressed under the control of the vaccinia 7.5k early/late promotor. cultured cells infected with these recombinants expressed immunologically reactive flavivirus structural proteins, precursor prm and e. these proteins appeared to be cleaved and glycosylated properly since they comig ... | 1990 | 2260923 |
| antibodies to dengue 2 virus e-glycoprotein synthetic peptides identify antigenic conformation. | eighteen synthetic peptides have now been prepared from dengue (den) 2 virus, representing 80% of the extra-membranal domain of the den envelope (e)-glycoprotein. these peptides were selected based upon our previous results with murray valley encephalitis (mve) virus, computer analysis of the den virus sequence, and the predicted e-glycoprotein secondary structure. six of these peptides were "structural" peptides derived from either nonlinear amino acid sequences or predicted disulfide bridge-st ... | 1990 | 2371772 |
| detection of dengue viral rna in mosquito vectors by mixed phase and solution hybridization. | a mixed phase hybridization technique was developed to detect dengue virus type 2 (den-2) rna in pools of infected aedes albopictus mosquitoes using radiolabelled rna probes. this technique used a guanidine thiocyanate extraction procedure to simplify analyte preparation. the probes contained sequences complementary to portions of the ns-1 or ns-5 genes of the den-2 viral genome. one infected mosquito in a pool of 25 could be detected in approximately 48 h. rnas from den serotypes 1-4 were extra ... | 1990 | 2402251 |
| dengue-2 virus in kenya. | 1990 | 1977046 | |
| processing, secretion, and immunoreactivity of carboxy terminally truncated dengue-2 virus envelope proteins expressed in insect cells by recombinant baculoviruses. | two recombinant baculoviruses were constructed by inserting via the transfer vector pacym1 the genes coding for the structural proteins of dengue (den)-2 virus downstream from the polyhedrin promoter of autographa californica nuclear polyhedrosis virus. the two recombinants differed in truncation of 26 and 71 amino acids, respectively, in the carboxy-terminal sequence of den-specific envelope (e) glycoprotein. recombinant den-2 e glycoproteins were processed and transported to the surface of spo ... | 1991 | 1984665 |
| outbreak of dengue fever in rural areas of parbhani district of maharashtra (india). | outbreak of dengue fever in chikalthana, pimpalgaon and waloor villages in parbhani district of maharashtra (india) were investigated. clinically, the illness was typical of dengue fever except for the absence of maculopapular rash. a total of 42 acute, 14 late acute, 73 convalescent and 19 sera from contacts were collected. of the 15 virus isolates, 12 were identified as dengue virus type 2 and 1 as dengue virus type 1. serological tests confirmed the etiological role of dengue virus in the out ... | 1991 | 2022403 |
| the first epidemic of dengue hemorrhagic fever in the people's republic of china. | the first epidemic of dengue in china associated with significant severe and fatal hemorrhagic disease which met the world health organization case definition occurred on hainan island in 1985-1986. the epidemic began in zhan county in september 1985, spread throughout the coastal areas, and ultimately involved 13 counties and cities of the island in 1986. the mosquito vector was aedes aegypti. the morbidity associated with dengue infection on hainan island was 1,913 per 100,000 residents, with ... | 1991 | 2042704 |
| [application of the polymerase chain reaction (pcr) to diagnosis in virology]. | recall of the principle of the pcr and of its advantages: rapidity, possible automatization, high sensitivity allowing the detection of minute amounts of infectious agents, specificity depending on that of the primers used for the detection of the amplified product. but this technic has to be used with care to avoid any contamination which might give false positive results and requires controls. this procedure would allow to analyze the evolution and the variability of infectious agents by their ... | 1991 | 1819420 |
| the sensitivity of cell-associated dengue virus proteins to trypsin and the detection of trypsin-resistant fragments of the nonstructural glycoprotein ns1. | extracts of vero cells infected with dengue virus type 2 were digested by trypsin in the presence and absence of detergents. the experiments were designed to test the models proposed for flavivirus translation in which the glycoproteins prm, e, and ns1 are inserted into the endoplasmic reticulum of the cell, whereas certain other nonstructural proteins are not. viral polypeptides were detected by the use of radiolabel, by immunoprecipitation, or by immunoblotting. the results obtained for ns3 an ... | 1991 | 1824904 |
| expression of the ns1 gene of dengue virus type 2 using vaccinia virus. dimerisation of the ns1 glycoprotein. | a part of the genome of dengue virus type 2 spanning the coding region from the carboxy terminus of the envelope protein e to the beginning of the ns3 protein was expressed using recombinant vaccinia virus. additional constructs which contained open reading frames terminating within the ns1 or ns2a genes were also expressed. ns1 dimers were formed by extended ns1 molecules containing 61 amino acids of ns2a. no dimers were detected when ns1 was shortened by 79 amino acids at its carboxy terminus. | 1991 | 1826827 |
| ns 1 gene sequences from eight dengue-2 viruses and their evolutionary relationships with other dengue-2 viruses. | the nucleotide sequences of the ns 1 genes from five thai and three sri lankan dengue-2 viruses were determined by sequencing the viral rna using synthetic oligonucleotide primers. the results were shown to be similar to four published dengue-2 ns 1 sequences and the classification of these genes was compared with the one obtained for the envelope genes of the same viruses. the classification was similar and showed that the thai isolates could be divided into two separate groups and that the sri ... | 1991 | 1829885 |
| production of dimer-specific and dengue virus group cross-reactive mouse monoclonal antibodies to the dengue 2 virus non-structural glycoprotein ns1. | a panel of mouse monoclonal antibodies (mabs) raised against the non-structural glycoprotein ns1 of dengue 2 virus (pr159) was studied for cross-reactivity with the ns1 protein of other dengue virus serotypes and other members of the flaviviridae using immunoblotting. most of the 35 anti-ns1 mabs were found to be specific for dengue 2 virus ns1 (some of which were specific for the native, dimeric form of this protein), but others were found to cross-react within the dengue virus group. this latt ... | 1991 | 1849979 |
| dengue virus premembrane and membrane proteins elicit a protective immune response. | we have constructed recombinant vaccinia viruses that express the premembrane (pre-m), membrane (m), or the cleaved, residual portion of pre-m (non-m) proteins of dengue 4 virus, or the pre-m, non-m, or envelope (e) proteins of dengue 2 virus, to evaluate their ability to induce protective immunity in mice. cells infected with these recombinants make proteins of expected size. mice immunized with recombinants expressing dengue 4 pre-m or m were protected against subsequent dengue 4 encephalitis ... | 1991 | 1926792 |
| analysis of inheritance of oral susceptibility of aedes aegypti (diptera: culicidae) to dengue-2 virus using isofemale lines. | isofemale lines were compared to determine possible genetic variation in oral susceptibility within the fare strain of aedes aegypti (l.) to dengue-2 (den-2) virus. three groups of 12 isofemale lines each were tested statistically using the sas catmod procedure of analysis of variance. the "isofemale line" effect was highly significant, demonstrating genetic variability in oral susceptibility among females. the length of time eggs were stored before hatching influenced the oral susceptibility of ... | 1991 | 1941912 |
| detection of cattle infected with bovine viral diarrhea virus using nucleic acid hybridization. | a ribonucleic acid (rna) hybridization assay to identify cattle infected by bovine viral diarrhea virus (bvdv) is described. the rna probe was derived from the coding region at the 3' end of the genome of the nadl strain of bvdv. total rna from infected cell cultures or peripheral blood leukocytes from suspect animals was extracted and applied to nylon membranes with a slot blot apparatus. peripheral blood leukocytes were tested concurrently for bvdv by virus isolation. the results of hybridizat ... | 1991 | 1645592 |
| obligatory role of ca2+ in the cytotoxic activity of dengue virus-induced cytotoxin. | the role of calcium ions (ca2+) in the cytotoxic activity of the dengue type 2 virus (dv)-induced macrophage (m phi) cytotoxin (cf2) was investigated in the present study. the findings show that cf2 prepared in ca(2+)-free medium had no cytotoxic activity on normal mouse spleen cells suspended in ca(2+)-free medium but killed the cells suspended in the medium with ca2+. substitution with calcium chloride restored the cytotoxic activity of cf2 the optimal dose being 10(-4) m concentration. cf2 in ... | 1991 | 1653589 |
| detection of stable secondary structure at the 3' terminus of dengue virus type 2 rna. | the 3'-terminal sequences of flavivirus genomes within approx. 100 nucleotides (nt) have been suggested to have a highly conserved secondary structure, as based on the known nt sequence data and free-energy calculations using computer programs. to test the existence of a secondary structure in solution, we devised a strategy to generate truncated rna molecules from about 0.3-1.4 kb in length, having the same polarity and nt sequence as dengue virus type 2 (den-2) rna (new guinea-c strain). when ... | 1991 | 1660836 |
| a serological study of japanese encephalitis virus infections in northern peninsular malaysia. | this study describes the status of viral encephalitis in perak, malaysia during the year 1990. in addition, 14 cases selected from penang and perak during the years 1989 and 1990 are presented, with data showing titers of neutralizing antibodies against japanese encephalitis virus (jev) and dengue 2 virus, titers of antibodies against jev and dengue virus antigens as determined by deia, and a comparison of these with the presence of igm to jev and dengue virus. these data show that there probabl ... | 1991 | 1667957 |
| construction of intertypic chimeric dengue viruses by substitution of structural protein genes. | dengue virus contains an 11-kilobase positive-strand rna genome that codes for, in one open reading frame, three structural proteins (capsid, premembrane, and envelope), followed by seven nonstructural proteins. the structural protein genes of a full-length cdna clone of type 4 dengue virus were replaced with the corresponding genes of dengue 1 or dengue 2 to create intertypic chimeric cdna. the rna transcripts made from these templates were infectious when transfected into permissive cells in c ... | 1991 | 1682924 |
| processing of nonstructural proteins ns4a and ns4b of dengue 2 virus in vitro and in vivo. | the production, from polyprotein precursors, of two hydrophobic nonstructural proteins of dengue 2 (den2) virus, ns4a and ns4b, was analyzed both in cell-free systems and in infected cells. in den2-infected cells, ns4b is first produced as a peptide of apparent size 30 kda; ns4b is then post-translationally modified, in an unknown way, to produce a polypeptide of apparent size 28 kda. the rate and extent of ns4b modification was found to be cell-dependent; in bhk cells the half-time for the conv ... | 1991 | 1683727 |
| replication of dengue virus type 2, brazilian strain, in mosquito cell cultures. | 1991 | 1688183 | |
| breakdown of the blood-brain barrier during dengue virus infection of mice. | a breakdown of the blood-brain barrier occurred in mice inoculated intracerebrally (i.c.) or intraperitoneally (i.p.) with dengue virus type 2 (den2). this resulted in leakage of protein-bound evans blue dye and 51cr-labelled erythrocytes into the brain tissue. the leakage increased with time after infection and coincided with an increase of a den2-induced cytokine, the cytotoxic factor (cf), in the spleens of such mice. the titres of virus in the brain increased exponentially in i.c. inoculated ... | 1991 | 1707947 |
| processing of dengue virus type 2 structural proteins containing deletions in hydrophobic domains. | the 5' end of the genome of the dengue virus type 2 encoding the structural proteins was expressed using recombinant vaccinia virus. three additional recombinants derived by deletion of selected dengue sequences within the parental construct were also expressed. they were designed to assess the role of hydrophobic domains in the processing of the viral polyprotein in intact cells. the first construct contained a deletion of nucleotides encoding most of the c protein; nucleotides encoding the hyd ... | 1992 | 1729986 |
| nuclear localization of dengue 2 virus core protein detected with monoclonal antibodies. | anti-dengue 2 virus core protein monoclonal antibodies (mabs) reacted with antigens in the cytoplasm and in, or on, the nucleus of dengue 2 and dengue 4, but not dengue 1, dengue 3, kunjin or murray valley encephalitis virus-infected cells. these mabs also reacted with the core protein from dengue 1, 2 and 4 virions in western blots. the antigens detected by these mabs could not be detected in uninfected or heat-shocked cells, but were first detected in infected cells approximately 32 h post-inf ... | 1992 | 1279106 |
| comparison of a dengue-2 virus and its candidate vaccine derivative: sequence relationships with the flaviviruses and other viruses. | a comparison of the sequence of the dengue-2 16681 virus with that of the candidate vaccine strain (16681-pdk53) derived from it identified 53 of the 10,723 nucleotides which differed between the strains. nucleotide changes occurred in genes coding for all virion and nonvirion proteins, and in the 5' and 3' untranslated regions. twenty-seven of the nucleotide changes resulted in amino acid alterations. the greatest amino acid sequence differences in the virion proteins occurred in prm (2.20%; 2/ ... | 1992 | 1312269 |
| envelope protein sequences of dengue virus isolates th-36 and th-sman, and identification of a type-specific genetic marker for dengue and tick-borne flaviviruses. | complementary dnas were synthesized from the envelope protein genes of two isolates of dengue virus (th-36 and th-sman, previously suggested as possible dengue virus type 5 and dengue virus type 6 respectively) and amplified by the polymerase chain reaction using sense and antisense primers designed from conserved dengue virus gene sequences. the amplified cdna clones were sequenced in both directions by double-stranded dideoxynucleotide sequencing. alignment with published dengue virus sequence ... | 1992 | 1339466 |
| dengue 2 virus enhancement in asthmatic and non asthmatic individual. | during the 1981 dengue hemorrhagic fever/dengue shock syndrome (dhf/dss) cuban epidemic, bronchial asthma (ba) was frequently found as a personal or family antecedent in dengue hemorrhagic fever patients. considering that antibody dependent enhancement (ade) plays an important role in the etiopathogenic mechanism of dhf/dss, we decide to study the dengue 2 virus (d2v) capability of replication in peripheral blood leukocytes (pbl) from asthmatic patients and healthy persons. in 90% of asthmatic p ... | 1992 | 1343671 |
| use of recombinant fusion proteins and monoclonal antibodies to define linear and discontinuous antigenic sites on the dengue virus envelope glycoprotein. | sixteen overlapping fragments of the dengue-2 virus envelope (e) protein, expressed as trpe-e fusion products in escherichia coli, were used to map the epitopes defined by a panel of 20 monoclonal antibodies (mabs) by immunoblotting. using this technique, the amino acid sequence of six antigenic domains on the e protein was characterized. nonneutralizing mabs were found to define either linear-specific, subcomplex-specific (amino acids 22-58), and complex-specific (amino acids 304-332) epitopes ... | 1992 | 1372140 |
| amplification of dengue 2 virus ribonucleic acid sequence using the polymerase chain reaction. | the polymerase chain reaction (pcr) has been adapted to the amplification of dengue type 2 virus (den2) nucleic acid sequences. a pair of 20-mer oligonucleotides were designed and synthesized based on conserved sequence blocks of den2 strains isolated from different geographical areas. rna samples were prepared from two den2 strains, prototype new guinea c (ngc) and local isolate hainan 98 (hn98). the reverse transcription step was performed for cdna synthesis before the standard pcr procedures. ... | 1992 | 1381844 |
| processing and localization of dengue virus type 2 polyprotein precursor ns3-ns4a-ns4b-ns5. | processing of dengue virus type 2 polyprotein precursor ns3-ns4a-ns4b-ns5 could be mediated by the catalytically active ns3 protease domain and ns2b in trans at the dibasic sites ns3-ns4a and ns4b-ns5. subcellular localization of the unprocessed precursor ns3-ns4a-ns4b-ns5 showed that it was confined to a distinct subcellular organelle in the cytoplasm, which was distinct from the distribution of the mature ns5. | 1992 | 1433530 |
| geographical clusters of dengue virus type 2 isolates based on analysis of infected cell rna by the chemical cleavage at mismatch method. | genetic variation in 12 strains of dengue virus type 2, isolated from several epidemic areas in different years, was studied by chemical cleavage at mismatched cytosine in dna:rna heteroduplexes. end-labelled cdna probes derived from the e and ns2a genes of the new guinea c strain were hybridized to total rna extracted from cells infected by individual isolates. following modification of mismatched cytosine by hydroxylamine and nucleic acid strand cleavage by piperidine, the resulting fragments ... | 1992 | 1452634 |
| dengue haemorrhagic fever in children in delhi. | an epidemic of dengue haemorrhagic fever occurred in delhi during 1988. a total of 21 paediatric patients with dengue haemorrhagic fever/dengue shock syndrome were evaluated from september to november 1988. all the patients had fever, restlessness, ecchymotic spots and ascites. pleural effusion occurred in 19 patients (90%), and 18 (86%) exhibited each of the following: vomiting, thrombocytopenia, and haemoconcentration. hepatomegaly was observed in 15 patients (71%) and splenomegaly in three (1 ... | 1992 | 1568274 |
| dengue-2 virus isolation from humans during an epizootic in southeastern senegal in november, 1990. | 1992 | 1594790 | |
| mapping of a region of dengue virus type-2 glycoprotein required for binding by a neutralizing monoclonal antibody. | envelope glycoprotein e of flaviviruses is exposed at the surface of the virion, and is responsible for eliciting a neutralizing antibody (ab) response, as well as protective immunity in the host. in this report, we describe a method for the fine mapping of a linear sequence of the e protein of dengue virus type-2 (den-2), recognized by a type-specific and neutralizing monoclonal ab (mab), 3h5. first, an escherichia coli expression vector containing a heat-inducible lambda pl promoter was used t ... | 1992 | 1634111 |
| identification of helper t cell epitopes of dengue virus e-protein. | the t cell proliferative response to dengue 2 (jamaica) e-glycoprotein (495 amino acids) was analyzed in vitro using either killed virus or e-protein fragments or synthetic peptides. inactivated dengue virus stimulated dengue-specific lymph node (ln) cd4+t cell proliferation in balb/c (h-2d), c3h (h-2k) and dba/1 (h-2q) but not in c57bl/6 (h-2b) mice. moreover, ln cells from dengue-virus primed balb/c mice proliferated in vitro in response to three purified non-overlapping e-protein fragments ex ... | 1993 | 7683752 |
| detection of dengue-2 viral rna by reversible target capture hybridization. | a reversible target capture (rtc) sandwich hybridization technique has been developed for the detection of dengue-2 viral rna. the rtc is a form of sandwich hybridization that utilizes two probes: a poly(da)-tailed capture probe and a labeled detector probe. following hybridization of both probes to the analyte in solution, the poly(da)-tailed capture probe is used to selectively remove the hybrids by capture on oligo(dt)-coated paramagnetic beads. after elution from the beads, the presence of s ... | 1993 | 7902844 |
| [surveillance of the circulation of arbovirus of medical interest in the region of eastern senegal]. | a study about the circulation of arboviruses of medical interest in southeastern senegal was conducted from 1988 to 1991, during and around the periods of transmission. specific igm antibodies were detected by elisa test in human sera, as a sign of a recent infection within 2 to 5 months. the comparison of the serological igm results from human surveys in different villages, and the isolations of arboviruses from mosquitoes during the same period of time permitted a rapid and global evaluation o ... | 1993 | 8099299 |
| [yellow fever virus, dengue 2 and other arboviruses isolated from mosquitos, in burkina faso, from 1983 to 1986. entomological and epidemiological considerations]. | an arbovirus surveillance was carried out in burkina faso from 1983 to 1986. it was based on crepuscular catches of mosquitoes on human bait in some wooded areas and in one town. the total collection was 228 catches with an average of 8 men per catch. the total number of mosquitoes caught was 44,956 among which 32,010 potential vector of yellow fever; all these mosquitoes were analysed for arbovirology. in the south-western part of the country (region of bobo-dioulasso), surveillance was conduct ... | 1993 | 8102567 |
| absence of fc receptors on aedes albopictus c6/36 cells permissive for dengue virus 2. | 1993 | 8105669 | |
| phylogenetic relationships of dengue-2 viruses. | rna oligonucleotide fingerprinting studies on a large number of virus isolates previously demonstrated considerable genetic variation in isolates of dengue (den)-2 serotype. we report the entire envelope (e) glycoprotein gene and deduced amino acid sequences of 16 den-2 viruses and the phylogenetic relationships of these, plus 17 additional published den e gene sequences. comparison of den-2 e glycoprotein gene sequences revealed base substitutions scattered throughout the entire gene with as mu ... | 1993 | 8212556 |
| role of protein conformation in the processing of dengue virus type 2 nonstructural polyprotein precursor. | the dengue virus type-2 (den-2) genome is a positive-strand rna encoding a single polyprotein precursor, c-prm(m)-e-ns1-ns2a-ns2b-ns3-ns4a-ns4b- ns5, consisting of 3391 amino acids (aa). the n-terminal region of the polyprotein precursor, c-prm(m)-e, encodes the structural proteins and is processed cotranslationally by the host signal peptidase. the nonstructural region ns1-->ns5 is processed by the viral protease(s), as well as by the signal peptidase. a two-component viral protease consisting ... | 1993 | 8325506 |
| dengue virus-specific human cd4+ t-lymphocyte responses in a recipient of an experimental live-attenuated dengue virus type 1 vaccine: bulk culture proliferation, clonal analysis, and precursor frequency determination. | we analyzed the cd4+ t-lymphocyte responses to dengue, west nile, and yellow fever viruses 4 months after immunization of a volunteer with an experimental live-attenuated dengue virus type 1 vaccine (den-1 45az5). we examined bulk culture proliferation to noninfectious antigens, determined the precursor frequency of specific cd4+ t cells by limiting dilution, and established and analyzed cd4+ t-cell clones. bulk culture proliferation was predominantly dengue virus type 1 specific with a lesser d ... | 1993 | 8371350 |
| the effects of site-directed mutagenesis on the dimerization and secretion of the ns1 protein specified by dengue virus. | cdna of dengue virus type 2 encoding the glycoprotein ns1 was transiently expressed in cos cells using an sv40-based vector. to test the importance of selected regions of the protein in dimer formation, constructs were prepared which encoded amino acid changes at specific locations. six of the 12 cys residues in the ns1 protein were individually changed to ala. two amino acids in each of three hydrophilic and two hydrophobic areas of the protein were also changed to ala. the ability of these 11 ... | 1993 | 8389081 |
| synthesis of dengue virus rna in vitro: initiation and the involvement of proteins ns3 and ns5. | an assay for flavivirus rna-dependent rna polymerase activity in vitro was established using extracts of vero cells infected with dengue virus type 2 (den-2) or kunjin virus (kun). rna synthesis was initiated on a template of viral replicative form (rf) and rf was converted to the replicative intermediate (ri). the rna-dependent rna polymerase complex of den-2 utilised either den-2 or kun rf as template, and similarly the kun polymerase complex utilised either den-2 or kun rf template. in additi ... | 1993 | 8418788 |
| high-level expression of the japanese encephalitis virus e protein by recombinant vaccinia virus and enhancement of its extracellular release by the ns3 gene product. | recombinant vaccinia viruses expressing the prm and e genes of the japanese encephalitis virus (jev) were constructed by use of synthetic promoters. while the recombinant virus moj6-sl, with an optimized vaccinia late-gene promoter, produced a 20-fold elevated level of e protein, as well as an 86-kda precursor protein in infected cells, no significant quantitative difference was detected between the extracellular or cell-surface e protein produced by moj6-sl and those produced by moj6 with the 7 ... | 1993 | 8421896 |
| immunoreactivity and protective effects in mice of a recombinant dengue 2 tonga virus ns1 protein produced in a baculovirus expression system. | the double-stranded replicative form of dengue 2 virus (den-2) rna (tonga strain) was used as a substrate to produce dna clones of the ns1-ns2a genes via reverse transcriptase synthesis of full length cdna followed by polymerase chain reaction amplification of the ns1-ns2a region. products were cloned into ptz18r for sequencing and into baculovirus for expression studies. the deduced amino acid sequence of the ns1-ns2a was almost identical to that of the s1 attenuated strain of den-2 puerto rico ... | 1993 | 8423452 |