Publications

lactobacilli and azoreductase activity in the murine cecum.	azoreductase activity in the ceca of mice lacking lactobacilli as members of the normal microflora (reconstituted-lactobacillus-free [rlf] mice) was compared with that of rlf mice whose gastrointestinal tracts were colonized by strains of lactobacillus delbrueckii and lactobacillus fermentum. azoreductase activity was 31% lower in the ceca of mice colonized by lactobacilli.	1991	1785939
biological utilisation of pearl millet flour fermented with yeasts and lactobacilli.	mixed culture fermentation of pearl millet flour with saccharomyces diastaticus, saccharomyces cerevisiae, lactobacillus brevis and lactobacillus fermentum brought about an improvement in its biological utilisation in rats. protein efficiency ratio, feed efficiency ratio, apparent protein digestibility, true protein digestibility, net protein utilisation, net protein retention, protein retention efficiency and utilisable protein values in case of pure culture fermented pearl millet four were hig ...	1991	1796088
characteristics of lactobacilli isolated from the chicken intestine.	nine strains of lactobacilli isolated from different parts of the intestinal tract of chicken were studied to determine their taxonomy and cultural characteristics. the experimental scheme included physiological and biochemical tests, morphological observations and determination of fermentation products from glucose. all isolates were heterofermentative organisms assigned to the lactobacillus fermentum species. the relevance of intestinal lactobacilli to the nutrition of chickens was discussed.	1991	1906216
antibacterial activity of lactobacillus sake isolated from dry fermented sausages.	lactic acid bacteria isolated from spanish dry fermented sausages were screened for antagonistic activities under conditions that eliminated the effects of low ph and hydrogen peroxide. from 720 isolates tested 119 were inhibitory to lactobacillus fermentum cect285. the isolates showing the largest inhibitory activity exhibited an antagonistic effect against several other lactobacilli and the selected foodborne pathogens staphylococcus aureus and listeria monocytogenes. comparison of the antimic ...	1991	1907472
the stability of thiamine and thiamine tetrahydrofurfuryl disulfide added to table wines.	both thiamine hydrochloride and thiamine tetrahydrofurfuryl disulfide were added separately to table wines at concentrations equivalent to 0.3 and 1.5 microgram of free thiamine per kj of caloric energy. the resultant mean increments in thiamine activity, measured by lactobacillus fermenti microbiological assay after 21 months of storage, were in the range 55 to 103% of the added vitamin, indicative of high bioavailability of thiamine from this source.	1991	1919806
single-stranded dna plasmid, vector construction and cloning of bacillus stearothermophilus alpha-amylase in lactobacillus.	vector plasmids were constructed by ligating chloramphenicol and erythromycin resistance genes to taqi-digested dna of a cryptic plasmid from lactobacillus plantarum. the minimal region of lactobacillus plasmid dna that was required for dna replication was defined and a single-stranded dna intermediate replication system was observed. homologies with other origins of replication of plasmids from gram-positive bacteria, replicating via rolling circle mechanism, were found. it was shown that the c ...	1991	1961976
binding of lactobacillus reuteri to fibronectin immobilized on glass beads.	human fibronectin was immobilized on glass beads. the beads were used to evaluate binding of lactobacillus reuteri to fibronectin. organisms bound to the glass beads were detected using fluorescence microscopy after treatment with acridine orange. this binding was confirmed and quantified with the use of [3h]-labelled organisms. three strains of lactobacillus reuteri, three strains of lactobacillus acidophilus and one strain of lactobacillus fermentum were tested for binding capacity. l. reuteri ...	1992	1303695
adhesion to porcine squamous epithelium of saccharide and protein moieties of lactobacillus fermentum strain 104-s.	the mechanism by which lactobacillus fermentum strain 104-s adheres to porcine squamous epithelium was investigated by studying the adsorption to epithelial cells, and control surfaces, of radioactively labelled material released from the bacterial cells by water extraction. the released material was fractionated by gel filtration and the adsorption of pronase-sensitive and -resistant material in the various fractions to porcine gastric tissue and the control surfaces of polystyrene and immobili ...	1992	1487731
a note on the use of a plasmid as a dna probe in the detection of a lactobacillus fermentum strain in porcine stomach contents.	a plasmid (about 50 kb) was used as a dna probe to enumerate, by colony hybridization, a strain of lactobacillus fermentum in the stomach contents of eight piglets. the population sizes obtained by colony hybridization were in agreement with estimated levels calculated on the basis of plasmid profiling of colonies isolated at random from the total lactobacillus population.	1992	1512179
existence of phosphoenolpyruvate: carbohydrate phosphotransferase systems in lactobacillus fermentum, an obligate heterofermenter.	the presence or absence of the phosphoenolpyruvate: carbohydrate phosphotransferase system (pts) in obligately heterofermentative group iii lactobacilli including lactobacillus brevis (3 strains), l. buchneri (2 strains) and l. fermentum (3 strains) was surveyed systematically for a series of sugars utilizable by these organisms. contrary to common expectation, ptss were found in two strains of l. fermentum: sucrose-pts in one strain; sucrose- and mannose-ptss in the other. all these activities ...	1992	1513267
temperature adaptation in lactobacillus fermentum: interconversions of oleic, vaccenic and dihydrosterulic acids.	the interchange of octadecenoic acids and dihydrosterulic acid was a response of aerobically growing lactobacillus fermentum to changes in growth temperature. oleic and vaccenic acid contents decreased both at temperatures below 20 degrees c and above 26 degrees c, showing mirror image behaviour, with a concomitant increase in dihydrosterulic acid. a temperature-dependent shift from vaccenic to oleic acid synthesis, and the conversion of the latter to dihydrosterulic acid was responsible for the ...	1992	1593259
growth phase, cellular hydrophobicity, and adhesion in vitro of lactobacilli colonizing the keratinizing gastric epithelium in the mouse.	lactobacillus strains of numerous species isolated from several animal sources exhibited cellular hydrophobicities that differed from those expected on the basis of their abilities to colonize the keratinizing stratified squamous epithelium in the mouse stomach. cells of lactobacillus fermentum 100-33, grown to either exponential or stationary phase, were strongly hydrophilic. by contrast, cells of l. fermentum ri and six transformant derivatives of strain ri and 100-33, strains dm101 through dm ...	1992	1622276
2,4,5-trichlorophenoxyacetic acid influence on 2,6-dinitrotoluene-induced urine genotoxicity in fischer 344 rats: effect on gastrointestinal microflora and enzyme activity.	2,4,5-trichlorophenoxyacetic acid (2,4,5-t) and 2,6-dinitrotoluene (2,6-dnt) are hazardous chemicals that have potential harmful effects. 2,6-dnt is recognized as a hepatotoxicant while 2,4,5-t, a component of agent orange, is also suspect. 2,6-dnt requires both oxidative and reductive metabolism to elicit genotoxic effects. to determine what effect 2,4,5-t had on 2,6-dnt metabolism, intestinal enzymes, microbial populations, and urine mutagenicity were examined during 2,4,5-t treatment. weanlin ...	1992	1601224
microbiological methods of thiamine measurement in biological material.	semiautomated, microbiological methods for the determination of thiamine in a variety of biological material are presented. thiamine content was measured in bovine, ovine and rat blood, plasma, bile, urine, duodenal fluid, liver, kidney and brain. thiamine was extracted from samples by mild acid digestion (trans aconitate ph 4.0) at 115 degrees c for 20 min. microbiologic assays utilizing lactobacillus fermenti and ochromonas danica were performed on 96-well microplates and growth, as monitored ...	1992	1587706
lactobacillus cells in the rabbit digestive tract and the factors affecting their distribution.	lactobacillus fermentum was present in small numbers in the caecum and colon of only one of 16 rabbits. studies aimed at elucidating the factors affecting the colonization of lactobacilli in rabbit gut were thus performed. these studies included investigation of the effect of gastric juice and bile salts on the viability of lactobacilli, assay of the survival rates of lactobacilli in the gastrointestinal tracts of the rabbits with or without ileum cannulation and measurement of the adhesive capa ...	1993	8244905
the binding of pneumococcal lipoteichoic acid to human erythrocytes.	previous studies have evaluated the binding characteristics of lipoteichoic acids (lta) composed of polyglycerol phosphate to mammalian cell membranes. this present study was performed to evaluate the binding characteristics of pneumococcal lta, a polyribitol phosphate containing lta, to human erythrocytes. binding was found to be specific, reversible, and dependent on temperature and time. maximum binding was achieved within 2 h at 37 degrees c. scatchard plot analysis of the binding revealed a ...	1993	8391621
expression of glr (muri, dga) gene encoding glutamate racemase in escherichia coli.	the muri (dga) gene of escherichia coli is required for the biosynthesis of d-glutamate, an essential component of bacterial peptidoglycan (doublet, p., van heijetnoort, j., and mengin-lecreulx, d. (1992) j. bacteriol. 174, 5772-5779; dougherty, t. j., thanassi, j. a., and pucci, m. j. (1993) j. bacteriol. 175, 111-116), but its gene product has not been identified. we found that the amino acid sequence of protein deduced from the nucleotide sequence of the open reading frame of muri gene (orf1) ...	1993	7901215
the aggregation of human platelets by lactobacillus species.	the ability to aggregate human platelets was examined for five lactobacillus rhamnosus strains and five lactobacillus paracasei subsp. paracasei strains isolated from patients with infective endocarditis (ie), 25 laboratory isolates from the same two species, and 14 strains from five other oral species, namely lactobacillus acidophilus, lactobacillus fermentum, lactobacillus oris, lactobacillus plantarum and lactobacillus salivarius. amongst the l. rhamnosus strains, platelets were aggregated by ...	1993	8126421
coaggregation of oral lactobacilli with streptococci from the oral cavity.	the ability of oral lactobacilli to coaggregate with streptococci and actinomycetes was investigated. of the 7 species of lactobacilli studied, only two were capable of coaggregation and the coaggregation was restricted to streptococci. lactobacillus salivarius strains (2/4) coaggregated with streptococcus salivarius, streptococcus gordonii, streptococcus crista and tufted streptococcus sanguis ii strains. lactobacillus fermentum (2/3) coaggregated with s. gordonii and s. sanguis. the coaggregat ...	1993	8265207
[studies on the locality and the function of spirosome in bacterial cells: relationship between the production of spirosome and anaerobic glycolysis].	the locality and the function of spirosome were studied by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and electron microscopy in peptostreptococcus productus, clostridium sp. hd-17, lactobacillus fermentum, eubacterium aerofaciens and escherichia cali b. when the bacterial cells in phosphate buffered saline were disrupted by sonication and fractionated by differential centrifugation, the spirosome protein was found in the cytoplasmic fraction and not in the fraction of ...	1993	8315808
purification, cloning, and cofactor independence of glutamate racemase from lactobacillus.	glutamate racemase has been purified more than 12,000-fold from lactobacillus fermenti. the racemase gene has been cloned using standard hybridization techniques combined with a novel selection for in vivo glutamate racemase activity, and the racemase has been expressed in escherichia coli as 20-25% of the total soluble cell protein. the cloned gene product is indistinguishable from that purified from lactobacillus and is a monomer of m(r) 28,300. both a coupled enzymatic assay and a circular di ...	1993	8385993
inhibition of adhesion of escherichia coli k88 to piglet ileal mucus by lactobacillus spp.	enteropathogenic escherichia coli k88 colonizing the piglet ileum adhere to the mucosa by k88 fimbrial appendages. a recent study in our laboratory has implicated indigenous lactobacilli in the suppression of the colonization potential of enteropathogenic e. coli as measured by adhesion to ileal mucus. the aim of this study was to investigate the effect of lactobacillus spp. of porcine origin on the adhesion of k88 fimbriae of e. coli. with an in vitro assay, the adhesion of e. coli k88ab strain ...	1993	8439162
identification of lactobacilli from sourdough and description of lactobacillus pontis sp. nov.	the microflora of sourdough preparations was investigated by examining the physiological characteristics, whole-cell protein patterns, and 16s rrna sequences of lactobacillus isolates. strains isolated from sourdough were placed in the species lactobacillus brevis, lactobacillus sanfrancisco, and lactobacillus reuteri. 16s rrna sequences were determined for l. brevis, lactobacillus fructivorans, lactobacillus fermentum, l. sanfrancisco, and l. reuteri, and oligonucleotide probes for fast specifi ...	1994	8186088
automated microbiological assay of thiamin in serum and red cells.	to develop a sensitive, direct, automated method for the measurement of serum and red cell thiamin.	1994	8089221
genetic modification of a vaginal strain of lactobacillus fermentum and its maintenance within the reproductive tract after intravaginal administration.	many micro-organisms cause important diseases of the female genital tract. because systematic vaccination does not usually provide a good immune response at mucosal sites, commensal lactobacilli from the female genital tract were developed as vehicles to deliver continued doses of foreign antigen directly to the genital mucosal surface with the aim of stimulating strong local mucosal immune responses. lactobacilli were shown to be common inhabitants of the genital tract of the animal model studi ...	1994	7932620
the effect of lactobacillus spp. on the attachment of enterotoxigenic escherichia coli to isolated porcine enterocytes.	a total of 43 strains of lactobacilli were isolated from the gastrointestinal tract of piglets at the time of weaning. isolates, grown on solid media, were allocated to strongly adherent or non/weakly adherent groups on the basis of numbers attaching to isolated porcine enterocytes. strains of lactobacillus fermentum were disproportionally represented amongst the strongly-adherent strains and lact. acidophilus and lact. salivarius amongst the non/weakly-adherent group. lactobacilli showed signif ...	1994	7961193
effect of extracellular lactate on growth of rumen lactate producers.	the addition of na-lactate (50-150 mmol/l) to media with glucose had only marginal effect on the growth of rumen lactate-producing bacteria at ph between 6.5 and 5.8. butyrivibrio fibrisolvens was somewhat more sensitive to external lactate than streptococcus bovis, lactobacillus fermentum and selenomonas ruminantium. it can be concluded that rumen lactate producers, which proliferate at the onset of rumen lactic acidosis, are not influenced by the lactate accumulation, except some non-specific ...	1994	7619002
thiamin status in pregnancy as determined by direct microbiological assay.	serum and red cell thiamin levels were assayed on a group of pregnant australian women using an automated direct microbiological assay with lactobacillus fermenti as the test organism. two of 103 (1.9%) had serum thiamin levels below the reference range and 2 of 67 (3.0%) were found to have red cell thiamin levels below the reference range. although the mean serum levels between trimesters were not significantly different, there was a significant decrease in the mean red cell thiamin in the thir ...	1994	8200746
pathogenic potential of lactobacilli.	lactobacilli are often considered to be commensal or beneficial participants in human microbial ecology and considerable research is being carried out into the effects of the use of lactobacilli as additives in both human and animal diets. however, lactobacilli also cause some human diseases (e.g. dental caries, rheumatic vascular disease, septicaemia and infective endocarditis (ie)), and have recently been identified as potential emerging pathogens in elderly and immunocompromised patients, par ...	1994	7703012
adhesion of lactobacilli to urinary catheters and diapers: effect of surface properties.	thirteen strains of lactobacilli were tested for their ability to adhere to commercial devices used in the urinary tract. although it appeared that the most hydrophilic organisms adhered in highest numbers, there was no significant correlation between water contact angle and adhesiveness to catheters. five organisms tested were found to be highly adherent to huggies commercial diapers. loss in hydrophobicity upon serial culture of lactobacillus fermentum b-54 was not due to a proteinaceous s lay ...	1994	8071384
evaluation of bacterial contamination in a fed-batch alcoholic fermentation process.	alcoholic fermentation by a commercial baker's yeast in a fed-batch process with cell recycling and high-test molasses as substrate was strongly inhibited by lactobacillus fermentum cct 1407 after a few recycles. when total acidity (mainly lactic acid) exceeded 4.8 g/l broth it seriously interfered with yeast bud formation and viability and above 6.0 g/l it decreased alcoholic efficiency.	1994	24421199
chemical modifications of the cell-surface components of lactobacillus fermentum ftpt 1405 and their effect on the flocculation of saccharomyces cerevisiae.	the effect of treatment of lactobacillus fermentum with several protein- and carbohydrate-modifying reagents on the bacterium's ability to flocculate saccharomyces cerevisiae was investigated. the proteinaceous nature of the cell-surface components of l. fermentum which are responsible for floc formation was confirmed by inactivation of floc formation following photo-irradiation, with methylene blue or rose bengal as sensitizer, or acylation with acetic anhydride, maleic anhydride or acetylimida ...	1995	24414903
[a microflora study of the gastrointestinal tract of mink housed within and outside of the area of the chernobyl atomic electric power station].	quantitative differences in the content of lactic acid bacteria isolated from the content of the stomach, small and large intestine have been established when studying microflora of the gastrointestinal tract of minks kept in the 30-kilometer zone of the chernobyl npp (experimental animals) and at the cherkassy fur farm (control animals). obligate heterofermentative species of lactic acid bacteria related to lactobacillus fermentum and l. reuteri prevailed in the stomach of experimental minks. s ...	1995	7663745
cellular fatty acid profiles of lactobacillus and lactococcus strains in relation to the oleic acid content of the cultivation medium.	cellular fatty acids of 10 strains of lactic acid bacteria were analyzed. the purpose of this work was to find lactic acid bacteria with high lactobacillic acid contents. the bacteria studied were unable to synthesize oleic acid. some strains did not synthesize lactobacillic acid, although all were able to form dihydrosterculic acid. twenty-one to thirty-four percent of the fatty acid content of lactobacillus fermentum and l. buchneri was lactobacillic acid, and these species were chosen for fut ...	1995	16535200
lactobacillus fermentum endocarditis involving a native mitral valve.		1995	7650937
genetic analysis of acidocin b, a novel bacteriocin produced by lactobacillus acidophilus.	the genes encoding the production of acidocin b, a bacteriocin produced by lactobacillus acidophilus strain m46 which is active against listeria monocytogenes, clostridium sporogenes, brochothrix thermosphacta, lactobacillus fermentum and lactobacillus delbrueckii subsp. bulgaricus, but inactive against most other lactobacillus species, were previously localized on a 4 kb xbai-hindiii fragment of plasmid pcv461. in the present work, dna sequence analysis revealed the presence of three consecutiv ...	1995	7551031
the antimicrobial activity of lactic acid bacteria from fermented maize (kenkey) and their interactions during fermentation.	a total of 241 lactic acid bacteria belonging to lactobacillus plantarum, pediococcus pentosaceus, lactobacillus fermentum/reuteri and lactobacillus brevis from various processing stages of maize dough fermentation were investigated. results indicated that each processing stage has its own microenvironment with strong antimicrobial activity. about half of the lact. plantarum and practically all of the lact. fermentum/reuteri investigated were shown to inhibit other gram-positive and gram-negativ ...	1995	8567490
adhesion of lactobacillus fermentum 104-s to porcine stomach mucus.	the adhesion to whole and fractionated porcine gastric mucus of both lactobacillus fermentum 104-s cells and a saccharide extracted from this strain was investigated. it has been shown previously that this saccharide had affinity for nonsecreting gastric epithelium. the mucus component(s) with affinity the bacterial cells was partly characterized by gel filtration and treatment with protease or metaperiodate. l. fermentum 104-s extracts containing the saccharide were radioactively labeled, fract ...	1996	8661685
the potential of lactobacillus as a carrier for oral immunization: development and preliminary characterization of vector systems for targeted delivery of antigens.	oral administration of lactobacilli evokes mucosal and systemic immune responses against epitopes associated with these organisms (gerritse et al., 1990, 1991). the adjuvant function of different lactobacillus species was investigated under the conditions of intraperitoneal (i.p.) injection or oral administration. after i.p. injection of trinitrophenylated chicken gamma-globulin, high dth responses were observed with lactobacillus casei and lactobacillus plantarum, but low responses with lactoba ...	1996	8717402
effect of adherent lactobacillus spp. on in vitro adherence of salmonellae to the intestinal epithelial cells of chicken.	single strains of lactobacillus acidophilus and lact. fermentum, isolated from chicken intestine, were used to study in vitro interactions with salmonella enteritidis, salm. pullorum or salm. typhimurium in an ileal epithelial cell (iec) radioactive assay. exclusion, competition and displacement phenomena were investigated by respectively incubating (a) lactobacilli and iec together, prior to addition of salmonellae, (b) lactobacilli, iec and salmonellae together, and (c) salmonellae and iec, fo ...	1996	8760330
inhibition of initial adhesion of uropathogenic enterococcus faecalis by biosurfactants from lactobacillus isolates.	in this study, 15 lactobacillus isolates were found to produce biosurfactants in the mid-exponential and stationary growth phases. the stationary-phase biosurfactants from lactobacillus casei subsp. rhamnosus 36 and atcc 7469, lactobacillus fermentum b54, and lactobacillus acidophilus rc14 were investigated further to determine their capacity to inhibit the initial adhesion of uropathogenic enterococcus faecalis 1131 to glass in a parallel-plate flow chamber. the initial deposition rate of e. fa ...	1996	8787394
effect of lactobacilli and antibiotics on e. coli urinary infections in mice.	urinary antibiotic treatments usually affect the normal urethral flora. this work was developed in order to evaluate whether lactobacillus fermentum crl 1058 could reduce urinary tract infections (uti) produced by uropathogenic escherichia coli in mice treated with antimicrobial agents. animals were inoculated intra-urethrically with agarose beads containing lactobacilli, and were challenged with e. coli. ampicillin (13 mg/kg/dose) was administered orally. the number of microorganisms present at ...	1996	8820918
purification and characterization of a component produced by lactobacillus fermentum that inhibits the adhesion of k88 expressing escherichia coli to porcine ileal mucus.	it has previously been shown that lactobacillus fermentum strain 104r releases compounds into its culture fluid that inhibit the adhesion of enterotoxigenic escherichia coli k88. the aim of the present study was to purify and identify this compound. judged by gel filtration, the compound was found to be approximately 1700 kda. the amount of active compound increased upon prolonged incubation, while the number of viable cells reduced, suggesting that the activity was coming from dead cells. as th ...	1996	8852678
influence of carbohydrates on the alpha-galactosidase activity of lactobacillus fermentum	the influence of soybean galactosaccharides (stachyose, raffinose, melibiose) as well as galactose and glucose on the synthesis and activity of alpha-galactosidase (alpha-gal) from lactobacillus fermentum crl 251 was studied. stachyose was the most effective inducer, followed by melibiose, raffinose, and galactose; scarce activity was detected with glucose. exogenously supplied glucose inhibited the synthesis of the enzyme in cultures of l. fermentum growing on galactose. this effect was reverse ...	1996	8875910
a bifunctional urease enhances survival of pathogenic yersinia enterocolitica and morganella morganii at low ph.	to infect a susceptible host, the gastrointestinal pathogen yersinia enterocolitica must survive passage through the acid environment of the stomach. in this study, we showed that y. enterocolitica serotype o8 survives buffered acidic conditions as low as ph 1.5 for long periods of time provided urea is available. acid tolerance required an unusual cytoplasmically located urease that was activated 780-fold by low-ph conditions. acid tolerance of helicobacter species has also been attributed to u ...	1996	8932305
colonization by lactobacilli of piglet small intestinal mucus.	the colonization potential of lactobacilli was investigated using small intestinal mucus extracts from 35-d-old pigs. mucus-secreting tissue from the small intestine of piglets was gently rinsed to remove contents and then shaken in buffer to release mucus from the surface. numbers of lactobacilli in different portions of the small intestine of 35-d-old pigs were enumerated. also, mucus isolated from the small intestine of pigs was investigated for its capacity to support the growth of lactobaci ...	1996	8939025
[the antagonistic properties of microaerophilic bacteria isolated from the human and mink digestive tracts].	study of antagonistic properties of microaerophilic bacteria isolated from human and mink gastroenteric tract have helped to establish differences in species composition, quantity and level of antagonistic activity of the studied microorganisms in respect to pathogenic microflora. it is shown that lactic acid bacteria identified as lactobacillus fermentum and l. reuteri prevail among the strains isolated from the stomach and thin intestine of minks kept in the 30-km zone of chernobyl npp. specie ...	1996	9044713
probiotic treatment of small intestinal bacterial overgrowth by lactobacillus fermentum kld.	the principle of using harmless bacteria for conquering pathogens has been used for many years. it has been used prophylactically against travellers' diarrhoea and for protection of recurrent pseudomembranous colitis. the aim of this study was to treat a chronic infectious condition, small intestinal bacterial overgrowth, by oral administration of a certain strain of lactobacillus. 17 patients with long-standing bacterial overgrowth of the small intestine were included. the study was designed as ...	1996	9060066
experiences with the use of a starter culture in the fermentation of maize for 'kenkey' production in ghana.	controlled fermentation of maize was carried out using six strains of lactobacillus fermentum and one strain of yeast, saccharomyces cerevisiae, isolated from traditionally fermented maize dough as starter cultures for inoculum enrichement. the fermentations were monitored by ph, acidity, microbiological analysis and taste panel evaluation of two products, kenkey and koko, prepared from the fermented doughs. the strains of l. fermentum used as starter culture dominated the microflora during ferm ...	1996	24415385
feeding resistant starch affects fecal and cecal microflora and short-chain fatty acids in rats.	the effects of different forms of resistant potato starch (rs) on the major microbial population groups and short-chain fatty acids (scfa) in the cecum and feces of rats were studied over a 5-mo feeding period. thirty 8-wk-old male wistar rats, averaging 210 g initial body weight, were adapted for 7 d to a balanced basal diet containing 60% waxy maize starch devoid of any rs. on d 8, three groups of 10 rats each were fed diets containing the following forms of starch: 1) rapidly digestible waxy ...	1997	9303464
identification and characterization of a basic cell surface-located protein from lactobacillus fermentum br11.	extraction of lactobacillus fermentum br11 cells with 5 m licl yielded a preparation containing a single predominant polypeptide with an apparent molecular mass of 32 kda. a clone encoding an immunoreactive 32-kda polypeptide was isolated from a puc18 library of l. fermentum br11 dna by screening with an antiserum raised against whole cells of l. fermentum br11. sequence determination of the insert in the clone revealed a complete 795-bp open reading frame (orf) that defines a 28,625-da polypept ...	1997	9150229
synthesis of nitric oxide from the two equivalent guanidino nitrogens of l-arginine by lactobacillus fermentum.	ten strains of lactobacillus fermentum that differed in origin converted metmyoglobin to nitrosylmyoglobin [a pentacoordinate nitric oxide (no) complex of fe(ii) myoglobin] in mrs broth at ph 4.3. of the 10 strains, l. fermentum ifo 3956 possessed the strongest capacity to convert metmyoglobin to nitrosylmyoglobin. this strain synthesizes no enzymatically from the two equivalent guanidino nitrogens of l-arginine. to our knowledge, this demonstrates for the first time the production of no synthes ...	1997	9401042
microflora of boza, a traditional fermented turkish beverage.	boza, a turkish traditional beverage made by yeast and lactic acid fermentation of cooked maize, wheat and rice flours was prepared and the microbial characteristics were investigated. during the course of fermentation from 0 to 24 h, populations of lactic acid bacteria and yeast raised from 7.6 x 10(6) and 2.25 x 10(5) after inoculation to 4.6 x 10(8) and 8.1 x 10(6), respectively; ph dropped from 6.1 to 3.5; total titratable acidity by means of lactic acid increased from 0.02 to 0.27 mmol/g; a ...	1997	9105937
cell wall anchoring of the streptococcus pyogenes m6 protein in various lactic acid bacteria.	the m6 protein from streptococcus pyogenes is the best-characterized member of a family of cell envelope-associated proteins. based on the observation that the c-terminal sorting signals of these proteins can drive cell wall anchoring of heterologous unanchored proteins, we have cloned and expressed the emm6 structural gene for the m6 protein in various lactic acid bacteria (lab). the emm6 gene was successfully expressed from lactococcal promoters in several lactococcus lactis strains, an animal ...	1997	9139932
use of sulfite and hydrogen peroxide to control bacterial contamination in ethanol fermentation.	lactic acid bacteria isolated from an industrial-scale ethanol fermentation process were used to evaluate sulfite as a bacterial-contamination control agent in a cell-recycled continuous ethanol fermentation process. the viabilities of bacteria were decreased by sulfite at concentrations of 100 to 400 mg liter-1, while sulfite at the same concentrations did not change the viability of the saccharomyces cerevisiae strain used in this process. sulfite was effective only in the presence of oxygen. ...	1997	8979332
nucleotide sequence of the bacillus licheniformis atcc 10716 dat gene and comparison of the predicted amino acid sequence with those of other bacterial species.	the gene encoding the d-aminotransferase from bacillus licheniformis was cloned and the complete dna sequence was determined. the deduced d-aminotransferase protein sequence, consists of 283 amino acids and shows a high degree of homology with other bacillus d-aminotransferases, branched chain aminotransferase of escherichia coli and the 4-amino-benzoate-4-deoxychorismate lyase of bacillus subtilis and escherichia coli.	1997	9003455
effects of lactobacilli on yeast-catalyzed ethanol fermentations.	normal-gravity (22 to 24 degrees plato) wheat mashes were inoculated with five industrially important strains of lactobacilli at approximately 10(5), approximately 10(6), approximately 10(7), approximately 10(8), and approximately 10(9) cfu/ml in order to study the effects of the lactobacilli on yeast growth and ethanol productivity. lactobacillus plantarum, lactobacillus paracasei, lactobacillus #3, lactobacillus rhamnosus, and lactobacillus fermentum were used. controls with yeast cells but no ...	1997	9361399
characterization of the bacterial flora of sudanese sorghum flour and sorghum sourdough.	the microflora of a sudanese sorghum flour, a spontaneously fermented sourdough and a long-term sourdough produced in a sudanese household by consecutive re-inoculations, was studied. the dominant contaminants of sorghum flour were gram-negative, catalase-positive, rod-shaped bacteria with counts of about 10(5) cfu g-1. the spontaneously fermented sorghum sourdough showed a bacterial succession from gram-negative, catalase-positive contaminants to enterococcus faecalis, lactococcus lactis, lacto ...	1997	9449814
role of bacteria in the pathogenesis of short bowel syndrome-associated d-lactic acidemia.	previously, we have demonstrated that short bowel syndrome (sbs) patients suffer daily from d-lactic acidemia; in these patients rather high amounts of (bacterial) d-lactate emerge in blood and urine with a circadian rhythm. the aim of this study was to establish the microbial basis of d-lactic acidemia in sbs. therefore, faecal flora of (young and adult) sbs-patients was analysed qualitatively and quantitatively, and compared to that of controls. the isolated bacterial species were screened for ...	1997	9160298
isolation and characterization of a plasmid from lactobacillus fermentum conferring erythromycin resistance.	lactobacillus fermentum is a lactic acid bacterial species commonly found in the digestive tracts of pigs and rodents and also present in man. we characterized a 5.7-kb plasmid, plem3, conferring erythromycin resistance, which was isolated from a porcine strain of l. fermentum. plasmid plem3 established efficiently in l. fermentum, conferred high-level erythromycin resistance (mic > 1 mg/ml), and was segregationally stable. a deletion derivative of plem3, called plem5, was constructed and found ...	1997	9200223
digoxigenin-labeled deoxyribonucleic acid probes for the enumeration of bifidobacteria in fecal samples.	the numbers of bifidobacteria in fecal samples were specifically determined by colony hybridization with the mixture of digoxigenin-labeled dna probes that were prepared from whole chromosomal dna of bifidobacterium longum 6001 and bifidobacterium adolescentis 6003. these dna probes strongly hybridized with dna of b. longum, b. adolescentis, bifidobacterium breve, bifidobacterium suis, bifidobacterium infantis, bifidobacterium bifidum, bifidobacterium angulatum, and bifidobacterium animalis. det ...	1997	9241587
identification and phylogenetic analysis of lactobacillus using multiplex rapd-pcr.	multiplex rapd-pcr was used to generate unique and identifying dna profiles for isolates of the genus lactobacillus. the method that was used was based on the combination of two 10-mer oligonucleotides in a single pcr. the generated rapd profiles enabled discrimination of all lactobacillus strains that were used in this study. a dendrogram was generated from the rapd profiles. the results of genetic relatedness obtained from the dendrogram were compared with the results obtained using carbohydra ...	1997	9252586
primary peritonitis due to lactobacillus fermentum.		1998	9570668
lactobacillus species associated with active caries lesions.	lactobacilli that are most frequently associated with active caries lesions in dentine were isolated and identified as part of a research project which aims to determine the role of the predominant species of these organisms in the carious process. samples of carious dentine were collected from 12 patients with open caries lesions (group a) and stimulated saliva samples were collected from 12 patients with a dmft = 0 and confirmed presence of lactobacilli in the oral cavity (group b). after seri ...	1998	9588995
colonization capability of orally administered lactobacillus strains in the gut of gnotobiotic piglets.	in the present study, the effect of lactobacillus casei subsp. casei and lactobacillus fermentum inoculation on jejunum and ileum colonization in gnotobiotic piglets has been observed. the characteristic features of the strains used were strong adherence to pig epithelial gut cells as well as inhibitory activity against enteropathogenic e. coli under in vitro conditions. strains were inoculated to 2, 3, and 4 day old gnotobiotic piglets at a dose of 2 ml (1 x 10(8) germs/ml). on the second day a ...	1998	9646555
lactobacilli counts in crop, ileum and caecum of growing broiler chickens fed on practical diets containing whole or dehulled sweet lupin (lupinus angustifolius) seed meal.	1. four experiments with growing broiler chickens were carried out to study the effects of the inclusion in their diets of lupin (lupinus angustifolius) seed meal of e. coli and lactobacilli counts in crop, ileum and caecca at 3 or 4 weeks of age. 2. diets were formulated to contain the same amounts of metabolisable energy (12.55 mj/kg) and protein (210 g/kg). raw whole (heat-untreated) or dehulled sweet (low in alkaloids) lupin seed meal (400 and 320 g/kg respectively) were used to prepare the ...	1998	9693815
microbial populations associated with the retting of melon pods (colocynthis citrullus l.) during seed recovery.	the traditional process for the retting of melon pulp and microbiological characteristics in the recovery of melon seeds (colocynthis citrullus l.) were investigated. melon pods were sliced open and exposed for seven days. the pulp underwent a natural fermentation that was characterized by the growth of microorganisms to 10(8)-10(10) cfu/g. the ph fluctuated between 4.8 and 5.1 with a lactic acid content of 0.72%. bacillus subtilis, b. polymyxa, lactobacillus fermentum, l. brevis and streptococc ...	1998	9839833
development and application of an in vitro methodology to determine the transit tolerance of potentially probiotic lactobacillus and bifidobacterium species in the upper human gastrointestinal tract.	an in vitro methodology which mimics in vivo human upper gastrointestinal transit was developed. the transit tolerance of potentially probiotic lactobacillus and bifidobacterium species was determined by exposing washed cell suspensions at 37 degrees c to a simulated gastric juice (ph 2.0), containing pepsin (0.3% w/v) and sodium chloride (0.5% w/v), and a simulated small intestinal juice (ph 8.0), containing pancreatin usp (1 g l-1) and sodium chloride (5 g l-1), and monitoring changes in total ...	1998	9674129
purification and characterization of cystathionine gamma-lyase from lactobacillus fermentum dt41.	a homo-tetrameric ca. 140-kda cystathionine gamma-lyase was purified to homogeneity from lactobacillus fermentum dt41 by four chromatographic steps. this was the first enzyme responsible for amino acid catabolism purified from lactobacilli. the activity is pyridoxal-5'-phosphate dependent and the enzyme catalyzes the alpha,gamma-elimination reaction of l-cystathionine producing l-cysteine, ammonia and alpha-ketobutyrate. the cystathionine gamma-lyase produced a free thiol group, a keto acid comp ...	1998	9770274
identification of lactic acid bacteria from chili bo, a malaysian food ingredient.	ninety-two strains of lactic acid bacteria (lab) were isolated from a malaysian food ingredient, chili bo, stored for up to 25 days at 28 degreesc with no benzoic acid (product a) or with 7,000 mg of benzoic acid kg-1 (product b). the strains were divided into eight groups by traditional phenotypic tests. a total of 43 strains were selected for comparison of their sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) whole-cell protein patterns with a sds-page database of lab. iso ...	1999	9925588
lectin-like protein fractions in lactic acid bacteria isolated from chickens.	agglutination of fifty bacterial strains isolated from gastric-intestinal tract of chickens with yeasts treated with glutaraldehyde and glycine was assayed to study possible adhesion mechanisms to epithelia. it was found that lactobacillus fermentum, lactobacillus fermentum subsp. cellobiosus and lactobacillus animalis agglutinated yeasts massively and this agglutination was inhibited in each strain by different representative sugars: agglutination was inhibited in three selected strains with 0. ...	1999	9989654
lactobacilli isolated from chicken intestines: potential use as probiotics.	lactobacillus strains were tested for their in vitro probiotic properties. cell surface hydrophobicity was found to be very high for lactobacillus fermentum subsp. cellobiosus and salmonella gallinarum; high values could indicate a greater ability to adhere to epithelial cells. studies on lactobacillus animalis indicated relative cell surface hydrophobicities smaller than those of l. fermentum subsp. cellobiosus and l. fermentum. l. animalis and enterococcus faecalis were able to coaggregate wit ...	1999	10090244
the bspa locus of lactobacillus fermentum br11 encodes an l-cystine uptake system.	bspa is a basic surface-exposed protein from lactobacillus fermentum br11. sequence comparisons have shown that it is a member of family iii of the solute binding proteins. it is 89% identical to the collagen binding protein, cnb, from lactobacillus reuteri. compared with the database of escherichia coli proteins, bspa is most similar to the l-cystine binding protein fliy. to investigate the function of bspa, mutants depleted for bspa were generated by homologous recombination with a temperature ...	1999	10094698
[grounds for use of lactobacterin from lactobacillus fermentum 90-ts-4 in gynecological practice].		1999	10095966
use of conserved randomly amplified polymorphic dna (rapd) fragments and rapd pattern for characterization of lactobacillus fermentum in ghanaian fermented maize dough.	the present work describes the use of randomly amplified polymorphic dna (rapd) for the characterization of 172 dominant lactobacillus isolates from present and previous studies of ghanaian maize fermentation. heterofermentative lactobacilli dominate the fermentation flora, since approximately 85% of the isolates belong to this group. cluster analysis of the rapd profiles obtained showed the presence of two main clusters. cluster 1 included lactobacillus fermentum, whereas cluster 2 comprised th ...	1999	10388723
starter culture activity in refrigerated fermented soymilk.	the refrigerated shelf life of soymilk fermented with single cultures of lactobacillus fermentum, l. casei, streptococcus salivarius subsp. thermophilus, and bifidobacterium longum was evaluated. during storage at 4 degrees c for 28 days, the stability of the microflora differed markedly among the starter cultures. after 28 days, the average numbers of s. salivarius subsp. thermophilus decreased by two log cycles to 6.0 x 10(7) cfu/ml, whereas those of l. casei increased gradually by more than t ...	1999	10419278
identification of and hydrogen peroxide production by fecal and vaginal lactobacilli isolated from japanese women and newborn infants.	we identified lactobacillus isolates from japanese women and newborn infants by a dna-dna hybridization method. the predominating lactobacilli were lactobacillus crispatus and lactobacillus gasseri in the women's vaginas and the newborns' intestines and l. gasseri and lactobacillus fermentum in the women's intestines. all l. crispatus strains were exclusively strong h2o2 producers.	1999	10449509
the broad host range plasmid plf1311 from lactobacillus fermentum vkm1311.	the complete nucleotide sequence (2389 bp) of the cryptic plasmid plf1311 from lactobacillus fermentum vkm1311 was determined. dna sequence analysis revealed the putative coding regions for a replicative protein (repb), its repressor (repa) and double-stranded (dso) and single-stranded (sso) origins. plf1311 belongs to the pe194 family of rolling circle-replicating plasmids. a derivative of plf1311 that contains the cat gene of plasmid pc194 of staphylococcus aureus and the orit of rp4 was const ...	1999	10483722
collagen (cn-i) binding by gut lactobacilli.	four gut lactobacillus strains displaying the features which make them particularly promising for the preparation of probiotic products were investigated together with 5 fresh isolates and one collection strain of lactobacillus plantarum for their ability to bind type i collagen (cn-i). immobilised cn-i in microtitre plates was bound only by 3 strains of gut lactobacilli from piglets and the collection strain lactobacillus plantarum lhi 10 from prague in range of a570nm readings 0.114-0.221. six ...	1999	10486988
expression of chlamydia psittaci- and human immunodeficiency virus-derived antigens on the cell surface of lactobacillus fermentum br11 as fusions to bspa.	the basic surface protein, bspa, has been used as a fusion partner to direct peptide antigens from the human immunodeficiency virus gp41 protein and the chlamydia psittaci ompa protein to the cell surface of lactobacillus fermentum br11. bspa has potential utility in the construction of live vaccines and diagnostic reagents.	1999	10496936
efficiency of transcription from promoter sequence variants in lactobacillus is both strain and context dependent.	the introduction of consensus -35 (ttgaca) and -10 (tataat) hexamers and a tg motif into the lactobacillus acidophilus atcc 4356 wild-type slpa promoter resulted in significant improvements (4.3-, 4.1-, and 10.7-fold, respectively) in transcriptional activity in lactobacillus fermentum br11. in contrast, the same changes resulted in decreased transcription in lactobacillus rhamnosus gg. the tg motif was shown to be important in the context of weak -35 and -10 hexamers (l. fermentum br11) or a co ...	1999	10515955
human cytokine responses induced by gram-positive cell walls of normal intestinal microbiota.	the normal microbiota plays an important role in the health of the host, but little is known of how the human immune system recognizes and responds to gram-positive indigenous bacteria. we have investigated cytokine responses of peripheral blood mononuclear cells (pbmc) to gram-positive cell walls (cw) derived from four common intestinal indigenous bacteria, eubacterium aerofaciens (eu.a. ), eubacterium limosum (eu.l.), lactobacillus casei (l.c.), and lactobacillus fermentum (l.f.). our results ...	1999	10540188
polyphasic study of the spatial distribution of microorganisms in mexican pozol, a fermented maize dough, demonstrates the need for cultivation-independent methods to investigate traditional fermentations.	the distribution of microorganisms in pozol balls, a fermented maize dough, was investigated by a polyphasic approach in which we used both culture-dependent and culture-independent methods, including microbial enumeration, fermentation product analysis, quantification of microbial taxa with 16s rrna-targeted oligonucleotide probes, determination of microbial fingerprints by denaturing gradient gel electrophoresis (dgge), and 16s ribosomal dna gene sequencing. our results demonstrate that dgge f ...	1999	10584005
some probiotic properties of chicken lactobacilli.	the beneficial effect of lactobacilli has been attributed to their ability to colonize human and animal gastrointestinal tracts. in this work, adhesion assays with three lactobacillus strains and intestinal fragments obtained from chickens were assessed. lactobacillus animalis and l. fermentum were able to adhere to three kinds of epithelial cells (crop, small and large intestines) with predominance to small intestine. among the strains considered, l. fermentum subsp. cellobiosus showed the lowe ...	1999	10696476
expression of bacillar glutamyl endopeptidase genes in bacillus subtilis by a new mobilizable single-replicon vector plf.	the plf1311 natural plasmid from lactobacillus fermentum 1311 was used to construct a single-replicon vector suitable for rapid cloning in a wide range of gram-positive hosts and escherichia coli. the new vector is capable of conjugative mobilization from e. coli to various hosts by conjugal transfer. the final vector (3.4 kb) showed a high segregational and structural stability and a high copy number. glutamyl endopeptidase genes from bacillus licheniformis (gsebl) and b. intermedius (gsebi) we ...	2000	10783297
[a family of shuttle vectors for lactobacilli and other gram-positive bacteria based on the plasmid plf1311 replicon].	a set of broad-host-range single-replicon shuttle vectors for cloning nucleotide sequences in gram-positive bacteria (lactobacilli, enterococci, lactococci, bacilli, etc.) was created. the vectors are based on the cryptic plasmid plf1311 from lactobacillus fermentum vkm 1311 belonging to a family of the sigma-type pe194-like plasmids. the vectors can replicate in gram-positive bacteria and escherichia coli. they are stable in many gram-positive bacteria, have small sizes, and allow the selection ...	2000	10808493
bacterial cell wall-induced arthritis: chemical composition and tissue distribution of four lactobacillus strains.	to study what determines the arthritogenicity of bacterial cell walls, cell wall-induced arthritis in the rat was applied, using four strains of lactobacillus. three of the strains used proved to induce chronic arthritis in the rat; all were lactobacillus casei. the cell wall of lactobacillus fermentum did not induce chronic arthritis. all arthritogenic bacterial cell walls had the same peptidoglycan structure, whereas that of l. fermentum was different. likewise, all arthritogenic cell walls we ...	2000	10816508
lactobacillus mucosae sp. nov., a new species with in vitro mucus-binding activity isolated from pig intestine.	a new lactobacillus species from pig small intestine has been identified. in an attempt to isolate lactobacillus reuteri strains carrying the putative colonization-factor gene (mub, for mucus binding) a mub-derived gene probe was used to screen pig intestinal material. a number of isolates were obtained and primary characterization showed that they were gram-positive, catalase-negative, non-spore-forming, non-motile rods. growth occurred at 45 degrees c but not at 15 degrees c and the dna g+c co ...	2000	10826811
pharmacokinetics of lactobacillus plantarum ncimb 8826, lactobacillus fermentum kld, and lactococcus lactis mg 1363 in the human gastrointestinal tract.	genetically modified lactic acid bacteria may be a way to deliver vaccinal epitopes in the gastrointestinal tract.	2000	10848668
rapid identification of 11 human intestinal lactobacillus species by multiplex pcr assays using group- and species-specific primers derived from the 16s-23s rrna intergenic spacer region and its flanking 23s rrna.	rapid and reliable two-step multiplex polymerase chain reaction (pcr) assays were established to identify human intestinal lactobacilli; a multiplex pcr was used for grouping of lactobacilli with a mixture of group-specific primers followed by four multiplex pcr assays with four sorts of species-specific primer mixtures for identification at the species level. primers used were designed from nucleotide sequences of the 16s-23s rrna intergenic spacer region and its flanking 23s rrna gene of membe ...	2000	10856652
analysis of promoter sequences from lactobacillus and lactococcus and their activity in several lactobacillus species.	promoter-active fragments were isolated from the genome of the probiotic organism lactobacillus rhamnosus strain gg using the promoter-probe vector pnz272. these promoter elements, together with a promoter fragment isolated from the vaginal strain lactobacillus fermentum br11 and two previously defined promoters (lactococcus lactis and lactobacillus acidophilus atcc 4356 slpa), were introduced into three strains of lactobacillus. primer-extension analysis was used to map the transcriptional star ...	2000	10896218
effect of probiotic bacteria on prevalence of yeasts in oropharyngeal biofilms on silicone rubber voice prostheses in vitro.	the proliferation of yeasts in the mixed bacterial and fungal biofilms colonising silicone rubber voice prostheses in laryngectomised patients is the main cause of malfunctioning of the valve mechanism on the oesophageal side of the prostheses. indwelling voice prostheses usually have to be replaced every 3-4 months. the consumption of probiotic bacteria is largely motivated by health claims related to the urogenital and lower digestive tract, but not to the upper digestive tract. the present st ...	2000	10933256
biochemical characterization of lactic acid bacteria isolated from spontaneous fermentation of 'almagro' eggplants.	a total of 149 strains of lactic acid bacteria isolated from the spontaneous fermentation of 'almagro' eggplants were characterized and identified. of the isolates, 148 were determined as belonging to the genus lactobacillus. a coccoid, gram-positive database-negative isolate was obtained in the early stages of fermentation. the lactobacillus strains were divided into six groups based on sugar fermentation patterns and other physiological and morphological characteristics, and were identified as ...	2000	10946834
activity of cecropin p1 and fa-ll-37 against urogenital microflora.	two mammalian antimicrobial peptides, fa-ll-37 and cecropin p1, were tested for activity against six uropathogens and five lactobacillus strains by broth microdilution assay. both peptides inhibited escherichia coli at 25 microm (fa-ll-39), and 1.56 microm (cecropin p1), pseudomonas aeruginosa (12.5 microm, and 25 microm), and klebsiella pneumoniae, (50 microm, and 1.56 microm), but not enterococcus faecalis and staphylococcus epidermidis. fa-ll-37 acted bacteriocidally against e. coli and bacte ...	2000	10955957
[the influence of anaerobic bacteria and mycoplasma isolated from the genital tract on mast cell activation].	nowadays, it is known that mast cells, numerously appearing in all organs and being a source of a wide range of mediators and cytokines, are involved both in physiological and pathological processes. the aim of our study was to examine whether vaginal bacteria, especially those participating in bacterial vaginosis, are able to activate mast cells to mediators secretion. the study was done on rat peritoneal mast cells. the mast cells were incubated in vitro with suspensions of bacteroides capillo ...	2000	11286181
microbial community dynamics during production of the mexican fermented maize dough pozol.	the dynamics of the microbial community responsible for the traditional fermentation of maize in the production of mexican pozol was investigated by using a polyphasic approach combining (i) microbial enumerations with culture media, (ii) denaturing gradient gel electrophoresis (dgge) fingerprinting of total community dna with bacterial and eukaryotic primers and sequencing of partial 16s ribosomal dna (rdna) genes, (iii) quantification of rrnas from dominant microbial taxa by using phylogenetic ...	2000	10966374
purification and characterization of a surface-binding protein from lactobacillus fermentum rc-14 that inhibits adhesion of enterococcus faecalis 1131.	lactobacilli have been shown to be important in the maintenance of the healthy urogenital flora. one strain, lactobacillus fermentum rc-14, releases surface-active components which can inhibit adhesion of uropathogenic bacteria. using a quantitative method for determining inhibition of adhesion, a protein with high anti-adhesive properties against enterococcus faecalis 1131 was purified. the n-terminal sequence of the 29-kda protein was identical to that of a collagen-binding protein from lactob ...	2000	10981710
identification of collagen-binding proteins in lactobacillus spp. with surface-enhanced laser desorption/ionization-time of flight proteinchip technology.	biosurfactants produced by lactobacillus fermentum rc-14, l. rhamnosus gr-1 and 36, and l. casei shirota were found to contain proteins that bind to both collagen types iii and vi, as determined by surface-enhanced laser desorption/ionization (seldi)-time of flight mass spectrometry. both collagen types iii and vi immobilized on seldi preactivated proteinchip arrays detected several different sizes (2 to 48 kda) of collagen-binding proteins. overall, the rc-14-produced biosurfactant contained th ...	2000	11010889
probiotic therapy and functional foods for prevention of urinary tract infections: state of the art and science.	in the past year, interest has heightened in the potential for probiotics to prevent urinary tract infection (uti). mainly, this has been due to concerns about antibiotic resistance and recognition of the scientific efficacy of probiotics. the critical factors in any successful application of probiotics to patient care are the scientific basis for selecting probiotic strains and clinical verification that they are effective against the recurrence of uti. three strains--lactobacillus rhamnosus gr ...	2000	11095902
immune response to orally consumed antigens and probiotic bacteria.	the gut mucosal system must fulfil conflicting roles in suppressing immune responses against orally fed antigens (tolerance) while still retaining the ability to respond to potential enteric pathogens. it must also, to a large degree, not mount an immune response against commensal enteric bacteria and the administration of large numbers of probiotic bacteria formulated as dietary supplements in food products. contrary to this dogma, it has been found that feeding ovalbumin as a marker antigen, i ...	2000	10651930