Publications

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leishmania donovani: antibody response to chicken ovalbumin by infected golden hamsters. 19694188549
cellular immunity to leishmania donovani in macrophages in cultures. 19695764043
on the presence of multivesicular bodies in leishmania donovani. 19695800147
leishmaniasis in brazil. iv. the fox, cerdocyon thous (l) as a reservoir of leishmania donovani in para state, brazil. 19695368005
[study of the ultrastructure of leishmania donovani after numerous passages in vitro]. 19695375132
[ultrastructure of leishmania donovani (leptomonadic forms)]. 19695401156
[culture of 2 strains of leishmania donovani in a diphasic medium]. 19695409147
leishmaniasis in the sudan republic. 29. comparison and epidemiological implications of experimental canine infections with sudanese, mediterranean, and kenyan strains of leishmania donovani. 19705421087
ultrastructure of the promastigote of leishmania donovani. 19705439951
[the behavior of plasmodium vinckei in the mouse and its influencing by leishmania-donovani infection]. 19705473456
electronic cytochemical demonstration of a lysosome in leishmania donovani. 19705495632
experimental infection of animals with leptomonads of leishmania donovani. effect of cortisone on the infection in hamster and mouse. 19705508161
aldolase activity in the cell-free extract of the protozoan leishmania donovani. 19705512538
[studies on certain flagellata, their structure, function and other properties. ii. electron microscopic studies on the ultrastructure of leishmania donovani]. 19705526122
effect of macrophage engulfment and temperature on the transformation process of leishmania donovani. 19704195298
polyribosomes in thin sections of leishmania donovani. 19704392893
effects of interferon inducers on the intracellular growth of the protozoan parasite, leishmania donovani. 19704908372
interaction of leishmania donovani and hamster peritoneal macrophages. a phase-contrast microscopical study. 19714936115
inhibition of leishmania donovani reproduction during chronic infections in mice. 19715092414
occurrence of a 5,7-diene sterol in leishmania donovani. 19715093419
morphology and infectivity of leishmania donovani cultivated in nonliving media at elevated temperatures. 19715131691
macrophage activity of thymectomized mice infected with leishmania donovani. 19715132613
[effect of cortisone on experimental infections induced in hamsters and mice with leptomonad forms of leishmania donovani]. 19715152933
[flagellates, their structure, function and other properties. iv. effect of ginine and formaldehyde on the ultrastructure of leishmania donovani]. 19715152993
[flagellates, their structure, function and other properties. v. cytochemical studies of trypanosoma equiperdum and leishmania donovani]. 19715152994
leishmania donovani: assay for a functional pentose phosphate pathway. 19715545015
leishmania donovani; partial characterization of some flagellate cytoplasmic immunogens. 19725031323
a monophasic medium for cultivating leishmania donovani in large numbers. 19725057248
variation in susceptibility of mouse strains to leishmania donovani infection. 19725071050
preliminary electron microscope observations on the amastigote forms of trypanosoma cruzi and leishmania donovani. 19724558827
[common antigenic fractions revealed by immunoprecipitation in gel, between mycobacterium phlei (mycobacteriaceae) and leishmania donovani (trypanosomidae)]. 19724197659
appearance of leishmania donovani (kenya strain) in the blood of experimentally infected golden hamsters (mesocricetus auratus). 19724636103
proline metabolism in leishmania donovani promastigotes. 19724641905
[ecology of leishmaniasis in the south of france. 3. comparative receptivity of phlebotomus ariasi tonnoir 1921 and rhipicephalus turianicus pomerancev and matikasvili, 1940 to leishmania donovani (laveran and mesnil, 1903)]. 19724655366
interaction and transformation of leishmania donovani within in vitro cultured cells. an electron microscopical study. 19724673813
anaerobic formation of succinate from glucose and bicarbonate in resting cells of leishmania donovani. 19734691925
leishmania donovani: effect of temperature on rna metabolism. 19734691926
mystromys albicaudatus, the african white-tailed rat, as an experimental host for leishmania donovani. 19734760640
[characterization of leishmania donovani strains by disc electrophoresis]. 19734778589
[immunoelectrophoretic diagnosis of visceral leishmaniasis with the use of a hydrosoluble antigenic extract of leishmania donovani. preliminary results]. 19734198290
[studies on the detection of antibodies in kala-azar patients and animals experimentally infected with leishmania donovani (author's transl)]. 19734582782
evidence for a polysaccharide surface coat in the developmental stages of leishmania donovani: a fine structure-cytochemical study. 19744136730
[study on anti-leishmania donovani antibodies in a sample of animal serums]. 19744138212
cellular immunity to leishmania donovani. i. the effect of t cell depletion on resistance to l. donovani in mice. 19744214869
leishmania donovani and l. mexicana: production of the excretion factor. 19744372017
[comparative electrophoretic studies on leishmanial parasites of cutaneous leishmaniasis of the new world and their relationships to leishmania donovani and l. tropica (author's transl)]. 19744372753
effects of promastigote growth phase, frequency of subculture, and host age on promastigote-initiated infections with leishmania donovani in the golden hamster. 19744418716
studies on malate or oxaloacetate formation from bicarbonate and pyruvate or phosphoenol pyruvate in cell-free extracts of leishmania donovani. 19744452238
[protection against leishmania donovani induced in hamsters with an irradiated homologous strain]. 19744470177
cellular immunity to leishmania donovani. ii. evidence for synergy between thymocytes and lymph node cells in reconstitution of acquired resistance to l. donovani in mice. 19744547777
lectin binding saccharides on a parasitic protozoan.leishmania donovani promastigotes were specifically agglutinated by concanavalin a and phytohemagglutinin p. somatic-somatic, flagellar-somatic, and flagellar-flagellar type agglutination was observed with the lectins. enzyme-treated promastigotes gave reduced lectin agglutination reactions. the results suggest that complex saccharide moieties are randomly distributed on the surface of this organism.19744819678
[fractionation of bcg methylic extract and purification of the antigenic fractions related to leishmania donovani]. 19744845213
letter: genetic control of natural resistance to leishmania donovani. 19744852791
a serological study on human trypanosoma rhodesiense infections using a micro-scale enzyme linked immunosorbent assay.a micro-scale enzyme linked immunosorbent assay for african trypanosomiasis is described. the test uses very small quantities of sera and with t. brucei antigen is sensitive in detecting t. rhodesiense infections in man. cross reactions occur with antisera to, and antigens of, leishmania donovani and trypanosoma cruzi.19751099749
leishmania donovani: therapeutic and prophylaciic action of antimony dextran glycoside (rl-712) in the golden hamster. 19751126418
leishmania donovani: autoradiographic evidence for molecular exchanges between parasites and host cells. 19751126419
electrophoretic investigation of some dehydrogenases in leishmania amastigotes.amastigotes of leishmania mexicana mexicana and of leishmania donovani were grown in mouse peritoneal macrophages and dog sarcoma cells in culture. polyacrylamide gel disc electrophoresis of amastigote-infected tissue cultures failed to detect parasite lactate dehydrogenase and malate dehydrogenase, although the presence of glucose-6-phosphate dehydrogenase was indicated.19751155996
[ultrastructural observations on amastigote forms of leishmania donovani in a case of laryngeal leishmaniasis].an ultrastructural study on the amastigote stage of leishmania donovani, in a rare case of laringeal involvement, was carried out. many details of the fine morphology of this protozoon were observed under the electron microscope, i.e.: the outer membrane of host origin, periplast, subpellicular microtubules, kinetoplast, flagellum, flagellar pocket, multivesicular bodies. a presence of a new organelle (4 microtubules structure close to and running parallel to the flagellar pocket), previously no ...19751161176
liver-spleen scintiscan in kala-azar: case report.massive splenomegaly was found in a liver-spleen scan in a young man from greece. kala-azar was suggested as a possible cause and was confirmed by culturing leishmania donovani from the bone marrow aspirate. the differential diagnosis of massive splenomegaly should include kala-azar when a patient has been in an endemic area.19751194960
complement fixation and intradermal skin test with partially purified "proteic" and "polysaccharidic" antigens from leishmania donovani. 19751216300
[comparative electron microscope studies on the labelling leishmania donovani, leishmania tropica and leishmania braziliensis with ferritin (author's transl)].the labelling of negative charges on the cell surface of the developing promastigote forms in leishmania donovani, l. tropica and l. braziliensis was determined using cationized ferritin and electron microscopy. ferritin deposits were seen exclusively on the pellicle, since they cannot penetrate the cell membrane. the ferritin particles were distributed in regular rows covering the whole cell surface. the mean diameter of the particles was 8.76 nm and at a magnification of 104.000 an average of ...19751216327
polypeptide analysis of a "leishmania donovani" strain isolated during an outbreak of visceral leishmaniasis in northern italy.the results of the analysis of the polypeptide composition of a strain of leishmania donovani isolated during an outbreak of kala azar in northern italy are reported.19751231653
comparison of two geographic strains of leishmania donovani by resistance of mice to superinfection.groups of mice were injected with leishmania donovani strains et (burma) or is (sudan) and the courses of infection were followed. between days 20 and 30 after infection by treatment with pentostam. et- and 1s-recovered mice were challenged by intravenous injection of amastigotes of each strain. although resistance to superinfection was apparent the l. donovani strains could not be differentiated by this method.1975125553
[immunocyte adherence and leukocyte migration inhibition tests in dogs infested with leishmania donovani. importance of lymphocyte conservation in liquid nitrogen].a method of canine lymphocytes isolation and storage in liquid azote with dmso is described. it was possible to use these lymphocytes in the immunocytoadherence and leucocytes migration inhibition tests in dogs infected with leishmania donovani. the sensitisation was made with a soluble antigen from promastigotes of l. donovani braid by ultrasounds. the dogs with leishmaniasis gave positive results. the controls without leishmaniasis were negative. the tests with conserved lymphocytes were as sa ...1975126717
a simple monophasic medium for axenic culture of hemoflagellates.an inexpensive, easily prepared monophasic medium which supports continuous culture of leishmania donovanì (approximately 4 x 10(7) cells/ml/day 5) and l. tarentolae (approximately 3.5 x 10(7) cells/ml/day 5) promastigotes has been developed. this medium, designated ho-mem, is a modified eagle's minimal essential medium with spinner's salts and 10% fetal calf serum. epimastigotes of trypanosoma cruzi, costa rica strain, also grew well in ho-mem (approximately 2 x 10(7) cells/ml/day 5) but cells ...1976778371
antibody-induced modulation of leishmania donovani surface membrane antigens.the effects of specific antibodies on the surface membrane antigens of leishmania donovani (strain 1s, clone 2d) amastigote and promastigote developmental stages was detected in vitro by using several direct and indirect immunofluorescence methods. stage specific anti-l. donovani sera and sera from l. donovani-infected hamsters induced parasite surface membrane antigens to aggregate, move along the longitudinal cell axis, form polar cell caps, and subsequently disappear. no membrane fluorescence ...1976792338
leishmania donovani multiplication in a cell line of aedes albopictus. 1976841664
infectivity of leishmania donovani amastigotes and promastigotes for golden hamsters.intracardial injection of hamsters with from 5 to 114 million amastigotes or promastigotes of leishmania donovani and screening of the 8th-day liver impression smears, provides a rapid and reproducible method for assaying infectivity. amastigotes are at least 10x more infective than promastigotes, and log-phase promastigotes act as a single infective population for hamsters.1976933082
multiplication of a human parasite (leishmania donovani) in phagolysosomes of hamster macrophages in vitro.leishmania donovani, the etiological agent of human visceral leishmaniasis, was grown in hamster peritoneal macrophages in vitro. by electron microscopy, using a lysosomal marker, these parasitic protozoa were seen to multiply within host cell phagolysosomes. the survival mechanism of this intracellular parasite is based apparently upon resistance to macrophage lysosomal enzymic digestion.1976948742
leishmania donovani: acquired resistance to visceral leishmaniasis in the golden hamster. 1976950004
inhibition of leishmania donovani transformation by hamster spleen homogenates and active human lymphocytes. 1976958439
activities of glucose-6-phosphate, 6-phosphogluconate, and isocitrate dehydrogenases from leishmania donovani cultivated at 25 and 37 c.the activities of glucose-6-phosphate dehydrogenase (g-6-pd) (ec no. 1.1.1.49), 6-phosphogluconate dehydrogenase (pgd) (ec no. 1.1.1.44), and isocitrate dehydrogenase (icd) (ec no. 1.1.1.42) from promastigotes of leishmania donovani strain 3s grown at 25 c in modified tobie's (mt) medium and from promastigotes of the 37 c-adapted substrain of this strain cultivated in the mt at 37 c were assayed at 25 and 37 c. at 25 c icd from both the strain and the substrain had the highest, and pgd, the lowe ...1976972354
a defined medium for cultivating leishmania donovani and l. braziliensis. 19761003271
quantitative ultrastructural investigations of mitochondrial development in leishmania donovani during transformation.the ultrastructure of the mitochondrion during transformation of leishmania donovani from amastigote to promastigote stages was studied by morphometric analysis. there was a slight but significant decrease in relative mitochondrial volume (vvmi) during transformation. this decrease was linear with time for at least 27 hr. no change in relative lipid inclusion volume (vvli) was observed during transformation. the ratio of inner to outer mitochondrial surface membrane densities (svmii:svmio) also ...19761003340
antileishmanial activity of mycobacillin.mycobacillin, a cyclic polypeptide antifungal antibiotic effectively inhibits the growth of promastigote form of the protozoal organism, leishmania donovani, strain 81 in liquid medium. oxygen uptake by intact cells of the protozoa with exogenous glucose is appreciably reduced within first 30 minutes in presence of the antibiotic at a concentration of 15 microng/ml. appreciable leakage of intracellular 260 nm and 280 nm absorbing materials takes place from the protozoan cells incubated with the ...1977863786
effect of treatment with bcg on the course of visceral leishmaniasis in balb/c mice.intravenous inoculation of bcg was found to be both prophylactic and therapeutic in balb/c mice against challenge with amastigotes of leishmania donovani. spleens and livers of mice inoculated with bcg maintained total parasite burdens at significantly lower levels when compared to controls. bcg administered intravenously 14 days prior to and on the same day of protozoan challenge was more protective than vaccine given 30 and 14 days prior to challenge. a level of 10(7) viable units of bcg provi ...1977873609
[atypical course of leishmania donovani infections in dogs. histopathological findings (author's transl)].four beagles infected with an indian strain of leishmania donovani showed histiocytic granulomas with very few leishmania in liver and skin biopsies. 3 animals died with formation of tumor-like histiocytic infiltrates in several organs (heart, ling, liver, kidney, pancreas, muscle) and with development of anyloidosis; one survived with minimal residual lesions.1977888184
cultivation of leishmania donovani and leishmania braziliensis in defined media: nutritional requirements.nutritional requirements of promastigotes of leishmania donovani and leishmania braziliensis were studied in modifications of a simple defined culture medium. "continuous growth," considered as propagation through 10 successive passages, was supported by inorganic salts, 14 l-amino acids (arginine, cysteine, glutamine, histidine, isoleucine, leucine, lysine, methionine, phenylalanine, proline, threonine, tryptophan, tyrosine, valine), glucose, adenosine, and a mixture of 11 vitamins and related ...1977915847
survival of aegyptianella pullorum, anaplasma marginale and various parasitic protozoa following prolonged storage in liquid nitrogen.various protozoa species were examined using in vivo and in vitro methods to determine their ability to survive prolonged periods of storage in liquid nitrogen. the following protozoan species were successfully recovered after they had been cryopreserved for a period over 10 years: trypanosoma lewisi, t. cruzi, t. congolense, t. brucei, t. rhodesiense, t. gambiense, t. evansi, t. equinum, t. equiperdum, leishmania donovani, plasmodium berghei, p. praecox (relictum), babesia rodhaini and b. canis ...1977919686
antileishmanial effect of allopurinol. ii. relationship of adenine metabolism in leishmania species to the action of allopurinol.allopurinol was shown to be effective in vitro against leishmania mexicana and leishmania donovani as well as against leishmania braziliensis. the major metabolic derivative of allopurinol in humans, oxipurinol, also is antileishmanial for l. donovani. the antileishmanial effect of allopurinol and oxipurinol can be specifically reversed by adenine, and its metabolic precursors and derivatives, but by no other purines or their derivative. it is proposed that the adenylosuccinate synthetase or the ...1977925380
leishmania donovani: surface membrane carbohydrates of promastigotes. 1977557418
ultrastructural localization of diaminobenzidine reactivity in leishmania donovani promastigotes.the ultrastructural localization of the activities of two enzyme systems in the culture forms of leishmania donovani was shown by means of the diaminobenzidine techniques. the consistent deposition of electron dense reaction product of dab oxidation without h2o2 in the kinetoplast and mitochondrial cristae and membranes was taken as evidence of the presence of cytochrome oxidase activity and cytochrome c. in the presence of h2o2, a more intense dab oxidation was attributed to the activity of a p ...1977198923
amino-acid and glucose utilization of leishmania donovani and l. braziliensis.the availability of a defined medium (steiger & steiger, 1976) has enabled studies on the nutritional requirements of promastigotes of l. donovani and l. braziliensis (steiger & steiger, 1977). as outlined elsewhere (steiger & meshnick, 1977), the present paper describes preliminary experiments dealing with the utilization of amino-acids and glucose by these two leishmania species. the data are compared to those obtained with trypanosoma (cross et al., 1975a,b; brun & jenni, 1977) and are discus ...1977595100
testing of drugs for antileishmanial activity in golden hamsters infected with leishmania donovani. 1977598961
regulation of leishmania populations within the host. i. the variable course of leishmania donovani infections in mice.the course of infection with leishmania donovani was followed in seven strains of laboratory mice with measurement of the liver parasite burdens over 20 weeks. the acute parasite population growth rate varied greatly between, but not within, strains. four strains were relatively resistant with less than an eight-fold increase while the three acutely susceptible strains showed over an eight-fold increase in the first month. thereafter, one initially susceptible strain showed a dramatic fall in pa ...1977606433
regulation of leishmania populations within the host. ii. genetic control of acute susceptibility of mice to leishmania donovani infection.the acute growth rates of leishmania donovani populations in twenty-five inbred mouse strains fall into two distinct groups: the susceptible (s) and the resistant (r). hybrids within either category resemble their parents in susceptibility. hybrids between categories are moderately resistant. back-crossing of f1 hybrids to r and s parents, and interbreeding the f1 generation, give susceptibility ratios consistent with single gene control of acute susceptibility to visceral leishmaniasis. the dis ...1977606434
in vitro efficacy of some drugs on promastigotes of leishmania donovani.sodium stibogluconate, metronidazole and dehydroemetine roche were tested for their in vitro action on the mobility, morphology and survival of promastigotes of leishmania donovani. at low concentrations, sodium stibogluconate, the drug of choice for kala azar treatment in iraq proved to be poorly effective in the prevention of promastigotes growth in vitro. on the other hand, both metronidazole and dehydroemetine roche at low concentrations were highly effective in the prevention of promastigo ...197716732
phosphofructokinase of leishmania donovani and leishmania braziliensis and its role in glycolysis.it was shown in an investigation of the phosphofructokinases of leishmania donovani and leishmania braziliensis that both enzymes are similar to that of crithidia fasciculata. although the enzymes are allosteric with respect to their substrates and require amp for activation, there is no influence by other heterotropic modifiers. the mg2+-atp chelate activates these enzymes in a first order process and they can be inhibited by free atp. the inhibition is reversed by the activator, amp, in a comp ...197718601
the effect of 4-nitroquinoline 1- oxide on macromolecular synthesis in leishmania donovani promastigotes. 197720270
leishmania donovani and l. braziliensis: function of pyruvate kinase. 197723305
immunofluorescence and counter immunoelectrophoresis in the diagnosis of kala-azar.techniques of indirect immunofluorescence (if) and counter current immunoelectrophoresis (ciep) were employed and compared for the diagnosis of kala-azar among children in fars province of iran. it is suggested that the diagnostic titre for kala-azar by immuno fluorescence is over 1 in 64. in fact a majority of the cases had a titre of more than 1 in 256. ciep using soluble antigens prepared form the promastigote form of leishmania donovani, was also applied to sera from patients with systemic l ...1977327629
antigenic cross-reactivity between mycobacterium bovis (bcg) and leishmania donovani.antigenic cross-reactivity was demonstrated between mycobacterium bovis (bcg) and leishmania donovani, using delayed hypersensitivity as a criterion.1977336551
glycoproteins released by leishmania donovani: immunologic relationships with host and bacterial antigens and preliminary biochemical analysis.the antigenically active glycoproteins (aagp) released by leishmania donovani strain 3s promatigotes into growth media and by amastigotes of this strain into the tissue, e.g. blood, of infected hamsters was found to consist of 6 to 7 antigenically distinct components. the antigenic activity of these glycoproteins was resistant to freeze-thawing, protease treatment, and purification by column chromatography using sephadex g-100. this activity, however, was destroyed by na periodate and altered by ...197884074
synthesis of 2-substituted primaquine analogues as potential antimalarials.a series of 2-substituted primaquine analogues has been synthesized and evaluated against plasmodium berghei in the mouse and leishmania donovani in the hamster. three members (3a,d,e) of the series were evaluated against plasmodium cynomolgi in the rhesus monkey. one analogue (3d) was evaluated against trypanosoma rhodesiense in the mouse, and two (3b,e) were evaluated against schistosoma mansoni in the mouse. several analogues possessed significant activity against p. berghei (3e,f) and l. don ...1978102798
an easily prepared defined medium for cultivation of leishmania donovani promastigotes. 1978627959
sequence arrangement of the 16s and 26s rrna genes in the pathogenic haemoflagellate leishmania donovani.kinetic and chemical analysis show that the haploid genome of leishmania donovani has between 4.6 and 6.5 x 10(7) kb pairs of dna. cot analysis shows that the genome contains 12% rapidly reassociating dna, u3% middle repetitive dna with an average reiteration frequency of 77 and 62% single copy dna. saturation hybridization experiments show that 0.82% of the nuclear dna is occupied by rrna coding sequences. the average repetition frequency of these sequences is determined to be 166. sedimentatio ...1978634795
visceral leishmaniasis masquerading as a nasopharyngeal tumour. report of a case.a 55-year-old male, a resident of a village in the foothills of the himalayas in the north-western region of india, presented with a huge nasopharyngeal tumour but was subsequently found to be infected with leishmania donovani, involving the nasopharyngeal tissue and the draining lymph nodes as well as a visceral infection.1978635975
the antileishmanial activity of lepidines.a series of lepidines (6-methoxy-4-methyl-8-aminoquinoline derivatives) was studied in a hamster-leishmania donovani model. members of this class were found to have activity many-fold that of the standard, meglumine antimoniate (glucantime). one of them, 8-(6-diethylamino-hexylamino)-6-methoxy-4-methylquinoline, designated wr 6026, when given orally was over 700 times as effective as the standard antimonial drug.1978686239
[immunopathology in a case of kala-azar (proceedings)].a 33 year old woman was admitted to hospital for fever of unknown origin. leishmania donovani was found in histological preparations from lymphnodes and by sternal puncture. circulating immune complexes present in high concentration were isolated and characterized. the circulating immune complexes remained elevated two months after the disappearance of leishmania from the bone marrow.1978694498
exometabolites of leishmania donovani promastigotes. i. isolation and initial characterization. 1978714924
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