Publications

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[ultrastructure of penicillium vitale pidopl. et bilai--producer of catalase and glucose oxidase]. 2006604723
[comparative evaluation of the enzymatic activity of the mycelial homogenates from penicillium vitale pidopl. et bilai broken down by different methods]. 2006994870
[study of the amino acid makeup of the proteins in the mycelial pellicle of penicillium vitale pid. et bil]. 20064840331
[comparative characteristics of catalase from the fungus penicillium vitale, which is synthesized under different nutritional conditions].a comparative study of properties (absorption spectra, thermostability, ph optimum, polyacrylamide gel electrophoresis, deae-cellulose separation) and structure (amino acid composition, finger-prints, carbohydrate composition) was performed for p. vitale catalase synthesized under different medium conditions. in all cases the results were similar. the only difference occured in the amount of synthesized proteins. a conclusion is drawn that under different nourishing conditions of the fungus the ...20063004
[formation of beta-fructofuranosidase by penicillium vitale pidopl. et bilai and other species of the genus penicillium lk]. 20061219324
[industrial experiment in obtaining catalase from penicillium vitale pidopl. et bilai]. 200611395
[on the identity of glucose oxidase and microcid from a new type of penicillium vitale]. 200714475320
strain improvement of penicillium janthinellum ncim 1171 for increased cellulase production.the strain of penicillium janthinellum ncim 1171 was subjected to mutation involving treatment of ethyl methyl sulfonate (ems) for 24h followed by uv-irradiation for 3min. successive mutants showed enhanced cellulase production (ems-uv-8), clearance zone on avicel containing plate (sm2) and rapid growth on walseth cellulose agar plates containing 0.2% 2-deoxy-d-glucose (sm3). these mutants were transferred to walseth cellulose plates containing higher concentration (1.5%) of 2-deoxy-d-glucose (s ...200717097876
indole alkaloids produced by a marine fungus isolate of penicillium janthinellum biourge.three new indole alkaloids, shearinines d, e, and f (1-3), together with the known shearinine a (4) were isolated from the marine-derived strain of the fungus penicillium janthinellum biourge. the chemical structures of 1-4 were established by 2d nmr and hreims data. shearinines a, d, and e induce apoptosis in human leukemia hl-60 cells, and shearinine e also inhibits egf-induced malignant transformation of jb6 p+ cl 41 cells in a soft agar.200717555349
effect of germicidal uvc light on fungi isolated from grapes and raisins.to examine how uvc affects the different genera of fungi commonly isolated from grapes, with the aim of understanding changes in mycobiota during grape ripening and possible applications for preventing grape decay during storage.200717718833
studies on the interaction between grape-associated filamentous fungi on a synthetic medium.eleven fungi isolated from grapes and sun-dried grapes (alternaria alternata, cladosporium herbarum, eurotium amstelodami, penicillium janthinellum, p. decumbens, trichoderma harzianum, candida sp., aspergillus carbonarius ota-negative, a. carbonarius ota-positive, a. niger var. niger. and a. japonicus var. aculeatus), were grown in snm medium at different water activities (0.82-0.97) and temperature (20-40 degrees c) levels for 18 days. pairs of one aspergillus faced with one non-aspergillus we ...200716996158
effect of intra and interspecific interaction on ota production by a. section nigri in grapes during dehydration.the aim of this study was to assess the colonizing capability and ota production of different populations of aspergillus section nigri spp. in grapes, as affected by the interactions with other fungi, during a simulated in vitro sun-drying. mature white grapes were divided into two lots of healthy and artificially injured grapes and inoculated with a. section nigri spp. (a. carbonarius ota producer, a. niger aggregate ota producer, a. niger aggregate ota non-producer), eurotium amstelodami and p ...200717188203
the structures and electronic configuration of compound i intermediates of helicobacter pylori and penicillium vitale catalases determined by x-ray crystallography and qm/mm density functional theory calculations.the structures of helicobacter pylori (hpc) and penicillium vitale (pvc) catalases, each with two subunits in the crystal asymmetric unit, oxidized with peroxoacetic acid are reported at 1.8 and 1.7 a resolution, respectively. despite the similar oxidation conditions employed, the iron-oxygen coordination length is 1.72 a for pvc, close to what is expected for a fe=o double bond, and 1.80 and 1.85 a for hpc, suggestive of a fe-o single bond. the structure and electronic configuration of the oxof ...200717358056
effect of preharvest fungicides and interacting fungi on aspergillus carbonarius growth and ochratoxin a synthesis in dehydrating grapes.to evaluate the effect of preharvest grape pesticides in aspergillus section nigri infection in dehydrating grapes and the final ochratoxin a (ota) content. additionally, the effect of coinoculation of moulds frequently isolated from grapes and raisins on aspergillus section nigri infection was studied.200717651218
[mechanism of labilization of penicillium vitale glucose oxidase].the article deals with conditions for splitting the penicillium vitale glucosooxidase molecule into apoenzyme and coenzyme, for reconstruction of the enzyme from apoenzyme and fad as well as for the effect of some factors ("labilizing" fraction, dithiotreitol) on it. specific activity of the reconstructed enzyme is on the average 85% of the initial enzyme specific activity: "the labilizing" fraction inhibits reconstruction of glucosooxidase from apoenzyme and fad, that is due to oxidation of the ...2008867542
[natural variability of penicillium vitale pidopl. et bilai and characteristics of active variants of the fungus that produce glucose oxidase]. 2008916914
[cu and fe bioleaching in low-grade chalcopyrite and bioleaching mechanisms using penicillium janthinellum strain gxcr].bioleaching of cu and fe in low-grade chalcopyrite using penicillium janthinellum strian gxcr was studied. as a result, shaking bioleaching was more efficient than submerged bioleaching; cu bioleaching was much better than fe bioleaching; under conditions of optimum carbon source (10% sucrose, w/v), optimum nitrogen source (1.5% nano3, w/v), shaking bioleaching and the optimum combination of conditions (initial ph 6.0 in leaching media, 5% (w/v) 200-mesh ore and initial inocula of 3.0x10(5) coni ...200819256351
[variability of penicillium janthinellum biourge--producer of the antibiotic janthinellin and study of the nuclei during ontogenesis of the fungus]. 20084453214
[effects of pre-treatment on cu2+ absorption of penicillium janthinellum strain gxcr].in order to effectively increase capacity of cu2+ absorption by penicillium from cu2+-containing aqueous solution and to study the mechanisms of absorption, effects of eight pre-treatment methods on cu2+ absorption of penicillium janthinellum strain gxcr were compared. the results showed that the efficiency of cu2+ absorption obviously increased through pre-treatment by homogenization, homogenization-basification (naoh), oven dry (80 degrees c), homogenization-salinification (nacl), homogenizati ...200919441230
the molecular mechanism of the catalase reaction.catalases are ubiquitous enzymes that prevent cell oxidative damage by degrading hydrogen peroxide to water and oxygen (2h(2)o(2) --> 2 h(2)o + o(2)) with high efficiency. the enzyme is first oxidized to a high-valent iron intermediate, known as compound i (cpd i) which, in contrast to other hydroperoxidases, is reduced back to the resting state by further reacting with h(2)o(2). by means of hybrid qm/mm car-parrinello metadynamics simulations, we have investigated the mechanism of the reduction ...200919653683
a new indoloditerpene derivative, penijanthine a, isolated from penicillium janthinellum.in a screen searching for new bioactive agents, a new indoloditerpene, penijanthine a (1), was isolated from penicillium janthinellum ifm 55557. the structure of 1 was established on the basis of spectroscopic and chemical investigation, as well as detailed comparison with the spectroscopic and physico-chemical data of paxilline (2), which was isolated along with 1.200918850072
culture-independent characterization of bacteria and fungi in a poultry bioaerosol using pyrosequencing: a new approach.work in animal production facilities often results in exposure to organic dusts. previous studies have documented decreases in pulmonary function and lung inflammation among workers exposed to organic dust in the poultry industry. bacteria and fungi have been reported as components of the organic dust produced in poultry facilities. to date, little is known about the diversity and concentration of bacteria and fungi inside poultry buildings. all previous investigations have utilized culture-base ...201021058154
screening filamentous tropical fungi for their nutritional potential as sources of crude proteins, lipids and minerals.the use of fungal and yeast biomass in foodstuff, either as supplements or as major ingredients in formulations is an area of growing interest for the modern food industry. the aim of this study was to evaluate the nutritional potential of biomasses obtained from filamentous fungi penicillium sclerotiorum, penicillium janthinellum, rhizopus stolonifer and syncephalastrum racemosum. biomasses presented 26-37% of total proteins, 1.7-3.5% of lipids and 4.6-9.1% of ashes. the humidity level reached ...201021339148
functional characterization of the copper-transporting p-type atpase gene of penicillium janthinellum strain gxcr.copper (cu)-transporting p-type atpase (ctpa) genes have been documented to play an important role in resistance to heavy metals. however, our understanding of roles of ctpa genes of the filamentous fungi was based only on sequence similarity prediction before. in a previous study, we isolated a penicillum janthinellum strain gxcr of higher tolerance to cu (200 mm). in this study, we cloned the partial cdna of ctpa gene, named pcpa, from the strain gxcr. sequence alignment indicated that the clo ...201020127468
enzymatically synthesized polyaniline layer for extension of linear detection region of amperometric glucose biosensor.in this article a new method for fabrication of enzymatic electrodes suitable for design of amperometric glucose biosensor and/or anode of biofuel cell powered by glucose is presented. glucose oxidase (gox) e.c. 1.1.3.4. from penicillium vitale was immobilized on the carbon rod electrode by cross-linking it with glutaraldehyde (gox-electrode). catalytic activity of immobilized gox was exploited for polymerisation of aniline by taking a high concentration of hydrogen peroxide produced during the ...201020638831
cloning of exoinulinase gene from penicillium janthinellum strain b01 and its high-level expression in pichia pastoris.the aim of this study is to improve exoinulinase production by expression of a cloned exoinulinase gene inua1 (genbank accession no. jf961344) from penicillium janthinellum strain b01 in pichia pastoris.201121895898
comparative production of cellulases by mutants of penicillium janthinellum ncim 1171 and its application in hydrolysis of avicel and cellulose.mutants of penicillium janthinellum ncim 1171 were evaluated for cellulase production using both submerged fermentation (smf) and solid state fermentation (ssf). mutant eu2d-21 gave highest yields of cellulases in both smf and ssf. hydrolysis of avicel and cellulose were compared using smf and ssf derived enzyme preparations obtained from eu2d-21. surprisingly, the use of ssf derived preparation gave less hydrolysis compared to smf derived enzymes. this may be due to inactivation of β-glucosidas ...201121300541
penicillium araracuarense sp. nov., penicillium elleniae sp. nov., penicillium penarojense sp. nov., penicillium vanderhammenii sp. nov. and penicillium wotroi sp. nov., isolated from leaf litter.several species of the genus penicillium were isolated during a survey of the mycobiota of leaf litter and soil in colombian amazon forest. five species, penicillium penarojense sp. nov. (type strain cbs 113178(t) = ibt 23262(t)), penicillium wotroi sp. nov. (type strain cbs 118171(t) = ibt 23253(t)), penicillium araracuarense sp. nov. (type strain cbs 113149(t) = ibt 23247(t)), penicillium elleniae sp. nov. (type strain cbs 118135(t) = ibt 23229(t)) and penicillium vanderhammenii sp. nov. (type ...201120639229
[Isolation and identification of dominant microorganisms in rhizosphere of continuous cropping with peanut].We isolated and identified dominant microorganisms from the rhizosphere of continuous cropping with peanut, to study the relationship between dominant microorganisms and peanut continuous cropping.201121866710
proton transfer drives protein radical formation in helicobacter pylori catalase but not in penicillium vitale catalase.heme catalases prevent cells from oxidative damage by decomposing hydrogen peroxide into water and molecular oxygen. here we investigate the factors that give rise to an undesirable side reaction competing with normal catalase activity, the migration of a radical from the heme active site to the protein in the principal reaction intermediate compound i (cpd i). recently, it has been proposed that this electron transfer reaction takes place in cpd i of helicobacter pylori catalase (hpc), but not ...201121381757
Arsenic biotransformation by arsenic-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1.Bioremediation of arsenic (As)-contaminated soil using microorganisms has been a focus of research because it is environment friendly and cost-effective. The As-resistant fungi Trichoderma asperellum SM-12F1, Penicillium janthinellum SM-12F4, and Fusarium oxysporum CZ-8F1 were exposed to 50 mg l(-1) of As(V), and the biotransformation of As and the concomitant variance of Eh and pH in the media were studied. Fresh weights of all three isolates increased and then decreased depended on cultivation ...201121920586
thirty years of heme catalases structural biology.about thirty years ago the crystal structures of the heme catalases from penicillium vitale (pvc) and, a few months later, from bovine liver (blc) were published. both enzymes were compact tetrameric molecules with subunits that, despite their size differences and the large phylogenetic separation between the two organisms, presented a striking structural similarity for about 460 residues. the high conservation, confirmed in all the subsequent structures determined, suggested a strong pressure t ...201122209752
[characteristics of the eukaryotic community structure in acid mine drainage lake in anhui province, china].we characterized eukaryotic community structure and the relationship between the community structure and environmental factors in acidic mine drainage (amd) lake of a sulfide mine in anhui province, china.201223115972
arsenate reduction and methylation in the cells of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 investigated with x-ray absorption near edge structure.synchrotron radiation-based x-ray absorption near edge structure (xanes) was introduced to directly analysis chemical species of arsenic (as) in the cells of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 capable of as accumulation and volatilisation. after exposure to as(v) of 500 mg l(-1) for 15 days, a total of 60.5% and 65.3% of the accumulated as in the cells of t. asperellum sm-12f1 and p. janthinellum sm-12f4, respectively, was as(iii), fol ...201223122191
biotransformation of imperatorin by penicillium janthinellum. anti-osteoporosis activities of its metabolites.imperatorin (imp) is a major constituent of many herbal medicines and possesses anti-osteoporosis activity. the present research work aimed to study the biotransformation processes of imp and evaluated the anti-osteoporosis activity of the transformed metabolites. among 18 strains of filamentous fungi screened, penicillium janthinellum as 3.510 exhibited good capability to metabolise imp to the new derivatives. ten transformed products were isolated and purified, and their structures were identi ...201223497884
[biotransformation of cycloartane-type triterpenoid from myrrh].to study the biotransformation of cycloartan-24-ene-1alpha,2alpha, 3beta-triol isolated from myrrh and find new antitumor bioactive cycloartane-type derivatives.201223252274
fungal endophyte penicillium janthinellum lk5 improves growth of aba-deficient tomato under salinity.an endophytic fungus was isolated from the roots of tomato (solanum lycopersicum mill) and identified as penicillium janthinellum lk5. the culture filtrate (cf) of p. janthinellum significantly increased the shoot length of gibberellins (gas) deficient mutant waito-c and normal dongjin-beyo rice seedlings as compared to control. the cf of p. janthinellum contained gas (ga3, ga4, ga7 and ga12). to assess endophyte-growth promoting and stress-tolerance potential, the cf along with the propagules o ...201323842755
production of intracellular β-xylosidase from the submerged fermentation of citrus wastes by penicillium janthinellum mtcc 10889.production of intracellular β-xylosidase was studied in cultures of penicillium janthinellum grown on citrus fruit waste supplemented cultivation media. both dried orange peel and sweet lime peel could induce the production of this enzyme. the working strain showed a pronounced optimum ph and temperature for β-xylosidase production at 6.0 and 27 °c, respectively. the enzyme production was found to remain stable for a long period of 120 h. orange peel and sweet lime peel showed different response ...201328324373
[stabilizing effect of calcium ions on glucose oxidase of penicillium vitale].reconstruction of p. vitale glucose oxidase from apeonzyme and coenzyme (fad) was studied as affected by iodine acetate as well as mercury and calcium ions. mercury ions, iodine acetate as well as the labilizing fraction (flavin adenine-containing component) are established to inhibit the reconstruction affecting the sulphydryl groups of the apoenzyme which take part in addition of fad to it. calcium ions prevent the effect of the "labilizing" fraction, iodine acetate and mercury ions on the glu ...2013888220
[dissociation of penicillium vitale catalase under the effect of urea and acid ph].conditions are studied for penicillium vitale catalase dissociation into subunits under the effect of urea (0.7-8.5 m) and acid ph (5.0-2.0). in 8.0 m urea (ph 5.0) a molecule of the p. vitale catalase dissociates with formation of the components, the sedimentation coefficient of which is 2.4+/-0.2s, the molecular weight is 153000+/-2800. dissociation at ph 2.2 in 4.0 m urea results in formation of components with the sedimentation coefficient 2.2+/-0.3 s. the catalase molecule dissociation unde ...201318833
[taxonomic position and nitrogen-containing secondary metabolites of the fungus penicillium vitale pidoplichko et bilai apud bilai].the type strain penicillium vitale pidoplichko et bilai apud bilai 1961 vkm f-3624 was found to considerably differ from a sibling species p. janthinellium (syn. p. simplicissimum) in some physiological and morphological features (growth rates at different temperatures, the size of philiades, and the shape of conidia), as well as in the pattern of the nitrogen-containing secondary metabolites produced (roquefortine, 3,12-dihydroroquefortine, meleagrin, aurantioclavine, indole-3-acetic acid, and ...201310920814
cytotoxic metabolites from the cultures of endophytic fungi from panax ginseng.two strains of endophytic fungi, penicillium melinii yuan-25 and penicillium janthinellum yuan-27, with strong anti-pyricularia oryzae activity, were obtained from the roots of panax ginseng. based on bioactivity-oriented isolation, a new benzaldehyde derivative, ginsenocin (1), together with six known compounds, methyl 2,4-dihydroxy-3,5,6-trimethylbenzoate (2), 3,4,5-trimethyl-1,2-benzenediol (3), penicillic acid (4), mannitol (5), ergosterol (6), and ergosterol peroxide (7), were separated fro ...201323793342
antifungal activity and cytotoxicity of isolated compounds from leaves of breonadia salicina.breonadia salicina is used traditionally to treat wounds, ulcers, fevers, headaches, and fungal infections. the aim of this study was to investigate the antifungal activity of the plant extract and compounds isolated there from.201323727185
structure, recombinant expression and mutagenesis studies of the catalase with oxidase activity from scytalidium thermophilum.scytalidium thermophilum produces a catalase with phenol oxidase activity (catpo) that catalyses the decomposition of hydrogen peroxide into oxygen and water and also oxidizes various phenolic compounds. a codon-optimized catpo gene was cloned and expressed in escherichia coli. the crystal structures of native and recombinant s. thermophilum catpo and two variants, h82n and v123f, were determined at resolutions of 2.7, 1.4, 1.5 and 1.9 å, respectively. the structure of catpo reveals a homotetram ...201323519415
isolation, identification and antimicrobial activity of propolis-associated fungi.propolis is a natural product widely known for its medicinal properties. in this work, fungi present on propolis samples were isolated, identified and tested for the production of antimicrobial metabolites. twenty-two fungal isolates were obtained, some of which were identified as alternaria alternata, aspergillus flavus, bipolaris hawaiiensis, fusarium merismoides, lasiodiplodia theobromae, penicillium citrinum, penicillium crustosum, penicillium janthinellum, penicillium purpurogenum, pestalot ...201323439233
[carbohydrate components of the glucose oxidase from penicillium vitale]. 20134441570
[composition and structure of glucose oxidase from penicillium vitale]. 20135556037
[on the mechanism of the stimulatory effect of calcium carbonate on the glucose oxidase and catalase activities of penicillium vitale]. 20135871568
metabolism of nc11 fatty acid fed to trichoderma koningii and penicillium janthinellum ii: production of intracellular and extracellular lipids.little is known about the fungal metabolism of nc10 and nc11 fatty acids and their conversion into lipids. a mixed batch culture of soil fungi, t. koningii and p. janthinellum, was grown on undecanoic acid (uda), a mixture of uda and potato dextrose broth (uda+pdb), and pdb alone to examine their metabolic conversion during growth. we quantified seven intracellular and extracellular lipid classes using iatroscan thin-layer chromatography with flame ionization detection (tlc-fid). gas chromatogra ...201425310811
metabolism of n-c10:0 and n-c11:0 fatty acids by trichoderma koningii, penicillium janthinellum and their mixed culture: i. biomass and co2 production, and allocation of intracellular lipids.the capacity of two soil fungi, trichoderma koningii and penicillium janthinellum, to oxidize n-c10:0 and n-c11:0 fatty acids to co2 and store intracellular lipids during growth is unknown. this article reports for the first time the metabolism of decanoic acid (da, c10:0), undecanoic acid (uda, n-c11:0), a mixture of the acids (uda+da) and a mixture of uda+ potato dextrose broth (pdb) by t. koningii and p. janthinellum and their mixed culture. a control pdb complex substrate was used as a subst ...201425310810
production and purification of a fungal chitosanase and chitooligomers from penicillium janthinellum d4 and discovery of the enzyme activators.chitosanases have received much attention because of their wide range of applications. although most fungal chitosanases use sugar as their major carbon source, in the present work, a chitosanase was induced from a squid pen powder (spp)-containing penicillium janthinellum d4 medium and purified by ammonium sulphate precipitation and combined column chromatography. the purified d4 chitosanase exhibited optimum activity at ph 7-9, 60°c and was stable at ph 7-11, 25-50°c. the d4 chitosanase that w ...201424751281
fungal communities in the garden chamber soils of leaf-cutting ants.leaf-cutting ants modify the properties of the soil adjacent to their nests. here, we examined whether such an ant-altered environment impacts the belowground fungal communities. fungal diversity and community structure of soil from the fungus garden chambers of atta sexdens rubropilosa and atta bisphaerica, two widespread leaf-cutting ants in brazil, were determined and compared with non-nest soils. culture-dependent methods revealed similar species richness but different community compositions ...201423681670
bioethanol production from wheat straw via enzymatic route employing penicillium janthinellum cellulases.this study concerns in-house development of cellulases from a mutant penicillium janthinellum ems-uv-8 and its application in separate hydrolysis and fermentation (shf) and simultaneous saccharification and fermentation (ssf) processes for bioethanol production from pre-treated wheat straw. in a 5l fermentor, the above strain could produce cellulases having activity of 3.1 fpu/ml and a specific activity of 0.83 fpu/mg of protein. in-house developed cellulase worked more efficiently in case of ss ...201425086433
untreated wheat straw: potential source for diverse cellulolytic enzyme secretion by penicillium janthinellum ems-uv-8 mutant.study describes the production of cellulases by penicillium janthinellum ems-uv-8 using untreated wheat straw (ws), treated ws (acid, alkali, steam exploded, organo-solv) and pure cellulosic substrates (avicel, cellulose-ii and carboxymethyl cellulose). severely pretreated ws and cellulose-ii produced more cellulolytic enzymes than untreated samples. xrd and ftir analysis revels that the increase in the amorphous structure of pretreated ws/cellulose increases enzyme production. enzyme samples pr ...201526291411
paths and determinants for penicillium janthinellum to resist low and high copper.copper (cu) tolerance was well understood in fungi yeasts but not in filamentous fungi. filamentous fungi are eukaryotes but unlike eukaryotic fungi yeasts, which are a collection of various fungi that are maybe classified into different taxa but all characterized by growth as filamentous hyphae cells and with a complex morphology. the current knowledge of cu resistance of filamentous fungi is still fragmental and therefore needs to be bridged. in this study, we characterized cu resistance of pe ...201526265593
phytohormones enabled endophytic fungal symbiosis improve aluminum phytoextraction in tolerant solanum lycopersicum: an examples of penicillium janthinellum lk5 and comparison with exogenous ga3.this work investigates the potentials of fungal-endophyte penicillium janthinellum lk5 (pjlk5) and its inherent gibberellic acid (ga3) as reference to enhance aluminum (al) induced toxicity in tolerant tomato (solanum lycopersicum) plants. initial screening showed significantly higher uptake of al by pjlk5. aluminum stress (100 μm) significantly retarted plant growth in control plants. conversely pjlk5 and ga3 application significantly increased morphological attributes of al-tolerant tomato pla ...201525885165
selection and molecular characterization of cellulolytic-xylanolytic fungi from surface soil-biomass mixtures from black belt sites.plant biomass is an abundant renewable natural resource that can be transformed into chemical feedstocks. enzymes used in saccharification of lignocellulosic biomass are a major part of biofuel production costs. a cocktail of cellulolytic and xylanolytic enzymes are required for optimal saccharification of biomass. accordingly, thirty-two fungal pure cultures were obtained from surface soil-biomass mixtures collected from black belt sites in alabama by culturing on lignocellulosic biomass medium ...201525817459
[catalase activity as affected by carnosine and sodium nitrite in vitro].it was shown in vitro that the activity of catalase penicillium vitale decreases when treated by sodium nitrite to the greater extent than when affected by dipeptide carnosine (beta-alanyl-l-histidine). at alternating introduction of carnosine and nano2 that component affected the catalase activity which was the first to be introduced. the entering of the next metabolite into the medium did not change the enzyme activity. it is shown that carnosine binds with a molecule of catalase. carnosine ma ...201516100910
extractive cultivation of xylanase by penicillium janthinellum in a poly(ethylene glycol)/cashew-nut tree gum aqueous two-phase system.cultivation of the fungus penicillium janthinellum for xylanase production was studied in a poly(ethylene glycol)/cashew-nut tree gum aqueous two-phase system, using a two-level fractional factorial design. the parameters studied were initial ph, cultivation time, type of agro-industrial residue (oat husk or corn cob), agitation, temperature, and phase-forming polymers. the xylanase produced during fermentation partitioned into the top phase. the agitation and temperature (negative), cultivation ...201515575727
[the kinetic and catalytic properties of penicillium vitale catalase].the steady-state kinetics of catalytic action of penicillium vitale catalase has been studied. the enzyme reaction conforms to the michaelis-menten equation, which is shown by considering the initial velocity of the enzyme-catalytic reaction with increased concentrations of hydrogen-peroxide and sodium perborate. the parameter km value is rather large (231-259) mm. on the other hand the pen, vitale catalase is one of the more active enzymes and shows kcat values equal to 0.8-3.0 x 10(-6) s-1 and ...20159005665
penicillium janthinellum: a source of efficient and high levels of β-glucosidase.penicillium janthinellum strain isolated from leaf litters of oak trees from montane alpine forests of shivalik hills (india) produced high levels of β-glucosidase both during solid-state fermentation (796 units/gds) and shake flask cultures (65.3 units/ml). the peptide mass fingerprinting of the secretome showed a variety of glycosyl hydrolases. β-glucosidase was purified and characterized to be a gh3 family member that had a molecular weight (m r) of 101 kda and pi of 4.5. β-glucosidase was op ...201525351628
[immobilization of penicillium vitale glucose-oxidase on aminosilochrome and properties of immobilized enzyme].penicillium vitale glucose-oxidase modified by means of the carbohydrate component oxidation is added covalently to aminoorganosylochrome. the activity of the immobilized preparations is 20-38% depending on the protein-carrier ration in immobilization. comparison of some properties of native and immobilized glucose-oxidase showed that the rh optimum of the immobilized glucose-oxidase is slightly widened towards the alkaline regions; the immobilized glucose-oxidase possesses a considerably higher ...201538549
[immobilization of penicillium vitale glucose oxidase by aminoethyl cellulose and properties of immobilized enzyme].penicillium vitale glucose oxidase modified by oxidation of the carbohydrate component is in a covalent combination with aminoethyl cellulose (ae-cellulose). with the optimal enzyme: carrier ratio the manifested activity of glucose oxidase in the preparation is 4.3 +/- 0.8% of the attached enzyme activity. it is established that thermostability, stability to an inactivating effect of a labilizing fraction and ph-stability with ph alkaline values in the immobilized glucose oxidase are higher than ...20157281253
[immobilization of penicillium vitale catalase on aminoethyl cellulose and properties of the obtained preparations].preparations of penicillium vitale catalase immobilized by aminoethyl cellulose (ae-cellulose) are obtained using two methods: by the enzyme covalent cross-linking with the carrier by glutaric aldehyde and by the covalent binding of catalase to the carrier aminogroups through the carbohydrate enzyme component. a dependence is established for the degree of catalase binding and catalase activity of the immobilized enzyme on the enzyme carrier in immobilization ratio. the optimal enzyme-carrier rat ...20157281254
[immobilization of penicillium vitale pidopl. et bilai catalase by inorganic carriers].an efficient method is developed for p. vitale catalase immobilization through the oxidized carbohydrate enzyme component, using silochrome. the method provides the enzyme binding without losing its catalytic capacity in the immobilized preparation. when the enzyme is immobilized by high-dispersed silica containing isocyanate, aldehyde groups or active atoms of chlorine, 8, 15, and 20 mg of the enzyme is bounded per 1 g of the carrier, respectively, its catalytic capacity being completely retain ...20156266097
[study of the subunit structure of catalase from penicillium vitale].a molecule of penicillium vitale catalase is shown to dissociate into subunits with the molecular weight 75-80 kdalton. when hemin is splitted off the molecule also disintegrates into subunits equalling 1/4 of the enzyme molecule. the amino acid composition and fingerprints of the catalase subunits were studied. it is supposed that n-terminal residue of the subunit is blocked.20154035792
[application of ultrafiltration for concentration and purification of penicillium vitale catalase].various factors are studied for their effect on the ultrafiltration of penicillium vitale catalase. it is established that acetate cellulose and polyamide membranes may be used for additional purification and deep concentration of native preliminarily purified enzyme solutions. membranes yam-300 and yam-450 are most preferable. at the temperature of 10 degrees c, pressure 0.2 mpa and 100-fold concentration of the catalase solution the enzyme yield at the ultrafiltration stage is 96-100%.20156636309
[stability of penicillium vitale immobilized catalase in continuous decomposition of hydrogen peroxide].the process of hydrogen peroxide continuous decomposition by the preparation of the fungus penicillium vitale catalase immobilized by aminoorganosilica which were activated by glutaric aldehyde, cyanuric chloride or 2,4-toluylene diisocyanate. catalase with an oxidized carbohydrate component was used as well. such a modified enzyme was directly bound with the surface of aminocontaining silica and alumina. it is shown that in the process of h2o2 decomposition the preparations of immobilized catal ...20157281255
demethylation of arsenic limits its volatilization in fungi.arsenic (as) biomethylation is increasingly being regarded as a promising method to volatize as from the environment; however, the as volatilization efficiency of most microorganisms is low. here, the speciation transformation of dimethylarsinic acid (dma) as an important methylation intermediate in the cells of fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1 were investigated. these fungal strains have been certified to volatilize as from as-loade ...201525951513
arsenic speciation transformation and arsenite influx and efflux across the cell membrane of fungi investigated using hplc-hg-afs and in-situ xanes.microorganisms dominated speciation of arsenic (as) play an important role in the biogeochemical cycling of as. in the study, species transformation of arsenite [as(iii)] and as(iii) influx and efflux across the cell membranes of trichoderma asperellum sm-12f1, penicillium janthinellum sm-12f4, and fusarium oxysporum cz-8f1 cells were studied using a cellular lysis plus chromatographic separation method and further the in-situ x-ray absorption near edge structure (xanes) analysis. the results in ...201525460757
biodegradation of kerosene: study of growth optimization and metabolic fate of p. janthinellum sdx7.penicillum janthinellum sdx7 was isolated from aged petroleum hydrocarbon-affected soil at the site of anand, gujarat, india, and was tested for different ph, temperature, agitation and concentrations for optimal growth of the isolate that was capable of degrading upto 95%, 63% and 58% of 1%, 3% and 5% kerosene, respectively, after a period of 16 days, at optimal growth conditions of ph 6.0, 30 °c and 180 rpm agitation. the gc/ms chromatograms revealed that then-alkane fractions are easily degra ...201526273254
[the effect of the quality of the sowing material on the enzyme activity and flavinogenesis of penicillium vitale pidopl. et bilai under industrial conditions]. 20155153983
[hydrodynamic properties and molecular weight of penicillium vitale catalase]. 20164823742
antioxidant and antifungal activity of selected medicinal plant extracts against phytopathogenic fungi.medicinal plants are used by many ethnic groups as a source of medicine for the treatment of various ailments in both humans and domestic animals. these plants produce secondary metabolites that have antimicrobial properties, thus screening of medicinal plants provide another alternative for producing chemical fungicides that are relatively non-toxic and cost-effective.201628852739
chemical characterization of fatty acids, alkanes, n-diols and alkyl esters produced by a mixed culture of trichoderma koningii and penicillium janthinellum grown aerobically on undecanoic acid, potatoe dextrose and their mixture.little is known about the mixed fungal synthesis of high-value aliphatics derived from the metabolism of simple and complex carbon substrates. trichoderma koningii and penicillium janthinellum were fed with undecanoic acid (uda), potatoe dextrose broth (pdb), and their mixture. pyrolysis field ionization mass spectrometry (py-fims) together with (1)h and (13)c nuclear magnetic resonance (nmr) characterized chcl3 soluble aliphatics in the fungal cell culture. data from nmr and py-fims analysis we ...201626852878
production, purification and characterization of an exo-polygalacturonase from penicillium janthinellum sw09.a soil isolate, penicillium janthinellum sw09 has been found to produce significant amounts of an extracellular pectinase subsequently characterized as exo-polygalacturonase (exo-pg). by optimizing growth conditions, p. janthinellum sw09 produced high amount of exo-pg (16.54 units/ml). the crude enzyme was purified by gel filtration chromatography and two exo-pg activity peaks (designated as pgi and pgii) were revealed. on sds-page analysis, purified pgii using deae-sepharose ff column, was foun ...201627142550
antimicrobial activity and acetylcholinesterase inhibition by extracts from chromatin modulated fungi.major health challenges as the increasing number of cases of infections by antibiotic multiresistant microorganisms and cases of alzheimer's disease have led to searching new control drugs. the present study aims to verify a new way of obtaining bioactive extracts from filamentous fungi with potential antimicrobial and acetylcholinesterase inhibitory activities, using epigenetic modulation to promote the expression of genes commonly silenced. for such finality, five filamentous fungal species (t ...201728818332
endophytic paecilomyces formosus lhl10 augments glycine max l. adaptation to ni-contamination through affecting endogenous phytohormones and oxidative stress.this study investigated the ni-removal efficiency of phytohormone-producing endophytic fungi penicillium janthinellum, paecilomyces formosus, exophiala sp., and preussia sp. among four different endophytes, p. formosus lhl10 was able to tolerate up to 1 mm ni in contaminated media as compared to copper and cadmium. p. formosus lhl10 was further assessed for its potential to enhance the phytoremediation of glycine max (soybean) in response to dose-dependent increases in soil ni (0.5, 1.0, and 5.0 ...201728611799
isolation of a fungus pencicillium sp. with zinc tolerance and its mechanism of resistance.a zinc (zn)-tolerant fungus, designated bc109-2, was isolated from rhizosphere soil and was identified as penicillium janthinellum bc109-2 based on its sequence analysis. to understand its zn tolerance mechanisms, a series of studies was carried out addressing the subcellular distribution of zn, its chemical forms, and the antioxidant system (superoxide dismutase, catalase, peroxidase, glutathione reductase, glutathione s-transferase, reduced glutathione, oxidized glutathione and malondialdehyde ...201728887685
new brefeldins and penialidins from marine fungus penicillium janthinellum dt-f29.a fermentation of marine fungus penicillium janthinellum dt-f29 on solid rice medium led to the isolation of three new compounds, brefeldin d (1) and penialidins d-e (5-6), along with other five known brefeldins and penialidins. the structures of above compounds were determined on the basis of ms and nmr analysis.201728722499
a proteomic-based investigation of potential copper-responsive biomarkers: proteins, conceptual networks, and metabolic pathways featuring penicillium janthinellum from a heavy metal-polluted ecological niche.filamentous fungi-copper (cu) interactions are very important in the formation of natural ecosystems and the bioremediation of heavy metal pollution. however, important issues at the proteome level remain unclear. we compared six proteomes from cu-resistant wild-type (wt) penicillium janthinellum strain gxcr and a cu-sensitive mutant (ec-6) under 0, 0.5, and 3 mmol/l cu treatments using itraq. a total of 495 known proteins were identified, and the following conclusions were drawn from the result ...201728488414
penicisulfuranols a-f, alkaloids from the mangrove endophytic fungus penicillium janthinellum hdn13-309.six new epipolythiodioxopiperazine (etp) alkaloids, penicisulfuranols a-f (1-6), were isolated from the mangrove endophytic fungus penicillium janthinellum hdn13-309. all structures including absolute configurations were elucidated on the basis of comprehensive spectroscopic data and ecd calculations. they belong to the unusual family of etps containing sulfur atoms on both α- and β-positions of amino acid residues and a rare 1,2-oxazadecaline core moiety. in addition, compounds 1-6 also possess ...201727992183
penicilones a-d, anti-mrsa azaphilones from the marine-derived fungus penicillium janthinellum hk1-6.four new azaphilones, penicilones a-d (1-4), were isolated from the mangrove rhizosphere soil-derived fungus penicillium janthinellum hk1-6. their planar structures and absolute configurations were determined by extensive analysis of nmr spectroscopic data, ecd spectra, the modified mosher's method, and chemical conversions. interestingly, 1 and 2 had the opposite configuration at c-7 compared to the closely related chloro analogues 3 and 4. ester hydrolysis of 2 and 4 afforded their parental az ...201728248508
concurrent methylation and demethylation of arsenic by fungi and their differential expression in the protoplasm proteome.microbial methylation and demethylation are central to arsenic's (as) biogeochemical cycling. here, the transformations of monomethylarsonic acid (mma(v)) (50 mg l(-1)) for 15 days in cells of as-methylating fungi, fusarium oxysporum cz-8f1, penicillium janthinellum sm-12f4, and trichoderma asperellum sm-12f1, were evaluated, and trace concentrations of as(iii) and as(v) were observed in fungal cell extracts. trace amounts of dma(v) were also detected in mma(v) and p. janthinellum sm-12f4 incuba ...201728336093
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