Publications

molecular dissection of interspecific variation between gossypium hirsutum and gossypium barbadense (cotton) by a backcross-self approach: i. fiber elongation.	the current study is the first installment of an effort to explore the secondary gene pool for the enhancement of upland cotton (gossypium hirsutum l.) germplasm. we developed advanced-generation backcross populations by first crossing g. hirsutum cv. tamcot 2111 and g. barbadense cv. pima s6, then independently backcrossing f(1) plants to the g. hirsutum parent for three cycles. genome-wide mapping revealed introgressed alleles at an average of 7.3% of loci in each bc(3)f(1) plant, collectively ...	2005	15983756
genetic diversity and geographic pattern in early south american cotton domestication.	amplified fragment length polymorphism fingerprinting was applied to survey the genetic diversity of primitive south american gossypium barbadense cotton for establishing a possible link to its pre-columbian expansion. new germplasm was collected along coastal peru and over an andean transect in areas where most of the archaeological evidence relating to cotton domestication has been recorded. gene bank material of three diploid (g. raimondii, g. arboreum, and g. herbaceum) and four allotetraplo ...	2005	15580473
circadian mating activity and effect of pheromone pre-exposure on pheromone response rhythms in the moth spodoptera littoralis.	mating in moths is generally mediated by female-produced sex pheromones. mating activity, female pheromone production/release and male pheromone responsiveness all show diurnal variations in many species. we found that the response of the male egyptian cotton leafworm, spodoptera littoralis, to sex pheromone gland extracts showed a diel rhythm in olfactometer tests, and the variation was persistent for at least 1 day in constant darkness. high male response to sex pheromone was correlated in tim ...	2005	15749110
[identification and characterization of differentially expressed ests of gossypium barbadense infected by verticillium dahliae with suppression subtractive hybridization].	cotton wilt defense reaction is a complicated continuous process and involves a battery of genes. in this study, we adopted suppression subtractive hybridization (ssh) technique to isolate differentially expressed ests from gossypium barbadense variety 7124 during verticillium wilt defense process. an array of 1165 clones from the subtractive library has been screened with reverse northern blotting, of which 131 ests were considered as over-expressed and 16 ests were down-regulated. sequence ana ...	2005	15856944
[construction and analysis of ssh library of gossypium barbadense upon infection with verticillium dahliae].	roots were collected from the seedlings inoculated with pathogen verticillium dahliae after 2, 4, 8, 12, 24, 48, 72 and 96 hours for total rna extraction. the cdnas from the inoculated seedlings were used as the tester and those from the control seedlings as the driver. ssh method was employed to find the differently expressed cdnas responding to the pathogen. t/a clone library was constructed containing 534 clones. the cdna inserts were amplified from the bacterial clones directly with m13 prim ...	2005	16018265
establishment and characterization of spodoptera littoralis cell line (sl 96), adapted at 19 degrees c permissive for different baculoviruses.	cells from ovarioule tissues of the egyptian cotton leaf worm, spodoptera littoralis (sl 96) were established, identified and characterized comparing with other lepidopteran cell lines using isozymes, karyotyping, rapd pcr, and polyacrylamide gel electrophoresis polypeptide page profile. comparison of the number of chromosomes and page profiles; also the isozymes profiles demonstrate the possibility to distinguish the cell lines by those analyses and having a distinguishable finger print for eve ...	2005	16333885
a novel function for the cathepsin d inhibitor in tomato.	proteinaceous aspartic proteinase inhibitors are rare in nature and are described in only a few plant species. one of them corresponds to a family of cathepsin d inhibitors (cdis) described in potato (solanum tuberosum), involving up to 15 isoforms with a high sequence similarity. in this work, we describe a tomato (solanum lycopersicum) wound-inducible protein called jasmonic-induced protein 21 (jip21). sequence analysis of its cdna predicted a putative function as a cdi. the jip21 gene, whose ...	2006	17012408
wide-cross whole-genome radiation hybrid mapping of the cotton (gossypium barbadense l.) genome.	whole-genome radiation hybrid mapping has been applied extensively to human and certain animal species, but little to plants. we recently demonstrated an alternative mapping approach in cotton (gossypium hirsutum l.), based on segmentation by 5-krad gamma-irradiation and derivation of wide-cross whole-genome radiation hybrids (wwrhs). however, limitations observed at the 5-krad level suggested that higher doses might be advantageous. here, we describe the development of an improved second-genera ...	2006	16362372
a fusarium wilt resistance gene in gossypium barbadense and its effect on root-knot nematode-wilt disease complex.	abstract fusarium wilt, caused by the soilborne pathogen fusarium oxysporum f. sp. vasinfectum race 1, is a vascular disease in cotton (gossypium spp.), and is a component of a disease complex with root-knot nematodes (meloidogyne incognita). genetic analysis of two interspecific crosses (g. barbadense pima s-7 x g. hirsutum acala nemx and pima s-7 x acala sj-2) showed that one major gene (designated fov1) with allele dosage effect conferred resistance to f. oxysporum f. sp. vasinfectum race 1 i ...	2006	18943146
isolation and characterization of resistance and defense gene analogs in cotton (gossypium barbadense l.).	plant disease resistance gene (r gene) and defense response gene encode some conserved motifs. in the present work, a pcr strategy was used to clone resistance gene analogs (rgas) and defense gene analogs (dgas) from sea-island cotton variety hai7124 using oligonucleotide primers based on the nucleotide-binding site (nbs) and serine/threonine kinase (stk) in the r-gene and pathogenesis-related proteins of class 2 (pr2) of defense response gene. 79 nbs sequences, 21 stk sequences and 11 dgas were ...	2006	17312991
qtl mapping for resistance to root-knot nematodes in the m-120 rnr upland cotton line (gossypium hirsutum l.) of the auburn 623 rnr source.	root-knot nematodes meloidogyne incognita (kofoid and white) can cause severe yield loss in cotton (gossypium hirsutum l.). the objectives of this study were to determine the inheritance and genomic location of genes conferring root-knot nematode resistance in m-120 rnr, a highly resistant g. hirsutum line with the auburn 623 rnr source of resistance. utilizing two interspecific f(2) populations developed from the same m-120 rnr by gossypium barbadense (cv. pima s-6) cross, genome-wide scanning ...	2006	16960714
isolation and characterization of a class iii homeodomain-leucine zipper-like gene from gossypium barbadense.	class iii homeodomain-leucine zipper (hd-zip iii) genes are important plant-specific transcription factors which have key roles in different stages of vascular and interfascicular fiber differentiation. a novel hd-zip iii gene, designated gbhb1, was isolated by suppression subtraction hybridization and race (rapid amplification of cdna ends) from gossypium barbadense (sea-island cotton). the gbhb1 cdna has a total length of 3061 bp with an open reading frame of 2508 bp, encoding a predicated pol ...	2006	17343206
complete nucleotide sequence of the cotton (gossypium barbadense l.) chloroplast genome with a comparative analysis of sequences among 9 dicot plants.	recently, the complete chloroplast genome sequences of many important crop plants were determined, and this can be considered a major step forward toward exploiting the usefulness of chloroplast genetic engineering technology. economically, cotton is one of the most important crop plants for many countries. to further our understanding of this important crop, we determined the complete nucleotide sequence of the chloroplast genome from cotton (gossypium barbadense l.). the chloroplast genome of ...	2006	17159292
construction and characterization of the first bacterial artificial chromosome library for the cotton species gossypium barbadense l.	as the second most widely cultivated cotton, gossypium barbadense is well known for its superior fiber properties and its high levels of resistance to fusarium and verticillium wilts. to enhance our ability to exploit these properties in breeding programs, we constructed the first bacterial artificial chromosome (bac) library for this species. the library contains 167 424 clones (49 920 bamhi and 117 504 hindiii clones), with an estimated average insert size of 130 kb. about 94.0% of the clones ...	2006	17426754
isolation of 6-methoxy gossypol and 6,6'-dimethoxy gossypol from gossypium barbadense sea island cotton.	6-methoxy gossypol and 6,6'-dimethoxy gossypol were isolated from the seeds and root bark of a st. vincent sea island variety of cotton (azk-267, grin# pi 528406). crude mixtures of gossypol and the methoxy compounds were obtained by extraction of the tissue with acetone and precipitation with acetic acid. after recrystallization, the preparations were treated with 3-amino-1-propanol to form gossypol schiff's bases, which were separated by preparative reverse phase chromatography. the separated ...	2006	16637683
an additive-dominance model to determine chromosomal effects in chromosome substitution lines and other gemplasms.	when using chromosome substitution (cs) lines in a crop breeding improvement program, one needs to separate the effects of the substituted chromosome from the remaining chromosomes. this cannot be done with the traditional additive-dominance (ad) model where cs lines, recurrent parent, and their hybrids are used. in this study, we develop a new genetic model and software, called a modified ad model with genotype x environment interactions, which can predict additive and dominance genetic effects ...	2006	16341682
genetic analysis for f1 yield traits with conditional approach in island cotton (gossypium barbadense l.).	a genetic model with additive, dominance and genotype x environment interaction effect was employed to analyze the 3-year data of f(1) hybrids from 5 x 4 diallel cross, whose parents were island cotton and had different fruit branch types. unconditional and conditional genetic variances were conducted for analyze genetic impacts of yield components on yield. results of unconditional genetic variances showed that there were no additive variance of total lint yield. but conditional additive effect ...	2006	16980131
genes expression analyses of sea-island cotton (gossypium barbadense l.) during fiber development.	sea-island cotton (gossypium barbadense l.) is one of the most valuable cotton species due to its silkiness, luster, long staples, and high strength, but its fiber development mechanism has not been surveyed comprehensively. we constructed a normalized fiber cdna library (from -2 to 25 dpa) of g. barbadense cv. pima 3-79 (the genetic standard line) by saturation hybridization with genomic dna. we screened pima 3-79 fiber rna from five developmental stages using a cdna array including 9,126 plasm ...	2007	17377794
effects of potato plants expressing a barley cystatin on the predatory bug podisus maculiventris via herbivorous prey feeding on the plant.	the aim of this study was to assess the effects of potato plants expressing a barley cystatin on a potentially cystatin-susceptible natural enemy by predation on susceptible and non-susceptible preys feeding on the plant. we have focussed on the impact of the variant hvcpi-1 c68 --> g, in which the only cysteine residue was changed by a glycine, on the growth and digestive physiology of the colorado potato beetle (cpb), leptinotarsa decemlineata, and the egyptian cotton leafworm (ecw), spodopter ...	2007	17072562
biological performance of quercetin on the cotton leaf-worm larvae, spodoptera littoralis boisd. (lep., noctuidae) and prevailing natural enemies in the egyptian cotton fields.	the egyptian cotton (gossypium barbadense l.) accounts for 65 % of the world production of long stable cultivars. taking into consideration the competition of other cotton producing countries, it should be of great importance to control pests, which attack the cotton plants to improve the yield and its quality. the main objective of this study is to develop new approaches for the management of the cotton leafworm spodoptera littoralis boisd. within an ipm program, that include synthetic insectic ...	2007	18399494
preceramic adoption of peanut, squash, and cotton in northern peru.	the early development of agriculture in the new world has been assumed to involve early farming in settlements in the andes, but the record has been sparse. peanut (arachis sp.), squash (cucurbita moschata), and cotton (gossypium barbadense) macrofossils were excavated from archaeological sites on the western slopes of the northern peruvian andes. direct radiocarbon dating indicated that these plants grew between 9240 and 5500 (14)c years before the present. these and other plants were recovered ...	2007	17600214
a potato carboxypeptidase inhibitor gene provides pathogen resistance in transgenic rice.	a defensive role against insect attack has been traditionally attributed to plant protease inhibitors. here, evidence is described of the potential of a plant protease inhibitor, the potato carboxypeptidase inhibitor (pci), to provide resistance to fungal pathogens when expressed in rice as a heterologous protein. it is shown that rice plants constitutively expressing the pci gene exhibit resistance against the economically important pathogens magnaporthe oryzae and fusarium verticillioides. a m ...	2007	17547659
over-expression gberf2 transcription factor in tobacco enhances brown spots disease resistance by activating expression of downstream genes.	erf transcription factors can bind gcc boxes or non-gcc cis elements to regulate biotic and abiotic stress responses. here, we report that an erf transcription factor gene (gberf2) was cloned by suppression subtraction hybridization from sea-island cotton after verticillium dahliae attack. the gberf2 cdna has a total length of 1143 bp with an open reading frame of 597 bp. the genomic sequence of gberf2 contains an intron of 515 bp. the gene encodes a predicated polypeptide of 198 amino acids wit ...	2007	17321073
signaling pathways controlling induced resistance to insect herbivores in arabidopsis.	insect attack triggers changes in transcript level in plants that are mediated predominantly by jasmonic acid (ja). the implication of ethylene (et), salicylic acid (sa), and other signals in this response is less understood and was monitored with a microarray containing insect- and defense-regulated genes. arabidopsis thaliana mutants coi1-1, ein2-1, and sid2-1 impaired in ja, et, and sa signaling pathways were challenged with the specialist small cabbage white (pieris rapae) and the generalist ...	2007	17977152
a transgressive segregation factor (rkn2) in gossypium barbadense for nematode resistance clusters with gene rkn1 in g. hirsutum.	host plant resistance is an important strategy for managing root-knot nematode (meloidogyne incognita) in cotton (gossypium l.). here we report evidence for enhanced resistance in interspecific crosses resulting from transgressive segregation of clustered gene loci. recently, a major gene, rkn1, on chromosome 11 for resistance to m. incognita in cv. acala nemx was identified using an intraspecific g. hirsutum cross with susceptible cv. acala sj-2. using interspecific crosses of acala nemxxsuscep ...	2008	17940800
molecular cloning and characterization of the actin-depolymerizing factor gene in gossypium barbadense.	the sea island cotton (gossypium barbadense l.) has been highly valued in verticillium wilt resistance and many fiber qualities including fiber length, strength and fineness. to identify whether it had some special genes in fiber development in comparison with the upland cotton (g. hirsutum l.), an actin-depolymerizing factor (adf) gene was cloned and characterized in this research. a 420 bp open reading frame of the cloned gene, named gbadf1, encoded a protein of 139 amino acids, including 39.5 ...	2008	19168989
starch grains on human teeth reveal early broad crop diet in northern peru.	previous research indicates that the nanchoc valley in northern peru was an important locus of early and middle holocene human settlement, and that between 9200 and 5500 (14)c yr b.p. the valley inhabitants adopted major crop plants such as squash (cucurbita moschata), peanuts (arachis sp.), and cotton (gossypium barbadense). we report here an examination of starch grains preserved in the calculus of human teeth from these sites that provides direct evidence for the early consumption of cultivat ...	2008	19066222
plant crude extracts could be the solution: extracts showing in vivo antitumorigenic activity.	screening active compounds from plants lead to discover new medicinal drugs which have efficient protection and treatment roles against various diseases including cancer. in our study, extracts from different plants represent seeds of: gossypium barbadense, ricinus communis, sesamum indicum, nigella sativa, vinca rosea and melia azedarah; fruits of: xanthium occidental; flowers of: atriplex nummularia; barks of: cinnamomum zeylanicum; latex of: ficus carica and rhizomes of: curcuma longa and zin ...	2008	18390447
global analysis of gene expression in cotton fibers from wild and domesticated gossypium barbadense.	gossypium barbadense is widely cultivated because of its extra-long staple cotton with superior luster, silkiness and high yield. these economically important traits were selected during initial domestication of an agronomically inferior wild ancestor, followed by millennia of human-mediated selection. to reveal the effects of this history on the cotton fiber transcriptome, we conducted comparative expression profiling on mechanically isolated fiber cells at three different stages encompassing e ...	2008	18803775
[cotton fiber quality traits were controlled mainly by maternal plant genotype].	hai1, a gossypium barbadense l. variety with super fiber quality, and ccri36 and zhong221, two upland cotton cultivars (gossypium hirsutum l.), were used as recurrent parents to develop two backcross combinations of ccri36xhai1 and zhong221xhai1. fiber quality of inter-crossing bolls and self-crossing bolls were analyzed from different generations of the two combinations. the results showed that there existed significant difference in the average value, pole difference and cv% of fiber quality t ...	2008	19073557
identification of differentially expressed genes associated with cotton fiber development in a chromosomal substitution line (cs-b22sh).	one of the impediments in the genetic improvement of cotton fiber is the paucity of information about genes associated with fiber development. availability of chromosome arm substitution line cs-b22sh (chromosome 22 short arm substitution from 3-79 (gossypium barbadense) into a tm-1 (gossypium hirsutum) background) provides a novel opportunity to study fiber-associated genes because previous studies revealed this line was associated with some superior fiber quality traits compared to tm-1. we us ...	2008	18043952
hplc preparation of the chiral forms of 6-methoxy-gossypol and 6,6'-dimethoxy-gossypol.	a concentrated mixture of gossypol, 6-methoxy-gossypol, and 6,6'-dimethoxy-gossypol was extracted from the root bark of st. vincent sea island cotton with acetone. this extract was derivatized with r-(-)-2-amino-1-propanol to form diastereomeric gossypol schiff's bases. analytical-scale reverse-phase chromatography of these schiff's bases produced six peaks, indicating separation of the enantiomeric forms of the three gossypol compounds. the elution order of the peaks was found to vary with the ...	2008	18407810
sampling nucleotide diversity in cotton.	cultivated cotton is an annual fiber crop derived mainly from two perennial species, gossypium hirsutum l. or upland cotton, and g. barbadense l., extra long-staple fiber pima or egyptian cotton. these two cultivated species are among five allotetraploid species presumably derived monophyletically between g. arboreum and g. raimondii. genomic-based approaches have been hindered by the limited variation within species. yet, population-based methods are being used for genome-wide introgression of ...	2009	19840401
[analysis on linkage inheritance of sub-red plant mutant from upland cotton].	analysis of linkage inheritance of sub-red plant, a natural mutant from upland cotton, was conducted by two-point test using parents gossypium barbadense hai7124 and g. hirsutum b026 both with petal spots and green leaves, and g. hirsutum e083 with normal white flowers and, sub-red plants. the average percentage of recombination was 1.35% when calculating test-cross combining populations derived from e083/b026//su9701 and b026/e083//su9701. the average percentage of recombination was 2.94% when ...	2009	19689948
genetic changes in plant growth and their associations with chromosomes from gossypium barbadense l. in g. hirsutum l.	cotton (gossypium spp.) plant growth is an important time-specific agronomic character that supports the development of squares, flowers, boll retention, and yield. with the use of a mixed linear model approach, we investigated 14 cotton chromosome substitution (cs-b) lines and their chromosome-specific f(2) hybrids for genetic changes in plant growth that was measured during the primary flowering time under two environments. the changes in additive and dominance variances for plant height and n ...	2009	19153810
total and percent atropisomers of gossypol and gossypol-6-methyl ether in seeds from pima cottons and accessions of gossypium barbadense l.	gossypol occurs naturally in the seed, foliage, and roots of the cotton plant ( gossypium ) as atropisomers due to restricted rotation around the binaphthyl bond. the atropisomers differ in their biological activities. (-)-(r)-gossypol is more toxic and exhibits significantly greater anticancer activity than the (+)-(s)-atropisomer. most commercial upland ( gossypium hirsutum ) cottonseeds have an (r)- to (s)-gossypol ratio of approximately 2:3, but some pima ( gossypium barbadense ) seeds have ...	2009	19113939
peripheral modulation of olfaction by physiological state in the egyptian leaf worm spodoptera littoralis (lepidoptera: noctuidae).	insects show behavioural plasticity based on their physiological state. deprivation from a resource will normally make them more responsive to it or to perform behaviour increasing the probability of encountering such a resource. modulation of the olfactory system has been shown mainly in the central nervous system, but also in the periphery. in this study, antennal sensitivity of females of the egyptian cotton leaf worm spodoptera littoralis to different plant and sex pheromone odours was measu ...	2009	19414013
clock-controlled rhythm of ecdysteroid levels in the haemolymph and testes, and its relation to sperm release in the egyptian cotton leafworm, spodoptera littoralis.	in spodoptera littoralis, testicular sperm release occurs in a daily rhythm, which is controlled by endogenous circadian oscillator located in the male reproductive system. although this rhythm is essential for male fertility, factors that initiate and maintain daily sperm release are not understood. in this study, we investigated a modulatory role for ecdysteroids in the sperm release rhythm and identified the source of ecdysteroids in adult males. we found that the onset of sperm release occur ...	2009	19233333
identification and expression profile of gbagl2, a c-class gene from gossypium barbadense.	an agamous (ag)-like gene, gbagl2, was isolated from gossypium barbadense and characterized. alignment and phylogenetic analysis indicated that gbagl2 shared high homology with ag-subfamily genes and belonged to a c-class gene family. dna gel blot analysis showed that gbagl2 belonged to a low-copy gene family. reverse transcriptase-polymerase chain reaction (rt-pcr) and quantitative real-time pcr (qpcr) revealed that gbagl2 was highly expressed in reproductive tissues including ovules and carpel ...	2009	20093747
the effects of different prey regimes on the proteolytic digestion of nymphs of the spined soldier bug, podisus maculiventris (hemiptera: pentatomidae).	the effects of different prey regimes on the performance and digestive physiology of the spined soldier bug, podisus maculiventris (say) (hemiptera: pentatomidae), were assessed. specifically, p. maculiventris nymphs were fed on colorado potato beetle (cpb), leptinotarsa decemlineata say (coleoptera: chrysomelidae), larvae; egyptian cotton leafworm (ecw); spodoptera littoralis (boisduval) (lepidoptera: noctuidae); larvae; calliphora spp. (cal) (diptera: calliphoridae) pupae or a mixture of the t ...	2009	19203402
molecular cloning, expression profiling and functional analyses of a cdna encoding isopentenyl diphosphate isomerase from gossypium barbadense.	gossypol, a type of plant defence sesquiterpenoid phytoalexin, is synthesized from the mep (2c-methyl-d-erythritol 4-phosphate) and mva (mevalonate) pathway in the isoprenoid biosynthetic system. the key step is the isomerization of ipp (isopentenyl diphosphate) to dmapp (dimethylallyl diphosphate), which is catalysed by ipi (ipp isomerase; ec 5.3.3.2). a full-length cdna encoding ipi (designated gbipi) was cloned from gossypium barbadense by race (rapid amplification of cdna ends). the full-len ...	2009	19055484
potential of "lure and kill" in long-term pest management and eradication of invasive species.	"lure and kill" technology has been used for several decades in pest management and eradication of invasive species. in lure and kill, the insect pest attracted by a semiochemical lure is not "entrapped" at the source of the attractant as in mass trapping, but instead the insect is subjected to a killing agent, which eliminates affected individuals from the population after a short period. in past decades, a growing scientific literature has been published on this concept. this article provides ...	2009	19610395
the phytohormone precursor opda is isomerized in the insect gut by a single, specific glutathione transferase.	oxylipins play important roles in stress signaling in plants. the compound 12-oxophytodienoic acid (cis-opda) is an early biosynthetic precursor of jasmonic acid (ja), the key phytohormone orchestrating the plant anti-herbivore defense. when consumed by feeding lepidopteran larvae, plant-derived cis-opda suffers rapid isomerization to iso-opda in the midgut and is excreted in the frass. unlike opda epimerization (yielding trans-opda), the formation of iso-opda is enzyme-dependent, and is catalyz ...	2009	19805297
a thaumatin-like protein gene involved in cotton fiber secondary cell wall development enhances resistance against verticillium dahliae and other stresses in transgenic tobacco.	for the first time, a sea-island cotton (gossypium barbadense l.) thaumatin-like protein gene (gbtlp1) with a potential role in secondary cell wall development has been overexpressed in tobacco to elucidate its function. the presence of the transgene was verified by southern blotting and higher expression levels of gbtlp1 in transgenic tobacco plants were revealed by reverse-transcription and quantitative real-time polymerase chain reaction analyses. transgenic plants with constitutively higher ...	2010	20097164
preliminary studies for the development of a second generation granulocytapheresis (g-cap) column.	the preliminary studies for developing a second generation granulocytapheresis (g-cap) column were made. in the past, the g-cap column has been used for the treatment of ulcerative colitis and rheumatoid arthritis. however, recent clinical studies have revealed that the therapeutic effects of the g-cap column are not significant compared with those of the sham column. these results were considered to be due to insufficient reduction of granulocytes. thus, development of a better granulocyte remo ...	2010	20364286
molecular cloning and expression analysis of a novel sant/myb gene from gossypium barbadense.	myb family transcription factors are implicated in multiple developmental processes. herein, a new full-length cdna encoding a sant/myb transcription factor (designated as gbrl2) was cloned and characterized from cotton (gossypium barbadense l.) for the first time. the full-length cdna of gbrl2 was 573 bp with a 240 bp open reading frame (orf) encoding a deduced protein of 80 amino acid polypeptide with a calculated molecular mass of 8.96 kda and an isoelectric point of 8.96. sequence alignment ...	2010	21069464
opportunistic nature of the mammalian microsporidia: experimental transmission of trachipleistophora extenrec (fungi: microsporidia) between mammalian and insect hosts.	spores of trachipleistophora extenrec, originally isolated from the muscles of the madagascan insectivore hemicentetes semispinosus and maintained by serial passage in severe combined immunodeficiency (scid) mice, were fed to larvae of the egyptian cotton leafworm spodoptera littoralis. extensive infection of larval tissues ensued and caused larval and pupal mortality. the development of t. extenrec in the insect host, studied both by light and electron microscopy, followed generally the same li ...	2010	21188601
expression of s-adenosyl methionine decarboxylase gene for polyamine accumulation in egyptian cotton giza 88 and giza 90.	developing drought tolerance in egyptian cotton varieties is a strategic goal considering the need to expand cotton cultivated area and water scarcity in the nile valley. in the present study, increasing levels of polyamine accumulation via expressing s-adenosyl methionine decarboxylase (samdc) gene was the main goal. samdc cdna isolated from saccharomyces cerevisiae isolate was isolated and genetically engineered into egyptian cotton varieties giza 88 as an extra long staple and giza 90 as a lo ...	2010	21844681
study of the bacillus thuringiensis vip3aa16 histopathological effects and determination of its putative binding proteins in the midgut of spodoptera littoralis.	the bacterium bacillus thuringiensis produces, at the vegetative stage of its growth, vip3a proteins with activity against a broad spectrum of lepidopteran insects. the egyptian cotton leaf worm (spodoptera littoralis) is an important agricultural pest that is susceptible to the vip3aa16 protein of bacillus thuringiensis kurstaki strain bupm95. the midgut histopathology of vip3aa fed larvae showed vacuolization of the cytoplasm, brush border membrane destruction, vesicle formation in the apical ...	2010	20965198
synthesis of hemigossypol and its derivatives.	hemigossypol (3), a sesquiterpene natural product, was previously isolated from gossypium barbadense and was shown to display improved anti-fungal activity compared to gossypol (1), the disesquiterpene dimer of hemigossypol (3). gossypol exhibits multiple biological activities. in order to study whether hemigossypol and it derivatives retain the various bioactivities of gossypol, we developed a short and convenient synthetic scheme to synthesize hemigossypol. this is the first de novo synthesis ...	2010	22837586
an l1 box binding protein, gbml1, interacts with gbmyb25 to control cotton fibre development.	transcription factors play key roles in plant development through their interaction with cis-elements and/or other transcription factors. a hd-zip iv family transcription factor, gossypium barbadense meristem layer 1 (gbml1) has been identified and characterized here. gbml1 specifically bound to the l1 box and the promoters of gbml1 and gbrdl1. gbml1 physically interacted with a key regulator of cotton fibre development, gbmyb25. truncated and point mutation assays indicated the start-sad domain ...	2010	20667961
chlorpyrifos exposures in egyptian cotton field workers.	neurobehavioral deficits have been reported in egyptian pesticide application teams using organophosphorus (op) pesticides, but whether these effects are related to op pesticide exposures has yet to be established. in preparation for a comprehensive study of the relationship between op pesticide dose and neurobehavioral deficits, we assessed exposure within this population. we conducted occupational surveys and workplace observations, and collected air, dermal patch and biological samples from a ...	2010	20193710
genetical and biological control of cotton ashy stem caused by macrophomina phaseolina in outdoor pot experiment.	two outdoor pot experiments were carried out to evaluate the reaction of 11 commercial egyptian cotton cultivars macrophomina phaseolina, the incitant of ashy stem in cotton and to evaluate the antagonistic ability of 27 isolates of trichoderma sp. against pathogen cotton cultivars giza 85, giza 87, giza 89 and giza 90 were resistant to m. phaseolina because both survival and plant height of these cultivars was not affected when the soil was infested with the pathogen. none of the cultivars were ...	2010	23961071
genetic dissection of chromosome substitution lines of cotton to discover novel gossypium barbadense l. alleles for improvement of agronomic traits.	we recently released a set of 17 chromosome substitution (cs-b) lines (2n = 52) that contain gossypium barbadense l. doubled-haploid line '3-79' germplasm systematically introgressed into the upland inbred 'tm-1' of g. hirsutum (l.). tm-1 yields much more than 3-79, but cotton from the latter has superior fiber properties. to explore the use of these quasi-isogenic lines in studying gene interactions, we created a partial diallel among six cs-b lines and the inbred tm-1, and characterized their ...	2010	20094702
ethnomedicine and ethnobotany of fright, a caribbean culture-bound psychiatric syndrome.	"fright" is an english-speaking caribbean idiom for an illness, or ethnomedical syndrome, of persistent distress. a parallel ethnopsychiatric idiom exists in the french antilles as sésisma. fright is distinct from susto among hispanics, though both develop in the wake of traumatic events. west indian ethnophysiology (ethnoanatomy) theorizes that an overload of stressful emotions (fear, panic, anguish or worry) causes a cold humoral state in which blood coagulates causing prolonged distress and i ...	2010	20163730
functional analysis of gbagl1, a d-lineage gene from cotton (gossypium barbadense).	cotton fibres originate from the outer ovule integument and d-lineage genes are essential for ovule development and their roles can be described by the 'abcde' model of flower development. to investigate the role of d-lineage genes during ovule and fibre development, gbagl1 (genbank accession number: fj198049) was isolated from g. barbadense by using the smart race strategy. sequence and phylogenetic analyses revealed that gbagl1 was a member of the d-lineage gene family. southern blot analysis ...	2010	20054032
among-sampler variation in sweep net samples of adult lygus hesperus (hemiptera: miridae) in cotton.	the sweep net is a standard sampling method for adults of the western tarnished plant bug, lygus hesperus knight (hemiptera: miridae), in cotton (gossypium spp.). however, factors that influence the relationship between true population levels and population estimates obtained using the sweep net are poorly documented. improved understanding of these factors is needed for the development and application of refined treatment thresholds. recent reports of significant among-sampler differences in sw ...	2011	21510222
identification of differentially expressed genes associated with semigamy in pima cotton (gossypium barbadense l.) through comparative microarray analysis.	semigamy in cotton is a type of facultative apomixis controlled by an incompletely dominant autosomal gene (se). during semigamy, the sperm and egg cells undergo cellular fusion, but the sperm and egg nucleus fail to fuse in the embryo sac, giving rise to diploid, haploid, or chimeric embryos composed of sectors of paternal and maternal origin. in this study we sought to identify differentially expressed genes related to the semigamy genotype by implementing a comparative microarray analysis of ...	2011	21410961
structure, expression differentiation and evolution of duplicated fiber developmental genes in gossypium barbadense and g. hirsutum.	both gossypium hirsutum and g. barbadense probably originated from a common ancestor, but they have very different agronomic and fiber quality characters. here we selected 17 fiber development-related genes to study their structures, tree topologies, chromosomal location and expression patterns to better understand the interspecific divergence of fiber development genes in the two cultivated tetraploid species.	2011	21349199
biomarkers of chlorpyrifos exposure and effect in egyptian cotton field workers.	chlorpyrifos (cpf), a widely used organophosphorus pesticide (op), is metabolized to cpf-oxon, a potent cholinesterase (che) inhibitor, and trichloro-2-pyridinol (tcpy). urinary tcpy is often used as a biomarker for cpf exposure, whereas blood che activity is considered an indicator of cpf toxicity. however, whether these biomarkers are dose related has not been studied extensively in populations with repeated daily op exposures.	2011	21224175
metabolite and mineral analyses of cotton near-isogenic lines introgressed with qtls for productivity and drought-related traits.	quantitative trait loci (qtls) for yield and drought-related traits were exchanged via marker-assisted selection between elite cultivars of two cotton species, gossypium barbadense (gb) cv. f-177 and gossypium hirsutum (gh) cv. siv'on. three of the resultant near-isogenic lines (nils), each introgressed with a different qtl region, expressed an advantage in osmotic adjustment (oa) and other drought-related traits relative to their recipient parents. these nils and the parental genotypes were fie ...	2011	21143238
comparative expression of mirna genes and mirna-based aflp marker analysis in cultivated tetraploid cottons.	micrornas (mirnas) are a class of small non-coding rnas that down-regulate gene expression in a sequence specific manner to control plant growth and development. the identification and characterization of mirnas are critical steps in finding their target genes and elucidating their functions. the objective of the present study was to assess the genetic variation of mirna genes through expression comparisons and mirna-based aflp marker analysis. seven mirnas were first selected for rt-pcr and fou ...	2011	21134704
genetic diversity of sea-island cotton (gossypium barbadense) revealed by mapped ssrs.	in order to evaluate the genetic diversity of sea-island cotton (gossypium barbadense), 237 commonly mapped ssr markers covering the cotton genome were used to genotype 56 sea-island cotton accessions. a total of 218 polymorphic primer pairs (91.98%) amplified 361 loci, with a mean of 1.66 loci. polymorphism information content values of the ssr primers ranged from 0.035 to 0.862, with a mean of 0.320. the highest mean polymorphism information content value for the ssr motifs was from a compound ...	2011	22183945
individual chromosome assignment and chromosomal collinearity in gossypium thurberi, g. trilobum and d subgenome of g. barbadense revealed by bac-fish.	the experiment on individual chromosome assignments and chromosomal diversity was conducted using a multi-probe fluorescence in situ hybridization (fish) system in d subgenome of tetraploid gossypium barbadense (d(b)), g. thurberi (d(1)) and g. trilobum (d(8)), which the later two were the possible subgenome donors of tetraploid cottons. the fish probes contained a set of bacterial artificial chromosome (bac) clones specific to 13 individual chromosomes from d subgenome of g. hirsutum (d(h)), a ...	2011	21952206
generation, annotation and analysis of first large-scale expressed sequence tags from developing fiber of gossypium barbadense l.	cotton fiber is the world's leading natural fiber used in the manufacture of textiles. gossypium is also the model plant in the study of polyploidization, evolution, cell elongation, cell wall development, and cellulose biosynthesis. g. barbadense l. is an ideal candidate for providing new genetic variations useful to improve fiber quality for its superior properties. however, little is known about fiber development mechanisms of g. barbadense and only a few molecular resources are available in ...	2011	21829504
The impact of the Bacillus subtilis SPB1 biosurfactant on the midgut histology of Spodoptera littoralis (Lepidoptera: Noctuidae) and determination of its putative receptor.	SPB1 is a Bacillus subtilis strain producing a lipopeptide biosurfactant. The insecticidal activity of this biosurfactant was evaluated against the Egyptian cotton leaf worm (Spodoptera littoralis). It displayed toxicity with an LC(50) of 251ng/cm(2). The histopathological changes occurred in the larval midgut of S. littoralis treated with B. subtilis SPB1 biosurfactant were vesicle formation in the apical region, cellular vacuolization and destruction of epithelial cells and their boundaries. L ...	2011	22079884
preliminary studies for the development of a second generation granulocytapheresis (g-cap) column. part ii: in vitro and ex vivo studies.	our previous studies concluded egyptian cotton was the most appropriate material for making a second generation granulocytapheresis (g-cap) column as structural dimensions of the cotton fibers are able to attract granulocytes. unfortunately, it is considered to be blood incompatible as its fibers are of non-synthetic origins. in this study we examined the alteration of the removal rates of blood cells with different surface modifications of egyptian cotton to enhance its blood compatibility. the ...	2011	21713519
investigation of the steps involved in the difference of susceptibility of ephestia kuehniella and spodoptera littoralis to the bacillus thuringiensis vip3aa16 toxin.	bupm95 is a bacillusthuringiensis subsp. kurstaki strain producing the vip3aa16 toxin with an interesting insecticidal activity against the lepidopteran larvae ephestia kuehniella. study of different steps in the mode of action of this vegetative insecticidal protein on the mediterranean flour moth (e. kuehniella) was carried out in the aim to investigate the origin of the higher susceptibility of this insect to vip3aa16 toxin compared to that of the egyptian cotton leaf worm spodoptera littoral ...	2011	21600212
identification of qtl regions and ssr markers associated with resistance to reniform nematode in gossypium barbadense l. accession gb713.	the identification of molecular markers that are closely linked to gene(s) in gossypium barbadense l. accession gb713 that confer a high level of resistance to reniform nematode (rn), rotylenchulus reniformis linford & oliveira, would be very useful in cotton breeding programs. our objectives were to determine the inheritance of rn resistance in the accession gb713, to identify ssr markers linked with rn resistance qtls, and to map these linked markers to specific chromosomes. we grew and scored ...	2011	20845024
identification, phylogenetic analysis and expression profile of an anionic insect defensin gene, with antibacterial activity, from bacterial-challenged cotton leafworm, spodoptera littoralis.	defensins are a well known family of cationic antibacterial peptides (amps) isolated from fungi, plants, insects, mussels, birds, and various mammals. they are predominantly active against gram (+) bacteria, and a few of them are also active against gram (-) bacteria and fungi. all insect defensins belonging to the invertebrate class have a consensus motif, c-x₅₋₁₆-c-x₃-c-x₉₋₁₀-c-x₄₋₇-cx₁-c. only seven amps have already been found in different lepidopteran species. no report was published on the ...	2011	22067477
mosquito feeding affects larval behaviour and development in a moth.	organisms are attacked by different natural enemies present in their habitat. while enemies such as parasitoids and predators will kill their hosts/preys when they successfully attack them, enemies such as micropredators will not entirely consume their prey. however, they can still have important consequences on the performance and ecology of the prey, such as reduced growth, increased emigration, disease transmission. in this paper, we investigated the impact of a terrestrial micropredator, the ...	2011	21991329
cloning and characterization of a verticillium wilt resistance gene from gossypium barbadense and functional analysis in arabidopsis thaliana.	verticillium wilt causes enormous loss to yield or quality in many crops. in an effort to help controlling this disease through genetic engineering, we first cloned and characterized a verticillium wilt resistance gene (gbve) from cotton (gossypium barbadense) and analyzed its function in arabidopsis thaliana. its nucleotide sequence is 3,819-ábp long, with an open reading frame of 3,387-ábp, and encoding an 1,128-aa protein precursor. sequence analysis shows that gbve produces a leucine-rich re ...	2011	21739145
Proteomic analysis of the sea-island cotton roots infected by wilt pathogen Verticillium dahliae.	Verticillium wilt of cotton is a vascular disease mainly caused by the soil-born filamentous fungus Verticillium dahliae. To study the mechanisms associated with defense responses in wilt-resistant sea-island cotton (Gossypium barbadense) upon V. dahliae infection, a comparative proteomic analysis between infected and mock-inoculated roots of G. barbadense var. Hai 7124 (a cultivar showing resistance against V. dahliae) was performed by 2-DE combined with local EST database-assisted PMF and MS/M ...	2011	21928292
lignin metabolism has a central role in the resistance of cotton to the wilt fungus verticillium dahliae as revealed by rna-seq-dependent transcriptional analysis and histochemistry.	the incompatible pathosystem between resistant cotton (gossypium barbadense cv. 7124) and verticillium dahliae strain v991 was used to study the cotton transcriptome changes after pathogen inoculation by rna-seq. of 32 774 genes detected by mapping the tags to assembly cotton contigs, 3442 defence-responsive genes were identified. gene cluster analyses and functional assignments of differentially expressed genes indicated a significant transcriptional complexity. quantitative real-time pcr (qpcr ...	2011	21862479
overexpression of gbwrky1 positively regulates the pi starvation response by alteration of auxin sensitivity in arabidopsis.	overexpression of a cotton defense-related gene gbwrky1 in arabidopsis resulted in modification of the root system by enhanced auxin sensitivity to positively regulate the pi starvation response. gbwrky1 was a cloned wrky transcription factor from gossypium barbadense, which was firstly identified as a defense-related gene and showed moderate similarity with atwrky75 from arabidopsis thaliana. overexpression of gbwrky1 in arabidopsis resulted in attenuated pi starvation stress symptoms, includin ...	2012	22890372
cloning of a phosphatidylinositol 4-kinase gene based on fiber strength transcriptome qtl mapping in the cotton species gossypium barbadense.	sea island cotton (gossypium barbadense) is highly valued for its superior fiber qualities, especially fiber strength. based on a transcript-derived fragment originated from transcriptome qtl mapping, a fiber strength related candidate gene of phosphatidylinositol 4-kinase cdna, designated as gbpi4k, was first cloned, and its expression was characterized in the secondary cell wall thickening stage of g. barbadense fibers. the orf of gbpi4k was found to be 1926 bp in length and encoded a predicte ...	2012	22869083
laboratory evaluation of isaria fumosorosea against spodoptera littoralis.	isaria fumosorosea (syn. paecilomyces fumosoroseus) is potentially useful for the biological control of economically important agricultural and forest insect pests. we evaluated efficacy of two strains of this entomopathogenic fungus against last instar larvae and pupae of egyptian cotton leafworm, spodoptera littoralis. the first strain was apopka 97 which is an active ingredient of commercial biopesticide preferal wg (biobest, belgium). the second strain was isolated from cameraria ohridella a ...	2012	23885438
repeated polyploidization of gossypium genomes and the evolution of spinnable cotton fibres.	polyploidy often confers emergent properties, such as the higher fibre productivity and quality of tetraploid cottons than diploid cottons bred for the same environments. here we show that an abrupt five- to sixfold ploidy increase approximately 60 million years (myr) ago, and allopolyploidy reuniting divergent gossypium genomes approximately 1-2 myr ago, conferred about 30-36-fold duplication of ancestral angiosperm (flowering plant) genes in elite cottons (gossypium hirsutum and gossypium barb ...	2012	23257886
the draft genome of a diploid cotton gossypium raimondii.	we have sequenced and assembled a draft genome of g. raimondii, whose progenitor is the putative contributor of the d subgenome to the economically important fiber-producing cotton species gossypium hirsutum and gossypium barbadense. over 73% of the assembled sequences were anchored on 13 g. raimondii chromosomes. the genome contains 40,976 protein-coding genes, with 92.2% of these further confirmed by transcriptome data. evidence of the hexaploidization event shared by the eudicots as well as o ...	2012	22922876
cotton leaf curl multan betasatellite strains cloned from gossypium barbadense further supports selection due to host resistance.	the cotton leaf curl multan betasatellite encodes an essential pathogenicity determinant involved in induction of disease symptoms. we have shown recently that a recombinant betasatellite with a satellite conserved region derived from the tomato leaf curl betasatellite, is prevalent in the punjab province and is associated with the breakdown of resistance in cotton to cotton leaf curl disease. we intended to see if the betasatellite that was associated with the first epidemic is still being main ...	2012	22644763
[cloning and functional analysis of chitinase gene gbchi from sea-island cotton (gossypium barbadense)].	chitinase is one of the important pathogenesis-related (pr) proteins in plants. by comparative proteomics study, a novel pathogen-responsive chitinase (known as gbchi) has been identified from sea-island cotton (gossypium barbadense). the gbchi cdna was cloned from wilt-resistant sea-island cotton and the anti-fungal activity of the gene product was investigated. qrt-pcr analysis indicated that gbchi was expressed constitutively in root, stem, leaf, flower, and ovule of cotton plant, and the exp ...	2012	22382066
molecular evolution and phylogenetic analysis of genes related to cotton fibers development from wild and domesticated cotton species in gossypium.	the domestication of both diploid and tetraploid cotton species was carried out for fiber utilization. to understand the origin and domestication of fibers, 18 genes related to fiber development were individually cloned and sequenced from 22 different cotton species. their structures, phylogenetic relationship and molecular evolution were further studied. in the orthologous and homeologous loci of the 18 genes, the sequence and structure of 72.22% were conserved and 27.78% were diverse. tree top ...	2012	22381639
archaeogenomic evidence of punctuated genome evolution in gossypium.	transposable elements (tes) are drivers of evolution resulting in episodic surges of genetic innovation and genomic reorganization (oliver kr, greene wk. 2009. tes: powerful facilitators of evolution. bioessays 31:703-714.), but there is little evidence of the timescale in which this process has occurred (gingerich pd. 2009. rates of evolution. ann rev ecol evol syst. 40:657-675.). the paleontological and archaeological records provide direct evidence for how evolution has proceeded in the past, ...	2012	22334578
inheritance of long staple fiber quality traits of gossypium barbadense in g. hirsutum background using csils.	gossypium hirsutum is a high yield cotton species that exhibits only moderate performance in fiber qualities. a promising but challenging approach to improving its phenotypes is interspecific introgression, the transfer of valuable traits or genes from the germplasm of another species such as g. barbadense, an important cultivated extra long staple cotton species. one set of chromosome segment introgression lines (csils) was developed, where tm-1, the genetic standard in g. hirsutum, was used as ...	2012	22297564
genome-wide profiling of mirnas and other small non-coding rnas in the verticillium dahliae-inoculated cotton roots.	micrornas (mirnas) and small interfering rnas (sirnas) are short (19-25 nucleotides) non-coding rna molecules that have large-scale regulatory effects on development and stress responses in plants. verticillium wilt is a vascular disease in plants caused by the fungal pathogen verticillium dahliae. the objective of this study is to investigate the transcriptional profile of mirnas and other small non-coding rnas in verticillium-inoculated cotton roots. four small rna libraries were constructed f ...	2012	22558219
gbwrky1, a novel cotton (gossypium barbadense) wrky gene isolated from a bacteriophage full-length cdna library, is induced by infection with verticillium dahliae.	wrky transcription factor proteins play important roles in diverse stress responses. in this study, we first cloned a novel wrky from our constructed bacteriophage full-length cdna library for cotton (gossypium barbadense). the plants were stressed by exposure to a defoliating strain of verticillium dahliae. the capacity of primary cdna library was 1.28 x 10(6) pfu and the titer of the amplified cdna library was > 10(10) pfu ml(-1). the recombination rate of the library was 94% and average inser ...	2012	23350274
overexpression of ghsusa1 increases plant biomass and improves cotton fiber yield and quality.	cotton (gossypium spp.) is an important economic crop and the largest source of textile fiber in the world. however, to date, only a few genes have been identified that exhibit critical roles in fiber development, and few has shown positive effects on fiber yield and quality in transgenic cotton. here, we report the characterization of a novel sucrose synthase (susa1) gene from a superior quality fiber germplasm line 7235 in gossypium hirsutum. by association analysis, ghsusa1 was highly correla ...	2012	22044435
modulation of the temporal pattern of calling behavior of female spodoptera littoralis by exposure to sex pheromone.	we have examined the timing of calling behavior in the female egyptian cotton leafworm, spodoptera littoralis and its modification by exposure to sex pheromone. the calling rhythm of the female moth was found to be circadian, persistent for at least 4 days once it has been entrained, and could be phase shifted by altering the light:dark regime. we also found that female exposure to pheromone affected the rate and duration of calling. a brief exposure to pheromone gland extract increased the prop ...	2012	22001286
isolation of (-)-gossypol from natural plant.	(-)-gossypol has been isolated from cottonseed of g. barbadense l. three gossypium species were studied for the isolation. the study has also revealed a correlation between the optical activity of gossypol isolated and the species of cottonseed used. gossypol isolated from the species g. barbadense is always found to be levorotatory caused by an excess of (-)-gossypol, whereas that from the species g. hirsutum is always dextrorotatory caused by an excess of (+)-gossypol. results of most samples ...	2012	3370996
peripheral regulation by ecdysteroids of olfactory responsiveness in male egyptian cotton leaf worms, spodoptera littoralis.	physiological and behavioral plasticity allows animals to adapt to changes in external (environmental) and internal (physiological) factors. in insects, the physiological state modulates adult behavior in response to different odorant stimuli. hormones have the potential to play a major role in the plasticity of the olfactory responses. to explore if peripheral olfactory processing could be regulated by steroid hormones, we characterized the molecular, electrophysiological, and behavioral respon ...	2012	22044719
genistein effects on haematoimmune cells in a newly developed alternative toxicological model.	unexpected, sometimes opposite effects of dietary isoflavonic phytoestrogens on immunity may suggest that classical mammalian toxicological assays are not entirely suitable for preclinical safety tests of these compounds. we evaluated a new alternative model of haemocytes of egyptian cotton worm in vivo following genistein administration. genistein induced significant changes in nucleolar morphology of haemocytes but did not influence their counts and nucleolar indices. the results indicate that ...	2012	21095109
gbtcp, a cotton tcp transcription factor, confers fibre elongation and root hair development by a complex regulating system.	as the most important natural raw material for textile industry, cotton fibres are an excellent model for studying single-cell development. although expression profiling and functional genomics have provided some data, the mechanism of fibre development is still not well known. a class i tcp transcription factor (designated gbtcp), encoding 344 amino acids, was isolated from the normalized cdna library of sea-island cotton fibre (from -2 to 25 days post anthesis). gbtcp was preferentially expres ...	2012	23105133
analysis of sea-island cotton and upland cotton in response to verticillium dahliae infection by rna sequencing.	cotton verticillium wilt is a serious soil-borne vascular disease that causes great economic loss each year. however, due to the lack of resistant varieties of upland cotton, the molecular mechanisms of resistance to this disease, especially to the pathogen verticillium dahliae, remain unclear.	2013	24314117
characterization and expression analysis of a fiber differentially expressed fasciclin-like arabinogalactan protein gene in sea island cotton fibers.	fasciclin-like arabinogalactan (fla) protein is a cell-wall-associated protein playing crucial roles in regulating plant growth and development, and it was characterized in different plants including upland cotton (gossypium hirsutum l.). in cdna-aflp analysis of 25 dpa (days post anthesis) fiber mrna, two fla gene-related transcripts exhibit differential expression between sea island cotton (g. barbadense l.) and upland cotton. based on the transcript-derived fragment, race-pcr and realtime pcr ...	2013	23875019
truncated cotton subtilase promoter directs guard cell-specific expression of foreign genes in tobacco and arabidopsis.	a 993-bp regulatory region upstream of the translation start codon of subtilisin-like serine protease gene was isolated from gossypium barbadense. this (t/a)aaag-rich region, gbslsp, and its 5'- and 3'-truncated versions were transferred into tobacco and arabidopsis after fusing with gus or gfp. histochemical and quantitative gus analysis and confocal gfp fluorescence scanning in the transgenic plants showed that the gbslsp-driven gus and gfp expressed preferentially in guard cells, whereas driv ...	2013	23555786
why urban citizens in developing countries use traditional medicines: the case of suriname.	the use of traditional medicines (tms) among urban populations in developing countries and factors underlying people's decision to use tms are poorly documented. we interviewed 270 adults in paramaribo, suriname, using a stratified random household sample, semistructured questionnaires, and multivariate analysis. respondents mentioned 144 medicinal plant species, most frequently gossypium barbadense, phyllanthus amarus, and quassia amara. 66% had used tms in the previous year, especially people ...	2013	23653663
demonstration of a diel trend in sensitivity of gossypium to ozone: a step toward relating o₃ injury to exposure or flux.	plant injury by ozone (o3) occurs in three stages, o3 entrance through stomata, overcoming defences, and attack on bioreceptors. concentration, deposition, and uptake of o3 are accessible by observation and modelling, while injury can be assessed visually or through remote sensing. however, the relationship between o3 metrics and injury is confounded by variation in sensitivity to o3. sensitivity weighting parameters have previously been assigned to different plant functional types and growth st ...	2013	23404900
development of agrobacterium-mediated virus-induced gene silencing and performance evaluation of four marker genes in gossypium barbadense.	gossypiumbarbadense is a cultivated cotton species and possesses many desirable traits, including high fiber quality and resistance to pathogens, especially verticilliumdahliae (a devastating pathogen of gossypium hirsutum, the main cultivated species). these elite traits are difficult to be introduced into g. hirsutum through classical breeding methods. in addition, genetic transformation of g. barbadense has not been successfully performed. it is therefore important to develop methods for eval ...	2013	24023833
a receptor-like kinase gene (gbrlk) from gossypium barbadense enhances salinity and drought-stress tolerance in arabidopsis.	cotton (gossypium spp.) is widely cultivated due to the important economic value of its fiber. however, extreme environmental degradation impedes cotton growth and production. receptor-like kinase (rlk) proteins play important roles in signal transduction and participate in a diverse range of processes in response to plant hormones and environmental cues. here, we introduced an rlk gene (gbrlk) from cotton into arabidopsis and investigated its role in imparting abiotic stress tolerance.	2013	23915077
determination and analysis of the genome sequence of spodoptera littoralis multiple nucleopolyhedrovirus.	the spodoptera littoralis multiple nucleopolyhedrovirus (splimnpv), a pathogen of the egyptian cotton leaf worm s. littoralis, was subjected to sequencing of its entire dna genome and bioassay analysis comparing its virulence to that of other baculoviruses. the annotated splimnpv genome of 137,998 bp was found to harbor 132 open reading frames and 15 homologous repeat regions. four unique genes not present in spltmnpv were identified, as were 14 genes that were absent or translocated by comparis ...	2013	23219924