Publications

lipid composition of bovine serum lipoproteins.	the four classes of lipoproteins (one of very low density, two of low density, and one of high density) were isolated from the serum of a lactating friesian cow. the proportions of protein and of the different lipid classes were determined in each lipoprotein. triglycerides predominated in the very low density lipoprotein, and cholesteryl esters and phospholipids in the others. the triglycerides of the very low density lipoprotein were richer in oleic acid than were those of the low density lipo ...	1975	163266
mechanism of the suppressive effect of interferon on antibody synthesis in vivo.	mouse interferon preparations significantly suppress the in vivo antibody response to sheep red blood cells (srbc), a thymus-dependent antigen, and to salmonella typhimurium lipopolysaccharide (lps), a thymus-independent antigen. it is also possible to effect the late responses of antigen sensitive "memory" cells observed during secondary immunization by administration of interferon prior to primary immunization. the immunosuppressive activity of interferon was time- and dose-dependent. maximum ...	1975	163863
correlation of estrogen-induced uterine eosinophilia with other parameters of estrogen stimulation, produced with estradiol-17beta and estriol.	estradiol-17beta is a stronger estrogen than estriol for the genomic response of estrogens. estriol is a stronger estrogen than estradiol-17beta for the estrogen-induced uterine eosinophilia and the 6 h increase in the uterine wet weight.	1975	169148
[in vitro evaluation of antiviral inhibitors by means of a turbidity method (author's transl)].	the paper presents a photometric method for the determination of virus-induced alterations (cytopathogenic effects) of cells based on nephelometric analysis. these were taken at varying time intervals after infection and with different multiplicity of infection. it was demonstrated that suppression of virus-induced cpe by the known inhibitors could also be followed by turbidity measurements. the in vitro experiments were carried out in the 2 following virus-cell-systems: pseudorabies virus in ch ...	1975	171906
studies of genetic transmission of mammary tumour virus by c3hf mice.	by radioimmunoassay (ria) mammary tumour virus (mtv) antigens were detected in individual milk samples of c3hf mice, (female balb/c x male c3hf)f1 mice and (female c3hf x male balb/c)f1 mice; milk samples of balb/c mice were negative. in the segregating backcross i population, female balb/c x male (female balb/c x male c3hf) viral antigens were found in the milk of 93 out of 169 mice (55%). in the bc ii population (daughters of bc i mothers and balb/c fathers) two groups were distinguished. in t ...	1975	172460
mapping and gene conversion studies with the structural gene for iso-1-cytochrome c in yeast.	we have investigated the order of the four genes cyc1, rad7, sup4, and cdc8 which form a tightly linked cluster on the right arm of chromosome x in the yeast saccharomyces cerevisiae. crossing over and coconversion data from tetrad analysis established the gene order to be centromere-cyc1-rad7-sup4. also cdc8 appeared to be distal to sup4 on the basis of crossovers that were associated with conversion of sup4. the frequencies of recombination and the occurrence of coconversions suggest that thes ...	1975	174970
steroid production by quartered and capsular rat adrenal gland in response to haemorrhage.	60 min after rapid bleeding (1.5--2.0 per cent of b. w.) both aldosterone and corticosterone production rate by quartered rat adrenals were found to be elevated. however, no difference was observed in the rate of aldosterone and corticosterone production by capsular adrenals of sham operated and hypovolaemic rats. corticosterone production rate by decapsulated adrenals was much more higher after haemorrhage than in the control group. the same alterations could be observed incubating adrenal tiss ...	1975	179800
intrasellar abscess.	a case of intrasellar abscess in a 31-year-old male, with pituitary insufficiency, visual field and acuity disturbances, is reported. in the pus extracted at surgery e. coli and staphylococcus haemolyticus were identified. the patient died 20 days after the operation. necropsy revealed the fibrous capsule of an abscess in the hypophyseal region and putriform tissue at the level of the hypothalamus, with three small tumours one of which proved to be a craniopharyngioma.	1975	765877
[the acute vestibular paralysis (author's transl)].	acute vestibular paralysis may not be considered as a nosologic entity but as a syndrome. symptomatology (vertigo, spontaneous and provoked vestibular nystagmus, absence of cochlear signs) shows an uniform picture. the results of the caloric test as well as the nystagmic responses induced by galvanic stimulation and the development of central vestibular compensation however indicate that the site of the lesion is not only confined to the labyrinth but may also occur at the level of the periphera ...	1975	1081389
morphology of the perivascular regions of the rat neural lobe in relation to hormone release.	neural lobes of rats subjected to dehydration by drinking 2% saline for four days were examined electron microscopically and compared to untreated controls. the ultrastructure of the blood vessels and the tissues surrounding them were examined and it was found that, although few exocytotic figures could be seen in either group of animals, a significantly larger (p smaller than 0.01) number os small vesicles were found in nerve endings adjacent to the perivascular space in the saline treated grou ...	1975	1149110
[the effect of serotonin on the release of free fatty acids from human and rat adipose tissue (author's transl)].		1975	1156892
virus infections and kidney disease.		1975	1196274
changes in blood flow to bones during the hypocalcemic and hypercalcemic phases of the response to parathyroid hormone.	labeled microspheres were used to measure blood flow to the leg bones of the laying hen at 0, 3, and 30 min after iv injection of parathyroid hormone (pth) (wilson) or the synthetic 1 to 34 fragment of bovine parathyroid hormone (pth 1-34). at 3 min, which corresponds to the hypocalcemic phase of the pth response, blood flow to the combined femur, tibia, and metatarus was significantly reduced by pth (wilson) relative to 0 time and to carrier-injected controls. at 30 min, i.e., the time of maxim ...	1976	1248454
renaturation of bacteriophage lambda dna. determination of the optimal renaturation conditions using a single-strand-specific dnase and alkaline-sucrose-gradient assay system.	reannealed hybrid molecules of wild-type bacteriophage lambda dna were prepared in aqueous solutions of formamide at a variety of nacl concentrations at both room temperature ( 22 degrees c) and 37 degrees c. treatment of the hybrid dna molecules with the single-strand-specific nuclease s1 from aspergillus oryzae followed by alkaline sucrose gradient sedimentation was used to monitor the extent and fidelity of hybridization. the optimal renaturation conditions at room temperature were found to b ...	1976	1248479
relationship between receptor and mammary tumour virus production after stimulation by glucocorticoid.	a short exposure of primary cultures of mouse mammary tumour cells to glucocorticoids results in at least a three-fold stimulation of mammary tumour virus (mtv) production. specific interaction of glucocorticoids with the cytoplasmic and nuclear receptors can also be demonstrated. the biological potency of various steroids to stimulate mtv is related directly to the retention of the steroid-receptor complex in the nuclei. progesterone has a high affinity for the cytoplasmic receptor, is not reta ...	1976	180430
topographical anatomy of human cerebral and cerebellar astrocytomas in vitro.	this study further characterizes the morphological and in vitro properties of human neoplastic cells derived from one grade iv cerebral astrocytoma and one grade iii cerebellar astrocytoma. light microscopic observations performed weekly over a four month period revealed two morphologically indistinguishable cell populations in each of the tumor cultures. scanning electron microscopy demonstrated the presence of microvilli, blegs, and ruffles on the cell surfaces of both tumors; however, these t ...	1976	188206
epstein-barr-virus-specific iga and igg serum antibodies in nasopharyngeal carcinoma.	the sera of 73 patients with nasopharyngeal carcinoma (npc), 28 patients with other carcinomas (oc) and 89 healthy subjects (hs) were tested for igg and iga antibodies to epstein-barr virus (ebv) viral capsid antigen (vca). the majority of the npc sera had igg titres of 160 or above, whereas the majority of the other sera had titres below 160. for iga reactivity to ebv-vca, 68 of 73 (93-2%) npc sera had titres of greater than or equal to 10. in contrast, only 6 of 28 (21-4%) oc sera and none of ...	1976	188437
naturally-occurring neonatal canine herpesvirus infection.	the detailed clinical and epidemiological history and the pathology of neonatal canine herpesvirus (chv) disease in a naturally infected litter of pups is reported. herpesvirus was isolated from the brain, lung, liver, spleen, kidney and intestine of one of the affected litter which died on day 10 after whelping. the isolation of virus correlated with the distinctive gross and histological changes in these organs. herpesvirus was isolated also from the anterior vagina of the bitch 18 days after ...	1976	189479
epstein-barr virus-specific iga serum antibodies as an outstanding feature of nasopharyngeal carcinoma.	stimulated by a report on elevated iga levels in nasopharyngeal carcinoma (npc), we tested a total of 372 sera from patients with npc, other carcinomas of head and neck or elsewhere, burkitt's lymphoma (bl), infectious mononucleosis (im) or healthy controls. the sera were titrated in indirect immunofluorescence tests for iga antibodies to epstein-barr virus (ebv) capsid antigen (vca) and to the diffuse (d) or restricted (r) components of the ebv-induced early antigen (ea) complex. the results pr ...	1976	175020
testicular protein kinases. characterization of multiple forms and ontogeny.	protein phosphokinase activity from the cytosol (105,000 x g soluble fraction) of testes from sexually mature rats has been resolved be deae-cellulose chromatography in three forms of protein kinase, camp-dependent protein kinases i and ii and camp-independent protein kinase iii. adenosine 3':5'-monophosphate-binding activity (camp-binding activity) was associated with protein kinases i and ii but not with protein kinase iii. protein kinases i, ii, and iii exhibited different ph optima, cyclic n ...	1976	175066
androgen and estrogen receptors in brain cytosol from male, female, and testicular feminized (tfm/y hermaphrodite) mice.	specific binding of [3h] 5alpha-dihydrotestosterone (dht) and [3h] estradiol by cytoplasmic extracts from whole brain of castrated male, female, and androgen-insensitive, testicular feminized (tfm/y male-female), mice has been investigated using glycerol gradient centrifugation and charcoal assay. mouse brain cytosol contains macromolecules with the characteristics of steroid hormone receptors, binding preferentially with high-affinity androgens or estrogens. both dht- and estradiol-receptor com ...	1976	179788
the effect of cyclic amp and prostaglandins on the fibrinolytic activity of mouse neuroblastoma cells.		1976	174561
cytochemical studies on sarcolemma: na+, k+-adenosine triphosphatase and adenylate cyclase.	two highly lead-sensitive atpases, na+,k+-atpase and adenylate cyclase, can be demonstrated cytochemically by the lead precipitation technique in briefly prefixed tissue, provided that the free pb2+ concentration in the incubation medium is kept below 0.1 mm by a heavy metal chelator. under conditions suitable for na+,k+-atpase activity precipitation of final reaction product (lead phosphate) at the sarcolemma of cardiac muscle is abolished by 0.1-1mm ouabain. in contrast, reaction product depos ...	1976	130656
biochemical and immunological characterization of deoxythymidine kinase of thymidine kinaseless hela cells biochemically transformed by herpes simplex virus type.	thymidine kinase (tk) from herpes simplex virus type 1 (hsv-1) biochemically transformed hela cells, purified by affinity chromatography, has been characterized with respect to its electrophoretic mobility, molecular weight, activation energy, substrate specificity, and immunological specificity. tk purified from hsv-1-transformed hela cells has the same electrophoretic mobility as tk purified from hela cells lytically infected with hsv-1. the sedimentation velocity of purified tk from transform ...	1977	193790
p1, p5-bis-(5'-adenosyl)pentaphosphate: is this adenylate kinase inhibitor substrate for mitochondrial processes?	1. p1, p5-bis-(5'-adenosyl) pentaphosphate (ap5-a) inhibits "soluble" adenylate kinase even when this enzyme is an integral part of the complete mitochondrion. the ki is 10(-5) m, i.e. about two orders of magnitude higher than the inhibitor contants determined for the purified adenylate kinase of rabbit muscle and an enzyme preparation separated from the mitochondrial intermembrane space. the weaker inhibitory effect is due to a lower accessibility of the enzyme. 2. as to be expected ap5a which ...	1977	201118
epstein-barr virus and cytomegalovirus antibodies in infectious mononucleosis.	a method has been evolved for the demonstration of epstein-barr virus (ebv) and cytomegalovirus (cmv) infection in 83 cases of infectious mononucleosis. serum samples were tested for ebv igm, anti-vca igg, anti-ebna, cmv igm and cmv igg antibodies. an acute-phase sample (or samples) and a convalescence sample were examined in each case, and in 44 cases an additional samples was examined 5-12 months after the illness. since the different antibodies showed characteristic differences in both titre ...	1977	201153
plasma amine oxidase activity in 136 normal rhesus monkeys: relationships between enzyme activity and age, sex and genetic factors.	normative data on plasma amine oxidase activity were obtained for four primate species, m. mulatta, m. arctoides, c. aethiops and h. sapiens. significant between-species differences in activity were observed. in a large sample of m. mulatta, no significant relationship between age and plasma amine oxidase activity was observed, but adult males had significantly higher enzyme activities than adult females.	1977	96261
alkylphthalides isolated from ligusticum wallichii franch and their in vitro inhibitory effect on rat uterine contraction induced by prostaglandin f2alpha.		1977	271195
peri-oral reflexes in the conscious monkey (macaca mulatta).		1978	105692
[some aspects relating to the aflatoxin generation during the self-heating of cereals].	in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ...	1978	98933
[a case of bacterial endocarditis due to neisseria mucosa (author's transl)].		1978	101614
[volatile fatty acids in gastrointestinal diseases in young infants].		1978	101958
clinical aspects on 64 cases of juvenile and adult listeriosis in sweden.	in 1958-74 altogether 64 cases of bacteriologically verified infections of listeria monocytogenes were diagnosed in sweden in children, aged more than 27 days, and in adults. immunosuppression predisposed to the disease. thus, many patients had co-existing disorders, such as leukemia and alcoholism. sixteen patients had been treated with corticosteroids, which were combined with cytostatic drugs in nine. meningoencephalitis was diagnosed in 52 patients and was fatal in 16. the clinical symptoms ...	1978	104552
effect of ultrasound on testicular electrolytes (sodium and potassium).	the testes of 50 rats were placed in a cup filled with water and received 1 w/cm2 of ultrasound for 15 min. fluid was collected from the seminiferous tubules and rete testis of the treated and control groups at 1, 8, 12, and 24 hr intervals. ultrasound increased the sodium concentration in the fluid of the seminiferous tubules, decreased the sodium concentration in the fluid of the rete testis, increased the potassium concentration in the fluid of the rete testis, and decreased the potassium con ...	1978	104674
thyroxine inactivation by starvation in cultured hepatocarcinoma cells, formation of reverse triiodothyronine.	the effect of starvation on thyroid hormone metabolism was studied in monkey hepatocarcinoma monolayer cultures. nonphenolic ring monodeiodination of thyroxine, 3, 5, 3'-triiodothyronine and 3, 3'-diiodothyronine was accelerated. since phenolic ring deiodination of 3, 3',5'-triiodothyronine (reverse t3) was unaffected, this metabolite accumulated in the medium during thyroxine metabolism. this suggests that increased serum reverse t3 in malnourished humans may be caused by enhanced deiodination ...	1978	105981
relationship of intracellular 3',5'-adenosine monophosphate accumulation to parathyroid hormone release from dispersed bovine parathyroid cells.		1978	85548
electron microscopic study of the rat cerebral cortex, impregnated by the golgi method.		1978	89643
the effect of bleomycin on rat bone marrow cells.	experiments were carried out to determine the cytogenetic effects of the antibiotic bleomycin (blm) in rats using the bone marrow system. a total of eighteen male and eighteen female sprague-dawley rats were injected with varying concentration of blm, over varying periods of time. the results revealed that at low concentrations blm showed no alteration in the ploidy or the morphology of rat chromosomes. this however, was not the case when the dose or administration period was increased.	1978	90585
increased plasma prolactin levels induced in rats by d-fenfluramine: relation to central serotonergic stimulation.	d-fenfluramine (7.5 and 10 mg/kg i.p.) and quipazine (10 and 20 mg/kg i.p.) increased plasma prolactin levels in male rats. metergoline (3 mg/kg p.o.) or p-chlorophenylalanine (100 mg/kg x 3, orally) pretreatment markedly blocked the prolactin-releasing effect of both d-fenfluramine and quipazine. this result suggests that the effect of these drugs on prolactin secretion could be mediated through a serotonergic mechanism. brain serotonin may thus exert a stimulatory role on prolactin secretion i ...	1978	149011
[changes in atpase activity of dog kidney nuclear membrane under the effect of polyenic antibiotics].	effect of amphotericin b and nistatin on atpase activity of dog kidney nuclear membranes is studied in vivo and in vitro. long-term intravenous injections of the antibiotics do not change the atpase activity of kidney nuclear membranes. however, short-term injections of polyenic antibiotics have some effect on atpase activity on nuclear membranes: amphotericin b, considerably activates the enzyme . in vitro incubation of isolated dog kidney nuclei with amphotericin and nistatin at concentrations ...	1978	149566
effect of d-amphetamine on tryptophan and other aromatic amino acids in brain.	the investigation examined the mechanism of the increase in brain tryptophan concentration of rats treated with d-amphetamine. certain well recognised influences upon brain tryptophan have been excluded as responsible. thus, the effect is not associated with changes in the plasma concentrations of nefa or free tryptophan. it is probably not due to a tryptophan-specific mechanism, because amphetamine increases the ratio of brain/plasma concentrations not only of tryptophan but also of tyrosine an ...	1978	149670
the presence of two (na+ + k+)-atpase inhibitors in equine muscle atp: vanadate nad a dithioerythritol-dependent inhibitor.	a potent inhibitor of (na+ + k+)-atpase activity was purified from sigma equine muscle atp by cation- and anion-exchange chromatography. the isolated inhibitor was identified by atomic absorption spectroscopy and proton resonance spectroscopy to be an inorganic vanadate. the isolated vanadate and a solution of v2o5 inhibit sarcolemma (na+ + k+)-atpase with an i50 of 1 micrometer in the presence of 1 mm ethyleneglycol-bis-(beta-aminoethylether)-n,n'-tetraacetic acid (egta), 145 mm nacl, 6mm mgcl2 ...	1978	150289
[effect of beryllium salts on the activity of sarcoplasmic reticulum ca2+,mg2+-dependent atp-ase].	in the presence of mg2+ and ca2+ ions beryllium compounds inhibit the ca2+, mg2+-dependent atpase activity and the transport of ca2+ in the sarcoplasmatic reticulum vesicles. the inhibition is reversible and concurrent with respect to the mg2+ ions. in the absence of the mg2+ ions an addition of beryllium compounds stimulates the atpase activity, the dependence of the degree of its stimulation on the beryllium compounds concentration is characterized by a curve with a maximum. on the membranous ...	1979	154413
myosin isoenzyme redistribution in chronic heart overload.	since the first observation by spann et al., it has become clear that in cardiac hypertrophy induced by a mechanical overloading, the velocity of shortening of the cardiac muscle (vmax) is reduced (see ref. 2 for review). most authors agree that this mechanical alteration is accompanied by a decrease in the ca2+-dependent atpase activity of myosin (see ref. 3 for review). the molecular basis of such changes was unknown because the structural modifications of the myosin molecule were ill-defined. ...	1979	91973
differences in the susceptibility of mhc and non-mhc mixed lymphocyte reactions to suppression by murine amnionic fluid and its components.	the ability of mouse amniotic fluid (maf), alpha-foetoprotein (afp) and maf depleted of afp (maf - afp) to suppress primary one-way mlr's was investigated. it was found that maf, afp and maf - afp were all suppressive of mlr's specific for mhc, k, d or i + s determinants. suppression was observed when either lymph node or spleen cells were used as the responder cells. nylon wool column passage of these cells did not significantly affect the immunosuppressive action of these substances. in contra ...	1979	93135
cocultivation as a tool for the detection of oncoviruses in childhood leukemia.		1979	94580
probing the program of gene expression utilized in early development.	somatic cell nuclei injected into amphibian oocytes enlarge, exchange protein with the surrounding cytoplasm, and actively synthesize rna for weeks. the message activity of the rnas synthesized within the oocytes can be detected as new proteins made a few days after the injection of somatic nucleic. the oocyte cytoplasm seems to reprogram the injected nuclei, allowing the expression only of those genes which are normally active in oocytes (de robertis and gurdon, 1977). genes which are unexpress ...	1979	95388
enzyme-catalyzed dna unwinding. mechanism of action of helicase iii.		1979	227887
from long-leggety beasties - good lord, deliver us.		1979	260080
the development of our present views on the molecular aspects of human blood group specificity.		1979	89773
antibodies in histoplasmosis detected by use of yeast and mycelial antigens in immunodiffusion and electroimmunodiffusion.		1979	89825
serum concentration of human alpha 2 hs glycoprotein during the inflammatory process: evidence that alpha 2 hs glycoprotein is a negative acute-phase reactant.	a nonspecific opsonin function has been ascribed to human alpha 2 hs glycoprotein. its serum level has been shown to be decreased in trauma patients. recent studies from this laboratory revealed a heterogeneity among the products obtained in the course of the preparation of the protein. to date, no definitive agreement existed with regard to a molecular homogeneous entity of alpha 2 hs glycoprotein (ba-alpha 2 glycoproteins). the purpose of the current work was to study the variations in serum l ...	1979	90057
adsorption, penetration, and uncoating of murine leukemia virus studied by using its reverse transcriptase.	a procedure using the virus-associated reverse transcriptase was developed for following the kinetics of adsorption, penetration, and uncoating of murine leukemia virus. viral adsorption to cell membrane was determined by assaying this enzyme activity in isolated debris of mechanically disrupted cells after infection with murine leukemia virus in the presence of actinomycin d. at 37 degrees c, viral adsorption proceeded at a high initial rate, but after 5 min of incubation with the virus, it gra ...	1979	90159
[improving the effectiveness of virological studies in influenza].		1979	90208
[detection of humoral antibodies to species-specific and group ornithosis antigens in the dynamics of the infection].	the time course of antibody production to group- and species-specific antigens of ornithosis agent was studied by complement fixation (cft) and hemagglutination-inhibition (hi) tests. in rabbits after a single intravenous inoculation of the ornithosis agent, antibody to the homologous species-specific antigen appeared in the peripheral blood 3--5 days after inoculation and reached maximum levels during the 1-st week, and to the group-specific antigen could be detected 3--5 days after inoculation ...	1979	90431
comparison of human alkaline phosphatase isoenzymes. structural evidence for three protein classes.	the structural relationships among human alkaline phosphatase isoenzymes from placenta, bone, kidney, liver and intestine were investigated by using three criteria. 1. immunochemical characterization by using monospecific antisera prepared against either the placental isoenzyme or the liver isoenzyme distinguishes two antigenic groups: bone, kidney and liver isoenzymes cross-react with anti-(liver isoenzyme) serum, and the intestinal and placental isoenzymes cross-react with the anti-(placental ...	1979	90505
murine autoimmune hemolytic anemia induced via xenogeneic erythrocyte immunization. ii. antigenic specificity and clonal heterogeneity of the autoantibodies and their cellular correlates.		1979	90574
study on dissimilatory reduction of sulphates.	a new strain of sulphate reducing bacteria was isolated from swampy forest soil. after 120 h reduction, sulphate conversion attained 100%, the molar ratio of the consumed lactate and reduced sulphate amounted to 2:1. this confirms the reduction mechanism proposed by senez (1951).	1979	87119
similarity of casein- and endotoxin-induced, myeloma- associated and aged sjl/j amyloid in various strains of mice.	amyloidosis was induced in a number of strains of mice by repeated injections of casein and endotoxin. spontaneous amyloid was obtained from balb/c mice bearing a myeloma tumor (igg2a producing mopc 173 tumor) and from aged sjl/j mice. both the induced and spontaneous forms were similar in their size, immunological reactivity, peptide maps and in the susceptibility of histological sections to oxidizing agents with or without trypsin digestion. since case-induced murine amyloid resembles the noni ...	1979	87374
immune suppression in vivo with antigen-modified syngeneic cells. ii. t cell-mediated nonresponsiveness to fowl gamma-globulin.	intravenous administration of syngeneic spleen cells coupled with the palmitoyl derivative of fowl gammma-globulin (p-f gamma g) results in a profound state of f gamma g-specific tolerance in c57bl/6 mice. administration of p-f gamma g coupled syngeneic cells specifically reduces both the primary and secondary hapten and carrier-specific pfc responses to tnp-f gamma g. since the haptenic response is affected, the tolerance functions at the level of the f gamma g-specific helper t cell. as few as ...	1979	87447
changes in seizure susceptibility, sleep time and sleep spindles following thalamic and cerebellar lesions.	the present experiment attempted to clarify conflicting evidence on the relationship of sleep spindles to seizure activation. seizure thresholds were calculated in minutes post-injection following ip administration of the convulsant drug monomethylhydrazine (mmh) to cats with lesions intended to alter the occurrence of spontaneous 12-15 c/sec sleep spindles recorded from sensorimotor cortex. twelve cats with bilateral cortical and subcortical recording electrodes were divided into 3 groups recei ...	1979	88323
passive transfer in diabetes mellitus.		1979	88629
[immunosuppression (author's transl)].	among different procedures of immunosuppression in man medical treatment with cytotoxic drugs has become the most important. biological methods are still under development but promise to gain in importance since they are more specific and have fewer side effects. the clinical effect of immunosuppressive therapy in organ transplantation is undisputed. its effects has been proven in some but not all immuno-inflammatory diseases. before immunosuppressive therapy is started it must be known whether ...	1979	88671
the pathophysiology of asthma.	because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ...	1979	89006
mac-1: a macrophage differentiation antigen identified by monoclonal antibody.		1979	89034
descending tracts of the lateral columns of the rat spinal cord: a study using the horseradish peroxidase and silver impregnation techniques.	the location of the cells of origin and the projection areas of descending fibre tracts of the spinal cord lateral columns were examined in rats. unilateral micro-transections of subpopulations of lateral column fibres, at c2 or t10, with subsequent application of horseradish peroxidase to the severed axons, allowed identification, by retrograde labelling, of those cell groups projecting to the spinal cord through the lateral columns. additionally, the pattern of fibre and preterminal degenerati ...	1979	89111
simulation and prevention of retrovirus--specific reactions by mycoplasmas.	from human mycosis fungoides tumor-derived cell lines, mycoplasma hyorhinis was isolated. this mycoplasma shared the following characteristics with retroviruses: uptake of 3h-uridine, but not of 3h-thymidine in cell culture; banding at 1.16 g/ml sucrose density and partial shift to retrovirus core density position (approximately equal to 1.24 g/ml) after detergent treatment; incorporation of 3h-tmp into high molecular weight material in standard reverse transcriptase assays with the template-pri ...	1979	89623
primary structure of a human iga1 immunoglobulin. iv. streptococcal iga1 protease, digestion, fab and fc fragments, and the complete amino acid sequence of the alpha 1 heavy chain.	in order to establish the complete amino acid sequence of the human iga alpha1 chain bur, iga1 protease from streptococcus sanguis was employed to generate fabalpha and fcalpha fragments in the final stage of this investigation. cyanogen bromide cleavage of the fabalpha fragment followed by reduction and aminoethylation produced the fd' fragment (residues 84 to 227); this contains part of the variable region (vr), the whole first constant domain (calpha1), and part of the hinge region of this he ...	1979	107164
electron microscopical study on gamogony of sarcocystis suihominis in human tissue cultures.	sexual stages and oocysts of sarcocystis suihominis were developed in human tissue cultures and studied with the electron microscope. this development was extremely rapid, being completed about 18--22 h post infection and there were no preceding schizogonic processes, thus confirming the earlier observations that schizogony is obligatorily restricted to the intermediate host in the sarcosporidian life cycle. micro- and macrogamonts could be distinguished about 12 h post infection and were situat ...	1979	107669
growth and food intake of prematurely weaned rats: effect of cortisone and thyroxine injection during the suckling period.		1979	108369
effects of morphine, diazepam and chlorpromazine on discrimination of electric shock.	the effects of morphine, diazepam and chlorpromazine were examined on the discrimination of electric shock in squirrel monkeys. two discrete responses measured discrimination of the presence or absence of shock at two different intensities, either at 0.35 ma or at an intensity determined to be close to each monkey's threshold (0.05 or 0.15 ma). the percentage of responses which were correct in the presence and the absence of shock were determined separately. time to respond to the presence or th ...	1979	108386
tuberculosis of the mandible presenting as 'lumpy jaw'.		1979	108413
identification of 15-keto-9, 11-peroxidoprosta-5, 13- dienoic acid as a hematin-catalyzed decomposition product of 15-hydroperoxy-9, 11-peroxidoprosta-5, 13-dienoic acid.	a labile prostaglandin was isolated as one of the products generated from [1-14c] eicosatetraenoic acid incubated with sheep vesicular gland microsomes. the eicosatetraenoic acid metabolite amounted to ca. 16% of the total radiolabeled products. formation of this new prostaglandin was prevented when heat-denatured microsomes were employed or when incubation mixtures were supplemented with indomethacin or phenol. however, incubation of prostaglandin g2 (pgg2) with hematin in the presence or absen ...	1979	108495
["in vitro" activity of a new cephalosporin (cgp-7174-e or cefsulodin) against "pseudomonas aeruginosa" (author's transl)].	the minimal inhibitory concentration (mic) of four betalactamins (carbenicillin, ticarcillin, piperacillin and (cgp-7174-e) against 109 clinical isolates of pseudomonas aeruginosa was determined by an agar dilution method. cgp-7174-e was the most active (mic 3.4 micrograms/ml), followed in descending order by piperacillin (mic 4.9 micrograms/ml), ticarcillin (mic 22 micrograms/ml) and carbenicillin (mic 36 microgram/ml). high correlation was found between the mic of these betalactamins (r greate ...	1979	120122
assessment of the efficacy of interventions to limit ischemic injury by direct measurement of intramural carbon dioxide tension after coronary artery occlusion in the dog.	although numerous interventions have been shown to exert a salutary effect on the ischemic myocardium, the severity of ischemia generally has been measured by indirect techniques. in the present investigation the effect of ischemia on intramural carbon dioxide tension (pmco(2)) was measured directly in the open-chest, anesthetized dog with a mass spectrometer during repetitive 10-min coronary artery occlusions separated by 45-min periods of reflow; simultaneously, regional myocardial blood flow ...	1979	105016
wits versus genes: the continuing battle against infection.		1979	105149
cell-mediated and humoral immune responses to cartilage antigenic components.	cell-mediated immunity and antibody production to cartilage antigens were studied in rabbits. from day 3 of immunization an inhibition of leucocyte migration was observed in animals immunized with collagen-free fractions of bovine nasal or human rib cartilage. delayed cutaneous hypersensitivity was elicited between 5 and 6 days and the circulating antibodies were demonstrated by passive haemagglutination on day 9. no significant correlation was observed between the antibody production and the ce ...	1979	105405
shedding of peripheral cytoplasm - a mechanism of liver cell atrophy in human amyloidosis.	a liver biopsy specimen from a case of primary amyloidosis was investigated by electron microscopy. the cytoplasmic periphery of the hepatocytes showed degenerativechanges which are interpreted as indicating shedding of peripheral parts of the cytoplasm. two main variants of this process could be discerned: 1) protrusion and sequestration of hernia-like blebs of cytoplasm, and 2) shedding of vesicles derived from degenerated endoplasmic reticulum. in the latter case transient defects of the plas ...	1979	105463
multiple sclerosis: immunochemical studies on the demyelinating serum factor.	sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ...	1979	105780
shunting of microspheres across the canine coronary circulation.	coronary shunting of 9 +/-1 micrometer and 25 +/- 5 micrometer radiolabeled microspheres was examined in anesthetized, open-chest dogs, whose left common coronary arteries were perfused at controlled pressures. shunting was estimated from the difference in radioactivity between perfusion line and coronary sinus blood samples during selective elevations of coronary perfusion pressure (cpp), left ventricular afterload, and inspired oxygen. a linear relationship was found between coronary shunting ...	1979	434177
a chronic cannula for obtaining csf from the cisterna magna of awake dogs.	we have designed a cannula system that can be chronically implanted to end above the dura of the cisterna magna of the dog. during experiments in the awake dog, a screw cap with stylet is removed from the cannula and a spinal needle inserted for the withdrawal of samples of cisternal cerebrospinal fluid (csf) or for making continuous measurements of pressure. the system can be used for repeated experiments extending over several weeks.	1979	434184
an xyy man.	this case report describes a young man with a 47, xyy karyotype who was convicted of arson. he suffered from a cardiac disorder which may well have been part of the xyy syndrome rather than a chance association. his abnormal karyotype was disclosed in court and used by the defence in a plea in mitigation.	1979	497632
interactions of folch-lees proteolipid apoprotein with planar lipid bilayers.	water-soluble folch-lees proteolipid apoprotein from bovine cns white matter induces a voltage-dependent conductance in black lipid membranes. na+ is required for the induced conductance change but the established conductance has very low ionic selectivity. the induced conductance fluctuates with a minimum amplitude of 10(-11)--10(-10) mho. the magnitude of the conductivity change is dependent on protein concentration and on the composition of lipid bilayers. at a fixed voltage the induced condu ...	1979	522128
[microionophoretic study of the mechanism of action of gamma-hydroxybutyrate].	the effect of gamma-hydroxybutyric acid (ghba) on the extracellularly registered spontaneous electrical activity of nervous cells of the rabbit brain sensomotor cortex was studied using the microionophoretic technique. ghba decreased the frequency of action potential in the majority of the neurons studied. a specific gamma-aminobutyric acid (gaba) blocking agent bicuculline prevented gbha inhibitory effect. ghba is suggested to interact with central gaba-receptors. the frequency of discharges in ...	1979	540145
subarachnoid space of the cns, nasal mucosa, and lymphatic system.	we have briefly reviewed the literature pertaining to the movement of tracer molecules and infectious organisms within the olfactory nerve. there is a body of evidence indicating that tracers placed in the csf will quickly move via the olfactory nerve to the nasal mucosa and then to the cervical lymph nodes. organic and inorganic tracer materials and organisms as diverse as viruses, a bacillus, and an amoeba, when placed in the nasal cavity, have been shown to move from the nasal mucosa via the ...	1979	85446
selective inhibition of hemolysin production by staphylococcus epidermidis and staphylococcus haemolyticus.	the effect of lincomycin on growth and hemolysin production by staphylococcus epidermidis and staphylococcus haemolyticus is presented. lincomycin at subinhibitory concentration, clearly inhibited hemolysin yield without appreciably altering final growth density. the cells of these two microbes previously grown in the presence of lincomycin showed very low hemolytic activities upon disruption. the mode of lincomycin action on hemolysin production is discussed.	1981	7198361
species identification and susceptibility to 17 antibiotics of coagulase-negative staphylococci isolated from clinical specimens.	a total of 299 isolates of gram-positive, catalase-positive, coagulase-negative cocci were isolated from a variety of specimens collected from patients at a large university hospital, and 281 (94%) were identified as staphylococci by established methods. using the scheme of kloos and schleifer, we determined the species of the coagulase-negative staphylococci. staphylococcus epidermidis was the cause of all bacteremias and the most commonly isolated species from bone, joint, and wound infections ...	1982	7068839
speciation and antibiotic susceptibility patterns of coagulase-negative staphylococci.	during a six month period, 191 isolates of coagulase-negative staphylococci from blood, urine, cerebrospinal fluid and heart valves were identified to species level and tested for antimicrobial susceptibility. seventy-one percent of isolates were staphylococcus epidermidis, 8% staphylococcus warneri, 7% staphylococcus hominis, 7% staphylococcus haemolyticus, 4% staphylococcus capitis, 2% staphylococcus saprophyticus and 1% staphylococcus cohnii. approximately 4% of isolates were felt to be assoc ...	1982	7173185
characterization of coagulase-negative staphylococci from urinary tract specimens.	species of coagulase-negative staphylococci isolated from urine specimens submitted from both inpatients and outpatients to the clinical microbiology laboratory of a teaching hospital were identified with a biotyping system, with species then correlated by clinical features and antimicrobial susceptibility. of 145 isolates, 102 (70%) were staphylococcus epidermidis, 24 (17%) were staphylococcus saprophyticus, 7 (4.7%) were staphylococcus haemolyticus, 4 (2.8%) were staphylococcus hominis, 3 (2.1 ...	1983	6833480
chemical and biological characterization of a gonococcal growth inhibitor produced by staphylococcus haemolyticus isolated from urogenital flora.	the purified antigonococcal substance produced by staphylococcus haemolyticus no. 7 has shown a broad antigonococcal spectrum and a narrow antibacterial spectrum. the inhibitor produced in vitro was also active in the guinea pig subcutaneous chamber. the inhibitor has shown hemolytic activity; the human, horse, and mouse erythrocytes were the most susceptible. hemolytic and antigonococcal activities were inhibited in the presence of phosphatidylcholine. the amino acid composition of the antigono ...	1984	6437979
hub colonization as the initial step in an outbreak of catheter-related sepsis due to coagulase negative staphylococci during parenteral nutrition.	a prospective study was carried out to determine the pathogenesis of coagulase negative staphylococci catheter-related sepsis during parenteral nutrition. forty-three catheters were cultured by semiquantitative and quantitative methods. the skin around the puncture site was cultured at the time of catheter removal and three segments of the catheter were cultured apart: the hub, the proximal subcutaneous segment, and the tip. skin cultures were negative (89%) or yielded different coagulase negati ...	1984	6441008
bacterial and mycoplasmal flora of the middle ear of laboratory rats with otitis media.	purulent otitis media was confirmed at necropsy in 35 laboratory rats, 14 of which had shown clinical signs of middle ear infection. the bacterial flora of both the affected and grossly normal middle ears was similar, often mixed, and comprised mainly of staphylococcus haemolyticus, pasteurella pneumotropica and corynebacterium spp. twenty-one isolates of mycoplasma spp. were recovered from the middle ears of 11 rats with purulent otitis media and one normal rat. fourteen of the isolates were fu ...	1984	6513509
identification, clinical distribution, and susceptibility to methicillin and 18 additional antibiotics of clinical staphylococcus isolates: nationwide investigation in italy.	a multicentric study of clinical staphylococcus isolates was performed by seven operative units working in different areas of italy. over a 6-month period, a total of 3,226 staphylococci, isolated from in- and outpatients, were identified and tested for antimicrobial susceptibility by a protocol agreed upon by all units. on the basis of their bacteriolytic-activity patterns and other conventional tests, the isolates were identified by lyogroups , which closely correlate with human staphylococcus ...	1984	6565709
heparinlike molecules with anticoagulant activity are synthesized by cultured endothelial cells.	cultured microvascular endothelial cells isolated from rat epididymal fat pads produce glycosaminoglycans that accelerate thrombin-antithrombin complex formation. the heparinlike nature of these macromolecules was established by complete destruction of their anticoagulant activity employing purified flavobacterium heparinase. only 15% of the biologic activity of these complex carbohydrates was expressed when the heparin binding domain on the protease inhibitor was chemically modified at the trp ...	1985	3970699
psychogenic colic in a horse.	a 4-year-old mixed-breed gelding was presented with colic-type behavior of 5 days' duration that included pawing, looking at the flank area, and kicking and biting at the abdomen and flank when it was eating hay or grain. medical evaluation did not reveal the cause of the "colic." during its stay in the hospital, the horse improved as regarded the colic-type behavior while eating, but resumed this behavior when it was able to see hay that it was not allowed to eat. it was determined that the per ...	1985	3972697
glycosaminoglycans of rat cerebellum: ii. a developmental study.	total and individual glycosaminoglycans (gags) were determined in rat cerebellum in tissue explants at various postnatal ages. the major constituents of gags were chondroitin sulfate (cs), hyaluronic acid (ha), and heparan sulfate (hs). dermatan sulfate (ds) and keratan sulfate (ks) could not be detected and therefore each amounts to less than 5% of all gags at all ages studied. ha was the prominent gag during postnatal development and only a minor constituent at adult ages, whereas cs was the p ...	1985	3973596
influence of housing conditions on pregnancy outcome in cynomolgus monkeys (macaca fascicularis).	female cynomolgus monkeys (macaca fascicularis) were kept under 3 different housing conditions: individually in type a cages (45 x 45 x 60 cm), individually in type b cages (70 x 70 x 100 cm) and as couples in type b cages. primigravida did not show early embryonic mortality, differing significantly from 11.5% early losses in multigravida. early embryonic mortality was not affected by housing condition. further reproductive failure rates did not differ significantly for primigravid (18.5%) and m ...	1985	3974197
workers' rights in occupational health.		1985	4014003
workers' rights in occupational health.		1985	4014003
labeling of cysteine-containing peptides with 2-nitro-5-thiobenzoic acid.	a method for specific labeling of cysteine-containing peptides has been developed using ellman's reagent, 5,5'-dithiobis(2-nitrobenzoic acid) (dtnb). prior to cleavage with proteases or chemical reagents, proteins are reacted with dtnb, resulting in the formation of a mixed disulfide between the protein sulfhydryl group and 2-nitro-5-thiobenzoic acid (tnb). the formation of the mixed disulfide introduces a chromophore, with an absorbance peak at 328 nm. by monitoring peptide maps generated by hp ...	1985	4014680