Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| large round cell granulomas induced by murine sarcoma virus (moloney) in mouse brains. | the effect of murine sarcoma virus of moloney strain on central nervous system was examined morphologically in swiss mice of different age. a single intracranial inoculation of cell-free virus solution resulted in the induction of characteristic intracerebral granulomas in 82.8% of the newborn to 5 day-old group, in 71.4% of the 6 to 10 day-old group, and in 68.0% of the 11 to 20 day-old group. the mean latency periods to tumor recognition were 16.5, 21.1, and 33.5 days, respectively. the granul ... | 1975 | 128993 |
| tumor inhibiting capacity of spleen and lymph node cells from mice with murine sarcoma virus (msv-m)-induced tumors. | the direct tumor inhibiting capacity of spleen and lymph node cells obtained from mice in different stages of moloney sarcoma virus (msv-m)-induced tumor development was studied. msv-m producing ascitic tumor cells with a tumor dosis50 of about 10(3) cells and a mean latent period--depending on the amount of transplanted cells--of6-12 days were used as target cells. they were mixed with dilutions of spleen and lymph node cells obtained from mice at various times after infection with msv-m. the c ... | 1975 | 181922 |
| primary and secondary in vitro generation of cytolytic t lymphocytes in the murine sarcoma virus system. | cell-mediated cytotoxic responses in vitro to surface antigens associated with murine sarcoma virus (msv)-induced tumors were investigated using mixed leukocyte-tumor cell cultures (mltc). the source of responding cells was either spleens from normal c57bl/6 mice (primary mltc) or spleens of c57bl/6 mice carrying or having rejected a msv-induced tumor (secondary mltc). graffi virus-induced gil-4 leukemia cells, rauscher virus-induced rb1-5 leukemia cells, and msv-induced msv-b16 sarcoma cells we ... | 1975 | 185057 |
| studies on the distribution and origin of cytolytic t lymphocytes present in mice bearing moloney murine sarcoma virus (msv)-induced tumors. | 1975 | 1085620 | |
| cell-mediated immunity to moloney sarcoma virus in mice. ii. analysis of antigenic specificities involved in t lymphocyte-mediated in vivo rejection of murine sarcoma virus-induced tumors. | sublethally irradiated balb/c mice innoculated with moloney sarcoma virus (msvm) develop progressively growing tumors and die within 30 days of virus innoculation. these animals can be protected from tumor progression (and death) by innoculation of small numbers of msv-immune t lymphocytes from msv-m innoculated (but unirradiated) animals. t lymphocytes in these donor animals have been shown to express immunity to a variety of viral and virally-induced antigens. we have investigated whether immu ... | 1975 | 1086221 |
| enhancement of moloney sarcoma virus-induced tumor growth by concanavalin a. | adult mice injected intraperitoneally with concanavalin a (con-a) commencing with the onset of 6mm. moloney sarcoma virus (msv)-induced tumors showed a marked enhancement of tumor growth compared to untreated control mice; whereas, treatment with con-a beginning on day 0 relative to msv inoculation had no effect. antibody production as measured by indirect membrane fluorescence was not affected by con-a. since in vivo administered con-a has a specific suppressive effect on t-lymphocytes it is su ... | 1975 | 1090515 |
| antibody production and interaction with lymphoid cells in relation to tumor immunity in the moloney sarcoma virus system. | the temporal development of antibodies to moloney sarcoma virus (msv)-induced antigens in relation to tumor progression was followed with the membrane immunofluorescence (mf) and antibody-dependent normal lymphocyte cytotoxicity (adnlc) assays. antibody was detected at 14 days following virus infection by mf. in mice that developed primary tumors which regressed, mf titers developed to high levels following a period between 2 and 4 weeks post-innoculation during which the titers remained at low ... | 1975 | 1090675 |
| antibody-dependent lymphocyte cytotoxicity in the murine sarcoma virus system: activity of igm and igg with specificity for mlv determined antigen(s). | antisera with specificity for moloney leukemia virus-(mlv) determined antigen(s) were studied for their ability to induce mlv antigen bearing target cell reduction by lymphocytes in microcytotoxicity assays. sera from animals which had regressed moloney sarcoma virus (msv) tumors as well as sera from animals with progressively growing msv tumors were found to induce normal lymphocytes to be active against the targets. regressor serum was found also to induce cytotoxicity by immune lymphocytes fr ... | 1975 | 1117138 |
| prostaglandin synthesis inhibition: effect on bone changes and sarcoma tumor induction in balb/c mice. | an increase in prostaglandins (pgs) of the e series has been demonstrated in moloney sarcoma virus (msv)-induced leg tumors of 6-week-old balb/c male mice. the level of the hormone has been shown to increase with the tumor diameter and decrease with tumor regression. at the peak of tumor size the tibial bones of the mice were considerably deformed, suggesting osteoclastic activity. the systemic calcium level was not elevated, indicating possible release of calcium into the local tumorous area. i ... | 1975 | 1140869 |
| [histopathologic and ultrastructural study of tumors induced by murine sarcoma virus (msv) (author's transl)]. | various growth phases of moloney murine sarcoma virus (m-msv) induced tumors in suckling and young adult balb/c mice have been studied by light and electron microscopy. in the early phase (3-6 days following m-msv), observations at the injection site of the thigh muscles consisted of endo- and perimysial edema "activated" muscle satellite, cells, endothelial cells and fibroblasts, scattered type c virus particles within the muscle fibers, muscle fibers and endomysial cells undergoing necrosis an ... | 1975 | 1226574 |
| search for nucleic acid sequences complementary to a murine oncornaviral genome in poly(a)-rich rna of human leukemic cells. | the presence of viral-like sequences in the rna of various types of leukemic cells was investigated by hybridizing cellular poly(a)-containing rna with cdna synthesized in an endogenous system of purified moloney murine sarcoma virus [m-msv-(mlv)]. poly(a)-rna-cdna hybrids were detected by assaying their resistance to s1 nuclease. hybrids were found in 22 out of the 46 leukemias that were tested. none of the controls, including material obtained from buffy coats, bone marrow cells, and a continu ... | 1975 | 1061078 |
| tumor induction by moloney sarcoma virus in athymic nude mice. | athymic nude (nu/nu) mice, normal nmri (plus/plus) mice, and nu/plus heterozygotes, inoculated at birth with the moloney strain of murine sarcoma virus (m-msv), did not differ either in tumor growth rate or incidence or in mortality due to tumor development; neither did nude (nu/nu) and normal nmri mice inoculated with m-msv at 30 days of age differ in the initial tumor growth rate. however, tumors induced in adult nude mice grew progressively and eventually killed the hosts, whereas tumors indu ... | 1975 | 1078700 |
| the relationship between tumorigenicity, growth in agar and fibrinolytic activity in a line of human osteosarcoma cells. | the te-85 osteosarcoma cell line has several of the in vitro properties of malignant cells, including colony formation in agar, but has low extracellular fibrinolytic activity and no capacity to form tumors in ats-treated hamsters. some te-85 cells clones (clones 2, 4 and 6) have increased fibrinolytic activity but do not form tumors in hamsters. te-85 cells infected with mammalian transformation-defective viruses have low (felv) or increased (rd-114 virus) levels of fibrinolytic activity and do ... | 1975 | 1080752 |
| igm antibody-dependent cell-mediated cytotoxicity in the moloney sarcoma virus system: the involvement of t and b lymphocytes as effector cells. | antibody-dependent cell-mediated cytotoxicity in the moloney sarcoma virus (msv) system was analyzed with respect to the subpopulations of effector cells involved in tumor target cell destruction when igm was used as the sensitizing antibody. with unfractionated sera from animals that had undergone regression primary msv tumors it was found that macrophages did not contribute to the cytotoxicity induced by normal spleen cells that were syngeneic to the target cells. the igm fraction of msv regre ... | 1975 | 1080777 |
| detection of cytotoxic lymphoid spleen cells from stu-mice with moloney sarcoma by a 3h-proline microcytotoxicity assay. | a microcytoxicity assay with 3h-proline prelabeled target cells was used for the detection of sensitized lymphoid spleen cells from stu inbred mice inoculated with moloney sarcoma virus (msv-m) or ascitic msv-m tumor cells. the target cell line was derived from ascitic msv-m tumor cells. with regard to the specificity of the assay nonimmune slpeen cells displayed no or only a weak cytotoxicity against these cells, and this was also the case when 3h-proline-labeled secondary cultures of syngeneic ... | 1976 | 1084460 |
| two functionally distinct anti-tumor effector cells isolated from primary murine sarcoma virus-induced tumors. | the ability of cells from primary msv-induced tumors to function as effector cells in vitro was evaluated. host cells were isolated by enzymatic disaggregation of the tumor and fractionated by sedimentation velocity at unit gravity on a ficoll gradient. characterization of these cells indicated that 30 to 40 % were t lymphocytes, about 50% were macrophages and less than 5% were b lymphocytes. two different functional activities were mediated by these cells: cytolysis, as measured by the cra, and ... | 1976 | 1084897 |
| leukemic mitochondria. ii. acute monoblastic leukemia. | qualitative and quantitative ultrastructural studies were performed on mitochondria of leukemic monoblasts from 15 patients with acute monoblastic leukemia. similar comparative observations were made on mitochondria of myeloblasts from 14 hematologically normal controls. no significant quantitative differences were noted between normal and leukemic mitochondria. area measurements were approximately equal. qualitative differences between the two groups consisted of increased numbers of irregularl ... | 1976 | 1062229 |
| production of tumor-specific inducer of cellular cytotoxicity by lethally irradiated mice. | antisera taken 1 or 2 days after inoculation of balb/c mice with transplantable sarcoma cells or moloney sarcoma virus (msv) induce tumor-specific cell-dependent cytotoxicity in vitro. in the present experiments, lethally irradiated ("immunosuppressed") mice were tested for the early appearance of the serum factor responsible for this anti-serum-dependent cell-mediated cytotoxicity (e-adc). balb/c mice were infected with msv, syngeneic sarcoma cells or sheep red blood cells 24 h following irradi ... | 1976 | 955742 |
| immunogenic properties of a nonproducer malignant tumor induced by murine sarcoma virus. | a cloned cell line h-11 (hp) derived from an msv-induced neoplasm (a hemangiosarcoma) was found to possess virus-specific tumor rejection antigen(s). the specific nature of the immune response was established through the use of a polyoma virus-induced neoplasm no. 89 both in cross immunization and cross challenge experiments. virus antigens or surface antigens associated with virus production are not responsible for tumor rejection since virus production could not be detected, nor were any viral ... | 1976 | 967890 |
| suppression of moloney sarcoma virus immunity following sensitization with attenuated virus. | murine sarcoma virus (moloney strain) (msv-m)-induced tumors are unusual in that they regularly appear less than 2 weeks after virus inoculation, progress for 1 to 2 weeks, and are rejected by normal adult balb/c mice. rejectio leaves the animals immune to tumor induction. in the present study, presensitization of normal adult balb/c mice with attenuated msv-m resulted in an altered pattern of tumor immunity. injection of active msv-m into the presensitized animals resulted in tumor induction an ... | 1976 | 1000500 |
| biological properties of cell lines derived from moloney virus-induced sarcoma. | two cell lines derived from a primary msv-m-induced tumor in a balb/c mouse were studied. one line (ms-2) was subject only to continuous tissue culture transfer (tct). after 21 tct, ms-2 cells produced progressive tumors (ms-2 tumors) in syngeneic hosts. the second cell line (ms-2t) was established by cultivation of a ms-2 tumor. the ability to produce progressive tumors decreased with increased number of tct, in both cell lines. the virus content of ms-2 and ms-2t cells was very low, as shown b ... | 1976 | 1020048 |
| mixed lymphocyte/tumor-cell interaction in a murine sarcoma virus (moloney)-induced tumor system. comparison between lymphoproliferation and lymphocyte cytotoxicity. | studies of the mlti were performed in a primary msv-induced tumor system. reactivity against mitomycin-c-treated rbl-5 cells could be detected during a limited period at about 14 days after virus injection. the degree of reactivity of msv 14 spleen cells was quite variable; stimulation indices ranged from 2.2 to 9.4. activity in the mlti appeared to a great extent to be dependent on t-lymphocytes. a relatively broad spectrum of specificity was demonstrated in experiments using additional stimula ... | 1976 | 1254360 |
| antibody-dependent, cell-mediated cytotoxicity with autochthonous lymphocytes and sera after infection with moloney sarcoma virus. | antibody-dependent, cell-mediated cytotoxicity in the moloney sarcoma virus (msv) system was analyzed in terms of the ability of autochthonous antibody to induce or potentiate cytotoxicity by lymphocytes from animals infected with msv. as previously demonstrated in microcytotoxicity assays, the lymphocytes from regressor animals taken 30 days after virus infection were consistently more cytotoxic than those from tumor-bearing animals 15 days after infection. antisera from the regressors potentia ... | 1976 | 1255765 |
| histological and histochemical studies of myocardial lesions in balbc/cr mice. | myocardial lesions were noticed in balbc/cr mice during experiments with moloney sarcoma virus (msv). a study was initiated using unifected balbc/cr mice to determine whether these lesions were the result of a viral infection. sixty percent of the females and 30% of the males used were found to have macroscopic lesions (mainly white spots on the right ventricle). the reported lesions were studied both histologically and histochemically. the animals were also examined for parasites such as toxopl ... | 1976 | 1271745 |
| nucleotide sequences in mouse dna and rna specific for moloney sarcoma virus. | complementary dna (cdna) synthesized by moloney murine sarcoma virus (m-msv) was separated into two parts, the first, termed msv-specific cdna, composed of nucleotide sequences found only in m-msv viral rna, and the second, termed msv-mulv common cdna, composed of nucleotide sequences that were found in both m-msv and murine leukemia virus (mulv) viral rnas. rna complementary to the msv-specific cdna was not found in several other msv isolates, nor in ecotropic mulv, mouse mammary tumor virus, o ... | 1976 | 185623 |
| role of interferon in the pathogenesis of virus diseases in mice as demonstrated by the use of anti-interferon serum. ii. studies with herpes simplex, moloney sarcoma, vesicular stomatitis, newcastle disease, and influenza viruses. | the effect of potent sheep anti-mouse interferon globulin was investigated in several different experimental virus diseases of mice. in anti-interferon globulin-treated mice infected intraperitoneally with herpes simplex virus (hsv) type i, the latent period was shortened, and the overall ld50 was increased several hundredfold compared to virus-infected control mice. when hsv was inoculated subcutaneously all anti-interferon globulin-treated mice died, whereas only 5% of virus-infected control m ... | 1976 | 186555 |
| antigenic specificity of the cytolytic t lymphocyte (ctl) response to murine sarcoma virus-induced tumors. i. preferential reactivity of in vitro generated secondary ctl with syngeneic tumor cells. | incubation of spleen cells from mice having rejected a moloney sarcoma virus (msv)-induced tumor with syngeneic irradiated lymphoma or sarcoma cells bearing msv-associated antigens in secondary mixed leukocyte-tumor cell cultures (mltc) resulted in the generation of highly active cytolytic t lymphocytes (ctl) specifically directed against syngeneic target cells bearing msv-associated antigens. when msv-immune spleen cells from c57bl/6 (h-2b) and balb/c(h-2d) mice were compared with respect to th ... | 1976 | 187429 |
| the 30s moloney sarcoma virus rna contains leukemia virus nucleotide sequences. | the 50s-70s rna of a moloney sarcoma-leukemia virus [mo-msv(mlv)] complex produced by a particular mouse cell line was shown by gel electrophoresis to contain a major (97%) 30s sarcoma-specific subunit species and a minor (3%) 38s leukemia virus-specific subunit. on the basis of its sedimentation coefficient and known complexity, the 30s mo-msv rna was estimated to be a unique rna molecule of about 6000 nucleotides. hybridization experiments using viral rna and dna complementary to viral rna (cd ... | 1976 | 184965 |
| recent studies on the identification of proliferative abnormalities and of oncogenic potential of cutaneous cells in individuals at increased risk of colon cancer. | this recent study describes the growth characteristics of acr skin fibroblasts in culture and their differential susceptibility to transformation by kirsten murine sarcoma virus (ki-msv). the sf were derived from normal appearing subepidermoid biopsies of acr individuals, their progeny and ocntrols. normal sf were contact-inhibited and grew only in 15% fcs. sf of acr subjects, and some asymptomatic acr progeny were not contact inhibited, grew in both 1% and 15% fcs and were considerably more sus ... | 1976 | 11561 |
| morphological, biological, immunological and biochemical studies on bone tumors of animals and man. | biological studies on fbj osteosarcoma virus in tissue cultures have led to the isolation of murine sarcoma virus. characteristic type c-mulv particles were observed in bone tumors induced by the sd-msv-m-virus in vitro and in vivo. the sd-msv-m virus also induced bone tumors in rats of all strains tested, and it has a similar tumor-inducing property in hamsters. immunoelectronmicroscopic studies showed that envelope antigens of msv-sd virus in rat bone tumors can be distinguished from those fou ... | 1976 | 189370 |
| a comparison of membrane glycoconjugates from mouse cells transformed by murine and primate rna sarcoma viruses. | kirsten murine sarcoma virus (ki-msv) transformed balb/et3 mouse cells (k-balb) were found to have altered membrane glycoconjugates compared to normal balb/3t3 cells. there were reduced amounts of mono- and disialogangliosides, gm1 and gd1a, and activity of the specific galactosyltransferase required for synthesis of these gangliosides was reduced to between 0 and 18.5% of normal in the several k-balb clones examined. when fucose-labeled glycopeptides derived from the surfaces of balb/3t3 and k- ... | 1976 | 174712 |
| altered microfilament structure in cells transformed with a temperature-sensitive transformation mutant of murine sarcoma virus. | the structure and distribution of microfilaments were examined by electron microscopy in uninfected normal rat kidney (nrk) cells, murine sarcoma virus (msv)-transformed nrk cells, and nrk cells infected with a cold-sensitive transformation mutant of msv, i.e., nrk (msv-1b) cells, grown at both permissive (39 degrees) and nonpermissive (33 degrees) temperature. the uninfected cells contained numerous microfilaments which were especially prominent at sites of intercellular adherens junctions. in ... | 1976 | 174810 |
| attempts to induce tubular deformations and subsequent mixed tumours from organotypic cultures of mouse renal mesenchyma. | with the aim of expanding knowledge on the pathogenesis of nephroblastomata, an embryological organ culture system (grobstein, 1956) was tested for its applicability to in vitro carcinogenesis experiments by using murine sarcoma virus (msv-m) and 3-methylcholanthrene (mca). treatment of cba/h-t6 mouse metanephrogenic mesenchyma with msv-m at the pretubular stage neither disturbed the glomerulogenesis nor induced rapid malignant transformation. treatment of the same tissues with mca considerably ... | 1976 | 175592 |
| fractionation of dna nucleotide transcripts from moloney sarcoma virus and isolation of sarcoma virus-specific complementary dna. | radioactive dna complementary to nucleotide sequences in moloney murine sarcoma virus (msv) and moloney leukemia virus (m-mulv) complex was made by the endogenous reverse transcriptase reaction. these virus stocks contained a threefold excess of msv over m-mulv as measured by biological assay. the complementary dna was an accurate copy of the viral rna in that 86% of 35s viral rna hybridized with complementary (cdna) dna at a 1.5 to 1 cdna-rna molar ratio. the complementary dna, of a 4-6s size, ... | 1976 | 178890 |
| suppression of mixed lymphocyte response in mice bearing primary tumors induced by murine sarcoma virus. | mixed lymphocyte culture (mlc) responses of spleen cells from mice bearing tumors induced by murine sarcoma virus of the moloney type (msv) against allogeneic spleen cells were found to be markedly depressed, as estimated by three parameters: lymphoblast counts, 3h-thymidine incorporation (ti), and cell-mediated lysis (cml). while all three parameters were definitely depressed in comparison to the normal controls, ti was depressed to a greater degree than lymphoblast counts and cml. msv spleen c ... | 1976 | 179171 |
| characterization of effector lymphocytes associated with immunity to murine sarcoma virus (msv) induced tumors. i. physical properties of cytolytic t lymphocytes generated in vitro and of their immediate progenitors. | cytolytic t lymphocytes (ctl) were generated in secondary mixed leukocyte-tumor cell cultures (mltc) with syngeneic rb1-5 tumor cells as stimulating cells and with responding spleen cells from regressor mice that had rejected a murine sarcoma virus (msv)-induced tumor. ctl precursor cells were found to be exclusively of thymic origin and non-t cells were apparently not required for ctl generation. when the size variations of ctl from syngeneic mltc were analyzed by velocity sedimentation it appe ... | 1976 | 180182 |
| inhibition of murine sarcoma virus induced transformation by adenovirus structural proteins. | the purified fibre and hexon of adenovirus 12 inhibit the transformation in tissue culture of murine sarcoma virus (msv-m) by as much as 80% and 70%, respectively, when they are added to cells 8 to 2o h before msv-m infection. during a 12 h period, only about 6 to 8% of added radiolabelled viral proteins become associated with cells (or i-o mug protein bound/10(5) cells). no inhibition occurs when the proteins are added simultaneously with msv-m or 90 min or 4 h after msv-m. there is also a dire ... | 1976 | 180250 |
| phenotypic mixing between n- and b-tropic murine leukemia viruses: infectious particles with dual sensitivity to fv-1 restriction. | in effort to understand how n or b tropism is determined in murine leukemia virus (mulv) particles, we analyzed the mulv produced after dual infection of mouse cells by n- and b-tropic mulv. the progeny mulv from such a mixed infection are sensitive to fv-1 restriction in both n- and b-type cells, but are still highly infectious for mouse cells which do not exhibit fv-1 restriction. this dual sensitivity to fv-1 restriction is a phenotypic property of mulv produced by mixedly infected cells, sin ... | 1976 | 181144 |
| cell-mediated immunity to leukemia virus- and tumor-associated antigens in mice. | cell-mediated immune reactions appear to play an important role in resistance against growth of leukemia cells in mice. possible mechanisms for in vivo protection in two tumor systems are discussed. these tumor models, which are a friend leukemia virus-induced transplantable tumor, fbl-3, and primary murine sarcoma virus (msv) -induced tumors, are strongly antigenic; under some conditions, tumors regress completely. in mice with regressing fbl-3 tumors, cell-mediated cytotoxicity was measured by ... | 1976 | 56223 |
| induction of a novel transforming virus (msv-z) from sarcoma-positive leukemia-negative 3t3fl mouse cells. | 1976 | 56806 | |
| sequence homology between moloney murine sarcoma virus and moloney leukemia virus rna. | the moloney murine sarcoma-leukemia virus [m-msv (mulv)], propagated at high multiplicity of infection (moi), was demonstrated previously to contain a native genome mass of 4 x 10(6) daltons as contrasted to a mass of 7 x 10(6) daltons for moloney murine leukemia virus (m-mulv). the 4 x 10(6)-dalton classof rna from m-msv (mulv) was examined for base sequence homology with dna complementary to the 7 x 10(6)-dalton m-mulv rna genome. approximately 86% of the m-msv (mulv) was protected from rnase ... | 1976 | 59817 |
| immunological properties of murine thymus-dependent lymphocyte surface glycoproteins. | the immunological properties of two murine thymus-dependent (t) lymphocyte surface glycoproteins, t200 and t25, were investigated. t200 is a lymphocyte-specific antigen with a high degree of species specificity. it shares antigenic determinants with molecules present on thymus-independent (b) lymphocytes. t25 has antigenic determinants which cross-react with antigens on mouse brain, rat thymocytes and rat brain. an antiserum against a purified rat brain glycoprotein which carries thy-1.1 reacts ... | 1976 | 61878 |
| cells transformed by certain strains of moloney sarcoma virus contain murine p60. | it was previously demonstrated that the 60,000 dalton (p60) precursor-like polyprotein containing murine p30 was a constituent of the feline leukemia virus pseudotype of moloney sarcoma virus [m1msv(felv)]. it is now shown that p60 is detected in cells of five mammalian species transformed by m1msv, indicating that p60 is specified by this genome. moreover, little or no murine p30 is detected in the m1msv-transformed cells, suggesting that the murine group p30 antigenic reactivity of s + l- cel ... | 1977 | 65230 |
| genetics of murine sarcoma virus (msv)--induced tumors in akr mice: evidence that late progressing and early regressing tumors are controlled by different gents. | the genetics of late appearing msv tumors showing a progressive growth pattern in akr mice was investigated. the late msv tumor response in f1 hybrids depended on the genetic background of the non-akr parent. within the 4-month observation period following virus injection, (cba x akr) f1, (dba/2 x akr)f1, and (nih x akr)f1 developed progressing msv tumors, which exhibited latency and growth behavior comparable to that seen in akr mice, (balb x akr)f1, (b6 x akr)f1, and (b10br x akr)f1 mice did n ... | 1977 | 66212 |
| search for oncogenic viruses in human prostate cancer. | electron microscopic, immunologic, and biochemical methods have been used in an attempt to detect and characterize oncornaviruses in human prostatic carcinoma (pca) and benign prostatic hyperplasia (bph), and in prostates of mice of high and low mammary cancer or leukemia strains. ultrastructural examination of 37 pca and nine bph specimens has revealed the presence of particles resembling type c virus in five cases of pca and one of bph, and also two different types of intracisternal virus-like ... | 1977 | 68817 |
| phosphorylation and nucleic acid binding properties of m1 moloney murine sarcoma virus-specific pp60gag. | the pp60gag polyprotein of the feline leukemia virus pseudotype of m1 moloney murine sarcoma virus [m1msv(felv)] was previously shown to be msv specific and to contain murine p30 and smaller structural polypeptides. this protein was detected in m1msv-transformed cells, and in pulse-chase studies it was found to be stable. in this study virion p60 was shown to contain murine pp12, to be phosphorylated, and to bind to nucleic acids. 32p-labeled m1msv[felv) was fractionated by guanidine agarose chr ... | 1977 | 69721 |
| virus-induced animal model of osteosarcoma in the rat: morphologic and biochemical studies. | osteosarcomas were produced by the intratibial inoculation of new zealand black rats with moloney sarcoma virus (msv) at 1 day and 4 days of age. radiographic evidence of osteosarcoma development was first demonstrated at 10 to 15 days postinoculation in both groups. subsequent radiographic and light and electron microscopic evaluation of tumor-bearing rats demonstrated that osteosarcomas in rats inoculated at day 4 of age were more osteoproliferative osteosarcomas than those in rats inoculated ... | 1977 | 189616 |
| effect of pretreatment with immune serum on murine sarcoma virus (moloney) tumour induction and growth. | regressor serum from msv-m-infected mice markedly reduced msv-m oncogenesis when administered i.p. (0-1 ml/mouse) as much as 30 days before i.m. msv-m infection in adult balb/c mice. the regressor serum activity appeared to be directly dependent on the amount of igg, as shown by: (1) inactivity of sera which have low virus-neutralizing antibody content; (2) high effectiveness only of the igg serum fraction; (3) inactivity of regressor serum incubated with anti-mouse gamma-globulin serum. the reg ... | 1977 | 189792 |
| loss of fv-1 restriction in balb/3t3 cells following infection with a single n tropic murine leukemia virus particle. | the ability of various murine leukemia viruses (mulvs) to replicate in mouse cells exhibiting fv-1 restriction was analyzed by quantitative dose-response assays. in particular, the effect of infection with n, b, or nb tropic mulvs on fv-1b restriction in balb/3t3 cells was measured with an infection center technique in which pseudotypes of murine sarcoma virus (msv), which have been shown to exhibit fv-1 dependence of expression, were used to quantitate the degree of restriction. the resulting d ... | 1977 | 191197 |
| stimulation of mouse migration inhibitory factor (mif) production form msv-immune lymphocytes by soluble tumor-associated antigen: requirement for histocompatible macrophages. | spleen cells from c57bl/6 mice immunized with murine sarcoma virus (msv) are capable of producing migration inhibition factor (mif) in response to stimulation with a specific tumor-associated antigen prepared by solubilization with 3 m kcl. we have previously demonstrated that this response is t cell-dependent. further investigations into the effector cells involved in the production of mif have revealed that spleen cells from mice immunized with msv cannot produce mif when stimulated with tumor ... | 1977 | 191532 |
| murine sarcoma virus pseudotypes used as immunogens against viral and chemical oncogenesis. | the purpose of this study was to develop an animal system of protective immunity against oncornaviruses and to test whether such immunization had an inhibitory effect upon chemical sarcomagenesis. several murine sarcoma virus (msv) pseudotypes were used as immunogens and tested against themselves, against other pseudotypes, against leukemogenesis by their helper viruses, and against sarcomagenesis by 3-methylcholanthrene. five msv pseudotypes were obtained by rescuing complete msv from msv-genom ... | 1977 | 192461 |
| relationships between h-2 and viral antigens in murine oncornavirus-induced tumours. | cytolytic t lymphocytes (ctl) from murine sarcoma virus (msv) or friend leukaemia virus (flv) inoculated mice lyse syngeneic much more efficiently than allogeneic fmrgi+ lymphoma cells. by comparing the cytolysis of various h-2 different 51cr lymphomas by ctl from several inbred and congenic lines differing at h-2, and by competition experiments using unlabelled cells, one can demonstrate that this phenomenon is due to an h-2 barrier. h-2b/h-2d hybrid-anti-msv-ctl immunized by h-2b, h-2d or h-2b ... | 1977 | 192802 |
| characterization to effector lymphocytes associated with immunity to murine sarcoma virus (msv)-induced tumors. ii. repeated stimulation and proliferation in vitro of specific cytolytic t lymphocytes. | 1977 | 196025 | |
| exclusive involvement of h-2db or h-2kd product in the interaction between t-killer lymphocytes and syngeneic h-2b or h-2d viral lymphomas. | it was demonstrated previously that the cytolysis of murine viral lymphoma cells by anti-murine sarcoma virus (msv) syngeneic t-killer lymphocytes was restricted by some products of the h-2 complex. the respective role of the products of different regions of the h-2 complex were studied with six h-2(b) and three h-2(d) lymphomas induced by five different type c viruses. they were tested in a classical chromium release test against anti-msv t-killer cells obtained from different inbred strains of ... | 1977 | 197198 |
| effect of helper virus on the number of murine sarcoma virus dna copies in infected mammalian cells. | cell lines of four mammalian species were each examined for the number of moloney murine sarcoma virus (m-msv) dna copies in total cellular dna after m-msv transformation. sarcoma-positive, leukemia-negative (s+l-) m-msv-transformed cells were compared to m-msv-transformed cells infected with a replicating leukemia virus. both unfractionated m-msv complementary dna and complementary dna representing the msv-specific and the msv-murine leukemia virus-common regions of the m-msv genome were hybrid ... | 1977 | 197257 |
| relationship between moloney murine leukemia and sarcoma virus rnas: purification and hybridization map of complementary dnas from defined regions of moloney murine sarcoma virus 124. | complementary dnas (cdna's) specific for various regions of the moloney murine sarcoma virus (msv) 124 rna genome were prepared by cross-hybridization techniques. a cdna specific for the first 1,000 nucleotides adjacent to the rna 3' end (cdna 3') was prepared and shown to also be complementary to the 3'-terminal 1,000 nucleotides of a related moloney murine leukemia virus (mlv) genome. a cdna complementary to the "msv-specific" portion of the msv 124 genome was prepared. this cdna was shown not ... | 1977 | 197259 |
| cyclic amp-induced morphological transformation of cells infected by temperature-sensitive mouse sarcoma virus. expression of transformation-associated markers. | normal rat kidney (nrk) cells infected with a temperature-sensitive (ts) mutant of mouse sarcoma virus (nrk [msv-1b]) express the transformed phenotype when grown under permissive conditions, but acquire the normal phenotype when grown under restrictive conditions. addition of 3', 5' cyclic adenosine monophosphate (camp) to nrk (msv-1b) cells grown at the restrictive temperature results in morphological transformation. to determine whether other markers associated with the transformed phenotype ... | 1977 | 198411 |
| characterization of gazdar murine sarcoma virus by nucleic acid hybridization and analysis of viral expression in cells. | gazdar murine sarcoma virus (gz-msv) and moloney murine sarcoma virus (m-msv) are closely related. the complete m-msv-specific nucleic acid sequences constituted a major portion of gz-msv-specific sequences. the msv-specific sequences in both gz-msv and m-msv genomes shared homology with hamster leukemia virus nucleic acid sequences. both rat cells (s+l+) and hamster (s+l-) cells expressed two viral proteins of 68,000 and 70,000 daltons. these proteins were immunologically related to p60 purifie ... | 1977 | 199741 |
| replication of mouse sarcoma virus (moloney) and its helper in a human heteroploid cell line of malignant origin. | a human heteroploid cell line of malignant origin (j111) was infected with mouse moloney sarcoma virus. [msv-m(mlv)]. the replication of the virus was demonstrated by different methods. after a few passages of infected cultures, the transforming component of msv-m(mlv) disappeared from the supernatant fluids and cells. the non transforming helper component continued to be produced. the relatively low amount of helper virus released by the cells suggests a control of its replication in the j111 c ... | 1977 | 206868 |
| detection of human c-type "helper" viruses in human leukemic bone marrow with murine sarcoma virus-transformed human and rat non-producer cells. | bone-marrow cells from two leukemic children were co-cultivated with the leukemic children a 7573. in early passages, c-type oncornaviruses were released as detected by extracellular reverse transcriptase assay. co-cultivation of the infected canine cells with the non-producing cell lines r-970-5 (human) or k-nrk (rat) both transformed by kirsten mouse sarcoma virus (msv) yielded a new pseudotype of msv that could transform rat embryo, rabbit sirc and human kidney cells but not mouse embryo cell ... | 1977 | 188771 |
| transformation of cultured rat adrenocortical cells by kirsten murine sarcoma virus (ki-msv). | two-week-old primary cultures of normal adult rat adrenal cortex were exposed to kirsten murine sarcoma virus (kimsv). within a week, the adrenal cells, which are normally fusiform and aligned in parallel, became pleomorphic and piled up extensively. saturation density increased from 5-10 x 10(4) to 5-10 x 10(5) cells/cm2, population doubling time during exponential growth decreased from 36-40 to 16h, acid production increased and the growth rate became independent of a reduction in serum concen ... | 1977 | 188772 |
| rate of divergence of cellular sequences homologous to segments of moloney sarcoma virus. | the rna genome of the moloney isolate of murine sarcoma virus (m-msv) consists of two parts--a sarcoma-specific region with no homology to known leukemia viral rnas, and a shared region present also in moloney murine leukemia virus rna. complementary dna was isolated which was specific for each part of the m-msv genome. the dna of a number of mammalian species was examined for the presence of nucleotide sequences homologous with the two m-msv regions. both sets of viral sequences had homologous ... | 1977 | 189062 |
| [functional activity of cytolytic t lymphocytes in the rejection of tumor target cells]. | sensitized t lymphocytes can specifically destroy in vivo target cells with which they enter into direct contact. this suggests an important role for cytolytic t lymphocytes (ctl) in the destruction of grafts and tumours. quantitative methods allow the assay of ctl and of ctl memory cells which appear in lymphoid tissues, blood, grafts and tumours during rejection. the formation of ctl and ctl memory cells can also be induced in vitro, and the functional activity of such cells has been demonstra ... | 1977 | 139840 |
| interruption of oncornavirus replication by modified rifamycin antibiotics. | thirteen rifamycin sv derivatives containing 3'-alkylaminomethyl substituents fail to inhibit the activities of the simian sarcoma virus type 1 dna polymerase, and of cellular dna, rna, and poly(a) polymerases prepared from nih swiss mouse embryos. these compounds show a range in their toxicities for nih swiss mouse 3t3 cells and in their capacities to inhibit production of foci of morphologically altered cells by murine sarcoma virus (msv). three compounds--the n-methyl-n-hydroxyethylaminomethy ... | 1977 | 81643 |
| antibody-dependent cell-mediated cytotoxicity in the moloney sarcoma virus system: differential activity of igg and igm with different subpopulations of lymphocytes. | antibody-dependent cell-mediated cytotoxicity in the moloney sarcoma virus (msv) system was evaluated in terms of the differential ability of igg and igm from msv regressor animals to induce cytotoxicity by lymphocytes from lymph node, spleen, and thymus. the cell-mediated cytotoxicity induced by both igm and igg was specific for target possessing the appropriate virally determined cell surface antigen(s). igm induced cytotoxicity by lymphocytes from all the organs tested. however, differences i ... | 1977 | 833543 |
| a heteroduplex study of the sequence relationships between the rnas of m-msv and m-mlv. | the regions of sequence homology between the rna genomes of a murine sarcoma virus (clone 124 moloney-msv) and its parental helper virus (clone 3 moloney-murine leukemia virus (m-mlv)) have been mapped. long complementary dna transcripts of the m-mlv rna were hybridized to m-msv rna, and the structures of the hybrids were observed in the electron microscope. beginning at the 5' end, the two rnas are homologous for a region of length 2.25 kb (kilobases). in the next region, of length approximatel ... | 1977 | 844103 |
| defective friend spleen focus-forming virus: interfering properties and isolation free from standard leukemia-inducing helper virus. | defective friend spleen focus-forming virus (sffv) is able to interfere with the ability of its naturally occurring leukemia-inducing helper virus (llv-f) to induce xc plaque formation in several different strains of mouse embryo cells. this interference has been observed by using two different sffv preparations, one contained in an nb-tropic stock of friend virus (fv) complex, and the second present in a c57bl-adapted strain of fv complex containing an associated b-tropic llv-f helper. the llv- ... | 1977 | 904028 |
| alterations in the mitogen responsiveness of lymphocytes from mice bearing moloney sarcoma virus induced tumors. | the effects of moloney sarcoma virus (msv) induced tumor growth dynamics on the blastogenic responsiveness of lymphocytes from balb/c mice were investigated. lymphocytes from spleen, thymus and lymph node pools were tested for responsiveness to phytohemagglutinin (pha) and concanavalin a (con a). results showed a significant decrease in pha-induced blastogenesis of all lymphocytes tested at the time of maximal tumor volume, with a return to normal responsiveness as the tumor regressed. in contra ... | 1977 | 304835 |
| an animal model for human osteosarcoma. | osteosarcomas formed in antilymphocyte serum (als)-treated hamsters when 2x10(6) te-85 human osteosarcoma cells (maintained in tissue culture) infected with m-msv (rd-114) virus were injected adjacent to the femur or the scapula; undifferentiated sarcomas formed when 1 x 10(6) cells were injected subcutaneously. tumors were palpable 10 to 14 days after the cells were injected and grew progressively until the animals died (mean survival time was 30 days). all animals had pulmonary metastases. nei ... | 1977 | 264708 |
| characterization of human cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine. | human osteosarcoma (hos) clonal cells transformed in vitro by n-methyl-n'-nitro-n-nitrosoguanidine (mnng) were characterized, and compared to non-producer hos cells transformed by kirsten murine sarcoma virus (ki-msv). the mnng- and virus-transformed cells grew in the aggregate form above an agar base, grew in soft agar, and had a high fibrinolytic activity. when inoculated into nude mice, all the chemically or virally altered cells produced tumors or tumor nodules. when transplanted into ats-tr ... | 1977 | 265298 |
| pathology of neoplasms and other lesions induced in rats with moloney murine sarcoma virus. | moloney murine sarcoma virus (m-msv) was injected directly into the fetuses of sprague-dawley rats during the late stage of gestation and into the neonates within 24 hours after birth. ninety rats developed 188 neoplastic lesions during the 8-week period of observation. nearly all of the neoplasms were of mesenchymal derivation. sixty percent of these neoplasms revealed more complex histologic features than those previously reported for neoplasms induced in rodents with m-msv and were designated ... | 1977 | 266356 |
| xeroradiographic evaluation of murine osteosarcoma. | xeroradiography, a method of x-ray imaging based upon selenium photoconductivity, was used for the study of experimental osteosarcoma induced by msv-m virus in rats. due to the peculiar features of xeroradiographic image (enhancement of details and lowering of the overall contrast) good pictures of osseous structures together with soft tissues were obtained even in very young animals. serially perfomred xeroradiographies gave a permanent representation of tumor evolution with time. advantages an ... | 1977 | 270862 |
| murine sarcoma virus (msv)-induced tumors in mice. i. distribution of msv-immune cytolytic t lymphocytes in vivo. | quantitative studies using a 51cr release assay were performed to analyse the time-course of appearance and specificity of cytolytic cells in c57bl/6 mice inoculated with moloney murine sarcoma virus (msv). various lymphoid organs were studied including spleen, mesenteric lymph nodes (mln), lymph nodes regional to the msv-induced tumor (rln), and peripheral blood. furthermore, the kinetics of appearance of cytolytic cells within the msv-induced tumor was determined. in agreement with previous st ... | 1977 | 300075 |
| t and b lymphocyte migration into syngeneic tumors. | the migration of splenic t and b lymphocytes into syngeneic tumors undergoing immunologic rejection was investigates. spleen cells were obtained from normal balc/c mice or balb/c mice bearing tumors induced by murine sarcoma virus (msv). either whole spleen cells or immunoabsorbent purified t and b cells were radiolabeled with sodium chromate-51 and injected i.v. into normal or msv inducted-tumor bearing syngeneic recipients. twenty-four hours later the recipient mice were sacrificed and radioac ... | 1977 | 300384 |
| activation of k cells in mice with transplanted tumours differing in immunogenicity and metastasizing capacity. | the effector arm of antibody-dependent cellular cytotoxicity (adcc) was evaluated using 51cr-labelled chicken erythrocytes as targets in balb/c mice transplanted with the moloney sarcoma virus-induced tumours t-msv and ms2, and in c57bl/6 mice transplanted with the chemically induced fs6 sarcoma, lewis lung carcinoma and b16 melanoma. tumour-bearing animals showed higher levels of adcc than normal mice, a stimulation confirmed in ms2-bearing mice, using sl2 lymphoma cells as targets in a cytosta ... | 1977 | 588412 |
| protective effect of murine sarcoma virus-superinfected mouse tumor cells against outgrowth of corresponding noninfected tumor. | cultured lines of three newly established methylcholanthrene-induced tumors, mbk and mbl in cba and mc57m in c57bl mice, and two mammary tumors, sbfnhc in cba and s3w in asw mice, were superinfected in vitro with moloney sarcoma or leukemia virus (msv, mlv). after superinfection, they expressed the moloney virus-determined cell surface antigen (mcsa) and murine c-type viral p30 antigen, and produced nb-tropic c-type virus. the virus-infected tumors became more rejectable in normally susceptible ... | 1978 | 705875 |
| effect of non-steroidal anti-inflammatory drugs of moloney sarcoma virus inoculated mice. | chronic administration of phenylbutazone, flufenamic acid and a new, potent non-steroidal anti-inflammatory agent itf (3-methyl-5-benzoyl-amino-isothiazole-4-carboxy-p-ethoxyphenylamine) to balb/c mice inoculated with a moloney sarcoma virus resulted in a stimulation of tumor growth and increased severity of disease. this treatment, however, had no effect on the spontaneous regression of tumors. indomethacin in a dose of 5.0 or 2.5 mg per kg suppressed the msv-induced tumor growth, but this effe ... | 1978 | 729732 |
| epithelioid and fibroblastic rat kidney cell clones: epidermal growth factor (egf) receptors and the effect of mouse sarcoma virus transformation. | fibroblastic and epithelioid clones have been isolated from the normal rat kidney line, nrk. these clones were studied for their ability to bind epidermal growth factor (egf), susceptibility to transformation by mouse sarcoma virus (msv), and alteration in egf binding upon sarcoma virus transformation. the epithelioid clones bound much more egf than the fibroblastic clones; scatchard plots on two of these clones, one epithelioid and one fibroblastic, showed that the higher egf binding (1.3 x 10( ... | 1978 | 304450 |
| [transplantable experimental tumor. morphological and ultrastructural study (author's transl]. | an experimental tumor which has been induced in newborn chicken, was transplanted into inbred balb/c mice and then studied by the means of light and electron microscopy. sendai virus fusion between msv-h transformed rat cells and chickembryo cells provided the tumor. histological pattern of this neoplasia seems to be superposable, both in chicken and in mouse, to that of anaplastic fibrosarcomas and shows some differences from either rous sarcoma or balb/c mouse h-6668 spontaneous rabdomyosarcom ... | 1978 | 224888 |
| use of trypsin for rapid and efficient purification of murine sarcoma and leukemia virus. | a method is described for purification of msv-mulv from culture supernatant of chronically infected 78a,1 rat embryo cell line. this method involves direct polyethylene glycol-nacl precipitation of the low speed supernatant of culture fluid followed by digestion of the pellet with trypsin. this procedure efficiently disrupts large aggregates which normally entrap most of the virus. highly purified virus can be obtained in very good yield by a combination of sedimentation velocity and isopycnic c ... | 1978 | 88963 |
| transformation of indian muntjac cells by murine and avian sarcoma viruses. | indian muntjac cells were efficiently transformed by murine sarcoma virus (msv) and avian sarcoma viruses (asv). when colony formation of the infected cells was examined in soft agar, many colonies were formed by the asv-injected cells but no colony was seen in the msv-infected cells. the asv-transformed cell clones differed among the clones in morphology, presence of inducible asv genome, and karyotypes. | 1978 | 208907 |
| effect of interferon on murine leukemia virus infection. iii. effect of interferon on msv-induced transformation. | 1978 | 213891 | |
| [effect of sodium butyrate on the interferon sensitivity of normal or cells transformed by murine sarcoma virus]. | sodium butyrate enhances the sensitivity of msv transformed murine cells to interferon, but does not influence the response of normal cells. sodium butyrate might act in transformed cells by restoring the different elements of the cytoskeletal system. | 1978 | 214252 |
| chemical determination of the m1 moloney sarcoma virus pp60gag gene order: evidence for unique peptides in the carboxy terminus of the polyprotein. | the gene order of the ml moloney sarcoma virus (mlmsv) specific pp60gag (p60) was determined by direct chemical analysis of the polyprotein. p60 was cleaved with cyanogen bromide (cnbr) into eight partial and complete fragments ranging in mass from 10,000 daltons to 58,000 daltons. peptide maps of these fragments were compared to maps of p15, p12, and three cnbr fragments of p30. the polarity of p15 and p12 in a cnbr fragment of p60 was determined by carboxypeptidase a digestion; likewise the cn ... | 1978 | 216995 |
| revertants of mouse cells transformed by murine sarcoma virus. v. loss of msv-specific nucleotide sequences from cellular rna. | 1978 | 217157 | |
| application of antisera to interferon in studying oncogenic viruses. | susceptibility of balb/c mice to infection with moloney sarcoma virus (msv) and to herpes simplex virus type 2 (hsv-2) was considerably increased by administration of sheep anti-mouse interferon (anti-if) serum. the regression of the msv-induced tumors was inhibited when the weanling (three-to-four-week-old) mice were injected with the anti-if serum. using the anti-if serum it has been found that the antagonism between hsv-2 and rauscher leukemia virus in the mouse is not mediated by interferon. ... | 1978 | 219809 |
| relationship between moloney msv tumor resistance and endogenous virogene expression in akr mouse strain and its hybrids. | we have examined the induction of moloney-msv tumors in akr and other mouse strains in relation to endogenous virus expression. all virus-free strains so far tested were tumor-susceptible, while akr was resistant. the selectivity of msv tumor resistance, characteristic of akr mice, was associated with akr virogens segregation in first backcross to mulv-negative/msv-susceptible mice, and in a few second backcross families. stronger evidence that akr ecotropic virogene expression is the major, but ... | 1978 | 203548 |
| studies with the baboon endogenous virus and its pseudotype of murine sarcoma virus in marmosets. | three marmoset species (saguinus oedipus oedipus, s. fuscicollis, callithrix jacchus) failed to show evidence of infection or disease following inoculation of baboon endogenous type-c virus (baev). cells infected with a kirsten murine sarcoma-baev pseudotype (msv[baev]) produced a progressive fibrosarcoma in s. o. oedipus and tumors in s. fuscicollis and c. jacchus which regressed. tumor formation is believed to be due to growth of inoculated, virus-infected cells and not transformation of marmo ... | 1978 | 205851 |
| deposition of retrovirus associated antigens (p30 and gp70) on cell membranes of feline and murine leukaemia virus infected cells. | a quantitative estimation of retrovirus associated cell membrane antigens of murine and feline cells infected with their respective type c leukosis virus is presented. using a radio-immune assay with three broadly reactive antisera, the minimum estimated number of retrovirus associated antigenic determinants on yac [moloney leukaemia virus (mulv) infected murine] and fl-74 [feline leukaemia virus (felv) infected feline] cells was 1.3 x 10(6) and 1.6 x10(6) determinants per cell respectively. the ... | 1978 | 75945 |
| multiple rnase h activities in mammalian type c retravirus lysates. | lysates of moloney murine sarcoma-leukemia virus [m-msv(mlv)], a virus complex grown in the rat cell line 78a-1, were found to contain three rnase h species separable by polycytidylic acid[poly(c)]-agarose chromatography. rnase h activity (rnase h i) associated with rna-directed dna polymerase eluted at 0.23 m kci from poly(c)-agarose. rnase h ii, which eluted from poly(c)-agarose at 0.12 m kci and was not associated with dna polymerase activity, was shown to be identical to an rnase h species ( ... | 1978 | 77333 |
| further evidence for deletion of envelope glycoprotein (gp69/71) sequences in formation of moloney-murine sarcoma virus. | moloney-murine sarcoma virus (s+l- strain of m-msv) has been nonproductively cloned in murine and non-murine host cells (s+l- cells) and the expression of moloney leukaemia virus (m-mulv) 30000 mol. wt. core protein (p30) and envelope glycoprotein (gp69/71) were studied by radioimmunoassay. antigenic determinants of the m-mulv p30 were associated with the sarcoma virus genome in these non-productively transformed cell clones studied, while the determinants of m-mulv gp69/71 were not. the absence ... | 1978 | 80447 |
| origin and biological properties of a new balb/c mouse sarcoma virus. | a focus-forming virus previously isolated from a balb/c mouse hemangiosarcoma has been shown to be replication defective. analysis of individual balb/c mouse sarcoma virus (balb-msv) nonproducer transformants for expression of helper virus-coded proteins revealed genetically stable variants that expressed two, three, or all four gag gene products in the absence of detectable helper viral env gene expression. the type-specific antigenic determinants of helper viral proteins encoded by the balb-ms ... | 1978 | 80461 |
| cell-mediated immunity to antigens associated with murine sarcoma virus-induced tumors: augmentation of cytolytic t lymphocyte activity by successive specific and nonspecific stimulation in vitro. | it has been shown previously that lymphoid cells from mice which have rejected a tumor induced by the moloney sarcoma virus-leukemia virus (msv-mlv) complex develop high levels of specific h-2-restricted cytolytic t lymphocyte (ctl) activity after in vitro stimulation with syngeneic, irradiated mlv-induced lymphoma cells in mixed leukocyte-tumor cell cultures (mltc). attempts to further increase lytic activity by restimulating long-term mltc cells with syngeneic, irradiated lymphoma cells have m ... | 1979 | 221224 |
| genome organization of retroviruses. iii. restriction endonuclease cleavage maps of mouse sarcoma virus double-stranded dna synthesized in vitro. | genome length complementary dna (cdna) transcripts were synthesized in vitro by using purified virions of a cloned isolate of mouse sarcoma virus (msv clone 124). the cdna transcripts were converted to double-stranded form by utilizing dnase-digested calf thymus dna primers and e. coli dna polymerase i. restriction endonucleases sal i, hind iii, hpa i, bgl ii and xba i were found to cleave the msv double-stranded dna once to generate two fragments, whereas restriction endonucleases bgl i and hae ... | 1979 | 221890 |
| cycloheximide-dependent reversion of human cells transformed by msv and chemical carcinogen. | the protein synthesis inhibitor cycloheximide, at a concentration of 0.08 microgram per milliliter, induced flat morphology within 24 to 48 hours and low saturation density in human osteosarcoma cells transformed by kirsten murine sarcoma virus (ki-msv) or n-methyl-n' nitro-n-nitrosoguanidine. removal of the protein synthesis inhibitor caused both transformed cells to revert to the transformed phenotype. the demonstration of cell-surface antigens, cross-reacted with antiserums induced by extract ... | 1979 | 223242 |
| significance of the proline assay in the study of anti-msv cell-mediated immune reactions. | the cytolysis of 3h-proline-labelled tumour cells growing in monolayer by syngeneic immune lymphocytes has been studied in the murine sarcoma virus (msv) system. results show that the proline assay (pa) is a convenient way to reveal the activity of cytolytic t lymphocytes against fmr-like antigens. using the same effector and target cells, the classical chromium-release test (crt) fails to reveal any cytolytic activity, and the visual microcytotoxicity assay as well as several derived isotopic m ... | 1979 | 215185 |
| prevention of spontaneous leukemia in mice by oncornavirus immunization. | c57l, nih, and swr mice were immunized with inactivated gross leukemia virus (glv) and then mated with akr males. their f1 offspring were then immunized with the murine sarcoma virus pseudotype of glv, msv(glv). the concentrations of infectious ecotropic akr virus in tail extracts of immunized mice were 100- to 1,000-fold lower than in non-immunized controls when tested at 30--40 days of age. although viral titers increased slightly with time, the titers remained at least one log10 lower in the ... | 1979 | 216644 |
| a defined subgenomic fragment of in vitro synthesized moloney sarcoma virus dna can induce cell transformation upon transfection. | the longest dna molecules synthesized by endogenous reverse transcription in detergent-permeabilized moloney murine sarcoma virus (mo-msv) virions (clone g8-124) are double-stranded dna molecules of 5,8 kilobase pairs (kbp). this dna species has been purified by sedimentation of total in vitro synthesized mo-msv dna through neutral sucrose gradients. a physical map of the positions of the cleavage sites for a series of restriction endonucleases has been derived for this 5.8 kbp dna. mo-msv dna s ... | 1979 | 84715 |
| antiserum to murine leukemia virus recognizes novel cell surface molecules associated with growth control and transformation. | antiserum directed against murine leukemia virus also reacts with several external proteins present in rat cells transformed by a temperature-sensitive rous sarcoma virus. reaction of iodinated cell extracts with anti-mlv (murine leukemia virus) serum revealed the presence of a 200,000 dalton iodinated component detectable also by metabolic labelling with glucosamine only in serum-starved cultures restricted in the expression of transformation. a similar assay with iodinated cells that express t ... | 1979 | 86526 |
| cell-mediated reactivity to antigens shared by moloney-virus-induced lymphomas (lstra) and certain 3-methylcholanthrene-induced mouse sarcomas. | spleen cells (sc) both from balb/c mice whose primary moloney sarcoma virus (msv)-induced sarcomas had spontaneously regressed and from normal, untreated balb/c mice, were co-cultivated for 5 days with mitomycin-c-treated lstra cells; lstra is a balb/c moloney lymphoma which shares cell surface antigens with msv-indiced sarcomas. these sc, referred to as cmr and cu cells, respectively, were shown to be cytotoxic to lstra cells in 3 h 51cr-release assays; cmr cells showed, in most cases, the grea ... | 1979 | 86527 |