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isolation and amino acid composition of ribosomal proteins from bacillus stearothermophilus.twelve of the proteins from the 30s ribosome of bacillus stearothermophilus were isolated by preparative disc electrophoresis. amino acid analyses of these proteins showed them to be different from each other. the gross amino acid composition of 30s ribosomal protein from b. stearothermophilus and escherichia coli are virtually identical. a number of the proteins of b. stearothermophilus had electrophoretic mobilities similar or identical to 30s ribosomal proteins of e. coli. however, there was ...19694891258
surface structure of bacillus stearothermophilus ribosomes.no significant differences were noted in either the size or in the arrangement of the surface filaments of the ribosomes of bacillus stearothermophilus and escherichia coli.19694891269
physiological role of 70s ribosomes in bacteria.evidence is presented indicating that free 70s ribosomes are real components of bacillus stearothermophilus and escherichia coli in exponential and stationary phases of growth. after pulses of radioactive leucine or uracil were given, protein synthesis was instantaneously stopped by chloramphenicol, or polypeptide growth was allowed to proceed to completion in slowly cooled cells. analysis of label distribution in sucrose gradients showed that 70s monomers, and not subunits, are final products o ...19694894332
purification and properties of dna-dependent rna-polymerase from bacillus stearothermophilus. 19694894537
dissociation of 70s ribosomes: some properties of the dissociating factor from bacillus stearothermophilus and escherichia coli.a protein factor which produces in vitro dissociation of 70s particles into 30s and 50s subunits has been obtained from bacillus stearothermophilus and escherichia coli. the factor could be extracted from ribosomes, polyribosomes, and s100 supernatant. the kinetics and temperature curve of the dissociation process in the b. stearothermophilus system have been studied and compared with the reaction in the e. coli system. no species specificity was observed when hybrid mixtures of ribosomes and di ...19694901704
purification and comparative properties of a pyrimidine nucleoside phosphorylase from bacillus stearothermophilus. 19694978445
thermophilic aminopeptidases from bacillus stearothermophilus. i. isolation, specificity, and general properties of the thermostable aminopeptidase i. 19695001155
spore formation by bacillus stearothermophilus. 19695358098
[the effect of abrupt temperature shift on the multiplication of bacillus stearothermophilus]. 19695379820
[fine structure of the mesosomes in bacillus stearothermophilus and bacillus coagulans]. 19695398304
phospholipids from bacillus stearothermophilus.the lipids of bacillus stearothermophilus strain 2184 were extracted with chloroform-methanol and separated into neutral lipid and three phospholipid fractions by chromatography on silicic acid columns. the phospholipids were identified by specific staining reactions on silicic acid-impregnated paper, by chromatography of alkaline and acid hydrolysis products, and by determination of acyl ester:glycerol:nitrogen:phosphorus molar ratios. the total extractable lipid was 8% of the dry weight of who ...19695764328
dipicolinate-induced germination of bacillus stearothermophilus spores. 19695764345
the extracellular alpha-amylase of bacillus stearothermophilus. 19695776281
isolation of spheroplast membranes and stability of spheroplasts of bacillus stearothermophilus.spheroplasts were prepared by lysozyme digestion of the cell wall and ruptured by suspension in 0.15 m nacl, followed by centrifugation at 30,900 x g for 35 min, and by a final suspension in 0.05 m nacl for 12 to 16 hr at 5 c. the membrane ghosts were washed four times in tris(hydroxylmethyl)aminomethane (tris) magnesium buffer and once in distilled water. the intact membranes resembled empty sacs with narrow slits in which the cytoplasm was extruded. a 92% recovery of cell membrane was obtained ...19695777801
characteristics of the deoxyribonucleic acid of t phi 3, a bacteriophage for bacillus stearothermophilus.it is shown that the individual strands of bacteriophage tphi3 dna are intact and that heat-denatured tphi3 dna forms a bimodal distribution in a neutral cscl density gradient.19695786184
influence of oxygen on development of nitrate respiration in bacillus stearothermophilus.a denitrifying mutant of bacillus stearothermophilus nca 2184, strain 2184-d, was used to explore the development of nitrate respiration in relation to oxygen respiration. aerobically grown wild-type cultures could acquire the ability to use nitrate as a result of selection of nitrate-respiring mutants by the presence of nitrate and a reduced oxygen tension. fluctuation analysis has revealed that the frequency of occurrence of the nitrate-respiring mutant is about 7.5 x 10(-8) per bacterium per ...19695788697
cell-free amino acid-incorporating systems from bacillus licheniformis and bacillus stearothermophilus 10.cell-free amino acid-incorporating systems were prepared from log-phase cells of bacillus licheniformis and b. stearothermophilus 10. no differences were observed between the two systems with respect to the types and relative amounts of the ribosomes, the time dependence of incorporation, and the optimal magnesium ion concentration (4 mm). the b. licheniformis system showed greater dependence on added transfer ribonucleic acid (trna) and was less stimulated by polyuridylic acid (poly u) than the ...19695788703
the effect of sodium chloride on heat resistance and recovery of heated spores of bacillus stearothermophilus. 19695791939
infection of bacillus stearothermophilus with bacteriophage deoxyribonucleic acid.early stationary-phase cells are most susceptible to infection with deoxyribonucleic acid from bacteriophage tp-1c. transfection is destroyed by deoxyribonuclease and unaffected by phage antiserum.19695802614
the binding of spermine to the ribosomes and ribosomal ribonucleic acid from bacillus stearothermophilus.1. the total intracellular concentrations of na(+), k(+), mg(2+), spermine, spermidine and rna were measured in bacillus stearothermophilus. 2. the binding of spermine to ribosomes and to ribosomal rna from b. stearothermophilus was studied under various conditions by using a gel-filtration technique. 3. the affinity of spermine for ribosomes and for ribosomal rna decreased with increasing ionic strength of the medium in which they were suspended. 4. the extent of spermine binding did not change ...19695806384
prototrophic thermophilic bacillus: isolation, properties, and kinetics of growth.a thermophilic bacillus (minimal growth temperature 41 c, optimal 55 to 58 c, and maximal 65 c) was isolated from a manure pile. it is very similar to bacillus stearothermophilus, but it differs in its inability to hydrolyze starch. the thermophilic isolate is a prototroph which grows in a minimal medium consisting of glucose, ammonium salt, phosphate buffer, and inorganic salts. at all temperatures studied (low to high), the same minimal nutritional requirements prevailed. the arrhenius constan ...19695808072
purification and some properties of leucyl-trna synthetase from bacillus stearothermophilus. 19695823096
[purification and some properties of bacillus stearothermophilus leucyl-trna synthetase]. 19694186386
the amino acid composition and other properties of thermostable glyceraldehyde 3-phosphate dehydrogenase from bacillus stearothermophilus. 19694304231
comparative studies on the isoleucyl- and leucyl-trna synthetases from bacillus stearothermophilus and escherichia coli: thermal stability of the aminoacyladenylate-enzyme complexes. 196911947192
some properties of the leucine-specific trna's from bacillus stearothermophilus. 196911947294
isolation and some properties of the triphosphopyridine nucleotide isocitrate dehydrogenase from bacillus stearothermophilus. 19704393512
properties and regulation of pyruvate carboxylase from bacillus stearothermophilus. 19704394197
studies on thermophilic alpha-amylase from bacillus stearothermophilus. i. some general and physico-chemical properties of thermophilic alpha-amylase. 19705416895
studies on thermophilic alpha-amylase from bacillus stearothermophilus. 3. effect of temperature on the renaturation of denatured thermophilic alpha-amylase. 19705416896
chemical states of bacterial spores: heat resistance and its kinetics at intermediate water activity.bacterial spore heat resistance at intermediate water activity, like aqueous and strictly dry heat resistance, is a property manipulatable by chemical pretreatments of the dormant mature spore. heat resistances differ widely, and survival is prominently nonlogarithmic for both chemical forms of the spore. log survival varies approximately as the cube of time for the resistant state of bacillus stearothermophilus spores and as the square of time for the sensitive state. a method for measuring hea ...19705418938
concerted feedback inhibition of aspartokinase from bacillus stearothermophilus. i. catalytic and regulatory properties. 19705423383
fatty acids from vegetative cells and spores of bacillus stearothermophilus.in spores of bacillus stearothermophilus produced at 45 and 55 c, branched-chain fatty acids predominated in the former and straight-chain acids in the latter.19705429727
[flagellar apparatus in bacillus stearothermophilus and bacillus coagulans]. 19705469673
enzymological differences of alpha-amylase from bacillus stearothermophilus grown at 37 degrees c and 55 degrees c. 19705477223
studies on the subunit structure of thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. 19705497141
fracture faces in intact cells and protoplasts of bacillus stearothermophilus. a study by conventional freeze-etching and freeze-etching of corresponding fracture moieties. 19705507821
a digital computer program for the statistical analysis of heat resistance data applied to bacillus stearothermophilus spores. 19705516592
chemical and morphological changes in bacillus stearothermophilus induced by copper. 19705526726
thermostable aldolase from thermus aquaticus.data are presented on the purification and properties of the thermostable fructose-1,6-diphosphate aldolase of thermus aquaticus, a nonsporulating, extreme thermophile. the enzyme shows little activity at temperatures below 60 c and optimal activity at about 95 c. the enzyme was purified 43-fold by diethylaminoethyl cellulose column chromatography and sephadex g-200 gel filtration. the enzyme is activated by high concentrations of nh(4) (+) and low concentrations of fe(2+) and co(2+) and is stro ...19704984076
studies on thermophilic alpha-amylase from bacillus stearothermophilus. ii. thermal stability of thermophilic alpha-amylase. 19704984659
characterization of the bacillus stearothermophilus phage phimu-4 and its dna. 19704910441
autolysis of cell walls of bacillus stearothermophilus b65 and the chemical structure of the peptidoglycan.1. the cell walls of bacillus stearothermophilus b65 contain glucosamine, muramic acid, alanine, alphain-diaminopimelic acid (dap), glutamic acid, aspartic acid, glycine, and serine in the molecular proportions 0.60:0.64:2.30:0.85:1.00:0.11:0.13:0.31. 2. both d- and l-alanine are present, but glutamic acid and diaminopimelic acid are present only as the d- and meso-isomers respectively. 3. the peptide fragments ala-dap, dap-ala, and dap-ala-dap have been isolated from a partial acid hydrolysate ...19704248963
peptidoglycan synthesis in bacilli. i. effect of temperature on the in vitro system from bacillus megaterium and bacillus stearothermophilus. 19714255188
structure of the cell wall of bacillus stearothermophiluys: mode of action of a thermophilic bacteriophage lytic enzyme.the mode of action of a bacteriophage lytic enzyme on cell walls of bacillus stearothermophilus (nca 1503-4r) has been investigated. the enzyme is an endopeptidase which catalyzes the hydrolysis of the l-alanyl-d-glutamyl linkage in peptide subunits of the cell wall peptidoglycan. preliminary studies on the soluble components in lytic cell wall digests indicate that the glycan moiety is composed of alternating glucosamine and muramic acid; one half of the muramic acid residues contain the tripep ...19714255338
[bacillus stearothermophilus strain growing on an agarized mineral medium with liquid paraffin]. 19714256891
in search of lactate dehydrogenase from bacillus stearothermophilus. 19714257057
production of bacillus stearothermophilus nca 1503 for glyceraldehyde-3-phosphate dehydrogenase (gpdh). 19714933805
mode of action of the competence-inducing factor of bacillus stearothermophilus.development of competence in the noncompetent mutant of bacillus stearothermophilus 4s requires a soluble competence-inducing factor (cf). this study describes several observations that define the mechanism of action of this factor. (i) cf interacts with cells, protoplasts, or cytoplasmic membranes in a temperature-dependent reaction; incubation of cf with cells alters the sedimentation properties of the cells. (ii) association between cf and the cytoplasmic membrane appears to be the first and ...19714934067
factors affecting transfection in bacillus stearothermophilus.the conditions for the infection of bacillus stearothermophilus 4s with tp-1c phage deoxyribonucleic acid (dna) are described. cells from log-phase cultures are the most susceptible to phage dna infection (transfection). a cellular component (competence factor) which enhances transfection is released into the culture medium during the transition period between the log and stationary phase of growth. transfection is stimulated in the order of decreasing effectiveness, by fe(3+), mn(2+), and mg(2+ ...19714934070
temperature dependence of the aminoacylation of trna by bacillus stearothermophilus aminoacyl-trna synthetases. 19714940767
heat stabilities of ribosomal subunits and reassociated ribosomes from bacillus stearothermophilus.absorbance-temperature profiles reveal that both the 30s and 50s ribosomal subunits from bacillus stearothermophilus are more thermostable than the comparable escherichia coli particles. thermophile ribosomes formed by the reassociation of subunits do not display functional heat stability.19714941572
association factor of ribosomal subunits from bacillus stearothermophilus.the isolation of a new factor, which can cause the in vitro association of 30s and 50s ribosomal subunits at low mg(++) concentration, is described. the association factor is eluted together with the dissociation protein when ribosomes of bacillus stearothermophilus are washed with salt solutions of high concentration. the association activity is heat-stable, whereas dissociation factor is inactivated after 10 min at 80 degrees c. this treatment allows the separation of both factors. several pro ...19714941986
bacillus stearothermophilus (donk), a strongly heat-resistant thermophilic contaminant in prepared dehydrated nutrient agar. 19714942975
intracellular proteases of bacillus stearothermophilus.cell-free extracts of bacillus stearothermophilus have been shown to exhibit proteolytic activity toward casein as well as specific activity to catalyze the hydrolysis of furylacryloylglycyl-l-leucine amide, furylacryloylglycine, and carbobenzoxyl-glycine-p-nitrophenyl ester, indicating the presence of a neutral proteinase, a carboxypeptidase-like enzyme, and an alkaline proteinase. the neutral proteinase and carboxypeptidase-like activities were separated by gel filtration over bio-gel p-60, an ...19715002894
turnover and thermostability of proteins and nucleic acids in bacillus stearothermophilus. 19715556683
competence-inducing factor of bacillus stearothermophilus.an incompetent mutant (4s com(-)) does not release competence-inducing factor (cf) into the culture medium and is not infected with tp-1c phage deoxyribonucleic acid (dna) unless cf is added to the transfection assay. a tp-1c phage-resistant mutant (4s ton-r) releases relatively large amounts of cf into the culture medium but is not infected with tp-1c phage dna, even in the presence of cf. the production of cf by the wild type or ton-r mutant and the ability of the wild type or com(-) mutant to ...19715557600
relative incorporation of phenylalanine at different temperatures by cell-free systems from bacillus licheniformis and bacillus stearothermophilus. 19714993725
purification and characterization of glucose-6-phosphate isomerase from bacillus stearothermophilus. 19715117529
biosynthetic l-threonine deaminase from bacillus stearothermophilus. i. catalytic and regulatory properties. 19715123148
synthesis of pyruvate carboxylase from its apoenzyme and (+)-biotin in bacillus stearothermophilus. purification and properties of the apoenzyme and the holoenzyme synthetase.1. methods are described for the assay and purification of pyruvate apocarboxylase and pyruvate holocarboxylase synthetase from biotin-deficient bacillus stearothermophilus. 2. pyruvate apocarboxylase was obtained 200-fold purified and in a nearly homogeneous state; it closely resembled the holoenzyme of the thermophile in fractionation properties, electrophoretic mobility and molecular weight (estimated to be 350000 by gel filtration). 3. pyruvate holocarboxylase synthetase, purified more than ...19715129257
synthesis of pyruvate carboxylase from its apoenzyme and (+)-biotin in bacillus stearothermophilus. mechanism and control of the reaction.1. acetyl-coa acts as a positive allosteric effector in the formation of active pyruvate carboxylase from its apoenzyme, atp and (+)-biotin which is catalysed by holoenzyme synthetase; this effect is counteracted by l-aspartate. 2. the hill coefficients (apparent n values) were approximately 2 for acetyl-coa and 4 for l-aspartate; the n value for each effector remained constant when the concentration of the other effector was varied. 3. active pyruvate carboxylase was formed also when the apoenz ...19715129260
triose phosphate isomerase from bacillus stearothermophilus. 19715131007
rapid quantitative microbiological assay of antibiotics and chemical preservatives of a nonantibiotic nature.a rapid quantitative assay of 14 antibiotics and 6 chemical preservatives by the cylinder-plate diffusion method is described, in which bacillus stearothermophilus atcc 12980 was used as the test organism. after 4 hr of incubation at 65 c, the zones of inhibition can be read off easily with the naked eye. the 4-hr assay was compared under identical conditions with the conventional 16- to 18-hr agar diffusion assay, i.e., by using the test organisms and media proposed by the code of federal regul ...19715132091
the lipid content of bacillus stearothermophilus at 37 degrees and at 55 degrees. 19715146842
catalytic and regulatory properties of meso-diaminopimelate-sensitive aspartokinase from bacillus stearothermophilus. 19715167534
role of 5s rna in the functions of 50s ribosomal subunits.50s ribosomal subunits from bacillus stearothermophilus can be reconstituted from their dissociated components, namely a 5s rna-free protein fraction, a 5s rna-free 23s ribosomal rna fraction, and purified 5s rna. the biological activity of reconstituted particles in polypeptide synthesis is dependent on the presence of 5s rna. in the absence of 5s rna, particles are produced that have greatly reduced activity in (a) polypeptide synthesis directed by synthetic, as well as natural, messenger rna, ...19715289238
homology of ribosomal ribonucleic acid diverse bacterial species with escherichia coli and bacillus stearothermophilus.hybridization competition experiments were used to examine the ribosomal ribonucleic acid (rrna) homologies of 22 bacteria and 3 higher organisms with escherichia coli and bacillus stearothermophilus. although little or no homology was observed with the higher organisms, the bacteria showed a wide range of homologies. organisms whose rrna showed closer homology to e. coli rrna showed less rrna homology to b. stearothermophilus rrna and vice versa.19714329734
glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. 197111945671
tosylphenylalanyl chloromethane-inhibitor of complex of s(1)s(3)-factors in cell-free protein-synthetizing system from bacillus stearothermophilus. 197111946069
comparative studies on the isoleucyl-trna synthetases from bacillus stearothermophilus and escherichia coli: ii. the effect of magnesium ions in the transacylation reaction. 197111946158
neutral proteases with different thermostabilities from a facultative strain of bacillus stearothermophilus grown at 40 degrees and at 50 degrees. 197211946772
amino acid sequence around the catalytic site in glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus.the tryptic peptide containing the active-site cysteine in glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus 1503 was isolated after inhibition of the enzyme with (14)c-iodoacetate. the amino acid sequence of the 20-residue peptide was determined by 19 successive cycles of dansyl-edman degradation. the sequence shows considerable homology with its counterparts from mesophilic sources but differs by the addition of ala-his-his at the n-terminus and by the substitution of p ...197216559157
localization and solubilization of the respiratory nitrate reductase of bacillus stearothermophilus.membranes were isolated from bacillus stearothermophilus 2184d by lysozyme digestion of the cell wall and subsequent differential centrifugation. observations with the electron microscope indicate that such membranes are relatively intact and have a typical membrane appearance. nitrate will preferentially oxidize the cytochrome b of such membranes. approximately 80% of the total respiratory nitrate reductase activity of whole cells can be localized in the washed membrane fraction and the process ...19724333609
physical aggregation and functional reconstitution of solubilized membranes of bacillus stearothermophilus.isolated membranes of bacillus stearothermophilus 2184d can be disrupted by treatment with sodium dodecyl sulfate (sds). this disruption is attended by a decreased turbidity of membrane suspensions and a differential loss of activities of the electron transport system. reduced methyl viologen (mvh)-nitrate reductase activity is insensitive to sds treatment, whereas reduced nicotinamide adenine dinucleotide (nadh)-nitrate reductase and cyanide-sensitive nadh oxidase activities are decreased by 80 ...19724333610
isoleucyl-transfer ribonucleic acid synthetase from bacillus stearothermophilus. i. properties of the enzyme. 19724336851
isoleucyl-transfer ribonucleic acid synthetase from bacillus stearothermophilus. 2. dynamics of the enzyme-substrate interactions. 19724336852
thermostable glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. i. immunochemical studies. 19724338311
arginyl-transfer ribonucleic-acid synthetase from bacillus stearothermophilus. purification, properties and mechanism of action. 19724351436
study of two bacteriophages of bacillus stearothermophilus strain nca1518.studies of bacteriophages gh5 and gh8 of bacillus stearothermophilus strain nca1518 revealed that their properties were sufficiently different from known phages and from each other to indicate that each was a different entity, although no major deviation was demonstrated.19725014935
glucan biosynthesis in bacillus stearothermophilus. i. properties of the polysaccharide. 19725017254
elevated diaminopimelate-sensitive aspartokinase activity during sporulation of bacillus stearothermophilus. 19725021985
[l-leucyl-sarcosyl-l-phenylalanine, synthesis and effect on aminopeptidases i and ii from bacillus stearothermophilus]. 19725023591
the phospholipids of a faculatively thermophilic strain of bacillus stearothermophilus. 19725074272
a unified hypothesis on the causes of the cardinal temperatures of microorganisms; the temperature minimum of bacillus stearothermophilus. 19725074610
the location of spermine in bacterial ribosomes as indicated by 1,5-difluoro-2,4-dinitrobenzene and by ethidium bromide.1. when the binding of ethidium bromide to rrna is measured both in the presence and in the absence of spermine, by spectrophotometric titrations, by gel filtration, or by the changes in fluorescence intensity, spermine competes with ethidium bromide for sites on the rrna; under the conditions used in these experiments ethidium bromide is bound to the double-stranded regions of rrna. 2. when an excess of ethidium bromide is added to ribosomes from bacillus stearothermophilus approx. 80% of the e ...19725084791
substrate inactivation of fructose-i,6-diphosphate aldolase from bacillus stearothermophilus. 19724622933
alumina--atached spores of bacillus stearothermophilus for the control of sterilization process. 19724627038
alumina--attached spores of bacillus stearothermophilus for the control of sterilization process. 19724630308
isolation of the intact strands of the deoxyribonucleic acid of tphi3, a bacteriophage for bacillus stearothermophilus. 19724639277
subunit structure of the thermophilic aminopeptidase i from bacillus stearothermophilus. 19724655250
the effect of spermine on the thermal denaturation profiles of ribosomal rna and of ribosomes from bacillus stearothermophilus in the presence of physiological concentrations of cations. 19724658856
inhibition of peptidoglycan synthesis by the antibiotic diumycin a.diumycin a, a new antibiotic, was found to inhibit cell wall synthesis by staphylococcus aureus, a phenomenon accompanied by accumulation of uridine-5'-diphosphate-n-acetyl-muramyl-pentapeptide. the antibiotic inhibited in vitro peptidoglycan synthesis by particulate preparations of bacillus stearothermophilus and escherichia coli by preventing the utilization of n-acetyl-glucosamine-n-acetyl-muramyl-pentapeptide. in contrast to vancomycin, the antibiotics diumycin, prasinomycin, moenomycin, 11. ...19724670441
preliminary separation of enzymes extracted from bacillus stearothermophilus. 19724263100
specific ribonucleoprotein fragments from the 30-s ribosomal subunits of halobacterium cutirubrum, escherichia coli and bacillus stearothermophilus. 19724609470
studies on variants of bacillus stearothermophilus strain nca 1518.the heat resistance, fermentation reactions, nutritional requirements, and phage sensitivity of 18 selected morphological variants of bacillus stearothermophilus nca 1518 were studied. it was found that when smooth variants mutated to rough colonial morphology, there was no concurrent change in fermentation reactions, nutritional requirements, or heat resistance. the smooth variant, and the rough mutants derived directly from it, presented a uniform pattern of biochemical capabilities which diff ...19724553138
identification of named cultures of bacillus calidolactis as bacillus stearothermophilus.a study was made of 23 cultures of bacillus calidolactis. by using cultural, biochemical, and per cent guanine plus cytosine methods, the cultures were found to be b. stearothermophilus.19724553846
sporulation and heat resistance of bacillus stearothermophilus spores produced in chemically defined media.the effect of amino acids on sporulation is discussed. heat-resistant spores were produced in a chemically defined medium.19724553847
hazard inherent in microbial tracers: reduction of risk by the use of bacillus stearothermophilus spores in aerobiology.the use of a "biological tracer" forms an essential part of many aerobiological experiments. where release of such tracers is likely to result in deliberate or inadvertent human exposure, safety becomes a primary consideration in the selection of the tracer organism. of the three most commonly used organisms, namely bacillus subtilis, escherichia coli, and serratia marcescens, only the first comes near to satisfying the need for nonpathogenicity and even it has been incriminated as a cause of hu ...19724557557
studies on a thermophilic bacillus: its isolation, properties, and temperature coefficient of growth.a thermophilic bacillus with minimal, optimal, and maximal growth temperatures of 40, 64.5, and 72 c, respectively, was isolated from soil. biochemical and morphological studies place the isolate in group 1 of the classification of walker and wolf. after adaption to nitrate broth, the temperature coefficient for growth was found to be 20,400 cal/mol. when the temperature coefficient for growth of the isolate, psychrophilic bacteria, mesophilic bacteria, and a strain of bacillus stearothermophilu ...19724565524
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