Publications

purification and partial characterization of a novel antibacterial agent (bac1829) produced by staphylococcus aureus ksi1829.	a novel antimicrobial agent from staphylococcus aureus ksi1829, designated bac1829, was purified by sequential steps of ammonium sulfate precipitation, sephadex g-50 gel filtration chromatography, and hydrophobic interaction chromatography. purified bac1829 has a molecular mass of 6,418 +/- 2 da. the peptide in heat stable, since full biological activity is retained after heating at 95 degrees c for 15 min, and it is destroyed by digestion with proteases. amino acid sequence analysis revealed a ...	1996	8795206
[an experimental study of infection stone formation by corynebacterium species].	the relationship between infection stone and corynebacterium species was investigated in vitro and in vivo. urease activity of urease-splitting corynebacterium species was evaluated by 2 methods; an increase in ph of human urine after inoculation of corynebacterium species and direct measurement of urease activity of 10(7) cfu organisms from amounts of ammonia by indophenol method. formation of infection bladder stone was induced in male wistar rats by implanting a zinc disc and inoculating 10(6 ...	1997	9086346
purification and characterization of staphylococcin bacr1, a broad-spectrum bacteriocin.	the bacteriocin bacr1 was purified from culture supernatant of staphylococcus aureus ut0007 by sequential ammonium sulfate precipitation, cation-exchange chromatography, and c4 reverse-phase chromatography steps. mass spectrographic analysis indicated that the purified peptide has a molecular mass of 3,338 da. it is resistant to environmental conditions, retaining full biological activity after exposure to ph extremes (phs 3 to 11), heating at 95 degrees c for 15 min, and exposure to strong chao ...	1997	9361402
toxigenic strains of bacillus licheniformis related to food poisoning.	toxin-producing isolates of bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. the toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic toxin of bacillus cereus, cereulide. cell extracts of the toxigenic b. licheniformis isolates inhibited sperm motility, damaged cell membrane integrity, depleted cellular atp, and s ...	1999	10508100
corynebacterium renale infection in calves.		2000	10674699
isolation of toxigenic nocardiopsis strains from indoor environments and description of two new nocardiopsis species, n. exhalans sp. nov. and n. umidischolae sp. nov.	nocardiopsis strains were isolated from water-damaged indoor environments. two strains (n. alba subsp. alba 704a and a strain representing a novel species, es10.1) as well as strains of n. prasina, n. lucentensis, and n. tropica produced methanol-soluble toxins that paralyzed the motility of boar spermatozoa at <30 microg of crude extract (dry weight) x ml(-1). n. prasina, n. lucentensis, n. tropica, and strain es10.1 caused cessation of motility by dissipating the mitochondrial membrane potenti ...	2001	11526036
biofilm bacteria: formation and comparative susceptibility to antibiotics.	the calgary biofilm device (cbd) was used to form bacterial biofilms of selected veterinary gram-negative and gram-positive pathogenic bacteria from cattle, sheep, pigs, chicken, and turkeys. the minimum inhibitory concentration (mic) and minimum biofilm eradication concentration (mbec) of ampicillin, ceftiofur, cloxacillin, oxytetracycline, penicillin g, streptomycin, tetracycline, enrofloxacin, erythromycin, gentamicin, tilmicosin, and trimethoprim-sulfadoxine for gram-positive and -negative b ...	2002	11989739
four-year survey of urinary tract infections in calves in israel.	the prevalence of urinary tract infections in calves aged seven days to three months in three dairy cattle herds ranged from 0.5 to 1.6 per cent, with an average of 1.1 per cent. the mortality rate reached 16.1 per cent. the morbidity rate of the female calves was 1.4 per cent and that of the male calves 0.8 per cent. the bacteria isolated from urine, and from vaginal and preputial swabs were escherichia coli (35 per cent), corynebacterium renale (14 per cent), plasma coagulase-negative staphylo ...	2004	14994858
cystoscopy in cattle--a valuable additional tool for clinical examination.	based on the findings of physical examination and on laboratory findings the urinary bladder of 23 cows was examined endoscopically in order to investigate the application of cystoscopy in cattle. the endoscopic findings of all examined cows were compared with the findings of physical examination and the results of macroscopic and microscopic urinalysis and the bacteriological culture of the urine. by physical examination only 3 cows were diagnosed to have urinary tract disease, whereas all othe ...	2004	15595276
high prevalence of ulcerative posthitis in rasa aragonesa rams associated with a legume-rich diet.	this study describes an outbreak of ulcerative posthitis that eventually affected 76 of 80 of rams in a flock of rasa aragonesa sheep on a legume-rich diet. lesions were confined to the prepuce and varied from mild hyperaemia to ulcerations. corynebacterium renale was isolated from the lesions. treatment of an initial group of 17 was based on a change of diet and topical treatment with veterin banedif with prednisolone once a day for 15 days and was successful. lesions in this group were complet ...	2005	15882402
slaughterhouse survey of pyelonephritis in dairy cows.	twenty-one rejected kidneys from 2426 slaughtered dairy cows (0.87 per cent) had gross signs of pyelonephritis that were confirmed by histopathology. in all the kidneys the findings were consistent with a chronic rather than an acute infection. one species of bacteria was cultured from 12 of the kidneys and two species of bacteria were cultured from six. the most commonly isolated bacteria were escherichia coli, from eight kidneys, arcanobacterium pyogenes, from seven kidneys and corynebacterium ...	2005	16299366
a herd level analysis of urinary tract infection in dairy cattle.	cystitis, urethritis and pyelonephritis in cattle most commonly result from ascending urinary tract infection with corynebacterium renale, corynebacterium cystidis, corynebacterium pilosum or escherichia coli. we describe the clinical, bacteriological, clinical-pathological and epidemiological findings in a dairy cattle herd with urinary tract infection (uti). blood and urine samples from 17 calves and 19 cows were submitted to laboratory examinations. depression, muscle wasting, weakness and fr ...	2006	16310382
characterization of broad host range cryptic plasmid pcr1 from corynebacterium renale.	plasmid pcr1 is a cryptic plasmid harboured by corynebacterium renale. it is the smallest corynebacterial plasmid known to date. although its natural host is animal corynebacteria, it can replicate in several strains of soil corynebacteria. it can also replicate in escherichia coli, in which it is stably maintained. the copy number of pcr1 in this host is higher than that of puc19, with which it shows unidirectional incompatibility. it is also incompatible with pbk2, a plasmid bearing the common ...	2006	16545871
sonographic application in the diagnosis of pyelonephritis in cattle.	nineteen cattle with pyelonephritis were examined sonographically. a 3.5- and 5.0-mhz convex transducer was used for percutaneous and a 5.5-mhz linear scanner for transrectal examination. ultrasonographic changes included enlarged kidneys, echogenic material, and flocculent fluid within a dilated and deformed renal sinus and a poor cortical medullary differentiation. azotemia was detected in 10 and hematuria and proteinuria in all animals. microbial culture revealed corynebacterium renale in eig ...	2007	17236364
development of expression vectors for escherichia coli based on the pcr2 replicon.	recent developments in metabolic engineering and the need for expanded compatibility required for co-expression studies, underscore the importance of developing new plasmid vectors with properties such as stability and compatibility.	2007	17490494
corynebacterial necrohemorrhagic cystitis in two female macaques.	we describe severe necrohemorrhagic cystitis in a female rhesus macaque and a female cynomolgus macaque due to colonization of the urinary bladder by corynebacterium sp. clinically, both macaques presented with perineal bleeding and depression and, despite extensive and prolonged treatment, succumbed to the disease. at necropsy, the contents of the urinary bladders in both cases were hemorrhagic to greenish black, and the bladder mucosa was necrotic. the major microscopic finding in each case wa ...	2007	17994677
corynebacterium pseudotuberculosis and corynebacterium renale isolated from two arabian oryx (oryx leucoryx).		2008	18587065
clinical and ultrasonographic findings, diagnosis and treatment of pyelonephritis in 17 cows.	the goal of the present study was to describe the clinical, haematological and ultrasonographic findings and treatment of 17 cattle with pyelonephritis. fifteen cattle had an abnormal general condition, which varied in severity; five animals had signs of colic. the urine was brownish-red in 11 animals and cloudy in 13. clumps of purulent material were seen in the urine of nine animals and clots of blood in two. the specific gravity was lower than normal in 13 animals and ranged from 1.005 to 1.0 ...	2008	17363303
composition and characteristics of urinary calculi from guinea pigs.	to determine the mineral composition of calculi, anatomic locations of the calculi, and findings of urinalysis and bacteriologic culture of urine and calculi in guinea pigs with urolithiasis.	2009	19210239
corynebacterium timonense sp. nov. and corynebacterium massiliense sp. nov., isolated from human blood and human articular hip fluid.	gram-positive, facultatively anaerobic, rod-shaped bacteria were isolated from the blood of a patient with endocarditis (strain 5401744t) and from the hip joint fluid of a patient with an infected orthopaedic prosthesis (strain 5402485t). these strains were characterized by using a polyphasic taxonomic approach. based on cellular morphology and biochemical criteria the two isolates were tentatively assigned to the genus corynebacterium, although they did not correspond to any recognized species. ...	2009	19567562
identification and characterization of antimicrobial peptide, defensin, in the taiga tick, ixodes persulcatus.	ixodes persulcatus is the primary vector for human tick-borne diseases in japan. a cdna library was constructed from whole body homogenates of fed nymphs of i. persulcatus. from this library, one cdna encoding defensin-like antimicrobial peptide was identified. the amino-acid sequence showed high similarity to those of the defensins of other ticks and arthropods. i. persulcatus defensin mrna transcripts were detected at all life cycle stages of fed ticks and found to be predominantly expressed i ...	2009	19604312
physiological and biochemical consequences of host-plasmid interaction--a case study with corynebacterium renale, a multiple cryptic plasmid containing strain.	corynebacterium renale harbors four small cryptic plasmids, pcr1, pcr2, pcr3 and pcr4, and can be a good system for understanding host-plasmid interactions. in the present study, effect of plasmid loss and their subsequent introduction on various properties of the host was evaluated. loss of plasmids caused a reduction in bacterial size and also slowed down their growth rate, μ, and respiratory rate, r. both μ and r values were partially recovered in c. renale r, obtained by retransformation of ...	2010	21087624
tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick, ixodes persulcatus.	defensins are small cysteine-rich cationic proteins found in both vertebrates and invertebrates constituting the front line of host innate immunity. to examine the importance of the tertiary structure of tick defensin in its antimicrobial activity, we synthesized two types of the peptides with tertiary structure or primary one on basis of the information of the sequence in the defensin originated from the taiga tick, ixodes persulcatus. chemically synthesized peptides were used to investigate th ...	2011	20596886
characterization of corynebacterium species in macaques.	bacteria of the genus corynebacterium are important primary and opportunistic pathogens. many are zoonotic agents. in this report, phenotypic (api coryne analysis), genetic (rpob and 16s rrna gene sequencing), and physical methods (ms) were used to distinguish the closely related diphtheroid species corynebacterium ulcerans and corynebacterium pseudotuberculosis, and to definitively diagnose corynebacterium renale from cephalic implants of rhesus (macaca mulatta) and cynomolgus (macaca fascicula ...	2012	22723254
two rep genes in small cryptic plasmid pkst21 of escherichia coli.	the complete nucleotide sequence of a small cryptic plasmid pkst21 from escherichia coli was determined. this plasmid is 1,460 bp long with an overall gc content of 51 %. based on sequence analysis, the presence of two segments with different average gc density was observed. the segment with higher gc content revealed 98-90 % similarity to several small plasmids of e. coli and to pcr1 from gram-positive corynebacterium renale. plasmid pkst21 possesses two conversely oriented open reading frames ...	2013	23680975
draft genome sequences of two pathogenic corynebacterial species isolated from cows.	the species corynebacterium renale, corynebacterium pilosum, and corynebacterium cystitidis were initially thought to be the same species c. renale, but with different immunological types. these bacteria are the causative agent of cystitis, urethritis and pyelonephritis and are found usually as constituents of the normal flora in the lower urogenital tract of cattle. therefore, we present the draft genome sequences of two pathogenic corynebacterium species: c. renale cip 52.96 and c. pilosum cip ...	2016	26958092
characterization of cimex lectularius (bedbug) defensin peptide and its antimicrobial activity against human skin microflora.	antimicrobial peptides are components of both vertebrate and invertebrate innate immune systems that are expressed in response to exposure to bacterial antigens. naturally occurring antimicrobial peptides from evolutionarily ancient species have been extensively studied and are being developed as potential therapeutics against antibiotic resistant microorganisms. in this study, a putative cimex lectularius (bedbug, cl) defensin is characterized for its effectiveness against human skin flora incl ...	2016	26802465
diphtheroids-important nosocomial pathogens.	diphtheroids are defined as aerobic, non-sporulating, pleomorphic gram-positive bacilli which are more uniformly stained than corynebacterium diphtheriae, lack the metachromatic granules and are arranged in a palisade manner. they are usually commensals of the skin and mucous membranes. they differ from c.diphtheriae in biochemical rea-ctions as well as in toxin production. since, they are usually found as commensals on the skin, they are often considered as mere contaminants when isolated from ...	2016	28208859