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fetal pathology in human parvovirus b19 infection.in a current netherlands study on the effects on mother and child of infection with the human parvovirus b19 during pregnancy, 10 pregnancies have been reported. three of them ended before term: two in fetal death and one by elective abortion. in two of these fetuses b19 infection in cells other than those of the erythroid series was demonstrated, and in the one terminated, ocular malformation and extensive inflammatory reactions in all fetal and placental tissues were found. the presence of b19 ...19892548567
aplastic crisis due to human b19 parvovirus infection in red cell pyrimidine-5'-nucleotidase deficiency.two siblings with chronic hemolytic anemia due to red cell pyrimidine-5'-nucleotidase (p-5'-n) deficiency, presented within a few days of each other with a febrile illness and pancytopenia. the cause of the aplastic crisis was an acute infection with human b19 parvovirus (b19 hpv) as proven by immunoelectron microscopy and dna hybridization. this is the first report on the association of b19-hpv-related aplastic crisis with p-5'-n deficiency.19892549757
seroprevalence of human parvovirus b19 infection in sao tomé and principe, malawi and mascarene islands.the prevalence of antibodies to human parvovirus b19 (anti-b19 igg) in sera (n = 577) from sao tomé and principe, malawi and mascarene islands (mauritius and rodriguez islands) was determined by antibody capture elisa. the b19 prevalence was 51.5% on sao tomé and principe, 58.4% in malawi, and 55.0% on the mauritius mainland and 2.2% on rodriguez island, indicating that b19 virus is highly prevalent not only in europe, japan and the americas but also in the african region. rodriguez islands has ...19892550018
hepatic disease associated with intrauterine parvovirus b19 infection in a newborn premature infant.a hydropic premature infant with intrauterine growth retardation died at 4 days of age and was found at necropsy to have advanced liver disease. clinical and serologic findings in mother and infant were consistent with recent parvovirus b19 infection. parvovirus can cause fetal liver disease in animals, and some instances of congenital hepatic dysfunction in man may be due to intrauterine parvovirus b19 infection.19892550601
anti-dna and antilymphocyte antibodies during acute infection with human parvovirus b19.sera from patients in the acute and recovery stages of parvovirus b19 infection, and from individuals with no detectable antiparvovirus antibody were examined for the presence of anti-dna and antilymphocyte antibodies. sixty-eight percent of individuals recently recovered from parvovirus infection had elevated levels of antidouble stranded (ds) and antisingle stranded (ss) dna antibodies. in addition, a cytotoxic igm antilymphocyte antibody was detected in more than 88% of these same sera. seria ...19892550630
erythema infectiosum in a primary school: investigation of an outbreak in bury.between january and april 1987, 97 of 302 (32%) children attending a primary school in bury, lancashire, were affected by an exanthematous illness. the duration and extent of the episode caused considerable local interest and anxiety. examination of 6 serum specimens obtained during the subsequent investigation demonstrated evidence of recent human parvovirus b19 infection in 3 children and 1 teacher. no evidence of recent rubella virus infection was found. the investigation of the episode is di ...19892552489
transient erythroblastopenia of childhood due to human parvovirus b19 infection. 19892553096
concurrent outbreaks of influenza and parvovirus b19 in a boys' boarding school.in the spring term of 1985 there was a protracted outbreak of upper respiratory tract febrile illness consistent with a clinical diagnosis of influenza in a boys' boarding school, which lasted from 23 january to 29 march. although influenza virus infection was confirmed in 89% of cases in the first half of the term, 53% of the cases which occurred in the second half of the term had no evidence of infection with influenza virus. between 5 february and 31 march 28 boys presented with skin rashes c ...19892553464
construction of a recombinant human parvovirus b19: adeno-associated virus 2 (aav) dna inverted terminal repeats are functional in an aav-b19 hybrid virus.to facilitate genetic analysis of the human pathogenic parvovirus b19, we constructed a hybrid b19 viral genome in which the defective b19 inverted terminal repeats were replaced with the full-length inverted terminal repeats from a nonpathogenic human parvovirus, the adeno-associated virus 2 (aav). the hybrid aav-b19 genome was rescued from a recombinant plasmid and then the dna was replicated upon transfection into adenovirus 2-infected human kb cells in the presence of aav genes coding for pr ...19892554300
[erythroblastopenia in children].the occurrence of isolated erythroblastopenia is a common problem in paediatrics, and in most cases three diagnoses may be considered: blackfan-diamond anaemia (congenital erythroblastopenia), transient erythroblastopenia of childhood and erythroblastopenia consecutive to parvovirus b19 infection. these three diseases have distinctive features: age of onset, association with other laboratory and clinical abnormalities and above all, transient or chronic character are of assistance in making an e ...19892554485
[erythroblastopenia caused by parvovirus in siblings, revealing a hereditary spherocytosis. apropos of a case].following the occurrence in two siblings of parvovirus b19-related acute transient erythroblastopenia with specific igms, hereditary spherocytosis (hs) was diagnosed for the first time in the two patients as well as in three other members of the family.19892554771
[erythema nodosum associated with parvovirus b19 infection]. 19892555812
polymerase chain reaction assay of parvovirus b19 dna in clinical specimens.the polymerase chain reaction (pcr) was used to detect parvovirus b19 dna in a panel of sera from individuals recently infected with b19, as demonstrated by the presence of anti-b19 immunoglobulin m. of 95 serum samples, 60 (63%) were found positive by pcr, whereas only 1 was also found positive by dot hybridization. in a control panel of 100 serum samples from individuals with other infections, only 1 serum sample was found positive by pcr, and this was also found positive by dot hybridization. ...19892556428
enzyme-linked immunosorbent assay for igg and igm antibodies against human parvovirus b19: use of monoclonal antibodies and viral antigen propagated in vitro.enzyme-linked immunosorbent assay (elisa) systems for serum igg and igm antibodies to human parvovirus b19 were established by utilizing anti-b19 monoclonal antibodies (mabs) and human plasma b19 antigen. the specificities of igg and igm elisa were confirmed by indirect immunofluorescence staining and western blot immunoassay with panel sera. the series of serum specimens obtained from two b19-infected patients were examined with elisa. the igm antibody titers increased quickly after the onset o ...19892559100
[aplastic crisis due to human parvovirus b19 infection in glucose-6-phosphate dehydrogenase deficiency].human parvovirus b19 is known to cause aplastic crisis in patients with hemolytic anemias due to cytotoxic effect of the infection to erythroid progenitor cells. we report here the first case of aplastic crisis by b19 in a patient with glucose-6-phosphate dehydrogenase deficiency. a five-year-old boy was admitted to the hospital because of severe anemia, fever and jaundice. four weeks after admission, he developed erythema infectiosum. b19 infection was confirmed using countercurrent immunoelect ...19892559569
parvovirus b19 infection in patients receiving cancer chemotherapy: the expanding spectrum of disease.initial reports have suggested that human parvovirus b19 infection in immune-compromised individuals may cause prolonged or chronic bone marrow suppression. we report four children receiving cancer chemotherapy who developed atypical patterns of cutaneous and hematologic disease associated with parvoviral infection. b19 infection was demonstrated by specific serologies. this report suggests that patients undergoing myelosuppressive or immunosuppressive therapy may have unusual manifestations of ...19892559623
persistent parvovirus b19 infections in humans. 19892560110
symptomatic parvovirus b19 infection and heat-treated factor ix concentrate. 19892566038
expression of the human parvovirus b19 protein fused to protein a in escherichia coli: recognition by igm and igg antibodies in human sera.a 1.4 kb fragment (nucleotides 2430 to 3901) encoding portions of the human parvovirus b19 structural proteins was inserted into the prit2 plasmid expression vector containing the gene encoding staphylococcal protein a under the control of the phage lambda promoter pr. the fusion protein was used to raise antibodies in rabbits. the sera were shown by immune electron microscopy to agglutinate b19 particles and were also shown to recognize the vp2 b19 capsid protein, by western blot analysis. the ...19892584954
a new peptide for human parvovirus b19 antibody detection.a serological assay for human parvovirus b19 was developed. linear overlapping synthetic peptides were synthesised according to parts of open reading frames 1 and 2. a region at the n-terminus of viral protein vp2 detected serological reactivity in indirect enzyme-linked immunosorbent assays for igg and igm with known seropositive human sera. a cyclized peptide taken from this region, amino acids 284-307, gave the best selective reactivity with seropositive and seronegative sera. the peptide ass ...19892617203
detection of the human parvovirus b19 in a blood donor plasma in rio de janeiro. 19892635755
propagation of human parvovirus b19 in primary culture of erythroid lineage cells derived from fetal liver.erythroid lineage cells derived from fetal liver were demonstrated to be target cells for human parvovirus b19 infection. b19 virus antigen-positive serum was inoculated into primary cultures containing erythroid lineage cells enriched from fetal liver. the b19 virus antigen was detected on about 5% of cells in the culture by immunofluorescence staining, and the stained cells were identified as erythroid lineage cells by double staining with anti-b19 virus-positive serum and anti-erythroid linea ...19892657097
characterization of monoclonal antibodies against human parvovirus b19.eleven hybridoma cell lines producing mouse monoclonal antibodies (mabs) against human parvovirus b19 were established. their specificity was as follows. approximately 5% of fetal erythroid cells inoculated with b19 reacted with all the mabs and with anti-b19 positive human serum, but not with negative serum by indirect double immunofluorescence staining. all the mabs recognized both vp-1 (84 kda) and vp-2 (58 kda) capsid proteins of b19 virions propagated in vitro and in vivo by western blottin ...19892671609
detection of parvovirus b19 dna in fetal tissues by in situ hybridisation and polymerase chain reaction.attempts were made to detect human parvovirus b19-dna by in situ hybridisation and the polymerase chain reaction in placental and fetal tissues from a case of intrauterine fetal death. in the in situ hybridisation experiments radioactive and non-radioactive (labelled with 2-acetyl-aminofluorene, aaf) dna probes were used. b19-dna was detectable in paraffin wax embedded fetal tissue from the liver, heart, lung, brain and thymus. the resolution with the aaf-labelled probes was higher than with the ...19892732347
transient expression of b19 parvovirus gene products in cos-7 cells transfected with b19-sv40 hybrid vectors.hybrid b19 parvovirus-sv40 origin vectors were transfected into cos-7 cells and replication of these plasmids studied. plasmids that have a frameshift mutation within the nonstructural gene region replicated to high level (copy number approximately 10,000/transfected cell) although somewhat lower than psvod, the sv40 origin vector without b19 sequence (copy number approximately 100,000/transfected cell). however, hybrid b19 parvovirus-sv40 origin vectors that do not contain these frameshift muta ...19892800343
parvovirus b19-induced perturbation of human megakaryocytopoiesis in vitro.parvovirus b19 infection leads to transient aplastic crises in individuals with chronic hemolytic anemias or immunodeficiency states. an additional unexplained sequela of b19 infection is thrombocytopenia. because b19 is known to have a remarkable tropism for human erythropoietic elements, and is not known to replicate in nonerythroid cells, the etiology of this thrombocytopenia is uncertain. we sought to define the pathobiology of b19-associated thrombocytopenia by examining the role of b19 on ...19902146978
detection of human parvovirus b19 dna by using the polymerase chain reaction.the polymerase chain reaction (pcr) was investigated for detecting human parvovirus b19 (b19) dna in sera. three pairs of oligonucleotides were evaluated as primers. the best oligonucleotide pair spanned 699 nucleotides, including the region common to vp1 and vp2. after pcr amplification of b19 dna in serum, a 699-nucleotide dna fragment was detected on agarose gels. this dna fragment was b19 dna, because after southern transfer it hybridized to a 19-nucleotide internal probe and contained a sin ...19902153700
numbness and tingling of fingers associated with parvovirus b19 infection. 19902153742
erythroid hypoplasia due to chronic infection with parvovirus b19. 19902154693
[human parvovirus b19 and pregnancy]. 19902154707
[fetal hydrops and parvovirus b19 infection]. 19902154708
[human parvovirus b19 and pregnancy]. 19902156179
use of mixture models in determining laboratory criterion for identification of seropositive individuals: application to parvovirus b19 serology.selecting the cut-off value to identify infected individuals requires a trade-off between sensitivity and specificity. the standard technique for selecting a cut-off uses results of a population either known or assumed uninfected. using mixture models, however, it is not necessary to identify an uninfected population. this technique also provides methods to estimate the probability that an individual will be accurately classified. we illustrate this technique in determining cut-off values for th ...19902156877
human parvoviruses.human parvovirus b19 is a common infection that causes a number of clinical illnesses, most of which are benign. however, in patients with a need for increased red cell production, a deficient immune system, or both (such as occurs in the fetus), it can be a life-threatening infection. intravenous immunoglobulin should be considered in the treatment of chronic b19 infection in immunodeficient patients and possibly in the management of other serious b19 infections. recent advances in the laborato ...19902156940
occupational risk of human parvovirus b19 infection for school and day-care personnel during an outbreak of erythema infectiosum.human parvovirus b19, the cause of erythema infectiosum, has recently been associated with adverse fetal outcomes. during a large outbreak of erythema infectiosum in connecticut, a survey was conducted on 571 (90%) of 634 school and day-care personnel to determine the risk of acquiring b19 infection. serologic evidence of b19 infection was determined by using an enzyme-linked immunosorbent assay. of the school and day-care personnel, 58% had evidence of previous b19 infection. the minimal rate o ...19902157074
the genome type of human parvovirus b19 strains isolated in japan during 1981 differs from types detected in 1986 to 1987: a correlation between genome type and prevalence.the genome dnas of 12 strains of human parvovirus b19, isolated in japan at two different times, 1981 and 1986 to 1987, were molecularly cloned in the plasmid puc18. the cloned b19 dnas were analysed by cleaving with restriction endonucleases, and were classified into several groups by genome type. the restriction endonuclease cutting patterns of b19 strains isolated during 1981 were similar to that of the group iv genome type, and the patterns of those isolated later were similar to that of gro ...19902157807
[parvovirus b19 infections in germany 1987-1988].3289 sera of patients were tested for parvovirus b19 specific antibodies (igm/igg) by elisa for routine virological diagnostics in 1987/1988. acute b19 infection could be confirmed in 14.5%, while 24.4% were already immune and in 61.1% antibodies could not be detected. only 0.12% of all sera were b19-dna positive by nucleic acid hybridisation. the highest rate of acute b19 infections could be detected in the age-group of the 6-15 year old. infections occurred above all in the period of january t ...19902158028
parvovirus b19-associated hemophagocytic syndrome.parvovirus b19 is a recently described pathogen, associated with an increasing spectrum of clinical manifestations. we present the first reported case, to our knowledge, of parvovirus b19-associated hemophagocytic syndrome, in which the diagnosis of parvovirus infection was documented by the presence of b19-specific igm and igg antibodies. pancytopenia resolved immediately following splenectomy and the patient recovered completely.19902158284
autoantibodies in sera from patients with parvovirus b19 infection. 19902159072
transmission of human parvovirus b19 by coagulation factor concentrates.the prevalence of antibody to human parvovirus b19 was determined in 86 children with congenital bleeding disorders. forty-seven of 53 boys (89%) receiving non-heat-treated factor viii or prothrombin complex concentrates were anti-b19 igg positive compared with 38% of their age-matched controls and 48% of children treated with cryoprecipitate. acute b19 virus infection occurred in 2 boys 3-4 weeks after they had received the same batch of commercial factor viii concentrate. of 11 susceptible chi ...19902160147
parvovirus infection and anaemia in a patient with aids: case report.opportunistic infections such as pneumocystis carinii pneumonia are well-recognised in patients with the acquired immune deficiency syndrome (aids). anaemia due to a variety of causes also occurs in aids. persistent infection with parvovirus (b19) causing severe anaemia has been reported in patients with leukaemia and congenital immunodeficiency. a case is now reported of parvovirus infection and anaemia, in an adult with aids, which responded dramatically to immunoglobulin therapy.19902160424
[parvovirus b19 infections. the cause of fifth disease-erythema infectiosum--can also cause aplastic crises, fetal damage and polyarthritis].human parvovirus b19 can be an important etiological factor in aplastic crises in patients with chronic hemolytic anemia, in fetal damage, and in acute polyarthritis. b19-virus can only be grown in cell cultures established from human bone marrow, where the virus production occurs in erythroblasts. parvovirus can cause severe, often fatal, infections in various kinds of animals, also in their fetuses, and many cases of teratogenic changes have been described. the b19-infection is diagnosed by de ...19902160748
[the fifth disease--erythema infectiosum. parvovirus infection in a family group].the causative agent of erythema infectiosum (ei), also known as the fifth disease, has been shown to be human parvovirus b19. this virus may also cause foetal damage, resulting in spontaneous abortion, hydrops foetalis or stillbirth. three cases of human parvovirus infection in a family are presented. the child has a classical rash. the father had a rash and joint-symptoms, which is common in adults. the mother had a subclinical infection, which is seen in the majority of ei cases. as the woman ...19902160749
prospective study of human parvovirus (b19) infection in pregnancy. public health laboratory service working party on fifth disease.to determine the fetal infection rate and outcome of pregnancy among women who acquire infection with human parvovirus (b19) in the antenatal period.19902161263
b19 chronic bone marrow failure: a persistent parvovirus infection of humans.the human parvovirus b19 may produce persistent infections with chronic bone marrow failure in immunocompromised hosts. despite poor data on the pathophysiologic basis of such infections, it is likely that immune humoral response plays an important part. indeed, administration of immunoglobulin preparation may help to control the course of b19 chronic infections.19902161513
fifth disease: role for nurses in pediatric practice.parvovirus b19 infection is a common childhood disease. infection is usually mild but may have serious consequences in pregnant women. nurses in pediatric practice may be the only health professionals in a position to recognize and intervene in this potentially serious condition in certain high risk individuals.19902163055
[seroprevalence of human parvovirus b 19 antibodies (sticker's disease/erythema infectiosum) in the drg population].we tested 638 human sera of all age groups (patients and blood donors) from the northern area of the g.d.r. and found 216 cases (33.9%) of specific parvovirus b19 antibodies (igg) by antibody capture elisa. the seroprevalence rose to 36.7% when the 18 parvo b19-igm- and/or parvo b19-dna-positive results were included. the antibody prevalence was 24.9 (27.8% resp.) in the 0-10 years group and increased continuously in the following age groups up to 61.1% (69.4% resp.) in the 51-60 years group. 50 ...19902164118
electron microscopic detection of human parvovirus (b19) in a patient with hiv infection.we report the findings on an hiv-positive patient found to be infected with human parvovirus b19. in a comparative study of sequential sera, direct electron microscopy (em) had the same sensitivity as dna hydridization for the detection of the virus. em did not require specific reagents and also yielded information on the state of the virus; i.e., whether random or complexed, and the type of antibody involved. the presence of parvovirus complicated the judgement as to drug treatment. this case a ...19902164559
development of a gene probe assay suitable for use in routine diagnosis: a pilot study using a dna probe for parvovirus b19.although dna probes have been widely applied in research for many years, lengthy and complex protocols, together with the use of radio-labelled probes, have hindered their introduction into routine diagnosis. in this study conventional methods were adapted to make gene probes more suitable for routine work and a biotin-labelled probe for human parvovirus b19 was utilised. the gene probe assay was shown to detect 0.25 pg of viral dna per 5 microliters of serum in 20 h. a comparative study was car ...19902166195
detection of parvovirus b19 by dot-blot and polymerase chain reaction.our studies compare detection of parvovirus b19 dna by dot-blot hybridization and by the polymerase chain reaction (pcr). compared to detection by dot-blot hybridization with a 32p-oligonucleotide probe, the sensitivity of pcr product detection by ethidium bromide fluorescence is not compromised when the size of the amplification target and number of cycles of amplification are properly selected. our pcr assay simultaneously amplifies two targets in the b19 genome and yields a sensitivity 10 tim ...19902166238
similarities in nucleotide sequence between serum and faecal human parvovirus dna.two dna clones were obtained from faecal specimens containing a parvovirus-like small round virus from a 1977 outbreak of gastroenteritis, and their nucleotide sequences were determined and found to be essentially identical with parts of the published sequence of serum parvovirus b19 and with a b19 isolate (jb) partially sequenced in this study. the clones corresponded mainly to genome regions coding for non-structural proteins, but also include a sequence of some 160 bp coding for structural pr ...19902166679
rheumatologic manifestations of human parvovirus b19 infection in adults. initial two-year clinical experience.during 1987 and 1988, we identified 9 adults at the medical and rheumatology services of the university of iowa hospitals and clinics who had a clinical diagnosis of fifth disease; 8 of the 9 had symptoms of joint involvement. another 12 adults with serologic positivity for anti-parvovirus b19 igm antibody presented with polyarthralgia/polyarthritis. patients were usually found to be seronegative for rheumatoid factor, and none developed nodules or erosive disease. many patients with chronic dis ...19902169746
an immunofluorescence assay for the detection of parvovirus b19 igg and igm antibodies based on recombinant viral antigen.an indirect immunofluorescence assay for serum igg and igm antibodies to human parvovirus b19 was established using recombinant b19 viral antigen, the capsid protein vp1, which had been produced in a baculovirus expression system. this protein gives a strong and characteristic signal in the immunofluorescence assay, making it a suitable candidate for this test system. the test results showed a good correlation with results obtained with a solid-phase capture radioimmunoassay (cohen et al., 1983) ...19902170436
the human parvovirus b19. 19902171481
diagnostic of parvovirus b19 infection.an elisa for detecting parvovirus b19 specific igm and igg was established using b19 viral particles as antigen derived from plasma of donors in the incubation phase of erythema infectiosum. at onset of erythema infectiosum anti-b19 igm was detected in titers of 10(-4) and was positive up to 19 weeks. anti-b19 igg could be detected in clinical specimens one week after onset of erythema infectiosum and persisted in titers up to 10(-6) throughout the convalescent period. among 3,289 sera of patien ...19902171483
detection of human parvovirus b19 dna by dot-hybridization and the polymerase chain reaction: applications for diagnosis of infections.the development of the polymerase chain reaction for the detection of human parvovirus b19 dna in clinical specimens is described. the sensitivity of this detection is compared with detection of virus using radiolabeled dna and rna probes. the polymerase chain reaction was the most sensitive, detecting approximately 10 fg b19 dna. several applications of the polymerase chain reaction for b19 dna detection are discussed: i) detection of b19 dna sequences in paraffin-embedded fetal tissues in a ca ...19902171484
polymerase chain reaction for detection of parvovirus b19 in immunodeficient patients with anemia.development of a polymerase chain reaction for detection of parvovirus b19 is described. among 14 primer pairs spanning the whole genome, 6 were shown to amplify viral dna at least 10(5) fold and 4 did not amplify non b19-dna to levels detectable by hybridization with a virus specific dna probe. failure to amplify only one b19 isolate with one particular primer combination suggests limited heterogeneity of the sequence among different viruses. examples for the application of the method are prese ...19902171485
the pathogenesis of diseases associated with b19 virus.b19 virus infection is common in the population and is frequently asymptomatic. however, a viraemia and prompt antibody response occurs in normal individuals and this is associated with mild, non-specific respiratory tract symptoms at the time of the viraemia and/or a rash-illness a week or ten days later. infection of red cell precursors is a regular occurrence and this leads to aplastic crisis if b19 virus infection occurs in an individual with chronic heamolytic anaemia. fetal infection somet ...19902171486
molecular biology and pathogenicity of human and animal parvoviruses.the so-called autonomous parvoviruses comprise a wide spectrum of viral agents with quite similar physicochemical and biological properties. characteristically, they are small, naked, and extraordinarily stable particles made up of only 2-4 structural proteins and a genome of linear, single-stranded dna consisting of 4500-5600 nts. for replication they depend on cellular helper functions which become available at distinct stages during cellular differentiation (developmentally-dependent function ...19902171487
risk of infection following exposures to human parvovirus b19.in response to concern about the effect of human parvovirus b19 infection of the fetus, we have developed estimates of the risk of adults becoming infected following b19 exposures at home, in schools or day-care centers, and in hospitals. these estimates can then be used with other data to estimate the risk to the fetus of a b19 exposure during pregnancy. the risk to the fetus equals the rate of maternal susceptibility to infection times the rate of maternal infection following the specific type ...19902171488
human parvovirus b19 and arthritis.parvovirus b19 has different clinical manifestations dependent on age and sex. in children it is usually a mild febrile exanthematous illness but in adults 60% of women and 30% of men have an arthropathy which may be severe and persistent. the pathogenesis of this arthropathy is not known. our studies establishing the clinical manifestations of parvovirus infection and examining their pathogenesis are reviewed.19902171489
parvovirus b19 infection in pregnancy.parvovirus b19 infection in pregnancy can cause hydrops fetalis resulting in fetal loss. acute b19 infection was serologically confirmed in 80 pregnant women by elisa. of 80 pregnancies, 4 were terminated. of the remaining 76 pregnancies, no fetal complications were observed in 36 (47.4%), hydrops fetalis occurred in 18 (23.7%) and no further information was available in 22 (28.9%). 15 of 18 (83.3%) fetuses with hydrops died. intrauterine transfusion was performed in the remaining three fetuses ...19902171490
parvovirus b19 infections in pregnancy.since 1986, 2,279 pregnant women were screened for anti parvovirus b19 igg and igm antibodies. of them 41% were seronegative, 54% had specific igg but no igm antibodies, indicating an infection in earlier life. acute infection could be demonstrated by igm antibody detection in 114 pregnant women (32% in the first, 54% in the second and 14% in the third trimester). hydrops fetalis occurred in 10 of the 114 pregnancies (8.7%). in five of these cases intrauterine exchange transfusion was done and u ...19902171491
successful intrauterine treatment of fetal hydrops caused by parvovirus b19 infection.the infection with parvovirus b19 during pregnancy causes in 1/3 of the cases an aplastic crisis in the fetus and consecutively generalized fetal hydrops as a result of severe anemia. some cases of fetal hydrops and intrauterine death are reported. in our two cases, the fetal therapy by intrauterine intravasal transfusion was successful and the children developed normal. the techniques and indications for fetal blood sampling and the method of intrauterine intravasal transfusion are explained.19902171492
immunoglobulins in the prophylaxis of parvovirus b19 infection. 19902172397
parvovirus and epidemic arthritis.five cases of acute polyarthritis in individuals with serologic evidence of recent human parvovirus b19 infection were seen in one medical practice. several individuals had had direct exposure to children with rash, and fifth disease was endemic in the community. all cases were associated with the presence of both igg and igm antibody to human parvovirus.19902172688
[a family with hereditary spherocytosis discovered after an infection with human parvovirus b19].hereditary spherocytosis was diagnosed in five of six children in one family after a human parvovirus b19 (b19-virus) infection. the diagnosis was made on the basis of severe anaemia, demonstrable haemolysis, decreased osmotic fragility and an increased number of spherocytes. since in the serum of the patients an increased level of igg and igm antibodies against b19 virus was detectable two weeks after the crisis it was concluded that the b19-virus infection caused the severe anaemia.19902172845
human parvovirus b19. 19902172896
human parvovirus b19 infections in pregnancy. 19902172907
pure red cell aplasia due to parvovirus b19 infection in a man with hiv infection.infection by parvovirus b19 is common and may become chronic if the patient is immunocompromised, leading to persistent erythroid hypoplasia. parvovirus should be added to the list of pathogens that can complicate the course of hiv infection and should be considered in the evaluation of severe anemia or red cell aplasia in any immunocompromised patient.19902174578
human parvovirus b19 arthropathy associated with desquamation. 19902174648
the prevalence of antibody to human parvovirus b19 in rio de janeiro, brazil.during 1985 and 1986 serum samples were collected from the rio de janeiro population and examined for the presence of igg antibody to human parvovirus b19. no difference in prevalence was found between males and females. antibody prevalence rose from 35% in children less than five years old to almost 80% in children aged eleven to fifteen years. the antibody prevalence in individuals over 50 years old was over 90%.19902175446
red cell aplasia associated with human parvovirus b19 and hiv infection: failure to respond clinically to intravenous. 19902175617
expression of an antigenic polypeptide of the human parvovirus b19.the dna fragment of the human parvovirus b19, with 715 nucleotides between nucleotide positions 3141-3856 was expressed in escherichia coli as a beta-galactosidase fusion protein. the plasmid vector pss20d used for this purpose permits cleavage of the viral gene product from the beta-galactosidase moiety by collagenase. after purification by p-aminophenyl-beta-d-thiogalactoside-sepharose and superose, a soluble protein with a molecular mass of 28 kda was isolated. it represents a common part of ...19902175835
[prevalence of anti-human parvovirus antibody in pregnant women].human parvovirus b19 (b19) has been shown to be associated with erythema infectiosum. recently, it was reported that when a pregnant woman is infected with b19, the fetus in her uterus sometimes becomes hydropic and results in a stillbirth. but no epidemiologic study of pregnant women in japan has been performed yet. we have established an in vitro propagation system of b19 virions and an assay system for detecting anti-b19 antibody by indirect immunofluorescence (if) staining. we examined the p ...19902179428
antigenic parvovirus b19 coat proteins vp1 and vp2 produced in large quantities in a baculovirus expression system.two baculovirus expression vectors derived from autographica californica nuclear polyhedrosis virus (acnpv) were prepared containing the complete 2.5 kb coding region for parvovirus b19 coat protein vp1 (acb19vp1l) and the 1.8 kb coding region for vp2 (acb19vp2l), placed under the control of the polyhedrin promoter. the recombinant viruses were used to infect spodoptera frugiperda cells and the proteins expressed were analysed using appropriate antibodies. acb19vp1l-infected cells produced b19 v ...19902188463
parvovirus b19. a workshop organized by the german association against virus diseases. april 1989. 19902222383
[a family with hereditary spherocytosis, discovered following an infection with human parvovirus b19]. 19902255366
nonstructural protein of parvoviruses b19 and minute virus of mice controls transcription.the genome of the human parvovirus b19 contains a transcriptional promoter (bp06) at map position 6, upstream from the nonstructural protein genes. by cotransfecting hela cells with this promoter cloned before the chloramphenicol acetyltransferase (cat) gene together with a plasmid containing almost the whole b19 genome, we showed that bp06 is transactivated by a b19 gene product. the transactivating viral protein was identified as the nonstructural protein ns-1. ns-1 synthesized in a wheat germ ...19902293668
susceptibility of human erythropoietic cells to b19 parvovirus in vitro increases with differentiation.b19 human parvovirus is the etiologic agent of transient aplastic crisis. to better understand b19 virus-induced hematopoietic suppression, we studied the host cell range of the virus using in vitro bone marrow cultures. first, b19 virus replication was examined in the presence of various purified cytokines using dna dot blot analysis. replication was detected only in erythropoietin-containing cultures. the other cytokines (granulocyte/macrophage colony-stimulating factor [gm-csf], g-csf, m-csf, ...19902404522
cloning of the human parvovirus b19 genome and structural analysis of its palindromic termini.we describe the molecular cloning of the entire 5.6-kb single-stranded dna genome of the human parvovirus b19 in bacterial plasmids. stable amplification of the recombinant plasmid dna was achieved in escherichia coli jc8111 but not in hb101 cells. sequence analysis of the cloned dna shows that the terminal 383 nucleotides at each end of the genome are identical inverted repeats. the distal 365 nucleotides of the repeat represent an imperfect palindrome which presumably folds over to form a hair ...19902408228
manifestations and treatment of human parvovirus b19 infection in immunocompromised patients.we diagnosed infections from human parvovirus b19 in three patients by using dot-blot hybridization and a polymerase chain reaction to detect b19 dna and using an enzyme immunoassay to detect igg and igm to b19. for 5 months a 5-year-old boy with acute lymphoblastic leukemia in remission had anemia without reticulocytes or bone marrow erythrocyte precursors. his serum lacked igg and igm to b19 but contained b19 dna. he received gamma globulin intravenously (0.4 gm/kg/day for 5 days); his viremia ...19901689774
pure red cell aplasia: review of treatment and proposal for a treatment strategy.the management of pure red cell aplasia (prca) continues to challenge clinical investigators because the pathophysiology is heterogeneous and poorly understood. there are five treatment regimens that have established efficacy for patients with chronic prca. in patients with congenital hypoplastic anemia the best results have been reported using corticosteroids. cyclosporine a is recommended as the treatment of choice in acquired prca. high-dose intravenous immunoglobulin therapy is highly effect ...19901698487
the production of human parvovirus capsid proteins in escherichia coli and their potential as diagnostic antigens.we have expressed a number of polypeptides derived from the capsid proteins of the human parvovirus b19 in escherichia coli. these include native vp1 (84k) and vp2 (58k) proteins and also fusions to beta-galactosidase containing differing amounts of the amino terminus of the vp1/2 polypeptide. although each of these was expressed at high levels and the majority were produced as full-length proteins, only one was soluble. this soluble polypeptide, p132, is a beta-galactosidase fusion protein that ...19901701479
[viral safety in hemotherapy. current aspects of hepatitis c and parvovirus b19 detection for plasma component therapy].a major risk of plasma component therapy was the transmission of infectious diseases. heat inactivation, tnbp/detergent- or beta-propiolactone/uv-treatment were introduced to reduce the risk of virus transmission, most notably those that cause hepatitis or aids. therefore we discuss topical problems of virus inactivation, particularly for the recently discovered hepatitis c-virus and the well-known parvovirus b19.19901703836
[molecular biological demonstration of parvovirus infection in fetal tissue].we report on the morphological findings in 16 fetuses with serologically confirmed maternal parvovirus b19 infection. typical routine morphological findings of the hydropic fetuses were the presence of abnormal erythroblasts with typical nuclear inclusions. these infected cells positively stained immunohistochemically with antibodies against a recombinant virus protein, as well as they ultrastructurally contained virus particles. using in-situ hybridization techniques, we were able to demonstrat ...19901708624
transfusion-transmitted diseases other than aids and hepatitis.although the major diseases transmitted by transfusion today are aids and hepatitis, many others also are known. these include cmv, syphilis, chagas disease, babesiosis, parvovirus b19, malaria, epstein-barr infection, and many others that have been reported only once or twice. reducing the risk of transfusion-transmitted diseases is a problem for donor centers where donor screening and laboratory testing for possible carriers is undertaken. physicians should be aware that the potential for dise ...19901963503
[infection with parvovirus b19: typical course of erythema infectiosum and its complications].severe chronic anemia, transitory aplastic crisis (tac), and lethal hydrops fetalis may be due to an infection with parvovirus b19 i.e. the causative agent of erythema infectiosum. 80% of the patients infected with b19 develop clinical symptoms and typically show the picture of erythema infectiosum--as one of our case reports (daughter) is going to illustrate. part of the cases, however, take a course without the characteristic slapped-face rash; here we frequently find acute arthritis and arthr ...19901964320
prevalence of human parvovirus (b19) and rubella virus infections in urban and remote rural areas in northern brazil.sera from inhabitants of belém, pará (542 sera), brazil and of members of 3 brazilian tribes--tiriyo/alto paru (near surinam) (212 sera), xicrin (128 sera), and mekranoiti (121 sera)--of different age and sex groups were tested for the presence of specific antibody against human parvovirus (b19) (ria) and rubella virus (latex agglutination test). parvovirus (b19) igg was found in 42.6% of the population sample from belém but in only 4.7 to 10.7% of the members of the tribes. rubella virus antibo ...19901964474
blood donor screening for parvovirus b19.a programme of blood donor screening for parvovirus b19 was conducted from january to may 1990. the main aim of the study was to identify a b19 positive donation that could be used as a source of viral antigen for diagnostic serology. out of 24,000 donors tested one was positive for b19 antigen by counter current immunoelectrophoresis and over 100 ml of undiluted b19 containing material was obtained. however, much of the positive donation was incorporated in a plasma pool of 28 donations. an aci ...19901964942
[human infection with parvovirus b19]. 19901966120
parvovirus b19 infection in the fetus. 19901974973
intrauterine blood transfusion for non-immune hydrops fetalis due to parvovirus b19 infection. 19901975307
in situ hybridization for the detection of human parvovirus b19 nucleic acid sequences in paraffin-embedded specimens.parvovirus infection of pregnant women leading to a transplacentar infection of the fetus may result in hydrops fetalis, and ultimately in intrauterine death of the fetus. in situ hybridization with a biotinylated as well as with a 35s-labeled probe for human parvovirus b19 was performed on formalin-fixed paraffin-embedded (ffpe) tissue from a fetus suffering from non-immunologic hydrops fetalis. histology was suggestive of viral infection probably with human parvovirus. parvovirus dna could be ...19901980555
[significance of viruses in maternal-child morbidity].in developing countries infectious diseases continue to be the primary cause of morbidity in children and they are also responsible for an increasing number of untimely deaths. viral, bacterial, mycotic, and parasitic agents are responsible for various pathological conditions that are present in the most vulnerable groups of the population. poverty, crowding, lack of access to potable water, low level of education, and inadequate disposition of excreta provide an ideal environment for infectio ...199012291425
in vitro propagation of parvovirus b19 in primary foetal liver culture.the culture of parvovirus b19 in foetal liver tissue has been described recently. we have established the technique in our laboratory and studied parameters affecting the yield of b19 virus. replication of the virus was detected by radioimmunoassay for b19 antigen and dot blot hybridization assay of b19 dna, and the virus was localized by immunofluorescence and thin section electron microscopy. b19 dna and antigen production became detectable at day 2 and reached a maximum at day 5. virus partic ...19912005439
analysis of splice junctions and in vitro and in vivo translation potential of the small, abundant b19 parvovirus rnas.two parvovirus b19 cdna libraries have been constructed; one from cos-7 cells transfected with a b19/psvod hybrid vector and the other from b19-infected human erythroid leukemic cells. we have used these libraries to investigate the expression of the abundant classes of polyadenylated b19 rnas; the 700- and 800-nt class which terminates in the middle of the genome and the 500- and 600-nt class which contains an orf from the extreme right-hand end of the genome. the 700- and 800-nt rna species we ...19912053277
recent advances in pediatric infectious diseases and their impact on dermatology.the past five years have seen numerous advances in the field of pediatric infectious diseases, and many of these have a substantial impact on the practice of dermatology. we review some of these advances and discuss their implications on etiology, diagnosis, therapy and complications of some relatively common conditions. the etiologic agent of exanthum subitum (roseola infantum) has been clearly implicated as a herpesvirus-6. although in the classically described situation high fever in a young ...19912061433
identification and mapping of neutralizing epitopes of human parvovirus b19 by using human antibodies.we identified and mapped the regions responsible for neutralization in the human parvovirus b19 structural protein by using region-specific human antibodies derived from seropositive blood donors. the region-specific antibodies were purified by using affinity columns coupled with synthetic peptides of the hydrophilic regions including the beta-turn structure deduced by the predicted secondary structure of vp2. fifteen highly specific antibodies against the synthetic peptides were obtained. ten o ...19911716693
detection of b19 parvovirus in human fetal tissues by electron microscopy.we present the electron microscopy observations on samples from 38 pregnancies that were investigated for b19 parvovirus infection. thirty-four had resulted in fetal loss thought to be due to a virus infection and 22 of the 38 were positive for b19 parvovirus in one or more of the tissues. twenty-one placentas and 75 fetal tissue samples were examined. fresh samples were investigated by immune electron microscopy while formalin-fixed tissues were examined as thin sections and by negative stainin ...19911722505
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