Publications

intradermal skin tests with dermatophagoides pteronyssinus in asthmatic children: correlation with specific ige and bronchial provocation tests.	the purpose of the present study was to compare the results of the skin tests, the specific ige levels and bronchial provocation tests in a group of sensitive asthmatic children with a dermatophagoides pteronyssinus extract standardized by the rast inhibition method. skin tests showed a positive 'end point' in twelve children of 0.5 u/ml antigen; in eleven 5 u/ml; seven of 50 u/ml; six of 500 u/ml. specific ige was present in thirty-three children (92%). a close relationship between positive ski ...	1979	498488
[characteristics of an allergen from dermatophagoides pteronyssinus mites].	the laboratory cultivation of house dust mites (dermatophagoides pteronyssinus) was shown to yield the maximum amount of the mites in 10--12 weeks. hair accumulated in electric razors and house dust mixed with hair from electric razors should be considered the most promising medium for their cultivation. scarification tests with d. pteronyssinus allergen prepared with the use of specially developed technology produced positive results in 56% of patients hypersensitive to house dust allergen.	1979	494900
mite allergen content in commercial extracts and in bed dust determined by radioallergosorbent tests.	radioallergosorbent (rast) direct binding and inhibition type assays were used to quantitate the mite (dermatophagoides pteronyssinus) allergen content of four commercial mite extracts and a laboratory prepared extract from freeze-dried mites. the content of mite allergen in extracts prepared from twenty samples of dust vacuumed from bedding was measured by rast inhibition assay. the four commercial mite extracts designated a, b, c and d, and the laboratory extract, designated l, contained 52, 2 ...	1979	466752
skin sensitivity tests in nigerians with bronchial asthma.	skin sensitivity tests using the prick method were performed on 174 patients with bronchial asthma and 160 controls, using 20 common allergens. the results revealed a high incidence of positive skin test reactions to dermatophagoides pteronyssinus, grass and flower pollens, feathers, and some ingesta such as cow's milk and fish. the difference in the reactions between the asthmatic patients and the controls was statistically significant. the results suggest that house-dust mite allergy is the mo ...	1979	473323
the role and allergenic importance of storage mites in house dust and other environments.	skin tests were performed on 210 patients with house dust allergy and bronchial asthma or perennial rhinitis using extracts of dermatophagoides pteronyssinus and of four storage mites most commonly found in house dust in the united kingdom--acarus siro, tyrophagus putrescentiae, lepidoglyphus (formerly glycyphagus) destructor and glycyphagus domesticus. the results of the skin tests were related to certain occupations and living conditions of the patients which could have exposed them to storage ...	1979	519837
the allergenic importance of house dust and storage mites in asthmatics in brunei, s.e. asia.	examination of dust from sleeping areas showed that storage mites, especially glycyphagid species, were predominant in brunei house dust. on skin testing sixty asthmatics with 1% extracts of six mite species, dermatophagoides pteronyssinus was found to provoke the greatest number of positive skin reactions (66.7%), but positive reactions for tyrophagus putrescentiae (50%), acarus siro (35%), glycyphagus domesticus (40%), and lepidoglyphus destructor (45%) demonstrated that storage mites are also ...	1980	7438419
some effects of microclimate on the longevity and development of dermatophagoides pteronyssinus (trouessart).		1980	7211146
[protective effect of ketotifen and disodium cromoglycate in bronchial challenge tests with allergens (author's transl)].	the protective effect of ketotifen and disodium cromoglycate (dscg) was evaluated by a randomised double blind cross-over study in 15 children with allergic bronchial asthma. 13 children were allergic to house dust mite (dermatophagoides pteronyssinus), two to grass pollen. after a positive bronchial challenge test ketotifen or dscg was given for 4 days followed by bronchial challenge with increasing allergen concentrations. seven patients showed better allergen tolerance after ketotifen, 5 afte ...	1980	6780292
controlled trial of an electrostatic precipitator in childhood asthma.	a cross-over trial was conducted on 10 children with moderate to severe asthma, who had positive skin tests to dermatophagoides pteronyssinus and nocturnal wheeze. an electrostatic precipitator was used during the night to remove airborne particles from the bedroom. during use of the precipitator peak expiratory flow rates were no better than in a control period.	1980	6106739
purification and characterization of the major allergen from dermatophagoides pteronyssinus-antigen p1.		1980	6771329
effects of anti-mite measures on children with mite-sensitive asthma: a controlled trial.	mite counts and tests for mite antigen were performed on samples of dust taken from the bedding of 53 children with mite-sensitive asthma. the samples from damp houses and the beds or enuretic children had markedly more mites and mite-antigen than those from dry houses. although the predominant species was usually dermatophagoides pteronyssinus, some of the beds in the damp houses were heavily infested with another pyroglyphid mite euroglyphus maynei, so that this was the species found in the gr ...	1980	7001667
physicochemical and immunochemical characterization of the high molecular weight allergens from dermatophagoides pteronysisinus with particular reference to the tridacnin, con-a and s107 reactive components.	high molecular weight allergens which react with the s107 murine iga myeloma and with the lectins concanavalin a (con-a) and tridacnin have been identified and characterized in extracts of the mite dermatophagoides pteronyssinus. mite extract from two different manufacturers were fractionated by con-a affinity chromatography. the major antigen in both the con-a bound and unbound fractions, which was antigenically indistinguishable, reacted with tridacnin and the s107 myeloma. this high molecular ...	1980	7458776
physicochemical and immunochemical characterization of the allergens from the mite dermatophagoides pteronyssinus.	the physicochemical and immunochemical characteristics of the allergens from dermatophagoides pteronyssinus have been described, in particular those for the allergens designated dpt 4 and dpt 12. dpt 4 is a high molecular weight allergen with an apparent molecular of 274,000 daltons and a pi of 4.5. in contrast, the antigenically unrelated dpt 12 has an apparent molecular weight of 24,000 daltons and a pi 6.6. both allergens demonstrate heterogeneity, either in molecular weight or pi. dpt 12 app ...	1980	7458775
[gel filtration analysis of the protein antigens of dermatophagoides pteronyssinus ticks].	gel filtration analysis was used to study the antigenous composition and molecular weight of the culture extract from the ticks d. pteronyssinus. the highest allergenic and immunochemical activity was shown by elution fractions i and ii taken together. their activity exceeded that of the whole extract by 69%. the molecular weight of antigens entering elution fractions i and ii is about 49000--28000. fraction iii is immunochemically inert and exhibits little allergenic activity.	1980	7397361
studies on dermatophagoides pteronyssinus allergens: measurement of the relative potencies of d. pteronyssinus purified extracts by in vitro and in vivo methods.	to standardize the dermatophagoides pteronyssinus extracts used in clinical allergy practice, the relative potencies of several purified extracts were estimated by radioallergosorbent test (rast) inhibition, histamine release, and intracutaneous titration testing, and the results were compared. the potencies of nine independently prepared d. pteronyssinus extracts were assayed with respect to an extract adopted as a reference. in vivo the cutaneous wheal surface after intradermal injections was ...	1980	6154726
ascaris lumbricoides and the human immunogenic response: enhanced ige-mediated reactivity to common inhaled allergens.	the relationship between the ige-mediated immunogenic response to ascaris and similar responses to common inhaled allergens was determined. sixty-six of 170 allergic asthmatics from an area in which ascaris infestation was endemic had positive skin-prick reactions to ascaris antigen. the number and size of skin-prick reactions to common inhaled allergens were greater in this group than in the 104 ascaris-negative allergic asthmatics. specific serum ige antibodies were determined by the radio-all ...	1980	6157196
the isolation of an allergen from extracts of dermatophagoides pteronyssinus using a murine iga myeloma with a specificity for phosphorylcholine.	the presence of a high molecular weight glycoprotein component bearing the hapten phosphorylcholine (pc) has been demonstrated in some but not all extracts of house dust mite using a phosphorylcholine specific iga myeloma protein (s107). the pc bearing component was isolated from house dust mite extracts by precipitation at equivalence using whole myeloma serum and the isolated fraction was found to be allergenic as judged by a direct rast assay using a highly house dust mite allergic human seru ...	1980	6777077
standardization of allergen extracts by inhibition of rast, skin test, and chemical composition.	five allergen extracts of dermatophagoides pteronyssinus, lolium perenne, alternaria tenuis, aspergillus fumigatus and cladosporium herbarum, obtained from four different manufacturers, were examined by inhibition of rast, content of protein and carbohydrate, contents of phosphorylcholine (pc) and tridacnin reactive components, and by skin test. inhibition of rast was used as a primary method for establishing allergenic potency and demonstrated wide variations for each preparation supplied by th ...	1980	7004662
atopic allergy, serum ige and rast specific ige antibodies in patients with cystic fibrosis.	the occurrence of allergic diseases is studied in a group of 56 swiss children suffering from cystic fibrosis (cf). 27% of cf children showed allergic manifestations, and 61% of cf children had positive family history for atopic diseases. the occurrence of allergies is increased in homozygotes as well as in heterozygotes for cf. total serum ige values are clearly increased in atopic cf children, but also slightly elevated in cf patients without allergic manifestations. occurrence of specific ige ...	1980	6967864
antibody response following prolonged hyposensitization with dermatophagoides pteronyssinus extract.	an antigen-binding radioimmunoassay was used to measure igg, iga and ige antibody (ab) to dermatophagoides pteronyssinus in sera from patients who had been hyposensitized with either aqueous d. pteronyssinus extract or placebo. an allergen, f4p1, was obtained from d. pteronyssinus culture and 125i-labelled for use in the assay. the radioallergosorbent test was also used to measure ige ab to d. pteronyssinus and ige ab to dermatophagoides farinae. the radioimmunoassay results showed a selective r ...	1980	7364496
comparison of cord blood immunoglobulin e concentrations and maternal allergy for the prediction of atopic diseases in infancy.	total serum ige levels were determined in 136 newborns and their mothers and in 54 of their fathers, using the paper radioimmunosorbent test (prist) technique. ige specific antibodies for house dust (dermatophagoides pteronyssinus), orchard grass, timothy grass, and cow's milk were measured with the radioallergosorbent test (rast). one hundred thirty-three rast assays were negative in newborns, and in three cases rast for cow's milk was positive. cord blood ige ranged from 0 to 5.5 iu/ml (mean 0 ...	1980	7372964
human basophil degranulation in dermatophagoides allergies: 93 cases.	in tests conducted on 93 subjects human basophil degranulation (hbd) with dermatophagoides pteronyssinus (dp) and dermatophagoïdes farinae (df), often involved in house dust allergy, were studied. the human basophil degranulation technique involves the enrichment of the circulating basophils of the patient allowing readings in the microscope on a fixed and colored slide and a count of at least 100 basophils, the minimum number necessary to give an adequate interpretation of the results. for each ...	1980	7377643
ige antibodies to house dust mite in patients with scabies.	of 135 patients with scabies, forty-seven (35%) had a positive (classes 1--4) radioallergosorbent test (rast) to dermatophagoides pteronyssinus (dp). elevated serum ige concentrations were found in sixty-one (45%) of the patients. atopic disease was confirmed in twenty-eight (23%) of the 120 patients who completed a medical questionnaire satisfactorily. in a control group, well-matched for age and sex, of 201 employees at a local bank, only four (2%) had a positive rast to dp, and eight (4%) had ...	1980	7426424
allergen skin-prick testing in asthmatic children.	allergen skin-prick tests to the common airborne allergens were performed on 534 children with asthma. a positive reaction to one or more allergens was observed in 81%, and the prevalence increased with age. the specific allergen reactions were characteristic with a high prevalence of reactions to house-dust mite (dermatophagoides pteronyssinus) (88%), house dust (79%, and cat fur (62%), and a low prevalence of grass-pollen reactions (12% to 25%). we found that the diagnosis of atopy could be ma ...	1980	7432312
in vitro demonstration of specific immunological hypersensitivity to scabies mite.	an extract prepared from 200 adult female scabies mites was used for prick and intracutaneous testing in twelve patients with previous scabies infestation, as well as in six healthy controls and three persons with skin sensitivity to dermatophagoides pteronyssinus who had never had scabies before. seven individuals who had had scabies less than a year prior to the testing had positive intracutaneous (immediate type) reactions, whereas all the five who had had scabies more than a year before had ...	1980	7437302
the effect of ascorbate on cellular humoral immunity in asthmatic children.	ten white children with bronchial asthma and exercise-induced bronchoconstriction were assessed immunologically before and 1, 3 and 6 months after the commencement of standard therapy supplemented with ascorbate 1 g/d. the tests of cellular immune function were neutrophil chemotaxis, phagocytosis and resting and stimulated nitroblue tetrazolium reduction, and lymphocyte mitogen-induced transformation. humoral functions measured were secretory iga, serum immunoglobulins, alpha 1-antitrypsin, c3, ...	1980	7444701
screening for ige-mediated allergy.	sera from 425 patients were analysed with phadebas rast reagents against a panel of 19 allergens and the most commonly positive results were to grass pollen, dermatophagoides pteronyssinus and cat epithelium. of the 275 sera rast-positive to any allergen, 261 were positive by a rast technique which used a mixture of the three most common u.k. allergens; the remainder were usually positive to a mould. we conclude that 1) when a positive result is obtained after screening by the mixed-allergen ras ...	1980	7468940
allergy to castor bean in the sudan: measurement of serum ige and specific ige antibodies.	allergy to castor bean was diagnosed in thirty-nine dockworkers from port sudan, on the basis of rhinitis and/or asthma provoked by castor bean dust, together with a positive prick test to a high dilution of castor bean extract. these were compared to twelve non-allergic dockworkers from port sudan and forty-three other port sudan residents. the castor bean allergic group had significantly higher ige levels (mean 902 iu/ml) than the non-allergic dock workers (mean 245 iu/ml) or the port sudan re ...	1981	6976240
the relationship between air-borne fungal spores and dermatophagoides pteronyssinus in the house dust.	the prevalence of mould genera and their association with the house dust mite (dermatophagoides pteronyssinus) were investigated in the homes of 192 asthmatic patients and 125 healthy people in a romanian town with a relatively high degree of humidity. the dominant moulds which developed in the culture media exposed on the bedroom floors were cladosporium, penicillium and aspergillus. living dermatophagoides pt. and their eggs were present mostly in the colonies of cladosporium; in those of peni ...	1981	7233050
serum ige before and after treatment for scabies.	in 120 patients, treated for scabies infection, the serum ige concentrations fell from high levels in 54 and normal levels in 66 to lower levels 12 months after treatment. the specific ige antibodies to dermatophagoides pteronyssinus (rast) were also reduced 12 months after treatment. the decrease in both ige concentrations and ige antibodies was most conspicuous in non-atopic patients. these findings confirm previous observations of specific immunological hypersensitivity to scabies mite, and t ...	1981	7235206
the prevalence of immediate positive skin tests in nepalese children.	two hundred and ninety-three normal schoolchildren between 5 and 15 years old living in the hills of eastern nepal were tested by the skin prick method for sensitivity to six allergens: dermatophagoides pteronyssinus, aspergillus fumigatus. cladosporium herbarum, penicillium notatum, mixed pollens and mixed threshings. these children were also questioned and examined for symptoms and signs relating to allergic disease. of the children, 20% were skin-prick positive to at least one of the allergen ...	1981	7249341
correlation between dermatophagoides pteronyssinus and dermatophagoides farinae in house dust mites in chronic urticaria.		1981	7298373
the distribution of dust mite allergen in the houses of patients with asthma.	using an inhibition radioimmunoassay for the major allergen from dermatophagoides pteronyssinus (antigen p1), we studied the distribution of this dust allergen in the houses of patients with asthma. both bed and floor dust samples contained a wide range of antigen p1, 100 to 100,000 ng/g of fine dust, and this concentration correlated well with the number of mite bodies (r = 0.81, p less than 0.001). we were unable to detect antigen p1 in the air of undisturbed rooms. however, during domestic ac ...	1981	7305119
skin sensitivity reactions in nigerian children with bronchial asthma.	skin sensitivity reactions to a variety of antigens in 290 unselected urban nigerian children suffering from bronchial asthma of varying severity, are reported. the percentages of positive skin reactions to the first four antigens were as follows: ascaris (25%), dermatophagoides pteronyssinus (21%), house dust (12%) and feathers (12%). the sizes of reaction were generally smaller than those reported in european and american children. while the relatively high sensitivity to ascaris was probably ...	1981	7330921
preliminary studies on the biology of dermatophagoides pteronyssinus: life-cycle in an air-conditioned tropical laboratory.		1981	7344116
the incidence and causative factors of atopic asthma and rhinitis in an orkney farming community.	fifty farming families on the mainland (the largest island) or orkney were surveyed to assess the amount of allergic respiratory disease and the principal causative factor. this account deals with only type i allergy and demonstrates a prevalence of asthma and rhinitis of 17.3% and of extrinsic asthma and rhinitis, in which definite allergic causes can be identified, of 12.7%. in addition it is shown that over one-fifth of the farming community are 'atopic' in that they demonstrate positive pric ...	1981	7249337
wheezing bronchitis in children on a south pacific island.	retrospective and prospective studies were done on children with wheezing bronchitis on the small pacific island of niue. wheezing bronchitis was found to be a common cause of morbidity, but not mortality in these children. episodes of the disease were most common in children under the age of 4 years and tended to disappear as they became older. a case-control study indicated smoking by the mother (p less than 0.0001), positive stool examination for parasites (p less than 0.001), mother with a h ...	1981	7212169
effect of calcium antagonists on allergen-induced asthma.	the protective effect of inhaled verapamil (estimated dose 2 . 4 mg) and sub-lingual nifedipine (20 mg) against allergen-induced asthma were each assessed by the administration of calcium antagonists 30 min before bronchial provocation tests using dermatophagoides pteronyssinus extracts on eight young perennial asthmatics. neither drug changed the basal forced expiratory volume in one second (fev1) or the provocation dose of allergen required to cause a 20% fall in fev1 (pd20 . fev1). that calci ...	1982	7151269
house dust mites in bristol.	a survey of bedding in bristol revealed reducing mite concentrations on children's toys, mattresses, overblankets and pillows. dermatophagoides pteronyssinus was the commonest mite found. a vacuuming schedule was devised for removing mites so that a double blind trial could be carried out. investigation suggests that even with expert utilization of efficient vacuum cleaners the clinical efficacy would be limited as the mites appear to rapidly migrate back into the bedding.	1982	7151267
specific iga and ige in childhood asthma, eczema and food allergy.	allergen-specific iga and ige antibodies were compared in 250 children with asthma (dermatophagoides pteronyssinus, rye grass pollen), in eighty-six children with eczema (whole egg, cow's milk) and in two groups of children with egg and cow's milk allergy. in each of the conditions investigated, food allergy, asthma and eczema increasing atopy was associated with increasing specific ige levels to relevant allergens. there was no association of high ige antibody levels with low iga antibody level ...	1982	6982792
biochemical and allergenic properties of the house dust mite extract, dermatophagoides pteronyssinus.	a further study has been made on the house dust mite extract, dermatophagoides pteronyssinus, with emphasis on gel-filtrated fraction 2 (f2). the crude mite extract showed at least nine discs on polyacrylamide disc electrophoresis and contained less than 0.25% sialic acid and less than 0.5 mm hexosamine and no detectable uronic acid. from gel filtration of the crude extract a no 2 fraction (f2) with allergenic activity showed at least five components on sds disc electrophoresis covering a molecu ...	1982	6753629
isolation and characterization of the allergen dpt 12 from dermatophagoides pteronyssinus by chromatofocusing.	the allergen dpt 12, from the house dust mite dermatophagoides pteronyssinus, was isolated from spent growth medium by chromatofocusing. the allergen was isolated in the pi range 5.86-6.95, indicating heterogeneity with regard to pi. however, on sodium dodecylsulphate-polyacrylamide gel electrophoresis the allergen appeared homogeneous with an apparent molecular weight of 27,000 daltons. radioallergosorbent scores utilizing paper discs coupled with dpt 12 correlated significantly (p less than 0. ...	1982	7129660
[hypersensitivity to dermatophagoides pteronyssinus in librarians].		1982	7115095
improvement of the radioallergosorbent test (rast) sensitivity by using an antibody specific for the determinant d epsilon 2.	a technical modification was introduced by the manufacturer (pharmacia) of a commercially available rast, with the use of an anti-ige antibody specific for the domain epsilon 2 of the ige molecule. results of the previous and of the new rast were compared in 100 patients suffering from bronchial asthma or chronic rhinitis. when the same serum was assayed by both techniques, the new rast technique disclosed a higher positivity in 26% of the comparisons. the new rast improved the overall correlati ...	1982	6175440
mites living in hay: an important allergen source?	fifty-two farmers and 21 non-farmers with symptoms related to hay contact were investigated. skin prick tests (spt) and rast were performed with an extract made of barn dust (bde) consisting of hay remnants. at microscopy, the barn dust was found to contain large amounts of dead mites, most of them identified as lepidoglyphus destructor and acarus siro. twenty-one patients (9 farmers and 12 non-farmers) had positive rast or bde, with a good correlation to the case histories. other allergies, esp ...	1982	6897499
reproducibility of prick skin tests to five allergens.	thirty-five patients had prick skin tests to the common allergens candida albicans. aspergillus fumigatus, grass pollen, horse dander and dermatophagoides pteronyssinus performed on a regular basis from autumn 1973 to autumn 1975. specific ige to the same allergens (except c. albicans) was determined at the time of skin testing for the first five seasons. it was found that the position on the volar aspect of the forearm on which the test was performed did not affect the reaction. there was a sig ...	1982	7039862
purification of a murine ige-inducing antigen extracted from the house dust mite, dermatophagoides pteronyssinus.	a major antigen inducing ige in mice (dpi) was purified from a crude extract of dermatophagoides pteronyssinus (d.p.) using sephacryl s-200 gel filtration, de52 ion exchange chromatography and isoelectric focusing. this antigen is not one of the major d.p. human allergens. isoelectric focusing showed a single peak of pca activity at pi 4.7. activity followed a broader banding pattern on polyacrylamide gel electrophoresis (page). the molecular weight of dpi estimated from sephadex g-150 chromatog ...	1982	7048069
antigen provocation to the skin, nose and lung, in children with asthma; immediate and dual hypersensitivity reactions.	most (18/21) children with perennial asthma gave dual (immediate and late) responses to bronchial provocation with two of dermatophagoides pteronyssinus, and either timothy grass pollen or cat fur. most (19/21) also showed dual responses to skin prick tests, only half (11/21) gave dual responses in the nose, mainly with timothy grass pollen, and these were associated with allergic rhinitis. only two children gave dual responses in lung, skin and nose to both antigens, and only two gave immediate ...	1982	7083633
evaluation of the intradermal allergy test in normal dogs.	in 45 normal dogs, serial dilutions of different allergens were injected intradermally to determine the skin threshold concentrations. for housedust, housedust mite (dermatophagoides pteronyssinus) and human dandruff extracts these were found to be the same in the dog as in man, while those for animal dandruffs, pollens and fungi were 10 times higher in the dog. the term 'histamine reactive value' (rh) is introduced for the dog. the mean (+/-sd) was found to be 49.0 +/- 2.5 in normal dogs, using ...	1982	7089382
predicting the results of allergen bronchial challenge by simple clinical methods.	in this study, the ability to predict allergenic bronchial reactivity from non-specific bronchial reactivity, measured by free corridor-running, and the level of skin reactivity to whole rye grass pollen extract (wrgpe--lolium perene) and house-dust mite extract (hdm--dermatophagoides pteronyssinus) was examined in asthmatic children. discriminant analysis of the data showed that, for the concentration of extracts used and the conditions of bronchial challenge for both allergens, the level of sk ...	1982	7105393
[correlation between the prick test and specific ige against allergens of house dust and dermatophagoides sp].	of the techniques which are usually used for the in vitro etiologic diagnosis of determined allergic processes, it is the rast technique which is most widely used for determining specific antibodies of the ige class. on the other hand, also of interest is the quantification of total ige in these patients. we have studied 80 allergic and non allergic patients in order to evaluate the possible correlation between the prick and the rast tests applied to different allergens such house dust. dermatop ...	1982	7180792
[house-dust mites as vectors of human diseases].	the genuine european house-dust mite, dermatophagoides pteronyssinus, is the producer of the most common and most potent allergen in the house-dust in central europe. such allergens causing asthma, rhinitis, tracheitis, bronchitis and/or conjunctivitis in man may also be produced by the other species of house-dust mites such as d. farinae and euroglyphus maynei. house mites and mites of stored products rarely account for such diseases but more frequently for acarodermatitis--especially on farms. ...	1982	7184173
clinical associations with serum allergen-specific igg4 antibodies.	serum total and allergen-specific ige and igg4 antibodies, were measured in eighty-seven atopic and nineteen non-atopic asthmatics. the allergens studied were: dermatophagoides pteronyssinus, grass pollens, cat dander, dog dander, milk and egg. sixty-eight atopic asthmatics and fourteen non-atopic asthmatics were found to have allergen-specific igg4 antibodies, to at least one of the allergens tested. igg4 antibodies to milk and egg were common in both groups of asthmatics, and to animal danders ...	1982	6183023
the immune response to bee venom. comparison of the antibody response to phospholipase a2 with the response to inhalant antigens.	the antibody response to phospholipase a2 (pla2) was studied by using radiolabelled antigen-binding techniques for both igg and ige antibodies. the results are compared with results for antigen p1 from dermatophagoides pteronyssinus and for rye i from rye grass pollen. the response to pla2 differs from the response to the inhalant allergens in several ways: almost all exposed bee keepers produce igg antibodies to pla2; among the allergic bee keepers there was no quantitative correlation between ...	1982	7085123
in vitro selective expansion of allergen specific t cells from atopic patients.	peripheral blood mononuclear cells from atopic donors were stimulated in vitro with allergens (rye group i or dermatophagoides pteronyssinus). t cell lines were originated and maintained in long term culture using il-2 and periodical restimulations with allergen. the lines were antigen specific (i.e. responded to the allergen used to raise them and not to other antigens) and required that the antigen was presented by autologous cells (i.e. they were restricted). the restriction elements were pro ...	1983	6190594
[comparative evaluation of the specific activity of different types of allergens from dermatophagoides pteronyssinus mites].	the specific activity of different kinds of allergens prepared from dermatophagoid mites has been studied by the method of the indirect mast cell degranulation and the method of changing the volume of cerebral glial cells in patients with the atopic form of bronchial asthma. in cases of sensitization to mites the presence of reagin and precipitin antibodies has been revealed. allergens prepared from the mite medium and directly from mites have been found to possess almost the same specific activ ...	1983	6191478
identification of allergens in dermatophagoides pteronyssinus mite body extract by crossed radioimmunoelectrophoresis with two different rabbit antibody pools.	an extract of purified dermatophagoides pteronyssinus mite bodies was investigated by crossed radioimmunoelectrophoresis (crie), using sera from 29 mite-allergic patients. two crie series consisting of rabbit antibodies to (1) whole mite culture and to (2) purified mite bodies were run simultaneously, showing 27 and 29 precipitates, respectively. cases of major discrepancy between the two series were ascribed to absence of antibody to allergenic components and to artefactual radiostaining due to ...	1983	6828824
comparison of antigenic and allergenic composition of two partially purified extracts from dermatophagoïdes farinae and dermatophagoïdes pteronyssinus mite cultures.	dermatophagoïdes farinae (df 80d) and dermatophagoïdes pteronyssinus (dp 80d) extracts were analyzed for their antigenic and allergenic composition by means of crossed immunoelectrophoresis (cie) and crossed radioimmunoelectrophoresis (crie). by cie, 11 antigens could be numbered in df 80d (df 1 to df 11) and seven antigens in dp 80d (dp 1 to dp 7). this technique allowed us also to define antigens with common as well as specific parts for the two mite species. among the antigens of d. farinae a ...	1983	6853928
characterization of a very high density lipoprotein allergen, dpt 4, from the house dust mite dermatophagoides pteronyssinus.	the allergen dpt 4 from the house dust mite dermatophagoides pteronyssinus was shown to be a lipoprotein as judged by its sudan black b staining properties. ultracentrifugal studies confirmed this finding and showed that dpt 4 was polydisperse, although the majority of the allergen belonged to a very high density lipoprotein class. physicochemical studies using gradient polyacrylamide gel electrophoresis showed that dpt 4 had an apparent molecular weight of 244,000 daltons and a particle diamete ...	1983	6862663
non-allergenic haemolysins in grass pollens and housedust mites.	25 batches of pollen (six common grasses, maize, short ragweed) and two batches of housedust mite (dermatophagoides pteronyssinus) all contained haemolysins. the haemolysins of two grass pollens and of the housedust mites were of small mw (100-200 da) and apparently non-allergenic. both caused inflammation when injected into human skin, at doses that could be experienced naturally. the "allergic" airways diseases may not be entirely immunologically mediated.	1983	6638414
effects of diluents on skin tests.	the role of diluents and preservatives on skin tests was examined in 41 allergic patients. the effect of glycerol in intradermal tests was studied. concentrations of five percent glycerol were shown to induce a significant wheal which was not inhibited by clemastine fumarate, suggesting a direct toxic effect without the participation of histamine release. twenty five dermatophagoides pteronyssinus allergic patients were prick tested with standardized and lyophilized extracts reconstituted either ...	1983	6638615
total serum ige levels in venezuelan schoolchildren.	seventy-eight disease-free children were evaluated by prist for total serum ige in order to establish the normal range for this immunoglobulin and assess its utility, in tropical climates, such as venezuela, as a diagnostic tool for clinical allergy. seventy-eight normals were selected from a group of 1053 children, aged 7-12 years from fourteen schools in caracas. exclusion from the normal pool was based on nationality, and on historical, clinical, and/or laboratory evidence of atopic and/or in ...	1983	6640887
[domestic acari in sardinia].	in sardinia, a great mediterranean island, house dust mites allergy is very spread. this investigation was carried out to determine the species, occurrence and abundance of mites in the house dust of sensitized subjects. 55 samples of house dust were examined and the mites fauna identified. dermatophagoides pteronyssinus was the most abundant (54% of the found mites), and the most frequent mite (94.5%). two others pyroglyphid species were recovered: dermatophagoides farinae and euroglyphus mayne ...	1983	6661294
safety evaluation of thaumatin (talin protein).	thaumatin, the sweet proteinaceous extract of the arils of thaumatococcus daniellii (benth.) has been studied for its subacute toxicity in rats and dogs and its ability to produce anaphylactic antibodies following oral administration to rats and normal human subjects. thaumatin was readily digested prior to absorption in rats and no adverse effects resulted from its continuous administration to rats and dogs at dietary concentrations of 0, 0.3, 1.0 and 3.0% for 13 wk. it was not teratogenic when ...	1983	6686588
antibodies to dermatophagoides pteronyssinus allergen dpt 12 contaminating rabbit antisera to human and mouse anti-immunoglobulins.	antibodies to the allergen dpt 12 from dermatophagoides pteronyssinus have been demonstrated in commercially obtained rabbit anti-human ige, igg and iga antisera and in a commercially obtained rabbit anti-mouse igg1 antiserum using either double or triple antibody radioimmunoassays. where measurable, the titre of anti-dpt 12 antibodies varied both between different anti-isotypes and between batches of the same anti-isotype as judged by the dilution of antiserum required to bind 50% of the radiol ...	1983	6602850
sensitivity to house dust mite and grass pollen in adults. influence of the month of birth.	a comparison was made between the birth month of a control population and a sample of 240 adult patients with bronchial asthma or rhinitis and positive skin test towards house dust mite (dermatophagoides pteronyssinus). the patients were born more frequently (p less than 0.005) in the summer and autumn months than in the other seasons. a similar comparison of 336 allergic patients with a positive skin test towards grass pollen (phleum pratense) but no consistent seasonal preference in the birth ...	1983	6640888
the dose of house dust mite antigen (p1) inhaled by infants aged one month.	a survey of the habitats occupied by 12 infants of one month of age revealed that approximately 10% of their day was spent in conditions of potential exposure to the major (p1) allergen of the house dust mite, dermatophagoides pteronyssinus. a respiratory pump which reproduced the minute ventilation of an infant was placed in representative infant habitats. the p1 allergen trapped by the filter in this pump was measured as an estimate of infants' allergen intake. detectable p1 intake was only pr ...	1983	6638616
long-term falls in antibodies to dust mite and pollen allergens in patients with asthma or hay fever.	'spontaneous' improvement in, or alteration of, allergic symptoms is a common occurrence, and the immunological basis is of interest in attempts to develop effective specific therapy. in the present study we measured levels of serum antibodies to dermatophagoides pteronyssinus in patients diagnosed as having house-dust-allergic asthma up to 40 years previously. the results show a progressive fall in both igg and ige antibodies to antigen p1 and rast binding to crude d. pteronyssinus extract. by ...	1983	6627618
stimulation of alveolar macrophages in asthmatic patients after local provocation test.	dermatophagoîdes pteronyssinus allergen was instilled into the alveolar space of 7 allergic asthmatic patients during bronchoalveolar lavage. beta-glucuronidase concentration in the bronchoalveolar fluid from the challenged lung became significantly higher than that in the opposite control lung (3.90 +/- 1.88 nmol/h versus 0.86 +/- 0.55). the intracellular level of beta-glucuronidase in the alveolar macrophages in the challenged lung was 40.3% lower than that in the control lung. in 3 controls i ...	1983	6134181
the late reaction following bronchial provocation with house dust mite allergen. dependence on arachidonic acid metabolism.	the involvement of arachidonic acid metabolism in early and late bronchial reactions has been studied in four asthmatic subjects sensitive to dermatophagoides pteronyssinus. pre-treatment with either indomethacin (an inhibitor of the cyclo-oxygenase pathway) or benoxaprofen (an inhibitor of both cyclo-oxygenase and lipoxygenase pathways) failed to affect the amplitude, but did produce some foreshortening of the early response to allergen. if benoxaprofen is an effective inhibitor of srs-a format ...	1983	6861377
site of airflow obstruction in immediate and late reactions to bronchial challenge with dermatophagoides pteronyssinus.	the density dependence of maximum flow in ten atopic asthmatics was assessed by response to breathing a helium-oxygen mixture. they were then subjected to bronchial challenge by dermatophagoides pteronyssinus and density dependence re-assessed during early and late bronchial reactions. there was considerable individual variation in the density dependence before challenge and in the change in density dependence during induced bronchospasm. however, for any given individual the results were simila ...	1983	6851071
the effect of inhaled verapamil on allergen-induced bronchoconstriction.	in eight patients with extrinsic bronchial asthma the effect of inhaled verapamil (3 and 6 mg) was compared with saline on bronchial challenge with extracts of dermatophagoides pteronyssinus. there was no significant difference in the baseline fev1 before and after inhalation of saline or verapamil 2.5 mg/ml. however, verapamil at a higher concentration (5 mg/ml) caused bronchoconstriction in four patients who had to be excluded from the part of the study. verapamil at both these concentrations ...	1983	6839438
reverse enzyme immunoassay for the determination of dermatophagoides pteronyssinus ige antibodies.	sera from patients with suspected mite sensitivity were assayed using both the radioallergosorbent test (rast) and a reverse enzyme immunoassay for ige antibodies (reia). of the 133 sera studied, the rast gave positive results in 48 cases and the reia in 59. negative results for both assays were obtained in 72 of the sera. the immunoenzymatic assay described here, using micro-plates as solid phase, has proven to be ige- and antigen-specific. the allergen conjugate has a prolonged shelf life and ...	1983	6343254
suppressor t cell numbers and function in atopic patients with high serum ige levels.	suppressor t cell function and the sensitivity of lymphocyte transformation to histamine has been studied in 9 atopic patients with high serum ige levels and in 14 controls. the effect of concanavalin a (con a) induced suppressor t cells on the proliferative response of fresh lymphocytes to mitogens and antigens was measured; in atopic subjects the median suppression to 5.0 micrograms/ml con a was 80% to 1.0 microgram/ml phytohaemagglutinin (pha) was 73%, to dermatophagoides pteronyssinus extrac ...	1983	6229638
[skin tests, rast and provocation tests with a purified acarian extract (pharmalgen) in respiratory hypersensitivity].	the diagnostic value of a purified and standardized extract of the house dust mite dermatophagoides pteronyssinus (pharmalgen, pharmacia) has been tested by nasal or bronchial allergic provocation tests (pt). 50 patients (31 with rhinitis and 19 with asthma) who had a skin test and rast positive for d. ptero. were tested: all had a positive pt. 47% of the patients with asthma had a late phase of bronchial obstruction. pt were negative in 18 subjects not allergic to d. ptero. it is concluded that ...	1984	6600156
[choice of positive criteria in specific bronchial provocation tests with a purified acarian extract (pharmalgen)].	bronchial provocation tests (pt) were performed with a purified and standardized extract of the house dust mite dermatophagoides pteronyssinus (d. ptero.) in an attempt to determine the most sensitive criteria of bronchospasm. nineteen asthmatic patients with skin test and rast positive for d. ptero. had a positive bronchial pt. more than 40% increase of raw insp. simultaneously with dyspnea or wheezing was found in each patient, whereas only 6/19 had a 20% increase of fev1. 47% of the patients ...	1984	6523105
hyposensitization with a tyrosine adsorbed extract of dermatophagoides pteronyssinus in adults with perennial rhinitis. a controlled clinical trial.	hyposensitization with a tyrosine adsorbed extract of dermatophagoides pteronyssinus was effective in relieving symptoms in selected patients with perennial rhinitis due to this allergen who had responded poorly to topical application of steroids. there was a significant reduction in the nasal response to allergen after six weekly injections only in the actively treated group, but symptomatic improvement greater than that produced by placebo therapy was only evident after a further 10 months of ...	1984	6388401
clinical and immunologic evaluation of tyrosine-adsorbed dermatophagoides pteronyssinus extract: a double-blind placebo-controlled trial.	we evaluated the clinical efficacy and immunologic changes associated with the administration of tyrosine-adsorbed dermatophagoides pteronyssinus (dpt) extract. the study was carried out in a double-blind, placebo-controlled fashion in 18 patients with dpt-induced asthma during a trial period of at least 12 mo. patients initially received six increasing doses once a week; the top dose of 400 noon units was repeated at monthly intervals. clinical response was on the basis of diary cards on which ...	1984	6208231
antigens and allergens from the common house dust mite dermatophagoides pteronyssinus. part i. demonstration of multiple allergens by immunochemical and biologic analyses.	the complexity of a highly allergenic water-soluble extract of the common house dust mite dermatophagoides pteronyssinus has been investigated by use of the preparative fractionation techniques of ultrafiltration, gel permeation chromatography, and isoelectric focusing, and the allergenic properties of the resultant fractions are assessed by skin prick tests, rast, and a histamine-release assay. in addition, two analytical approaches were used to identify allergens-radioimmunoelectrophoresis and ...	1984	6747134
factors influencing the occurrence of a late reaction to allergen challenge in atopic asthmatics.	thirty extrinsic asthmatics were challenged by inhalation with dermatophagoides pteronyssinus extract. in twenty-four an immediate reaction was observed and in sixteen this was followed by a late reaction. those with late reactions tended to have more severe asthma but did not report greater sensitivity to housedust mite. the occurrence of a late reaction was not related to the degree of airways obstruction before challenge or to the intensity of the immediate reaction. patients in whom the earl ...	1984	6467556
a controlled trial of hyposensitization with adsorbed tyrosine dermatophagoides pteronyssinus antigen in childhood asthma: in vivo aspects.	continuing study for a second year and further analysis of a double-blind placebo controlled trial, already briefly reported, of injections of tyrosine-adsorbed, glutaraldehyde-modified dermatophagoides pteronyssinus antigen in fifty-one children with perennial asthma and positive bronchial challenge to the antigen, confirms that the patients receiving the treatment reduced their symptomatic medication more than controls, without deterioration of symptoms. some became symptom-free, when off all ...	1984	6375890
[difference between mite antigens (dermatophagoides pteronyssinus) bound to ige of allergic patients and those bound to ige of immunized mice].		1984	6466096
t cell responses to the major allergen from the house dust mite dermatophagoides pteronyssinus, antigen p1: comparison of patients with asthma, atopic dermatitis, and perennial rhinitis.	peripheral blood mononuclear cells (pbmc) from the majority of the allergic patients that we tested who were skin test-sensitive to the house dust mite dermatophagoides pteronyssinus (d. pt.) (46/67) showed significant proliferation in response to the purified major allergen antigen p1; pbmc from 14/15 nonsensitive controls showed no significant response. optimal responses were seen with 10 micrograms antigen p1/ml, but 21 of 43 patients tested showed significant proliferation at 0.01 microgram ...	1984	6609976
antigens and allergens from the common house dust mite dermatophagoides pteronyssinus. part ii. identification of the major ige-binding antigens by crossed radioimmunoelectrophoresis.	crossed immunoelectrophoresis demonstrated 51 antigens in a water-soluble extract of the house dust mite dermatophagoides pteronyssinus. autoradiography demonstrated that 11 of the antigens bound ige antibodies in the sera of house dust mite-allergic patients. ige antibodies in 23 different sera reacted with from one to eight antigens. on the basis of lowenstein's (1978) definition of a "major allergen," two of the antigens, numbers one and 36 would be described as "major allergens." apart from ...	1984	6611362
skin test reactivity and clinical allergen sensitivity in infancy.	we examined the development of skin test reactivity and clinical allergen sensitivity in infancy. seventy-eight infants of atopic parents were skin prick tested every 4 mo from 4 to 16 mo and an additional 57 of these infants were tested at 20 mo. wheal diameters were recorded for histamine (1 mg/ml) and specific allergen reactions by use of cow's milk, egg albumen, wheat, and dermatophagoides pteronyssinus. the histamine mean wheal diameter was significantly lower at 4 and 8 mo compared to the ...	1984	6699318
[immunochemical and acarological study of mites].	the data derived in the study of dermatophagoides pteronyssinus by microscopic and immunological assays (double radial immunodiffusion in 2 modifications and indirect rat mast cell degranulation) are provided. the immunochemical assays permitting the detection of mites in 93.3% of cases have been shown to compare very favourably with the microscopic techniques that make it possible to detect mites only in 41.7% of cases.	1984	6704525
recognition of two dermatophagoides pteronyssinus-specific epitopes on antigen p1 by using monoclonal antibodies: binding to each epitope can be inhibited by serum from dust mite-allergic patients.	monoclonal antibodies were raised against antigen p1, the major allergen of the house dust mite (dermatophagoides pteronyssinus). the majority were antigen p1 specific, isotype igg1, and did not react with a comparable d. farinae allergen. these antibodies bound 38 to 50% of 125i antigen p1 in antigen-binding assays (titer greater than or equal to 1/1,000,000), and the quantities of igg antibody in ascites were 2 to 4 logs greater than those in polyclonal mouse antiserum or in serum from a mite- ...	1984	6207232
characterization of igg-subclass antibodies to individual allergens by crossed radioimmunoelectrophoresis.	a crossed radioimmunoelectrophoretic (crie) method for detection of human igg subclass specificities against individual antigens is described. after crossed immunoelectrophoresis (cie) of the antigens the immunoplates are incubated with serum, washed and incubated with mouse monoclonal anti-human igg-subclass antibodies. after washing, the plates are finally incubated with the 125i-labelled detector protein, rabbit anti-mouse igg, and washed. the plates are then placed on an x-ray film for autor ...	1984	6335630
in vitro investigations in asthmatic children undergoing hyposensitization with tyrosine-adsorbed dermatophagoides pteronyssinus antigen.	successful hyposensitization to dermatophagoides pteronyssinus in perennial childhood asthma was associated with a slight mean rise of serum igg antibody to the mite antigen in contrast to a placebo group in whom this antibody fell slightly. no relationship was detected between the effect on asthma and the magnitude of this change. nor was there any consistent effect on ige or iga antibody. there was a wide range of total serum ige and ige, igg and iga antibody to d. pteronyssinus before treatme ...	1984	6375891
a new, purified dermatophagoides farinae allergen preparation. evaluation with spt and rast techniques.	a purified, standardized and characterized allergen preparation of the house dust mite dermatophagoides farinae (df) was evaluated by skin prick test (spt) and radioallergosorbent test (rast) in 88 subjects. skin reactions to the purified df preparation and a corresponding crude df preparation were compared. spt was also performed with a crude dermatophagoides pteronyssinus (dpt) preparation to compare reactions obtained with the two mite preparations. by spt the purified df allergen preparation ...	1984	6465481
standardization of allergens. qualitative definition of house dust mite extracts following electroblotting and detection of components with antibody and lectin probes.	components of an extract of dermatophagoides pteronyssinus were electrophoretically separated, transferred to nitrocellulose membranes, and then probed with a variety of antibodies and lectins. using mite-allergic human sera as the first antibody source, ige-binding components were identified. binding patterns by monoclonal and heterologous sera were also obtained. the method is suitable for the qualitative, and ultimately the quantitative, characterization of allergen extracts. the electroblott ...	1984	6500720
[allergenic acari].	the authors report the milestones which led to the discovery of the mite dermatophagoides pteronyssinus as the main allergen source in house dust, and discuss its etiological role in allergic respiratory diseases. the present knowledge on the importance of other species of mite potentially allergenic in man is focused. from a biological point of view, the taxonomy, anatomy and physiology of these important arthropods are described. furthermore, the techniques for the collection and identificatio ...	1984	6085002
[acari of food storage facilities. an ecologic and immuno-allergic study].	the authors studied the commonest species of storage mites, living in granaries and in house dust. they found nine species of mites belonging to different systematic groups and were identified: acarus siro, tyrophagus putrescentiae, glycyphagus domesticus, lepidoglyphus destructor, chortoglyphus arcuatus, androlaelaps casalis, cheyletus fortis, cheyletus tenuipilis, thyreophagus entomophagus. skin tests were performed on 14 patients with house dust allergy, using extracts of three storage mites, ...	1985	3837918
[control of dust mites (dermatophagoides pteronyssinus) through the use of a fungicide. experimental observations. clinical trial in children allergic to dermatophagoides].		1985	3887534
[study of the purity and specificity of 2 autochthonous allergenic extracts (dermatophagoides pteronyssinus and candida albicans) using the elisa inhibition method].		1985	3912948
[cultivation of dermatophagoides pteronyssinus].		1985	3990644
[frequency of sensitization to d. pteronyssinus or d. farinae in children with perennial bronchial asthma].	bronchial sensitization against house dust mites (dermatophagoides farinae, dermatophagoides pteronyssinus) was investigated in 48 children. the therapeutic effect of hyposensitization and removal of house dust mite allergen was evaluated. the diagnosis was established by skin-prick-test, rast and bronchial provocation. the bronchial provocation test was carried out by a whole body plethysmograph with breath by breath registration of the airway resistance. we found a bronchial reaction against b ...	1985	4000137
storage mites as a cause of bronchial asthma in denmark.	from october 1982 to july 1983, 133 consecutive patients complaining of allergic symptoms were prick-tested with three storage mites (acarus siro, lepidoglyphus destructor, tyrophagus putrescentiae) and two housedust mites (dermatophagoides pteronyssinus and dermatophagoides farinae). a total of 60 patients (45%) had a positive prick-test for one or more of the storage mites. a mutual positive correlation was found between the prick-test results for the two house-dust mites as well as for the th ...	1985	4003230
allergy to guinea pigs: i. allergenic activities of extracts derived from the pelt, saliva, urine and other sources.	guinea pig-sensitive patients with asthma and rhinitis were skin test positive to extracts of several materials derived from guinea pigs. a radioallergosorbent test (rast) was developed to measure serum ige specific for the dander, urine, saliva and also for dust from the air-vent filters of a room housing guinea pigs. a strong correlation was found between positive skin test reactions, and raised serum ige to these extracts. furthermore, the relative allergenic potency of extracts was similar w ...	1985	4006174
the occurrence of indoor allergens in saudi arabia.	investigations on indoor airborne allergens in saudi arabia were performed by mold cultures and dust analyses by counter-current immunoelectrophoresis. twenty fungal genera were isolated, with aspergillus as the most often encountered. most of the dust-bound fungi found are ubiquitous and common. antibodies against dermatophagoides pteronyssinus, cat- cow- and rat dander, and cynodon dactylon pollen were used in the dust analyses. animal antigens were found in five of the ten dust samples. house ...	1985	4014781