Publications
| Title | Abstract | Year(sorted ascending) Filter | PMID Filter |
|---|
| [investigations on the inducible nicotinic acid hydroxylase formation in mycobacterium smegmatis]. | 1970 | 5497301 | |
| chain lengths of the oligodeoxyribonucleotides formed by the nucleoside triphosphate-dependent deoxyribonuclease of mycobacterium smegmatis. | 1970 | 5500292 | |
| utilization of shikimic acid for the formation of mycobactin s and salicylic acid by mycobacterium smegmatis. | 1970 | 5501654 | |
| [evidence of the gene transfer by conjugation in mycobacterium smegmatis]. | 1970 | 5531497 | |
| [genetic recombination between mycobacterium smegmatis (strains jucho and lactocola)]. | 1970 | 5531566 | |
| the positional distribution of fatty acids in the phospholipids and triglycerides of mycobacterium smegmatis and m. bovis bcg. | 1970 | 4916405 | |
| [synchronization of mycobacterium smegmatis culture by phenethyl alcohol and nitrosoguanidine mutagenesis]. | 1970 | 4924836 | |
| [genetic map of mycobacterium smegmatis atcc 607]. | 1970 | 4924838 | |
| uptake of salicylic acid into mycobactin s by growing cells of mycobacterium smegmatis. | 1970 | 11945420 | |
| utilization of amino acids during growth of mycobacterium tuberculosis in rotary cultures. | marked differences were observed in the response of actively growing cells of the saprophyte, mycobacterium smegmatis 607, and the avirulent human strain, m. tuberculosis (h37ra), to several different nitrogen sources in aerated (rotary) cultures. the growth-promoting effect and utilization of equimolar concentrations (5 mumoles/ml) of l-alanine, l-aspartic acid, monosodium glutamate, or ammonium chloride were compared with that of l-asparagine, the normal nitrogen source, in sauton synthetic li ... | 1970 | 16557769 |
| polysaccharides of mycobacterium bovis ushi 10, mycobacterium smegmatis, mycobacterium phlei, and atypical mycobacterium p1. | four kinds of polysaccharides, glucan, mannan, arabinomannan, and arabinogalactan, were purified from the defatted cells of four strains of mycobacteria. by the chemical and immunological analyses, we concluded that arabinomannan and arabinogalactan are common serologically active polysaccharides in mycobacteria. | 1970 | 16557844 |
| protection of mycobacterium smegmatis from ethambutol and streptomycin inhibition by mgso(4) and polyamines. | polyamines protected mycobacterium smegmatis from ethambutol inhibition but not from streptomycin inhibition, whereas mgso(4) neutralized growth inhibition by both drugs. | 1971 | 16558002 |
| mannolipid synthesis in a cell-free system of mycobacterium smegmatis. | 1971 | 11946084 | |
| comparison of the effects of carbon, nitrogen and iron limitation on the growth and on the rna and dna content of mycobacterium smegmatis. | 1971 | 4925435 | |
| influence of metal ions on the formation of mycobactin and salicylic acid in mycobacterium smegmatis grown in static culture. | mycobacterium smegmatis was grown on trace-metal-free medium in static culture. throughout the growth phase, the concentration of mycobactin increased continuously, reaching a maximum of about 30 to 40 mug of mycobactin/mg of cell dry weight after 6 days; the concentration of salicylic acid remained approximately constant at 1 to 2 mug of salicylic acid/mug of cell dry weight. fe(2+) (or fe(3+)), zn(2+), mn(2+), and mg(2+) were all essential to a maximum formation of mycobactin. optimum concentr ... | 1971 | 4941561 |
| mechanism of action of rifampin on mycobacterium smegmatis. | deoxyribonucleic acid (dna)-dependent ribonucleic acid (rna) polymerase (ec 2.7.7.6) isolated from a rifampin-sensitive strain of mycobacterium smegmatis was 90% inhibited by 1 mug of rifampin per ml; enzyme from a rifampin-resistant mutant was not affected by this concentration of antibiotic. inhibition of phenylalanine-1-(14)c incorporation by rifampin in growing cultures was complete about 6 min after addition of antibiotic. under the same conditions, uracil-2-(14)c incorporated was blocked a ... | 1971 | 4942761 |
| transport of iron by mycobactin in mycobacterium smegmatis. | 1971 | 5117557 | |
| partial purification and properties of a dna polymerase from mycobacterium smegmatis. | 1971 | 5136449 | |
| partial purification and properties of a nucleoside triphosphate-dependent deoxyribonuclease from mycobacterium smegmatis. | 1971 | 5141665 | |
| formation of polydeoxyadenylylthmidylate after deoxyribonucleic acid synthesis by a deoxyribonucleic acid polymerase from mycobacterium smegmatis. | 1971 | 5144215 | |
| the experimental production of mastitis in sheep by mycobacterium smegmatis and mycobacterium fortuitum. | 1971 | 5166136 | |
| a procedure for selecting and isolating specific auxotrophic mutants of mycobacterium smegmatis. | 1971 | 5559612 | |
| nature of the nuclease activity prevalent in mycobacterium smegmatis during in vitro amino acid incorporation. | 1971 | 4324242 | |
| conversion of chorismic acid and isochorismic acid to salicylic acid by cell-free extracts of mycobacterium smegmatis. | 1971 | 4325683 | |
| partial purification and properties of a highly specific trehalose phosphate phosphatase from mycobacterium smegmatis. | a specific trehalose phosphate phosphatase was purified approximately 50-fold from mycobacterium smegmatis. the enzyme had a ph optimum of about 7.0 and was stimulated by mg(2+). the optimum concentration of mg(2+) was about 1.5 x 10(-3)m. of other divalent cations tested, only co(2+) showed some activity. the k(m) for trehalose phosphate was found to be about 1.5 x 10(-3)m. the enzyme showed slight activity toward mannose-6-p and fructose-6-p but was inactive on a large number of other phosphor ... | 1971 | 4327508 |
| recombination between mycobacterium smegmatis strains jucho and lacticola. | 1971 | 5315708 | |
| aspartic acid transport in mycobacterium smegmatis. | 1971 | 5316573 | |
| [mode of action of ionic surface-active compounds in bacteria. ii. electron microscopic investigations on the influence of sodium lauryl sulfate (nals) on mycobacterium smegmatis]. | 1971 | 4105096 | |
| mycosides c: behavior as receptor site substance for mycobacteriophage d4. | interpretation of an earlier published infrared spectrum of mycobacterium smegmatis lipids with receptor site activity for d4 phage led us to the inference that the active substance is very likely a mycoside c. this hypothesis was confirmed: the well-characterized mycosides c(s) and c(1217) elaborated by the heterologous strains m. scrofulaceum and mycobacterium species 1217, respectively, are essentially indistinguishable from the smegmatis lipids in their behavior toward d4. minute quantities ... | 1972 | 4113889 |
| properties of polyphosphate kinase prepared from mycobacterium smegmatis. | 1972 | 4337332 | |
| analysis of ribosomes from viomycin-sensitive and -resistant strains of mycobacterium smegmatis. | viomycin-resistant strains were isolated from mycobacterium smegmatis. ribosomes were isolated and tested for drug resistance in subcellular systems containing poly(u) as messenger ribonucleic acid. resistance to viomycin in these strains was due to altered ribosomes. further analysis showed that viomycin resistance of two mutants with low level resistance (20 mug/ml) was due to altered 30s ribosomal subunits. another mutant that was highly resistant to viomycin (1 mg/ml), however, had altered 5 ... | 1972 | 4342812 |
| isolation of a particulate (membrane) fraction from mycobacterium smegmatis showing phosphorylation coupled to malate oxidation. | 1972 | 4404431 | |
| isolation and properties of a macromolecular, water-soluble, immuno-adjuvant fraction from the cell wall of mycobacterium smegmatis. | trypsin- and chymotrypsin-treated delipidated cell walls of mycobacterium smegmatis were digested overnight with lysozyme. the water-soluble products thus obtained were filtered on a column of sephadex g-50; the first peak, excluded from the column, has immunological adjuvant activity. the material of the excluded peak is obtained after lyophilization as a snow-white, fluffy material, soluble in water and insoluble in organic solvents. it behaves as a slightly polydisperse macromolecule in an ul ... | 1972 | 4502937 |
| a presumptive test for the detection of mycobacterium smegmatis in milk. | 1972 | 4552940 | |
| relationships between oleic acid uptake and lipid metabolism in mycobacterium smegmatis. | 1972 | 4562799 | |
| salicylic acid biosynthesis and its control in mycobacterium smegmatis. | 1972 | 4623285 | |
| iron transport in mycobacterium smegmatis: the role of mycobactin. | 1972 | 4652567 | |
| turnover of acylglucose, acyltrehalose and free trehalose during growth of mycobacterium smegmatis on glucose. | 1972 | 4657139 | |
| structure of a transducing mycobacteriophage. | electron micrographs of the transducing phage i3 for mycobacterium smegmatis strain sn2 revealed a phage with a contractile tail and a head with isometric symmetry and visible capsomeres. | 1972 | 5014933 |
| the phospholipids of mycobacterium smegmatis. | 1972 | 5017887 | |
| isoniazid metabolism and binding by sensitive and resistant strains of mycobacterium smegmatis. | 1972 | 5034223 | |
| preliminary observations on the occurrence of a streptomycin r-factor in mycobacterium smegmatis atcc 607. | 1972 | 5038198 | |
| antibiotics from higher plants. thalictrum rugosum. thalrugosamine, a new bisbenzylisoquinoline alkaloid active vs mycobacterium smegmatis. | 1972 | 5040786 | |
| nature of the receptor substance of mycobacterium smegmatis for d4 bacteriophage adsorption. | the acetone-soluble fraction extracted from lyophilized cells of mycobacterium smegmatis atcc 607 inhibited d4, a species-specific phage active against m. smegmatis. evidence is presented indicating that the d4 inhibition was caused by the phage receptor substance(s) contained in this fraction. a fraction eluted from silicic acid with chloroform-methanol (95:5, v/v) showed the strongest inhibition of d4 phage. this fraction contained sugars and amino acids, and its infrared absorption spectrum w ... | 1972 | 5053464 |
| isolation and properties of auxotrophic mutants of mycobacterium smegmatis requiring either salicylic acid or mycobactin. | 1972 | 5071388 | |
| inhibition of mycobactin formation in mycobacterium smegmatis by p-aminosalicylate. a new proposal for the mode of action of p-aminosalicylate. | 1972 | 5078877 | |
| purification and properties of the adenosine diphosphate-glucose and uridine diphosphate-glucose pyrophosphorylases of mycobacterium smegmatis: inhibition and activation of the adenosine diphosphate-glucose pyrophosphorylase. | crude extracts of mycobacterium smegmatis catalyzed the synthesis of adenosine diphosphate-glucose (adp-glc), cytidine diphosphate-glucose, guanosine diphosphate-glucose (gdp-glc), thymidine diphosphate-glucose (tdp-glc), and uridine diphosphate-glucose (udp-glc). in these crude enzyme fractions, high concentrations of trehalose-p inhibited the adp-glc and gdp-glc pyrophosphorylases but did not effect the udp-glc or tdp-glc pyrophosphorylases. both the adp-glc pyrophosphorylase and the udp-glc p ... | 1972 | 5079067 |
| ethambutol-mediated alterations in ribonucleic acid components of mycobacterium smegmatis. | polyacrylamide gel electrophoresis of ribonucleic acid (rna) components from mycobacterium smegmatis exposed to ethambutol shows that the early effect of the drug produces a selective alteration of some rna species, suggestive of a noncoordinate control of rna metabolism. | 1972 | 5086655 |
| relationships among mycobacteria and nocardiae based upon deoxyribonucleic acid reassociation. | the degree of renaturation between nocardia farcinica 330 deoxyribonucleic acid (dna) and dna from 17 nocardial strains and 11 mycobacterial strains, and between mycobacterium smegmatis 405 dna and dna from 11 mycobacterial strains and 14 nocardial strains was determined by using the nitrocellulose membrane filter technique. these results indicated that some cultures designated n. farcinica were identical to some cultures designated n. asteroides but that other strains called n. asteroides were ... | 1973 | 4690961 |
| genetic recombination in mycobacteria. | evidence for genetic recombination between mycobacterium smegmatis strain rabinowitchi (rab) and strain jucho or pm5 is presented. backcrosses of recombinants by either parental strain indicated four different types of mating behavior, suggesting that the mycobacterial compatibilities are controlled by at least two different factors. no sex factor that transfers at a high frequency or that is sensitive to acridine dyes was detected. analysis of segregation of unselected markers revealed that str ... | 1973 | 4691386 |
| preparation and biological properties of water-soluble adjuvant fractions from delipidated cells of mycobacterium smegmatis and nocardia opaca. | digestion by lysozyme of delipidated cells of mycobacterium smegmatis liberates a water-soluble immunoadjuvant fraction which is chemically very similar to the water-soluble adjuvant (wsa) obtained previously from purified cell walls, but which contains somewhat more non-peptidoglycan amino acids. the yield of peptidoglycan-arabinogalactan complex is about 10 times greater starting from whole cells than from cell walls. the main biological properties of this "neo-wsa" are described: it increases ... | 1973 | 4716544 |
| effects of hydroxyurea, nalidixic acid and zinc limitation on dna polymerase and atp-dependent deoxyribonuclease activities of mycobacterium smegmatis. | 1973 | 4723068 | |
| primary binding of ethambutol by mycobacterium smegmatis. | 1973 | 4741892 | |
| characterization of the alkali-stable mannophospholipids of mycobacterium smegmatis. | 1973 | 4741910 | |
| uptake of ethambutol by mycobacterium smegmatis and its relation to growth inhibition. | 1973 | 4745267 | |
| [palmity-coa-acp-transacylase and malonyl-coa-acp-transacylase of mycobacterium smegmatis]. | 1973 | 4749726 | |
| acyl-coa-acp-transacylases of mycobacterium smegmatis. an analytical study. | 1973 | 4790850 | |
| nonspecific ionic inhibition of ethambutol binding by mycobacterium smegmatis. | magnesium sulfate and spermidine were tested for their effects on binding of (14)c-ethambutol by mycobacterium smegmatis. concentrations were used that protected the organism from ethambutol inhibition. sodium salts were examined as possible ethambutol antagonists to test the previously reported specificity of the divalent cation salt effect. consistent with growth-protection experiments, 20 mm mgso(4) or 2.0 mm spermidine prevented and reversed (14)c binding by cells shaken with 0.2 mug of (14) ... | 1973 | 4790932 |
| transduction of isoniazid susceptibility-resistance and streptomycin resistance in mycobacteria. | transduction of resistance to isoniazid and streptomycin as well as susceptibility to isoniazid in mycobacterium smegmatis sn2 has been demonstrated. a method has been described for the selection of isoniazid-susceptible variants after transduction of susceptibility. | 1973 | 4793884 |
| levels of glycogen and trehalose in mycobacterium smegmatis and the purification and properties of the glycogen synthetase. | the levels of glycogen, free trehalose, and lipid-bound trehalose were compared in mycobacterium smegmatis grown under various conditions of nitrogen limitation. in a mineral salts medium supplemented with yeast extract and containing fructose as the carbon source, the accumulation of glycogen increased dramatically as the nh(4)cl content of the medium was lowered. however, levels of free trehalose remained relatively constant. cells were grown in low nitrogen medium and were then shifted to med ... | 1973 | 4632324 |
| [antigenic analyses and cross reactions between leishmaniae, trypanosoma cruzi and mycobacterium smegmatis (author's transl)]. | 1973 | 4200672 | |
| [complete separation of palmityl-coa-acp-transacylase activity and malonyl-coa-acp-transacylase activity in homogenates of mycobacterium smegmatis]. | 1973 | 4204039 | |
| transduction of a streptomycin r-factor from mycobacterium smegmatis to mycobacterium tuberculosis h37rv. | 1974 | 4220076 | |
| [use of a water-soluble adjuvant fraction from "mycobacterium smegmatis" with sperm extract to induce autoimmune aspermatogenic orchiepididymitis in guniea pigs (author's transl)]. | 1974 | 4282838 | |
| iron transport in mycobacterium smegmatis: a restricted role for salicylic acid in the extracellular environment. | 1974 | 4425672 | |
| effects of polyanions and polycations on the trehalose phosphate synthetase of mycobacterium smegmatis. | 1974 | 4429969 | |
| biosynthesis of glycosyldiglycerides in mycobacterium smegmatis. | a particulate enzyme preparation from mycobacterium smegmatis catalyzes the transfer of [(14)c]galactose from uridine 5'-diphosphate (udp)-[(14)c]galactose and of [(14)c]glucose from udp-[(14)c]glucose into chloroform-soluble products. the radioactive neutral lipids were purified by passage through diethylaminoethyl-cellulose, followed by thin-layer chromatography. when udp-glucose was used as substrate, two major radioactive lipids were obtained; one had a hexose-glucose-glycerol ratio of 1:1:1 ... | 1974 | 4808895 |
| effect of ultraviolet-sensitive mutants on gene inheritance in mycobacterial matings. | evidence is presented suggesting that a rec mutation in one strain (presumably female) of a mating pair of mycobacterium smegmatis strains prevented the appearance of recombinants, whereas a rec mutation in the other strain (presumably male) did not. | 1974 | 4811552 |
| delayed hypersensitivity reactions provoked by ribosomes from acid-fast bacilli: physical characteristics and immunological aspects of core ribosomal proteins from mycobacterium smegmatis. | ribosomal subunits from mycobacterium smegmatis were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. these s(20.w) values for the subunits are 48.7s and 28.1s. the molecular weight of the 49s subunit is about 1.65 x 10(6), and that of the 28s subunit is 7.8 x 10(5). both subunits contain about 37% protein and 63% ribonucleic acid. a protein-deficient particle having an s(20.w) value of 15.7s contains about 11% protein and 89% r ... | 1974 | 4816630 |
| biosynthesis of mannosyl--and glucosyl-phosphoryl polyprenols in mycobacterium smegmatis. evidence for oligosaccharide-phosphoryl-polyprenols. | 1974 | 4828528 | |
| chemical characterization of ethambutol binding to mycobacterium smegmatis. | recent studies showed that critical binding of ethambutol to mycobacterium smegmatis was both inhibited and reversed by ions. this apparent ion-susceptible characteristic suggested that ethambutol is held at critical sites by electrostatic bonds. dissociation constant, ph, and ionic strength studies were designed to further characterize ethambutol binding. m. smegmatis was grown in sauton synthetic liquid medium (ph 7.4) under aerated conditions at 37 c. the ph or ionic strength of the medium wa ... | 1974 | 4840434 |
| binding of dihydrostreptomycin to ribosomes from viomycin-sensitive and -resistant strains of mycobacterium smegmatis. | 1974 | 4136561 | |
| mapping of antibiotic resistance markers in mycobacterium smegmatis. | 1974 | 4139295 | |
| genetic and biochemical studies on drug-resistant mutants in mycobacterium smegmatis. | 1974 | 4141739 | |
| [use of water-soluble adjuvant fraction from "mycobacterium smegmatis" with encephalitogenic basic protein to induce autoimmune experimental allergic encephalomyelitis in guinea pigs (author's transl)]. | 1974 | 4143099 | |
| inhibition of growth of mycobacterium smegmatis and of cell wall synthesis by d-serine. | d-serine inhibited the growth of mycobacterium smegmatis and induced the morphological alteration of the bacilli. the growth inhibitory action of d-serine was partially reduced by an equimolecular concentration of d-alanine. the combination of glycine with d-alanine reversed the growth inhibition produced by d-serine more than did d-alanine alone. in cells cultured in the presence of d-serine, the amounts of alanine, diaminopimelic acid, and glycine inserted into the cell wall mucopeptide were r ... | 1974 | 15828163 |
| pleiotropic antibiotic resistance mutations associated with ribosomes and ribosomal subunits in mycobacterium smegmatis. | viomycin-resistant strains isolated from mycobacterium smegmatis demonstrated pleiotropic resistance to tuberactinomycin-n, capreomycin, streptomycin, and kanamycin as a result of mutational alteration of ribosomes, even though they were selected for resistance to a single antibiotic. the pleiotropic drug resistance of three mutants isolated by stepwise selection for resistance to viomycin was due to alteration of the 30s ribosomal subunit. one mutant, strain a, isolated independently by multipl ... | 1974 | 15828170 |
| inhibition of dihydrostreptomycin action on mycobacterium smegmatis by monovalent and divalent cation salts. | we have examined and compared the effects of monovalent and divalent cation salts on dihydrostreptomycin (dsm) action against mycobacterium smegmatis. the sauton synthetic liquid medium used was supplemented with test salts on the basis of ionic strength (mu). turbidimetric growth experiments showed that 0.02 m mgso(4) (mu = 0.08) prevented growth inhibition by 0.1 mug of dihydrostreptomycin per ml, but 0.02 m nacl (mu = 0.02) did not. however, at molarities equivalent to mu = 0.08, four monoval ... | 1975 | 50048 |
| enhancement of immunity against murine syngeneic tumors by a fraction extracted from non-pathogenic mycobacteria. | the data reported here demonstrate that a preparation extracted from nonpathogenic mycobacteria such as mycobacterium smegmatis and hereafter referred to as interphase material protected mice against ehrlich ascitic carcinoma, l-1210 leukemia, and another syngeneic lymphoid leukemia. furthermore, mice treated by this preparation were much less susceptible to endotoxins than when stimulated by bcg (bacillus calmette-guerin) or m. smegmatis cells. moreover, guinea pigs treated by interphase materi ... | 1975 | 171670 |
| variations in the pathways of malate oxidation and phosphorylation in different species of mycobacteria. | mycobacterium tuberculosis h37rv, the slow-growing human pathogenic strain of tubercle bacilli and mycobacterium smegmatis and mycobacterium phlei, the fast-growing saprophytes, have shown variations regarding the type of dehydrogenase that initiates malate oxidation in the respiratory chain. m. tuberculosis h37rv is characterized by having a malate oxidase system (designated malnad pathway) in which malate oxidation is mediated by the nad+-dependent malate dehydrogenase (ec 1.1.1.37) but not by ... | 1975 | 234747 |
| iron transport in mycobacterium smegmatis: ferrimycobactin reductase (nad(p)h:ferrimycobactin oxidoreductase), the enzyme releasing iron from its carrier. | 1975 | 237787 | |
| purification, crystallization, and properties of d-ribose isomerase from mycobacterium smegmatis. | d-ribose isomerase, which catalyzes the conversion of d-ribose to d-ribulose, was purified from extracts of mycobacterium smegmatis grown on d-ribose. the purified enzyme crystalized as hexagonal plates from a 44% solution of ammonium sulfate. the enzyme was homogenous by disc gel electrophoresis and ultracentrifugal analysis. the molecular weight of the enzyme was between 145,000 and 174,000 by sedimentation equilibrium analysis. its sedimentation constant of 8.7 s indicates it is globular. on ... | 1975 | 240851 |
| the effect of leukocyte hydrolases on bacteria. iii. bacteriolysis induced by extracts of different leukocyte populations and the inhibition of lysis by macromolecular substances. | the lysis of 14c-labeled bacteria by hydrolases of human and rabbit leukocytes was studied in vitro. while staphylococcus albus, streptococcus faecalis, and streptococcus mutans were highly susceptible to lysis, staphylococcus auresus was intermediate in its susecptibility to lysis by the leukocyte enzymes. group a streptococcus, listeria monocytogenes, shigella flexneri, escherichia coli, and mycobacterium smegmatis were very resistant to degradation by these enzymes. the lytic activity of leuk ... | 1975 | 804017 |
| biologically active components from mycobacterial cell walls. iii. production of experimental allergic encephalomyelitis in guinea-pigs. | the efficacy of various fractions of mycobacterial cell walls in producing experimental ahlergic encephalomyelitis (eae) has been evaluated. bcg (bacillus-calmette-buérin) cell walls were effective in producing eae in all animals at dose levels as low as 40 mug. study of subfractions of these cell walls revealed the following: (1) wax d was active, but required larger doses than bcg cell walls; (2) the chloroform-methanol-soluble (cms) portion of wax d and p3 (a mycolic acid-trehalose ester cont ... | 1975 | 804436 |
| biological studies of amiclenomycin. | the action of amiclenomycin (am) in inhibiting growth of microorganisms is specific against mycobacteria in vitro, but the antibiotic does not show a therapeutic effect against tubercle bacilli in vivo. the action of am is reversed by biotin, desthiobiotin (dtb) and 7,8-diaminopelargonic acid (dapa), but not by 7-keto-8-aminopelargonic acid (kapa), pimelic acid and glutaric acid. in the presence of am, cultures of mycobacterium smegmatis and bacillus sphaericus accumulated kapa, whereas the form ... | 1975 | 805118 |
| two antimicrobial alkaloids from heartwood of liriodendron tulipifera l. | alcoholic extracts of the heartwood of liriodendron tulipifera have demonstrated antimicrobial activity against staphylococcus aureus, mycobacterium smegmatis, candida albicans, and aspergillus niger. the antimicrobial activity was associated only with the alkaloidal fraction. separation of the active alkaloidal fraction by chromatography led to the isolation and identification of dehydroglaucine and liriodenine as the active components. several other alkaloidal derivatives were prepared and tes ... | 1975 | 807704 |
| studies on the mechanism of mycobacterium smegmatis l-lactate oxidase. 5-deazaflavin mononucleotide as a coenzyme analogue. | apo-lactic oxidase from mycobacterium smegmatis reconstituted with the deazaisoalloxazine analogue of fmn, 5-deazafmn, undergoes reduction by l-lactate but not catalytic reoxidation by o2. the rate of deazafmn-holo-enzyme reduction by substrate is 1.1 min-1. with l-[alpha-3-h]-lactate, direct tritium transfer to enzyme-bound deazafmn occurs during reduction. no evidence for a stable covalent lactate-deazafmn adduct has been obtained. the deaza-fmnh2-enzyme is reoxidized extremely slowly by o2, c ... | 1975 | 234460 |
| the effect of p-aminosalicyclic acid on iron transport and assimilation in mycobacteria. | p-aminosalicylic acid inhibits growth of mycobacterium bovis bcg and mycobacterium smegmatis more effectively if cells are growing with a sufficiency of iron (more than 1 mu g fe/ml) in the medium than if cells are deficient in iron (smaller than 0.1 mu g fe/ml). in iron-deficient cultures formation of mycobactin, an ionophore for iron transport, is strongly inhibited by p-aminosalicylic acid. uptake of iron into cell suspensions is also inhibited and the activity of several iron-containing enzy ... | 1975 | 1092357 |
| [the influence of mycobacterium bovis bcg-prague, respectively of mycobacterium smegmatis on the growth of the intratracheally transplanted deal's sarcoma in guinea pigs (author's transl)]. | 1975 | 1098744 | |
| the influence of mycobacterium bovis bcg-prague, respectively of mycobacterium smegmatis on the growth of the intratracheally transplanted deal's sarcoma in guinea pigs (author's transl)]. | 1975 | 1100201 | |
| possibilities of the conjugation process in mycobacteria. | results obtained when studying conjugation in mycobacteria by means of different methods are summarized. the method of conjugation on surface of a solid complete medium was tested with different auxotrophic mutants of different strains of mycobacterium smegmatis. it was not possible to obtain positive results even by means of the above method. this was probably due to unsuitability of the chosen strains of mycobacterium smegmatis. preparation of the donor strain by transfer of the f factor from ... | 1975 | 1104423 |
| further analysis of acyl-coa-acp-transacylases of mycobacterium smegmatis. identification of a long chain alkyl malonyl-coa-acp-transacylase. | homogenates were prepared from three sources, mycobacterium smegmatis saccharomyces cerevisiae and escherichia coli and tested for docosyl malonyl-coa-acp transacylase activity, using acp purified from e. coli strain b and [2r, 2s, 1, 3-14c2] docosyl malonyl-coa synthesized chemically, as substrates. only homogenates of m. semegmatis showed positive transacylase activity. successive chromatographies on sephadex g-150 and then on deae-sephadex a-50 prove that neither the palmityl-coa-acp-transacy ... | 1975 | 1106774 |
| extracellular iron acquisition by mycobacteria: role of the exochelins and evidence against the participation of mycobactin. | mycobacterium bovis var. bcg was grown under iron-deficient conditions in the presence and absence of 1% tween 80. mycobactin, the iron iron ionophore of mycobacteria, was found solely within the bacteria grown in the absence of tween, but low concentrations (0.75 mug/ml) of it appeared in the medium in the presence of the surfactant. both types of medium contain agents, named exochelins, which could solubilize iron. 55fe added to spent culture media was recovered only chelated to these compound ... | 1975 | 1107222 |
| the role of mannosylphosphorylpolyisoprenol in glycoprotein biosynthesis in mycobacterium smegmatis. | a particulate enzyme preparation of mycobacterium smegmatis catalyzes the transfer of labeled mannose from gdp-[14c] mannose into several endogenous acceptors. in one of these transfer reactions, the radioactivity is incorporated into an insoluble polymeric product that is present at the interphase after extraction of reaction mixture with chloroform-methanol. solubilization of this product was achieved by digestion with proteolytic enzymes or treatment with 0.1 m naoh indicating that the materi ... | 1975 | 1111583 |
| complex formation between mycobacterial polysaccharides or cyclodextrins and palmitoyl coenzyme a. | the mycobacterial polysaccharides mmp (3-o-methyl-mannose-containing polysaccharide), mglp (lipolysaccharide containing 6-o-methylglucose and glucose), and the cyclodextrins (cyclohexaamylose and cycloheptaamylose) form stoichiometric complexes with palmitoyl-coa (machida, y., bergeron, r., flick, p., and bloch, k. (1973) j. biol. chem. 248, 6246-6247). complex formation is presumed to result from hydrophobic interactions. in order to enhance the hydrophobic character of the cyclodextrins the fo ... | 1975 | 1112801 |
| reversible inhibition of the fatty acid synthetase complex from mycobacterium smegmatis by palmitoyl-coenzyme a. | palmitoyl-coa dissociates the fatty acid synthetase complex from mycobacterium smegmatis into inactive subunits of molecular weight 250,000 as determined by sucrose density gradient centrifugation. palmitoyl-coa binds to the subunits but the binding can be prevented and reversed by the mycobacterial 3-o-methylmannose-containing polysaccharide. when the palmitoyl-coa containing inactive subunits were isolated by gel filtration on sepharose 6b, and then concentrated and dialyzed against 0.5 m phos ... | 1975 | 1123344 |
| nonspecificity in the divalent cation antagonism of dihydrostreptomycin action on mycobacterium smegmatis. | a divalent cation-dependent antagonism of dihydrostreptomycin action on mycobacterium smegmatis was demonstrated. however, the nutritionally nonessential cations sr++ and ba++ inhibited drug action as effectively as the essential ions mg++ and ca++, indicating a high degree of nonspecificity. | 1975 | 1124316 |
| antimicrobial agents from marine algae. | the antimicrobial activity of five compounds extracted from marine algae was tested against staphylococcus aureus, salmonella choleraesuis, mycobacterium smegmatis, candida albicans, and escherichia coli. three of the compounds, cycloeudesmol, laurinterol, and debromolaurinterol, exhibited activity at concentrations approaching that of streptomycin. none of the compounds inhibited all of the organisms tested. there appeared to be selectivity for gram-positive microbes. | 1975 | 1137385 |
| fructose diphosphate aldolase from mycobacterium smegmatis. purification and properties. | 1975 | 1137395 | |
| fructose diphosphate aldolase from mycobacterium smegmatis. functional similarities with rabbit muscle aldolase. | 1975 | 1137396 |