Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
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| protease d from the sarcocarp of honeydew melon fruit. | a serine protease was purified from commercially available honeydew melon fruit (cucumis melo l. var. inodorus naud) by five steps including cm-sepharose cl-6b column chromatography. at the first cm-sepharose cl-6b chromatography four active fractions appeared. no difference in enzymatic properties and m(r) in each fraction was found. finally, honeydew melon protease d was isolated from the major active fraction d. a m(r) of 50,000 was determined for protease d on sds-page and gel filtration. pr ... | 1993 | 7764026 |
| physiological effects of ozone on cultivars of watermelon (citrullus lanatus) and muskmelon (cucumis melo) widely grown in spain. | two cultivars of watermelon (citrullus lanatus) and muskmelon (cucumis melo), which are widely grown in spain, were exposed to ozone (70 nl litre(-1), 6 h d(-1)) for 21 days. ozone sensitivity was assessed by recording the extent of visible injury, changes in fast-fluorescence kinetics, the relative-growth rate (r) of root (rr) and shoot (rs), and effects on the number of flowers produced per plant. leaf gas exchange was measured in order to provide some indication of the factors underlying diff ... | 1993 | 15091805 |
| genetic engineering of potyvirus resistance using constructs derived from the zucchini yellow mosaic virus coat protein gene. | three versions of the zucchini yellow mosaic virus (zymv) coat protein gene were engineered for expression in plants: the full-length coat protein sequence, the conserved core portion of the gene, and an antisense version. these constructs were introduced into muskmelon (cucumis melo) and tobacco plants (nicotiana tabacum) via agrobacterium tumefaciens-mediated transformation; gene expression was verified by northern and western analysis. transgenic r0 and r1 muskmelon plants expressing the full ... | 1993 | 8324251 |
| isolation of a ripening and wound-induced cdna from cucumis melo l. encoding a protein with homology to the ethylene-forming enzyme. | a cdna clone (pmel1) was isolated from a climacteric melon fruit cdna library using the tomato ethylene-forming-enzyme (efe) cdna, ptom13, as a probe. northern analysis of rna isolated from wounded leaf and fruit tissue and from preclimacteric and climacteric pericarp tissue was used to determine the pattern of pmel1 rna expression. pmel1 hybridized to a 1.3-kb transcript in climacteric fruit and wounded leaf tissue but was undetectable in preclimacteric fruit and unwounded leaves. maximal expre ... | 1993 | 8444161 |
| characterization of the formae speciales of fusarium oxysporum causing wilts of cucurbits by dna fingerprinting with nuclear repetitive dna sequences. | the genetic relatedness of five formae speciales of fusarium oxysporum causing wilts of cucurbit plants was determined by dna fingerprinting with the moderately repetitive dna sequences folr1 to folr4. the four folr clones were chosen from a genomic library made from f. oxysporum f. sp. lagenariae 03-05118. total dnas from 50 strains representing five cucurbit-infecting formae speciales, cucumerinum, melonis, lagenariae, niveum, and momordicae, and 6 strains of formae speciales pathogenic to oth ... | 1994 | 8085813 |
| in vitro effect of vegetable and fruit juices on the mutagenicity of 2-amino-3-methylimidazo[4,5-f]quinoline, 2-amino-3,4-dimethylimidazo[4,5-f]quinoline and 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline. | the antimutagenic potencies of the juices of 28 fruits and 34 vegetables commonly consumed in germany were investigated with respect to the mutagenic activities induced by 2-amino-3-methyl[4,5-f]-quinoline (iq), and in part by 2-amino-3,4-dimethylimidazo[4,5-f]quinoline (meiq) or 2-amino-3,8-dimethylimidazo[4,5-f]quinoxaline (meiqx) in salmonella typhimurium ta98 and ta100. with iq, weak to strong antimutagenic activities were found in 68% of the fruits and 73% of the vegetables that were tested ... | 1994 | 8206443 |
| protease from the sarcocarp of trichosanthes bracteata. | a protease has been purified from sarcocarp of trichosanthes bracteata (lam.) voigt by four steps of chromatography. its m(r) was estimated by sds-page to be ca 67,000. the optimum ph of the enzyme was 11 at 35 degrees using casein substrate. the enzyme was strongly inhibited by di-isopropyl fluorophosphate, but not by edta and cysteine protease inhibitors. the oxidized insulin b-chain was cleaved at the peptide bonds of cys7 (so3h)-gly8, glu13-ala14, try16-leu17 by the enzyme for 1 min. the res ... | 1994 | 7764589 |
| cusativin, a new cytidine-specific ribonuclease accumulated in seeds of cucumis sativus l. | dry seeds of cucumis sativus l. were found to contain a heat-sensitive endoribonuclease of a novel type which we have named cusativin. it was purified to apparent electrophoretic homogeneity by chromatography through s-sepharose fast flow, sephadex g-75, cm-sepharose, superdex 75-fplc (fast protein liquid chromatography) and mono s-fplc. it is a single unglycosylated polypeptide chain with an apparent molecular mass (m(r)) of 22900. polyclonal anti-cusativin antibodies raised in rabbits only rea ... | 1994 | 7765423 |
| agrobacterium-mediated transformation of commercial melon (cucumis melo l., cv. amarillo oro). | cotyledon explants of muskmelon (cucumis melo l., cv. amarillo oro) seedlings were co-cultivated with disarmed agrobacterium tumefaciens strain lba4404 that contained the binary vector plasmid pbi121.1. the t-dna region of this binary vector contains the nopaline synthase/neomycin phosphotransferase ii (nptii) chimeric gene for kanamycin resistance and the cauliflower mosaic virus 35s/β-glucuronidase (gus) chimeric gene. after infection, the cotyledon pieces were placed in induction medium conta ... | 1994 | 24193640 |
| cucumisin, a serine protease from melon fruits, shares structural homology with subtilisin and is generated from a large precursor. | cucumisin is a thermostable alkaline serine protease that is found in the juice of melon fruits (cucumis melo l.). we have determined the complete nucleotide sequence of a cucumisin cdna (2,552 nucleotides) and deduced the corresponding amino acid sequence. the open reading frame of the cdna consists of 731 codons and encodes a large precursor (molecular weight, 78,815) relative to the observed size of mature native cucumisin (67 kda). sequence comparisons reveal that cucumisin has several featu ... | 1994 | 7806492 |
| ploidy of somatic embryos and the ability to regenerate plantlets in melon (cucumis melo l.). | the number of chromosomes in cells of callus, somatic embryos and regenerated plantlets during somatic embryogenesis were examined in two cultivars of melon (cucumis melo l.). somatic embryos were diploid (50.0%/32.1%), tetraploid (38.5%/57.5%) and octoploid (11.5%/10.4%) whereas in callus cells diploidy (41.9%/43.3%), tetraploidy (27.9%/25.8%), octoploidy (11.6%/15.5%) and a low frequency of other types of ploidy and aneuploidy were observed. mixoploid somatic embryos were not observed. these r ... | 1994 | 24192875 |
| isoelectric focusing in immobilized ph gradient of melon (cucumis melo l.) seed protein: methodical and genetic aspects, and application in breeding. | genetic variability of melon seed proteins was studied by separation of seed protein by isoelectric focusing in immobilized ph gradient (ief-ipg) under denaturing conditions. a routine procedure was developed for ief-ipg of hundreds of individual melon seeds per day. a group of 74 accessions from 19 morphologically distinct groups and from different geographic origin were studied by ief-ipg using ph gradients of 4-10, 4-7 and 6-10. the electrophoretic analysis of the 74 accessions showed 270 rep ... | 1994 | 7720692 |
| a phenylalanine ammonia-lyase gene from melon fruit: cdna cloning, sequence and expression in response to development and wounding. | phenylalanine ammonia-lyase (pal) is the first enzyme of phenylpropanoid biosynthesis involved in the synthesis of a multiplicity of plant natural products. we have isolated and characterized a nearly full-length cdna clone (pmpal-1) corresponding to a melon fruit (cucumis melo l. var. reticulatus) gene coding for a protein which is highly similar to pal from other plants. melon fruit pal is transcriptionally induced both in response to fruit ripening and wounding. pal gene expression follows th ... | 1994 | 7948894 |
| quantitative competition of calcium with sodium or magnesium for sorption sites on plasma membrane vesicles of melon (cucumis melo l.) root cells. | the presence of ca2+ ions in solution is vital for root growth. the plasma membrane is one of the first sites where competition between ca2+ and other ions occurs. we studied the competition between ca2+ and na+ or mg2+ for sorption sites on the plasma membrane of melon root cells. sorption of 45ca2+ to right-side-out pm vesicles of melon (cucumis melo l.) roots (prepared by aqueous two-phase partitioning) was studied at various ca2+ concentrations, in the presence of increasing concentrations o ... | 1994 | 8189432 |
| improved isolation, stability and substrate specificity of cucumisin, a plant serine endopeptidase. | cucumisin (ec 3.4.21.25), a serine endopeptidase, was isolated by a simple purification procedure from the prince melon (cucumis melo ssp. melo, cv. 'prince melon'). the enzyme is stable over a wide ph range (4-11) and to heat, 80% of its initial activity remaining even at ph 11.1 and at 60 degrees c for 20 min. the enzyme was inactive at 72 degrees c and ph 8.0, but 38% of the activity remained in the presence of 10% (w/v) glycerol. caseinolysis by cucumisin indicated full activity in 8 m urea ... | 1995 | 7576259 |
| selection of somaclonal variants with low-temperature germinability in melon (cucumis melo l.). | plants were regenerated from adventitious buds and somatic embryos (r0) of melon (cucumis melo l.), the cultivar andes. somaclonal variants of melon with low temperature germinability were selected from the progenies (r1) of r0 plants. among 5,618 r1 seeds harvested from 23 r0 plants that were regenerated from adventitious buds 4 seeds germinated after 5 days of culture at 15 °c (selection rate; 0.07%). however, among 374 r2 seeds harvested from 2 r1 plants no seed germinated after 7 days of cul ... | 1995 | 24186622 |
| a new procedure for the preparation of highly active melonin from the dry seeds of cucumis melo l. | melon (cucumis melo l.) dry seeds contain melonin, a protein that strongly inhibits ribosomes from different prokaryotic and eukaryotic sources including those from melon. the protein was purified by a new method to yield highly active and stable protein preparations that involves chromatography through s-sepharose fast flow, cm-sepharose, superdex 75 and mono-s. melonin shows important functional properties: 1) its inhibitory effects on translation were irreversible; 2) it is a single unglycosy ... | 1995 | 7787738 |
| poisoning of cattle attributed to cucumis melo ssp agrestis (ulcardo melon). | 1995 | 8534234 | |
| amino acid sequences of trypsin inhibitors from the melon cucumis melo. | two squash family trypsin inhibitors, cmeti-a and cmeti-b, were isolated from the melon (cucumis melo) seeds, by ion exchange chromatography, gel filtration, affinity chromatography, and high-performance liquid chromatography, and their amino acid sequences were determined. all inhibitors contain 29 amino acid residues including 6 half-cystine residues. they differ by twelve amino acid residues. these polypeptides are strong inhibitors of bovine trypsin, with ki values of 1.6 x 10(-10) m (cmeti- ... | 1995 | 8537309 |
| [isolation of protoplasts from vegetable tissues using extracellular lytic enzymes from fusarium oxysporum f.sp. melonis]. | fusarium oxysporum f.sp. melonis, a pathogen of melon (cucumis melo l.), was grown in shaken cultures at 26 degrees c in a mineral salts medium containing glucose, xylan and apple pectin as carbon sources. the extracellular enzymic complex obtained from these cultures showed lytic activity on plant tissues, causing maceration of melon fruits, potato tubers and carrot roots. protoplasts were isolated from melon fruits when the maceration was carried out under appropriate osmotic conditions. this ... | 1995 | 8850131 |
| a genetic map of melon (cucumis melo l.) with rflp, rapd, isozyme, disease resistance and morphological markers. | one hundred and ten markers were analysed for linkage in 218 f2 plants derived from two divergent cultivars ('védrantais' and 'songwhan charmi') of cucumis melo (l.). thirty-four rflps, 64 rapds, one isozyme, four disease resistance markers and one morphological marker were used to construct a genetic map spanning 14 linkage groups covering 1390 cm of the melon genome. rapd and rflp markers detected similar polymorphism levels. rflps were largely due to base substitutions rather than insertion/d ... | 1996 | 24162199 |
| length polymorphism and homologies of microsatellites in several cucurbitaceae species. | the objectives of this research were to assess (1) the degree of simple sequence repeats (ssr) dna length polymorphism in melon (cucumis melo l.) and other species within the cucurbitaceae family and (2) the possibility of utilizing ssrs flanking primers from single species to other genera or species of cucurbitaceae. five melon (ct/ga) n ssrs were isolated from a genomic library. two cucumber (cucumis sativus l.) ssrs were detected through a search of dna sequence databases, one contained a (ct ... | 1996 | 24162541 |
| structure and expression of three genes encoding acc oxidase homologs from melon (cucumis melo l.). | the enzyme acc oxidase catalyses the last step of ethylene biosynthesis in plants, converting 1-aminocyclopropane-1-carboxylic acid (acc) to ethylene. we have previously described the isolation and characterization of a cdna clone (pmel1) encoding an acc oxidase homolog from melon (cucumis melo l.). here we report the isolation and characterization of three genomic clones, corresponding to three putative members of the acc oxidase gene family in melon. all are transcriptionally active. the seque ... | 1996 | 8628251 |
| beta-carotene content of postharvest orange-fleshed muskmelon fruit: effect of cultivar, growing location and fruit size. | the influence of two growing locations (soil types), six fruit sizes, and two years on the postharvest beta-carotene content of muskmelon (cucumis melo l. var. reticulatus naud.) fruit was studied with two different cultivars. fully abscised commercial size fruit: 9, 12, 15, 18, 23, and 30 (fruit/0.04 m3 shipping box) had highly variable beta-carotene contents (5.3 to 33.8 micrograms/g fresh weight) that varied by size class, soil type and cultivar. beta-carotene content increased with fruit siz ... | 1996 | 8865328 |
| raffinose oligosaccharide concentrations measured in individual cell and tissue types in cucumis melo l. leaves: implications for phloem loading. | raffinose, stachyose, and galactinol are synthesized in intermediary cells (specialized companion cells) of the minor-vein phloem of cucurbits. to better understand the role of these carbohydrates and the regulation of their synthesis and transport, we measured the concentrations of each of the components of the raffinose oligosaccharide synthetic pathway in mesophyll and sieve element-intermediary cell complexes (se-iccs) in the leaves of melon (cucumis melo l. cv. hale's best jumbo). these con ... | 1996 | 28321672 |
| expression of acc oxidase antisense gene inhibits ripening of cantaloupe melon fruits. | the plant hormone ethylene plays a major role in the ripening of climacteric fruit. we have generated transgenic cantaloupe charentais melons expressing an antisense acc oxidase gene; acc oxidase catalyzes the last step of ethylene biosynthesis. ethylene production of transgenic fruit was < 1% of control untransformed fruit, and the ripening process was blocked both on and off the vine. the antisense phenotype could be reversed by exogenous ethylene treatment. analysis of antisense acc oxidase m ... | 1996 | 9631011 |
| substrate specificity of honeydew melon protease d, a plant serine endopeptidase. | the substrate specificity of honeydew melon (cucumis melo var. inodorus naud) protease d was studied by the use of synthetic substrates and oligopeptides derived from a protein hydrolyzate. the hydrolysis rates of succinyl-(l-ala)1-3-p-nitroanilide (suc-(ala)1-3-pna) the hydrolysis rate progressively rose in proportion to the increased chain length. benzyloxycarbonyl-l-tyrosine p-nitrophenyl ester (z-tyr-onp) and benzoyl-l-tyrosine ethyl ester (bz-tyr-oet) were cleaved by honeydew melon protease ... | 1997 | 9301107 |
| ethanol-induced anaphylaxis following ingestion of overripe rock melon, cucumis melo. | anaphylactic reactions to ethanol are rare with only seven cases reported in the literature. all previous cases have occurred following ingestion of alcoholic beverages or food to which an alcoholic beverage has been added. to date there are no cases in the literature of ethanol-induced anaphylaxis caused by the accumulation of endogenous ethanol in overripe fruit. | 1997 | 9087153 |
| purification and some properties of a protease from the sarcocarp of musk melon fruit. | a protease has been purified from sarcocarp of musk melon, cucumis melo ssp. melo var. reticulatus naud. earl's favourite. the protease was mostly present in the placenta part of the fruit and next in the inside mesocarp. the molecular mass of the enzyme was estimated to be about 62kda on sds-page. the enzyme had a carbohydrate moiety. the optimum ph of the enzyme was 11 at 35°c using casein as a substrate. the enzyme was stable between ph 6 and 11. the enzyme was strongly inhibited by diisoprop ... | 1997 | 27396886 |
| characterization of two cdna clones for mrnas expressed during ripening of melon (cucumis melo l.) fruits. | in vitro translation of mrnas and polyacrylamide gel electrophoresis of proteins from melons revealed that several mrnas increased in amount during ripening, indicating the existence of other ripening genes in addition to those cloned previously. to identify ripening-related genes we have screened a ripe melon cdna library and isolated two novel cdna clones (mel2 and mel7) encoding unidentified proteins. southern analysis revealed that mel2 and mel7 are encoded by low-copy-number genes. the mel2 ... | 1997 | 9037149 |
| transfer of the yeast salt tolerance gene hal1 to cucumis melo l. cultivars and in vitro evaluation of salt tolerance. | an agrobacterium-mediated gene transfer method for production of transgenic melon plants has been optimized. the hal1 gene, an halotolerance gene isolated from yeast, was inserted in a chimaeric construct and joined to two marker genes: a selectable-neomycin phosphotransferase-ii (nptii)-, and a reporter-beta-glucuronidase (gus)-. the entire construct was introduced into commercial cultivars of melon. transformants were selected for their ability to grow on media containing kanamycin. transforma ... | 1997 | 9032977 |
| characterization of epitopes on zucchini yellow mosaic potyvirus coat protein permits studies on the interactions between strains. | monoclonal antibodies (mabs) raised against the coat protein of zucchini yellow mosaic potyvirus (zymv) were characterized by epitope mapping using synthetic oligopeptides. two mutant viruses with a mutation in the amino acid sequence important for epitope recognition in vitro were obtained by site-directed mutagenesis of a full-length cdna of zymv. two mabs, cc11 and dd2, could distinguish specifically between these mutants in mixed infections, or after sequential inoculations of muskmelons. se ... | 1997 | 9267009 |
| cloning, expression, and role in pathogenicity of pg1 encoding the major extracellular endopolygalacturonase of the vascular wilt pathogen fusarium oxysporum. | pg1 encoding the major in vitro extracellular endopolygalacturonase of the tomato vascular wilt pathogen fusarium oxysporum f. sp. lycopersici was cloned and sequenced. the deduced mature protein had a calculated molecular mass of 35.5 kda and a pi of 6.2, and showed significant similarity with other fungal endopgs. pg1 mrna was induced in vitro by citrus pectin, tomato vascular tissue, 0.1% d-galacturonic acid, and polygalacturonic acid, and repressed by 1% d-galacturonic acid and 1% glucose. r ... | 1998 | 9450333 |
| endopolygalacturonase pg1 in different formae speciales of fusarium oxysporum | pg1, the major endopolygalacturonase of the vascular wilt pathogen fusarium oxysporum, was secreted during growth on pectin by 10 of 12 isolates belonging to seven formae speciales, as determined with isoelectric focusing zymograms and sodium dodecyl sulfate-polyacrylamide gel electrophoresis gels. a southern analysis of genomic dna and pcr performed with gene-specific primers revealed that the pg1 locus was highly conserved structurally in most isolates. two pg1-deficient isolates were identifi ... | 1998 | 9572983 |
| polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly. | ripening-associated pectin disassembly in melon is characterized by a decrease in molecular mass and an increase in the solubilization of polyuronide, modifications that in other fruit have been attributed to the activity of polygalacturonase (pg). although it has been reported that pg activity is absent during melon fruit ripening, a mechanism for pg-independent pectin disassembly has not been positively identified. here we provide evidence that pectin disassembly in melon (cucumis melo) may be ... | 1998 | 9625689 |
| pathogenic and genetic variation in the japanese strains of fusarium oxysporum f. sp. melonis. | abstract pathogenic variation among 41 japanese strains of fusarium oxysporum f. sp. melonis was analyzed by pathogenicity tests with muskmelon, oriental melon, and oriental pickling melon cultivars. based on pathogenicity to muskmelon cvs. amus and ohi and oriental melon cv. ogon 9, 41 strains were divided into 3 groups that corresponded completely to risser's races 0, 2, and 1,2y. to further characterize pathogenic variation within the forma specialis and races, strains were assayed for pathog ... | 1998 | 18944886 |
| cucumisin like protease from the sarcocarp of benincasa hispida var. ryukyu. | a protease has been purified from the sarcocarp of benincasa hispida (thunb.) cogn. var. ryukyu by two steps of chromatography. its m(r) was estimated by sds-page to be about 67,000. the enzyme was strongly inhibited by diisopropyl fluorophosphate, but not by edta and cysteine protease inhibitors. the substrate having alanine at the position of p1 was the best among the ala-ala-pro-x-pnas (x = ala, lys, phe, glu, and diaminopropionic acid (dap)). the n-terminal sequence of the first 33 residues ... | 1998 | 9887522 |
| callose deposition is responsible for apoplastic semipermeability of the endosperm envelope of muskmelon seeds | semipermeable cell walls or apoplastic "membranes" have been hypothesized to be present in various plant tissues. although often associated with suberized or lignified walls, the wall component that confers osmotic semipermeability is not known. in muskmelon (cucumis melo l.) seeds, a thin, membranous endosperm completely encloses the embryo, creating a semipermeable apoplastic envelope. when dead muskmelon seeds are allowed to imbibe, solutes leaking from the embryo are retained within the enve ... | 1998 | 9733528 |
| temporal sequence of cell wall disassembly in rapidly ripening melon fruit | the charentais variety of melon (cucumis melo cv reticulatus f1 alpha) was observed to undergo very rapid ripening, with the transition from the preripe to overripe stage occurring within 24 to 48 h. during this time, the flesh first softened and then exhibited substantial disintegration, suggesting that charentais may represent a useful model system to examine the temporal sequence of changes in cell wall composition that typically take place in softening fruit. the total amount of pectin in th ... | 1998 | 9625688 |
| characterization of cdnas encoding small and large subunits of adp-glucose pyrophosphorylases from watermelon (citrullus vulgaris s.). | three cdna clones encoding adp-glucose pyrophosphorylases were isolated from a full red fruit cdna library of watermelon (citrullus vulgaris s.). sequence analyses indicated that one clone, wms1, corresponds to the small subunit, and two clones, wml1 and wml2 (a partial gene), are the large subunits of agpase. the presumed agpase proteins encoded by wms1, wml1, and wml2 have 526, 526, and 481 amino acids, respectively. the protein sequences have the conserved amino acids important for the substr ... | 1998 | 9571786 |
| stimulation of benzyladenine-induced in vitro shoot organogenesis and endogenous proline in melon (cucumis melo l.) by fish protein hydrolysates in combination with proline analogues. | a previous study demonstrated that proline is beneficial for improving melon in vitro shoot organogenesis. a natural source of proline and proline precursors can be obtained from fish protein hydrolysates (fph), a byproduct of the fishery industry. proline analogues azetidine-2-carboxylate and hydroxyproline in combination with standardized fph were used to stimulate proline synthesis and benzyladenine-induced shoot organogenesis by exploiting the proposed proline-linked pentose phosphate pathwa ... | 1999 | 10564053 |
| stage- and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon. | we isolated two muskmelon (cucumis melo) cdna homologs of the arabidopsis ethylene receptor genes etr1 and ers1 and designated them cm-etr1 (c. melo etr1; accession no. af054806) and cm-ers1 (c. melo ers1; accession no. af037368), respectively. northern analysis revealed that the level of cm-ers1 mrna in the pericarp increased in parallel with the increase in fruit size and then markedly decreased at the end of enlargement. in fully enlarged fruit the level of cm-ers1 mrna was low in all tissues ... | 1999 | 10318709 |
| a novel alkaline alpha-galactosidase from melon fruit with a substrate preference for raffinose | the cucurbits translocate the galactosyl-sucrose oligosaccharides raffinose and stachyose, therefore, alpha-galactosidase (alpha-d-galactoside galactohydrolase, ec 3.2.1.22) is expected to function as the initial enzyme of photoassimilate catabolism. however, the previously described alkaline alpha-galactosidase is specific for the tetrasaccharide stachyose, leaving raffinose catabolism in these tissues as an enigma. in this paper we report the partial purification and characterization of three ... | 1999 | 10069835 |
| nymphal survival and movement of crawlers of bemisia argentifolii (homoptera: aleyrodidae) on leaf surfaces of selected vegetables. | bemisia argentifolii bellows & perring generally feeds and develops on the under leaf surface of most host plants, but in excess of 50% of the immature population may be found on the upper surface of some host species. this study determined the influence of leaf surface on survival of immature b. argentifolii and the likelihood of movement by the crawler (1st instar) from the upper (adaxial) leaf surface on selected vegetable hosts. laboratory, tests were conducted on 5 vegetables: cantaloupe, c ... | 1999 | 11543187 |
| development and utility of cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflps) linked to the fom-2 fusarium wilt resistance gene in melon (cucumis melo l.). | fusarium wilt, caused by fusarium oxysporum schlecht f. sp. melonis snyder & hans, is a worldwide soil-borne disease of melon (cucumis melo l.). resistance to races 0 and 1 of fusarium wilt is conditioned by the dominant gene fom-2. to facilitate marker-assisted backcrossing with selection for fusarium wilt resistance, we developed cleaved amplified polymorphic sequences (caps) and restriction fragment length polymorphisms (rflp) markers by converting rapd markers e07 (a 1.25-kb band) and g17 (a ... | 1999 | 22665178 |
| evaluation of botulinal toxin production in packaged fresh-cut cantaloupe and honeydew melons. | the ability of clostridium botulinum to produce toxin on cubed, packaged melons was investigated relative to microbial spoilage at various incubation temperatures and in different packaging systems. freshly cut cubes (approximately 2.5 cm3) of cantaloupe and honeydew melons were surface inoculated with a 10 strain mixture of proteolytic and nonproteolytic spores of c. botulinum (10 to 15 cubes per package; approximately 100 total spores per package). to initially evaluate toxin production and sp ... | 1999 | 10456752 |
| a new subtilisin-like proteinase from roots of the dandelion taraxacum officinale webb s. l. | a serine proteinase from roots of taraxacum officinale webb s. l. was isolated by affinity chromatography and gel-filtration on superose 6r using fplc. the enzyme is a 67-kd glycoprotein containing 54% carbohydrate which we have named taraxalisin. the substrate specificity of taraxalisin toward synthetic peptides and oxidized insulin b-chain is comparable with that of cucumisin from cucumis melo and the subtilisin-like serine proteinase macluralisin from maclura pomifera. the proteinase is inact ... | 1999 | 10521720 |
| potassium permanganate staining for differentiation the surface morphology of opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs. | potassium permanganate staining method was developed for differentiation opisthorchis viverrini, haplorchis taichui and phaneropsolus bonnei eggs. the surfaces of o. viverrini, h. taichui and p. bonnei eggs stained permanently and temporarily were similar in appearance even the staining procedures were varied both in concentration and time. determined under light microscope set at 400x, all of these eggs were oval-shaped, operculated at one pole and indistinct small knob at posterior end. o. viv ... | 1999 | 10774711 |
| role of ethylene and abscisic acid in physicochemical modifications during melon ripening. | hormonal metabolism associated with fruit ripening in two cantaloupe muskmelon cultivars, talma and manta, has been studied. the ethylene crisis began on day 33 after fruit set, reaching the maximum values of internal ethylene concentration and ethylene production rate on day 35 after fruit set. this was the optimum moment for consumption as shown by the higher content in soluble solids, ripening index, sensory analysis, and color parameter values. the b parameter and the b/a quotient values in ... | 1999 | 10606610 |
| a hairy root culture of melon produces aroma compounds. | musk melon is the favorite fruit with a high market value in japan, and the fragrance is one of the major factors determining the fruit quality of melon. in this study, mutant melon hairy roots which had been induced by means of the t-dna insertion mutagenesis were found to produce volatile compounds with the fruity fragrance of mature melon. the volatile compounds were extracted and identified by glc-mass spectrometry. some essential oils such as (z)-3-hexenol, (e)-2-hexenal, 1-nonanol, and (z) ... | 2000 | 10775407 |
| cucumber mosaic virus infection affects sugar transport in melon plants. | viral infection often affects carbon assimilation and metabolism in host plants. to better understand the effect of cucumber mosaic virus (cmv) infection on sugar transport, carbohydrate levels and the amounts of the various sugars in the phloem sap were determined in infected melon (cucumis melo l.) plants. source leaves infected with cmv were characterized by high concentrations of reducing sugars and relatively low starch levels. the altered level of carbohydrates was accompanied by increased ... | 2000 | 10859189 |
| inhibitory serpins from wheat grain with reactive centers resembling glutamine-rich repeats of prolamin storage proteins. cloning and characterization of five major molecular forms. | genes encoding proteins of the serpin superfamily are widespread in the plant kingdom, but the properties of very few plant serpins have been studied, and physiological functions have not been elucidated. six distinct serpins have been identified in grains of hexaploid bread wheat (triticum aestivum l.) by partial purification and amino acid sequencing. the reactive centers of all but one of the serpins resemble the glutamine-rich repetitive sequences in prolamin storage proteins of wheat grain. ... | 2000 | 10874043 |
| identification of phloem involved in assimilate loading in leaves by the activity of the galactinol synthase promoter. | the definition of "minor" veins in leaves is arbitrary and of uncertain biological significance. generally, the term refers to the smallest vein classes in the leaf, believed to function in phloem loading. we found that a galactinol synthase promoter, cloned from melon (cucumis melo), directs expression of the gusa gene to the smallest veins of mature arabidopsis and cultivated tobacco (nicotiana tabacum) leaves. this expression pattern is consistent with the role of galactinol synthase in sugar ... | 2000 | 10889241 |
| comparative study on genomes of two japanese melon necrotic spot virus isolates. | nucleotide sequences of the genomes of two japanese melon necrotic spot virus (mnsv) isolates, nh and nk were determined. the open reading frames (orfs) in both genomes encode five proteins: p29 (the pre-readthrough domain of p89), p89 (the readthrough domain of p89 identified as the putative rna-dependent rna polymerase), p14 (the pre-readthrough domain of p7a), p7a (the putative movement protein), and p42 (coat protein, cp). nucleotide and amino acid sequence identities of the five proteins of ... | 2000 | 11332272 |
| cdna probes for detection of specific dsrnas from the fungal pathogen, monosporascus cannonballus. | monosporascus cannonballus is an ascomycete fungus that is the causative agent of monosporascus root rot/vine decline, a serious disease of muskmelon and watermelon. double-stranded rna (dsrna) was identified in approximately 60% of m. cannonballus isolates recovered from infected muskmelon plants in 1993. after repeated laboratory transfer on culture media, the majority of the isolates harboring dsrnas developed degenerate culture phenotypes and showed reduced virulence (hypovirulence) to muskm ... | 2000 | 10680971 |
| characterization of ripening-regulated cdnas and their expression in ethylene-suppressed charentais melon fruit. | charentais melons (cucumis melo cv reticulatus) are climacteric and undergo extremely rapid ripening. sixteen cdnas corresponding to mrnas whose abundance is ripening regulated were isolated to characterize the changes in gene expression that accompany this very rapid ripening process. sequence comparisons indicated that eight of these cdna clones encoded proteins that have been previously characterized, with one corresponding to acc (1-aminocyclopropane-1-carboxylic acid) oxidase, three to prot ... | 2000 | 10712562 |
| cucumisin-like protease from the latex of euphorbia supina. | a protease has been purified from the latex of euphorbia supina rafin by two steps of chromatography. the mr was estimated by sds-page to be 80 kda. its activity was inhibited strongly by diisopropyl fluorophosphate, but not by edta, pepstatin, or cysteine protease inhibitors, indicating that the enzyme is a serine protease. the specificity of the protease is broad, but the preferential cleavage sites were c-terminal sites of hydrophobic amino acid residues. the n-terminal sequence of the first ... | 2000 | 10746875 |
| the relationship of host range, physiology, and genotype to virulence on cantaloupe in pseudomonas syringae from cantaloupe blight epidemics in france. | abstract in 1993, a bacterial blight caused important losses of cantaloupe (cucumis melo var. cantalupensis) in southwestern france and has now been reported in all cantaloupe-growing regions of france. the causal agent of this blight is pseudomonas syringae, although on a worldwide basis this bacterium has not been a major pathogen of melon for over 50 years. to identify the pathovar of the cantaloupe pathogen, we employed biochemical tests, plasmid and chromosomal profiling, and host range stu ... | 2000 | 18944544 |
| simple sequence repeats in cucumis mapping and map merging. | thirty-four polymorphic simple-sequence repeats (ssrs) were evaluated for length polymorphism in melon (cucumis melo l.) and cucumber (cucumis sativus l.). ssr markers were located on three melon maps (18 on the map of 'vedrantais' and pi 161375, 23 on the map of 'piel de sapo' and pi 161375, and 16 on the map of pi 414723 and 'dulce'). in addition, 14 of the markers were located on the cucumber map of gy14 and pi 183967. ssrs proved to be randomly distributed throughout the melon and cucumber g ... | 2000 | 11195350 |
| polyamines and their cellular anti-senescence properties in honey dew muskmelon fruit. | activated oxygen free radicals cause peroxidative damage to all membranes and hasten senescence. polyamines (pas) are effective scavengers of these free radicals produced by lipoxygenase (lox) and phospholipase-d (pl-d). five days prior to abscission (harvest), 'honey brew' (cucumis melo l. (inodorus group)) fruit have a change in the ratio of endogenous spermidine (spd) to putrescine (put), from spd>put to spd<put, which coincides with the onset of fruit senescence. hypodermal-mesocarp tissues ... | 2000 | 11164582 |
| biochemical and microbial changes during the storage of minimally processed cantaloupe. | the effect of storage time on ph, titratable acidity, degrees brix, organic acids, sugars, amino acids, and color of minimally processed cantaloupe melon (cucumis melo l. var. reticulatus naud. cv. mission) was determined at 4 degrees c and 20 degrees c. changes in most of the biochemical parameters with storage time were relatively slow at the lower temperature. at 20 degrees c, a 17% loss in soluble solids and a 2-fold increase in acidity occurred after 2 days. organic acid content also increa ... | 2000 | 11141266 |
| randomized, double-blind, crossover challenge study in 53 subjects reporting adverse reactions to melon (cucumis melo). | few studies have evaluated ige-mediated hypersensitivity to melon with details of clinical reactions confirmed by double-blind, placebo-controlled, food challenges (dbpcfcs). | 2000 | 11080722 |
| the potential of lagenaria rootstock to confer resistance to the carmine spider mite, tetranychus cinnabarinus (acari: tetranychidae) in cucurbitaceae. | antibiosis and resistance of six cucurbita and two lagenaria accessions to the carmine spider mite, tetranychus cinnabarinus boisduval, were evaluated in the laboratory. significant differences among accessions were observed three days after the inoculation of detached leaf discs. the lagenaria accessions, slawi and sus, proved to be the most resistant to mites, with average populations of mite eggs, 87 and 95%, respectively less than that of the susceptible c. pepo accession, orangetti. the cuc ... | 2000 | 10948370 |
| cantaloupe melon peroxidase: characterization and effects of additives on activity. | peroxidase in cantaloupe melon (cucumis melo l. var. reticulatus naud.), a fruit commonly fresh cut processed, was characterized to determine reaction pathway, optimal conditions for activity and effect of some additives on enzymatic action. mn2+, cacl2, nano2 and kinetin had partial inhibitory effects on enzyme activity. activity was effectively inhibited by compounds capable of chelating peroxidase heme iron such as diethyldithiocarbamate and tiron, but unaffected by edta. free radical scaveng ... | 2000 | 10907237 |
| purification and kinetic characterization of an anionic peroxidase from melon (cucumis melo l.) cultivated under different salinity conditions. | the partial characterization of an anionic peroxidase in melon fruit is described. four melon peroxidase (mpx) isoenzymes were detected in crude extracts after isoelectric focusing. the major mpx isoenzyme (pi = 3.7) was partially purified by including hydrophobic and anion-exchange chromatography in the purification scheme. the sample obtained was used to characterize mpx. this peroxidase did not show activity on ascorbic acid but oxidized guaiacol at a high rate, showing an optimum ph of 5.5 w ... | 2000 | 10820055 |
| volatile components in aqueous essence and fresh fruit of cucumis melo cv. athena (muskmelon) by gc-ms and gc-o. | a comparative study between the aromatic profile of muskmelon aqueous essence and the puree of fresh fruit was carried out using gas chromatography-mass spectrometry (gc-ms) and gas chromatography-olfactometry (gc-o). results obtained show a total of 53 components quantified in the essence and 38 in the fresh fruit. in addition, four new components are described for the first time as contributors to the aromatic profile of muskmelon including 2-methyl-3-buten-2-ol, 2,3-butanediol, methyl 3-pheny ... | 2001 | 11743787 |
| construction of a reference linkage map for melon. | a map of melon (cucumis melo l.) with 411 markers (234 rflps, 94 aflps, 47 rapds, 29 ssrs, five inter-ssrs, and two isozymes) and one morphological trait (carpel number) was constructed using the f2 progeny of a cross between the korean accession p1161375 and the spanish melon type 'pinyonet piel de sapo'. rflps were obtained using 212 probes from different genomic and cdna melon libraries, including 16 arabidopsis ests, 13 cucumis known genes, and three resistant gene homologues. most loci (391 ... | 2001 | 11681608 |
| resistance to diaphania hyalinata (lepidoptera: crambidae) in cucumis species. | diaphania hyalinata (l.), the melonworm, causes economic damage in cucurbit species in the caribbean region. to control larvae feeding on leaves, frequent pesticide applications are needed, but this favors outbreaks of other pests. we looked for plant resistance to d. hyalinata in cucumis melo l. and two wild cucumis, c. metuliferus meyer and c. pustulatus naudin. fifteen genotypes were observed using a 1-81 damage scale during 8 wk in two field trials in guadeloupe, french west indies. among th ... | 2001 | 11425029 |
| acetyl-coa: alcohol acetyltransferase activity and aroma formation in ripening melon fruits. | melon varieties (cucumis melo l.) differ in a range of physical and chemical attributes. sweetness and aroma are two of the most important factors in fruit quality and consumer preference. volatile acetates are major components of the headspace of ripening cv. arava fruits, a commercially important climacteric melon. in contrast, volatile aldehydes and alcohols are most abundant in cv. rochet fruits, a nonclimacteric melon. the formation of volatile acetates is catalyzed by alcohol acetyltransfe ... | 2001 | 11262031 |
| biogenesis of volatile aldehydes from fatty acid hydroperoxides: molecular cloning of a hydroperoxide lyase (cyp74c) with specificity for both the 9- and 13-hydroperoxides of linoleic and linolenic acids. | a novel member of the plant cytochrome p450 cyp74 family of fatty acid hydroperoxide metabolizing enzymes has been cloned from melon fruit (cucumis melo). the cdna is comprised of 1,446 nucleotides encoding a protein of 481 amino acids. the homology at the amino acid level to other members of the cyp74 family is 35-50%, the closest relatives being allene oxide synthases. the cdna was expressed in escherichia coli, and the corresponding protein was purified by affinity column chromatography. the ... | 2001 | 11368353 |
| cloning and characterization of the gene encoding 3-hydroxy-3-methylglutaryl coenzyme a reductase in melon (cucumis melo l. reticulatus). | we have isolated a cdna for cm-hmgr, encoding 3-hydroxy-3-methylglutaryl coenzyme a (hmg-coa) reductase in melon (cucumis melo l. reticulatus; genbank accession no. ab021862). cm-hmgr encodes a polypeptide of 588 amino acids that contains two transmembrane domains and a catalytic domain. database searches revealed that cm-hmgr shows homology to hmg1 (63.7%) and hmg2 (70.3%) of tomato, to hmg1 (77.2%) and hmg2 (69.4%) of arabidopsis thaliana, and to hmgr of tobacco (72.6%). functional expression ... | 2001 | 11370859 |
| examination of bacteriophage as a biocontrol method for salmonella on fresh-cut fruit: a model study. | the preparation and distribution of fresh-cut produce is a rapidly developing industry that provides the consumer with convenient and nutritious food. however, fresh-cut fruits and vegetables may represent an increased food safety concern because of the absence or damage of peel and rind, which normally help reduce colonization of uncut produce with pathogenic bacteria. in this study, we found that salmonella enteritidis populations can (i) survive on fresh-cut melons and apples stored at 5 degr ... | 2001 | 11510645 |
| salicylic acid and ethylene pathways are differentially activated in melon cotyledons by active or heat-denatured cellulase from trichoderma longibrachiatum. | infiltration of cellulase (ec 3.2.1.4) from trichoderma longibrachiatum into melon (cucumis melo) cotyledons induced several key defense mechanisms and hypersensitive reaction-like symptoms. an oxidative burst was observed 3 hours after treatment and was followed by activation of ethylene and salicylic acid (sa) signaling pathways leading to marked induction of peroxidase and chitinase activities. the treatment of cotyledons by heat-denatured cellulase also led to some induction of peroxidase an ... | 2001 | 11553761 |
| [microbiological stability study of minimally processed cantaloupe (cucumis melo l) by vacuum]. | in order to design a minimal process for cantaloupe, the water activity of the fruit was reduced until 0.976 through vacuum osmotic dehydration and the optimum combination of microbial preservatives (100 ppm sodium sulfite, 600 ppm potassium sorbate, 0.5% l-ascorbic acid and 1% citric acid), that contributed to minimize (p < 0.05), the content of aerobic mesophilic bacteria, molds and yeast, was determined through a 2k multifactorial design. in the second experimental stage, the conditions previ ... | 2001 | 11678049 |
| [host plants of aphis gossypii (aphididae), vector of virus of cucumis melo melon (cucurbitaceae) in costa rica]. | plant species associated with commercial melon crops and surrounding areas were examined to identity the natural host plants of aphis gossypii glover. the study was conducted in two farms located in different melon production areas and plant life zones of costa rica. plant species diversity, percent coverage and distribution over time were recorded during one year. differences between locations were observed. a total of 86 plant species (49 families) and 72 plant species (40 families) were ident ... | 2001 | 11795159 |
| identification of volatile compounds in cantaloupe at various developmental stages using solid phase microextraction. | using an automated rapid headspace solid phase microextraction (spme) method for volatile extraction in cantaloupes, 86 compounds already reported for muskmelons were recovered and an additional 53 compounds not previously reported were identified or tentatively identified. the spme method extracted a copious number of volatiles that can be analyzed to clearly differentiate between variety, growth stage, and stage of harvest ripeness. most of the newly reported compounds in cantaloupe were ester ... | 2001 | 11312862 |
| effect of storage on some volatile aroma compounds in fresh-cut cantaloupe melon. | changes in volatile aroma constituents of fresh-cut cantaloupe melon with storage were determined by headspace solid-phase microextraction gas chromatography-mass spectrometry. the compounds isolated from the fruit immediately after cutting were predominantly aliphatic and aromatic esters. storage of fruit at 4 degrees c caused a considerable decrease in concentration of esters and synthesis of the terpenoid compounds beta-ionone and geranylacetone over a period of 24 h. this change in the volat ... | 2002 | 12083880 |
| role of ethylene in the biosynthetic pathway of aliphatic ester aroma volatiles in charentais cantaloupe melons. | compared to other melon types, cantaloupe charentais melons are highly aromatic with a major contribution to the aroma being made by aliphatic and branched esters. using a transgenic line in which the synthesis of the plant hormone ethylene has been considerably lowered by antisense acc oxidase mrna (as), the aliphatic ester pathway steps at which ethylene exerts its regulatory role were found. the data show that the production of aliphatic esters such as hexyl and butyl acetate was blocked in a ... | 2002 | 11807123 |
| molecular and genetic characterization of a non-climacteric phenotype in melon reveals two loci conferring altered ethylene response in fruit. | fruit ripening and abscission are associated with an ethylene burst in several melon (cucumis melo) genotypes. in cantaloupe as in other climacteric fruit, exogenous ethylene can prematurely induce abscission, ethylene production, and ripening. melon genotypes without fruit abscission or without ethylene burst also exist and are, therefore, non-climacteric. in the nonabscising melon fruit pi 161375, exogenous ethylene failed to stimulate abscission, loss of firmness, ethylene production, and exp ... | 2002 | 12011360 |
| isolation and identification of a phosphate deficiency-induced c-glycosylflavonoid that stimulates arbuscular mycorrhiza formation in melon roots. | melon (cucumis melo) roots were inoculated with or without the arbuscular mycorrhizal (am) fungus glomus caledonium under low phosphate conditions. high-performance liquid chromatography analysis of the secondary metabolites in butanol extracts from roots revealed that the level of one compound in noninoculated roots showed a significant increase from 30 days postinoculation. no accumulation was observed in mycorrhizal roots and high-phosphate-supplemented roots, indicating that the accumulation ... | 2002 | 12026171 |
| relationship of cell surface charge and hydrophobicity to strength of attachment of bacteria to cantaloupe rind. | the cantaloupe melon has been associated with outbreaks of salmonella infections. it is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. the surface charge and hydrophobicity of strains of salmonella, escherichia coli (o157:h7 and non-o157:h7), and listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. initial bacterial attachment to c ... | 2002 | 12117240 |
| sphingomonas melonis sp. nov., a novel pathogen that causes brown spots on yellow spanish melon fruits. | a polyphasic taxonomic study was performed on the phytopathogenic bacterial strains dapp-pg 224(t) and dapp-pg 228, which cause brown spot on yellow spanish melon (cucumis melo var. inodorus) fruits. based on the presence of glucuronosyl ceramide (sgl-1) in cellular lipids, the results of fatty acid analysis and 16s rdna sequence comparison, the strains had been identified as belonging to the genus sphingomonas and as phylogenetically related to sphingomonas mali, sphingomonas pruni and sphingom ... | 2002 | 12508872 |
| resistance gene homologues in melon are linked to genetic loci conferring disease and pest resistance. | genomic and cdna fragments with homology to known disease resistance genes (rgh fragments) were cloned from cucumis melo using degenerate-primer pcr. fifteen homologues of the nbs-lrr gene family have been isolated. the nbs-lrr homologues show high divergence and, based on the partial nbs-fragment sequences, appear to include members of the two major subfamilies that have been described in dicot plants, one that possesses a tir-protein element and one that lacks such a domain. genomic organizati ... | 2002 | 12582612 |
| internal transcribed spacer regions 1 and 2 and random amplified polymorphic dna analysis of didymella bryoniae and related phoma species isolated from cucurbits. | abstract didymella bryoniae (anamorph phoma cucurbitacearum) is the causal agent of gummy stem blight, although other phoma species are often isolated from cucurbit plants exhibiting symptoms of the disease. the molecular and phylogenetic relationships between d. bryoniae and these phoma species are unknown. isolates of d. bryoniae and phoma obtained from cucurbits grown at various geographical locations in the united states were subjected to random amplified polymorphic dna (rapd) analysis and ... | 2002 | 18944025 |
| isolation of nonpathogenic mutants of fusarium oxysporum f. sp. melonis for biological control of fusarium wilt in cucurbits. | abstract two nonpathogenic mutant strains 4/4 and 15/15 of fusarium oxysporum f. sp. melonis (race 1,2) were isolated by a continuous dipinoculation technique following uv mutagenesis of the virulent wild-type isolate fom1.2. no disease symptoms or detrimental effects were observed following inoculation of muskmelon seedlings by strain 4/4. in contrast, strain 15/15 caused mortality of susceptible cultivars although to a lesser extent than the wild-type isolate. strain 4/4 colonized a variety of ... | 2002 | 18943089 |
| relationship between crop losses and initial population densities of meloidogyne arenaria in winter-grown oriental melon in korea. | to determine the economic threshold level, oriental melon (cucumis melo l. cv. geumssaragi-euncheon) grafted on shintozoa (cucurbita maxima x cu. moschata) was planted in plots (2 x 3 m) under a plastic film in february with a range of initial population densities (pi) of meloidogyne arenaria. the relationships of early, late, and total yield to pi measured in september and january were adequately described by both linear regression and the seinhorst damage model. initial nematode densities in s ... | 2002 | 19265907 |
| carvacrol and cinnamic acid inhibit microbial growth in fresh-cut melon and kiwifruit at 4 degrees and 8 degrees c. | to establish whether or not carvacrol and cinnamic acid delay microbial spoilage of fresh-cut fruit. | 2002 | 12390487 |
| detection of 3-hydroxy-3-methylglutaryl-coenzyme a reductase protein cm-hmgr during fruit development in melon ( cucumis melo l.). | the fruit size of melon ( cucumis melo l. reticulatus) is determined by the amount of cell proliferation in the pericarp during early fruit development. during this stage, expression and activity of the 3-hydroxy-3-methylglutaryl coenzyme a reductase (hmgr) gene is required for fruit growth. in this study, we performed a detailed analysis of the correlation between the expression of melon hmgr (cm-hmgr) protein and cell division in the pericarp. flow cytometric analysis revealed that the length ... | 2002 | 12582637 |
| a reference map of cucumis melo based on two recombinant inbred line populations. | a composite genetic melon map was generated based on two recombinant inbred line (ri) populations. by analyzing the segregation of 346 aflps, 113 imas and phenotypic characters on a ri population of 163 individuals derived from the cross védrantais x pi 161375, a first map was constructed. about 20% of the molecular markers were skewed, and the residual heterozygosity was estimated at 4.43% which was not significantly different from the theoretical value of 4.2%. the genome distribution of molec ... | 2002 | 12582608 |
| genetics of ethylene biosynthesis and restriction fragment length polymorphisms (rflps) of acc oxidase and synthase genes in melon ( cucumis melo l.). | we investigated the genetics of ethylene biosynthesis and its linkage to the rflps of the acc oxidase and synthase genes in melon ( cucumis melo l.). the results suggested that the a(0) and b(0) fragments of rflp-mel1 of the acc oxidase gene were two alleles from a single locus, as were the b and c fragments of rflp-meacs1 of the acc synthase gene. the b(0) allele seemed to be partially dominant over the a(0) allele, whereas b and c alleles appeared to map to quantitative trait loci (qtls), whic ... | 2002 | 12582544 |
| characterization of an abscisic acid responsive gene homologue from cucumis melo. | a cdna and genomic dna encoding an abscisic acid responsive gene (asr) homologue (asr1) was isolated from an inodorus melon, cucumis melo var. kuwata, cdna and genomic library. the asr1 gene showed the strongest fruit-specific expression and differential expression profiles during fruit development, which were expressed from a low copy gene. the promoter region of the asr1 gene contained several putative functional cis-elements, which may be involved in the response to plant hormones and environ ... | 2002 | 12379794 |
| cloning of disease-resistance homologues in end sequences of bac clones linked to fom-2, a gene conferring resistance to fusarium wilt in melon (cucumis melo l.). | disease resistance has not yet been characterized at the molecular level in cucurbits, a group of high-value, nutritious, horticultural plants. previously, we genetically mapped the fom-2 gene that confers resistance to fusarium wilt races 0 and i of melon. in this paper, two cosegregating codominant markers (am, aflp marker; fm, fusarium marker) were used to screen a melon bacterial artificial chromosome (bac) library. identified clones were fingerprinted and end sequenced. fingerprinting analy ... | 2002 | 12033615 |
| ultraviolet induced stress response in fresh cut cantaloupe. | changes in the composition of volatile compounds in cantaloupe melon (cucumis melo l. var. reticulatus) as a result of uv induced stress were determined by gas chromatography-mass spectrometry (gc-ms). several volatile ester compounds were present, of which twenty-seven were identified in fresh cut cantaloupe. fruit exposure to uv light decreased the concentrations of most of the aliphatic esters by over 60% of the amounts present in the corresponding fresh cut fruit. cyclic and acyclic terpenoi ... | 2002 | 11985848 |
| genetic control of fruit shape acts prior to anthesis in melon ( cucumis melo l.). | genetic control of fruit shape in cucumis melo was studied using qtl analysis in two recombinant inbred (ri) populations consisting of 163 and 63 individuals, respectively, obtained by crossing the same round-fruited parent with two different elongated-fruit lines. fruit shape is mainly explained by fruit length in these two populations. most qtls for fruit shape and ovary shape detected were found to co-segregate, thus demonstrating early control of fruit shape during ovary development. a high ... | 2002 | 11862487 |
| detection of ethylene receptor protein cm-ers1 during fruit development in melon (cucumis melo l.). | antibodies against melon ethylene receptor, cm- ers1 was prepared. cm-ers1 protein formed a disulphide-linked homodimer and it was present in microsomal membranes but not in soluble fractions. cm-ers1 protein was present at high levels in melon fruit during early developmental stages. this transition pattern was also observed in another melon cultivar. | 2002 | 11847239 |
| tgtcaca motif is a novel cis-regulatory enhancer element involved in fruit-specific expression of the cucumisin gene. | cucumisin, a subtilisin-like serine protease, is expressed at high levels in the fruit of melon (cucumis melo l.) and accumulates in the juice. we investigated roles of the promoter regions and dna-protein interactions in fruit-specific expression of the cucumisin gene. in transient expression analysis, a chimeric gene construct containing a 1.2-kb cucumisin promoter fused to a beta-glucuronidase (gus) reporter gene was expressed in fruit tissues at high levels, but the promoter activities in le ... | 2002 | 11782472 |
| linkage map of cucumis melo including phenotypic traits and sequence-characterized genes. | a new linkage map of cucumis melo, derived from the f2 progeny of a cross between pi 414723 and c. melo 'topmark' is presented. the map spans a total of 1421 cm and includes 179 points consisting of random amplified polymorphic dna (rapd), amplified fragment length polymorphism (aflp), inter-simple sequence repeats (issrs), simple sequence repeats (ssrs), and restriction fragment length polymorphism (rflp) markers. the map also includes an aphid resistance trait (vat) and the sex type gene, andr ... | 2003 | 14608393 |
| clinical characteristics of melon (cucumis melo) allergy. | although melon is a frequent allergy-eliciting fruit, allergic reactions to melon have rarely been reported. | 2003 | 14533664 |
| genetic analysis of spanish melon ( cucumis melo l.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions. | genetic relationships among 125 spanish melon ( cucumis melo l.) accessions from a spanish germplasm collection were assessed using a standard molecular-marker array consisting of 34 random amplified polymorphic dna (rapd) markers bands (19 primers) and 72 reference accessions drawn from previous studies. the reference accession array consisted of a broad range [japanese (19) crete (17), african (15), and usa and europe (us/eu, 21)] of horticultural groupings (group cantalupensis, group conomon, ... | 2003 | 14504742 |
| cloning and functional expression of alkaline alpha-galactosidase from melon fruit: similarity to plant sip proteins uncovers a novel family of plant glycosyl hydrolases. | raffinose and stachyose are ubiquitous galactosyl-sucrose oligosaccharides in the plant kingdom which play major roles, second only to sucrose, in photoassimilate translocation and seed carbohydrate storage. these sugars are initially metabolised by alpha-galactosidases (alpha-gal). we report the cloning and functional expression of the first genes, cmaga1 and cmaga2, encoding for plant alpha-gals with alkaline ph optima from melon fruit (cucumis melo l.), a raffinose and stachyose translocating ... | 2003 | 12943544 |