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ultrastructure of mycoplasmatales virus laidlawii i. 19724116131
glutamate dehydrogenase from mycoplasma laidlawii.mycoplasma laidlawii possesses a single glutamate dehydrogenase (gdh) with dual coenzyme specificity [specificity for nicotinamide adenine dinucleotide (h) and nicotinamide adenine dinucleotide phosphate (h)]. a purification procedure is reported which results in an enzyme preparation with a specific activity of 79.5 units/mg and which displays only one significant protein band after gel electrophoresis. this one band was determined, by activity staining, to have all of the gdh nucleotide specif ...19724112954
electron microscope observations on acholeplasma laidlawii viruses. 19724114278
silicotungstate negative staining of acholeplasma laidlawii membranes and lipids. 19724118305
lack of interaction between sendai virus and bacterial cells. 19724338794
characterization of the myoplasma membrane proteins. 3. gel filtration and immunological characterization of acholeplasma laidlawii membrane proteins. 19724339322
[immunodepressive effect of mycoplasma laidlawii on the formation of humoral antibodies in mice]. 19724344075
plasticity of biological membranes. 19724346307
protein-lipid interactions. 19724592575
detection of cholesterol in cell membranes by use of bacterial toxins.a method is described for the detection of cholesterol in membranes from erythrocytes, mycoplasmas, and bacterial cells by a ferritin-labeling technique. membranes treated with cereolysin, a bacterial hemolysin which specifically binds to cholesterol, and then treated with ferritin-antitetanolysin, were specifically ferritin-labeled for cholesterol. a similar antigen-antibody system, streptolysin o-ferritin-antistreptolysin, was also used successfully with erythrocyte membranes. there was an une ...19724623312
[active glucose transport in mycoplasma laidlawii]. 19724636164
the growth and morphology of acholeplasma (mycoplasma) laidlawii a in different media. 19724505769
photodynamic killing of acholeplasma laidlawii. 19724643094
elimination of mycoplasma contaminants from virus stocks by treatment with nonionic detergents.five nonionic detergents (tweens 20, 40, 60, and 80, and triton wr-1339) were tested for their ability to inactivate four mycoplasma species which are common contaminants of animal cell cultures. tween 20 was found to be the most effective, in that a concentration of 2.5 mg/ml completely inactivated cultures of m. hominis, m. hyorhinis, and acholeplasma laidlawii within 1 hr and a culture of m. orale within 3 hr. the other detergents exhibited various degree of activity against the different myc ...19724341521
the structure of a glycerylphosphoryldiglucosyl diglyceride from the lipids of acholeplasma laidlawii strain b.1. the phosphatidylglucose structure proposed previously (smith & henrikson, 1965) for the glucose-containing phospholipid from acholeplasma laidlawii is incorrect. 2. the structure now proposed is 3-(sn-glycerol-3-phosphoryl-6'-[o-alpha-d-glucopyranosyl-(1-->2)-o-alpha-d-glucopyranosyl])- sn-1,2-diglyceride, a new type of bacterial lipid. 3. deacylation of the lipid gave a single water-soluble phosphate ester which could be distinguished on chromatography from synthetic samples of glucosylphosp ...19724345854
the effect of aeration on the growth of acholeplasma (mycoplasma) laidlawii a. 19724553303
[comparative characteristics of methods of culturing mycoplasma and l-forms of bacteria and serological reactions used for their identification]. 19724555023
[mycoplasma infection of cattle]. 19724562052
studies on the biological properties of polyene antibiotics: comparison of other polyenes with filipin in their ability to interact specifically with sterol. 19724565643
eagle's basal medium as a defined medium for mycoplasma studies. 19724571002
[mycoplasma (acholeplasma laidlawii) from cold blood cell cultures]. 19724145171
[influence of thallium acetate on the growth of acholeplasmataceae, mycoplasmataceae, and some bacteria species]. 19724145172
[morphological differentiation of some strains of mycoplasma gallisepticum against different mycoplasma species on solid medium]. 19724145213
the temperature requirements of growth of some mycoplasmas. 19724146501
studies on the biological properties of polyene antibiotics. evidence for the direct interaction of filipin with cholesterol. 19725012767
polyacrylamide-gel electrophoresis of cell proteins of mycoplasma isolated from cattle and horses. 19725017869
lipid mobility in acholeplasma membranes using deuteron magnetic resonance. 19725041152
altered incorporation of nucleic acid precursors by mycoplasma-infected mammalian cells in culture. 19725058910
dark and light repair in ultraviolet-irradiated acholeplasma laidlawii. 19725060072
improved activity sedimentation method. 19725062881
mycoplasma isolations from clinically normal cats. 19725063126
[study of mycoplasma isolated from cases of swine pneumonia]. 19725081406
selective solubilization with tween 20 of membrane proteins from acholeplasma laidlawaii. 19725082994
phase transitions of phospholipid bilayers and membranes of acholeplasma laidlawii b visualized by freeze fracturing electron microscopy. 19725082995
thiopeptin, a new feed-additive antibiotic: biological studies and field trials.thiopeptin is a new antibiotic, produced by streptomyces tateyamensis and developed solely for animal use as a feed additive. the antibiotic content in animal tissue and feed was assayed in terms of the antimicrobial activity against mycoplasma laidlawii a. this antibiotic was found to be relatively nontoxic in rats and mice. in chickens, this antibiotic is excreted into feces within 48 hr of administration and is not absorbed in tissue. it is well tolerated in both broilers and swine and is hig ...19724680812
fungistatic activity of lucerne saponins and digitonin as related to sterols. 19724676387
mycoplasma: growth precipitation as a serodiagnostic method.a serological test is described which is designated the growth precipitation test and is based on the release of diffusible compounds from mycoplasma strains. during growth of mycoplasmas on solid substrates, precipitation lines are formed with antibodies from homologous antisera independent of heat-labile serum factors. the application of the test as a serodiagnostic tool was investigated by performing a complete serological cross-test with 10 mycoplasma strains. the test was compared with the ...19724623280
hydrolysis of alanine oligopeptides by an enzyme located in the membrane of mycoplasma laidlawii. 19724640765
survival of acholeplasma laidlawii a and changes in its dna after irradiation with x- and gamma rays. 19724644259
antibiotics from mycoplasma. i. acholeplasma laidlawii b. 19724645949
transfection mediated by mycoplasmatales viral dna.dna isolated from mycoplasmatales viruses mvl51 and mvgs51 was infectious when mixed with acholeplasma laidlawii bn1-na1(r) cells. infectivity was destroyed by deoxyribonuclease but not by ribonuclease, pronase, or specific antiserum to the virus. host mycoplasma cells were only competent for transfection during late-log growth phase. the rates of the establishment of dnase insensitivity of viral dna transfectants were similar to those of bacteriophage systems. the dose-response curve for transf ...19724508332
[sensitivity of mycoplasmas to spiramycin]. 19724669219
[stimulating effect of langat virus on reproduction of mycoplasma laidlawii in combined infection of a primary culture of chick embryo cells]. 19724672317
a phosphatidyl diglucosyl diglyceride from acholeplasma laidlawii b. 19724643340
relations between lipid composition and permeability of membranes. 19724680245
[iron oxidation by mycoplasma laidlawii]. 19724643941
[size distribution of colony-forming units in a population of mycoplasma laidlawii]. 19724657956
osmolar concentration and fixation of mycoplasmas.broth cultures of acholeplasma laidlawii were fixed with various concentrations of cacodylate-buffered glutaraldehyde. the shape and ultrastructure of the organisms varied with the osmolar concentration of the fixative. when the fixation mixture was hypertonic to the culture medium, ultrathin sections suggested that the cells had shrunk. phosphate buffer, sodium chloride, or sucrose at comparable osmolaities had the same effect as sodium cacodylate. glutaraldehyde itself also contributed to the ...19724555408
permeability and topography of membranes. 19724557530
[studies of the biosynthesis of transfer ribonucleic acids]. 19724560651
recent studies using nuclear magnetic resonance spectroscopy of lipids and biological membranes. 19724580642
thermal-turbidimetric studies of membranes from acholeplasma laidlawii. 19725070088
[morphological properties of mycoplasma laidlawii]. 19725042100
13 c nmr studies of lipids in bilayers and membranes. 19725041946
distribution of mycoplasmas in the non-pneumonic bovine respiratory tract. 19725018036
proton magnetic resonance studies of whole human erythrocyte membranes. 19724622201
characterization of the plasma membrane of mycoplasma laidlawii. ix. isolation and characterization of the membrane polyhexosamine. 19725049002
characterization of the plasma membrane of mycoplasma laidlawii. 8. effect of temperature shift and antimetabolites on k + transport. 19725044055
are mycoplasma membrane proteins affected by variations in membrane fatty acid composition? 19725044067
some studies on the fatty acid composition of total lipids and phosphatidylglycerol from acholeplasma laidlawii b and their relation to the premeability of intact cells of this organism. 19725070656
the effect of cholesterol and epicholesterol incorporation on the permeability and on the phase transition of intact acholeplasma laidlawii cell membranes and derived liposomes. 19725011001
the molecular structure of the membrane of acholeplasma laidlawii. 19725041943
spiroplasmas and acholeplasmas: multiplication in insects.the helical wall-free microorganism, spiroplasma citri, which is associated with citrus stubborn, a disease with no known vector, multiplied in the leafhopper vector of corn stunt but multiplied to higher titer in the vector of aster yellows and decreased the longevity of that insect. acholeplasma laidlawii and a. granularum also multiplied in both leafhoppers.19734796196
acyl carrier protein in mycoplasmas.acyl carrier protein (acp) activity was determined by the malonyl-coenzyme a-co(2) exchange reaction. it was highest in acholeplasma laidlawii, lower in a. granularum, and lowest in a. axanthum. the sterol-requiring mycoplasma species examined showed little or negligible acp activity. a. laidlawii was capable of utilizing pantetheine or coenzyme a but not beta-alanine as precursor for acp synthesis. its acp could thus be labeled by growing the organisms with radioactive coenzyme a. the acp of a. ...19734570599
the effect of the polar headgroup on the lipid-cholesterol interaction: a monolayer and differential scanning calorimetry study. 19734711186
nmr studies of sodium and potassium in various biological tissues. 19734513156
fusion of phospholipid vesicles with viable acholeplasma laidlawii.when vesicles of dipalmitoyl phosphatidylcholine produced by sonication are mixed with acholeplasma laidlawii in neutral buffer, a phenomenon occurs that is most readily explained in terms of fusion of lipid vesicle membrane with the mycoplasma cytoplasmic membrane. the mycoplasma can readily accumulate large quantities of the foreign lipid without loss of viability. the added lipids do not remain in patches but diffuse laterally throughout the mycoplasma membrane.19734515622
polyacrylamide gel electrophoresis of mycoplasma proteins in sodium dodecyl sulphate. 19734723070
freeze-fractured acholeplasma laidlawii membranes: nature of particles observed.freeze-fracture of acholeplasma laidlawii membranes from cells incubated in the presence of puromycin or omission of amino acids reveals a decrease in the number of particles between 50 and 100 angstroms in the hydrophobic fracture plane, which strongly suggests that these particles are protein. additional evidence indicates that they may be involved in substrate transport.19734682132
[antigenic relationship of saprophytic and plant mycoplasmas]. 19734683168
weak association of glucosamine-containing polymer with the acholeplasma laidlawii membrane. 19734684605
chemical composition and serology of mycoplasma pneumoniae lipids. 19734689627
[carbohydrate transport in mycoplasma laidlawii cells]. 19734696486
molecular model for phase transition in biological membranes. 19734705755
circular dichroism analysis of native and reaggregated mycoplasma membranes. 19734706839
studies of sugar transport in acholeplasma laidlawii. 19734772677
divalent cations in native and reaggregated mycoplasma membranes.the mg(2+) content of membranes of several mycoplasma and acholeplasma species varied between 0.88 and 1.98 mug of mg(2+) per mg of protein, depending on the species and on growth conditions. ca(2+) could be detected only when it was added to the growth medium. the mg(2+) content of isolated a. laidlawii membranes could be increased almost threefold by dialysis against 20 mm mg(2+), whereas aggregated a. laidlawii membranes contained about six to eight times more mg(2+) per mg of protein than th ...19734632319
freeze-etch electron microscopy of erythrocytes, acholeplasma laidlawii cells and liposomal membranes after the action of filipin and amphotericin b. 19734690870
on the phosphorylation of sugars in acholeplasma laidlawii. 19734709990
the effect of alterations in fatty acid composition and cholesterol content on the nonelectrolyte permeability of acholeplasma laidlawii b cells and derived liposomes. 19734719142
further studies on the morphology and composition of mycoplasmatales virus-laidlawii 2. 19734735309
isolation of mycoplasmatales virus-laidlawii 3, a new virus infecting acholeplasma laidlawii. 19734736684
selective simultaneous proton magnetic resonance labelling of hydrophilic and hydrophobic regions of phospholipid vesicles. effect of polar and apolar reagents. 19734795847
properties of a fructose-1,6-diphosphate-activated lactate dehydrogenase from acholeplasma laidlawii type a.acholeplasma laidlawii a possesses a nicotinamide adenine dinucleotide (nad)-dependent l(+)-lactate dehydrogenase (ldh) which is activated specifically by low concentrations of fructose-1, 6-diphosphate (fdp). studies with partially purified enzyme show that the kinetic response to fdp is hyperbolic. the enzyme is inhibited by inorganic phosphate, adenosine triphosphate, and high concentrations of reduced nad (nadh). low activity is demonstrable in the absence of fdp at ph 6.0 to 7.2, but fdp is ...19734712565
isolation and purification of tdna from mycoplasma. 19734803254
influence of fatty acid and sterol composition on the lipid phase transition and activity of membrane-bound enzymes in acholeplasma laidlawii. 19734149248
some differences between two viruses infecting acholeplasma laidlawii. 19734120326
infection of acholeplasma laidlawii by mvl51 virus. 19734125688
characterization of mycoplasmatales virus dna. 19734573075
are cell membranes fluid? 19734574849
[a comparative analysis of the behavior of l forms of bacteria and mycoplasma in cell cultures]. 19734149907
cultivation of mycoplasmas on cellulose ester substrates.the ability of mycoplasmas to grow on cellulose ester substrates was evaluated. mycoplasma pneumoniae, m. hominis, m. arthritidis, m. gallisepticum, and acholeplasma laidlawii grew on millipore (mixed cellulose ester) filters and sepraphore iii (cellulose polyacetate) membranes.19734584581
selective membrane toxicity of the polyene antibiotics: studies on natural membranes.the effect of polyene antibiotics on candida albicans, human erythrocytes, and acholeplasma laidlawii was studied. the results sustain the observations made with lecithin-sterol liposomes. the distribution of double bonds in the membrane sterol nucleus appears to be of major importance in conferring polyene susceptibility; those sterols with the ergosterol nucleus are far more effective than those with a nucleus similar to cholesterol. different polyenes vary in their membrane selectivity. the c ...19734586145
workshop on the mycoplasmatales as agents of disease. session i. biology of the mycoplasmatales. summary of discussion. 19734570125
genome size and evolution. 19734565043
dilatometry biological membranes. 19734350105
binding of proteins to mycoplasma membranes. 19734354127
mycoplasma-associated induction of interferon in ovine leukocytes.a mycoplasmal species, acholeplasma laidlawii, isolated as a contaminant from a fetal lamb kidney cell line, was shown to be associated with the induction of interferon in cultures of ovine peripheral blood leukocytes. broth cultures of the mycoplasma induced between 20 and 230 u of interferon per ml in leukocytes from two adult ewes. the amount of interferon produced correlated with the inoculum size of mycoplasma. interferon production was associated with replication of the mycoplasma in the l ...19734356073
membrane lipids of mycoplasma hominis.essentially all of the lipids of mycoplasma hominis (200 mug/mg of cell protein) were found to be located in the cell membrane. over one-half were neutral lipids incorporated from the growth medium and consisting of 43% free cholesterol, 19% esterified cholesterol, 23% triglycerides, 10% free fatty acids, and small amounts of di- and monoglycerides. the polar lipids accounting for about 40% of the total were synthesized by the organisms. phosphatidylglycerol was the predominant lipid of this fra ...19734347923
virus-like particles associated with the mycoplasmas of clover phyllody in the plant and in the insect vector. 19734348972
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