Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| attraction of adult sweet potato weevils,cylas formicarius elegantulus (summers), (coleoptera: curculionidae), to sweet potato leaf and root volatiles. | a dual-choice olfactometer was developed to study the responses of sweet potato weevils,cylas formicarius elegantulus (summers), to volatiles from the sweet potato,ipomoea batatas (l.) lam. both males and females were attracted by volatiles from sweet potato leaves and a methylene chloride leaf extract. females, but not males, responded to volatiles from storage roots and a methylene chloride root extract. leaves and storage roots from four sweet potato cultivars (centennial, jewel, resisto, and ... | 1989 | 24271909 |
| feeding and oviposition preferences of sweet potato weevil,cylas formicarius elegantulus (summers), on storage roots of sweet potato cultivars with differing surface chemistries. | cores from sweet potato [ipomoea batatas (l.) lam.] storage roots (centennial, jewel, resisto, and regal cultivars) were presented to sweet potato weevils [cylas formicarius elegantulus (summers) (coleoptera; curculionidae)] in multiple-choice, limited-choice, and no-choice bioassays. centennial, a susceptible cultivar in field-plot experiments, was preferred for feeding and oviposition by female weevils in choice bioassays, and for ovi-position in no-choice bioassays, compared to three other cu ... | 1989 | 24271892 |
| possible members of the potyvirus group transmitted by mites or whiteflies share epitopes with aphid-transmitted definitive members of the group. | there are at least ten viruses identified in the literature that resemble definitive potyviruses in having flexuous filamentous particles and inducing the formation of "pinwheel" cytoplasmic inclusions in infected cells but that are transmitted by eriophyid mites, whiteflies or soil fungi and not by aphids, the vectors of the definitive potyviruses. the taxonomic status of these viruses is uncertain at present. using a broadly cross-reactive antiserum raised against the dissociated coat protein ... | 1989 | 2473719 |
| correspondence of minor subunits of plant mitochondrial f1atpase to f1f0atpase subunits of other organisms. | in addition to two major alpha- and beta-subunits, the soluble oligomycin-insensitive f1atpase purified from sweet potato root mitochondria contains four different minor subunits of gamma (mr = 35,500), delta (mr = 27,000), delta' (mr = 23,000), and epsilon (mr = 12,000) (iwasaki, y., and asashi, t. (1983) arch. biochem. biophys. 227, 164-173). among these minor subunits, the delta-subunit specifically cross-reacted with an antibody against the delta-subunit of maize mitochondrial f1 which conta ... | 1989 | 2536736 |
| expression and processing of cyanobacterial mn-stabilizing protein in escherichia coli. | the woxa gene of cyanobacterium anacystis nidulans r2, which encodes the precursor of the mn-stabilizing protein involved in photosynthetic water oxidation, was found to be expressed in escherichia coli. the 30-kda expression product was indistinguishable from the authentic mature protein on sds/page. upon fractionation of e. coli cells, the expression product was co-precipitated with the membrane fraction, which is consistent with the water-insoluble nature of the authentic mature protein. anal ... | 1989 | 2689172 |
| partial cleavage of sweet potato feathery mottle virus coat protein subunit by an enzyme in extracts of infected symptomless leaves. | the coat protein of particles of sweet potato feathery mottle potyvirus (spfmv) extracted from ipomoea spp. migrated in sds-page mainly as bands of m(r) 38,000 (38k), 36k, 32k, 30k. trypsin treatment of the particles resulted in the appearance of only one 30k polypeptide. the inclusion of protease inhibitors in the extraction procedure did not alter the heterogeneity of spfmv coat protein. a partially purified fraction of extracts from recovering, symptomless, but not from healthy leaves of i. n ... | 1989 | 2769226 |
| parotid gland enlargement and female reproductive performance in a papua new guinea highland population. | swelling of the parotid salivary glands is commonly observed among males and females of highland papua new guinea. evidence suggests that the condition is related to the highlanders' high starch consumption in the form of sweet potato. in this paper we regard parotid enlargement as an indicator of high energy intake and test its association with two measures of reproductive performance, the number of live births and living offspring, in a sample of 274 women from lufa in the eastern highlands di ... | 1989 | 2802523 |
| structure and characterization of a cdna clone for phenylalanine ammonia-lyase from cut-injured roots of sweet potato. | a cdna clone for phenylalanine ammonia-lyase (pal) induced in wounded sweet potato (ipomoea batatas lam.) root was obtained by immunoscreening a cdna library. the protein produced in escherichia coli cells containing the plasmid ppal02 was indistinguishable from sweet potato pal as judged by ouchterlony double diffusion assays. the m(r) of its subunit was 77,000. the cells converted [(14)c]-l-phenylalanine into [(14)c]-t-cinnamic acid and pal activity was detected in the homogenate of the cells. ... | 1989 | 16666943 |
| tetracycline production with sweet potato residue by solid state fermentation. | for saving energy in antibiotic production and reducing the amount of agricultural wastes, solid state fermentation was used in this study to produce tetracycline with sweet potato residue by streptomyces viridifaciens atcc 11989. it was found that the optimal media for tetracycline production were sweet potato residue 100 g, organic nitrogen (rice bran, wheat bran, or peanut meal) 20 g, (nh(4))(2)so(4) 2.4 g, kh(2)po(4) 0.4 g, caco(3) 1.8 g, nacl 0.6 g, mgcl(2) 0.8 g, soluble starch 10 g, methi ... | 1989 | 18588016 |
| high-level expression of tuberous root storage protein genes of sweet potato in stems of plantlets grown in vitro on sucrose medium. | sporamin, the tuberous root storage protein of the sweet potato, accounts for about 60 to 80% of the total soluble protein of this organ. the amount of sporamin present in other organs is very low, or even not detectable, in the normal field-grown plants. however, the stem of sweet potato plantlets grown axenically on agar medium containing sucrose was found to accumulate large amounts of sporamin. two-dimensional gel electrophoretic profiles of sporamin precursors synthesized in vitro by poly(a ... | 1990 | 2102838 |
| the plant mitochondrial f1-atpase. the identity of the delta' (20 kda) subunit. | the n-terminal amino acid sequence of the 20 kda (delta') subunit of the turnip (brassica napus l.) mitochondrial f1-atpase has been determined. comparison of the sequence obtained with those of the epsilon subunits of chloroplast cf1, e. coli f1 and the delta subunit of bovine f1 shows that the turnip delta' subunit is another member of this family of homologous proteins. the delta' subunit of sweet potato f1-atpase [(1989) j. biol. chem. 264, 3183-3186] is very similar to the turnip sequence a ... | 1990 | 2147662 |
| alteration of the properties of aspergillus sp. k-27 glucoamylase on limited proteolysis with subtilisin. | an active derivative (mol. wt. 48,000) of aspergillus sp. k-27 glucoamylase (mol. wt. 76,000) was obtained by limited proteolysis with subtilisin. the amino acid sequences of native and modified enzymes at the n-termini were ala-gly-gly-thr-leu-asp and ala-val-leu, respectively. the proteolysis greatly decreased the affinity of the enzyme for amylopectin and glycogen, but not for oligosaccharides. it also reduced the ability of the enzyme to degrade raw starch, abolished the ability of the enzym ... | 1990 | 2224899 |
| vacuolar targeting and posttranslational processing of the precursor to the sweet potato tuberous root storage protein in heterologous plant cells. | sporamin, the tuberous root storage protein of the sweet potato, which is localized in vacuoles, is synthesized as a prepro-precursor with an n-terminal sequence of amino acids that includes a signal peptide and an additional pro-segment of 16 amino acids. a full-length cdna for sporamin was placed downstream of the 35 s promoter of cauliflower mosaic virus and introduced into tobacco and sunflower genomes by ti plasmid-mediated transformation. a polypeptide of nearly the same size as mature spo ... | 1990 | 2246259 |
| characterization of a cdna encoding cottonseed catalase. | a 1.7 kb cdna clone was isolated from our lambda gt11 library constructed from poly(a) rna of 24-h-old cotyledons. the cdna encodes a full-length catalase peptide (492 amino acid residues). the calculated molecular mass is 56,800, similar to that determined for purified enzyme (57,000 sds-page). among higher plant catalases, this cotton catalase shows the highest amino acid sequence identity (85%) to the subunit of homotetrameric maize cat 1, a developmental counterpart to the homotetrameric cat ... | 1990 | 2364113 |
| optimizing sweet potato [ipomoea batatas (l.) lam.] root and plantlet formation by selection of proper embryo developmental stage and size, and gel type for fluidized sowing. | potassium starch polyacrylamide, potassium acrylate, a copolymer of potassium acrylate and acrylamide, and hydroxyethylcellulose carrier gels were tested to find a fluid drilling material suited for synthetic seeding of sweet potato (ipomoea batatas (l.) lam.) somatic embryos. somatic embryo developmental stage and size, and maturation (incubation) time were also evaluated to improve plantlet formation. all embryos suspended in the fluidized hydroxyethylcellulose gel were viable after six days a ... | 1990 | 24227054 |
| cytological analysis of tetraploid hybrids between sweet potato and diploid ipomoea trifida (h. b. k.) don. | tetraploid f1 hybrids between ipomoea batatas, sweet potato (2n = 6x = ca. 90), and diploid (2n = 2x = 30) i. trifida (h. b. k.) don. showed various degrees of fertility reduction. the present study aimed to clarify its causes by cytological analysis of meiotic chromosome behavior in the diploid and sweet potato parents and their tetraploid hybrids. the diploid parents showed exclusively 15 bivalents, and the sweet potato parents exhibited almost perfect chromosome pairing along with predominant ... | 1990 | 24221067 |
| the inheritance of β-amylase null in storage roots of sweet potato,ipomoea batatas (l.) lam. | several sweet potato genotypes were found to lack completely or to have only traces ofβ-amylase in their storage roots. such genotypes do not increase in sweetness during cooking because, without a sufficient amount ofβ-amylase, the normal hydrolysis of starch to maltose does not occur in the cooking process. in order to study the inheritance of this biochemical variant in the genotype, 41 families were generated. the following conclusions were drawn from analyzing these families. (1) this trait ... | 1990 | 24226356 |
| use of ipomoea trifida germ plasm for sweet potato improvement. 3. development of 4x interspecific hybrids between ipomoea batatas (l.) lam. (2n=6x=90) and i. trifida (h.b.k) g. don. (2n=2x=30) as storage-root initiators for wild species. | more than 28,000 pollinations were carried out between 5 ipomoea batatas and 41 diploid i. trifida accessions of diverse origins to obtain 4x interspecific hybrids. from the resultant 730 seeds, 248 plants were finally obtained. ploidy level determination of the progeny showed unexpected results: 52 individuals were hexaploid, 5 were pentaploid, 190 were tetraploid, as expected, and one was not determined. the existence of 5x and 6x progenies from 6x x 2x crosses not only confirmed the presence ... | 1991 | 24202352 |
| [chemical constituents of ipomola batatas lam]. | three chemical compounds isolated from the leaves and stems of ipomoea batatas were identified as fumaric acid, succinic acid and 7,3',4'-trimethylquercetin by chemical and spectroscopic analysis. in this paper, an analysis of the amino-acids of ipomoea batatas is also reported. | 1991 | 1804202 |
| sucrose-induced accumulation of beta-amylase occurs concomitant with the accumulation of starch and sporamin in leaf-petiole cuttings of sweet potato. | beta-amylase of sweet potato (ipomoea batatas l.), which constitutes about 5% of the total soluble protein of the tuberous root, is absent or is present in only small amounts in organs other than the tuberous roots of the normal, field-grown plants. however, when leaf-petiole cuttings from such plants were supplied with a solution that contained sucrose, the accumulation of beta-amylase was induced in both leaf and petiole portions of the explants. the sucrose-induced accumulation of beta-amylas ... | 1991 | 16668273 |
| purification and characterization of p-coumaroyl-d-glucose hydroxylase of sweet potato (ipomoea batatas) roots. | p-coumaroyl-d-glucose hydroxylase in sweet potato (ipomoea batatas lam.) has been purified to apparent electrophoretic homogeneity using a combination of anion-and cation-exchange, hydrophobic and gel filtration chromatography. the purified enzyme was a monomer with a molecular weight of 33,000 and pi of 8.3. the purified enzyme showed not only hydroxylase activity but also polyphenol oxidase activity. l-ascorbic acid was the best electron donor for the hydroxylation reaction, which had an optim ... | 1991 | 1989492 |
| the effect of diet on microfaunal population and function in the caecum of a subterranean naked mole-rat, heterocephalus glaber. | the effect of dietary fibre and starch content on digestibility, microfaunal population and caecal function was investigated in a subterranean mole-rat, heterocephalus glaber (rodentia). mole-rats were fed on a diet of either sweet potato (neutral-detergent fibre (ndf) 65 g/kg dry matter (dm), starch 638 g/kg dm) or carrot (ndf 157 g/kg dm, starch 258.7 g/kg dm) for 4 weeks. daily intake and faecal output were monitored. thereafter caecal microfaunal population, density and function were assesse ... | 1991 | 1645994 |
| studies on aldose reductase inhibitors from natural products. iv. constituents and aldose reductase inhibitory effect of chrysanthemum morifolium, bixa orellana and ipomoea batatas. | the hot water extracts of chrysanthemum morifolium, bixa orellana and ipomoea batatas, were found to have potent inhibitory activity towards lens aldose reductase (ar). ellagic acid (4) was isolated from c. morifolium and i. batatas, isoscutellarein (7) from b. orellana and 3,5-dicaffeoylquinic acid (10) from i. batatas, respectively, as potent inhibitors. | 1991 | 1814628 |
| kinetics and mathematical model of ethanol formation by immobilized yeast cells. | kinetics of the ethanol fermentation with the immobilized k yeast cells was studied both experimentally and theoretically. a kinetic model consisting of 6 equations was proposed. in this model the combined inhibition, i.e., glucose in uncompetitive inhibition and ethanol in noncompetitive inhibition with irreversible performance, was taken into consideration. calculated values agreed approximately with the experimental results, and this model was found to be of practical use. the maximum average ... | 1991 | 1823593 |
| molecular cloning and expression in escherichia coli of cdna encoding the subunit of sweet potato beta-amylase. | tuberous roots of the sweet potato are unusually rich in beta-amylase, and the beta-amylase polypeptides account for about 5% of the total soluble protein of the organ. unlike beta-amylases from other origins, the sweet potato beta-amylase is a tetramer of identical subunits, and it also bears starch phosphorylase-inhibitor activity. a cdna for the subunit of sweet potato beta-amylase was obtained by immunological screening of an expression cdna library constructed by the vector-primer and linke ... | 1991 | 1837016 |
| ubiquinol-cytochrome c oxidoreductase of higher plants. isolation and characterization of the bc1 complex from potato tuber mitochondria. | a procedure is described for isolation of active ubiquinol-cytochrome c oxidoreductase (bc1 complex) from potato tuber mitochondria using dodecyl maltoside extraction and ion exchange chromatography. the same procedure works well with mitochondria from red beet and sweet potato. the potato complex has at least 10 subunits resolvable by gel electrophoresis in the presence of dodecyl sulfate. the fifth subunit carries covalently bound heme. the two largest ("core") subunits either show heterogenei ... | 1991 | 1851164 |
| propeptide of a precursor to a plant vacuolar protein required for vacuolar targeting. | sporamin is a protein without glycans that accumulates in large quantities in the vacuoles of the tuberous root of the sweet potato. it is synthesized as a prepro precursor with an n-terminal extension composed of a 21-amino-acid signal peptide and a 16-amino-acid propeptide. a total of 48 base pairs, corresponding to the nucleotide sequence that encodes the propeptide, was deleted from a cdna clone for sporamin. this delta pro mutant sequence, as well as the sequence of the wild-type sporamin c ... | 1991 | 1992474 |
| high-level expression of a sweet potato sporamin gene promoter: beta-glucuronidase (gus) fusion gene in the stems of transgenic tobacco plants is conferred by multiple cell type-specific regulatory elements. | genes coding for sporamin, the most abundant protein of the tuberous root of the sweet potato, are expressed at a high levels in the stems of plantlets cultured axenically on sucrose-containing medium. their expression is also induced in leaf-petiole explants by high concentrations of sucrose. a fusion gene comprising of the 1 kb 5' upstream region of the gspo-a1 gene coding for the a-type sporamin and the coding sequence of bacterial beta-glucuronidase (gus) was introduced into the tobacco geno ... | 1991 | 2017135 |
| primary structure of sweet potato starch phosphorylase deduced from its cdna sequence. | sweet potato (ipomoea batatas) starch phosphorylase cdna clones were isolated by screening an expression library prepared from the young root poly(a)(+) rna successively with an antiserum, a monoclonal antibody, and a specific oligonucleotide probe. one cdna clone had 3292 nucleotide residues in which was contained an open reading frame coding for 955 amino acids. this sequence was compared with those of potato (916 residues plus 50-residue putative transit peptide) and rabbit muscle (841 residu ... | 1991 | 16668119 |
| genetic interrelatedness among clover proliferation mycoplasmalike organisms (mlos) and other mlos investigated by nucleic acid hybridization and restriction fragment length polymorphism analyses. | dna was isolated from clover proliferation (cp) mycoplasmalike organism (mlo)-diseased periwinkle plants (catharanthus roseus (l.) g. don.) and cloned into psp6 plasmid vectors. cp mlo-specific recombinant dna clones were biotin labeled and used as probes in dot hybridization and restriction fragment length polymorphism analyses to study the genetic interrelatedness among cp mlo and other mlos, including potato witches'-broom (pwb) mlo. results from dot hybridization analyses indicated that both ... | 1991 | 16348604 |
| isolation of substance from sweet potato (ipomoea batatas) periderm tissue that inhibits seed germination. | chromatographic procedures were used to isolate inhibitors of seed germination from sweet potato root periderm tissue. the inhibitory activity of all fractions was monitored using a proso millet seed germination bioassay. a single hplc peak, representing approximately 1.2% of the periderm dry weight, accounted for most of the inhibitory activity. the active fraction was labile in methanolic solution. further fractionation of this peak by hplc methods was not successful. in vitro seed germination ... | 1991 | 24259077 |
| sugar-dependent expression of the chs-a gene for chalcone synthase from petunia in transgenic arabidopsis. | transgenic arabidopsis thaliana plants were constructed by introduction of a fusion of the gene for beta-glucuronidase (gus) to the chs-a gene, which is one of the two genes for chalcone synthase that are actively expressed in the floral organs of petunia. the expression of the fusion gene chs-a::gus was low in transgenic arabidopsis plantlets, but it was enhanced when plantlets or detached leaves were transferred to a medium that contained 0.3 molar sucrose, glucose, or fructose. no enhancement ... | 1991 | 16668565 |
| sorghum brewing using sweet potato enzymic flour to increase saccharification. | the diastatic activity of three sweet potato varieties was principally due to \-amylase. substitution of sorghum malt with sweet potato at 20% (w/v) gave a higher activity than an all-sorghum malt. maltose in the sorghum/potato wort was 50 mg/ml, similar to that in barley malt. the free alpha amino nitrogen of the sorghum/potato worts was lower than that of the all-sorghum malt but was still within the range needed for yeast growth. incubation of the potato enzymic extract with isolated sorghum ... | 1992 | 24425566 |
| nematode numbers and crop yield in a fenamiphos-treated sweet corn-sweet potato-vetch cropping system. | nematode population densities and yield of sweet corn and sweet potato as affected by the nematicide fenamiphos, in a sweet corn-sweet potato-vetch cropping system, were determined in a 5-year test (1981-85). sweet potato was the best host of meloidogyne incognita of these three crops. fenamiphos 15g (6.7 kg a.i./ha) incorporated broadcast in the top 15 cm of the soil layer before planting of each crop increased (p </= 0.05) yields of sweet corn in 1981 and 1982 and sweet potato number 1 grade i ... | 1992 | 19283032 |
| phenylalanine ammonia-lyase from loblolly pine : purification of the enzyme and isolation of complementary dna clones. | phenylalanine ammonia-lyase (ec 4.3.1.5) has been purified from differentiating secondary xylem of loblolly pine (pinus taeda l.). native molecular weight of the enzyme was estimated to be 280,000, with a subunit molecular weight of 74,000; isoelectric point, 5.8; and michaelis constant for i-phenylalanine, 27 micromolar. no evidence was obtained for the existence of isoforms of the enzyme, nor for negative cooperativity of substrate binding. polyclonal antibodies were raised against the phenyla ... | 1992 | 16668639 |
| construction of hybridomas secreting monoclonal antibody against sweet potato feathery mottle virus and use of antibody for detection of spfmv. | two hybridomas stably secreting monoclonal antibodies (mcab) against sweet potato feathery mottle virus (spfmv) were constructed by fusing mouse myeloma (sp2/0-ag14) with mouse splenocytes immunized with spfmv-lf. the mcab secreted by the two hybridomas specifically reacted with both spfmv-lf (china strain) and spfmv-rc (american strain) in dot-blot-elisa. a large amount of mcab was produced with balb/c mice. the titer of hybridoma cultured supernatant was 1:256, and that of the ascitic fluids w ... | 1992 | 1343832 |
| a nuclear gene encoding beta-amylase of sweet potato. | a nuclear amyb gene from sweet potato encoding beta-amylase (beta amy) that is abundant in tuberous roots and inducible in other organs by an exogenous supply of sucrose or polygalacturonic acid, was isolated and characterized. genomic southern blot hybridization, restriction maps of independently isolated phage lambda genomic clones, and the nucleotide sequence of amyb compared with that of the cdna, all suggested that beta amy of sweet potato is encoded by a gene that is present in a single co ... | 1992 | 1383095 |
| a simple, rapid method for purification of epsilon-subunit, coupling factor 6, subunit d, and subunit e from rat liver h(+)-atp synthase and determination of the complete amino acid sequence of epsilon-subunit. | the rat liver mitochondrial epsilon-subunit, coupling factor 6, subunit d, and subunit e of h(+)-atp synthase, which are all extra subunits with no counterparts in escherichia coli, were purified by reverse-phase high performance liquid chromatography. the complete amino acid sequence of the rat epsilon-subunit was determined by automated edman degradation of the whole protein and derived peptides. the protein contains 50 amino acids and has a molecular mass of 5635 kda. it is a basic hydrophili ... | 1992 | 1429613 |
| maternity and medicinal plants in vanuatu. i. the cycle of reproduction. | ethnobotanical data collection to select pharmacologically active species was carried out within a clearly defined therapeutic context: those plants used during the course of a woman's reproductive life. extensive bibliographical and field data collection and cross-examination of the information thus gathered have provided us with a clearer picture of the effectiveness of these plant species. various concepts, behaviours and practices relating to menstruation, pregnancy, birth and birth control ... | 1992 | 1453707 |
| the nuclear factor sp8bf binds to the 5'-upstream regions of three different genes coding for major proteins of sweet potato tuberous roots. | sporamin and beta-amylase are two major proteins of tuberous roots of sweet potato, and expression of genes coding for sporamin and beta-amylase is induced concomitantly in leaves with the petioles attached by exogenous supply of sucrose or polygalacturonic acid. we have used a dnase i footprinting assay to characterize nuclear factors that bind to the 5'-upstream regions of gspo-a1, gspo-b1 and g beta-amy genes that encode a-type sporamin, b-type sporamin and the subunit of beta-amylase, respec ... | 1992 | 1531033 |
| dietary intake of vitamin a in african pre-school children in umlazi, south africa. | vitamin a deficiency has been shown to have an impact on morbidity and mortality and therefore prevalence rates are important for public health policy. we report the use of a 7-day recall, dietary questionnaire, which was validated and administered to 203 randomly selected urban african pre-school children in umlazi, south africa. common vitamin a containing foods eaten were leafy green vegetables, pumpkin, sweet potato, mangoes and chicken liver. although the mean daily intake of vitamin a was ... | 1992 | 1587704 |
| dna relatedness among strains of the sweet potato pathogen streptomyces ipomoea (person and martin 1940) waksman and henrici 1948. | dna relatedness among 28 putative strains of streptomyces ipomoea from geographically diverse locations and the type strain, nrrl b-12321, was determined spectrophotometrically. the data confirm that these 28 strains are not closely related genetically to the plant-pathogenic species streptomyces scabies (39% dna relatedness) or streptomyces acidiscabies (17% dna relatedness) or any other major blue-spored streptomyces species (less than 30% dna relatedness). of the 29 strains examined, 4 could ... | 1992 | 1610178 |
| dna-dna hybridization in the systematics of streptomyces. | the dna relatedness among strains in several different phenotypically defined streptomyces species clusters was evaluated. it was found that the data from dna-relatedness studies do not necessarily agree with the clustering generated using numerical taxonomic techniques. a study of the morphologically heterogeneous 's. cyaneus' cluster showed that morphological criteria traditionally used to classify and identify streptomyces species still have value, since strains in dna-relatedness cluster gro ... | 1992 | 1612444 |
| acute interstitial pneumonia in cattle associated with consumption of mould-damaged sweet potatoes (ipomoea batatas). | 1992 | 1632730 | |
| resin glycosides. xv. simonins i-v, ether-soluble resin glycosides (jalapins) from the roots of ipomoea batatas (cv. simon). | five new ether-soluble resin glycosides (jalapins), simonins i-v, have been isolated from the roots of ipomoea batatas and characterized on the bases of chemical and spectral data. simonin i is the first example of resin glycoside with aromatic acid (trans-cinnamic acid) as a component organic acid. | 1992 | 1294318 |
| developmental changes induced by gamma irradiation in ipomoea batatas l. lam (sweet potato). | storage roots of ipomoea batatas l. lam were exposed to gamma irradiation at 90, 180, and 240 gy. the two highest doses caused a delay in the initiation of rooting and especially of shooting; they also caused formation of rosette-like, stemless shoots without apical meristems. the storage roots irradiated with 90 gy did form stems, but 50% of them had no apical meristem. the irradiated storage roots produced absorbent roots which developed shoots after 70 days of growth. nonirradiated storage ro ... | 1992 | 1438705 |
| induction of expression of genes coding for sporamin and beta-amylase by polygalacturonic acid in leaf-petiole cuttings of sweet potato. | sporamin and beta-amylase are two major proteins of tuberous storage root of sweet potato (ipomoea batatas) and their accumulation can be induced concomitantly with the accumulation of starch in leaves and petioles by sucrose (k nakamura, m ohto, n yoshida, k nakamura [1991] plant physiol 96: 902-909). although mechanical wounding of leaves of sweet potato only occasionally induced the expression of sporamin and beta-amylase genes, their expression could be reproducibly induced in leaf-petiole c ... | 1992 | 16668901 |
| cryopreservation of sweet potato (ipomoea batatas [l.] lam.) shoot tips by vitrification. | vitrification is a technically simple method for cryopreserving plant germplasm, requiring only the application of suitable cryoprotectants and rapid cooling rates. sweetpotato (ipomoea batatas [l.] lam.) shoot tips obtained from in vitro plants survived liquid nitrogen (-196°c) exposure following a vitrification-inducing pretreatment. shoot tips were treated in a stepwise manner with a vitrification solution containing 30% glycerol, 15% ethylene glycol and 15% dimethylsulfoxide in growth medium ... | 1992 | 24202980 |
| noninvasive evaluation of somatic embryogenesis. | callus suspension cultures of ipomea batates poir. cv. white star were grown in an airlift bioreactor. a machine vision system was used to monitor nondestructively callus growth during a 10 day culture period. growth data obtained with this system included the overall reactor population and population estimates for the 200-1200-microm fractions at 200-microm intervals. a model of callus growth was developed to explain the mechanics of callus enlargement. the model was based on the assumptions th ... | 1992 | 18600958 |
| high performance liquid chromatography analysis of carbohydrates of cotton-phloem sap and of honeydew produced by bemisia tabaci feeding on cotton. | phloem sap from cotton (gossypium hirsutum l.) was collected from young and mature leaves by the aphid-stylet technique. exudate was analyzed for carbohydrates by hplc using sensitive pulsed amperometric detection. the predominant carbohydrate present (>90%) was identified as sucrose. a second, unidentified compound that was not one of the more commonly translocated sugars was detected in mature leaves. carbohydrates in honeydew produced by the sweet-potato whitefly (bemisia tabaci [genn.]) feed ... | 1992 | 16668706 |
| isolation and primary structure of proteinase inhibitors from erythrina variegata (linn.) var. orientalis seeds. | the kunitz-type trypsin inhibitors, etia and etib, and chymotrypsin inhibitor eci were isolated from the seeds of erythrina variegata. the proteins were extracted from a defatted meal of seeds with 10 mm phosphate buffer, ph 7.2, containing 0.15 m nacl, and purified by deae-cellulose and q-sepharose column chromatographies. the stoichiometry of trypsin inhibitors with trypsin was estimated to be 1:1, while that of chymotrypsin inhibitor with chymotrypsin was 1:2, judging from the titration patte ... | 1992 | 1369077 |
| growing root, tuber and nut crops hydroponically for celss. | among the crops selected by the national aeronautics and space administration for growth in controlled ecological life support systems are four that have subsurface edible parts -- potatoes, sweet potatoes, sugar beets and peanuts. these crops have been produced in open and closed (recirculating), solid media and liquid, hydroponic systems. fluorescent , fluorescent plus incandescent and high pressure sodium plus metal halide lamps have proven to be effective light sources. continuous light with ... | 1992 | 11537058 |
| affinity purification of beta-amylases originating from plant using cyclomaltohexaose-immobilized sepharose 6b in the presence of ammonium sulfate. | this paper reports a novel method of affinity purification of soybean and barley beta-amylase on cyclomaltohexaose-immobilized sepharose. until now, it has been shown that sweet potato beta-amylase can be purified using the above absorbent but beta-amylases from soybean and barley seeds cannot. we found that soybean and barley beta-amylase becomes adsorbed specifically on the above absorbent if it is in solution with 1 to 2 m ammonium sulfate, and the adsorbed enzyme can be easily eluted with a ... | 1993 | 8374302 |
| cdnas encoding for storage proteins in the tubers of taro (colocasia esculenta schott). | two major protein groups of taro (colocasia esculenta) tuber were purified, and their antisera were used for the screening of the cdna library constructed from poly(a)+ rna of taro tuber. a cdna clone obtained by screening with an anti-12 kd protein antiserum had an insert 1058 bp-long, and an open reading frame for a peptide of 268 amino acids. the analyses of the n-terminal amino acid sequence and in vitro translation product suggested that the protein was synthesized as a peptide with a molec ... | 1993 | 8251188 |
| cloning and characterization of a cdna encoding the cytosolic copper/zinc-superoxide dismutase from sweet potato tuberous root. | a full-length cdna clone encoding a putative copper/zinc-superoxide dismutase (sod) of sweet potato, ipomoea batatas (l.) lam. cv tainong 57, was isolated from a cdna library constructed in lambda gt10 from tuber root mrna. nucleotide sequence analysis of this cdna clone revealed that it comprises a complete open reading frame coding for 152 amino acid residues. the deduced amino acid sequence showed higher homology (78-86%) with the sequence of the cytosolic sod than that of the chloroplast sod ... | 1993 | 8251645 |
| sweet potato beta-amylase. primary structure and identification of the active-site glutamyl residue. | the complete amino acid sequence of a subunit of sweet potato beta-amylase, a homotetramer, was established by sequence analysis of peptides obtained by digestions with achromobacter protease i and staphylococcus aureus v8 protease and by cyanogen bromide cleavage of the s-carboxymethylated subunit. the subunit of the enzyme is a single polypeptide consisting of 498 amino acid residues. it showed 50-60% identity in the amino acid sequence with those of beta-amylases from soybean and barley, whil ... | 1993 | 8103452 |
| identification of a sweet potato feathery mottle virus isolate from china (spfmv-ch) by the polymerase chain reaction with degenerate primers. | four degenerate oligonucleotide primers derived from conserved regions of the genome of potyviruses have been designed. a combined assay of reverse transcription and the polymerase chain reaction utilizing these primers on total rna extracted from ipomoea purpurea infected with a sweet potato feathery mottle virus isolate from china (spfmv-ch), amplified a 1.35 kb and a 830 bp fragment. these amplified fragments were cloned into the bluescript plasmid and sequenced. the comparison of the sequenc ... | 1993 | 8113341 |
| the presequence of a precursor to the delta-subunit of sweet potato mitochondrial f1atpase is not sufficient for the transport of beta-glucuronidase (gus) into mitochondria of tobacco, rice and yeast cells. | a precursor to the delta-subunit of sweet potato mitochondrial f1atpase (pre-f1 delta) has an amino-terminal (n-terminal) presequence of 45 amino acid residues and its n-terminal 18 residues may form an amphiphilic alpha-helix, which is typical of mitochondrial targeting signals [kimura et al. (1990) j. biol. chem. 265: 6079]. fusion genes consisting of sequences that encoded the 25 (dg25), 46 (dg46) and 73 (dg73) n-terminal amino acids from pre-f1 delta fused to the n-terminus of the coding seq ... | 1993 | 8199776 |
| sweet potato feathery mottle potyvirus (c1 isolate) virion and rna purification. | a procedure for the purification of a peruvian isolate (c1) of sweet potato feathery mottle potyvirus (spfmv) and infective rna has been developed. the use of hepes [n-(2-hydroxyethyl)piperazine-n'-2-ethanesulfonic acid] buffer containing urea and sodium edta as a base for tissue extraction and virus suspension enabled good yields of virus (35-50 mg/100 g) to be obtained from nicotiana benthamiana l. domin. a short rna isolation procedure yielded infectious rna, from which ds cdna of nearly geno ... | 1993 | 8227274 |
| colocalization of barley lectin and sporamin in vacuoles of transgenic tobacco plants. | various targeting motifs have been identified for plant proteins delivered to the vacuole. for barley (hordeum vulgare) lectin, a typical gramineae lectin and defense-related protein, the vacuolar information is contained in a carboxyl-terminal propeptide. in contrast, the vacuolar targeting information of sporamin, a storage protein from the tuberous roots of the sweet potato (ipomoea batatas), is encoded in an amino-terminal propeptide. both proteins were expressed simultaneously in transgenic ... | 1993 | 8278507 |
| molecular cloning and characterization of cdnas for the gamma- and epsilon-subunits of mitochondrial f1f0 atp synthase from the sweet potato. | mitochondrial f1f0 atp synthases purified from dicotyledonous plants contain six different subunits named alpha, beta, gamma, delta, delta', and epsilon. our previous n-terminal amino acid sequence analyses indicated that the gamma- and epsilon-subunits of the sweet potato mitochondrial f1 correspond to the gamma- and epsilon-subunits of animal mitochondrial f1, respectively (kimura, t., nakamura, k., kajiura, h., hattori, h., nelson, n., and asahi, t. (1989) j. biol. chem. 264, 3183-3186). a cd ... | 1993 | 8349605 |
| lung injury by furanoterpenoids isolated from fusarium solani infected sweet potato, ipomea batatas. | intraperitoneal administration (1 mg/kg body/wt./day for 21 days) of crude extract of furanoterpenoids, isolated from f. solani damaged i. batatas caused pulmonary oedema in albino rats. the elevated broncho alveolar lavage (bal) angiotensin converting enzyme (ace), lactate dehydrogenase (ldh) and protein levels indicated lung damage. the estimation of pulmonary extracellular surfactant phospholipids showed an alteration in various phospholipid fractions. | 1993 | 8359843 |
| sensory evaluation and nutrient composition of weaning food from pregelatinized maize-sweet potato mixtures. | pregelatinized maize-sweet potato mix, fortified with soybeans and groundnut flours, was evaluated for its acceptability as a weaning food and for nutrient composition. an acceptable product had 14.3, 6.4, 2.4 and 67.4%, respectively, for its protein, fat, ash and carbohydrate contents. values for calcium, phosphorus and and iron contents were 59.6, 187.5 and 2.4 mg/100 g, respectively, while the most predominant fatty acids were linoleic, oleic and palmitic acids. the product, which had a chemi ... | 1993 | 8378272 |
| cloning of the beta-amylase gene from bacillus cereus and characteristics of the primary structure of the enzyme. | the gene encoding the beta-amylase of bacillus cereus bq10-s1 (spoii) was cloned into escherichia coli jm 109. a sequenced dna fragment of 2,001 bp contains the beta-amylase gene. the n-terminal sequences (avngkg mnpdykaylmaplkki), the c-terminal sequences (shtssw), and the amino acid sequences of the five regions in the beta-amylase molecules were determined. the mature beta-amylase contains 514 amino acid residues with a molecular mass of 57,885 da. the amino acid sequence homology with those ... | 1993 | 8434930 |
| reproductive and damage potentials of two populations of rotylenchulus reniformis on sweetpotato and related comparisons with meloidogyne javanica on tomato. | two rotylenchulus reniformis populations (north carolina and georgia) were compared on sweetpotato and tomato. 'beauregard' sweetpotato and 'better boy' and 'marion' tomato were excellent hosts for both r. reniformis populations. on beauregard sweetpotato, the two populations did not differ in fecundity; however, on both tomato cultivars, the georgia population reproduced at a higher rate than the north carolina population (p </= 0.05). meloidogyne javanica reproduction was higher (p </= 0.05) o ... | 1993 | 19279849 |
| determination of base specificity of multiple ribonucleases from crude samples. | ribonucleases are widely found on the tissues of living organisms, but the functions of individual ribonucleases are not clear. to facilitate characterization of individual ribonucleases, i have developed a rapid method to separate and identify each ribonuclease from a crude sample by gel electrophoresis instead of by time-consuming purification steps. the ribonucleases in a crude sample are first separated by rna-cast sds-polyacrylamide gel electrophoresis and then eluted from the gel after eth ... | 1994 | 7565655 |
| [epidemiology and control of hookworm infection in fujian province]. | fujian province has been known to be endemic for mixed hookworm infection with ancylostoma duodenale as the predominant species. the infection rate was 50% before 1980. as a result of mass treatment starting from 1980, the infection rate was decreased in most areas. and, infection foci were changed from sweet potato fields to the banana, sweatcane and asparagus fields where the infection rate tended to increase. it is stressed that the current endemic characteristics of hookworm infection should ... | 1994 | 7720205 |
| nucleotide sequence at the 3'-terminal region of sweet potato feathery mottle virus (ordinary strain, spfmv-o) rna. | cdna encoding the 3'-terminal region of sweet potato feathery mottle virus (ordinary strain, spfmv-o) rna was cloned and sequenced. the 2324-bp cdna contained an open reading frame (orf) of 2066 bp followed by an 3' non-coding region and a poly(a) region. the orf covered the coat protein and the carboxy terminus of the nuclear inclusion 'b' protein (nib). | 1994 | 7764983 |
| inhibitors of protein phosphatases 1 and 2a block the sugar-inducible gene expression in plants. | genes coding for two major proteins of the tuberous root of sweet potato (ipomoea batatas), namely, sporamin and [beta]-amylase, are inducible in leaves and petioles when they are supplied with high concentrations of sucrose or other metabolizable sugars, such as glucose and fructose, and the accumulation of a large amount of starch accompanies this induction. three inhibitors of protein phosphatases 1 (pp1) and 2a (pp2a), namely, okadaic acid, microcystin-lr, and calyculin a, strongly inhibited ... | 1994 | 12232349 |
| the relationship between electron flux and the redox poise of the quinone pool in plant mitochondria. interplay between quinol-oxidizing and quinone-reducing pathways. | the dependence of electron flux through quinone-reducing and quinol-oxidizing pathways on the redox state of the ubiquinone (q) pool was investigated in plant mitochondria isolated from potato (solanum tuberosum cv. bintje, fresh tissue and callus), sweet potato (ipomoea batatas) and arum italicum. we have determined the redox state of the q pool with two different methods, the q-electrode and q-extraction techniques. although results from the two techniques agree well, in all tissues tested (wi ... | 1994 | 7813462 |
| interpretation of randomly amplified polymorphic dna marker data for fingerprinting sweet potato (ipomoea batatas l.) genotypes. | in this paper we present a method for the generation of randomly amplified polymorphic dna (rapd) markers for sweet potato. these were applied to produce genetic fingerprints of six clonal cultivars and to estimate genetic distances between these cultivars. the level of polymorphism within the species was extremely high. from the 36-decamer random primers used, 170 fragments were amplified, of which 132 (77.6%) were polymorphic. ten primers resulted in no detected amplification. of the remaining ... | 1994 | 24186015 |
| isozyme modifications and plant regeneration through somatic embryogenesis in sweet potato (ipomoea batatas (l.) lam.). | the potential of somatic embryogenesis was evaluated for 10 cultivars of sweet potato through extensive embryogenic response and isozyme analysis. embryogenic callus was induced by incubating lateral buds on murashige and skoog medium containing 10 μm 2,4-dichlorophenoxyacetic acid for 6-8 weeks. the frequency of embryogenic response was low, and varied with genotypes, ranging from 0 to 17%. embryo to plantlet formation could be enhanced by the use of the combination of 2,4-dichlorophenoxyacetic ... | 1994 | 24194021 |
| analysis of genetic diversity in a sweetpotato (ipomoea batatas) germplasm collection using dna amplification fingerprinting. | a dna amplification fingerprinting (daf) approach was employed to develop individual-specific profiles and analyze genetic relationships among 73 plant introductions of sweetpotato (ipomoea batatas (l.) lam.) including unadapted lines from around the world and a few selected u.s.a. cultivars. reliable and informative fingerprint profiles were obtained employing single octamer primers and stoffel fragment taq polymerase in the polymerase chain reaction, polyacrylamide-based vinyl polymer for elec ... | 1995 | 8537002 |
| purification and properties of l-galactono-gamma-lactone dehydrogenase, a key enzyme for ascorbic acid biosynthesis, from sweet potato roots. | l-galactono-gamma-lactone dehydrogenase (l-galactono-gamma-lactone:ferricytochrome c oxidoreductase [ec 1.3.2.3], gldhase) which catalyzes the terminal step in the biosynthesis of l-ascorbic acid (asa) has been purified from roots of sweet potato (ipomoea batatas l., cv. kintoki). highly purified preparation of the gldhase was obtained by three column chromatography steps with a recovery of ca. 1%, after solubilization from mitochondria in sweet potato roots. sds-page exhibited a single band at ... | 1995 | 7775377 |
| cryopreservation of embryogenic tissue of sweet potato (ipomoea batatas): use of sucrose and dehydration for cryoprotection. | embryogenic tissue of two sweet potato (ipomoea batatas (l) lam) genotypes, tib 10 and nemanete (nem), was established from in vitro axillary meristems on murashige and skoog (1962) media supplemented with 2,4-dichlorophenoxyacetic acid and 2,4,5-trichlorophenoxyacetic acid respectively. embryogenic aggregates of approximately 1.5-2.0 mm in diameter were subjected to a rapid or a two-step freezing protocol in liquid nitrogen following alginate encapsulation, sucrose preculture and varying degree ... | 1995 | 24185787 |
| analysis of plant harvest indices for bioregenerative life support systems. | harvest indices, which are measures of the ratio of edible to total plant weight, are redefined to include edible sugars derived from enzymatic hydrolysis of the cellulose content of inedible plant components. compositional analysis and carbohydrate contents of rapeseed, rice, soybeans, cowpea, wheat, sweet potato, white potato, and lettuce were analyzed to develop such generalized harvest indices. cellulose conversion is shown to extend considerably the food available from plants otherwise grow ... | 1995 | 11541096 |
| sugar-induced increase of calcium-dependent protein kinases associated with the plasma membrane in leaf tissues of tobacco. | the sugar-inducible expression of genes for sporamin and [beta]-amylase in leaf explants of sweet potato (ipomoea batatas) and that of a [beta]-glucuronidase-fusion gene, with the promoter of the gene for [beta]-amylase in leaves of tobacco (nicotiana tabacum), requires ca2+ signaling (m. ohto, k. hayashi, m. isobe, k. nakamura [1995] plant j 7: 297-307), and it was inhibited by staurosporin and k252a, inhibitors of protein kinases. autophosphorylation activities of several potential protein kin ... | 1995 | 12228646 |
| crystallization, molecular replacement solution, and refinement of tetrameric beta-amylase from sweet potato. | sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. its subunit consists of 498 amino acid residues (mr 55,880). it has been crystallized at room temperature using polyethylene glycol 1500 as precipitant. the crystals, growing to dimensions of 0.4 mm x 0.4 mm x 1.0 mm within 2 weeks, belong to the tetragonal space group p4(2)2(1)2 with unit cell dimensions of a = b = 129.63 a and c = 68.42 a. the asymmetric unit contains 1 subunit ... | 1995 | 7777485 |
| a tobacco protein kinase, npk2, has a domain homologous to a domain found in activators of mitogen-activated protein kinases (mapkks). | a cdna (cnpk2) that encodes a protein of 518 amino acids was isolated from a library prepared from poly(a)+ rnas of tobacco cells in suspension culture. the n-terminal half of the predicted npk2 protein is similar in amino acid sequence to the catalytic domains of kinases that activate mitogen-activated protein kinases (designated here mapkks) from various animals and to those of yeast homologs of mapkks. the n-terminal domain of npk2 was produced as a fusion protein in escherichia coli, and the ... | 1995 | 7891653 |
| a large-scale, hospital-based case-control study of risk factors of breast cancer according to menopausal status. | we conducted a large-scale, hospital-based case-control study to evaluate differences and similarities in the risk factors of female breast cancer according to menopausal status. this study is based on a questionnaire survey on life style routinely obtained from outpatients who first visited the aichi cancer center hospital between january 1, 1988 and december 31, 1992. among 36,944 outpatients, 1,186 women with breast cancer detected by histological examination were taken as the case group (607 ... | 1995 | 7730137 |
| acaricidal and insecticidal activities of cadina-4,10 (15)-dien-3-one. | the novel assignment of 13c and 1h nmr data for cadina-4,10(15)-dien-3-one obtained from hyptis verticillata is presented. the study revealed that cadina-4,10(15)-dien-3-one possesses chemosterilant activities against the economically important cattle tick, boophilus microplus, and toxic action against adult cylas formicarius the most destructive pest of sweet potato (i pomoea sp.). | 1995 | 7576456 |
| subunit interaction enhances enzyme activity and stability of sweet potato cytosolic cu/zn-superoxide dismutase purified by a his-tagged recombinant protein method. | the coding region of copper/zinc-superoxide dismutase (cu/zn-sod) cdna from sweet potato, ipomoea batatas (l.) lam. cv. tainong 57, was introduced into an expression vector, pet-20b(+). the cu/zn-sod purified by his-tagged technique showed two active forms (dimer and monomer). the amount of proteins of dimer and monomer appeared to be equal, but the activity of dimeric form was seven times higher than that of monomeric form. the enzyme was dissociated into monomer by imidazole buffer above 1.0 m ... | 1995 | 7599315 |
| fractionation and characterization of two forms of peroxidase from oryza sativa. | peroxidase (e.c. 1.11.1.7., hydrogen donor oxidoreductase) is widely distributed and has been isolated from many higher plants (1). the wide distribution of the enzyme suggests that it could be of great biological importance. however the role that it plays in metabolism is not clear due to the large number of reactions it catalyzes and the considerable number of isozymic species (2). in tomato plants, evans and aldridge (3) separated out six isoperoxidases and in a later paper evans reported 12 ... | 1995 | 7603969 |
| nucleotide sequence analysis of two nuclear inclusion body and coat protein genes of a sweet potato feathery mottle virus severe strain (spfmv-s) genomic rna. | recombinant dna molecules containing cdna of a sweet potato feathery mottle virus severe strain (spfmv-s) rna genome were constructed and the partial nucleotide sequences were determined for three dna inserts, which cover 4-2 kb from the 3'-terminus excluding the poly (a) tail. this region of the genome consists of an open reading frame of 1340 amino acids (a.a.) and a 3'-non-translated region of 224 nucleotides. the protein products expected were 6k2 (53 a.a.), nia (435 a.a.), nib (521 a.a.) an ... | 1995 | 7661699 |
| cloning of the cdna for the human atp synthase oscp subunit (atp5o) by exon trapping and mapping to chromosome 21q22.1-q22.2. | exon trapping was used to clone portions of potential genes from human chromosome 21. one trapped sequence showed striking homology with the bovine and rat atp synthase oscp (oligomycin sensitivity conferring protein) subunit. we subsequently cloned the full-length human atp synthase oscp cdna (gdb/hgmw approved name atp50) from infant brain and muscle libraries and determined its nucleotide and deduced amino acid sequence (embl/genbank accession no. x83218). the encoded polypeptide contains 213 ... | 1995 | 7490082 |
| o-glycosylation of a precursor to a sweet potato vacuolar protein, sporamin, expressed in tobacco cells. | sporamin, a vacuolar protein of the sweet potato, is synthesized as a precursor that contains signal peptide and an n-terminal propeptide that functions as a vacuolar targeting determinant. sporamin, when expressed in tobacco cells, migrated as smeared bands on an sds-polyacrylamide gel. the smearing was due to o-glycosylation of the precursor to sporamin. the smeared bands were stained by a glycan-specific stain but no n-glycosylation site was found in the amino acid sequence of the precursor t ... | 1995 | 8580960 |
| replacement of maize with sweet potato (ipomoea batatas l.) tuber meal in broiler diets. | 1. the nutrient composition of sweet potato tuber meal (spm) was similar to that of maize, with the exceptions of crude protein and calcium. the crude protein content was lower (62 v 91 g/kg) and the calcium content was higher (l.9 v 0.l g/kg) in spm. the amino acid composition, in general, favoured spm. 2. trypsin inhibitor activity, and the contents of oxalate and phytate-phosphorus in spm were too low to be of any nutritional concern. 3. the apparent metabolisable energy content of spm was de ... | 1996 | 8833531 |
| effects of oven-drying tubers of two high-protein sweet potato varieties at different temperatures on their feeding value in broilers. | 1. unpeeled tubers from 2 high-protein varieties of sweet potato (white-fleshed bosbok, and orange-fleshed carmel) were chipped and oven-dried at 40, 60, or 80 degrees c, to examine the effects on nutritive value. the dried ground chips were substituted for maize at 500 g/kg and the diets fed to day-old, ross-1 broiler chicks for 3 weeks. 2. compared with carmel, bosbok had a lower crude protein content (90 v. 138 g/kg dm) and trypsin inhibitor activity (tia) (3 v. 5 mg of trypsin inhibited per ... | 1996 | 8833537 |
| application of sweet potato fiber to skin wound in rat. | in order to introduce a suitable drug mixing base or covering for burns or decubital wounds, the usefulness of fiber extracted from the sweet potato was investigated. the healing effect of the fiber was evaluated by examining the extent of reduction in the size of wounds and changes in the quality of wounds in rats. in contrast to the control, the use of the fiber alone as a wound covering material reduced wounded areas by 21.0% at postoperative day 9, 19.5% at day 11, and 18.7% at day 13, and r ... | 1996 | 8839973 |
| isolation, characterisation and cell growth-regulatory properties of kumara (sweet potato) trypsin inhibitors. | locally grown kumara (sweet potato; ipomea batatis) was used as the starting point for the purification of a proteinase inhibitor. the purified inhibitor was highly specific for trypsin, and much less effective as an inhibitor of chymotrypsin. two 22kda variants were present, closely homologous to each other and to sporamin a, but with a single amino acid substitution (proline in place of serine, the second residue in mature sporamin a). one variant had the same n-terminus as sporamin a, whereas ... | 1996 | 8850529 |
| ultraweak luminescence generated by sweet potato and fusarium oxysporum interactions associated with a defense response. | ultraweak luminescence generated by sweet potato and nonpathogenic fusarium oxysporum interactions associated with a defense response was detected by a photoncounting method using ultrahigh-sensitive photodetectors. the time-dependent intensity variation, the spectrum and the two-dimensional imaging of the ultraweak luminescence are indicative of the defense response of the sweet potato to f. oxysporum. the production of ipomeamarone as a phytoalexin means that f. oxysporum induced the defense r ... | 1996 | 8972637 |
| food production and nutrition in biosphere 2: results from the first mission september 1991 to september 1993. | the initial test of the biosphere 2 agricultural system was to provide a nutritionally adequate diet for eight crew members during a two year closure experiment, 1991-1993. the overall results of that trial are presented in this paper. the 2000 m2 cropping area provided about 80 percent of overall nutritional needs during the two years. adaptation of the crew to the diet which averaged 2200 calories, 73 g. of protein and 32 g. of fat per person over the course of the two years. the diet was ... | 1996 | 11538814 |
| role of nematodes, nematicides, and crop rotation on the productivity and quality of potato, sweet potato, peanut, and grain sorghum. | the objective of this experiment was to determine the effects of fenamiphos 15g and short-cycle potato (po)-sweet potato (sp) grown continuously and in rotation with peanut (pe)-grain sorghum (gs) on yield, crop quality, and mixed nematode population densities of meloidogyne arenaria, m. hapla, m. incognita, and mesocriconema ornatum. greater root-gall indices and damage by m. hapla and m. incognita occurred on potato than other crops. most crop yields were higher and root-gall indices lower fro ... | 1996 | 19277157 |
| interaction of a potential vacuolar targeting receptor with amino- and carboxyl-terminal targeting determinants. | a protein of 80 kd from developing pea (pisum sativum) cotyledons has previously been shown to exhibit characteristics of a vacuolar targeting receptor by means of its affinity for the amino-terminal vacuolar targeting sequence of proaleurain from barley (hordeum vulgare). in this report we show that the same protein also binds to the amino-terminal targeting peptide of prosporamin from sweet potato (ipomoea batatas) and to the carboxyl-terminal targeting determinant of pro-2s albumin from brazi ... | 1996 | 8787025 |
| localized migration and dispersal by the sweet potato whitefly, bemisia tabaci. | laboratory populations of the sweet potato whitefly, bemisia tabaci, have been shown to consist of both migratory and trivial flying morphs. the behavior of these forms as part of the process of short-range migration was examined under field conditions. insects were marked in a field of melons using fluorescent dust during two consecutive growing seasons. during the first growing season, passive traps used to collect living whiteflies, were placed along 16 equally spaced transects radiating from ... | 1996 | 28307104 |
| bombyxin-related peptides: cdna structure and expression in the brain of the hornworm agrius convolvuli [corrected]. | we have cloned three cdnas from the sweet potato hornworm agrius convolvuli that encode precursor molecules for peptides structurally related to bombyxin, an insulin-related brain secretory peptide in bombyx mori. the agrius bombyxin-related peptide (abrp) cdnas are classified into type a and b according to their sequence similarity. the prepro-abrps deduced from the cdna sequences have the insulin-like domain organization of signal peptide/b chain/c peptide/a chain. the abrp transcripts in agri ... | 1996 | 8673077 |
| cryopreservation of non-encapsulated embryogenic tissue of sweet potato (ipomoea batatas). | embryogenic tissue of the sweet potato (ipomoea batatas (l) lam) genotype tib 10 was established from in vitro axillary shoot tips on murashige and skoog (1962) medium supplemented with 5 μm 2,4-dichlorophenoxyacetic acid. embryogenic aggregates of fresh mass 9.0-12 mg were subjected to a rapid freezing protocol in liquid nitrogen following sucrose preculture and varying degrees of dehydration. up to 50% of embryogenic explants survived rapid freezing after preculture on 0.4 or 0.7m sucrose only ... | 1996 | 24178228 |
| specificity of the organic acid activation of alternative oxidase in plant mitochondria. | the claim that succinate and malate can directly stimulate the activity of the alternative oxidase in plant mitochondria (a.m. wagner, c.w.m. van den bergen, h. wincencjusz [1995] plant physiol 108: 1035-1042) was reinvestigated using sweet potato (ipomoea batatas l.) mitochondria. in whole mitochondria, succinate (in the presence of malonate) and both l- and d-malate stimulated respiration via alternative oxidase in a ph- (and nad+)-dependent manner. solubilized malic enzyme catalyzed the oxida ... | 1996 | 12226315 |
| inhibition of mitochondrial respiration by the flavone aglycone isovitexin causes aberrant petal and leaf morphology in silene latifolia. | the morphological mutant "isovitexin" in silene latifolia (the white campion) has small and up-curled petals and leaves. in this mutant the aglycone isovitexin is the only flavone present in the vacuole. in the present study it is shown that isovitexin has a strong toxic effect on mitochondria that is to a large extent abolished by glycosylation. this effect can be used to explain the aberrant morphology. isovitexin acts at the level of the ubiquinone pool; cytochrome c - cytochrome aa3 oxidase ... | 1996 | 24178618 |
| rapid and repetitive plant regeneration in sweetpotato via somatic embryogenesis. | an efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and petiole expiants has been developed in sweetpotato [ipomoea batatas l. (lam.)]. the optimal somatic embryogenic response was obtained in the genotype pi 318846-3 with a two-step protocol: (1) stage i-incubation of expiants in the dark for 2 weeks on murashige skoog (ms) medium containing 2,4-dichlorophenoxyacetic acid (2,4-d) (2.5 mg/l) and 6-benzylaminopurine (0.25 ... | 1996 | 24178413 |