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serological prevalence of infectious bursal disease in madhya pradesh and maharashtra. 1979231575
further morphological characterization and structural proteins of infectious bursal disease virus.an outer layer surrounding the capsid of infectious bursal disease virus was evident from electron micrographs of intact virus particles having diameters of 62 to 63 nm. the capsid was found to be composed of large morphological units or capsomeres, measuring about 12 nm in diameter. the architecture of the capsid appears to be that of t = 3 symmetry, with a probable 32 morphological units by rotational enhancement of image detail. structural proteins of infectious bursal disease virus consist o ...1979232182
response of susceptible versus immune chicks to killed, live-modified, and wild infectious bursal disease virus vaccines.modified infectious bursal disease virus (ibdv) administered ocularly to either susceptible or passively immune chicks did not induce protection against bursal atrophy by wild ibdv, while intramuscular (im) or intrabursal (ib) injection protected susceptible chickens. no protection was obtained in passively immune chickens vaccinated im or ib. susceptible and passively immune chickens vaccinated with a single dose of killed-virus suspension (kvs) did not become resistant to wild ibdv challenge. ...1979232653
characterization of immunosuppression in chickens by infectious bursal disease virus.chickens were infected with infectious bursal disease (ibd) virus in ovo or at different times posthatching to 6 weeks of age. the b- and t-cell responses in the lymphoid tissues and blood were examined sequentially to 8 weeks of age by using indirect immunofluorescence. the proportion of b-cells was consistently lower in infected birds than in controls, especially in chicks infected as embryos or at 1 day old. the proportion of t-cells increased following these early infections but was slightly ...1979232660
interaction of infectious bursal disease and coccidiosis in layer replacement chickens.during a floor-pen study on anticoccidial programs for layer-replacement pullets, there was a natural outbreak of infectious bursal disease (ibd). the interaction of coccidiosis and ibd was characterized by: excessive deaths from coccidiosis; and apparent failure of some anticoccidial drug treatments. immunity to coccidiosis was not blocked by the ibd infection, although immunity was less in some treatments than would be expected with the degree of coccidiosis exposure.1979232661
immunosuppressive effect of infectious bursal disease virus on vaccination against infectious bronchitis.groups of broiler chicks hatched with parental antibodies to infectious bursal disease virus (ibdv) and infectious bronchitis virus (ibv) were vaccinated against ibv at 1 day of age via the oculonasal routes and inoculated with virulent ibdv at 1, 5, 10, 15 or 20 days of age. while the non-ibdv inoculated birds were solidly immune against ibv challenge at an age of 29 days, immunity in the ibdv infected birds was depressed. this depression, which was most serious in the birds ibdv inoculated at ...197918770430
isolation of infectious bursal disease virus from turkeys.a virus, which is morphologically identical and antigenically related to two previously known isolates of infectious bursal disease (ibd) virus, was isolated from pooled faeces of 6-week-old turkeys with diarrhoea. it is concluded that this virus, designated ty89, is an isolate of ibd virus. the isolation of ty89 was heavily dependent upon the use of electron microscopy and the immunofluorescence technique. antibody to ty89 virus was detected by both indirect immunofluorescence and serum neutral ...197918770446
detection of viruses in avian faeces by direct electron microscopy.a total of 151 specimens of turkey and chicken faeces and intestinal contents were examined for the presence of viruses by electron microscopy. viruses were detected in 48 of these specimens (32%). the most frequently observed viruses were rotaviruses and enterovirus-like particles. rotavirus infection was associated with outbreaks of diarrhoea in turkeys, but symptomless rotavirus infection was seen in broiler chickens. adeno-viruses and infectious bursal disease virus were also observed in tur ...197918770449
evaluation of a vaccine against infectious bursal disease in field trials.it has been established in field trials that the mutant strain cu-1m of the infectious bursal disease virus can be used for vaccination against the disease. vaccination does not cause clinical signs or significant pathological alterations in the bursa of fabricius, and consequently it is not accompanied by immunosuppressive effects. in the proposed vaccination scheme maternally derived antibodies do not interfere with the establishment of immune protection in young chickens.197918770462
effects of infectious bursal disease virus and reticuloendotheliosis virus infection of chickens on the incidence of marek's disease and on local tumour development of the non-producer jmv transplant.dual infection of chickens with the hprs-16 or hprs-b14 strains of marek's disease (md) virus and non-transforming reticuloendotheliosis virus (rev-c) was shown not to affect significantly the incidence of md or the incidence of md lymphomas. simultaneous infection of chickens with a pathogenic strain of infectious bursal disease virus (ibdv) and the hprs-b 14 strain of md virus resulted in a consistently reduced incidence of md and lengthening of the latent period, but the incidence of md lymph ...198018770244
prevention of infectious bursal disease (ibd) by feeding ibd virus-immune bovine colostrum.addition of 5% freeze-dried ibd virus (ibdv)-immune bovine colostrum to the diet of chickens prevented infection when housed in an ibdv-contaminated environment. four of five chickens receiving 2.5%, and all chickens receiving 0.5% or no ibdv-immune colostrum were infected. no interference with development of an active immunity was found in chickens receiving 5% ibdv-immune colostrum in their diet and vaccinated intramuscularly with modified ibdv.198015615054
investigations on live vaccines against infectious bursal disease of chicks.four live virus vaccines against infectious bursal disease (ibd) were studied with regard to their safety, immune response and applicability. none of the vaccines caused clinical symptoms or had an adverse impact on bodyweight. differences between these vaccines were observed in their effect on the bursa/bodyweight ratio and the severity of the microscopical lesions of the bursa fabricii. the immunosuppressive effect of ibd vaccination at one day of age on the response to newcastle disease vacci ...19806243425
helix-coil transitions in double-stranded viral rna. fine resolution melting and ionic strength dependence.helix-coil transitions of double-stranded rna from reovirus and infectious bursal disease virus were measured optically in aqueous medium of different ionic strengths. in rna from reovirus four transitions and in rna from infectious bursal disease virus two transitions were resolved and evaluated quantitatively. the ionic strength dependence of their midpoint temperatures dtm/dlog[na+] were 13.5 +/- 0.3 degrees c for reovirus rna and 14.9 +/-0.7 degrees c for infectious bursal disease virus rna. ...19806243983
[diagnosis of gumboro disease]. 19806246675
significance of bursa of fabricius as target organ in infectious bursal disease of chickens.the highly virulent strain cu-1 of infectious bursal disease virus caused 100% mortality in 4-week-old specific pathogen-free chickens. in contrast, chickens infected after bursectomy did not become sick and only showed some discrete and transient necrosis in lymphatic tissues. however, these chickens contained infectious virus and, subsequently, produced specific antibodies. the virus concentrations in the organs studied reached their maximum 2 days postinfection, but were about 1,000 times low ...19806247275
studies on infectious bursal disease in chickens. 2. scoring microscopic lesions in the bursa of fabricius, thymus, spleen, and kidney in gnotobiotic and battery reared white leghorns experimentally infected with infectious bursal disease virus.during a 2 1/2 year study on the effect of experimentally-induced infectious bursal disease (ibd) in white leghorns, a system for scoring microscopic lesions was developed. microscopic changes in the bursa of fabricius, thymus, spleen, and kidney were studied to create a lesion score for each organ. no changes occurred in the cecal tonsil and few, if any, changes occurred in the liver. the system was useful in evaluating the severity of pathologic and inflammatory changes in each organ during th ...19806248848
passively transferred immunity to ibd following live vaccination of parent chickens by two different routes.vaccination of broiler breeder parents with live infectious bursal disease (ibd) vaccine by the oral route resulted in higher and more persistent quantitative gel diffusion precipitins than vaccination by intramuscular injection. all the progeny of the orally vaccinated parents had maternally derived ibd antibody (mda) at hatching while mda was detected in only 35 per cent of the progeny of the intramuscularly vaccinated parents. this mda had disappeared completely at 14 and eight days respectiv ...19806249014
immunofluorescent detection of double-stranded rna in cells infected with reovirus, infectious pancreatic necrosis virus, and infectious bursal disease virus.urea treatment of ethanol-fixed virus-infected cells exposed nucleic acid antigens for immunofluorescence. three double-stranded (ds) rna-containing viruses showed bright fluorescence using antibodies against dsrna. three single-stranded rna-containing viruses showed less intense fluorescence with anti-dsrna. four out of five cell lines persistently infected with various rna-containing viruses showed no dsrna detectable by immunofluorescence.19806250691
ubiquity of infectious bursal disease in east texas backyard flocks.sera from 25 "backyard" flocks were screened by agar-gel precipitin (agp) and virus-neutralization (vn) tests for antibodies to infectious bursal disease virus. although titers varied from flock to flock, 100% of the birds screened had virus-neutralizing antibodies. in general, birds with high vn titers had positive agp reaction.19806251442
pathology of nephrosis-nephritis in chickens experimentally induced by infectious bursal disease virus. 19806253391
performance of 3 successive generations of specified-pathogenfree chickens maintained as a closed flock.no antibodies against salmonella pullorum, mycoplasma gallisepticum, mycoplasma synoviae, haemophilus gallinarum, fowl pox virus, marek's disease virus, herpes virus of turkey, infectious laryngotracheitis virus, avian adenovirus, avian reovirus, infectious bursal disease virus, reticuloendotheliosis virus, avian leukosis virus, avian encephalomyelitis virus and newcastle disease virus were detectable in the sera obtained from these chickens in 3 generations at various ages. antibodies against i ...19806253742
the natural prevalence of antibodies to infectious bursal disease in australian poultry flocks. 19806254480
response of mibolerone-treated chickens to infectious bursal disease virus.the response of mibolerone-treated chickens to infectious bursal disease virus (ibdv) was studied. chickens fed a ration containing mibolerone at 1.5 parts per million (ppm) developed bursal atrophy by 4 weeks of age. no indication was found that mibolerone prevented ibdv infection even after 7 weeks of feeding. furthermore, only chickens treated with mibolerone died when infected with ibdv at 2 or 3 weeks of age, and treated chickens had more severe clinical signs than did untreated controls wh ...19806254486
an indirect enzyme-linked immunosorbent assay (elisa) for measuring antibodies in chickens infected with infectious bursal disease virus.an immuno-enzyme assay for measuring infectious bursal disease antibodies in chickens is described. the test is performed rapidly after coating plates overnight with partially purified antigen prepared in cell culture. coated plates can be stored for at last 4 months. the chromatographically purified rabbit anti-chicken immunoglobulin-g, conjugated to horseradish peroxidase, was used optimally at a dilution of 1:3,000. it could be stored for at least 10 months without a reduction in titer. the t ...19806254489
field studies with convalescent serum and infectious bursal disease vaccine to control turkey coryza.convalescent serum given to 1-day-old poults delayed clinical signs of turkey coryza by several days and reduced mortality on infected farms. turkey breeders immunized with cell-culture-adapted infectious bursal disease virus (ibdv) or turkey infectious bursal disease virus (tibdv) had a marked increase in virus-neutralization (vn) antibody titers. the vn antibody titer was significantly higher in progeny poults than in poults from unimmunized breeders. clinical turkey coryza and mortality was c ...19806254490
infectious bursal disease virus: further characterization with evidence for a single-stranded rna virus.infectious bursal disease virus (ibdv) that had been adapted to grow and was then cloned in chick embryo fibroblast (cef) cell culture was examined for its physicochemical properties, the cellular site of virus replication, and the nature of its viral rna. the ibdv was an rna virus, acid-stable, absolutely resistant to chloroform, and moderately thermolabile. it appeared to replicate only in the cytoplasm, as shown by virus-specific antigens restricted to the cytoplasm of infected cells. the vir ...19806254492
computer simulation of gumboro disease outbreak. iii. construction model g-4.following the simulation mode., g-3, of gumboro disease outbreak, model g-4 was constructed. the algorithm for computer simulation is shown in a flow chart. the postulates added to those for models g-1 and g-2 are as follows: (1) the source of contamination is the virus remaining in the house and declining gradually in value with the lapse of time. (2) any diseased bird excretes the virus during a certain period, so that the virus may be added to the source of contamination. (3) the morbid statu ...19806255346
computer simulation of gumboro disease outbreak. iv. epizootics obtained by mode g-4 with flock size, age and immunity changed.for the computer simulation of gumboro disease outbreak by model g-4 a program was written in fortran. of over twenty parameters involved in the model, the following three were used as input variables: (1) age of chickens at housing a1 (= 1 and 21); (2) size of flock at housing, n(a1) (= 100 and 1,000); and (3) geometric mean of the level of parentally conferred immunity at hatching, g(1) (= 0, 2, 8, and 32). the outputs were the graphic images demonstrating the chronological changes in a flock ...19806255347
isolation of infectious bronchitis, infectious bursal disease and avian adenoviruses from chickens with nephritis-nephrosis syndrome. 19806255706
an age-related coagulation disorder associated with experimental infection with infectious bursal disease virus.specific-pathogen-free white leghorn chickens were inoculated with a field strain of infectious bursal disease virus. one group (a) was inoculated at 17 days after the chicks were hatched, and the other groups (c and e) were inoculated at posthatch day 42. blood samples were obtained for determination of clotting times (whole blood recalcification, prothrombin, and activated partial thromboplastin times), virus-neutralizing antibody, and total hemolytic complement. there were significant increas ...19806255838
immune profile of infectious bursal disease: i. effect of infectious bursal disease virus on peripheral blood t and b lymphocytes of chickens.a comparison was made of absolute numbers of peripheral blood b and t lymphocytes, as defined by the anti-bursal and anti-thymus sera, in chickens infected with infectious bursal disease virus at one day and 3 weeks of age by age-matched controls. birds were evaluated sequentially at weekly intervals for a period of 8 weeks postinfection. the severity of depressions of b-lymphocyte numbers was found to be age-dependent: the birds infected at one day of age showed a more severe depletion than tho ...19806255926
immune profile of infectious bursal disease (ibd). ii. effect of ibd virus on pokeweed-mitogen-stimulated peripheral blood lymphocytes of chickens.pokeweed-mitogen-stimulated peripheral blood lymphocytes from chickens infected with infectious bursal disease virus (ibdv) at 1 day and 3 weeks of age, together with those from uninfected age-matched control chickens, were examined at weekly intervals for their capacity to undergo in vitro terminal differentiation. this study included the determination of cytoplasmic-immunoglobulin (cig)-containing peripheral blood lymphocytes and those bearing the surface membrane immunoglobulin (smig) cells t ...19806255927
antibodies to infectious bursal disease virus in poultry flocks in western australia. 19806257220
[isolation of cytopathogenic strains of infectious bursal virus of chickens in cell cultures].five virus strains with cytopathogenic properties for the cell cultures of chick embryo fibroblasts and embryo kidney cells were isolated from chickens clinically suspected of suffering from infectious bursitis. according to the form of cytopathic effect (cpe) on cell cultures, according to chloroform and thermal resistance, nature of nucleic acid (rna), according to the absence of the production of intracellular inclusions, negative haemagglutination, and according to antigenic identity with th ...19806258280
booster effect of revaccination against newcastle disease on chickens infected with infectious bursal disease virus. 19806262549
the detrimental effect of vaccinating parentally immune broilers with a modified liver virus vaccine for infectious bursal disease.the experiment involved 392,955 broilers from breeders vaccinated with a commercially available killed infectious bursal disease (kibd) virus vaccine (treatments 1 and 2) and 373,371 broilers from breeders not vaccinated with a kibd virus vaccine (treatment 2). the broilers from the kibd-vaccinated breeders were divided into two treatment groups: treatment 1 consisted of 209,457 broilers and treatment 2 consisted of 183,498 broilers. treatment 2 broilers were vaccinated at 10 days of age intraoc ...19806268038
effects of infectious bursal disease on marek's disease vaccination: suppression of antiviral immune response.studies were made to determine whether infectious bursal disease virus (ibdv) infection would affect the response of chickens to turkey herpesvirus (hvt) vaccination in the development and level of hvt viremia and virus-neutralizing (vn) antibodies to hvt. the hvt viremia in the vaccinated chickens was not affected by ibdv, whether ibdv was inoculated simultaneously with hvt vaccination at one day of age or whether it was inoculated 3 weeks postvaccination with hvt. however, vn antibody response ...19806268042
comparison of inactivated and live infectious bursal disease virus vaccines in white leghorn breeder flock.leghorn breeder chickens, which had received live infectious bursal disease (ibdv) virus vaccine at 12 weeks of age and had been injected subcutaneously at 20 weeks of age with an inactivated ibdv oil emulsion vaccine, produced very high antibody titers which persisted for at least 9 months and were more uniform than those obtained from breeders receiving only the live ibdv vaccine. breeders vaccinated with the inactivated ibdv oil emulsion vaccine at 12 and 20 weeks of age had antibody titers h ...19806267575
observations on alcaligenes faecalis infection in turkeys.experiments were initiated to study the pathogenicity of 5 alcaligenes faecalis isolates in specific-pathogen-free poults. the isolates were recovered from commercial flocks suffering from a respiratory disease. there were no differences between cultural or biochemical characteristics of the isolates, but differences in antibiotic sensitivity were detected. all 5 isolates were capable of initiating a respiratory disease in poults similar to that seen in the early stages of turkey coryza. the inf ...19806449924
infectious bursal disease virus. 19807000445
the distribution of naturally acquired antibodies against infectious bursal disease virus in victoria. 19816268036
immune profile of infectious bursal disease. iii. effect of infectious bursal disease virus on the lymphocyte responses to phytomitogens and on mixed lymphocyte reaction of chickens.a whole-blood-culture technique was used to sequentially evaluated peripheral blood lymphocyte responses to phytohemagglutinin (pha) and concanavalin a (con a) of normal chickens and chickens infected at 1 day or 3 weeks of age with infectious bursal disease virus (ibdv). this method had numerous advantages over the more conventional techniques. a comparative study was made on the percentage of inhibition of responses of peripheral blood lymphocytes to pha and con a of 1-day- and 3-week-old ibdv ...19816268047
improved performance of progeny of broiler parent chickens vaccinated with infectious bursal disease oil-emulsion vaccine.field trials were conducted on nine broiler chicken flocks, which were the progeny of parents vaccinated with inactivated infectious bursal disease (ibd) oil-emulsion vaccine (oev). the average increase in overall weight gains was 7.97% of weight gains of similar birds from live-vaccinated parents. food-conversion ratios (fcr) were calculated for five of the trials. the average improvement in fcr over that of the control chickens was 2.89%. every broiler flock contracted subclinical ibd, but the ...19816268051
subclinical infectious bursal disease in commercial broiler flocks in saskatchewan.five commercial broiler flocks, not vaccinated for infectious bursal disease virus, derived from infectious bursal disease virus-vaccinated breeder flocks were surveyed for evidence of bursal damage and infectious bursal disease virus infection. they were compared with two groups of birds raised in isolation. serum samples from one day old chicks contained maternal anti-infectious bursal disease virus antibodies which declined to undetectable levels by four weeks of age. serum antibody levels re ...19816268263
an enzyme-linked immunosorbent assay (elisa) for infectious bursal disease virus.the application of the indirect enzyme-linked immunosorbent assay (elisa) for detection of infectious bursal disease virus antibodies in chicken serum was investigated. the test procedure involved the coating of concentrated infectious bursal disease virus antigen onto polystyrene tubes, followed by the addition of chicken anti-infectious bursal disease virus serum and horseradish peroxidase labeled rabbit anti-chicken globulin. as an indicator substrate, 5-aminosalicylic acid, with the oxidant ...19816268265
plasma cell changes in the gland of harder following infectious bursal disease virus infection of the chicken.tissue changes in the gland of harder (gh) were studied following infection of day-old chicks with infectious bursal disease virus (ibdv). broiler chicks that lacked maternal antibody to ibdv were inoculated with ibdv at 1 day of age and were maintained segregated from uninoculated controls. plasma cell (pc) content of the gh was lowered among infected chickens from 1 to 7 weeks postinoculation. since the gh in uninoculated control birds was not densely populated with pcs until 3 weeks of age, t ...19816274296
an outbreak of infectious bursal disease among chickens between 16 and 20 weeks old.infectious bursal disease (ibd) was diagnosed in a flock of 1,031 broilers and cockerels aged between 16 and 20 weeks. affected birds passed whitish, watery feces. on postmortem examination, the bursa of fabricius (bursa) was enlarged and the kidney tubules were well distended. histopathological sections of the bursa were characterized by edema, destruction of lymphocytes, and heterophilic infiltration. spread was rapid, and the average mortality rate was 3.5%. bursal homogenates from dead chick ...19816279063
comparison of precipitin antibodies and virus-neutralizing antibodies to infectious bursal disease virus.three hundred twenty-two serum samples from commercial pullets and multiplier breeders were analyzed for agar-gel precipitin (agp) antibodies and virus-neutralizing (vn) antibodies to infectious bursal disease virus. two hundred thirty-four of these sera were agp-positive, and 88 were agp-negative. the geometric mean of the reciprocal of the vn titers for the agp-positive sera was 208.7, and 232 (99.1%) had a vn titer of 1:16 or greater. in contrast, the geometric mean of the reciprocal of the v ...19816279066
in vitro infection of fractionated chicken lymphocytes by infectious bursal disease virus.lymphocytes from bursa of fabricius and thymus of chickens were purified and separated into the three cell subsets--t, b, and null cells--by the techniques of nylon fiber columns and cytotoxicity tests. the in vitro susceptibility of the fractionated lymphocytes to a virulent strain of infectious bursal disease virus (ibdv) was studied by using immunofluorescence as the infection criterion. b cells were highly susceptible. by contrast, t cells and null cells were insusceptible to infection by ib ...19816279071
characteristics of apparent derivatives of the 2512 strain of infectious bursal disease virus when used as vaccines.the 2512 infectious bursal disease virus (ibdv) strain maintained in the authors' laboratory was compared with apparent 2512 ibdv isolates designated i-2512, p-2512, and h-2512. the latter three viruses were obtained from different sources and, ostensibly, had their origin in the purdue laboratory. their effects on immunogenicity, transmissibility, pathogenicity, and cell cultures varied. one of these isolates was said to be only 2 embryo passages higher than the original seed virus in our labor ...19816279076
growth and infectivity titration of virulent infectious bursal disease virus in established cell lines from lymphoid leukosis tumors.lymphoblastoid cell lines were investigated for susceptibility to virulent (wild) infectious bursal disease virus (ibdv) and attenuated ibdv by detection of fluorescent antigen in inoculated cells. lscc-bk3 and lscc-cu10 cells were susceptible to both types of ibdv; lscc-1104-b-1 line was susceptible to only the attenuated ibdv. however, 2 lymphoid leukosis and 2 marek's disease cell lines were refractory to growth of any type of ibdv. when the j1 strain of virulent ibdv was inoculated into lscc ...19816279078
pathogenesis of infectious bursal disease in embryonally bursectomized chickens.the pathogenesis of infectious bursal disease (ibd) in intact chickens was compared with pathogenesis in chickens that had undergone embryonal bursectomy (ebx chickens), which were challenged at either 2 or 6 weeks of age. all ebx chickens were free of bursa remnants, and those challenged at 6 weeks of age failed to develop primary and secondary antibody responses to sheep red blood cells and bovine serum albumin. a direct fluorescent-antibody technique was used to study the course of infection ...19816279079
sequential mitogen stimulation of peripheral blood lymphocytes from chickens inoculated with infectious bursal disease virus.chickens were inoculated wih the pathogenic edgar strain of infectious bursal disease virus at 1 week, 2 weeks, or 1 day of age. in the 3 experiments, phytohemagglutinin stimulation of peripheral blood lymphocytes was significantly decreased on day 3 or 4 after inoculation. subsequently, on days 7 through 21, stimulations were similar between lymphocytes from inoculated birds and those from control birds. pokeweed mitogen stimulation was affected minimally in virus-inoculated chickens. in each e ...19816280521
identification of a strain of infectious bursal disease virus isolated from mosquitoes.the becht strain of infectious bursal disease virus was compared with a virus isolated from aedes vexans mosquitoes and designed 743 virus. the viruses were compared with respect to cell culture host range, cellular changes resulting from viral infections, growth curves, antigenic relationship, and physicochemical characteristics. the viruses are closely comparable in all these properties, and they are considered to be strains of the same virus. in cross comparisons by the enzyme-linked immunoso ...19816280817
persistence of infectious bursal disease virus in experimentally infected chickens. 19816282243
immune responses of breeding chickens to trivalent oil emulsion vaccines: responses to newcastle disease and infectious bursal disease.bivalent newcastle disease (nd)/infectious bursal disease (ibd) and trivalent nd/ibd/infectious bronchitis (ib) inactivated oil emulsion vaccines were prepared in the laboratory and evaluated under field conditions. broiler breeder parent chickens previously vaccinated with live vaccines were inoculated with commercial monovalent nd and experimental bivalent or trivalent oil emulsion vaccines. the commercial vaccine induced a higher initial nd haemagglutination inhibition (hi) response than the ...19816266123
immune responses of breeding chickens to trivalent oil emulsion vaccines: responses to infectious bronchitis.three similar flocks of broiler breeder parent chickens that had been given live infections bronchitis (ib) vaccines during rearing were injected at 20 weeks of age with three different oil emulsion vaccines: a commercial monovalent newcastle disease (nd) vaccine (flock a); an experimental bivalent vaccine containing nd and infectious bursal disease (ibd) components (flock b); and an experimental trivalent vaccine containing nd, ibd and ib components (flock c). one week after vaccination 40 hens ...19816266125
effect of infectious bursal disease virus infection on the phagocytosis of staphylococcus aureus by mononuclear phagocytic cells of susceptible and resistant strains of chickens.studies were conducted to determine why chickens infected with infectious bursal disease (ibd) are more susceptible to staphylococcus aureus. we evaluated the effect of ibd virus on functions of the peripheral mononuclear phagocytes and looked for humoral abnormalities in the serum. an in vitro opsonophagocytic assay using (h3) thymidine-labeled opsonized staphylococcus aureus was used to measure phagocytosis. infection of ibd-sensitive (scwl 420) chickens with ibd virus did not cause any cellul ...19816266387
sequential changes in the number of surface immunoglobulin-bearing b lymphocytes in infectious bursal disease virus-infected chickens.variations of b lymphocytes bearing surface immunoglobulin (sig) m [sig(m)] and g [sig(g)] were studied in the spleen and peripheral blood of chickens infected with infectious bursal disease virus (ibdv). the proportion of sig-bearing b lymphocytes and sig(m)- and sig(g)-bearing b cells in chickens infected at one day of age decreased from 1 week postinfection (p.i.) onward and was significantly lower at 8 weeks p.i. in chickens infected at 4 weeks, the percentage of sig-bearing b cells decrease ...19816266392
[laboratory diagnosis of infectious bursal disease (ibd) in northern nigeria (1975-1979)]. 19816286016
influence of hormonal and chemical bursectomy on the development of acquired immunity to coccidia in broiler chickens.the effect of bursectomy on the development of acquired immunity to coccidiosis in young broiler chickens was examined. bursectomy was produced by a combination injection of testosterone at 12 days of embryonation and cyclophosphamide at 1 and 2 days after hatching. immunity to coccidiosis developed in bursectomized chickens immunized with commercially prepared vaccine (coccivac d) as were measured by resistance to challenge infection at either 6 or 10 weeks of age. bursectomy had no marked effe ...19816283508
bacterial coryza in turkeys in texas.a motile, gram-negative, short bacillus was isolated from the tracheas of turkey poults with coryza. an escherichia coli also was isolated from the tracheas of poults. the former bacterium possessed characteristics similar or identical to those isolated from coryza outbreaks in other states. the characteristics were similar to those described for alcaligenes fecalis. cultures of the turkey coryza isolate produced coryza when inoculated intranasally in 1 to 3-day-old poults. the bacterium was rei ...19817232255
the interaction of live vaccine and maternal antibody in protection against infectious bursal disease.an experiment was performed to determine the results of the interaction of a mild infectious bursal disease vaccine and two different levels of maternal antibody in chicks, at various ages after hatching, as judged by protection against challenge with virulent infectious bursal disease virus. maternal antibody of both high and low initial levels prevented effective vaccination at 1 and 14 days of age but by 28 days of age the vaccine was effective in birds of both initial antibody levels. only t ...198118770150
rapid detection of infectious bursal disease antibodies by counterimmunoelectrophoresis.counterimmunoelectrophoresis was used to detect infectious bursal disease virus-specific precipitating antibodies in chicken sera. the method was found to be sensitive, simple, reproducible and rapid - detecting precipitins after 45 min. results could be read visually, without staining, and they compared favourably with results obtained with the agar-gel precipitation test.198218770227
serological investigations of infectious bursal disease virus and reticuloendotheliosis virus infections in new zealand chickens. 198216030899
duration of immunosuppression caused by a vaccine strain of infectious bursal disease virus.the depression of response of brucella abortus strain 19 caused by an infectious bursal disease vaccine virus given to chicks at one day old was shown to persist for four weeks. histological examination of the bursa of fabricius showed a gradual repopulation by bursal lymphocytes, after initial damage, concomitant with the development of a humoral response.19826283614
comparison of the efficacy of four inactivated infectious bursal disease oil emulsion vaccines.a comparison was made between four inactivated infectious bursal disease (ibd) oil emulsion vaccines. vaccine a, which was prepared from antigen derived from bursae of fabricius, stimulated higher levels of ibd gel diffusion titres than vaccines b, c and d, which were prepared from antigens derived from embryo fluids. the progeny of parents vaccinated with vaccine a resisted ibd challenge for eight days longer than the progeny of any of the other vaccinated parents.19826283716
the immune response of the chick following viral vaccinations and immunization with sheep red blood cells.the effects of viral vaccinations and immunization with sheep red blood cells (srbc) on the humoral response of pullets were investigated. pullets were vaccinated with marek's disease virus, newcastle disease virus (ndv), infectious bronchitis virus (ibv), and infectious bursal disease virus at appropriate ages used in commercial practice. at seven weeks, the pullets were intramuscularly immunized with srbc. ndv and ibv antibodies were detected by hemagglutination-inhibition tests. hemagglutinat ...19826284114
correlation of hematological changes and serum and monocyte inhibition with the early suppression of phytohemagglutinin stimulation of lymphocytes in experimental infectious bursal disease.several experiments were conducted to study the mechanism of infectious bursal disease virus induced suppression of phytohemagglutinin stimulation of peripheral blood lymphocytes. infectious bursal disease virus inoculation of one week old chicks resulted in significant suppression of phytohemagglutinin stimulation during the first three days after inoculation as demonstrated by a whole blood assay. mild thymic necrosis was seen on day 3. hematological changes during this time consisted of incre ...19826284327
infectious bursal disease virus and alcaligenes faecalis infections in turkeys.to determine if infectious bursal disease virus (ibdv) augments alcaligenes rhinotracheitis (art), turkey poults were exposed to ibdv, alcaligenes faecalis, or both ibdv and a. faecalis. in five experiments, poults exposed to ibdv alone exhibited neither signs of disease nor histopathologic lesions. serum antibodies to ibdv were detected in poults exposed to this virus by inoculation and by direct contact with inoculated birds. signs of art were observed 4 to 6 days following exposure to a. faec ...19826285882
serologic evidence of infectious bursal disease virus infection in iowa turkeys.two infectious bursal disease viruses (ibdvs)--a cell-culture-adapted chicken strain designated edgar strain and a recent isolate from turkeys in missouri--were used to assay sera from 10 iowa turkey flocks collected between 1 and 16 weeks of age. the two viruses were serologically distinct in cross-neutralization tests. for all flocks, a similar serologic pattern was found consisting of (1) low maternal antibody titers to turkey ibdv and occasionally to edgar strain ibdv between 1 and 3 weeks o ...19826184043
recovery of virus from feces and tissues of chickens infected with cell-culture-adapted infectious bursal disease virus. 19826287071
increased severity and new symptoms of infectious bursal disease during aflatoxicosis in broiler chickens.a factorial design for the presence and absence of infection with a mild strain of infectious bursal disease (ibd) virus and graded levels of dietary aflatoxin (0, .625, 1.25, 2.5, 5.0, and 10.0 microgram/g of diet) in young broiler chickens revealed several interactions which greatly changed and character of the ibd infection. an infection that was essentially free of mortality became lethal to about half the birds. a coagulopathy as indicated by prolonged prothrombin times and by the occurrenc ...19826287447
factors affecting the reproducibility of the serum neutralization test for infectious bursal disease. 19826290498
field trials with an oil emulsion infectious bursal disease vaccine in broiler breeder pullets.two independent field trials were undertaken with commercial broiler companies in separate sections of alabama in order to test the efficacy of an experimental commercially prepared oil emulsion infectious bursal disease (ibd) vaccine in broiler breeder pullets. the ibd vaccine was tested both for safety and the production of neutralizing antibodies in pullets and their progeny as well as its ability to prevent early field exposure to virulent ibd virus (ibdv) in broiler progeny that may lead to ...19826291020
biosynthesis of virus-specific proteins in cells infected with infectious bursal disease virus and their significance as structural elements for infectious virus and incomplete particles.it has previously been shown that infectious bursal disease virus is a naked icosahedral particle with a diameter of about 60 nm and a genome consisting of two segments of double-stranded rna (müller et al., j. virol. 31:584-589, 1979). one of the two major structural polypeptides (molecular weight, 40,000) of this virus could not be found in lysates of infected cells; it is derived from a precursor polypeptide demonstrable inside the cells in relatively large quantities and seems to be processe ...19826292499
efficacy of some disinfectants against infectious bursal disease virus and avian reovirus. 19826293154
studies on a vaccine against infections bursal disease.an infectious bursal disease vaccine, registered for use in breeder flocks, was studied for efficacy on the day-old offspring of vaccinated hens and for virulence in susceptible day-old and 6-week-old chickens. when given to susceptible day-old chicks and 6-week-old cockerels, the vaccine was found to induce atrophy and pathology of the bursa of fabricius similar to that observed in field infections. chicks vaccinated at day-old had markedly lowered titres in the haemagglutination inhibition tes ...19826293438
lesions induced in the respiratory tract of chickens by serologically different adenoviruses.ten strains of adenovirus representing 10 serotypes were administered intratracheally to two groups of 3-week-old specific-pathogen-free chickens. birds in group 1 were given only adenovirus. birds in group 2 were inoculated with a virulent infectious bursal disease virus (ibdv) by eye-drop at one day of age as well as with the adenovirus at 3 weeks. the chicks were examined daily. respiratory rales were observed in some birds with the dual infection. gross pathologic alterations were minimal an ...19826293441
susceptibility of ducks and duck-origin cell cultures to infectious bursal disease virus.specific-pathogen-free (spf) ducks that were 1, 3, 4, 7, 10, 30, and 180 days old were inoculated experimentally orally or nasally with infectious bursal disease virus (ibdv). attempts to induce clinical disease in ducks with strain j1 or fk-78 of ibdv were unsuccessful. virus-recovery attempts from organ and intestinal contents were also unsuccessful. no significant gross or histopathological lesions were found in liver, spleen, kidney, heart, or bursa of fabricius of 1- and 3-day-old ducks at ...19826293447
humoral and cell-mediated immune responses in chickens with infectious bursal disease.primary and secondary immune responses to newcastle disease virus (ndv) was evaluated in chickens infected with infectious bursal disease virus (ibdv) at one and 28 days of age. the geometric mean primary hemagglutination-inhibition antibody titers (gmt) of chickens infected with ibdv at one day of age was significantly lower (p less than or equal to 0.01) than those infected at 28 days of age. infection with ibdv had no influence on secondary immune response to ndv. the effect of ibdv infection ...19826294964
field test studies of the 2512 strain of infectious bursal disease.responses to an infectious bursal disease vaccine made from the 2512 strain were studied in broiler flocks under field conditions and management. pre- and post-vaccination sera were analyzed by agar-gel-precipitin tests and beta virus neutralization. specific measurements of broiler performance (livability, weight, feed conversion, and condemnations) were also analyzed. when possible, contemporary licensed products were compared at the same time in adjacent houses. results of these trials indica ...19826297449
characteristics and serologic studies of two serotypes of infectious bursal disease virus in turkeys.two serotypes of infectious bursal disease virus (ibdv) were distinguished using the virus-neutralization test. an ibdv isolate from chickens, two commercial ibdv vaccine viruses, and an ibdv isolate from turkeys were designated serotype i. two ibdv isolates from turkeys were designated serotype ii. a common antigen shared by serotype i and serotype ii viruses was detected using an indirect immunofluorescent assay. the virions of both ibdv serotypes were determined to be nonenveloped icosahedron ...19826297451
[immunoprophylaxis of infectious bursitis in poultry]. 19826304990
the bursa: fabricius to delaware. 19826283078
survey of some poultry viruses in papua new guinea.during the years 1974 to 1979 a virological study on domestic poultry throughout papua new guinea was conducted involving clinical examination of diseased birds with subsequent attempted virus isolations and serological surveys of free village fowls and commercial poultry. viruses isolated included those of newcastle disease, infectious bronchitis, pox, avian encephalomyelitis and adenovirus. clinical and pathological diagnoses of pox, avian encephalomyelitis, reticuloendotheliosis and marek's d ...19827101463
a rapid quantitative agar gel precipitin test. 19836306011
infectious bursal disease in western australia. 19836307244
use of inactivated oil emulsion infectious bursal disease vaccines in breeder chickens to prevent immunosuppression in progeny chicks.two chicken flocks, vaccinated with different inactivated infectious bursal disease vaccines, and one unvaccinated flock provided chicks with high and low levels of and no maternally derived immunity. following challenge at three ages with a subclinical strain of infectious bursal disease virus the chicks were assessed for bursal damage and suppression of the immune response to newcastle disease virus. both high and low levels of maternally derived antibody prevented immunosuppression but the lo ...19836312519
effects of dual infection of chickens with mycoplasma synoviae and mycoplasma gallinaceum or infectious bursal disease virus on infectious synovitis. 19836314027
multivalent inactivated virus oil emulsion vaccines in broiler breeder chickens. i. newcastle disease virus and infectious bursal disease virus bivalent vaccines.inactivated newcastle disease virus (ndv) and infectious bursal disease virus (ibdv) were incorporated into water-in-oil emulsion vaccines alone or as a bivalent vaccine. twenty-week-old broiler breeder chickens that had received previous live virus vaccination with ndv and ibdv were injected intramuscularly with the monovalent or bivalent vaccine. the antibody titers to either the monovalent vaccine or bivalent vaccine increased rapidly and then remained at high levels for the duration of the 4 ...19836314307
multivalent inactivated virus oil emulsion vaccines in broiler breeder chickens. ii. trivalent vaccines in breeders not previously vaccinated with live newcastle disease, infectious bursal disease, and tenosynovitis vaccines.inactivated newcastle disease (ndv), infectious bursal disease (ibdv), and viral arthritis/tenosynovitis (va) viruses were incorporated into water-in-oil emulsion vaccines either alone, in bivalent combinations, or in a trivalent vaccine. twenty-week-old broiler breeder chickens with no previous exposure to ndv, ibdv, or va live virus vaccines were injected intramuscularly with the monovalent, bivalent, or trivalent vaccines. the antibody responses to ndv in all three vaccines were poor, and ndv ...19836314308
multivalent inactivated virus oil emulsion vaccines in broiler breeder chickens. iii. trivalent newcastle disease, infectious bursal disease, and arthritis/tenosynovitis viruses vaccine in primed breeders.inactivated newcastle disease (ndv), infectious bursal disease (ibdv), and viral arthritis/tenosynovitis (va) viruses were incorporated into water-in-oil emulsion vaccines, either alone, in bivalent combinations, or in a trivalent vaccine. twenty-week-old broiler breeder chickens that had received previous live virus vaccination with ndv, ibdv, and va were injected intramuscularly with the monovalent, bivalent, or trivalent vaccines. the antibody titers to the antigens contained in the monovalen ...19836314309
maternal antibody and its effect on infectious bursal disease immunization.chickens vaccinated with infectious bursal disease virus (ibdv) early in life and revaccinated with an inactivated, oil-adjuvant ibdv vaccine at 18 weeks of age produced and maintained high levels of virus-neutralizing (vn) antibody through 10 months of lay. vn-antibody titers of chicks hatched from eggs laid during the same period closely matched the average vn-antibody titers of the dams. a sequential study of the decline rates of ibdv maternal antibody (mab) in unvaccinated and ibdv-vaccinate ...19836314972
pathogenesis of infectious bursal disease in chickens infected with virus at various ages.chickens free from infectious bursal disease (ibd) maternal antibody were inoculated with a virulent strain of ibd virus at 1, 5, or 11 weeks of age. chickens inoculated at 5 weeks developed severe clinical signs and had reduced levels of serum complement within 2-4 days postinoculation, but those inoculated at 1 or 11 weeks did not. however, at 1, 2, 4, and 8 days postinoculation, the rate of virus recovery from different tissues, severity of microscopic lesions, and frequency of detection of v ...19836314977
prevalence of antibodies to infectious bursal disease virus serotypes i and ii in 75 ohio chicken flocks.serum samples from 75 ohio layer flocks were tested for antibodies to infectious bursal disease virus (ibdv) serotypes i and ii using a virus-neutralization test. infectious bursal disease had been diagnosed in three of the 75 flocks. antibody titers to serotype i were detected in 77% of the flocks, and antibody titers to serotype ii were detected in 47% of the flocks. of the eight flocks known to have been vaccinated using a serotype i ibdv vaccine, all had antibody titers to serotype i, and fo ...19836314983
effect of infectious bursal disease on natural killer cell activity and mitogenic response of chicken lymphoid cells: role of adherent cells in cellular immune suppression.a pathogenic isolate of infectious bursal disease virus (ibdv) caused persistent and extensive lesions in the bursa but mild and transient lesions in the thymuses of chickens of lines 63 and p. the effect of ibdv on two cellular immune functions, namely, natural killer cell cytotoxicity and mitogenic response, was studied. the natural killer cell activity was not consistently influenced, but the virus, during the first 2 weeks of infection, caused transient depression of the blastogenic response ...19836315586
infectious bursal disease in the sudan. 19836316595
newcastle disease in a vaccinated flock which had experienced subclinical infectious bursal disease.a flock of 4,500 cobb broilers inoculated with newcastle disease vaccine intra-ocular strain b1 type at 10 days of age developed clinical signs of the disease 19 days later; the mortality rate was 71%. necropsy examinations showed characteristic lesions. newcastle disease virus was isolated and identified in the allantoic fluid of embryonating chicken eggs by haemagglutination and haemagglutination-inhibition tests. histopathological examination showed that follicles of the bursa were depleted o ...19836316596
the pathogenesis of infectious bursal disease: serologic, histopathologic, and clinical chemical observations.infectious bursal disease in 35-day-old specific-pathogen-free (spf) chickens was characterized clinically by its acute onset and brief duration. clinical signs included depression, anorexia, diarrhea, and polyuria. a detectable precipitin antibody response occurred between 3 and 5 days postinoculation. evaluation of pooled serum samples obtained from infectious bursal disease virus (ibdv)-infected chickens revealed transient changes in potassium, cholesterol, uric acid, lactate dehydrogenase, s ...19836316894
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