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adenovirus isolation and serology from wild bobwhite quail (colinus virginianus). 19816274295
egg transmission of avian adenovirus-associated virus and celo virus during experimental infections. 19816275751
avian adenoviruses and avian reoviruses isolated from diseased chickens. 19816275831
avian haemagglutinating adenovirus antibodies in australian poultry. 19816282245
growth of adeno-associated virus in embryonated eggs. 19816262350
[study of viral contamination of japanese quail and cell cultures from their embryos].agents identified as adenovirus celo were isolated from organ suspensions of 1500 japanese quails (jq) in 4 experiments. no contaminating viruses were found in examinations of cell cultures prepared from the kidneys of 80 jq. negative results were obtained in examinations of 163 antigens from jq organs in the cofal test. agents identified as mycoplasma were isolated in 16 cases from 736 specimens of the virus-containing fluid used for manufacture of measles virus. according to the results of the ...19816266156
hemorrhagic enteritis: virus distribution and sequential development of antibody in turkeys.turkeys poults were inoculated intraperitoneally with hemorrhagic enteritis virus (hev) at 4-1/2 weeks of age. antibody response and sequential development of viral antigen in various tissues were monitored. an enzyme-linked immunosorbent assay (elisa) was developed to study antibody production, and immunoperoxidase staining was used to determined sites of localization of the viral antigens in tissues. results of elisa and immunodiffusion tests were compared. elisa detected antibody from day 3 p ...19816266390
turkey respiratory tract adenoviruses: oncogenic potential in neonatal hamsters (mesocricetus auratus).the tumorigenic properties of 3 turkey adenoviruses (cua, nc-k, and mst) isolated from turkeys with respiratory tract disease and injected into neonatal hamsters (mesocricetus auratus) have been determined. one of 30 adenovirus isolates (cua) induced tumors at the site of inoculation (subcutaneously or intracranially) in neonatal hamsters. the tumors were identified as fibrosarcomas and undifferentiated sarcomas. the tumors were found to be free of infective virus, but hamsters which had tumors ...19816267964
turkey respiratory tract adenoviruses: purification and some biochemical properties. 19816267965
turkey respiratory tract adenoviruses: in vitro infectivity of purified dna. 19816267966
some biological and physico-chemical properties of egg drop syndrome (eds) avian adenovirus strain b8/78.the eds adenovirus produced either in the allantoic cavity of embryonated duck eggs (a0 virus) or in chicken embryo liver cell cultures (tc0 virus) were comparatively studied by gradient ultracentrifugation in cscl. the a0 viral particles banded at densities of 1.36 and 1.31 g/ml, whereas, the tc0 viral particle were found at densities of 1.33 and 1.31 g/ml, respectively. the heavy populations contained infectious and hemagglutinating particles if they were not pelleted and sonicated before grad ...19816268020
characterization of two strains of fowl adenovirus type 1.an adenovirus isolated from the trachea of a chicken with respiratory disease was compared with an isolate obtained from the trachea of a clinically normal chicken. the isolates caused similar respiratory disease experimentally but differed in plaque morphology and fluorescent-antibody reactions in infected chick embryos.19816279068
histopathology of avian adenovirus group ii splenomegaly of chickens.chickens were infected with an avian adenovirus group ii isolate previously obtained from chickens exhibiting splenomegaly in commercial broiler flocks. the isolate was inoculated orally in 6-week-old experimental chickens, which were euthanatized and necropsied 6 days postinoculation. the primary gross lesions found were splenomegaly and splenic mottling. numerous tissues from 12 chickens were taken for histologic evaluation. histologic lesions included splenic reticuloendothelial cell hyperpla ...19816279073
immunological relationship among adenoviruses of humans, simians, and nonprimates as determined by the neutralization test.98 antisera prepared against 25 simian adenoviruses and 31 adenovirus types from various nonprimate mammals and birds were tested for their ability to neutralize 35 human adenovirus prototypes. antisera to these human adenovirus types were similarly tested against 25 simian adenovirus prototypes. although very few reactions to the human adenoviruses were found in antisera to nonprimate viruses, cross-reactions among the simian and human adenoviruses were more frequent. the majority of these reac ...19816168602
[a contribution to the egg drop syndrome of laying chickens. 1: clinical and serological findings (author's transl)]. 19826280951
[a contribution to the egg drop syndrome of laying chickens. 2. macroscopic and histological findings (author's transl)]. 19826280952
isolation of a haemagglutinating adenovirus from commercial ducks. 19826281959
[oncogenicity of adenoviruses]. 19826298900
survey on antibody against hemorrhagic enteritis virus among chicken flocks in japan.a survey on antibody against hemorrhagic enteritis virus was conducted by the agar-gel precipitation test among chicken flocks in japan. the positive rate of individual serum samples increased with the advance in age of chickens. ninety-one of 181 sera (50%) and 23 of 33 flocks (79%) were positive among chickens more than 181 days old.19826312333
sequence homology between avian and human adenoviruses.studies of hybridization between fowl adenovirus type 1 (chicken embryo lethal orphan virus) dna and human adenovirus type 2 dna revealed two short but distinct regions which cross-hybridized under stringent conditions. one of the homologous regions was located between map positions 18.1 and 19.3 and did not correspond to any gene recognized so far. the second region mapped in the hexon gene between position 57 and 58.19826283148
is avian adeno-associated virus an endogenous virus of chicken cells?the adeno-associated viruses (aav) are defective parvoviruses which produce infective progeny only in cells co-infected with a 'helper' adenovirus (ad). both human and simian aav have been recovered from human and simian primary cell cultures following their inoculation with 'aav-free' ad. whereas some studies have suggested that aav exists in a latent state in these cells, others have indicated that the aav genome is capable of establishing and maintaining a latent state in defined laboratory c ...19826285201
the effect of incubation temperature on the propagation of duck adenovirus (virus 127-like) in duck and chicken cells.duck adenovirus (cornell strain) was propagated in duck and chicken embryo cells at 37.5 c and at 40 c. in duck cells, virus levels, as indicated by ha titers, peaked earlier at 40 c than at 37.5 c. high titers were eventually observed in duck cells at both temperatures. in chicken embryo fibroblasts, no titers were observed at 37.5 c, whereas low titers were observed at 40 c. evidence of virus propagation was not detected in chicken embryo liver and kidney cells.19826285881
the fibers of fowl adenoviruses.purified virions from 14 strains of fowl adenoviruses (fav) representing 11 serotypes were examined by electron microscopy. pentons of all strains turned out to have two projections at their penton bases. it is shown that the double fibers also protrude from the intact virion. the length of both fibers was measured on free pentons and is given for each serotype. double fibers seem to be a common feature of fav. the serologically unrelated egg drop syndrome (eds) avian adenovirus strain 127 was c ...19826287974
a common sequence in the inverted terminal repetitions of human and avian adenoviruses.the termini of the avian chick embryo lethal orphan (celo) virus dna have been sequenced. the results revealed a 63-bp-long inverted terminal repetition (itr) which shared the sequence ataata with all adenovirus termini, thus far analyzed. the celo virus itr differed from those of the mammalian adenoviruses in two major aspects: (i) it is not a perfect duplication; (ii) it begins with a 5'-guanylic acid residue instead of the cytidylic acid normally observed in adenoviruses.19826290323
survey on antibody against egg drop syndrome-1976 virus among chicken flocks in japan.a survey on antibody against egg drop syndrome-1976 virus was carried out with 4,518 sera collected from 667 flocks over a period from 1971 to 1981 in japan. antibody-positive sera were initially detected in 1976, and the number of positive sera increased suddenly in 1979, and a total of 270 sera from 23 flocks were positive. the positive flocks consisted of 20 broiler parent flocks belonging to 9 farms and 3 layer flocks belonging to 2 farms. it seemed from the results of this survey that the p ...19826290899
a survey of serum antibody to avian haemagglutinating adenovirus in queensland poultry flocks. 19826291500
serological examination and egg production of progeny of fowl experimentally infected with egg drop syndrome 1976 virus.following eds'76 virus (bc14 virus) infection of breeder chickens by the conjunctival route, vertical transmission occurred in the first week after infection. in the progeny which had been infected with eds'76 virus by the vertical route, increasing haemagglutination inhibiting (hi) titres to bc14 virus and increasing numbers of birds with hi titres were observed from 3 weeks to 15 weeks of age. sixty-one per cent of the hens and 77 per cent of the cocks had 2 log hi bc14 virus titres exceeding ...19826293156
lesions induced in the respiratory tract of chickens by serologically different adenoviruses.ten strains of adenovirus representing 10 serotypes were administered intratracheally to two groups of 3-week-old specific-pathogen-free chickens. birds in group 1 were given only adenovirus. birds in group 2 were inoculated with a virulent infectious bursal disease virus (ibdv) by eye-drop at one day of age as well as with the adenovirus at 3 weeks. the chicks were examined daily. respiratory rales were observed in some birds with the dual infection. gross pathologic alterations were minimal an ...19826293441
heat inactivation of a duck adenovirus serologically indistinguishable from adenovirus 127. 19826293448
pulmonary congestion and edema (marble spleen disease) of chickens produced by group ii avian adenovirus."marble spleen disease" of chickens was diagnosed in 22-week-old chickens. total mortality was 8.9%. deaths occurred over a period of 2 months. gross lesions included pulmonary congestion, splenomegaly, hepatomegaly, and congestion of egg follicles. microscopic lesions included pulmonary congestion and edema, and reticuloendothelial cell hyperplasia of the spleen with concurrent white-pulp necrosis and lymphocyte depletion. the pulmonary lesions were of sufficient intensity to have caused the de ...19826293450
serological cross-reactivity of avian adenovirus serotypes in an enzyme-linked immunosorbent assay.fowl adenoviruses free of avian adenovirus-associated virus, representing 10 serotypes, were tested for cross-reactivity in an enzyme-linked immunosorbent assay (elisa). all antigens and antisera prepared in chickens, along with uninfected control antigen and normal chicken serum, were reacted in a checkerboard pattern with elisa. there was considerable cross-reactivity among all serotypes tested. homologous reactions were generally, but not always, stronger than heterologous reactions. elisa wa ...19826297452
the oncogenicity of avian adenoviruses. iii. in situ dna hybridization of tumor line cells localized a large number of a virocellular sequence in few chromosomes. 19826278719
adenovirus-related rna sequences in human neurogenic tumours.thirty two human tumours, mainly neurogenic, have been investigated for the presence of adenovirus-related rna sequences. 3h-labelled tumour virus dna probes derived from human adenoviruses types 2 and 12, bovine adenovirus type 3, and avian adenovirus celo were hybridized in-situ on tumour kryostat sections under conditions that detect complementary rna. tumour virus-related rna was detected in 62% of all tumours tested, but was not detectable in normal human brain tissues. expression of tumour ...19826278813
biochemical studies of celo virus: an oncogenic avian adenovirus. 19836297961
isolation of fowl adenovirus from tendons and tendon sheaths of chickens with leg weakness. 19836314026
comparison of enzyme-linked immunosorbent assay with hemagglutination-inhibition and immunodiffusion tests for detection of antibodies to a hemagglutinating duck adenovirus in chickens.an indirect enzyme-linked immunosorbent assay (elisa) for the detection of antibody to a hemagglutinating duck adenovirus is described. optimum conditions for the test were determined, and the system was compared with procedures currently used. experimentally infected chickens were assayed for specific antibody by elisa, hemagglutination inhibition (hi), and immunodiffusion (id). the elisa was a sensitive and reliable method for detecting antibody, although positive titers were not always in agr ...19836314978
hemorrhagic enteritis in captive american kestrels (falco sparverius).hemorrhagic enteritis and hepatitis of suspected adenovirus etiology were the apparent cause of death of nine captive american kestrels. cloacal hemorrhage was the only prominent gross lesion: disseminated hepatocellular necrosis, and intranuclear inclusion bodies were evident microscopically. electron microscopy revealed numerous adenovirus-like particles associated with the hepatic lesions. attempts to serologically identify the agent were unsuccessful.19836315970
isolation and identification of avian adenovirus from a case of inclusion body hepatitis in broilers in malaysia. 19836317137
isolation of the virus of egg drop syndrome 1976 (eds-76) in a japanese outbreak. 19836317930
comparative sequence analysis of the inverted terminal repetition in the genomes of animal and avian adenoviruses.the nucleotide sequences at the inverted terminal repetitions from two animal adenoviruses, infectious canine hepatitis virus and equine adenovirus, and from one avian adenovirus, celo, were analyzed. dnas from infectious canine hepatitis virus and equine adenovirus contain a homologous region which is 23 nucleotides long from the terminus. the first 17 nucleotides of this region are identical to the ones in human adenovirus type 2 dna. the striking homologous sequence of 14 nucleotides, conserv ...19836301151
effects of experimental infection of fowl with eds'76 virus, infectious bronchitis virus and/or fowl adenovirus on laying performance. 19836302974
a comparison of the terminal protein and hexon polypeptides of avian and human adenoviruses.it was found that the virion terminal protein of chick embryo lethal orphan (celo) virus had a molecular weight of 46,000, and the hexon a molecular weight of 100,000. 125i-labelled tryptic and chymotryptic peptide maps of the hexons and terminal proteins from celo virus and human adenovirus type 5 (ad5) differed. however, limited proteolysis of celo virus and ad5 terminal proteins by protease v8 showed similarities which were not detected in the case of the two hexons.19836304239
the use of an enzyme-linked immunosorbent assay to detect igg antibodies to serotype-specific and group-specific antigens of fowl adenovirus serotypes 2, 3 and 4.a sensitive enzyme-linked immunosorbent assay (elisa) has been developed which can detect serotype and group-specific antibodies (igg) to fowl adenovirus serotypes 2, 3 and 4. the chickens produced principally type-specific antibodies after a single oral inoculation of virus which enabled that strain to be identified by the elisa. however, inoculation of an heterologous serotype, although inducing strain-specific antibodies to itself, also induced high levels of antibody to the group-specific an ...19836330150
isolation of chicken anemia agent with mdcc-msb1 cells from chickens in the field.an attempt was made to isolate chicken anemia agent (caa) from chickens suffering from anemia in the field by using mdcc - msb1 , which was an established cell line derived from marek's disease lymphoma. when 99 chickens of 15 flocks were examined, caa was isolated from 58 chickens of 12 flocks. the rate of caa isolation with mdcc - msb1 cells was almost the same as that determined by an in vivo method by chick inoculation. it was shown that caa was more closely concerned with anemic diseases of ...19836330575
purification and hemagglutinating properties of egg drop syndrome 1976 virus.we purified three populations of virus particles, f7, f9 and f17, with buoyant densities of 1.34, 1.33 and 1.29 g/ml, respectively, in cscl equilibrium density gradients from cultures of chick embryo liver cells infected with the h-162 strain of the virus of egg drop syndrome 1976. f9 particles were infectious complete virions and most f17 particles were empty particles. f7 particles were less infectious, and had little capacity of hemagglutination (ha). ha titers were the same at 4 degrees and ...19846322727
[amplification of virocellular sequences avian adenovirus-induced tumor cells].adenovirus-induced tumor cells (including transformed cells) examined contain a part of the viral genome. however, number of molecules of the virus dna persisting in a cell varies in the tumor cell lines. tumor cell lines which were induced by celo virus, an avian adenovirus, carried the virus dna in the range from 1 to 920 molecules per cell. of the tumor cell line which carried large numbers of molecules of the virus dnr, one tumor cell line, rt 13 (160 molecules/cell), was studied both at the ...19846324694
evidence of exposure of waterfowl and other aquatic birds to the hemagglutinating duck adenovirus identical to eds-76 virus.serum and fecal samples from 12 species of aquatic birds were studied for evidence of exposure to a hemagglutinating duck adenovirus (dav). dav is serologically indistinguishable from egg-drop syndrome-76 virus. a total of 285 serum samples were tested by the hemagglutination-inhibition (hi) test. forty-two percent of the birds had hi antibodies, with titers ranging from 8 to 256. wild ducks showed the highest frequency of antibodies (56%) while in coots and grebes, antibody was less frequent, 3 ...19846325725
pathogenicity of various adenovirus serotypes in the presence of escherichia coli in chickens.ten strains of adenovirus representing 10 serotypes were administered intratracheally to 3-week-old specific-pathogen-free chickens, which also received 2.9 x 10(5) colony-forming units of a pathogenic escherichia coli intranasally. one group was given only e. coli, and one was retained as an uninoculated control. gross pathologic alterations post-mortem were minimal and limited to multiple scattered, pale areas in the lungs of an occasional chicken in various groups. histopathologic changes in ...19846326735
experimental infection of laying chickens with adenovirus 127 and with a related virus isolated from ducks.infection of commercially reared white leghorn and white rock hens with adenovirus 127 was associated with decreases in total egg production, external egg quality, egg weight (p less than 0.01), and eggshell thickness (p less than 0.01). the egg-production and egg-quality disturbances were transient, and production returned to normal approximately 4 weeks postinfection. infection of white leghorns with a hemagglutinating adenovirus isolated from missouri ducks did not adversely affect egg produc ...19846326738
efficacy and safety of a cell-culture live virus vaccine for hemorrhagic enteritis of turkeys: laboratory studies.poults free from hemorrhagic enteritis (he) antibody were vaccinated by gavage at 1 day or 2 weeks of age with a live he vaccine virus that had been propagated in a marek's disease (md)-induced b-lymphoblastoid cell line of turkey origin. vaccinated and unvaccinated poults were challenged with a virulent he virus at various times postvaccination. one hundred tissue-culture-infectious doses of the vaccine virus per poult were sufficient to induce a serological response as well as to protect poult ...19846326740
grouping of fowl adenoviruses based upon the restriction patterns of dna generated by bamhi and hindiii.based upon the restriction patterns of dnas generated by restriction endonucleases bamhi and hindiii, 17 fowl adenovirus strains representing 11 serotypes were placed into 5 groups: group a, strains celo and 112; group b, strains 340 and tr-22; group c, strains kr-5 and c-2b; group d, strains 380, 685, sr-48, sr-49, and a-2; and group e, strains yr-36, cr-119, x-11, h-6, tr-59, and 764.19846090337
viral hepatitis in domestic geese in saskatchewan.up to 25% mortality occurred in goslings between 8 and 28 days of age in three sequential hatches from a closed breeder flock on a single farm. the birds died suddenly with minor ascites, subcutaneous and myocardial hemorrhages, and pale, mottled livers with petechial hemorrhages. liver necrosis associated with intranuclear inclusion bodies was seen on histology. virus particles were found on examination of the inclusion bodies with an electron microscope. liver homogenates caused variable morta ...19846091609
the structural proteins of chick embryo lethal orphan virus (fowl adenovirus type 1).chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contains at least 14 structural proteins with polypeptide molecular weights ranging from 100k to about 6k. a nomenclature of the celo virion polypeptides is presented and the molar proportion of each polypeptide has been estimated. the celo virus pentons were specifically released from the virion by dialysis against borate-based calcium-magnesium saline. the penton base (polypeptide iii, mol. wt. 92k) and two fibres were separated, ...19846092524
dna-binding proteins of chick embryo lethal orphan virus: lack of complementation between early proteins of avian and human adenoviruses.chick cells infected by chick embryo lethal orphan (celo) virus (fowl adenovirus type 1) contained four prominent virus-specific, structurally related dna-binding proteins with mol. wt. of 74k, 64k, 56k, 52k, and two minor forms. the celo virus dna-binding proteins were phosphorylated, delayed-early nuclear proteins. celo virus early proteins were expressed in bhk cells, but did not complement human adenovirus type 5 mutants with lesions e1a, e2a or e2b. moreover, celo virus dna-binding proteins ...19846092525
structural organization and polypeptide composition of the avian adenovirus core.celo virus (fowl adenovirus 1) contained three core polypeptides of molecular weights 20,000, 12,000, and 9,500. the core was similar to that of human adenoviruses, with some evidence of compact subcore domains. micrococcal nuclease digestion of celo virus cores produced a smear of dna fragments of gradually decreasing size, with no nucleosome subunit or repeat pattern. moreover, when digested cores were analyzed without protease treatment, there was again no evidence of a nucleosome substructur ...19846092686
adenovirus-like agent in the bursa of fabricius of herring gulls (larus argentatus pontoppidan) from newfoundland, canada. 19846092730
adenovirus-like particles associated with intranuclear inclusion bodies in the kidney of a common murre (uria aalge).adenovirus-like particles were identified by transmission electron microscopy in intranuclear inclusion bodies in the renal collecting tubules of a male common murre. the bird had been trapped in an oil-spill and had been cleaned and held in captivity before euthanasia. the presence of the virus appeared to be causing little or no renal disease. it is thought that this bird suffered activation of a latent viral infection as a result of the stress of oil intoxication, handling, and confinement.19852985038
purification method for the avian adeno-associated virus.avian adeno-associated virus (aaav) pre-purified by uvasol extraction, one discontinuous and two cscl equilibrium density gradients, was still considerably contaminated with avian adenovirus (celo strain). four different approaches were investigated in attempts to improve the elimination of the contaminating celo virus. the contaminations were assayed by double immunodiffusion and indirect immunofluorescence. the immunoprecipitation of celo virus with antiserum and protein a-sepharose is the mos ...19852989313
increase in dna polymerase alpha in nuclei of chicken kidney cells after avian adenovirus infection.dna polymerase activity which sedimented at about 7s in the presence of 0.5m kc1 increased in cultured kidney cells after infection with a chicken embryo lethal orphan virus, an avian adenovirus. elevated levels of antigenic material to a monoclonal antibody specific for the large subunit of chicken dna polymerase alpha were also detected in nuclei of virus-infected cells by an immunofluorescence method. since the elevations of dna polymerase activity and of nuclear immunofluorescence was not in ...19852991062
an investigation of vertical transmission of a fowl adenovirus serotype 8. 19852992432
use of egg yolk to determine antibody levels in chickens inoculated with a hemagglutinating duck adenovirus (adenovirus 127-like).chickens were experimentally infected with a duck adenovirus that has been shown to be serologically indistinguishable from adenovirus 127. sera and eggs were collected at intervals after exposure for antibody determination by the hemagglutination-inhibition (hi) test, the enzyme-linked immunosorbent assay (elisa), and the immunodiffusion (id) test. egg yolks were processed for use in the serological tests by (a) dilution in phosphate-buffered saline (pbs), (b) extraction of the water-soluble fr ...19852992438
haemagglutinating adenovirus in victorian chicken flocks 1980-83. 19852994619
experimental infection of laying hens with an adenovirus isolated from ducks showing eds symptoms. 19853012988
involvement of spleen components of mature fowl in the primary and secondary humoral antibody response following experimental eds'76 virus infection.cellular changes in spleens of mature fowl in relation to both the primary and secondary humoral antibody response following experimental eds'76 virus infection were studied. the influence of splenectomy on humoral antibody response was also examined. experimental fowl had been naturally infected with fowl adenovirus (fav) but did not possess precipitins to these viruses at the time of eds'76 virus infection. since eds'76 infection provokes a recall of the group antibody to fav, this infection s ...19863014709
pathology of avian adenovirus serotypes in the presence of escherichia coli in infectious-bursal-disease-virus-infected specific-pathogen-free chickens.ten strains of adenovirus representing 10 serotypes were administered intratracheally to 3-week-old specific-pathogen-free chickens, which also received 2.5 x 10(5) colony-forming units of a pathogenic escherichia coli intranasally. those birds had been inoculated by eye drop at 1 day of age with a virulent infectious bursal disease virus (ibdv). controls consisted of groups of chickens inoculated with: (a) ibdv and e. coli, (b) ibdv only, (c) e. coli only, and (d) no virus or e. coli. gross pul ...19863015109
a possible viral candidate for the aetiology of turkey rhinotracheitis. 19863006318
[detection of avian adenovirus antigens in chicken cell cultures]. 19863009130
va rnas from avian and human adenoviruses: dramatic differences in length, sequence, and gene location.human adenoviruses encode low-molecular-weight rnas, so-called va rnas, which are transcribed by rna polymerase iii. these rnas are required for an efficient translation of viral mrnas late after infection. the genes for the va rnas in the genome of celo virus were mapped and characterized. the results showed a number of surprising differences between celo virus and human adenovirus type 2 (ad2). thus, the celo virus genome encoded only one va rna species, in contrast to human ad2, which encoded ...19863009871
viruses associated with turkey rhinotracheitis in great britain. 19863012855
inclusion body hepatitis associated with adenovirus-like particles in a cockatiel (psittaciformes; nymphicus hollandicus). 19863026296
[sequelae of concomitant infection of chicks with adenovirus or reovirus and the agent of avian infectious anemia (caa)]. 19863031906
[stability of the structure and antigenic determinants of adenovirus type 1 native hexon to proteases].hexon capsomers of human adenovirus type 1 (h1) labeled by iodine 125 were digested in a native state (trimers) by trypsin, chymotrypsin or papain, and the resulting hydrolysates were analyzed by sds-page. in each case, a discrete and temporally stable pattern of relatively large fragments was revealed. the degree of hexon polypeptide hydrolysis was maximal for papain, intermediate for chymotrypsin and minimal for trypsin, the largest fragments in the digest being 32, 40 and 80 kd, respectively. ...19862429709
[adenoviruses in chickens: an attempt at summarizing]. 19873032149
[the pathogenesis of "egg drop syndrome 76" after experimental infection of one-day-old chicks and laying hens]. 19873032150
further studies on in vitro and in vivo assays of hemorrhagic enteritis virus (hev).isolation of hemorrhagic enteritis virus (hev) from spleens of infected turkeys in the mdtc-rp19 lymphoblastoid cell line was compared with detection of hev antigen in the same spleens using the agar gel precipitation (agp) test. a concordance of 80% was found between the two assays. virus isolation had a sensitivity of 84% and a specificity of 88% compared with the agp test. rp19 cells were also susceptible to infection with several other avian adenoviruses, but such infection was easily differ ...19873039962
enteric viral infections of turkey poults: incidence of infection.four flocks from one commercial market-turkey operation in ohio were monitored for the presence of enteric viruses. each flock was sampled at intervals from placement until at least 7 weeks of age; sampling was more frequent in the first 4 weeks of life. the earliest infections detected were astrovirus infections or combination infections of astrovirus and rotavirus-like virus (rvlv) or astrovirus and rotavirus. during the first 4 weeks of life, astrovirus was the most frequently detected virus, ...19873039964
a trivalent antigen for the detection of type i avian adenovirus precipitin.a double immunodiffusion antigen prepared from cell-culture-propagated celo virus was not capable of detecting precipitin directed against all of the type i avian adenovirus (fowl adenoviruses) isolates tested. however, an antigen pool containing celo-4, b-3, and ibh-2 (tipton) fowl adenovirus isolates detected precipitin directed against representative isolates of 10 type i serotypes. additionally, this antigen pool markedly improved detection of adenovirus field exposure.19873039966
study of avian adenovirus dna infectivity in chick embryos.inoculation of celo adenovirus deproteinized dna into the allantoic cavity of 9-day-old chick embryos (ce) induced the synthesis of infectious viral particles. the produced virions appeared to be identical with celo adenovirions in terms of morphology, electrophoretic and immunochemical properties of hexon major capsid protein and also of dna dot-hybridization. high infectivity of celo dna (minimal infective dose equaled 40 ng) may be also related, at least in part, to the absence of deoxyribonu ...19872886031
adenovirus infection associated with respiratory disease in commercial chickens.the lesions and etiologic agents associated with 13 outbreaks of respiratory disease in commercial chickens were investigated. adenoviruses were isolated from tracheal and lung tissues of affected chickens in all 13 outbreaks. escherichia coli was isolated from the lung of an occasional bird. the tracheal specimens were consistently negative for bordetella avium, but e. coli and occasionally staphylococcus aureus were isolated. there was also serological evidence in one outbreak, and pathologica ...19872823779
inclusion body hepatitis in bobwhite quail (colinus virginianus).farm-reared bobwhite quails less than 3 weeks of age experienced high mortality (250 of 400). at necropsy, these birds had multiple 1-to-2-mm pale foci throughout their livers. histologically, these foci varied from acute hepatocellular necrosis without an inflammatory response to necrosis with infiltrates of mononuclear inflammatory cells and some heterophils. hepatocytes adjacent to affected areas had large basophilic intranuclear inclusions. a group i avian adenovirus was isolated from affect ...19872823780
preferential clustering of viral dna sequences at or near the site of chromosomal rearrangement in fowl adenovirus type 1 dna-transformed cell lines.all six transformants obtained by inoculating fowl adenovirus type 1 (celo virus) dna or its fragments into a rat cell line of normal karyotype had more than 50 copy-equivalents of viral dna sequences. each of the transformants had almost all if not all of these viral dna sequences clustered on a marker chromosome(s). although the marker chromosome(s) differed from one cell line to another, viral dna sequences preferentially clustered in or near the achromatic (or light-stained) region of the g- ...19873023693
pathogenicity studies with a strain of fowl adenovirus serotype 8 (vri-33) in chickens.experiments were undertaken to study the pathogenesis of vri-33, a strain of fowl adenovirus serotype 8 isolated from the liver of a broiler chicken with inclusion body hepatitis. a 30% death rate resulted from oral infection of one-day-old specific pathogen free chickens with 10(6) plaque forming units of vri-33. chickens 10, 14, 21 and 28 days of age did not die following infection via natural routes but there were some motalities following infection via parenteral routes. immunodepression by ...19872838009
chicken embryo propagation of type i avian adenoviruses.forty-two clone-purified, cell-culture-propagated type i avian adenoviruses (aav) representing 11 serotypes and two intermediate strains were evaluated for virus replication (evidenced by embryo death and lesions) resulting from the inoculation of specific-pathogen-free chicken embryos via the chorioallantoic sac or yolk sac. commonly observed embryonic changes were death, stunting and curling, hepatitis, splenomegaly, congestion and hemorrhage of body parts, and urate formation in the kidneys. ...19882840884
[cloning of terminal fragments of the avian adenovirus celo genome and isolation of a recombinant plasmid carrying the viral oncogene].the terminal fragment of avian adenovirus celo has been cloned in a plasmid vector. the obtained recombinant plasmid pcbe1 carries the terminal bamhi-e fragment of celo dna. transfection of a nonpermissive culture of rat2 cell line by the plasmid dna results in formation of transformation focuses. the cloned bamhi-e fragment of celo dna is concluded to contain the viral oncogene. thus, the celo genome region deriving the bamhi-e fragment is "left".19882842669
some observations on the response of ring-necked pheasants to inoculation with various strains of cell-culture-propagated type ii avian adenovirus.the response of ring-necked pheasants to inoculation with three strains of cell-culture-propagated type ii avian adenovirus was examined. marble spleen disease (msd) virus of pheasants and both avirulent and virulent strains of hemorrhagic enteritis virus (hev) of turkeys all induced typical gross and microscopic splenic lesions of msd; neither msd-associated lung lesions nor mortality were noted in inoculated pheasants, regardless of strain of virus used. pheasants inoculated with a cell-cultur ...19882848487
inclusion-body hepatitis in day-old turkeys.inclusion-body hepatitis was diagnosed in 1-day-old turkeys experiencing above-average mortality. at necropsy, turkeys appeared anemic and had pale yellow livers. histopathologic examination of affected livers revealed diffuse hepatic degeneration and multifocal necrosis, with approximately 70% of the hepatocytes containing large, basophilic, intranuclear inclusion bodies. an adenovirus was isolated from affected livers and identified as a group i avian adenovirus by indirect immunofluorescence.19882848488
factors influencing the severity of escherichia coli and avian adenovirus group ii infections in chickens.chickens from lines selectively bred for either a high (hh) or low (ll) antibody response to sheep erythrocytes were challenged intravenously with avian adenovirus group ii (aa). spleen size was determined 6 days later. in some experiments the responses of chickens to aa and escherichia coli infections were compared. the level of corticosterone in the feed (15 mg/kg) which resulted in the lowest incidence of pericarditis in response to e. coli resulted in the greatest incidence of large spleens ...19882849405
[avian celo adenovirus as a possible contaminant in cell cultures of japanese quail embryos].avian adenovirus celo was found to replicate poorly in japanese quail embryos (jqe) and cell cultures of them. the infectious process in these systems was latent. the antigen of adenovirus celo in jqe cell culture was detectable by the fluorescent antibody method (fam) within the first 24-72 hours after inoculation as fluorescent cytoplasmic granules. subsequently, fluorescence of nuclei and macrophage cytoplasm was observed. the results indicate that jqe and their cell cultures are not contamin ...19882854677
viral agents associated with outbreaks of diarrhea in turkey flocks in quebec.the relative importance of various enteric viruses associated with diarrhea of turkey poults was investigated by an evaluation of specimens received since 1982. specimens originated from one to eight week old turkey poults, with mild to severe diarrhea, from 114 flocks in 42 commercial operations located in southern quebec. the acute phase of enteritis occurred usually in poults between two and four weeks of age. clarified intestinal contents were examined by direct electron microscopy and enzym ...19882832044
characterization of group ii avian adenoviruses with a panel of monoclonal antibodies.the interaction between a panel of ten monoclonal antibodies and hemorrhagic enteritis virus, a group ii avian adenovirus, was determined. the monoclonal antibodies reacted with all nine isolates of group ii avian adenoviruses, but not with any of five types of group i avian adenoviruses. all ten monoclonal antibodies recognized antigenic determinants on the hexon protein of hemorrhagic enteritis virus when analyzed by immunoprecipitation and immunoblotting. they reacted only with the native hex ...19882461793
effect of adrenal blocking chemicals on viral and respiratory infections of chickens.in a series of experiments chickens were treated with chemicals which block the production of corticosterone by the adrenal cortex prior to being challenged with respiratory disease (and other) agents in order to determine if the course of the diseases could be altered. some chickens received a single intramuscular injection (14 mg/kg) of 1,1-dichloro-2,2-bis/p-chlorophenyl/ethane (abc) dissolved in corn oil (20 mg/ml) at least 12 h before challenge. other chickens received feed containing 500 m ...19892536580
detection of latent avian adenovirus-associated virus proteins in chicken cells.chicken kidney cells, derived from the eggs of white leghorn chickens that had serological evidence of prior exposure to both adenovirus and the adeno-associated virus (aav), produced aav antigenic proteins upon challenge with purified adenovirus. antigen was detected by indirect immunofluorescence using monoclonal antibody to aav. the number of fluorescent cells were few and did not increase during the course of adenovirus infection. similar results were obtained using cells prepared from speci ...19892539071
vaccine for the control of egg drop syndrome '76. 19892543116
dna sequence analysis of the inverted terminal repeats of a non-oncogenic avian adenovirus. 19892543962
in situ hybridization: an optimised detection protocol for a biotinylated dna probe renders it more sensitive than a comparable 35s-labelled probe.the development of a highly sensitive biotinylated in situ hybridization protocol using egg drop syndrome virus infection of domestic fowl as a model is described. this protocol incorporates the use of a monoclonal antibody to biotin as the initial step in a peroxidase-based detection system. the detection of viral nucleic acid in formalin-fixed, paraffin-embedded sections using biotinylated and 35s-labelled probes is compared to the detection of viral antigen by immunocytochemistry. biotinylate ...19892547814
observations of experimentally induced quail bronchitis.quail bronchitis was experimentally reproduced in captive-reared bobwhite quails (colinus virginianus). quails were inoculated with 10(6) mean tissue culture infective doses of quail bronchitis virus at 1,3,6, or 9 weeks of age by the intratracheal, intraperitoneal, or subcutaneous route. clinical signs were minimal, but occasionally birds were ruffled, exhibited open-mouthed breathing, and developed "snicks." mortality rates of quails inoculated at 1 or 3 weeks ranged from 7% to 87%. quails ino ...19892549936
adenoviral pancreatitis in guinea fowl (numida meleagris).necrotizing pancreatitis was observed in 2-week-old guinea fowl submitted for necropsy and histopathology. intranuclear inclusion bodies seen histologically in acinar epithelium were examined by electron microscopy and found to contain viruses resembling adenovirus. adenovirus was isolated in embryonated eggs from the pancreata of affected birds. the adenovirus isolated was not neutralized by chicken antisera developed against 10 known serotypes of group 1 avian adenoviruses.19892549945
differentiation of avian adenovirus type-ii strains by restriction endonuclease fingerprinting.three serologically indistinguishable viruses from the avian adenovirus type-ii splenomegaly virus of chickens, marble spleen disease virus of pheasants, and hemorrhagic enteritis virus of turkeys, were analyzed by restriction endonuclease fingerprinting. the dna from these viruses were examined with 6 restriction endonucleases (bgl ii, ecori, hindiii, hha i, xho i, and bamhi). markedly different dna cleavage patterns were found in these virus isolates with all the 5 enzymes, except with bamhi, ...19892552877
a review of marble spleen disease of ring-necked pheasants.marble spleen disease (msd) is a contagious disease of captive-reared ring-necked pheasants (phasianus colchicus) characterized by variable mortality and high morbidity. the etiologic agent is a type ii avian adenovirus closely related to hemorrhagic enteritis virus of turkeys and splenomegaly virus of chickens. gross lesions of msd consist of enlargement and mottling of the spleen and pulmonary edema. microscopic alterations are most pronounced in the spleen and are characterized by reticuloend ...19892553999
[immunoenzyme analysis of adenovirus hexons. indication and characteristics of rabbit and mouse antibodies against hexons].an enzyme-linked immunosorbent assay (elisa) was developed for analysis of rabbit and mouse igg antibodies specific to adenoviral hexon. the anti-hexon antibodies were detected by capture with purified hexon coated onto polystyrene microtiter plates and visualizing them by respective anti-igg horseradish peroxidase conjugates. in the sera from hyperimmunized rabbits and mice as well as in the mouse ascite fluids the elisa procedure revealed primarily type-specific (epsilon) and genus-specific (a ...19892554131
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