Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| distribution and titres of rotavirus antibodies in different age groups. | three hundred and fifty-seven sera selected at random from hospital patients of all ages were examined for rotavirus antibodies using indirect immunofluorescence (fa) and complement fixation levels (cft). three hundred and fourteen of these were also tested for neutralizing antibodies to human rotavirus. sera from patients admitted with a diagnosis of acute gastroenteritis were excluded from this survey. fa antibodies were found in newborn infants but fell to undetectable titres at 3 months. the ... | 1977 | 200676 |
| observations on the morphology of two rotaviruses. | the negative staining technique was used to study the morphology of two rotaviruses, the epizootic diarrizootic diarrhoea of infant mice (edim) and the simian virus sa ii. it is proposed that the inner capsid of the virion has icosahedral symmetry and consists of 180 morphological units arranged in an open lattice formation with the 12 spaces at the apices being surrounded by 5 capsomeres and the other 80 spaces each surrounded by six capsomeres. the outer capsid of the virion consists of a hone ... | 1977 | 200716 |
| cell culture propagation of porcine rotavirus (reovirus-like agent). | two isolates of porcine rotavirus (reovirus-like agent) were isolated and passaged in primary procine kidney cell cultures. viral infectivity for cells was monitored by immunofluorescence because viral cytopathic effect was moderate. successful passage of virus in cell culture required that viral suspensions obtained from infected cell cultures be treated with pancreatin prior to inoculation onto cell monolayers. porcine rotavirus passage in cell culture also was accomplished, using trypsin trea ... | 1977 | 201198 |
| rotavirus neutralisation by human milk. | 1977 | 201336 | |
| an antigenic subunit present in rotavirus infected faeces. | it has been found by immune electron microscopy that rotavirus-infected faeces, calf or human, contain an antigenic subunit associated with the inner of the two virus capsids. this internal component represents the group specific antigen for the rotavirus group and the subunit reacts with both homologous and heterologous antiserum. it can therefore be used in diagnostic tests and in this paper its use as a reagent for immunodiffusion is described. | 1977 | 65444 |
| antigenic relationship between human and simian rotaviruses. | the simian rotavirus, sa 11, and the murine rotavirus, edim, were investigated for antigenic relatedness to the human rotavirus, by immunoelectron-microscopy. these studies led to the recognition of two types of rotavirus antibody. one agglutinated "rough" virus particles only and was group-reactive; it appears to be widely distributed in various animal species, including human infants. the second antibody agglutinated "smooth" virus particles and was more species-specific, demonstrating only a ... | 1977 | 65473 |
| identification of rotaviruses. | 1977 | 65702 | |
| rotavirus antibodies in crohn's disease and ulcerative colitis. | 1977 | 68365 | |
| serological relationships between rotaviruses from different species as studied by complement fixation and neutralization. | human, piglet, mouse, foal, lamb, calf and rabbit rotaviruses all infected, but could not readily be subcultured in llc mk2 cells. cells infected with mouse and calf rotaviruses reacted by indirect immunofluorescence (fa) with convalescent serum from children, piglets, mice, foals, lambs, calves or rabbits, taken after rotavirus infection. human, calf, piglet, mouse and foal rotaviruses reacted with human, calf, mouse, foal and lamb convalescent serum by complement fixation (cf). it was not poss ... | 1977 | 68765 |
| detection of infantile gastroenteritis virus (rotavirus) by elisa. | 1977 | 69078 | |
| cell culture studies with a cytopathic bovine rotavirus. | the growth characteristics in mdbk cells of a calf rotavirus isolated in northern ireland are described. of a range of cell cultures tested, the virus grew in secondary bovine kidney and mdbk cells, but consistently produced a cpe only in mdbk cells. the cpe consisted of cytoplasmic vacuolation, development of eosinophilic intracytoplasmic inclusions, and degeneration and detachment of cells from the monolayer. the onset of cpe was more rapid and its effects more severe in rolled cultures than s ... | 1977 | 70198 |
| elisa for rotavirus. | 1977 | 71624 | |
| rotavirus infections of neonates. | faecal specimens from 628 newborn babies in the nurseries of six metropolitan hospitals were examined by electron microscopy for rotaviruses. 304 babies (49%) were found to be excreting virus. all those infected were in five nurseries; viruses were not detected in specimens from the sixth nursery. two nurseries were studied for 9 mo and another for 11 mo and rotaviruses were found consistently in 40-50% of stools examined. there was no seasonal variation. none of the neonates under the age of on ... | 1977 | 73060 |
| ribonucleic acid polymerase activity associated with purified calf rotavirus. | the presence of an rna-dependent rna polymerase is demonstrated in purified rotavirus particles. optimum polymerase activity was found between 45 to 50 degrees c, at ph 8, and in the presence of 10 mm-magnesium ions. the polymerase product was highly sensitive to pancreatic rnase (97%) in low or high salt concentration. the enzyme was activated by edta treatment of intact particles or heat shock. the similarities between reovirus, blue-tongue virus and rotavirus polymerases are discussed. | 1977 | 21225 |
| plaque assay of neonatal calf diarrhea virus and the neutralizing antibody in human sera. | neonatal calf diarrhea virus (a bovine rotavirus) formed distinct plaques in monolayers of ma-104 cells, an established macacus rhesus monkey kidney cell line, when diethylaminoethyl dextran and trypsin were included in the overlay medium. by using this plaque assay method, titration of neutralizing antibody to neonatal calf diarrhea virus was made feasible. it was demonstrated that some human sera contained neutralizing antibody to this agent. | 1977 | 188863 |
| [rotavirus: a frequent cause of acute gastroenteritis in children]. | 1977 | 189452 | |
| immune electron microscopy of transmissible gastroenteritis virus and rotavirus (reovirus-like agent) of swine. | immune electron microscopy (iem) was developed as a diagnostic aid for detecting and identifying transmissible gastroenteritis virus and rotavirus (reovirus-like agent) in fecal and intestinal contents from cases of gastroenteritis in young pigs. variables involved in use of direct iem and its sensitivity were determined. aggregates of virus coated with specific antibody were seen in virus samples mixed with homologous convalescent antiserum, but not in control samples containing preexposure ser ... | 1977 | 189646 |
| diarrhoea in pigs induced by rotavirus. | electron microscope examination of the faeces of scouring pigs revealed virus particles which were morphologically indistinguishable from rotavirus (reo-like), a virus associated with diarrhoea in neonatal pigs (lecce, king & mock, 1976). this is the first record of the virus in the republic of south africa. | 1977 | 206862 |
| epidemic gastroenteritis in children associated with rotavirus infection. | 1977 | 204575 | |
| rotaviruses in venezuelan children with gastroenteritis. | rotaviruses were identified by electron microscopy in 19 of 26 (73%) venezuelan children with gastroenteritis. noncultivable adenoviruses and small (29-30 nm), spherical viruses were also observed in a few cases. bacterial pathogens were isolated from five children (19%), all of whom were also carrying rotaviruses. | 1977 | 842776 |
| biochemical studies on a reovirus-like agent (rotovirus) from lambs. | ten polypeptides were detected in double-capsid lamb rotavirus; four of these appeared to be associated with the outer capsid. lamb rotavirus rna, which consisted of 11 or 12 segments, differed from pig rotavirus rna in the electrophoretic mibility of one of the genome segments. | 1977 | 850308 |
| rotavirus infections in calves. ii. experimental infections with a dutch isolate. | rotaviruses were observed electron microscopically in clarified faecal samples from calves with diarrhoea. several herds with a history of scouring among neonatal calves were shown to be infected with rotaviruses. a bacteria-free faecal filtrate from a rotavirus positive sample administered orally induced diarrhoea in one out of two colostrum-deprived calves. both calves excreted rotaviruses in their faeces and serum antibody responses were demonstrated by complement-fixation and indirect immuno ... | 1977 | 857337 |
| propagation of the rotavirus of neonatal calf diarrhea in fetal intestinal cell cultures. | bovine fetal intestinal cells were successfully propagated in monolayer cultures for up to 21 passages. infection of these cells with the rotavirus of neonatal calf diarrhea resulted in a cytopathic effect that was more obvious than in infected bovine fetal kidney cells. | 1977 | 861843 |
| solid-phase radioimmunoassay for the detection of rotavirus. | a solid-phase radioimmunoassay method has been developed for the detection of rotavirus in the form of a purified antigen and in stool. the parameters of the radioimmunoassay were examined and optimized to give high sensitivity and same-day results. compared with electron microscopy, the assay is up to 10 times as sensitive for detection of the virus in stool and up to 128 times as sensitive for detection of a purified virus antigen. in a field study on stool specimens it was at least as effici ... | 1977 | 863510 |
| a survey of rotaviruses in sheep in scotland. | 1977 | 867760 | |
| clinical, laboratory, and epidemiologic features of a viral gastroenteritis in infants and children. | we studied 27 infants admitted to the hospital with acute diarrhea caused by human rotavirus (hrv) and obtained additional data on fecal excretion from ten outpatients with the same infection. the disease was characterized by watery diarrhea with fever and vomiting at the onset, isotonic dehydration, compensated metabolic acidosis, and increased concentrations of sodium and chloride but low concentrations of sugar in stools. diarrhea usually ceased in three to four days when oral feedings were r ... | 1977 | 887336 |
| [seroepidemiological investigations on the epidemiology of human rotavirus infections (author's transl)]. | 263 sera of infants and children up to 10 years of age were tested for antibodies (cf-test) against nebraska calf diarrhoea virus (ncdv) which is antigenically related to the rotavirus of man; in addition the mean antibody titers in different age groups were investigated. antibodies of maternal origin were eliminated during the first year of life. most of the infections occurred during the second year of life and at the end of this year 40-50% of the investigated children demonstrated antibodies ... | 1977 | 899370 |
| [infectious agents in diarrhea of hospitalized children in costa rica]. | shigella, toxigenic escherichia coli (stable toxin) and rotaviruses were frequently found among 50 children hospitalized with diarrhea studied during one year. these agents were less common among 45 controls without diarrhea, of comparable age and from the same wards as the cases reported. there was a greater frequency of respiratory symptoms in the diarrhea associated with rotaviruses. these were characterized by frequent bowel movements and vomiting and often fever. the bacterial diarrheas sho ... | 1977 | 911458 |
| diarrhea in calves: diagnosis and incidence in the north central states. | 1977 | 220632 | |
| review of rotavirus infections of man and animals. | 1977 | 220639 | |
| [diarrhea outbreak and bacterial resistance to ampicillin in newborns]. | twenty-eight healthy neonates from the san juan de dios hospital were studied to determine the pattern of antibiotic resistance of indigenous intestinal bacteria. sixty-eight per cent of infants had enterobacteriaceae resistant to several wide-spectrum antibiotics, including ampicillin; 28 per cent of the cultures had plasmid-mediated ampicillin resistance. in the course of the study, an outbreak of 10 acute cases of diarrhea occurred, not associated to any of the commonly known agents, includin ... | 1977 | 242927 |
| a microbiological investigation of acute summer gastroenteritis in black south african infants. | a microbiological investigation of black infants suffering from severe acute summer gastroenteritis revealed enteropathogenic agents in 30 out of 37 patients (81%). enterotoxigenic bacteria were isolated from 15 patients (41%). a total of 16 enterotoxigenic strains were isolated, comprising 9 enterotoxigenic escherichia coli strains secreting labile and stable toxin on their own and in combination, and labile-toxin secreting strains of klebsiella pneumoniae (4), enterobacter cloacae (2) and prot ... | 1977 | 325127 |
| gastroenteritis in children: a two-year review in manitoba. i. etiology. | during two years, 1,217 children hospitalized with gastroenteritis at the children's centre in winnipeg, manitoba, canada were studied. bacterial pathogens were present in 25% of these children: enteropathogenic escherichia coli in 120, shigella in 139, salmonella in 24, and multiple pathogens in 18. rotavirus was detected in 54 (11%) of 472 patients examined. rotavirus and enteropathogenic e. coli were the most common pathogens in infants, and shigella was the most common in older children. bac ... | 1977 | 330769 |
| a microbiological study of gastro-enteritis in black infants. | a survey was carried out to determine the aetiological agents responsible for acute gastro-enteritis in black infants. bacteriological examination of 191 patients and 178 age-matched controls showed that salmonellae and shigellae together accounted for only 15% of cases and were harboured by almost 7% of controls. seropositive 'enteropathogenic' escherichia coli was an important cause of diarrhoea, especially in the summer months. enterotoxin-producing e. coli was not a significant cause of gast ... | 1977 | 331509 |
| reovirus-like agent in acute epidemic gastroenteritis in japanese infants: fecal shedding and serologic response. | the reovirus-like agent, sometimes referred to as duovirus or rotavirus, was visualized by electron microscopy in stool extracts from japanese infants and young children with acute epidemic gastroenteritis. the virus particles measured 70 nm in diameter and had double-shelled capsids. one hundred ten (89%) of 124 patients with the gastroenteritis had such virus particles in stools obtained during the acute phase. the virus particles were excreted in the stools usually during the first eight days ... | 1977 | 402427 |
| diagnosis of rotavirus infection by cell culture. | rotaviruses were detected by electronmicroscopy in 35 of 84 specimens of faeces from infants with diarrhoea, and in 31 by fluorescent staining of tissue cultures infected with help of centrifugation. llc-mk2 cells were found to be the most sensitive, although primary and secondary human-embryo-kidney and primary calf-kidney cells could also be used. a micromodification of the tissue-culture method provides a relatively simple technique for the diagnosis of rotavirus infection, for the titration ... | 1977 | 402472 |
| complement-fixing antibody response to rotavirus infection. | a human rotavirus complement-fixing (cf) antigen, prepared by purification of large volumes of fluid feces collected from children with winter diarrhea, was used to study the development and persistence of antibody in children with diarrhea and the prevalence of rotavirus antibody in melbourne. in children with diarrhea, antibody rises were detectable within 4 to 6 weeks of the onset of illness, and the titers usually remained elevated for the next 1 to 2 years. cf antibody did not develop in tw ... | 1977 | 403196 |
| stool viruses in babies in glasgow. i. hospital admissions with diarrhoea. | stools from 183 babies under 2 years of age admitted to ruchill hospital with diarrhoea were examined by electron microscopy, virus culture, bacterial culture and light microscopy. as far as possible, several stools were examined from each patient and the results showed rotaviruses, astroviruses and other viruses in association with symptoms, as well as the expected bacterial pathogens. examination of several stools from the same patient also showed that in this age group the viral flora of the ... | 1977 | 403233 |
| rapid diagnosis of rotavirus infections: comparison of electron microscopy and immunoelectroosmophoresis for the detection of rotavirus in human infantile gastroenteritis. | eighty-seven faecal samples from infants and children suffering from acute gastroenteritis were investigated for the presence of rotavirus by immunoelectroosmophoresis (ieop) and electron microscopy (em). sixty-one % of the samples contained rotavirus antigens when examined by ieop whereas only 50% were diagnosed as positive em. however, where it was possible to perform em within the same day that the sample was received it took 24 h to establish the diagnosis by ieop. the high sensitivity of th ... | 1977 | 406356 |
| aetiology of acute gastroenteritis in infancy and early childhood in southern india. | the aetiology of acute gastroenteritis was studied in 50 infants and young children. bacterial pathogens were isolated in 33, enteropathogenic e. coli (epec), salmonella, and shigella being the commonest isolates. rotaviruses were detected in the stools of 13 of the cases. all children with gastroenteritis in whom rotavirus was detected were seen during the months july to december. in 30 children who served as controls, epec were isolated in 6, but rotavirus was detected in none. it is concluded ... | 1977 | 406857 |
| demonstration of size variation of rna segments between different isolates of calf rotavirus. | polyacrylamide gel electrophosesis of rna extracted from wild rotavirus isolates and cell culture adapted virus revealed a significant variation in the molecular weight of individual rna segments. the major differences were observed between wild isolates on the one hand and the adapted strain on the other hand. the slight variations that were observed between different wild isolates were found regularly and appeared to be related to the origin of the samples. | 1977 | 407327 |
| [rotavirus and gastroenteritis in the newborn infant. initial observations]. | a simplified method for the diagnosis of rotavirus by electron microscopy is presented. the incidence of these viruses is observed in new-born infants and the relationship with acute gastroenteritis is recalled. | 1977 | 408044 |
| viruses and diarrhea--a review. | a critical analysis of the literature shows that only a few viral agents have been proved by accepted criteria to cause acute gastroenteritis. rotavirus, of the rna family reoviridae, is the cause of most childhood gastroenteritis. the parvovirus-like agents (norwalk, hawaii, mc, and w) are proved agents of epidemic gastroenteritis. there is good evidence that the marcy, fs, and niigata agents were the causes of gastroenteritis outbreaks. thirty percent of all acute gastroenteritis episodes in l ... | 1978 | 343580 |
| a prospective study of enteropathogenic escherichia coli in endemic diarrheal disease. | the rate of isolation of escherichia coli belonging to the traditional serotypes enteropathogenic for infants was studied prospectively in two groups. group 1 consisted of children with diarrhea and of controls without gastrointestinal disease who were matched for age and inpatient or outpatient status. group 2 consisted of families entered in a prospective study of rotavirus infections. in group 1 enteropathogenic escherichia coli were found in 13 (6%) of 220 children younger than 12 months of ... | 1978 | 344810 |
| interventions in diarrheas of infants and young children. | to prevent diarrhea, efforts must be directed toward decreasing environmental contamination of enteropathogens, altering exposure of persons to naturally occurring agents, or to increasing host tolerance to prevalent enteropathogens. a vaccine is feasible in the control of certain enteric infections among high risk groups: typhoid fever and cholera, in areas of hyperendemicity or during an epidemic, shigellosis in institutionalized populations that are at risk to develop illness due to known pre ... | 1978 | 359524 |
| breast-feeding: main promoter of infant health. | unique immunological factors in breast milk are responsible for the protection it affords infants against pathogenic agents. this protection is particularly effective against the infectious agents which invade or colonize the small and large intestine. factors interfering with enterotoxigenic and enteroinvasive bacteria, and with entero- and rotaviruses, have already been detected in colostrum and mature human milk. moreover, the biochemical composition of human milk makes it the best infant foo ... | 1978 | 362883 |
| rotavirus gastroenteritis in hospitalized infants and young children in mexico city. | 1978 | 228369 | |
| routine diagnosis of human rotaviruses in stools. | electron microscopy, immune electron microscopy and complement fixation as methods of detecting rotavirus in the stools of young children with gastroenteritis were compared in a blind study during the winter of 1975-6. complement fixation was the simplest to perform, was as sensitive as the other two, and allowed a quantitative measurement of viral excretion. absorption of faecal extracts with fetal calf serum usually removed the anticomplementary activity of faecal extracts. | 1978 | 204665 |
| rotavirus in travelers' diarrhea: study of an adult student population in mexico. | the role of rotavirus in adult diarrhea was evaluated in 165 students attending a mexican university. students were divided into three groups: newly arrived summer students from the united sttes, regular students from the united states, and mexican and venezuelan students. ninety-one students with diarrhea and 74 corresponding, matched, asymptomatic control students were included in the study. the frequency of rotavirus in stools was determined by electron microscopy with use of the pseudoreplic ... | 1978 | 204710 |
| elisa: enzyme-linked immunosorbent assay. | similar in design to radioimmunoassay, comparable in sensitivity and specificity but easier, safer, and less expensive, this new diagnostic technique uses enzyme-labeled rather than isotope-labeled reagents. the end point is a color change that can be assessed by colorimetry or with the naked eye. various techniques of elisa are described, along with examples of current and potential clinical applications. | 1978 | 215505 |
| prevalence of antibody to the norwalk agent by a newly developed immune adherence hemagglutination assay. | an immune adherence hemagglutination assay (iaha) for the detection of antibody to the norwalk agent of acute epidemic nonbacterial gastroenteritis was developed using as antigen virus purified from stool from an experimentally infected volunteer. the assay was sensitive and specific and was efficient for detecting norwalk antibody seroresponses. the prevalence of norwalk antibody in various groups in the united states was studied. antibody to the norwalk agent was acquired gradually, beginning ... | 1978 | 215713 |
| prevalence of rotavirus antibody in infants and children. | 1978 | 217819 | |
| machakos project studies: agents affecting health of mother and child in a rural area of kenya. vii. the incidence of diarrhoeal disease in the under-five population. | the outcome of three years of fortnightly diarrhoea surveillance of about 4000 children under the age of five is described. the two-weekly incidence of diarrhoea in children in the age group 0-5 months, 6-11 months and 12-23 months, who were reported and/or observed to be ill, was 3.4%, 5.6% and 3.4% respectively. a decline in the attack rates of diarrhoea not associated with measles at the end of 1975 and the beginning of 1976 corresponds with a similar decline in the incidence of measles and m ... | 1978 | 216135 |
| [detection of an agent similar to rotavirus in infantile gastroenteritis]. | the etiology of nonbacterial enterovirus infections in children was studied in autumn and winter under conditions of focal outbreaks in moscow hospitals. electron microscopy revealed a reovirus-like agent in concentrated suspensions of feces collected in the acute period of the disease from children suffering from diarrhea. examinations of paired sera from the patients by the cf test with the antigen of nebraska calf disease virus established significant rises in antibody titers in 68.3% of case ... | 1978 | 216151 |
| [rotavirus gastroenteritides in infants and toddlers]. | 1978 | 216499 | |
| simplified radioimmunoassay for detection of human rotavirus in stools. | a simplified radioimmunoassay (ria) technique was developed to facilitate the diagnosis of human rotavirus in stools of infants with diarrhea. this microtiter solid-phase ria utilizes as a critical reagent hyperimmune serum against a tissue culture-grown simian rotavirus that is immunologically closely related to the human agent. one hundred thirteen pediatric stool samples were tested. thiry-five specimens known to be positive for rotavirus by electron microscopy were also positive by ria. all ... | 1978 | 216754 |
| production of calf rotavirus hemagglutinin inhibitors in the infected cell culture fluid. | 1978 | 216892 | |
| the effect of vaccination on titres of antibody to rotavirus in colostrum and milk. | the continued presence of antibody to rotavirus in the gut of the neonatal ruminant has been shown to be important in protecting against clinical disease associated with rotavirus infections. this effect is dependant upon the amount of antibody included in the diet. titres of antibody to rotavirus may be relatively high in ruminant mammary secretions immediately after parturition but decline rapidly over the course of the first day. the influence of vaccination on the titres of antibody, the con ... | 1978 | 218490 |
| the influence of colostrum on neonatal rotaviral infections. | 1978 | 218491 | |
| the detection of rotavirus specific antibody in colostrum and milk by elisa. | the blocking method of elisa for the detection and titration of rotavirus-specific antibody in colostrum is described. the results obtained were positively correlated with those of a neutralizing antibody test. on one farm colostrum samples were obtained over a period of 18 months. no relationship was found between the titer of colostrum obtained shortly after calving, and the development of rotavirus-associated diarrhoea in calves. on a second farm only samples obtained during the calving seaso ... | 1978 | 218492 |
| enteropathogenic organisms. new etiologic agents and concepts of disease. | 1978 | 215856 | |
| [rota virus infantile gastroenteritis: clinical and epidemiological study]. | 1978 | 218164 | |
| the experimental production of diarrhoea in colostrum deprived axenic and gnotoxenic calves with enteropathogenic escherichia coli, rotavirus, coronavirus and in a combined infection of rotavirus and e. coli. | we attempted to produce diarrhoea experimentally in the newborn calf by orally injecting 17 colostrum-deprived calves with two serotypes of escherichia coli ent+ k99+, a rotavirus and a coronavirus. with e. coli alone, a dose of 2 x 10(8) bacteria administered 24 hours after birth causes a mild attack of diarrhoea, whereas 1 x 10(10) bacteria leads to dehydration and death. an inoculation of rotavirus is followed by diarrhoea which always contains large quantities of rotavirus. these animals wer ... | 1978 | 219751 |
| scanning electron microscopy of abomasium and intestine of gnotoxenic calves infected either with rotavirus, coronarivus or enteropathogenic escherichia coli or with rotavirus and e. coli. | neonatal calf diarrhoea induced with several agents of infection was studied by scanning electron microscopy. in a gnotoxenic calf infected with e. coli k99+ ent+, slight lesions of the small intestine were observed and desquamation or puffiness of microvilli occurred. in rotavirus-infected calves, the abomasum was covered with abudant mucous film and appeared to be desquamated. in the small intestine, no desquamation of epithelium was observed. inoculation of the rotavirus and e. coli induced s ... | 1978 | 219752 |
| [corona- and rotavirus enteritis in newborn calves in bulgaria]. | in the course of investigations into enteritis enzootics in newly-born calves, 55 fecal samples have been studied through direct electron microscopy by the negative contrast method. rota viruses were found in eight of them, corona viruses in 38.2 per cent of the cases, and both rota- and corona viruses in 49.1 per cent of the cases. two types of rota-viral particles were observed: with and without supercapsids. also two types of corona-viral particles were established. the first type was relativ ... | 1978 | 223276 |
| [pediatrics in 1977]. | 1978 | 206954 | |
| prevalence of antibodies to a rotavirus in black and white populations in south africa. | a serological survey of antibodies to a rotavirus in 252 sera from black and white south africans was performed by means of an immunofluorescence test using cells infected with simian rotavirus, sa 11, as antigen. the results indicate widespread presence of this agent in south africa and also that the black population is exposed to a greater extent than the white population. | 1978 | 206980 |
| nosocomial rotavirus gastroenteritis in a neonatal nursery. | 1978 | 206997 | |
| intestinal damage in rotavirus infected calves assessed by d-xylose malabsorption. | 1978 | 207007 | |
| isolation of rotavirus from calves in the federal republic of germany. | 1978 | 207058 | |
| [rotavirus as a causative agent of infantile gastroenteritis. diagnosis and epidemiology (author's transl)]. | some years ago it was not possible in spite of intensive bacteriological and virological work on the etiology of acute infantile gastroenteritis to find any agent that may readily be linked to this disease. since five years now early sporadic reports about virus-like particles have proved worldwide to be correct and in the meantime have substantially been supported by new findings in that these particles are the main causative agent of infantile gastroenteritis. as part of our electron-microscop ... | 1978 | 207634 |
| human rotavirus and its antibody: their coexistence in feces of infants. | rotavirus and its antibody were detected by paper disk solid-phase radioimmunoassay or electron microscopy in feces of infants and young children with acute diarrhea. the fecal specimens in which rotavirus was detectable often contained a high titer of antibodies, which were shown by radioimmunoassay to belong mainly to the immunoglobulin g class. rotavirus was rarely detected in the specimens containing immunoglobulin a antibodies. by dissociation tests carried out by radioimmunoassay, it was s ... | 1978 | 207717 |
| rna polymerase associated with human rotaviruses in diarrhea stools. | rna polymerase activity was detected in six stools which were partially purified by high-speed centrifugation from infants with rotavirus gastroenteritis, but was not detected in five stools which were negative for rotavirus by counterimmunoelectrophoresis and radioimmunoassay. the polymerase activity was associated with the 1.38-g/ml rotavirus band after purification in a cscl gradient. | 1978 | 207902 |
| a survey of rotavirus associated with gastroenteritis in aboriginal children in western australia. | in a year-long survey which was carried out between april, 1975, and march, 1976, faecal specimens from aboriginal and some non-aboriginal children who suffered from gastroenteritis were examined by electron microscopy for rotavirus, and also tested for bacteria and parasites. the children were under six years of age and came from all parts of western australia, except perth. rotaviruses, "astroviruses" and adenoviruses were detected as well as the usual potentially pathogenic bacteria and paras ... | 1978 | 207954 |
| enzyme-linked immunosorbent assay for identification of rotaviruses from different animal species. | rotaviruses cause gastroenteritis in man and a wide variety of animal species. they cross-react in many immunologic tests and have a similar appearance by electron microscopy, making differentiation among them difficult. rotaviruses derived from different host species were distinguished by postinfection serum blocking virus activity in an enzyme-linked immunosorbent assay (elisa). thirty-three rotavirus isolates from children living in three different parts of the world could not be differentiat ... | 1978 | 208150 |
| pathological changes in the small intestine of neonatal calves naturally infected with reo-like virus (rotavirus). | nine calves, of which six had been challenged with an enteropathogenic strain of escherichia coli, were found to be naturally infected with rotavirus. rotavirus was recovered from the faeces of six calves and rotavirus antigen was detected in the intestinal mucosa of all calves. stunting and fusion of villi were seen principally in the proximal and middle small intestine, where rotavirus antigen was detected by immunofluorescence. typical lesions of enteric colibacillosis were found in the dista ... | 1978 | 208222 |
| comparison of human and animal rotavirus strains by gel electrophoresis of viral rna. | 1978 | 208274 | |
| rotavirus gastroenteritis. | 1978 | 208471 | |
| diagnosis of viral agents associated with neonatal calf diarrhea. | during this study, 134 samples have been examined for the detection of the viruses associated with neonatal calf diarrhea. the presence of nebraska viruses (rotavirus and coronavirus) has been demonstrated by using the electron microscope and the fluorescent antibody techniques while the presence of other viruses has been detected by the observation of a cytopathic effect on monolayer cells of calf testis. the nebraska viruses have been demonstrated in 107 (80%) out of 134 field case specimens. ... | 1978 | 208735 |
| noncultivable viruses and neonatal diarrhea: fifteen-month survey in a newborn special care nursery. | during a 15-month period of surveillance, diarrhea developed in 257 of 913 babies (28%) admitted within 2 hours of birth to a special care nursery in melbourne, australia. diarrhea was seasonal, affecting a maximum of 43% of babies admitted during one winter month (july) and a minimum of 13% of babies admitted during one summer month (december). diarrhea was no more frequent nor more severe in babies of very low birth weight or of very early gestational age. two noncultivable viruses were locate ... | 1978 | 209058 |
| oral hydration rotavirus diarrhoea: a double blind comparison of sucrose with glucose electrolyte solution. | of 57 male children, aged 5 months to 2 1/2 years with rotavirus diarrhoea, 28 were given oral therapy with sucrose electrolyte solution and 29 were given glucose electrolyte solution in a randomised double-blind trial. all were rehydrated and remained so on oral therapy alone. these patients were compared with 44 children, also with rotavirus, who were treated only with intravenous hydration. the oral therapy and intravenous therapy groups did not differ clinically in the rate of rehydration or ... | 1978 | 209263 |
| effect of serum albumin on hemagglutination with nebraska calf diarrhea virus. | 1978 | 209292 | |
| [identification of rotavirus by counter-immunoelectrophoresis in cases of infantile diarrhea]. | 1978 | 209531 | |
| antibody to rotavirus in dogs and cats. | 1978 | 209605 | |
| epidemiological aspects of rotavirus infection in hospitalized venezuelan children with gastroenteritis. | the prevalence of rotavirus infection in hospitalized venezuelan children with gastroenteritis was studied during the period november 1975 to december 1976. rotaviruses were the pathogens most frequently associated with gastroenteritis, being found in 121 of 293 (41.3%) patients and in only 3 of 66 (4.5%) controls. other viruses (adenoviruses, enteroviruses, and small icosahedrical viruses) were detected at a lower frequency both in cases and controls. rotaviruses were detected at a lower freque ... | 1978 | 209696 |
| rotavirus infections in adults in association with acute gastroenteritis. | during an epidemic of acute gastroenteritis in helsinki, in march--may 1976, 18 out of 40 adult patients showed electron microscopic and/or serologic evidence for rotavirus infection. rotavirus was most frequently seen in the fecal suspensions from 2 to 6 days after the onset of the symptoms but persisted in one patient for as long as 10 days. an increase in the complement-fixing (cf) serum antibody titers against the related nebraska calf diarrhea virus (ncdv), or an initially high titer and su ... | 1978 | 210251 |
| serial studies of virus multiplication and intestinal damage in gnotobiotic piglets infected with rotavirus. | a serial study of rotavirus infection in gnotobiotic piglets is described. they were infected when 7 days old and the course of infection was followed for 21 days, by immunofluorescence and histological examinations of the intestinal epithelium and by titration of the virus content of the gut lumen. there appeared to be two stages of recovery of the intestinal wall, the earlier stage involving non-immune mechanisms and the later invloving antibody. | 1978 | 210280 |
| [rotavirus infections]. | 1978 | 210442 | |
| studies on cross protection induced in calves by rotaviruses of calves, children and foals. | inoculation at birth with a live attenuated strain of a bovine rotavirus isolated in the usa (scourvax-reo) induced protection in five gnotobiotic calves seven to 21 days later against a uk isolate of pathogenic bovine rotavirus. however, no protection was induced in three calves challenged three to five days after vaccination. there was a close antigenic relationship demonstrated between the two bovine rotavirus isolates. in contrast only one of three gnotobiotic calves inoculated with foal rot ... | 1978 | 210557 |
| comparison of human rotavirus disease in tropical and temperate settings. | human rotaviruses (hrv) are a common cause of acute nonbacterial gastroenteritis in pediatric patients. a prospective study of hrv disease in a temperate (dallas) and a tropical (san jose, costa rica) setting demonstrated differences in seasonal distribution. in both locales, hrv accounted for 50% to 60% of acute nonbacterial gastroenteritis episodes from december through february; this period corresponded to the cooler months of winter in dallas and to the dry season in san jose. during the res ... | 1978 | 210656 |
| diarrhoea in piglets inoculated with rotavirus. | a rotavirus isolated from a field outbreak of diarrhoea in artificially reared piglets was purified, filtered and administered orally to gnotobiotic and conventional piglets. four successive passages of the virus in gnotobiotic piglets produced severe diarrhoea within 20 to 24 hours of administration. the diarrhoea lasted several days causing dehydration, emaciation, loss of body weight and some deaths. virus was demonstrated in the faeces of the infected piglets by electron microscopy. conventi ... | 1978 | 210755 |
| [role of bacteria and viruses in the etiology of acute gastroenteritis in children]. | 1978 | 211023 | |
| panel report on the colloquium on selected diarrheal diseases of the young. | 1978 | 211109 | |
| the effect of trypsin on the growth of rotavirus. | it has been found that 1000-fold more bovine rotavirus is obtained when trypsin is incorporated in the maintenance medium and allowed to remain throughout the growth cycle. this holds true for primary calf kidney (ck) cells and also for several continuous and semi-continuous cell lines. in the presence of trypsin it has been possible to pass the virus serially on continuous cell lines seven times. concentrations of 1 to 10 microgram/ml of trypsin are found to be effective. preliminary results su ... | 1978 | 211180 |
| prospective study of enteropathogens in children with diarrhea in houston and mexico. | during a 22-month period, 595 children with diarrhea and 210 age-matched controls attending clinics in houston (367 children) and mexico (438) were prospectively evaluated for enteric pathogens. enteropathogens associated with disease were shigella (18%), rotavirus (14%), salmonella (9%), toxigenic escherichia coli (6%), and others (12%), including 14 proteus isolates that caused rounding of adrenal cells. enteropathogens were isolated from a greater (p less than 0.001) number of children with d ... | 1978 | 211215 |
| a clinical study of rotavirus gastroenteritis. | 1978 | 211217 | |
| rotavirus (reovirus-like) infection of neonatal ruminants in a zoo nursery. | an outbreak of a pneumoenteric disease occurred in neonates in a zoo nursery. four of seven affected animals died. rotaviruses were observed in the feces of an affected 4-day old impala (aepyceros melampus), a thomson's gazelle (gazella thomsonii) and an addax (addax nasomaculatus). encapsulated escherichia coli also were isolated from the feces. the recovered rotaviruses was antigenically related to bovine rotavirus. a bovine rotavirus vaccine was given orally and no adverse effects were noted. | 1978 | 211273 |
| [viruses and children's diarrhoea]. | 1978 | 211647 | |
| epizootiology of bovine rotavirus infection. | published information on rotaviruses as pathogens, the source of virus infection and the method of transmission of infection under normal conditions are reviewed. the antigenic differences between rotavirus isolates from children, calves, pigs, foals and mice are discussed. bovine rotaviruses isolated in the usa and the uk were shown to be closely related antigenically and the us vaccine strain protected calves from challenge with the uk rotavirus. nineteen normally reared calves, with 20 or mor ... | 1978 | 211696 |
| antibody titres to lamb rotavirus in colostrum and milk of vaccinated ewes. | ewes were vaccinated two to three weeks prior to mating with a formalin-treated preparation of lamb rotavirus. the colostrum and milk produced by vaccinated ewes after the subsequent pregnancy were shown to contain significantly higher titres of antibody to the virus than did mammary secretions from non-vaccinates. the virus neutralising antibody activity was associated with igg in both colostrum and milk. however, igg concentrations in the mammary secretions of vaccinates and non-vaccinates did ... | 1978 | 211697 |