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yeast-suspension as soiling matter in disinfectant testing.using the kelsey-sykes capacity-test, it was found that a sterile yeast suspension used to simulate 'dirty' conditions, gave an increased effect of chloramine t against the fungi candida albicans, aspergillus fumigatus, geotrichum candidum and penicillium sp. compared with the effect under 'clean' conditions. this effect was not found with the fungus rhodotorula rubra nor on the various bacteria tested. the enhanced effect was found with respect to both chloramine t and chloramine b, but not wit ...19892661973
biotransformation and detoxification of t-2 toxin by soil and freshwater bacteria.bacterial communities isolated from 17 of 20 samples of soils and waters with widely diverse geographical origins utilized t-2 toxin as a sole source of carbon and energy for growth. these isolates readily detoxified t-2 toxin as assessed by a rhodotorula rubra bioassay. the major degradation pathway of t-2 toxin in the majority of isolates involved side chain cleavage of acetyl moieties to produce ht-2 toxin and t-2 triol. a minor degradation pathway of t-2 toxin that involved conversion to neo ...19892705769
pseudoepidemic of rhodotorula rubra in patients undergoing fiberoptic bronchoscopy.between march and june 1988, rhodotorula rubra was isolated from the bronchial washings of 30 of 56 (54%) patients undergoing bronchoscopy at a north carolina community hospital. pulmonary disease consistent with invasive fungal pneumonia was not apparent for any patient. repeat sputum cultures were performed on 11 patients, none of whom were positive for r rubra. investigation revealed fungal contamination of two brushes used to clean the bronchoscope channels and one positive sample of the tub ...19892584679
growth of opportunistic yeasts on vitamin-free solid medium.the growth of some opportunistic yeasts (candida albicans, cryptococcus neoformans, torulopsis glabrata, rhodotorula rubra, geotrichum candidum, trichosporon cutaneum, saccharomyces cerevisiae) was essayed in a solid minimal synthetic medium without vitamins. from the preliminary results some species (candida albicans, cryptococcus neoformans, rhodotorula rubra, geotrichum candidum and saccharomyces cerevisiae) seem to be autotrophic regarding the growth factor considered indispensable for their ...19902191196
oral yeasts in patients with cancer of the mouth, before and during radiotherapy.the yeasts of patients with oral cancer has been studied before and during xr-therapy. gram and pas smears revealed an increase of yeast-like structures, during treatment, from 56% to 66% of the cases. before radiotherapy oral yeasts were isolated from 56% of the patients with cancer represented by candida albicans (30%); c. tropicalis (12%); c. glabrata and c. krusei (4%), besides six other different species (2%). during radiotherapy yeasts were isolated in 72% of the cases, as follow: c. albic ...19902293033
yeasts and fungi occurring in ensiled whole-crop maize and other ensiled vegetable crops.the yeast flora of whole-crop maize ensiled for two weeks was predominated by candida holmii, c. lambica, c. milleri, hansenula anomala and saccharomyces dairensis. inoculation with other yeast species reported in the literature to prevail in maize or wheat silages did not alter the yeast flora. at 25 or 30 degrees c the ascomycetous fermentative species found at 20 degrees c were accompanied with ascomycetous non-fermentative fungi, i.c. exophiala jeanselmei and verticillium psalliotae, by the ...19902321936
pseudoepidemic of rhodotorula rubra in patients undergoing fiberoptic bronchoscopy. 19902115899
[changes in the mutagenic effect of a medium containing aflatoxin b1 as a result of the yeast activity].changes in the mutagenic activity of the nutrient medium containing aflatoxin br as a result of living activity of the yeast rhodotorula mucilaginosa i b has been studied. reduction of mutagenic activity of the medium when tested on the strains salmonella typhimurium ta98 (6.3 times fold), ta100 (2.2 times fold), ag262 (2 times fold) has been revealed. the data obtained leads to the conclusion that the technology, based on the use of the indicated strain of the yeast for the treatment of food an ...19902128746
silver tolerance and accumulation in yeasts.debaryomyces hansenii (ncyc 459 and strain 75-21), candida albicans (3153a), saccharomyces cerevisiae (x2180-1b), rhodotorula rubra (ncyc 797) and aureobasidium pullulans (imi 45533 and atcc 42371) were grown on solid medium supplemented with varying concentrations of agno3. although ag+ is highly toxic towards yeasts, growth on solid media was still possible at ag concentrations of 1-2 mm. further subculture on higher ag concentrations (up to 5 mm) resulted in elevated tolerance. the extent of ...19911878280
analysis of the gene for phenylalanine ammonia-lyase from rhodosporidium toruloides.we have cloned and sequenced the pal gene encoding phenylalanine ammonia-lyase (pal) from rhodosporidium toruloides strain cbs14. our data imply a different start codon and thus a different amino acid sequence for the n-terminus of pal as compared to the previously published sequence for pal from r. toruloides strain if00559. primer extension analysis shows three transcription initiation sites with non-translated leaders of 24-35 nucleotides. upstream of these initiation sites is a long stretch ...19911773059
cytochrome p-450-dependent catabolism of triethanolamine in rhodotorula mucilaginosa.the yeast rhodotorula mucilaginosa was able to grow in media containing triethanolamine or diethanolamine as the sole nitrogen source. during growth in the presence of triethanolamine, extracts of yeast cells contained increased levels of cytochrome p-450 dependent monooxygenase which catalyzed the oxidative n-dealkylation of aminoalcohols. formation of diethanolamine, ethanolamine and glyoxylate from triethanolamine was demonstrated, and the identity of the products was verified by thin layer c ...19911368151
[biological features and experimental pathogenicity of candida strains isolated by hemoculture at the hospital infantil de méxico "federico gómez"].in a period of 15 months (1990-1991) it was carried out 5781 blood cultures in which 180 strains of yeast-like were isolated. 116 of these strains were selected for biochemical classification, cellular and colonial morphology and experimental determination of virulence in mice. most of the strains were classified as candida albicans (60%) and the others were c. tropicalis (15.5%); c. guillermondii (10.3%); t. glabrata (6.8%); rhodotorula rubra (3.4%); cryptococcus neoformans (2.58%) y c. parapsi ...19921345115
rhodotorula fungaemia: a life-threatening complication of indwelling central venous catheters.a 30-year-old woman receiving total parenteral nutrition via an indwelling central venous catheter for an intestinal motility disorder developed fever, tachycardia, tachypnea, and hypotension. multiple blood cultures drawn through the catheter prior to these events, as well as a peripheral blood culture obtained earlier, grew the red yeast rhodotorula rubra. the patient was critically ill for over one month but eventually recovered with therapy including the systemic antifungal agents amphoteric ...19921302803
characterization of trehalase in rhodotorula rubra.trehalase activity in rhodotorula rubra was found to be bound to the particulate fraction of a cell-free extract in contrast with the soluble trehalase activity of saccharomyces cerevisiae. the enzyme was strongly repressed by glucose and derepressed during growth on maltose, trehalose and glycerol. this increase in activity was due to a "de novo" synthesis as seen by inhibition with cycloheximide, a mechanism not described for saccharomyces cerevisiae. catabolite inactivation by addition of glu ...19921482405
causes of conductance change in yeast cultures.the conductance change due to growth of saccharomyces cerevisiae y112, zygosaccharomyces bailii m and rhodotorula rubra ncyc 63 in culture media containing glucose, tartrate ph buffer and ammonium ions as sole nitrogen source was compared with that in a medium containing l-asparagine as sole nitrogen source. decreases in conductance were observed in glucose-ammonium cultures of all three yeasts while little change occurred in cultures with l-asparagine as sole nitrogen source. this supports the ...19921541598
rhodotorula rubra contamination in fiberoptic bronchoscopy.rhodotorula rubra was recovered in 18 bronchoscopic specimens from 15 patients from may to november 1987. one hundred and twenty-one bronchoscopies were performed during that period by two bronchoscopists (w. w.; r.d.) at letterman army medical center in san francisco. isolation of r rubra occurred in 11 bronchoalveolar lavage (bal) specimens, four bronchial washes, and three transbronchial biopsies. clinical infection was not present in any of these patients, although five were immunocompromise ...19921424874
evaluation of the pro-lab id ring system for the identification of medically important yeasts.we evaluated 151 coded isolates of medically important yeast species belonging to the genera candida, cryptococcus, geotrichum, rhodoturula, saccharomyces and torulopsis using the newly developed rapid pro-lab identification ring, pl 960 system (plid-ring). all isolates were concurrently identified by the api 20c and conventional procedures comprising macro- and micromorphology, assimilation and fermentation of various carbon and nitrogen compounds. the plid-ring system identified isolates of ca ...19921406902
[fungemia caused by rhodotorula mucilaginosa in relation to total parenteral nutrition].fungal infections are nowadays more common in clinical practice. the most frequently isolated fungi are candida and cryptococcus. infection due to rhodotorula mucilaginosa is very uncommon. we describe here our experience with r. mucilaginosa fungemia, with emphasis on total parenteral nutrition related episodes.19921489796
[limulus test (factor g) and polysaccharides from fungus].picogram quantifies per ml of endotoxin showed a positive limulus test. the concentration more than ten pg/ml of curdlan ((1-3)-beta-d-glucan) was also positive to a conventional limulus test, which contains a factor g. one nanogram per ml of cm-curdlan was positive, but higher concentration of 10 pg/ml of cm-curdlan decreased its optical density of the conventional limulus test. more than one hundred ng/ml of mannan from saccharomyces cerevisiae did not react with factor g. however, a culture s ...19921402105
extrinsic allergic alveolitis after exposure to the yeast rhodotorula rubra.a case of extrinsic allergic alveolitis following exposure to the red yeast rhodotorula rubra is reported--to our knowledge, for the first time. extensive growth of the yeast in the patient's environment was demonstrated, explaining an elevated titer of rhodotorula-specific precipitating antibodies in his serum. a bronchial provocation test confirmed the diagnosis.19938328668
the expression of potential colonization factors of yeasts isolated from fish during different growth conditions.three strains, rhodotorula rubra, rhodotorula glutinis, and candida zeylanoides, isolated from fish, were tested for the expression of putative tissue colonization factors. all strains were able to bind collagen type i, fibronectin, and laminin to various degrees after growing on various solid and broth media, while the binding to collagen type iv was sparse under all conditions tested. for the three strains tested, a very low cell surface hydrophobicity was shown for growth on various solid and ...19938131110
mechanisms of ferulic acid conversions to vanillic acid and guaiacol by rhodotorula rubra.resting cells of rhodotorula rubra converted transferulic acid (1) to vanillic acid (2), then to guaiacol (3) and protocatechuic acid (4), under aerobic conditions. in an argon atmosphere, r. rubra transformed ferulic acid to vanillic acid and 4-hydroxy-3-methoxystyrene (5). metabolites were isolated by solid-phase extraction and characterized by mass spectrometry, 1h, and 13c-nuclear magnetic resonance spectroscopy (nmr). the biotransformation of ferulic acid to vanillic acid by r. rubra cell-f ...19938226936
new cause for false-positive results with the pastorex aspergillus antigen latex agglutination test.the pastorex aspergillus antigen test for detection of aspergillus galactomannan antigen in the sera of patients with invasive aspergillosis is used in many clinical laboratories. a serum sample contaminated with penicillium chrysogenum gave a strongly positive reaction (1:128) which was heat stable, was not eliminated by pronase treatment, and was not detected by a normal rabbit globulin control. this observation was shown to be due to cross-reactions of the monoclonal antibody eb-a2 used by th ...19938408572
biotransformation--xxxiv. metabolism of testosterone esters in fungi cultures.seven esters of testosterone: acetate, propionate, enanthate, caprate, undecanoate, isobutyrate and isocaproate (some of them are used as drugs) were transformed by microorganisms: absidia coerulea, acremonium roseum, aphanocladium album and rhodotorula mucilaginosa to obtain some information about their metabolism. it was observed that the presence and structure of the acyl group mainly influenced the degree of transformation. the first step of the reaction was probably hydrolysis of ester, fol ...19938664175
evaluation of two commercialized systems for the rapid identification of medically important yeasts.a total of 77 recent clinical isolates of candida albicans and other medically important yeasts were identified by two different commercial tests, rapidec albicans (api-biomérieux) and fongiscreen 4h (sanofi diagnostics pasteur), and conventional mycological methods. the strains were from 13 different species of yeasts and consisted of strains of 36 c. albicans, three of candida famata, nine of candida (torulopsis) glabrata, five of candida guilliermondii, two of candida kefyr, three of candida ...19938015561
disseminated rhodotorula rubra infection in an hiv-infected drug abuser. 19938261084
fungal esophagitis in children.seven patients were endoscopically diagnosed as having a fungal esophagitis with mycologic or histologic support for the diagnosis from 1979 to 1991 in the department of pediatrics, national taiwan university hospital. the major causative agent was candida albicans. other fungi isolated were candida krusei, trichosporon cutaneum, trichosporon beigelii, and rhodotorula rubra, but they all resembled one another under endoscopic examination. the most common presenting symptom was hematemesis, and t ...19938296555
rapid identification of yeast species using three primers in a polymerase chain reaction.classic methods for identification of yeasts rely on a variety of morphological and physiological tests that often take days to weeks to complete. we have been able to reduce the time to less than one day through the use of multiple segment-specific oligonucleotide priming of a region of the large subunit rdna in a polymerase chain reaction. the "hot start" reaction was used with two universal external delimiting primers and one internal species-specific primer. five specific primers were tested ...19938364695
[the effect of lovastatin on sterol synthesis and yeast resistance to polyene antibiotics].lovastatin (monocolin k) is a competitive inhibitor of 3-hydroxy-3-methylglutaryl-coa-reductase. its influence on the growth of a lovastatin sensitive strain of rhodotorula rubra, the biosynthesis of ergosterol and the resistance to polyenic antibiotics was studied. it was shown that the lovastatin action on the strain depended on the inhibitor dose. in a concentration of 0.1 to 0.5 micrograms/ml lovastatin inhibited the yeast growth and ergosterol biosynthesis. higher concentrations of the inhi ...19938085886
nadh: ubiquinone oxidoreductase in obligate aerobic yeasts.the strictly aerobic yeasts candida pinus, cryptococcus albidus, rhodotorula minuta, rhodotorula mucilaginosa and trichosporon beigelii possess mitochondrial nadh dehydrogenases with significant features of the nadh:ubiquinone oxidoreductase (complex i). these species show in all growth phases and under standard cultivation conditions, nadh dehydrogenases of approximately 700 kda, which are sensitive to rotenone, a specific inhibitor of this complex. identical results were obtained with the weak ...19947941733
relevance of chlorine-substituent for the antifungal activity of syringomycin and syringotoxin, metabolites of the phytopathogenic bacterium pseudomonas syringae pv. syringae.structural analogues of syringomycin and syringotoxin were produced by fermentation, characterized by fab-ms and amino acid analysis and compared to the parent compounds in the antibiosis test against rhodotorula pilimanae. the c-terminal residue was shown to be important for the activity.19948125171
response of bacteria and fungi to high-pressure stress as investigated by two-dimensional polyacrylamide gel electrophoresis.in an attempt to generalize previous observations (jaenicke et al., appl. environ. microbiol. 1988, 54, 2375-2380) and to find a convenient model system for studies of the pressure response, we tested the suitability of escherichia coli and thermotoga maritima (bacteria), and of five different eukaryotic species including the filamentous fungi asteromyces cruciatus and dendryphiella salina, and the marine yeasts debaryomyces hansenii, rhodosporidium sphaerocarpum, and rhodotorula rubra. using tw ...19947720693
microbial flora and some chemical properties of ersho, a starter for teff (eragrostis tef) fermentation.ersho is a clear, yellow liquid that accumulates on the surface of fermenting teff-flour batter and is collected to serve as an inoculum for the next fermentation. the ph of ersho samples was about 3.5 and titratable acidity ranged between 3.1% and 5.7%. the mean aerobic mesophilic counts from four households varied between 6.9×10(6) and 1.3×10(8) c.f.u./ml and the aerobic bacterial flora consisted of bacillus spp. mean yeast counts ranged between 5.2×10(5) and 1.8×10(6) c.f.u./ml and comprised, ...199424420890
yeast colonizing the intestine of rainbow trout (salmo gairdneri) and turbot (scophtalmus maximus).yeast were isolated from the intestine of farmed rainbow trout (salmo gairdneri), turbot (scophtalmus maximus), and free-living flat-fish (pleuronectes platessa and p. flesus). the average number of viable yeasts recovered from farmed rainbow trout was 3.0 × 10(3) and 0.5 × 10(2) cells per gram homogenized intestine for white and red-pigmented yeasts, respectively. the dominant species were debaryomyces hansenii, saccharomyces cerevisiae, rhodotorula rubra, and r. glutinis. in 5 of 10 free-lving ...199524185568
utilization of ammonia, generated from abiotic cyanide degradation, by rhodotorula rubra.the viability of the yeast rhodotorula rubra, isolated from liquid samples of gold-mine effluents, was not affected by the presence of 11.52 mm cyanide. the yeast was able to utilize ammonia, generated from abiotic cyanide degradation in the presence of reducing sugars, in aerobic culture at ph 9.0. these physiological characteristics encourage studies with mixed cultures of cyanide-degrading organisms, using this yeast as an assimilator of ammonia.199524414663
rdna targeted oligonucleotide primers for the identification of pathogenic yeasts in a polymerase chain reaction.species-specific oligonucleotide primers were designed for pcr identification of the basidiomycetous yeasts cryptococcus neoformans, trichosporon cutaneum and rhodotorula mucilaginosa. the procedure uses standard pcr components including dna from the test species and three primers: two universal external (upstream and downstream) limiting primers and a species-specific internal primer. species identification requires the formation of a species-specific rdna nucleotide segment that is significant ...19957662289
rhodotorula rubra peritonitis in an hiv+ patient on capd. 19957705519
fungemia in children infected with the human immunodeficiency virus: new epidemiologic patterns, emerging pathogens, and improved outcome with antifungal therapy.we characterized 27 episodes of fungemia in 22 children infected with the human immunodeficiency virus (hiv). fungemia in these patients presented as a community-acquired infection in the setting of outpatient total parenteral nutrition or intravenous antibiotic therapy through a chronically indwelling central venous catheter (cvc). fungemia developed only in patients with cvcs (p < .001). non-albicans candida species, torulopsis glabrata, rhodotorula rubra, and bipolaris spicifera constituted 5 ...19957795092
(1-->3)-beta-d-glucan in culture fluid of fungi activates factor g, a limulus coagulation factor.two well-known polysaccharides, (1-->3)-beta-d-glucan and mannan, are major structural components of the fungus cell wall. the g test is a direct method to measure (1-->3)-beta-d-glucan using a (1-->3)-beta-d-glucan-sensitive component, factor g, fractionated from the limulus lysate. the concentration of (1-->3)-beta-d-glucan in culture supernatants of candida albicans increased to 1,390.0 pg/ml at 24 hours. the concentration of mannan also increased parallel with fungal growth. however, after d ...19958531015
[skin test with aeroallergens untested in mexico].this study was carried out to prove that some molds even not studied in mexico can be able to incit a positive skin test. we tested 43 adults (15-45 years old), 34 of them suffering respiratory allergy verified by roentgenography and laboratory tests including traditional intradermic allergic test. 9 subjects served as control group. we used manual scarification to ustilago maydis, rhodotorula rubra, puccinia graminis, cunhigamella sp and zyncephalastrum sp. our patients showed a positive skin t ...19958581450
fungal infections in neutropenic patients. a 8-year prospective study.in this paper we report a eight-year prospective study designed to further characterize incidence, epidemiology, specific syndromes, treatment and prognosis associated with fungal infections in neutropenic patients. during the study period 30 fungal infections were diagnosed in 30 patients among 313 episodes of fever and neutropenia (10%). there were 15 cases of candidiasis, 5 pulmonary aspergillosis, 3 sinusitis by aspergillus fumigatus, 5 infections by fusarium sp., one infection by trichospor ...19958729749
[the clinical picture of mycotic complications in hiv-infected patients].mycotic complications were registered in 21 out of 37 hiv-infected subjects. oropharyngeal candidiasis was most common. it occurred prior to or concurrently with esophageal and skin candidiasis, fungemia, meningoencephalitis and disseminated lesions. with immunodeficiency progression, the prevalence and severity of mycosis go up. the causing fungi vary in great range: candida albicans, candida krusei. candida tropicalis, candida pseudotropicalis, candida parapsilosis. cryptococcus neoformans, rh ...19958577107
a pseudoepidemic of rhodotorula rubra: a marker for microbial contamination of the bronchoscope.rhodotorula rubra was isolated from bronchoscopy specimens from 11 patients. an investigation of the bronchoscopy equipment and the bronchoscopy suite revealed contamination of the suction channel with r rubra, as well as potentially pathogenic bacteria. disinfection control methods included gas sterilization of the bronchoscope and the institution of an alcohol and air flush through the suction channel to allow complete drying of the scope between each patient use. we have had no further isolat ...19958683092
evaluation of a commercial medium for identification of candida species.chromagar candida (chromagar, france) was evaluated as a medium for the presumptive identification and isolation of yeasts using 1,537 isolates of medically important yeasts, including 970 candida albicans, 165 candida parapsilosis, 131 candida glabrata, 62 candida guilliermondii, 35 candida krusei, 32 candida tropicalis, 31 rhodotorula rubra, 23 trichosporon spp. (17 trichosporon beigelii), 17 candida famata, 16 candida pelliculosa, 10 pichia etchelsii, 10 saccharomyces cerevisiae, 8 candida lu ...19968801088
ability of rapid yeast plus system to identify 304 clinically significant yeasts within 5 hours.the rapid yeast plus system (innovative diagnostic systems, norcross, ga.) is a qualitative micromethod that uses conventional and chromogenic substrates for the identification of medically important yeasts. the ability of the rapid yeast plus system to accurately identify 304 clinical yeast isolates within 5 h was evaluated. the rapid yeast plus method correctly identified 286 (94.1%) of strains to the species level without the need for additional tests. a further 12 strains (3.9%) were classif ...19968727877
meningitis caused by rhodotorula rubra in an hiv-infected patient.meningitis caused by rhodotorula spp. in hiv-infected patients has not been reported previously. we present a brief review of the literature regarding medically important species of rhodotorula and a case of meningitis caused by rhodotorula rubra in an hiv-infected patient. the diagnosis was confirmed by the isolation of the fungus from the csf. the course of rhodotorula-induced meningitis was characterized by gradual development over several months, severe headache and high body temperature. no ...19968912171
asymmetric bioreduction of a ketosulfone to the corresponding trans-hydroxysulfone by the yeast rhodotorula rubra my 2169.a microbial screen identified the yeast rhodotorula rubra my 2169 as a suitable biocatalyst for the asymmetric bioreduction of a ketosulfone (5,6 dihydro-6(s)-propyl-4h-thieno[2,3b] thiopyran-4-one-7,7-dioxide) to the corresponding trans-hydroxysulfone. this synthesizer is a precursor to the carbonic anhydrase inhibitor l-685,393, a new drug candidate targeted for the treatment of ocular glaucoma. process development studies revealed that the rate of bioreduction was sensitive to temperature, ph ...19968987484
detection of extracellular phospholipase activity in candida albicans and rhodotorula rubra.phospholipases are important pathogenicity determinants in candida albicans. they play a significant role in damaging cell membranes and invading host cells. high phospholipase production is correlated with an increased ability of adherence and a higher mortality rate in animal models. by means of an egg yolk-containing agar and the pz (= phospholipase activity zone) value according to price, the present study investigated phospholipase production in 170 strains of c. albicans. at an incubation ...19969066156
growth of saccharomyces cerevisiae, rhodotorula rubra and bullera alba in the presence of beechwood prehydrolyzate-based lignin fractions.the growth of yeast strains saccharomyces cerevisiae, rhodotorula rubra and bullera alba isolated from natural lake microflora was examined in the presence of prehydrolysis lignin and/or its water-soluble derivative. the stimulation effect of the water-soluble lignin derivative was higher in comparison with that of unmodified lignin. the structural changes of the lignin macromolecule by the yeasts determined by ir spectroscopy indicate oxidative degradation and demeth(ox)ylation similar to that ...19969131798
[microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens].microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ...19969190236
[the growth of rhodotorula rubra yeasts and their synthesis of ergosterol on media with lovastatin].the effect of lovastatin, a competing inhibitor of 3-hydroxy-3-methylglutaryl-coa-reductase (hmg-coa-reductase) of the fungal origin on the growth of and ergosterol biosynthesis by rh. rubra vkpm y 1337 was studied. it was shown that the yeast growth was inhibited by lovastatin in concentrations of 0.25 to 5.0 micrograms/ml when the inhibitor was added to the cultivation medium either with the inoculum or at later periods of the yeast cultivation. in concentrations of 2.0 to 5.0 micrograms/ml lo ...19969214284
[scanning electron microscope sudy of the morphology of fungi isolated from patients at the aristide la dantec hospital in dakar, senegal].in order to improve the identification of fungi usually isolated among some patients at dantec hospital, a study by scanning electron microscopy has been carried out. it deals with four species of yeasts (candida albicans, candida parapsilosis, rhodotorula rubra sacharomyces cerevisiae) six species of dermatophytes (trichophyton rubrum, trichophyton soudanense, trichophyton interdigitale, microsporum canis, trichophyton violaceum, microsporum audouinii); two species of mildew, (aspergullus flavu ...19969827085
[phagocytosis of fungi in the peripheral blood neutrophils of two children with cancer during treatment with fluconazole].we report on two children with malignancy who showed fungemia despite the antifungal treatment with fluconazole. case 1 was a 7-year-old girl with a recurrence of stage iv neuroblastoma. she had profound neutropenia and fungemia developed after a month-long treatment with fluconazole. her peripheral blood smear showed phagocytosis in the neutrophils and they were identified as fungi by immunofluorescence method (fungi flora y). she died two days after the diagnosis of fungemia. rhodotorula rubra ...19979311273
moisture and mould problems in schools and respiratory manifestations in schoolchildren: clinical and skin test findings.we performed a clinical study in 99 children attending schools with moisture problems and compared the findings with those of 34 children from a reference school. the aim of the study was to evaluate the possible association between respiratory or allergic diseases in the pupils and moisture or mould problems in the school buildings.19979401510
[molecular epidemiology of candida infections in hiv-infected mothers and their offspring].in a prospective study 15 consecutive children and 12 hiv-infected mothers were examined according to their colonization with candida species in the oral cavity and on the hands. from 39 samples a total of 49 candida isolates could be recovered (26 c. albicans, 4 c. glabrata, 9 rhodotorula rubra, 4 c. dubliniensis, 3 c. tropicalis, 2 c. famata, 1 s. cerevisiae). antifungal susceptibility testing revealed fluconazole mics from 0.09 microgram/ml to 100 micrograms/ml. genotyping was done with an ap ...19979471841
fungal microflora biodiversity as a function of pollution in oued sebou (morocco).an inventory of the fungal microflora present in sediments collected at 11 sites along oued sebou has been established. the influence of some climatic parameters (temperature, rainfall) and of pollution on the composition of the fungal communities has been questioned. emericella nidulans, eurotium amstelodami, neosartotya fisheri var. glabrum (ascomycetes), dreschslera biseptata (dematiaceae), penicillium citrinum (mucedinaceae) and phoma sp. (sphaeropsidale) can be considered as strains sensiti ...19979253167
extraction of lithium from spodumene by bioleaching.the recovery of lithium from spodumene (6.9% li2o) by bioleaching was investigated. this process was carried out using heterotrophic micro-organisms previously isolated from the mineral. penicillium purpurogenum, aspergillus niger and rhodotorula rubra were assayed separately. two different media were used for bioleaching; one of them (m2 medium) was highly limited in mg2+, fe2+ and k+. the assays were carried out in 500 ml erlenmeyer flasks with 1 g of ground mineral at 50-80 mesh and 150 ml of ...19979351258
simultaneous enzymatic synthesis of gluconic acid and sorbitol: production, purification, and application of glucose-fructose oxidoreductase and gluconolactonase.with regard to the enzymatic synthesis of sorbitol and gluconic acid, a screening was carried out to identify promising producers of glucose-fructose oxidoreductase (gfor) and gluconolactonase (gl). zymomonas mobilis dsm 473 and rhodotorula rubra dsm 70403 have been selected for the synthesis of gfor and gl, respectively. maximal enzyme production by these organisms has been achieved at d-glucose concentrations of 200 and 150 g/l, respectively. both gfor and gl were purified and characterized wi ...199718576080
in vitro activity of the new triazole voriconazole (uk-109,496) against opportunistic filamentous and dimorphic fungi and common and emerging yeast pathogens.the in vitro antifungal activity of a new triazole derivative, voriconazole, was compared with those of itraconazole and amphotericin b against 67 isolates of aspergillus flavus, aspergillus fumigatus, bipolaris spp., fusarium oxysporum, fusarium solani, pseudallescheria boydii, rhizopus arrhizus, blastomyces dermatitidis, histoplasma capsulatum, and sporothrix schenckii. the in vitro activities of voriconazole were also compared with those of amphotericin b, fluconazole, and itraconazole agains ...19989431946
the diagnostic usefulness of bone marrow cultures in patients with fever of unknown origin.bone marrow cultures (bmcs) and blood cultures (bcs) are frequently obtained in the evaluation of fever of unknown origin (fuo). however, the low yield of clinically significant isolates leads to questions about their cost-effectiveness. we retrospectively compared bmc with bc and studied the usefulness of bone marrow trephine biopsy (bmtb) histopathology in detecting infection in an unselected population of 61 patients with fuo, among whom 215 bmcs had been performed. for patients who had under ...19989704612
biocidal activity in plant pathogenic acidovorax, burkholderia, herbaspirillum, ralstonia and xanthomonas spp.antibacterial and antifungal activity was investigated for strains of acidovorax spp., burkholderia spp., herbaspirillum rubrisubalbicans and ralstonia solanacearum; strains representing 118 species and pathovars of xanthomonas were also tested for phytotoxic capacity. antibacterial activity was present in all burkholderia spp. except b. andropogonis, in biovars ii and iii of r. solanacearum but not in biovars i and iv, and in two strains of xanthomonas. little antibacterial activity was recorde ...19989633641
space flight micro-fungi after 27 years storage in water and in continuous culture.four species of micro-fungi were selected for study in the national aeronautics and space administration (nasa) apollo microbial ecology evaluation device (meed) mycology experiments. trichophyton terrestre, rhodotorula rubra, saccharomyces cerevisiae and chaetomium globosum were selected from a series of preflight test fungi for the meed mycology studies during the 2 years prior to the actual flight (volz, 1971a, 1972b). conidia of t. terrestre, ascospores of c. globosum and yeast cells of r. r ...199810093232
ige, iga, and igg responses to common yeasts in atopic patients.this study was undertaken to analyze the differences in exposure and sensitization to five common environmental yeasts. the responses of igg, iga, and ige to candida albicans, c. utilis, cryptococcus albidus, rhodotorula rubra, and saccharomyces cerevisiae and purified s. cerevisiae enolase were analyzed by immunoblotting (ige-ib), and the cross-reactivity of their ige-binding components by ige-ib inhibition. twenty atopic subjects, with asthma, allergic rhinitis, or atopic dermatitis were inclu ...19989636810
rapid identification of candida dubliniensis by indirect immunofluorescence based on differential localization of antigens on c. dubliniensis blastospores and candida albicans germ tubes.there is a clear need for the development of a rapid and reliable test for the identification of candida dubliniensis and for the discrimination of this species from candida albicans. in the present study we have investigated the potential use of c. dubliniensis-specific antigens as a basis for its identification. we produced an anti-c. dubliniensis serum which, after adsorption with c. albicans blastospores, was found to differentially label c. dubliniensis isolates in an indirect immunofluores ...19989705368
pseudohyphal variations of yeasts exposed to specific space flight parameters.phenotypes of saccharomyces cerevisiae and rhodotorula rubra exposed to specific parameters of space flight, which were measured both quantitatively and qualitatively, produced variations in pseudohyphal formation. both the length of the parent and branch psuedohyphal filaments varied according to specific wavelengths and energy levels of uv light exposures when phenotypic isolates were compared with the parent or ground control isolate of each yeast species.19989881461
prospective study of nosocomial fungal meningitis in children--report of 10 cases.within an 8-year period, 10 cases of fungal nosocomial meningitis in children 0-13 y old were prospectively identified, 3 caused by yeasts other than candida spp. (rhodotorula rubra, aureobasidium mansoni, clavispora lusitaniae) and 7 by candida albicans. seven patients survived. whereas 3 neonates with fungal meningitis (all due to c. albicans) died. risk factors for fungal nosocomial meningitis included cancer (2 children), previous neurosurgery (2 children), cranial trauma (1 case) and premat ...199810066050
yeast communities associated with sugarcane in campos, rio de janeiro, brazil.yeast communities associated with sugarcane leaves, stems and rhizosphere during different phases of plant development were studied near campos, in rio de janeiro, brazil. atmospheric temperature, soil granulometry and ph, and sugar cane juice degree brix and ph were determined. yeast communities associated with sugarcane were obtained after cellular extraction by shaking, blending and shaking plus sonication, and cultured on yeast nitrogen base agar plus glucose (0.5%) and yeast extract-malt ex ...199810943361
amphotericin b lipid complex therapy in an aids patient with rhodotorula rubra fungemia. 19989798050
essential interactions between thiobacillus ferrooxidans and heterotrophic microorganisms during a wastewater sludge bioleaching process.the stimulating effect of heterotrophic microorganisms was investigated on the growth and on the ferrous iron oxidation of thiobacillus ferrooxidans in synthetic media and in wastewater sludge. the addition of a sediment. rhodotorula rubra isolate or a strain of t. acidophilus on two-layer agarose-gelled medium doubled the plating efficiency of t. ferrooxidans. in liquid cultures, r. rubra had a slight but significant effect on the growth rate of t. ferrooxidans. moreover, the yeast allowed a fa ...199815093092
rhodotorula rubra fungemia in an immunocompromised patient. 199917277475
[activity of exoglycans as sorbents of heavy metal ions].the ability of yeast exoglycans (from cryptococcus laurentii, cr. luteolus, and sporobolomyces albo-rubescens) to absorb copper and lead ions has been studied. the sorption isotherms have been obtained, which indicates that the mechanism of interactions in the system polysaccharide-metal is complex. for the majority of the glycans, the absorption selectivity was considerably higher in the case of copper, as compared to lead. the observed discrepancy in the effects of ph and temperature on the ab ...199910368850
comparative study of the in vitro antifungal activity of bifonazole, naftifine and sertaconazole against yeasts.the in vitro activity of three antifungal agents was tested and compared against 151 yeast strains, including ten candida species, cryptococcus neoformans, rhodotorula rubra, and trichosporon cutaneum. minimum inhibitory concentrations (mics) were determined by a microdilution technique in shadomy modified liquid medium. the mean mics of sertaconazole (0.34 mg/l) were lower than those of naftifine (16.3 mg/l) and bifonazole (13.2 mg/l). these results suggest that sertaconazole is more active aga ...199910435679
in-vitro antifungal activity of liposomal nystatin in comparison with nystatin, amphotericin b cholesteryl sulphate, liposomal amphotericin b, amphotericin b lipid complex, amphotericin b desoxycholate, fluconazole and itraconazole.the in-vitro susceptibilities of 120 clinical isolates of yeasts to liposomal nystatin were compared with those to amphotericin b lipid complex (ablc), liposomal amphotericin b (lab), amphotericin b cholesteryl sulphate (abcd), amphotericin b desoxycholate, nystatin, fluconazole and itraconazole. yeast isolates examined included strains of candida albicans, candida parapsilosis, candida glabrata, candida krusei, candida guilliermondii, candida tropicalis, candida kefyr, candida viswanathii, cand ...199910511410
genotypic relatedness of yeast isolates from women infected with human immunodeficiency virus and their children.the objective of this study was to compare polymerase chain reaction (pcr) fingerprinting with other molecular typing methods as an epidemiologic tool to investigate the transmission of candida strains between hiv-positive mothers and their children. forty-nine yeast strains (including candida albicans, candida glabrata, rhodotorula rubra, candida tropicalis, candida famata, candida dubliniensis, saccharomyces cerevisiae) from 30 individuals (15 children and 15 hiv-infected mothers or accompanyi ...199910536430
cross-reacting ige and igg antibodies to pityrosporum ovale mannan and other yeasts in atopic dermatitis.atopic dermatitis (ad) patients often demonstrate positive skin prick test results and serum ige antibodies to a range of different yeasts. this has been thought to be due to cross-reactivity. in this study, the cross-reactivity of ige and igg antibodies between mannan and crude antigens of pityrosporum ovale, candida albicans, and saccharomyces cerevisiae and crude antigens of cryptococcus albidus and rhodotorula rubra was examined by rast and elisa inhibition with two serum pools of ad patient ...199910536885
[determination of the in vitro antifungal susceptibility of clinically important yeasts using the sensititre system].using sensititre (accumed, usa) we studied the in vitro antifungal activity of amphotericin b, fluconazole, itraconazole, ketoconazole and 5-fluorocytosine against 250 clinical yeast isolates taken from different hospitals, including candida (151 c. albicans, 15 c. krusei, 14 c. parapsilosis, 11 c. tropicalis, 10 c. glabrata, 4 c. guilliermondii, 3 c. rugosa, 2 c. viswanathii, 2 c. famata and 2 c. kefyr), cryptococcus (32 c. neoformans and 1 c. laurentii), trichosporon (2 isolates) and rhodotoru ...199910562673
carboxylic acid microbial metabolites of the natural benzoquinone, maesaninmaesanin (1) is a naturally occurring bioactive benzoquinone isolated from the fruits of maesa lanceolata (myrsinaceae). three carboxylic acid metabolites of maesanin were isolated in the course of a prospective microbial transformation study. the first metabolite, 2, was produced by lipomyces lipofer atcc 10742 and was characterized as (z)-15-(2'-hydroxy-5'-methoxy-3', 6'-dioxocyclohexa-1',4'-dienyl)pentadec-5-enoic acid. metabolites 3 and 4 were produced by rhodotorula rubra atcc 20129 and wer ...199910346952
antimicrobial activity of wedelia trilobata crude extracts.a biological screening of activity against gram-positive and gram-negative bacteria, yeasts, and fungi of crude extracts from wedelia trilobata is reported. the n-hexane extract showed antibacterial activity against bacillus subtilis, mycobacterium smegmatis, staphylococcus aureus, and staphylococcus epidermidis (gram-positive bacteria); along with proteus vulgaris, pseudomonas aeruginosa, salmonella group c, salmonella paratyphi, and shigella sonnei (gram-negative bacteria). the ethyl acetate e ...199910374253
rhodotorula rubra fungaemia in an immunosuppressed patient.we report the case of a patient who, following surgical removal of an extensive tumour of the bowel, developed fungaemia. the yeast was isolated from repeated blood and urine cultures and identified as rhodotorula rubra on the basis of macroscopic and microscopic features. following treatment with amphotericin b, the patient's condition improved and the cultures became sterile.199910343949
invasive yeast infections other than candida spp. in acute leukaemia.during the last two decades, the treatment of leukaemia has changed significantly; increasing intensity of chemotherapy and bone marrow transplantation have lead to profound immunosuppression, prolonged stays in hospital, vascular catheterization, administration of broad spectrum antimicrobial agents and extensive use of prophylactic antifungal drugs. all but the last of these risk factors have increased the incidence of fungal infections in leukaemic patients and have significantly changed the ...199910204120
biological and molecular detection of toxic lipodepsipeptide-producing pseudomonas syringae strains and pcr identification in plantstoxin-based identification procedures are useful for differentiating pseudomonas syringae pathovars. a biological test on peptone-glucose-nacl agar in which the yeast rhodotorula pilimanae was used proved to be more reliable for detecting lipodepsipeptide-producing strains of p. syringae than the more usual test on potato dextrose agar in which geotrichum candidum is used. a pcr test performed with primers designed to amplify a 1, 040-bp fragment in the coding sequence of the syrd gene, which wa ...199910223977
in vitro activities of the new antifungal triazole sch 56592 against common and emerging yeast pathogens.a broth microdilution method performed in accordance with the national committee for clinical laboratory standards guidelines was used to compare the in vitro activity of the new antifungal triazole sch 56592 (sch) to that of fluconazole (flc), itraconazole (itc), and ketoconazole (keto) against 257 clinical yeast isolates. they included 220 isolates belonging to 12 different species of candida, 15 isolates each of cryptococcus neoformans and saccharomyces cerevisiae, and seven isolates of rhodo ...200010602757
the oxidative stress-sensitive yap1 null strain of saccharomyces cerevisiae becomes resistant due to increased carotenoid levels upon the introduction of the chlamydomonas reinhardtii cdna, coding for the 60s ribosomal protein l10a.the saccharomyces cerevisiae yap1 null strain was transformed with a chlamydomonas reinhardtii cdna expression library. a 688-bp cdna fragment, coding for the 60s ribosomal protein l10a (rpl10a), restored the capacity of the s. cerevisiae yap1 null strain to resist oxidative stress. the rpl10a gene is a single-copy gene in c. reinhardtii and encodes a constitutively produced 1.35-kb mrna. the deduced 214-residue amino acid sequence was highly related with rpl10a proteins from eukarya (between 46 ...200010673398
[evaluation of a new method for antifungal drugs susceptibility testing to yeasts].we compared the etest with a broth microdilution method (fp panel), performed according to the national committee for modified clinical laboratory standards (nccls) document m27-p guidelines, for determining the mics of 81 clinical isolates of yeasts (7 candida albicans, 8 candida glabrata, 10 candida parapsilosis, 6 pichia anomala, 10 candida tropicalis, 4 candida guilliermondii, 4 candida krusei, 6 trichosporon cutaneum, 5 candida ciferrii, 3 candida famata, 4 candida norvegensis, 2 rhodotorul ...200010783576
a novel ortho-dehalogenation reaction of 2-chlorocinnamic acid catalyzed by the pink yeast rhodotorula rubra y-1529.in the present study, a resting cells suspension of rhodotorula rubra y-1529 was shown to have the capacity to perform an ortho-dehalogenation reaction on 2-chlorocinnamic acid. the results from the biodegradation of u-[14c]benzoic acid, cinnamic acid, 3-chlorocinnamic acid and 4-chlorocinnamic acid suggest that the first step of the ortho-dehalogenation reaction occurred during the oxidation of the unsaturated c3 side chain of 2-chlorocinnamic acid to 2-chlorobenzoic acid. none of the 2-chlorob ...200010789983
screening for antimycotic properties of 56 traditional chinese drugs.in the present study we screened extracts of 56 widely used dried chinese medical plants or their parts (tcd) for their antimycotic properties against pathological phyla of aspergillus fumigatus, candida albicans, geotrichum candidum and rhodotorula rubra. the highest activity against aspergillus fumigatus was shown by carthamus tinctorius l. (flos) and rheum palmatum l. (radix et rhizoma) and against candida albicans, scutellaria baicalensis georgi (radix) had the highest activity. the highest ...200010815018
[the effect of yeast glycans in experimental lps-induced endotoxicosis].the immunomodulating action of yeast glycans obtained from rhodotorula rubra (rhonasan) and sporobolomyces albo-rubescens (heteroglycan sp-50) was studied under the conditions of experimental endotoxicosis in mice, induced by the injection of the sublethal dose of escherichia coli lipopolysaccharide (lps). in endotoxicosis glycans produced a protective effect, depending on the time of injection. the study demonstrated that after the injection of glycans the survival rate of the animals increased ...200010876900
case report. rhodotorula rubra fungaemia due to use of indwelling venous catheters.rhodotorula has been an infrequent cause of infection in humans but there have been some case reports about this systemic yeast infection. in this article, a rhodotorula rubra fungaemia due to an indwelling catheter in a 23-year-old woman who had been diagnosed with non-hodgkin's lymphoma grade iv b is described.200010948821
lactoferrin is responsible for the fungistatic effect of human milk.human milk has recognized anti-microbial effects and it has been repeatedly shown that breast-fed infants have fewer and less severe infections than formula-fed infants. while most studies have focused on anti-bacterial and anti-viral activities few have focused on the anti-fungal effect of human milk. dermal and other infections caused by fungi are common in very low birth weight (vlbw) infants. using a liquid culturing method and candida albicans and rhodotorula rubra as representative fungi, ...200010996747
bioactivity studies of extracts from tridax procumbens.an updated review on the biological activity of tridax procumbens is presented. a detailed biological screening comprised of gram-positive and gram-negative bacteria, yeasts and fungi using crude extracts of this plant was undertaken. the n-hexane extract of the flowers showed activity against escherichia coli. the same extract of the whole aerial parts was active against mycobacterium smegmatis, escherichia coli, salmonella group c and salmonella paratyphi. the ethyl-acetate extract of the flow ...200011185735
molecular phylogenetics of the genus rhodotorula and related basidiomycetous yeasts inferred from the mitochondrial cytochrome b gene.phylogenetic relationships of basidiomycetous yeasts, especially of the genus rhodotorula, were studied using partial sequences of the mitochondrial cytochrome b gene. the results demonstrated that the basidiomycetous yeasts under investigation distributed into two main clusters: one containing tremellales, filobasidiales and their anamorphs and the other containing ustilaginales, sporidiales and their anamorphs. this clustering in turn correlates with cell wall biochemistry, presence or absence ...200111411687
successful treatment of capd peritonitis caused by rhodotorula mucilaginosa. 200111783780
rhodotorula rubra infection in a kidney transplant patient with pancytopenia. 200111146587
prevalence of yeasts and mycelial fungi in bovine parasitic otitis in the state of minas gerais, brazil.infestations by rhabditiform nematodes and acarids of the genus raillietia are considered the primary causes of external otitis in cattle in tropical regions. recently, yeasts of the genus malassezia have been associated with a relatively high percentage of otitis cases, but the occurrence of other yeasts and mycelial fungi has not yet been reported in the literature. this work studied the presence of fungi in the ear canal of 45 cattle with external parasitic otitis. the results were positive f ...200111708682
structural and functional analysis of horse cathelicidin peptides.cathelicidin-derived antimicrobial peptides are a component of the peptide-based host defense of neutrophils and epithelia, with a widespread distribution in mammals. we recently reported the cdna sequences of three putative horse myeloid cathelicidins, named ecath-1, -2, and -3. a western analysis was performed to investigate their presence in neutrophils and processing to mature peptides. ecath-2 and ecath-3, but not ecath-1, were found to be present in uncleaved forms in horse neutrophils. th ...200111181349
lipid composition of some yeast strains from livingston island, antarctica.yeast strains cryptococcus albidus, cryptococcus laurentii, rhodotorula minuta were isolated from a moss sample. candida oleophila and rhodotorula mucilaginosa were isolated from a soil sample taken from livingston island. antarctica. fatty acid, phospholipid, sterol and tocopherol composition was determined in separated lipid fraction after fermentation in a medium containing glucose, peptone and yeast extract. unsaturated fatty acids, mainly oleic (51-65%) and linoleic (9.5-16.8%), predominate ...200111899472
decaying wood in tree trunk hollows as a natural substrate for cryptococcus neoformans and other yeast-like fungi of clinical interest.the occurrence of cryptococcus neoformans var. neoformans and other yeast-like fungi of clinical interest in decaying wood inside tree trunk hollows, bark and other plant materials is reported. the var. neoformans was isolated from 3 of 45 (6.6%) wood and one of 390 eucalyptus bark samples. two of the positive wood samples came from a tree trunk hollow of butea monosperma (family: papilionaceae) growing in roshan ara garden, old delhi whereas the third was from a trunk hollow of tamarindus indic ...200111554580
a survey of yeasts in traditional sausages of southern italy.the evolution of the yeast population during manufacturing and ripening of 'salsiccia sotto sugna', a typical salami of the lucania region (southern italy), was investigated. four different batches, produced in four farms in lucania, were studied. each batch showed a specific yeast population, and the most frequently isolated yeasts belonged to debaryomyces hansenii and its anamorph candida famata, and rhodotorula mucilaginosa. yarrowia lipolytica was isolated from three sausage batches. the y. ...200112702361
a new isolation method for labyrinthulids using a bacterium, psychrobacter phenylpyruvicus.a new isolation method for labyrinthulids, marine microbes with spindle-shaped vegetative cells and gliding movement, is presented. the method for isolating labyrinthulids has been found to be more difficult and less reproducible than that for thraustochytrids, classified in the same order. so far serum seawater agar fortified with antibiotics has been proposed to be the best for isolation of labyrinthulids. the method presented here involves placing plant samples on an agar medium on which a ma ...200114961392
[some aspects of fungi multifocal invasions connected with the genital organs in women].the aim of presented study was analysis of fungal invasions of the genital organs, oral cavity and digestive tract in 799 women. fungal strains were identified using api 20c and api 20c aux (biomérieux); they were as follows: candida albicans, c. krusei, c. tropicalis, c. guilliermondii, c. kefyr, c. glabrata, c. famata, c. parapsilosis, rhodotorula rubra, geotrichum candidum. the number of fungal strains isolated from the oral cavity ontocenosis was the highest (41.8 +/- 2.4%). fungal strains i ...200116897965
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