Publications

a comparative study of marek's disease virus proteins.		1973	4360181
biological and biochemical evidence for an interaction between marek's disease herpesvirus and avian leukosis virus in vivo.	the dna-containing epstein-barr herpesvirus has been implicated in the etiology of burkitt's lymphoma, a malignant tumor of children in africa. recently, however, particles possessing four biochemical characteristics of rna tumor viruses have also been identified in these tumors. the fact that both types of viruses are found suggests that an interaction between them may be playing a role in the etiology of burkitt's lymphoma. to explore this possibility with a defined animal model, experiments w ...	1973	4361680
membrane antigens in arginine-deprived cultues infected with marek's disease herpesvirus.		1973	4519604
ultrastructure of tumor cells in marek's disease virus-infected chickens.		1973	4543201
susceptibility of mammalian (hamster) cell culture to infection with cellular and cell-free type ii leukosis (marek's disease) virus.		1973	4348217
detecting marek's disease herpesvirus: evaluation of biological systems.		1973	4197288
immunoglobulin response in experimental infection with cell-free and cell-associated marek's disease virus.		1973	4123370
[comparison of the effectiveness of marek's disease virus isolation from lymphocytes and spleen cells in chickens].		1974	4210142
comparison of antigenic glycoproteins and glycoprotein receptors of concanavalin a isolated from duck embryo cells infected with marek's disease virus and a herpes virus of turkeys (strain fc126).		1974	4214897
properties of the common antigen associated with marek's disease herpesvirus and turkey herpesvirus infections.		1974	4363692
replication of marek's disease virus in cell cultures derived from genetically resistant chickens.	various parameters of replication of marek's disease virus (mdv) were studied in cells derived from genetically resistant (line 6) and susceptible (line 7) chickens. cell-free and cell-associated preparations of three strains of pathogenic mdv (jm, ga, and id-1) replicated equally well in cells of resistant and susceptible chickens. there were no demonstrable differences between the two genetic sources of cells for the chronological appearance and type of cytopathic effects and for the type of v ...	1974	4364506
responses of isolator-derived and conventional chickens to marek's disease herpesvirus and avian leukosis virus.		1974	4364733
marek's disease virus and the herpesvirus of turkeys.		1974	4371178
processing marek's disease vaccine.		1974	4376435
[morphogenesis of marek's disease virus and turkey herpes virus].		1974	4377357
[marek's disease virus and turkey herpes virus].		1974	4377364
[evaluation of membrane antigen seen in marek's disease virus-infected cells in the arginine-deficient medium].		1974	4377365
a transplantable lymphoma induced with marek's disease virus.		1974	4411786
initial lesions in chickens infected with jm strain of marek's disease virus.		1974	4420956
[isolation of marek's disease virus ("concours-1" strain) and the results of its inoculation into chicks].		1974	4439829
marek's disease herpesvirus-induced dna polymerase.	infection of duck embryo fibroblasts by marek's disease herpesvirus (mdhv), strain ga, led to the induction of a novel dna polymerase. this novel dna polymerase, designated mdhv-induced dna polymerase, could be distinguished from the dna polymerase activities of uninfected duck embryo fibroblasts by its chromatographic behavior on phosphocellulose, by its sedimentation coefficient, and by its catalytic properties. the characteristics of mdhv-induced dna polymerase which had been purified by phos ...	1974	4473569
deoxyribonucleic acid of marek's disease virus in a lymphoblastoid cell line from marek's disease tumours.		1974	4474358
transient infectivity and heterokaryon formation in hamster cell cultures inoculated with cell-associated stocks of marek's disease virus and herpesvirus of turkeys.		1974	4612166
[isolation and passaging of marek's disease virus in cell cultures].		1974	4614587
high-virulence marek's disease virus infection in chickens previously infected with low-virulence virus.		1974	4831444
the in vitro and in vivo effect of chemotherapeutic agents on the marek's disease herpesvirus of chickens.		1974	4854877
biogenesis of marek's disease (type ii leukosis) virus in vitro: electron microscopy and immunological study.	the kinetic events involved in marek's disease herpesvirus infection of avian cell culture were investigated by assaying viral infectivity and antigenicity as well as by electron microscopy during the infectious cycle. the levels of viral infectivity and complement-fixing (cf) antigens revealed that the rates of appearance of infectious particles and cf antigens were not synchronous. viral specific cf antigen could be detected 5 h after infection, whereas viral infectivity or the appearance of v ...	1974	4856684
dna configuration in the core of marek's disease virus.	marek's disease virus dna appears to be wound around a central structure connecting the two inner poles of the capsid. based on electron micrographs of marek's disease virions, a diagram of spatial configuration of virus dna is presented. this diagram may explain the pleomorphic character of the herpesvirus core observed with the electron microscope.	1974	4856840
ultrastructure of lymphoid tissue from chicks infected with marek's disease virus.		1974	4857027
a study of vaccination against marek's disease with an attenuated marek's disease virus.	approximately 2,000 day-old white leghorn chickens were distributed into 10 pens and half the pens vaccinated with the attenuated strain of hprs-16. at 72 weeks of age, mortality from non-specific death and marek's disease was 17.6% and 15.6% respectively, in vaccinated chickens compared with 14.2% and 51.7% in unvaccinated chickens. body weights of vaccinated chickens were 5.6% higher at 8 weeks of age than unvaccinated chickens. vaccinated chickens consumed 2.3% more feed and the hen housed eg ...	1974	18777267
comparative features of low-virulence and high-virulence marek's disease virus infections.	virus clones from 2 marek's disease virus (mdv) isolates from flocks with low natural incidences of marek's disease (md) were, at most, only mildly pathogenic compared to clones from the highly virulent jm isolate. clones from a single donor bird appeared to vary in pathogenicity. the infectivity and pathogenicity of cu-2 (low virulence) and jm-10 (high virulence) were compared with age, genetic constitution and virus dosage as variables. in jm-10 infection, s-strain birds had only neural and go ...	1974	18777279
efficacy of marek's disease vaccines: protection and viraemia studies with turkey herpes virus vaccines.	two freeze dried turkey herpes virus vaccines a and c, of similar virus content were administered in graded doses to groups of day old chicks which were genetically susceptible to marek's disease. immediately after vaccination the chicks were challenged by contact with 4-weeks-old birds that had been infected at one day of age with virulent marek's disease virus strain hprs 16. assessment of protection against marek's disease was based on the absence of gross lesions after a 15 week observation ...	1975	18777298
differentiation between strains of marek's disease virus and turkey herpesvirus by immunofluorescence assays.	two serological types of marek's disease virus and a herpesvirus of turkeys have been differentiated by indirect immunofluorescence tests as (1) pathogenic strains of marek's disease virus (mdv) and their attenuated variants: hprs-16, hprs-16/att, hprs-b14, jm, jm/att, ga, vc and 'oldenburg', a recent field isolate; (2) apathogenic strains hprs-24 and hprs-27 of mdv; (3) herpesvirus of turkeys strain fc126 and its hvt(a-) variant. virus strains could not be distinguished on the basis of qualitat ...	1975	18777301
precipitating antigens associated with marek's disease viruses and a herpesvirus of turkeys.	precipitating antigens associated with a number of marek's disease virus strains and with a turkey herpesvirus have been analyzed. the 'a' antigen has been defined as the major soluble antigen in feather follicles of infected chickens, which is identical with the major antigen usually present in supernatants of chicken kidney cell cultures infected with strains of marek's disease virus. 'bc' antigens are 2 or more antigens which are usually not noted in skin extracts but present in cultured cell ...	1975	18777302
effect of maternal immunity against development of marek's disease and protective ability of vaccine.	experiments were conducted to determine the susceptibility of chicks to marek's disease and the protective effect of the vaccine prepared from herpesvirus of turkey (hvt) on progeny from dams infected with marek's disease virus (mdv), or hvt, or both when they were young. chicks from dams infected with mdv or hvt-mdv were protected from the development of marek's disease when challenged at 3 days of age. the degree of protection decreased when the time of challenge was delayed; no protection was ...	1975	804667
morphogenesis of marek's disease virus in feather follicle epithelium.	three evaluative systems, immunodiffusion, fluorescent antibody (fa), and electron microscopy (em), were used to follow the morphogenesis of marek's disease virus in inoculated chickens. of the three, em and fa were the most sensitive in detecting early stages of infection. virus particles were found in skin biopsy specimens as early as 12 days post inoculation. immature naked particles appeared first in the nucleus; later particles were enveloped in the cytoplasm and enclosed in cytoplasmic inc ...	1975	808638
properties of a chicken lymphoblastoid cell line from marek's disease tumor.	properties of a chicken lymphoblastoid cell line (msb-1) from a marek's disease tumor were studied. the cell line grew well at 41 degrees c in medium rpmi-1640 supplemented with 10% bovine fetal serum and had a doubling time of 8-12 hours. cells grown in stationary suspension culture did not attach to the vessel and had the morphology of typical lymphoblasts. at 37 degrees c, the cell line grew initially but ceased to divide after several subcultures. in the subcultures maintained for 48-72 hour ...	1975	163334
studies on the pathogenicity of high- and low-passages of a marek's disease virus (cprl ii).		1975	163469
natural pox and herpes as a dual virus infection in chickens.	a natural dual viral infection was confirmed in chickens of university of illinois poultry farm by histopathologic, immunologic, and electron-microscopic examination of formalin-fixed tracheal tissue. histopathologic examination of the tracheal mucosa revealed eosinophilic cytoplasmic inclusions characteristic of fowlpox and intranuclear inclusions suggestive of marek's disease. treatment of the formalin-fixed tracheal tissue with peroxidase and fluorescent-labeled antibody against marek's disea ...	1975	164178
in ovo assay for marek's disease virus and turkey herpesvirus.	marek's disease virus (mdv) and the turkey herpesvirus (hvt) may be assayed on the chorioallantoic membrane (cam) of the chicken embryo after intravenous inoculation of chicken embryo fibroblasts (cef) or chicken blood leukocytes infected with these viruses. free hvt, mdv associated with marek's tumor cells, and lymphoblastoid cell lines derived from marek's tumors, may be assayed in the same way. the intravenous assay is quicker than the yolk sac assay and somewhat more sensitive than in vitro ...	1975	164413
pathogenic and serologic studies of japanese quail infected with jm strain of marek's disease herpesvirus.	japanese quail were inoculated with marek's disease herpesvirus at 1 day of age, and a second group of the same age was infected by contact with inoculated quail. a third group raised in our laboratory as a source of hatching eggs was accidentally infected with marek's disease (md). all quail were observed and examined for periods up to 360 days; the usual clinical signs of md were not observed. however, the birds were susceptible to md infection because of the evidence that: 1) the md-specific ...	1975	164560
antibody response of genetically susceptible and resistant chickens to cell-free and attenuated jm-v leukosis strain and its influence on early type ii (marek's) leukosis infection.	experiments were carried out to: 1) determine the antibody response of chickens to cell-free and attenuated preparations of jmv leukosis strain, 2) determine the differences in antibody response to these antigens between susceptible (p-line) and resistant (n-line) chickens by means of serum neutralization and indirect fluorescent antibody tests, 3) investigate the influence of maternal (passive) antibody on early (day-old) jmv vaccination and 4) investigate the influence of maternal antibody in ...	1975	166367
vertebrate dna polymerases.		1975	166759
newer knowledge in comparative virology--its contribution to human health research.	like other comparative sciences, and despite its recent beginning comparative virology has already contributed useful applications and observations to human health research. teachings derived from the study of marek's disease found application in that of burkitt's lymphoma, and may lead to a possible vaccine against the human disease. equally useful information came from the study of canine distemper in the development of a chorio-allantoic membrane attenuated measles vaccine, and in our knowled ...	1975	167504
studies of marek's disease herpesvirus and turkey herpesvirus specific common antigen which stimulates the production of neutralizing antibodies.	an agar-gel precipitation antigen prepared from the skin (feather-ag) of chicken infected with jm strain of marek's disease herpesvirus (mdhv) and cell extracts of cultures infected with either type 2 plaque producing agent (ppa) of mdhv or turkey herpesvirus (hvt) (type 2-ag, hvt-ag) had 1 precipitation line in common. hyperimmune sera to the common antigen (common-ag) neutralized to a greater extent by homologous antiserum. absorption of hvt antiserum or hyperimmune serum to type 2 ppa with co ...	1975	167687
detection of viral cores having toroid structures in eight herpes viruses.		1975	167709
depression of in vitro responsiveness to phytohemagglutinin in spleen cells cultured from chickens with marek's disease.	cultures of dispersed spleen cells, prepared from mdv-infected chickens with md visceral lymphomas, showed marked depression of responsiveness to the t cell mitogen pha, as measured by 3h-tdr incorporation in cells in vitro. when data are expressed quantitatively in terms of cpm/10(5) viable cells, the functional depletion of pha-responsive cells appear to result from lower levels of 3h-tdr incorporation in the pha-stimulated spleen cultures from chickens with acute md symptoms, as compared to s ...	1975	168267
trials with marek's disease vaccines prepared from a turkey herpes virus and an attenuated marek's disease virus.	in field trials involving over 224,000 fowls in 11 different commercial flocks, three vaccines were used, namely a freeze-dried vaccine prepared from a turkey herpes virus, a cell-associated virus vaccine prepared from the same isolate and a cell-associated vaccine prepared from a strain of marek's disease virus isolated from a fowl. the mortality from marek's disease was reduced by 80 per cent to 95 per cent in birds vaccinated with the freeze-dried vaccine. cell associated vaccines gave slight ...	1975	168681
influence of vaccination with avirulent herpesvirus on subsequent infection of chickens with virulent marek's disease herpesvirus.	vaccination of chickens with turkey herpesvirus (hvt) or attenuated marek's disease herpesvirus (amdhv) blocked infection with virulent mdhv (vmdhv) for approximately 5 weeks after contact exposure. however, there was no apparent blockage of infection when challenge virus was administered intraabdominally (ia). evidence for infection with vmdhv was based on viral isolation by in vivo assay or by detecting precipitins to "a" antigen associated with virulent virus. the hvt stimulated production of ...	1975	168799
oncogenesis by marek's disease herpesvirus in chickens lacking expression of endogenous (gs, chick helper factor, rous-associated virus-o) and exogenous avian rna tumor viruses.	chickens free of exogenous avian leukosis virus (alv) infection, replicating endogenous alv (rous-associated virus-o), gs antigen, and chick helper factor were fully susceptible to induction of marek's disease (md) by alv-free md viruses. dual infection with rous-associated virus-2 and md virus did not significantly alter the character of the md lesions. thus exogenous alv infection was not requisite for md virus-induced oncogenesis. although participation of endogenous rna tumor virus genes in ...	1975	169364
experiments to vaccinate marmoset monkeys against malignant lymphoma.	humoral antibodies can be induced in cotton topped (ct) marmosets with killed herpesvirus saimiri (hvs) vaccines. the serum antibodies delayed, but did not prevent, malignant lymphoma in actively and passively immunized monkeys after inoculation with 10(3.8)tcid50 of cell-free hvs. experiments are in progress to determine whether the vaccines are able to protect against smaller challenge dosages of hvs. the immunized monkeys were not resistant against transplantation of tumor cells. infection wi ...	1975	169814
continuous cell culture from lymphoma of marek's disease.		1975	169828
proceedings: immunization against marek's disease using virus specific antigens free from infectious virus.		1975	169863
serological relationship between a pathogenic strain of marek's disease virus, its attenuated derivative and herpes virus of turkeys.	precipitating antigens present in extracts of chick embryo cells infected with the hprs-16 attenuated strain of marek's disease virus (att-mdv) were separated by gel filtration on sephadex g200 and some of their properties determined. the two main antigens detected with convalescent md serum, referred to as 'b' and 'c' antigens, had mobilities of 0-55 and 0-25 respectively relative to phenol red on electrophoresis in 7-5% acrylamide gel. the b antigen was relatively stable and of low mol. wt. in ...	1975	51042
characterization of a new serotype of marek's disease herpesvirus.	the hprs-24 strain of marek's disease herpesvirus was selected for closer study from a group of virus isolates that appeared apathogenic under standard test conditons. protection studies revealed an immunological relationship between this virus strain and acute marek's disease herpesvirus. in chicken kidney cell cultures, the hprs-24 strain caused small and slowly developing plaques, and the proportion of infected cells was less than with other strains. in chicken embryo fibroblast cultures, thi ...	1975	66180
isolation of virus-induced antigens from cells infected with marek's disease and turkey herpesviruses.		1975	66181
t-lymphoblastoid cell lines from marek's disease lymphomas.	the establishment and continuous culture of two lymphoblastoid cell lines derived from marek's disease lymphomas is described. although the cells carried t-lymphocyte surface antigens, they had many features in common with cultured burkitt's lymphoma lymphoblasts, which carry b-cell determinants. a small proportion acted as infectious units in tissue culture, and a similarly small proportion contained intranuclear immature herpesvirus particles. the cells did not respond to phytohaemagglutinin. ...	1975	66182
immunization against marek's disease using marek's disease virus-specific antigens free from infectious virus.	immunity against marek's disease was conferred by the use of non-infectious materials extracted with non-ionic detergents from cells infected with the attenuated strain of marek's disease virus (mdv). antibody-free rhode island red chicks were inoculated at 1 week of age with cell extracts emulsified in freund's complete adjuvant and were given a second inoculation 1 week later without adjuvant. protection against natural infection was obtained in groups inoculated with both soluble (not sedimen ...	1975	170213
isolation of marek's disease virus from affected chickens.		1975	170571
marek's disease--the disease and its prevention by vaccination.	marek's disease (md) is a common lymphoproliferative disease of the domestic chicken caused by a cell associated herpesvirus. vaccines used for the prophylaxis of md have been derived from non-pathogenic field marek's disease virus (mdv), pathogenic mdv and the herpesvirus of turkeys (hvt). vaccines derived from mdv have been developed by attenuation of virus strains which produce the acute form of md and virus strains which produce the classical form. they have also been developed by suitable m ...	1975	170953
[testing of the protective effect of cell-free lyophilized vaccine against marek's disease in field trials].	the protective effect of a lyophilized vaccine against marek's disease ("keramvac"--pfizer), prepared from a cell-free turkey's herpesvirus (strain fc 126), was compared in field trials with losses in the group of non-vaccinated chickens. under the indicated conditions, the vaccine had only a 50.86% effectiveness. the possible causes of the reduced vaccination effect are discussed with regard to the pathomorphological and virological findings suggesting, among others, an increased incidence of t ...	1975	171821
effects of viruses on immune response.		1975	171931
immunoglobulins and anti-marek's disease virus antibody synthesis in chickens after passive immunization with immunoglobulin y anti-marek's disease virus antibody.	the effect of passive immunization with immunoglobulin y (igy) antibody against marek's disease virus (mdv) was examined in mdv-susceptible chickens. the production of igy, immunoglobulin m, and probably also immunoglobulin a was depressed in passively immunized chickens when compared with that in mdv-exposed chickens which had not been given igy anti-mdv antibody. in passively immunized chickens, the synthesis of immunoglobulin m and igy anti-mdv antibodies in response to mdv infection also was ...	1975	171985
effect of cyclophosphamide on the response of chickens to a virulent strain of marek's disease virus.	the effect of cyclophosphamide on the pathogenesis of marek's disease was examined in a line of chickens which is relatively resistant to marek's disease. the injection of cyclophosphamide into newly hatched chickens delayed and reduced viremia and also reduced the development of marek's disease lesions until 2 weeks after exposure to marek's disease virus. the data indicate that a population of t cells susceptible to infection with virus and possibly viral transformation is affected by cyclopho ...	1975	172451
responses of isolator-derived japanese quail and quail cell cultures to selected animal viruses.	thirteen oncogenic and necrotizing animal viruses were assayed in life sciences, inc. (lsi)-specific pathogen-free japanese quail and lsi-specific pathogen-free chicken embryo cell cultures. nine viruses produced similar titers in the quail and chicken cell systems, whereas four viruses showed significantly higher titers in chickens. young japanese quail and chickens were inoculated with five selected avain viruses and maintained in stainless-steel isolators. comparable responses were noted in q ...	1975	172527
morphological response of maternal antibody-negative chickens infected with marek's disease virus (mdv), herpesvirus of turkeys (hvt) and both viruses.		1975	172684
enhancement and interference in chickens inoculated with marek's disease herpesvirus and oncornaviruses.	enhancement of mortality rates and symptomatology was observed in isolator-held lsi-spf chickens concurrently inoculated with mdhv and avian oncornaviruses (rav-1, rav-2, rav-7, rav-50, or rev). interference with md antigen production also was demonstrated in extracts of the feather follicle epithelium from chickens inoculated with both mdhv and rav-1.	1975	174137
[mark's disease: v. experimental behavior of 3 isolates taken place in the country].	three experiments were designed to determine the parameters of virus infection, antibodies and mortality with three different md isolates inoculated in one day old birds from commercial origin. the animals were divided in three inoculated lots (1-2-3) and three control groups (4-1, 4-2, 4-3) and were followed weekly from hatching through 17 weeks. the former were inoculated respectivelly with fov-6, fcv-8 and fcv-9. each day old bird received between 50-75 fpu/bird by i.p. route. samples were ta ...	1975	174161
testing veterinary vaccines.		1975	174269
[pathogenesis of neural lesions in marek's disease. ii. transmission of neural lesions by spleen cells from ill donors (author's transl) ].	viable spleen lymphocytes of 13 chickens with clinical and histological signs of marek's disease were transferred by intravenous injection to 46 chickens 4-10 days old, x-rayed 24 hours before inoculation. histologically marek-like neural lesions were almost regularly observed in the chickens after 6-14 days. chickens having received destroyed spleen lymphocytes of marek-donors were free of nerve infiltrations. there were also no neural lesions detectable within 14 days after application of path ...	1975	175595
a transovarian transmission study of marek's disease in japanese quail.	experiments were conducted to determine if transovarian transmission of the jm strain of marek's disease virus (mdv) occurs in japanese quail. in one experiment, parent quail were infected with mdv when one-day-old; in another experiment, parent quail were infected when seven weeks old. no precipitating antigen or cytopathic or infective agent was isolated from the egg yolks, embryos, newly hatched quail, or progeny quail of the infected parents. during the eight weeks observation period, the pr ...	1975	180501
marek's disease in japanese quail -- a pathological, virological and serological study.	experimental studies were performed to see whether japanese quail (coturnix coturnix japonica) are susceptible to jm strain of marek's disease virus (mdv). in three identical trials, a total of 120, one-day-old quail were inoculated intra-abdominally with 0.2 ml. of chicken blood infected with mdv (jm strain) and raised in fapp isolators. uninfected controls were inoculated with normal saline only and raised separately. during the 18-week post infection observation period, 65-80% of the infecte ...	1975	180503
marek's disease: epizootiology, control and relationship to oncornaviruses.		1975	183670
interaction of marek's disease virus with host cell.		1975	183685
influence of some physical factors on survival of marek's disease vaccine virus.	cell-associated marek's disease (md) vaccine was suspended at dilutions normally used for vaccination in seven commercially available diluents and in tryptose phosphate broth. the stability of diluted vaccines was determined by assay in cell cultures subjected to 0 to 37 c for 0 to 90 minutes. optimum holding temperatures for md vaccine virus survival varied with the specific diluents employed. some diluents afforded greatest survival when dilution was at 0 c and held at 0 c, while others perfor ...	1975	987
induction of an early antigen of marek's disease virus in a lymphoblastoid cell line.		1975	190138
transformation in vitro by herpesviruses - a review.		1975	190141
properties of the dna polymerase induced by marek's disease herpesvirus, strain ga, upon infection of duck embryo fibroblasts.		1975	190161
lymphoid cell lines from lymphomas of marek's disease.		1975	191350
experimental evidence for influence of type-c viruses on marek's disease virus-associated oncogenesis.		1975	191359
marek's disease virus dna in a chicken lymphoblastoid cell line (msb-1) and in virus-induced tumours.		1975	191363
vaccination against oncogenic herpesviruses - a review.		1975	191377
the effect of b-cell immunosuppression on age-related resistance of chickens to marek's disease.	chickens were bursectomized by cyclophosphamide treatment at hatching. at 8 or 9 weeks of age, bursectomized and unbursectomized hatchmates, free from prior infection, were challenged with pathogenic marek's disease virus. oncogenicity of the virus inoculum was confirmed by inoculating 1-day-old susceptible chickens. at the time of virus challenge, blood cells from the cyclophosphamide-treated chickens were able to mount a vigorous graft-versus-host reaction in allogeneic embryos. this ability i ...	1975	234791
marek's disease virus (kekava strain) replication in chickens, chick embryos and cell cultures.	in the course of 12 passages of marek's disease virus (mdv) strain kekava (mdv-kekava) in chickens, the morbidity varied greatly (from 23 to 50 percent). mdv-kekava produced plaques in cultures of chick embryo kidney and adult chicken kidney cells and chick embryo fibroblasts (cef). the virus adaptation to the cultures was very slow. mdv-kekava induced the formation of pocks on the chorioallantoic membranes (cam) of chick embryos but the proportion of embryos with cam lesions did not exceed 24 p ...	1975	241226
detection of t-cell surface antigens in a marek's disease lymphoblastoid cell line.	indirect membrane immunofluorescence was applied in the study of a lymphoblastoid cell line derived from marek's disease lymphoma. the cell line tacked surface immunoglobulins and reacted specifically with antisera prepared against chicken thymus cells. cells transformed by marek's disease virus were of thymus origin.	1975	1089795
virologic and serologic studies of zoo birds for marek's disease virus infection.	one hundred and eleven zoo birds representing 49 species in 14 orders were examined for marek's disease (md) herpesvirus (mdhv) infection. mdhv was isolated from 10 birds, all belonging to genus gallus. the precipitating antibodies against mdhv were demonstrated only in the gallus birds, when 51 selected birds including 34 galliformes and 17 other birds representing 12 species from nine orders were examined. the 10 mdhv isolates all induced morphologically similar plaques in cell cultures closel ...	1975	1091554
viremic responses of genetically susceptible and resistant chickens to experimental infection with acute, mild, or both strains of marek's disease herpesvirus.	the development and persistence of viremia were followed in two lines of single-comb white leghorns: one experimental line (wsu-vs) highly susceptible, and one commercial line (c-wl) relatively resistant to marek's disease(md). in the resistant c-wl chicken, viremia with a mild strain of md herpesvirus (mdhv) persisted in all viremic birds through 8 weeks postinoculation (pi), while viremia with an acute strain of mdhv did not, resulting in a decrease in number of viremic birds after 2 weeks pi. ...	1975	1120039
a comparison of the efficacy against marek's disease of cell-free and cell-associated turkey herpesvirus vaccine.	one-day-old white leghorn and broiler chicks with maternal antibody to turkey herpesvirus (hvt) were vaccinated with 300 or 1,000 plaque-forming units (pfu) of cell-free or cell-associated hvt vaccine and challenged with virulent marek's disease virus (mdv) by contact exposure. broiler chicks receiving 300 pfu of cell-associated hvt had a 3.3% incidence of md lesions, whereas only 2.0% of those receiving 1,000 pfu had macroscopic lesions. broiler chicks vaccinated with 300 pfu of cell-free vacci ...	1975	1164319
efficacy of lyophilized turkey herpesvirus vaccine against marek's disease in broilers.	field trials involving approximately 1,250,000 chickens vaccinated with cell-free turkey herpesvirus (hvt) vaccine and 635,000 chickens vaccinated with cell-associated hvt vaccine howed both vaccines equally effective in reducing condemnations (attributable to marek's disease) at the poultry processing plant. vaccinated chickens were progeny of hens vaccinated at 1 day of age with cell-associated hvt vaccine. the maternal antibody did not interfere with the ability of either the cell-free or cel ...	1975	1228717
marek's disease herpesviruses i. production and preliminary characterization of marek's disease herpesvirus a antigen.	a method was developed for the large-scale production of marek's disease herpesvirus a antigen in duck embryo fibroblast roller bottle cultures in quantities sufficient to permit its purification and characterization. maximum yield was obtained in serum-free culture medium harvested daily. the marek's disease herpesvirus a antigen was stable at ph 2.0 and was a glycoprotein based on its sensitivity to trypsin, specific immune co-precipitation of radioactive amino acids and glucosamine, and detec ...	1975	16789152
marek's disease herpesviruses ii. purification and further characterization of marek's disease herpesvirus a antigen.	marek's disease herpesvirus a antigen was purified greater than 200-fold with a 24% recovery by ion exchange column chromatography, isoelectric focusing, and preparative polyacrylamide gel electrophoresis. the antigen had an isoelectric point of 6.68 +/- 0.03 in the presence of 1 m urea and 0.05% brij 35, a nonionic detergent, and approximately 6.5 in the absence of dissociating agents. when analyzed by electrophoresis on analytical polyacrylamide gels, the purified antigen migrated as a single ...	1975	16789153
synthesis of immunoglobulin and marek's disease virus antibody in susceptible and relatively resistant chickens.	the levels of igm, igy, and iga and the development of specific antibody to marek's disease virus (mdv) and sheep red blood cells (srbc) in young chickens susceptible and resistant to marek's disease were compared after exposure to mdv. no significant difference was noted in the immunoglobulin levels. however, the antibody response to mdv and srbc occurred more rapidly in susceptible birds. the initial titer of antibody to these antigens was higher. these differences in response, however, were t ...	1976	1255743
incidence of marek's disease virus infection in domestic fowl of punjab (india).		1976	1259659
effects of igy antibody on the development of marek's disease.	the effects of passive immunization with immunoglobulin y (igy) on the pathogenesis of marek's disease (md) were examined in an experimental line of white leghorn chickens highly susceptible to md. purified igy with anti-mdv antibody activity, when injected into chicks, delayed the development of mdv viremia and lesions until 9 days postinoculation (pi) with marek's disease virus (mdv). the blastogenic response of spleen cells to concanavallin-a was depressed at 6 days pi in the birds without pa ...	1976	1259663
prevention of marek's disease: a review.	marek's disease (md) is a highly infectious neoplastic condition of chickens caused by a herpesvirus. the virus is cell associated in tumors and in all organs except in the feather follicle where enveloped infectious virions egress from the body. from this source, infection is spread horizontally by the airborne route to the environment and to other chickens. vertical transmission from dam to offspring does not occur or at best is very rare. the nonpathogenic herpesvirus of turkeys (htv) is ubiq ...	1976	766959
preservation of a t cell-mediated immune response in marek's disease virus-infected chickens by vaccination with a related virus.		1976	774984
sequential changes in the numbers of b and t lymphocytes and other leukocytes in the blood in marek's disease.	numbers of b, t and total lymphocytes, monocytes, heterophils, eosinophils and basophils have been examined in the peripheral blood of chickens between 2 and 42 days after infection with marek's disease virus. during the stage of the acute restrictively productive virus infection of lymphoid tissues at 2-9 days after infection, absolute numbers of b cells, t cells, total lymphocytes and heterophils were increased, those of monocytes and eosinophils were decreased, and those of basophils were unc ...	1976	789260
mechanism of phosphonoacetate inhibition of herpesvirus-induced dna polymerase.	phosphonoacetate was an effective inhibitor of both the marek's disease herpesvirus- and the herpesvirus of turkey-induced dna polymerase. using the herpesvirus of turkey-induced dna polymerase, phosphonoacetate inhibition studies for the dna polymerization reaction and for the deoxyribonucleoside triphosphate-pyrophosphate exchange reaction were carried out. the results demonstrated that phosphonoacetate inhibited the polymerase by interacting with it at the pyrophosphate binding site to create ...	1976	55273
relation between common antigen and membrane antigens associated with marek's disease herpesvirus and turkey herpesvirus infections.	the appearance of two kinds of membrane antigen (ma) in marek's disease herpesvirus (mdhv) or herpesvirus of turkey (hvt) infected cells was examined using antiserum to the common antigen (common-ag). no early-appearing ma(ema) was detected in these cultures until 16 hours post-inoculation, after which a number of cells in these cultures had late-appearing membrane antigen (lma) as detected by immunofluorescence (if). fluids from cultures infected with these viruses were examined for the presenc ...	1976	58652
[comparative antigenic characteristics of 2 marek's disease virus variants and of herpesvirus of turkeys isolated in the ussr].	the antigens induced by pathogenic (55) and nonpathogenic (83) variants of marek's disease virus, the kekava strain (mdv-kekava) and herpesvirus of turkeys, the m-24-72 strain (hvt-m24-72) were tested in the agar gel precipitation test. the antigens were prepared from feather follicles (ff) of the infected chickens, from the infected cultures of chick embryo fibroblasts (cef) and from the culture fluid (cf) of these culttures. in the ff and cef cultures infected with mdv-kekava-83, the synthesis ...	1976	64031