Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| neuraminidase inhibition by chemically sulphated glycopeptides. | chemically sulphated glycopeptides (derived from pig duodenal mucosa) inhibited clostridium perfringens neuraminidase (ec 3.2.1.18) activity in a ph-dependent manner. analysis of inhibition kinetics data indicated that, although the enzyme inhibition could not be categorized into any of the classical types of inhibition, it could be interpreted as a function of the size and shape of the substrates used. the enzyme activity was inhibited by 86% and 40% when tested with bovine submaxillary-gland m ... | 1979 | 227363 |
| characterization of human lymphocytes bearing fc receptors for ige isolated from blood and lymphoid organs. | human lymphocytes isolated from adult peripheral blood and cord blood, tonsils, adenoids and spleens were analysed for fc receptors for ige (fcepsilon) by rosette assays. the fcepsilon+ cells were also characterized for their class of surface immunoglobulin (sig), complement receptors, receptors for sheep erythrocytes (e), and helix pomatia a haemagglutinin (hp), and their abilities to phagocytoze and adhere. the average number of fcepsilon+ cells was in adult peripheral blood 1.2 +/- 0.4%, in c ... | 1979 | 368962 |
| mutagenicity of plant flavonols in the salmonella/mammalian microsome test: activation of flavonol glycosides by mixed glycosidases from rat cecal bacteria and other sources. | over 70 naturally occurring and synthetic flavonoids were screened for mutagenicity with 5 tester strains in the salmonella/mammalian microsome assay: ta1535, ta100, ta1537, ta1538 and ta98. frameshift mutagenicity was confined to the flavonols (flavon-3-ols) in strain ta98, ta1537 and ta100. the two most mutagenic falvonols, namely, quercetin (3,3',4',5,7-pentahydroxyflavone) and kaempferol (3,4',5,7-tetrahydroxyflavone), exhibiting 12 and 7 revertants/nmol in ta98 respectively, are also the mo ... | 1979 | 375081 |
| [fractional protein makeup of candida utilis yeast protoplasts in the process of their growth and development]. | candida utilis ibfmy-405 was grown in a synthetic medium with glucose. cells taken at the logarithmic phase of growth were studied. the cells were treated with the enzyme from helix pomatia to prepare protoplasts which were separated by differential centrifugation into groups according to their size. three protein fractions were isolated from each group and the amino acid composition of the proteins was determined. proteins of the first fraction (cytoplasmic) prevailed in all of the protoplast g ... | 1979 | 571510 |
| a versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations. | a procedure for cell fractionation using lectin-affinity chromatography is described. it consists of a single affinity adsorbent, hog gastric mucin blood group a+h substance covalently coupled to sephadex or sepharose, to which lectins of various specificities can bind. the complex formed, lectin in equilibrium hog a+h substance-sephadex, then serves as an affinity probe for isolating and fractionating cells. the lectins from ulex europaeus, lotus tetragonolobus, helix pomatia, dolichos biflorus ... | 1979 | 573269 |
| the isolectins of helix pomatia. separation by isoelectric focusing and preliminary characterization. | helix pomatia lectin was fractionated into several (at least 12) components by isolectric focusing in pharmalytetm. eight of these were recovered from a preparative column experiment and shown to be essentially pure by analytical electrofocusing in polyacrylamide gel slabs. the lectin components had haemagglutinating activity against human group a erythrocytes and similar molecular weights of 80 000 as determined vidual components showed slight variations in composition. the components are consi ... | 1979 | 465535 |
| heterogeneity and mechanism of action of human natural killer lymphocytes: differential distribution of receptors for helix pomatia haemagglutinin (hp receptors). | neuraminidase-treated lymphocytes from the peripheral blood of normal human donors were fractionated on columns charged with helix pomatia haemagglutinin (hp) coupled to sepharose 4b. while lymphocytes lacking hp receptors (hp-) passed directly through the column (fraction i), lymphocytes with hp receptors (hp+) were subsequently eluted in two distinct fractions with two different concentrations of the competitive hapten n-acetyl-d-galactosamine (fraction ii and iii, respectively). the natural c ... | 1979 | 493880 |
| synaptic and hormonal modulation of a neuronal oscillator: a search for molecular mechanisms. | 1. the central ganglia of a number of gastropod molluscs (including the marine snail aplysia californica and the terrestrial snail helix pomatia) contain neurones which exhibit endogenous patterns of oscillatory activity. 2. this oscillatory activity can be modulated for long periods of time by synaptic and hormonal stimulation. 3. stimulation of appropriate pre-synaptic nerves causes long-lasting hyperpolarization in these neurones, with complete abolition of oscillatory activity. this synaptic ... | 1979 | 512575 |
| [preparation and properties of protoplasts from saccharomyces vini k-42]. | lytic enzymes from the edible snail--helix pomatia--were used to obtain protoplasts of yeast saccharomyces vini k-42. the yeast grown in aerobic conditions proved more sensitive. the resultant protoplasts oxidized glucose and sucrose and did not oxidize maltose. | 1979 | 515001 |
| [receptors of human red blood cells for anti-tah and anti-ahp in the course of intravascular aging]. | human o-erythrocytes aged in situ do not expose free receptors for anti-t agglutinin of arachis hypogea. new receptors for concentrated anti-a agglutinin of helix pomatia are manifested but are lost again in the course of further ageing of the cells in situ. a remasking of exposed receptors for anti-tah and anti-ahp byautologous, strongly binding globulins is supposed. these globulins could constitute the decisive signal for the autologous phagocytosis of senescent red blood cells. | 1979 | 525143 |
| [lectins as reagents for the differentiation of serum enzymes. lectins as reagents, i. (author's transl)]. | lectins from canavalia ensiformis, phaseolus vulgaris, and triticum vulgare react with arylamidase, alkaline phosphatase, gamma-glutamyltransferase, and cholinesterase of human sera by formation of enzymatically active, mostly insoluble complexes. arylamidase, alkaline phosphatase, and cholinesterase react more intensely in sera of healthy people than in sera of patients with liver and neoplastic diseases. arylesterase is bound to a distinct degree only by concanavalin a. the enzymes mentioned a ... | 1979 | 547035 |
| immuno-chemical studies on the alkali-labile carbohydrate chains of human serum glycoproteins. | human serum glycoproteins can be classified into those containing n-acetyl-d-galactosamine and into those lacking this hexosamine. the n-acetyl-d-galactosamine-containing serum glycoproteins have alkali-labile chains containing this hexosamine linked o-glycosidically to hydroxy amino acids. these alkali-labile chains can be demonstrated in neuraminic acid free serum glycoproteins by gas liquid chromatography and by using precipitating lectins from invertebrates and plants. they are represented b ... | 1979 | 762504 |
| 3,3-bis-(4-hydroxyphenyl)-7-methyl-2-indolinone (bhmi), the active metabolite of the laxative sulisatin. | the disodium salt of the sulphuric diester of 3,3-bis-(4-hydroxyphenyl)-7-methyl-2-indolinone (sodium sulisatin, laxitex), a synthetic laxative with two phenolic groups esterified with sulfate, has been studied in order to find out if its laxative properties may be attributed to the unaltered compound or to its diphenolic derivative bhmi. we first studied the effect of homogenates of the gastrointestinal tract of rats and of rat cecal content of the hydrolysis of sulfate ester bonds of sulisatin ... | 1979 | 583222 |
| helix pomatia a hemagglutinin, a surface marker for bovine t-lymphocytes. | bovine peripheral blood lymphocytes (pbl) were isolated from blood collected from 6 cattle. after treatment with neuraminidase, 40 or 60% of the cells were shown to combine with helix pomatia a hemagglutinin (hp) depending whether a direct or indirect fluorescence technique was used. about 20% of the cells were ig-bearing. with double staining fluorescence technique, it was shown that cells attaching to hp were not ig-bearing and the reverse. with the aid of hp, covalently bound to sepharose, ig ... | 1979 | 15612267 |
| a study of porcine lymphocyte populations. i. separation of porcine lymphocyte subpopulations. | several methods for separating porcine lymphocyte populations were studied. lymphocyte populations enriched in t cells were obtained by filtration on nylon fiber columns. lymphocyte populations enriched in b cells were obtained by e rosette sedimentation. results obtained with anti-immunoglobulin columns were less satisfactory. attempts to fractionate porcine lymphoid cells with the help of helix pomatia lectin remained unsuccessful. | 1980 | 15615056 |
| isolation, characterization and implications of anti-tf (thomsen-friedenreich) agglutinins from different sources. | anti-tf agglutinins from peanut (arachis hypogaea) and from vertebrate sera of different species have been successfully isolated by affinity chromatography on acid-activated sepharose 4 b. the proteins were characterized by immunoelectrophoresis, polyacrylamide gel electrophoresis in the presence of sds and with respect to their carbohydrate binding specificities. anti-tf substances from sera showed one precipitin arc in immunoelectrophoresis, but quantitative immunoprecipitation revealed our hu ... | 1980 | 6157637 |
| helix pomatia agglutinin (hpa) affinity chromatography: the isolation of pure b and t cell populations and their use for the routine hla-dr (ia) serology. | the anti-a1 agglutinin (hpa) of the albumin gland of the snail helix pomatia binds specifically to neuraminidase-treated t lymphocytes. after its immobilization on sepharose-6mb an affinity matrix is obtained which is able to separate t and b lymphocytes. in 40 independent experiments enriched t and b cell populations were isolated either by this hpa affinity technique or by e-rosettes incubated with sheep red blood cells for 2 h or 15 h. the results showed that with the hpa affinity matrix with ... | 1980 | 6766169 |
| molluscan immunity: interactions between the immunogenic bacterium pseudomonas aeruginosa and the internal defense system of the snail helix pomatia. | 1980 | 6772484 | |
| studies on the meta and para o-sulphation of the catechol compound 3,4-dihydroxybenzoic acid by rat liver sulphotransferase in vitro. | the enzymic meta and para o-sulphation of 3,4-dihydroxybenzoic acid was investigated in vitro with a dialysed high-speed supernatant from rat liver. the o-sulphated products were identified by comparison with the reference compounds. the chemical synthesis and identification of the reference o-sulphate esters is described in detail. the sulphotransferase activity of the dialysed supernatant from rat liver towards 3,4-dihydroxybenzoic acid was 580 pmol of 3-o-sulphate and 120 pmol of 4-o-sulphate ... | 1980 | 6937191 |
| protoplasts from yeast and mycelial forms of candida albicans. | protoplasts have been obtained in high yields from the yeast and mycelial forms of a variety of strains of candida albicans by enzyme digestion of cells with commercially available lytic enzymes. the protoplast formation procedure was equally effective for exponential and stationary phase cells. pretreatment with dithiothreitol and pronase in the presence of edta and tris was necessary. other thiol reagents and conditions did not release protoplasts from all the strains of c. albicans tested. tr ... | 1980 | 7014767 |
| [lipase activity of oospora lactis protoplasts]. | the paper describes how to prepare osmotically sensitive forms from the fungus oospora lactis. the lytic enzyme from actinomycetes at a concentration of 25 mg/ml causes abundant formation of protoplasts at 36 degrees c and ph 7.5 within 10--15 min. the best stabilizing agent is 1 m nacl solution or a mixture of 1 m nacl with 1 m mannitol solution (1:1). in order to induce lysis of cell walls by the enzyme from helix pomatia, the cells must be pretreated with a solution containing 40 mm tris (hyd ... | 1980 | 7190640 |
| simple test for detection of virus neuraminidase and antineuraminidase using lectins (lectin-neuraminidase test system). | the fast and sensitive detection of virus neuraminidase effectiveness (influenza virus a and b or mumps virus) and of antibodies against virus neuraminidase in human serum is described. lectin mainly obtained from arachis hypogaea and helix pomatia is used in the lectin-neuraminidase test (ln-test). the lectins are capable of agglutinating erythrocytes after virus incubation because virus neuraminidase reveals the t-antigen to be situated on erythrocytes. the presence of anti-neuraminidase in hu ... | 1980 | 7223125 |
| distribution of serotonin and dopamine receptors in aplysia tissues: analysis by [3h]lsd binding and adenylate cyclase stimulation. | the distribution of receptors for serotonin and dopamine has been studied in various neuronal and non-neuronal tissues from aplysia californica using: (1) a [3h]lsd binding assay; and (2) stimulation of adenylate cyclase activity. high levels of specific [3h]lsd binding were found in all ganglia and nerves examined. lower levels of binding were present in a number of muscle tissues and in the sheath surrounding the central ganglia. the ability of serotonin and dopamine to inhibit [3h]lsd binding ... | 1980 | 7357415 |
| human alkaline phosphatases. evidence of three isoenzymes (placental, intestinal and liver-bone-kidney-type) by lectin-binding affinity and immunological specificity. | the structural relationship between human alkaline phosphatase isoenzymes from placenta, intestine, liver, bone and kidney was investigated by lectin-binding affinity chromatography. in addition, antibody-binding sites of the enzymes were studied using monospecific antisera against each of the isoenzymes. evidence is offered for the existence of three classes of alkaline phosphatases: the placental isoenzyme, the intestinal isoenzyme and the liver-bone-kidney-type-isoenzyme: 1. a high affinity t ... | 1980 | 7437449 |
| differential expression of lectin receptors during hemopoietic differentiation: enrichment for granulocyte-macrophage progenitor cells. | molecular changes occur at the surface of hemopoietic cells during differentiation from progenitor cells to mature granulocytes and macrophages. the differential expression of surface carbohydrate residues has been probed using lectins and the results used to purify normal mouse granulocyte-macrophage progenitor cells. ten different lectins were screened for selective interaction with mouse hemopoietic colony-forming cells (cfcs), using agglutination or a quantitative analysis of the number of f ... | 1980 | 7440634 |
| increase of blood group a and loss of blood group sda activity in the mucus from human neoplastic colon. | aqueous extracts of human neoplastic and adjacent normal colonic mucosa were investigated for hemagglutination inhibition of both the anti-a serum and the dolichos biflorus lectin. the anti-a antiserum was inhibited by the extracts from neoplastic tissues to a much higher extent than by that from normal mucosa; the inhibition was strictly dependent on the blood group and the secretor status of the individual. the inhibition titers against the dolichos lectin were much lower for the tumor than fo ... | 1980 | 7466135 |
| lectin receptors on human blood and bone marrow cells and their use in cell separation. | a series of fluorescein-conjugated lectins (sophora japonica agglutinin, helix pomatia agglutinin, peanut agglutinin, the erythroagglutinin from phaseolus vulgaris, pokeweed mitogen, wheat germ agglutinin and the fucose-binding lectin from lotus tetragonolobus) were analyzed for their binding to human peripheral blood cells with a fluorescence-activated cell sorter. most of the lectins showed increasing cell binding in the order erythrocytes less than lymphocytes less than monocytes less than ne ... | 1980 | 7470630 |
| lectin receptors as markers for trypanosoma cruzi. developmental stages and a study of the interaction of wheat germ agglutinin with sialic acid residues on epimastigote cells. | trypanosoma cruzi at various stages of maturation and differentiation have been isolated by conventional cellular fractionation procedures and characterized by cell surface markers using 30 highly purified lectins encompassing all known sugar specificities. cell surface carbohydrates of the various t. cruzi stages were analyzed by agglutination and lectin-binding assays. specific receptors for wheat germ agglutinin (wga), helix pomatia, sophora japonica, and bandeiraea simplicifolia lectin ii we ... | 1980 | 7000967 |
| characterization and response to mitogens of mammary lymphocytes from the bovine dry-period secretion. | from bovine mammary secretion during the dry period, the total number of cells was between 1.2 and 5.9 x 10(6)/ml. a mean of 35% of these cells were classified as lymphocytes and approximately 85% of them could be isolated by the ficoll-isopac method. centrifugation separated 6% of the cells into the fat; 5% of them were lymphocytes. about 47% of the lymphocytes bound helix pomatia agglutinin, a t-cells marker, while the proportion of ig-bearing cells was approximately 28%. the mammary lymphocyt ... | 1980 | 7005279 |
| induction of tryptophan oxygenase and tyrosine aminotransferase by metabolites of hydrocortisone. | metabolites of hydrocortisone were isolated from rat liver on a preparative scale, fractionated by column chromatography on sephadex lh-20 and silica gel and tested for biological activity. apart from the well known neutral metabolites, steroid glucuronides and sulfates, we obtained metabolite fractions containing non-conjugated steroidal carboxy acids and acid metabolites of unknown structure. one of these fractions induced tyrosine aminotransferase (ec 2.6.1.5) in adrenalectomized female rats ... | 1980 | 6111341 |
| binding of lectins to leukemic cell lines. | the binding of 12 different fluorescein-conjugated lectins to 10 all (acute lymphoblastic leukemia) cell lines and two cell lines derived from patients in blast crisis of myeloid leukemia was examined. the specificity of the membrane fluorescence was demonstrated by inhibition with various saccharides. three of the lectins bound to all cell lines, four bound to only some of the lines, and five were not bound. there was no correlation between the binding pattern and the immunological phenotype of ... | 1980 | 6928109 |
| n-hydroxyphenacetin, a new urinary metabolite of phenacetin in the rat. | n-hydroxyphenacetin has been found in the urine of rats dosed with phenacetin, extending previous reports that phenacetin is n-hydroxylated by liver microsomes in vitro. after an oral dose of phenacetin (500 mg/kg) urine was collected for 24 hr, conjugates hydrolyzed with extract of helix pomatia, and the metabolites extracted with dichloromethane and treated with diazomethane. methylation of n-hydroxyphenacetin produced a stable derivative, n-methoxyphenacetin, which was separated from most oth ... | 1981 | 6113936 |
| unusual lectin-binding properties of a herpes simplex virus type 1-specific glycoprotein. | lysates from herpes simplex virus type 1-infected cells were subjected to affinity chromatography on soybean and helix pomatia lectins. one of the virus-specified glycoproteins, probably the herpes simplex virus type 1-specific gc glycoprotein, bound to the lectins and was eluted with n-acetylgalactosamine. the affinity chromatography permitted a high degree of purification of the type-specific glycoprotein with respect to both host cell components and other viral glycoproteins. the lectin affin ... | 1981 | 6264128 |
| [isolation of yeast protoplasts using various preparations of the hepato-pancreatic juice of helix pomatia]. | conversion of large amounts of saccharomyces cerevisiae cells to protoplasts is studied using various preparations extracted from helix pomatia hepato-pancreatic juice. the most favourable yield in two hours incubations (88 per cent) is obtained with 20 ml cytohelicase, a chitinase and glucanase enriched extract, per 400 g of yeast cells, harvested at the end of the logarithmic growth phase and preincubated in presence of 2-mercaptoethanol. | 1981 | 7011422 |
| lectin-binding patterns of small lymphocytes in bone marrow, thymus and spleen: demonstration of lymphocyte subsets by quantitative radioautography. | cells from mouse bone marrow, thymus and spleen were exposed to 125i-labeled concanavalin a (con a). lens culinaris lectin (lcl), soybean agglutinin (sba), helix pomatia agglutinin (hpa), phytohemagglutinin-p (pha-p), peanut agglutinin (pna), or wheat germ agglutinin (wga) in a range of concentrations and examined radioautographically. small lymphocytes in the three organs differed in the minimal concentration of each lectin which gave detectable surface labeling, while at optimal lectin concent ... | 1981 | 6895730 |
| the occurrence of thomsen-friedenreich receptors in bovine erythrocytes of various fv genotypes. | in the v/v type of bovine erythrocytes (be), the antigen im and the thomsen-friedenreich (tf) receptors, which reacted with a tf-specific agglutinin from human sera and the lectin from helix pomatia, were all destroyed by treatment with trypsin. unlike the v/v, in the f/f type of be the antigen im and these tf receptors were not only not destroyed by trypsin, but this enzyme treatment was, in fact, necessary to make them amenable to the corresponding agglutinins. another tf receptor, which react ... | 1981 | 7024419 |
| cell surface characteristics of human histiocytic lymphoma cell lines. ii. expression of helix pomatia a hemagglutinin binding surface glycoproteins, hla-dr and common acute lymphocytic leukemia (call) antigen. | 1981 | 6943398 | |
| the distribution of helix pomatia lectin receptors on mouse lymphoid cells and other tissues. | the distribution of receptors for the helix pomatia lectin on mouse lymphoid cells and other tissues was investigated. using a sensitive rosetting assay combined with immunofluorescence, lectin receptors were found on the membrane of approximately 90% of peripheral t lymphocytes, 75% of thymocytes, 30% of bone marrow cells, 20% of nude spleen cells, 15-50% of peritoneal exudate macrophages, and a subpopulation of peritoneal exudate mast cells. the thy-1-positive nude spleen cells were predominan ... | 1981 | 6973473 |
| b lymphocyte specificity of lectins of cepaea nemoralis and dolichos biflorus: paradoxical binding of anti-a active lectins to human lymphocyte subclasses. | binding of four blood group a-specific lectins, from helix pomatia (hp), lucorum (hl), cepaea nemoralis (cn) and dolichos biflorus (db) to human lymphocytes was determined. use was made of lectins attached to latex particles, as convenient probes to detect binding of lectins to individual cells, specificity of lectin-latex probes was demonstrated by comparison with agglutination and fluorescent lectin binding; experiments with untreated and enzyme-treated human and animal erythrocytes confirme ... | 1981 | 6975674 |
| [immunogenic activity of hemocyanin from the hemolymph of helix pomatia and octopus vulgaris]. | the immunogenic activity of helix pomatia haemocyanin (hph) and octopus vulgaris haemocyanin (ovh) has been tested in rabbits. primary and secondary antibody response has been evaluated in sera by agar double immuno-diffusion test (ouchterlony), counter immunoelectrophoresis and complement fixation. bath haemocyanins proved highly antigenic: antibody titers rising up to 1/3.200 after the second antigenic challenge. the ouchterlony test showed a partial identity between h.p.h. and o.v.h. no adver ... | 1981 | 6794581 |
| morphine glucuronide hydrolysis: superiority of beta-glucuronidase from patella vulgata. | beta-glucuronidase from patella vulgata, helix aspersa, helix pomatia, and bovine liver were evaluated for usefulness in routine hydrolysis of drug-glucuronic acid conjugates from equine urine samples. factors affecting the reaction rate (enzyme concentration, ligand concentration, temperature, and ph) were optimized. a 3-h incubation at 65 degrees c with 5000 u of beta-glucuronidase from p. vulgata per milliliter of urine resulted in complete hydrolysis of all morphine glucuronide in the urine ... | 1982 | 6799227 |
| lectins as markers of human epidermal cell differentiation. | the expression of sugar residues on human epidermal cells was investigated by means of lectin binding, as a way of determining membrane structural changes occurring during the differentiation of the epidermis. fourteen lectins of different sugar specificity were conjugated with fluorescein isothiocyanate (fitc-lectins) and tested in fluorescence microscopy on frozen sections of normal human epidermis. in parallel, fitc-lectins were tested on psoriatic-involved epidermis to visualize differences ... | 1982 | 6816653 |
| helix pomatia receptors on rat t lymphocytes and bone marrow cells. | helix pomatia (hp) receptors are present on approximately 60 percent of neuraminidase (nanaase)-treated rat t lymphocytes. the hp+ spleen cells (spc) can be separated from hp-, immunoglobulin-bearing (ig+) cells by affinity chromatography. the hp+ spc mediate the local graft-versus-host reaction (gvhr). less than 1% of rat lymphocytes bear both hp and ig markers. approximately 25% of lewis rat bone marrow cells are hp+ (only 1% are hp+ ig+), suggesting that the hp receptor may be a differentiati ... | 1982 | 6983492 |
| determination of salivary abh-blood group antigens by rocket affinoelectrophoresis. | a rocket affinoelectrophoretic assay system was used to detect and quantify the human salivary abh-blood group antigens of 155 individuals. in this system blood group antigens precipitated as rockets in agarose gels containing different lectins, with the rocket height being correlated to the antigen concentration. this technique was compared with the classical hemagglutination inhibition method for determination of salivary blood group antigens, and it was demonstrated that salivary a, b and h b ... | 1982 | 7072191 |
| use of lectins for detection of glycoconjugates in the glandular cells of the human bronchial mucosa. | paraffin-embedded human bronchial biopsies, obtained in macroscopically healthy areas, were examined using nine peroxidase-bound lectins. these were either isolated or purified by affinity column chromatography (ulex europeus, triticum vulgare, glycine max, and arachis hypogatea lectins) or commercial preparations (lotus tetragonolobus, canavalia ensiformis, helix pomatia, phaseolus vulgaris, and lens culinaris lectins) and conjugated to peroxidase (except for concanavalin a). these labeled lect ... | 1982 | 7130674 |
| detection and quantitation of 19-norandrosterone in urine by isotope dilution-mass spectrometry. | a highly accurate method has been developed for detection and quantitation of 19-norandrosterone, the major urinary metabolite of 19-nortestosterone in man. a suitable 14c labelled standard was obtained by i.m. injection of [4-14c]-19-nortestosterone into a human volunteer. a fixed amount of this internal standard was added to a fixed amount of urine and the mixture was treated with helix pomatia for 24 h. after extraction and purification by t.l.c., the mixture was converted into methoxime-trim ... | 1982 | 7132356 |
| induction of macrophage-mediated tumor lysis by an animal lectin, sarcophaga peregrina agglutinin. | several animal lectins, such as sarcophaga peregrina agglutinin, limulus polyhemus agglutinin, helix pomatia agglutinin and helix aspersa agglutinin, were tested for induction of tumor lysis mediated by macrophages. among them, only s. peregrina agglutinin purified from the hemolymph of s. peregrina larvae lysed tumor cells in co-operation with macrophages from the peritoneal cavity of mice. s. peregrina agglutinin alone did not kill target tumor cells. macrophages in the presence of this lectin ... | 1982 | 7152198 |
| fructose-bisphosphate aldolase from helix pomatia. | 1982 | 7154953 | |
| molluscan immunobiology: isolation of an aeromonas formicans which escapes the internal defense system of helix pomatia. | bacterial infections are surprisingly rare in wild populations of gastropod molluscs. a strain of aeromonas formicans, which has now been isolated, kills helix pomatia, a land snail, when injected at doses greater than 10(6) viable cells g-1 snail weight. koch's postulates were fulfilled with this organism. initial clearance of aeromonas from the circulation was followed within hours by a bacteremia; since neither culture supernatants, killed cells nor cell extracts killed helix, there is eviden ... | 1982 | 7160512 |
| surface modifications in the platelets of a patient with alpha-n-acetyl-d-galactosamine residues, the tn-syndrome. | the tn-syndrome is an acquired disorder characterized by the polyagglutination of blood cells and the pathological exposure of alpha-n-acetyl-d-galactosamine residues (tn-antigen) at the cell surface. we now report studies on the platelet of a patient (ba.) of which 81% reacted positively with a fluorescein conjugate of helix pomatia agglutinin (hpa). the surface proteins of ba. platelets were labeled with 125i by the lactoperoxidase-catalyzed procedure; single and two-dimensional electrophoresi ... | 1982 | 7174794 |
| differences of glycoconjugates exposed on hypernephroma and normal kidney cells. | we analyzed the outer cell membranes of normal kidney cells, hypernephroma cells, nude mouse transplants of hypernephroma tissue, and cell lines derived from hypernephroma using horseradish peroxidase-coupled lectins of different specificity, in order to investigate their binding to cryostat sections or the cell surfaces of suspended single cells. we showed that hypernephroma cells express higher amounts of receptors for robinia pseudoacacia lectin, soy bean lectin and phaseolus vulgaris lectin ... | 1982 | 7176041 |
| clonal expression of the tn antigen in erythroid and granulocyte colonies and its application to determination of the clonality of the human megakaryocyte colony assay. | to evaluate whether exposure of tn determinants at the surface of human erythrocytes, platelets, and granulocytes could arise from a somatic mutation in a hemopoietic stem cell, burst-forming unit erythroid (bfu-e) colonies, colony-forming unit granulocyte-macrophage (cfu-gm), and colony-forming unit-eosinophil (cfu-eo) were grown from a blood group o patient with a typical tn syndrome displaying two distinct populations (tn(+) and tn(-)) of platelets, granulocytes, and erythrocytes. a large num ... | 1982 | 6175663 |
| natural and antibody-dependent killer cells in the thymus. | thymocytes contain a small population of light (o = 1.067), large cells with high cytolytic potential against natural killer target as well as against igg- or igm-coated erythrocytes and nucleated cells, thus displaying natural killer and antibody-dependent cellular cytotoxicity functions, but not exhibiting t killer cell capacity. as far as characterized, the cells show t cell characteristics: they are nonadherent, nonphagocytic, surface immunoglobulin-negative, c3 receptor-negative, partly fc ... | 1982 | 6185347 |
| localization of t-lymphocyte areas in human lymphoid tissues by fluorochrome-labelled helix pomatia a haemagglutinin. | fluorochrome-labelled helix pomatia a haemagglutinin (hp) stained t-dependent areas in cryostat tissue sections of human lymph node, tonsil, and spleen and thymocytes within thymic medulla but not within thymic cortex. neuraminidase treatment of the tissue section was a prerequisite for the positive cell staining. the staining was cell-membrane-associated, and the histological pattern was essentially the same as that observed in parallel sections treated with monoclonal antibodies to t cells (ok ... | 1982 | 6753127 |
| different expression of lyt differentiation antigens and cell surface glycoproteins by a murine t lymphoma line and its highly metastatic variant. | cloned lines of the methylcholanthrene-induced dba/2 t lymphoma eb and its highly metastatic variant line esb were analyzed for differences in the expression of serologically detectable cell surface differentiation markers. flow cytofluorographic analysis of cells stained with fluorescein isothiocyanate-conjugated monoclonal rate anti-mouse thy-1, lyt-1, lyt-2 and complement-dependent cytotoxicity with mouse alloantisera against lyt-3.2 and ly-6.2 revealed, for the parental low metastasizing lin ... | 1982 | 6124426 |
| identification of major sulfate conjugates in the metabolism of propranolol in dog and man. | a large portion of the dose of propranolol in animals and man is unaccounted for. using radiotracer and hplc techniques, five previously unrecognized polar and labile metabolites were found in dog urine, together accounting for 34% of the urinary radioactivity. the two main metabolites, peak 2 (7% of the radioactivity) and peak 4 (17%) could be isolated and purified by butanol extraction and reversed phase hplc. direct probe ms analysis of the main peak 4 and gc/ms analysis of the same peak afte ... | 1983 | 6137341 |
| [a simple method for radioimmunoassay of total estrogen in urine]. | a simple and rapid radioimmunoassay (ria) without extraction or purification was developed for total estrogen (conjugated and unconjugated estrogen) in urine from normal men and nonpregnant women. antiserum used to ria was produced by immunizing rabbits with estriol-16 alpha-glucuronide (e3-16-g)-bsa conjugates. antiserum to e3-16-g.bsa significantly cross-reacted with e1 (100%), e2 (100%), e3 (100%), e3-16-g (100%), and e3-17-g (100%) and did not react with other conjugated estrogen in urine. u ... | 1983 | 6299812 |
| regional distribution of n-acetyl-d-galactosamine residues in the glycocalyx of glomerular podocytes. | helix pomatia lectin (hpl) bound to colloidal gold was used as a specific cytochemical probe for the localization of terminal nonreducing n-acetyl-d-galactosamine residues in thin sections of rat kidney. in the glomerulus, lectin-binding sites were associated only with the podocyte foot process bases and were not found on the free cell surface of podocytes or on any other glomerular components. gold-particle label was often arranged in the form of clusters which extended from the foot process ba ... | 1983 | 6302101 |
| populations of herpes simplex virus glycoprotein gc with and without affinity for the n-acetyl-galactosamine specific lectin of helix pomatia. | two fractions of herpes simplex virus glycoprotein gc were isolated and characterized by means of immunosorbent-purification with monoclonal antibodies against gc and helix pomatia lectin (hpa) affinity chromatography. about 25 per cent of the glycoprotein gc population demonstrated affinity for the lectin, compatible with presence of n-acetylgalactosamine as terminal sugar of the oligosaccharide. the hpa-binding populations of gc appeared as two electrophoretic bands with lower molecular weight ... | 1983 | 6305311 |
| solubilization and partial purification of putative calcium channels labelled with [3h]-nimodipine. | high-affinity binding sites for the potent 1,4-dihydropyridine calcium channel blocker [3h]-nimodipine were solubilized from guinea-pig skeletal muscle microsomes with digitonin and chaps [3-(3-cholamidopropyl)-dimethyl-ammonio-l-propanesulfonate]. detergent-solubilized binding sites could not be sedimented by centrifugation (50,000 x g, 4 h), passed freely through 0.2 micron nitrocellulose filters and were stable at 4 degrees c with half-lives of greater than 60 h. the solubilized 1,4-dihydropy ... | 1983 | 6314149 |
| glycoprotein c of herpes simplex virus type 1: characterization of o-linked oligosaccharides. | in contrast to other viral glycoproteins, the herpes simplex virus (hsv) glycoprotein c(gc) binds to the n-acetylgalactosamine-specific helix pomatia lectin (hpa). in the present paper gc was purified by affinity chromatography with monospecific antibodies and the purified glycoprotein was subjected to protease digestion. hpa-binding protease-resistant glycopeptides were isolated by lectin affinity chromatography. the isolated structures did not bind to concanavalin a and seemed to lack charged ... | 1983 | 6319556 |
| identical lectin binding patterns of human melanocytes and melanoma cells in vitro. | cell surface glycoconjugate patterns of human epidermal cells and of melanoma cells (mc) in primary culture derived from 11 primary and metastatic melanomas were investigated using fluorescent and horseradish peroxidase conjugated lectins for visualization at the light and electron microscopic level. the lectin labeling profiles of human melanocytes (m) and mc were found to be identical. according to their binding patterns, the lectins tested were grouped into three categories: (1) lectins bindi ... | 1983 | 6187869 |
| application of lectin--gold complexes for electron microscopic localization of glycoconjugates on thin sections. | a method is described for the electron microscopic detection of lectin-binding sites in different cellular compartments and extracellular structures that uses thin sections from resin-embedded tissues. various lectins (ricinus communis lectin i and ii, peanut lectin, lotus tetragonolobus lectin, ulex europeus lectin i, lens culinaris lectin, helix pomatia lectin, and soybean lectin) were bound to particles of colloidal gold and used for direct staining of thin sections or glycoprotein--gold comp ... | 1983 | 6190857 |
| phenotypic and cytogenetic characteristics of a new epstein-barr virus negative cell line (skw 4) derived from a b-cell lymphoma. | a new epstein-barr virus nuclear antigen (ebna) negative cell line skw 4 has been established in vitro from a patient with diffuse histiocytic lymphoma. the skw 4 seems to be an authentic human tumour cell line as evidenced by its ebv negativity, monoclonality and aneuploidy tested during early in vitro passage. the cell line expresses surface mu and kappa-chains, hla-dr antigen, c3 and fc receptors and b-cell lineage antigens. the karyotypic analyses demonstrated many numerical and structural a ... | 1983 | 6329938 |
| detection and quantitation of 3 alpha-hydroxy-1-methylen-5 alpha-androstan-17-one, the major urinary metabolite of methenolone acetate (primobolan) by isotope dilution--mass spectrometry. | a highly accurate method has been developed for detection and quantitation of 3 alpha-hydroxy-1-methylen-5 alpha-androstan-17-one, the major urinary metabolite of methenolone acetate (primobolan) in man. unlabelled as well as 2h-labelled 3 alpha-hydroxy-1-methylen-5 alpha-androstan-17-one were synthesized from 1-methylen-5 alpha-androstane-3,17-dione. a fixed amount of the internal standard was added to a fixed amount of urine and the mixture was treated with helix pomatia for 24 h. after extrac ... | 1983 | 6339822 |
| evaluation of labelling methods for bovine t and b lymphocytes. | two lectins, one from helix pomatia (hp) and one from peanut (pn), were evaluated as bovine t cell markers. hp attaches to about 40% of the bovine peripheral blood lymphocytes (pbl). with an indirect technique about 60% of pbl are hp positive, while pn attaches to a slightly higher proportion of the pbl. in double labelling experiments, hp was shown to bind only to ig negative cells while 2% of the pbl were double labelled with pn and anti-ig. the influence of different pretreatments of the pbl ... | 1983 | 6346664 |
| relationship between mouse lymphocyte receptors for peanut agglutinin (pna) and helix pomatia agglutinin (hpa). | the relationship between mouse lymphocyte receptors for peanut agglutinin (pna) and helix pomatia agglutinin (hpa) has been investigated by immunofluorescence (cocapping) and radiolabeling. in neuraminidase-treated and untreated thymocytes there are two groups of glycoproteins which bind roughly equivalent amounts of pna. one group also carries all the detectable receptors for hpa, the other binds only pna. binding inhibition experiments suggest that pna and hpa receptors are in close proximity ... | 1983 | 6357809 |
| c3 receptors on human lymphocyte subsets and recruitment of adcc effector cells by c3 fragments. | the presence of c3 receptors on human peripheral blood lymphocytes (pbl) and on the adcc-exhibiting subset (k cells) thereof was analyzed by rosetting with bovine erythrocytes (eb) or chicken erythrocytes (ec) carrying human c3b, c3bi, or c3d. the indicator cells were coated with 20,000 to 100,000 c3 fragments, obtained by c3 activation with purified proteins of the alternative pathway and trypsin treatment. adcc was studied at the cellular level by means of a plaque assay, with complement-free ... | 1983 | 6222118 |
| different t cell antigens and receptors for peanut agglutinin and helix pomatia agglutinin on steroid-sensitive and resistant lymphocytes in the rabbit using double immunofluorescence. | rabbit t lymphocytes were characterized using fluorochrome-labelled antisera against thymus cell determinants and fluorochrome-labelled lectins. three anti-t cell antisera were used, detecting different antigenic determinants on the majority of t cells. only small subpopulations were found stained by one of the three antisera. after dexamethasone (dx) treatment, the proportion of t cells was significantly increased in bone marrow and appendix, presumably by different mechanisms. cells binding pe ... | 1983 | 6409802 |
| in situ-differentiation of lymphocytes in fixed tissues with peroxidase-labeled helix pomatia lectin: revelation of the helix pomatia lectin receptor on cell surfaces. application in lymph node tissues and cutaneous lymphomas. | a receptor for helix pomatia lectin (hpl) occurs on 71.5% of peripheral blood lymphocytes. with double incubation techniques we could show that the population of hpl-positive lymphocytes is by far (greater than 90%) identical with the population of t lymphocytes, as determined by "classical" markers (e rosetting) and newly developed t cell markers like okt3 or anti-human lyt3 monoclonal antibodies. in formaldehyde-fixed lymph node sections we could demonstrate the homing area of hpl-positive lym ... | 1983 | 6412296 |
| [ultrastructural detection of the heterogeneity of glycocalyx in convoluted and straight proximal tubules of rat kidney by the lectin-gold complex technic]. | a new histochemical technique in which lectin-gold complexes are applied directly to thin and semi-thin sections of tissue was used to determine the distribution of helix pomatia lectin binding sites in the proximal tubule of rat kidney. whereas a strong labeling was seen on the brush border and baso-lateral plasma membranes of convoluted tubules, the plasma membrane of cells from the medullary straight segment was unlabeled. some profiles at the frontier between cortex and medulla had a mosaic ... | 1983 | 6419990 |
| neuraminidase in bacteroides fragilis. | a neuraminidase from bacteroides fragilis was purified 542-fold by isoelectric focusing, adsorption chromatography on affi-gel 202, and gel filtration chromatography on sephadex g-200. on isoelectric focusing the neuraminidase was resolved into three differently charged fractions with pi values of 6.8, 7.1, and 7.4. the major component of pi 7.1 was used for further purification. the purified enzyme had optimal activity at ph 6.4 with n-acetylneuraminlactose as the substrate. its molecular weigh ... | 1983 | 6614909 |
| glycolipid-lectin interactions: detection by direct binding of 125i-lectins to thin layer chromatograms. | glycolipids that bind 125i-labeled lectins are detected by autoradiography after thin layer chromatography of glycolipid standards or crude lipid extracts. soybean agglutinin, bandeiraea simplicifolia i isolectins a4 and b4, and helix pomatia lectin are used to detect corresponding cell surface, glycolipid receptors in human and bovine erythrocytes. when lipid extracts from a and ab erythrocyte stroma are analyzed with helix pomatia lectin, a polymorphic expression of blood group a glycolipid de ... | 1983 | 6615536 |
| peripheral nervous system myelin and schwann cell glycoproteins: identification by lectin binding and partial purification of a peripheral nervous system myelin-specific 170,000 molecular weight glycoprotein. | radioiodinated lectins were used to detect glycoproteins of peripheral nervous system (pns) myelin (rat, human, bovine) and cultured rat schwann cells. proteins were resolved by sodium dodecyl sulfate-polyacrylamide slab gel electrophoresis and transferred to nitrocellulose filters. the filters were overlaid with radioiodinated lectins of known saccharide affinities. these included concanavalin a, helix pomatia, limulus polyphemus, maclura pomifera, peanut, soybean, ulex europaeus, and wheat ger ... | 1983 | 6619865 |
| polymorphonuclear leukocyte-mediated cytolysis induced by animal lectin. | nine animal lectins, i.e., sarcophaga peregrina agglutinin, balanus roseus agglutinin, aplysia kurodai agglutinin, balanus balanoides agglutinin, tetraclita squamosa japonica agglutinin, misgurnus anguillicaudatus lectin, asterina pectinifera agglutinin, helix aspersa agglutinin and helix pomatia agglutinin, were tested for induction of cytolysis mediated by polymorphonuclear leukocytes. among them, s. peregrina agglutinin and b. roseus agglutinin lysed murine target cells in co-operation with p ... | 1983 | 6628907 |
| subsets of epidermal langerhans cells as defined by lectin binding profiles. | in this study we characterize the cell surface glycoconjugate moieties of strain 2 guinea pig epidermal langerhans cells (lc) in single cell suspension by using a battery of 17 fluorescent lectins. all lc displayed binding sites for concanavalin a, succinylated concanavalin a, lens culinaris agglutinin, pisum sativum agglutinin, wheat germ agglutinin, succinylated wheat germ agglutinin, griffonia simplicifolia agglutinin i, ricinus communis agglutinin i, phaseolus vulgaris e agglutinin, and phas ... | 1983 | 6631049 |
| the fate of dibenz[b,f]-1,4-oxazepine (cr) in the rat. part ii. metabolism in vitro. | cr (dibenz[b,f]-1,4-oxazepine) is metabolized by rat liver 105 000 g supernatant fractions by (a) ring opening and reduction to 2-amino-2'-hydroxymethyldiphenyl ether and (b) oxidation at c11 to give a cyclic lactam. reaction (a) is nadph-dependent, decreased by dialysis and methylene blue, whereas reaction (b) is heat-resistant, inactivated by dialysis, inhibited by cn-, p-chloromercuribenzoate, amytal and menadione, and stimulated by methylene blue, phenazine methosulphate and 2,6-dichlorophen ... | 1983 | 6636832 |
| procedure for the gas chromatographic-mass spectrometric confirmation of some exogenous growth-promoting compounds in the urine of cattle. | as gas chromatography-mass spectrometry is the most conclusive confirmation technique available today for the detection of ppb levels of anabolics in the urine of cattle, the following procedure was used. the urine is hydrolysed with helix pomatia intestinal juice, extracted, and the extract cleaned by gel-permeation chromatography or with extrelut. in one fraction are eluted diethylstilboestrol, dienoestrol, hexoestrol, methyltestosterone, ethinyloestradiol, zeranol and trenbolone. this fractio ... | 1983 | 6672034 |
| simple enzymatic detection method for urinary sulfated 7 alpha-hydroxy bile acids in normal subjects and in patients with acute hepatitis. | urinary sulfated primary bile acids, 7 alpha-hydroxy bile acids, are detected by an enzymatic method using 7 alpha-hydroxysteroid dehydrogenase (ec 1.1.1.-, 7 alpha-hsd) after chromatographic fractionation on sephadex g-25. urinary sulfated or glucuronated bile acids are hydrolyzed by beta-glucuronidase/sulfatase (ec 3.2.1.31/ec 3.1.6.1) from helix pomatia and then released 7 alpha-hydroxy bile acids are detected with 7 alpha-hsd in the presence of beta-nd+, diaphorase (ec 1.6.99.2, from clostri ... | 1983 | 6574134 |
| expression of helix pomatia (hp) haemagglutinin receptors on cytolytic lymphocytes activated in mixed cultures. | the expression of receptors for helix pomatia (hp) haemagglutinin, a t cell marker, was assayed on human lymphocytes cultivated with k562 or allogeneic lymphocytes. the receptor was detected on the cells after neuraminidase treatment by reactivity with fitc conjugated hp. by affinity chromatography the lymphocyte populations were separated into 3 subsets: (1) a subset which did not attach to the lectin column; (2) and (3) two subsets attaching with different avidities, and therefore eluting with ... | 1983 | 6600771 |
| characterization of effector cells mediating igg and igm antibody-dependent cellular cytotoxicity. | spleen effector cells for igg- and igm-induced antibody-dependent cellular cytotoxicity (adcc) were characterized with respect to density and cell surface markers by using sheep erythrocytes (srbc) coated with hybridoma-derived monoclonal anti-srbc igg or anti-srbc igm antibodies as targets. while basically the same effector cells are cytolytic for igg and igm antibody-coated srbc, they differ with respect to their relative killing capacity for igg- versus igm-coated target cells. on the basis o ... | 1983 | 6601820 |
| fractionation of mouse cytotoxic t cells by use of lectins. | mouse cytotoxic t lymphocytes (ctls), induced in vivo and in vitro in mixed-lymphocyte cultures, were fractionated into agglutinated and unagglutinated cells by use of various lectins. cytolytic activity was enriched in the cell fraction agglutinated by various 2-acetamido-2-deoxy-alpha-d-galactopyranose-specific lectins, namely, dolichos biflorus agglutinin (dba), helix pomatia agglutinin (hpa), and phaseolus limensis agglutinin (lba). only a little cytolytic activity remained in the unagglutin ... | 1983 | 6605191 |
| evidence for carbohydrate-deficient forms of the major sialoglycoproteins of human platelets, granulocytes and t lymphocytes in individuals with tn syndrome. | 125i-helix pomatia and 125i-wheat germ lectins were used as probes to visualise membrane glycoproteins of circulating blood cells from individuals with tn syndrome. cells were solubilized in triton x-100 and subjected to sodium dodecyl sulphate polyacrylamide gel electrophoresis prior to incubation with iodinated lectins. normal platelets, granulocytes and t lymphocytes have a single major sialoglycoprotein of mol. wt. 143,000, 115,000 and 115,000 respectively. in tn platelets, granulocytes and ... | 1983 | 6605304 |
| lectins as probes for identification of tumor-associated antigens on urothelial and colonic carcinoma cell lines. | in the search for tumor-associated antigens, seven lectins were used to investigate the cellular distribution of membrane-associated glycoproteins on a panel of human cells derived from tumor or normal tissues. surface labelled lysates of the different cells were precipitated with the lectins and the precipitates were separated on sds-page. comparison of the autoradiographic patterns revealed that a la-reactive 115k glycopeptide (gp 115) was present on transitional-cell carcinoma cells of the ur ... | 1983 | 6826254 |
| lectin-defined cell surface glycoconjugates of pancreatic cancer cells and their nonmalignant counterparts. | alterations of cell surface carbohydrates of human pancreatic cancer cells from long-term cultures (colo 357, rpmi 7451, pc 103, pc 107) were assessed ultrastructurally by use of an array of lectin-enzyme conjugates, and compared with lectin-defined changes of glycoconjugates on human pancreatic tissue sections of normal and various pathological conditions. ulex europeus and, to a lesser degree, lotus tetragonolobus lectin binding indicate that l-fucose-containing glycoconjugates are expressed p ... | 1983 | 6832449 |
| interaction of the brush-border hydrolases of the human small intestine with lectins. | the role of human intestinal brush-border membrane hydrolases in lectin binding to the apical region of the enterocyte was investigated. the crossed-immunoelectrophoresis method was used to identify six hydrolases before and after interaction with nine lectins. three lectins (phaseolus vulgaris, ulex europeus, and ricinus communis 120 agglutinins) bound the hydrolases of 5 different subjects. helix pomatia agglutinin interacted with only 1 subject. | 1983 | 6884583 |
| lectin-binding components of normal granulocytes and leukaemic myeloid cells. | a panel of lectins was used to analyse glycoproteins of normal granulocytes and leukaemic myeloid cells. the glycoproteins of detergent-solubilized whole cells were separated by sodium dodecyl sulphate/polyacrylamide-gel electrophoresis and their lectin-binding properties determined by incubation of the fixed gels with radioiodinated lectins. normal granulocytes and leukaemic myeloid cells in different stages of maturation possess a cell-surface sialic acid-rich glycoprotein of apparent mol.wt. ... | 1983 | 6577859 |
| ultrastructure of synaptic connections of a bimodal pacemaker giant neuron in the central nervous system of helix pomatia l. | following intracellular labelling with horseradish peroxidase, the arborization and synaptic connections of the bimodal pacemaker giant neuron (rpal) of helix pomatia were investigated in the right parietal and visceral ganglia. the rpal neuron possesses extensive axonal branching, the elements of which could be observed and traced within the entire neuropil region of both ganglia. the main axonal branches showed further arborization. the thin axon processes enter the synaptic neuropil, where th ... | 1983 | 6856088 |
| synaptic organization of a multifunctional interneuron in the central nervous system of helix pomatia l. | the morphology, axonal arborization and ultrastructure of synaptic connections of the v21 giant neuron in the visceral ganglion of the snail helix pomatia has been investigated following intracellular labelling with horseradish peroxidase. the v21 neuron establishes several afferent and efferent axo-axonic connections, mainly along the first half of the primary axon. collaterals of 200-300 micron length originate from the primary axon, which shows further arborization, and both afferent and effe ... | 1984 | 6467342 |
| myocardial efficiency in the isolated ventricle of the snail, helix pomatia l. | 1984 | 241539 | |
| flow cytometric analysis of the binding of eleven lectins to human t- and b-cells and to human t- and b-cell lines. | the relative surface binding of 11 lectins to human peripheral blood t- and b-lymphocytes, to molt-4 and jm t-cell lines, and to 6410 and nc37 b-cell lines was determined by flow cytometry. the lectins from lens culinaris (lca), ricinus communis (rca), arachis hypogaea (pna), abrus precatorius (apa), ulex europaeus (uea-f), sarothamnus scoparius (sas-f), helix pomatia (hpa), phaseolus coccineus (l-pha), glycine max (sba), and triticum vulgare (wga) were fluoresceinated and incubated with living, ... | 1984 | 6609053 |
| lectin-binding and spontaneous capping characteristics of the thymocyte glycophorin-like glycoprotein. | monoclonal antibodies against lymphocyte glycoproteins have been used to identify the membrane molecules which bind peanut (pna) and helix pomatia (hpa) agglutinins and cap spontaneously on the uropod of polarized rat and mouse thymocytes. on the basis of co-capping experiments and radiolabelling of isolated glycoproteins after sodium dodecylsulfate polyacrylamide electrophoresis (sds-page), the major hpa- and pna-binding sialoglycoprotein (with an apparent molecular weight of about 105 k; (1k = ... | 1984 | 6609831 |
| cytochemical localization of terminal n-acetyl-d-galactosamine residues in cellular compartments of intestinal goblet cells: implications for the topology of o-glycosylation. | the o-linked oligosaccharides of mucin-type glycoproteins contain n-acetyl-d-galactosamine (galnac) that is not found in n-linked glycoproteins. because helix pomatia lectin interacts with terminal galnac, we used this lectin, bound to particles of colloidal gold, to localize such sugar residues in subcellular compartments of intestinal goblet cells. when thin sections of low temperature lowicryl k4m embedded duodenum or colon were incubated with helix pomatia lectin-gold complexes, no labeling ... | 1984 | 6693488 |
| human amyloid p component: a circulating lectin that modulates immunological responses. | amyloid p component (ap/sap), a glycoprotein, precipitated with purified snail galactans from helix pomatia and arianta arbustorum in a dose-dependent manner. radiolabelled ap binds to human peripheral blood mononuclear cells (pbmc), erythrocytes, and cells derived from human non-t, non-b acute lymphocytic leukaemia. the ap cell binding is specific in that it is dose-dependent and can be blocked both by excess cold ap and by helix pomatia galactan, although it cannot be blocked by an equal amoun ... | 1984 | 6710082 |
| detection of blood group a-like substance in bacterial and viral vaccines by countercurrent immunoelectrophoresis using helix pomatia lectin. | the technique of countercurrent immunoelectrophoresis (ci), using the n-acetyl glucosamine-binding lectin from helix pomatia, provided a rapid, sensitive, inexpensive, specific and reliable method for assaying blood group a-like substances in both bacterial and viral vaccines. blood group a-like substance was detected in the pneumococcal polysaccharide vaccine manufactured by merck sharp & dohme up to 1981 and in a staphylococcus vaccine ( staphage lysate) manufactured by delmont laboratories. o ... | 1984 | 6429147 |
| hla phenotype and insulin antibody production. | hla phenotypes have been determined in 79 patients as part of a prospective study of factors governing the immune response to injected insulin. igg insulin antibody levels 6 months after starting treatment with bovine insulin were significantly higher in patients bearing hla-dr7 and this in conjunction with the lack of a similar pattern in the igg response to helix pomatia haemocyanin, suggests the presence of an immune response gene for insulin. the hyporesponsiveness of hla-b8/dr3/c4aq0 positi ... | 1984 | 6432385 |
| appearance of helix pomatia lectin-binding sites on podocyte plasma membrane during glomerular differentiation. a quantitative analysis using the lectin-gold technique. | the visceral glomerular epithelial cells (podocytes) of newborn rat kidneys were examined by the lectin-gold technique to determine the distribution of helix pomatia lectin-binding sites during glomerular development. previous work has shown that this lectin, which primarily recognizes terminal n-acetyl-d-galactosamine residues of glycoconjugates (but also n-acetyl-d-glucosamine to a lesser extent), specifically labels the plasma membrane glycocalyx of podocyte foot process bases, whereas the pl ... | 1984 | 6471810 |
| binding of lectins to human mammary tumors: ultrastructural study. | the site of several lectin receptors in human mammary tumors and stroma was studied with the electron microscope. the advantage of the ultrastructural over light microscopic study was that lectin receptors could be localized with precision on the different cell types of the tumor and stroma. eighteen human mammary carcinomas were incubated with three peroxidase-labeled lectins: peanut agglutinin (pna), helix pomatia, and ulex europaeus i (uea). these lectins reacted in a selective way; some tumo ... | 1984 | 6487820 |
| enhanced metabolism of [2,4,6,7-3h] estradiol-17 beta in the diabetic rat. | the peripheral metabolism of [3h]estradiol-17 beta (e2(3)h) was studied in ovariectomized, streptozotocin induced diabetic rats, in an attempt to understand the reduced nuclear retention time and the decreased activity on protein synthesis of e2 in diabetic rat uterus as reported recently. e2(3)h was injected intraperitoneally and the kinetics of 3h metabolites were followed in plasma extracts obtained between the time intervals of 10-210 min after injection. diabetics accumulated about 15 times ... | 1984 | 6492801 |
| perturbation of podocyte plasma membrane domains in experimental nephrosis. a lectin-binding and freeze-fracture study. | alterations in the ultrastructural organization of podocyte plasma membrane domains were quantitatively assessed in puromycin aminonucleoside-treated rats by the use of 1) helix pomatia lectin-gold complexes for detection of a specific glycocalyx component(s) normally associated with foot process bases and 2) freeze-fracture for detection of intramembrane particles and endocytotic invaginations on the plasma membrane. lectin-binding sites were significantly reduced on podocyte foot process bases ... | 1984 | 6496656 |