Publications

structure of ferredoxin i from azotobacter vinelandii.	the structure of azotobacter vinelandii ferredoxin i (av fdi, 106 amino acids) has been redetermined, based on x-ray diffraction data from tetragonal crystals of the native protein and two heavy atom derivatives. the current model differs greatly from the one previously reported and is in agreement with arguments based on various spectroscopic and other methods. the unit cell parameters are a = b = 55.62 a and c = 95.51 a, whereas the space group was found to be p4(1)2(1)2 instead of p4(3)2(1)2. ...	1988	3422475
the protein responsible for center a/b in spinach photosystem i: isolation with iron-sulfur cluster(s) and complete sequence analysis.	the 9 kda polypeptide from spinach photosystem i (ps i) complex was isolated with iron-sulfur cluster(s) by an n-butanol extraction procedure under anaerobic conditions. the polypeptide was soluble in a saline solution and contained non-heme irons and inorganic sulfides. the absorption spectrum of this iron-sulfur protein was very similar to those of bacterial-type ferredoxins. the amino acid sequence of the polypeptide was determined by using a combination of gas-phase sequencer and conventiona ...	1988	3049567
consecutive infected aneurysms caused by salmonella.	treatment of infected aneurysms consists of operation, which depends on the location and pathologic type, and antibiotic therapy, before and at least six weeks following operation. the authors present a case of two consecutive aneurysms occurring in a patient with salmonella infection. full recovery was obtained after surgical excision of the aneurysms, prolonged antibiotic therapy, and cholecystectomy.	1988	3067740
a new expression system for mammalian cells based on putative replicator sequences of the mouse and a truncated thymidine kinase gene.	we have constructed a new expression vector for mammalian cells. the vector contains a truncated tk gene for amplification under selective conditions, a sequence putatively supporting the replication of plasmid dna in eukaryotic cells (murine autonomously replicating sequence) and an expression cassette for the cdna to be studied. as a model cdna we have used that of human tissue-type plasminogen activator (t-pa). analysis of hirt supernatants and chromosomal dna from l cells, prepared six weeks ...	1988	3149608
model of a complex between the tetrahemic cytochrome c3 and the ferredoxin i from desulfovibrio desulfuricans (norway strain).	a three-dimensional model of an electron-transfer complex between the tetrahemic cytochrome c3 and the ferredoxin i from the sulfate-reducing bacterium desulfovibrio desulfuricans (norway strain) has been generated through computer graphics methods. the model is based on the known x-ray structure of the cytochrome and on a model of the ferredoxin that has been derived through computer graphics modeling and energy minimization methods, from the x-ray structure of the homologous ferredoxin from pe ...	1988	2847143
ecology of colorado tick fever.		1988	2849373
antiviral chemoprophylaxis for cytomegalovirus infection.		1988	2849602
crystal structure of clostridium acidi-urici ferredoxin at 5-a resolution based on measurements of anomalous x-ray scattering at multiple wavelengths.	the crystal structure of clostridium acidi-urici ferredoxin has been determined using multiple wavelength anomalous diffraction (mad) techniques at 5.0-a resolution. the electron density map shows striking similarity to a map of peptococcus aerogenes ferredoxin computed at the same resolution from the atomic coordinates reported by adman et al. (adman, e. t., sieker, l. c., and jensen, l. h. (1973) j. biol. chem. 248, 3987-3996). such similarity is expected from the high degree of identity betwe ...	1988	3192542
influence of inoculation dose, inoculation schedule, chicken age, and host genetics on disease susceptibility and development of resistance to eimeria tenella infection.	various parasite- and host-related factors influencing disease susceptibility and development of protective immunity against eimeria tenella infection were investigated in two inbred strains of chickens. chickens that received a primary inoculation of 10(3), 10(4), or 10(5) oocysts showed a significant reduction in packed cell volume and produced significantly more oocysts than chickens inoculated with fewer oocysts. younger chickens were as susceptible as older chickens to identical parasite do ...	1988	3264150
construction of orbital prostheses using the silicone pattern technique.		1988	279692
the crystal structure of the three-iron ferredoxin ii from desulfovibrio gigas.	the crystal structure of oxidized ferredoxin ii from the sulfate-reducing bacterium desulfovibrio gigas has been determined and refined at 1.7 a resolution. the folding of the polypeptide chain is similar to that of the 2[4fe-4s] ferredoxin in peptococcus aerogenes, except for an extended helical segment near the c-terminus. the single [3fe-4s] cluster in d. gigas is similar to a [4fe-4s] cluster, but lacks one fe atom and is coordinated to cys-8, -14 and -50. the side chain of cys-11 is not bou ...	1989	2920839
[anaerobic bacteria in chronic renal insufficiency: apropos of a case].	we relate a case of chronic renal insufficiency complicated by urinary anaerobic infection caused by bacteroides vulgatus and peptococcus asaccharolyticus.	1989	2489402
gaspak plus versus anaerocult a--two carbon dioxide/hydrogen systems for cultivation of anaerobes.	two disposable carbon dioxide/hydrogen gas-generating systems (gaspak plus and anaerocult a) were compared by assessing growth of obligate anaerobic bacteria. eighty strains representing 28 species of anaerobic bacteria commonly occurring at various body sites were seeded onto 4 brain heart chocolate agar plates using a spiral plater; and 1 plate each was subsequently incubated in 2 anaerocult a and 2 gaspak plus systems. bacterial growth was expressed as colony-forming units per ml (cfu/ml), re ...	1989	2496538
structure of [4fe-4s] ferredoxin from bacillus thermoproteolyticus refined at 2.3 a resolution. structural comparisons of bacterial ferredoxins.	the structure of a low-potential ferredoxin isolated from bacillus thermoproteolyticus has been refined by a restrained least-squares method. the final crystallographic r factor is 0.204 for 2906 reflections with f greater than 3 sigma f in the 6.0 to 2.3 a resolution range. the model contains 81 amino acid residues, one [4fe-4s] cluster, and 59 water molecules. the root-mean-square deviation from ideal values for bond lengths is 0.018 a, and the mean coordinate error is estimated to be 0.25 a. ...	1989	2600971
[oncogenes in human leukemia].	results of our study on the activation of n-ras oncogene by point mutation in human leukemia and myelodysplastic syndrome have been described in this article. point mutation was observed mainly on the 12th, 13th and 61st amino acid codon of ras genes. therefore, oligomers containing mutations at these codons were used as probes for dot blot analysis of dna derived from patient's bone marrow cells or leukemia cells. polymerase chain reaction technique was used to amplify the dna of ras genes cont ...	1989	2650633
antibody responses and protection in mice immunized with herpes simplex virus type 1 antigen immune-stimulating complex preparations.	the formation of immune-stimulating complexes (iscoms) obtained by mixing the glycoside quil a with an antigen preparation derived from herpes simplex virus type 1 (hsv-1)-infected cell cultures using a zwitterionic detergent is described. the hsv-1 antigen preparation incorporated into iscoms elicited significantly greater antibody responses in mice than the same preparation administered together with aluminium hydroxide gel, and provided complete protection against hsv-1 or hsv-2 lethal, syste ...	1989	2769234
[anaerobic bacteria and their role in recurrent tonsillitis].	the presence of anaerobic bacteria in 100 palatal tonsils obtained during the tonsillectomy, was evaluated in quality and in quantity. 17 species of anaerobic bacteria were found. the most frequent were: bacteroides melaninogenicus, peptostreptococcus anaerobius, peptostreptococcus intermedius, propionibacterium, veillonella parvula and peptococcus asaccharolyticus. in most evaluations their density was 10(5)-10(6) in gram of tonsil tissue, sometimes 10(7). its role in recurrent tonsils infectio ...	1990	1981606
demonstration of the heterogeneity of the kappa-opioid receptors in guinea-pig cerebellum using selective and nonselective drugs.	in guinea-pig cerebellum, saturation studies reveal that the nonselective opioid [3h]ethylketazocine has a binding capacity (r) of 6.79 pmol/g tissue which is similar to the sum of the individual r values of the mu-, delta- and kappa 1-selective opioids. conversely, the binding parameters of the nonselective opioid [3h]bremazocine are best-fitted to a two-site model (kd1 = 0.12 nm, r1 = 11.3 pmol/g tissue; kd2 = 6.03 nm, r2 = 9.09 pmol/g tissue) with an r total value of 20.3 pmol/g tissue which ...	1990	2164939
the biotin-dependent sodium ion pump glutaconyl-coa decarboxylase from fusobacterium nucleatum (subsp. nucleatum). comparison with the glutaconyl-coa decarboxylases from gram-positive bacteria.	membrane preparations of fusobacterium nucleatum grown on glutamate contain glutaconyl-coa decarboxylase at a high specific activity (13.8 nkat/mg protein). the enzyme was solubilized with 2% triton x-100 in 0.5 m nacl and purified 63-fold to a specific activity of 870 nkat/mg by affinity chromatography on monomeric avidin-sepharose. the activity of the decarboxylase was strictly dependent on na+ (km = 3 mm) and was stimulated up to 3-fold by phospholipids. the glutaconyl-coa decarboxylases from ...	1990	2244788
amino acid sequence and molecular modelling of a thermostable two (4fe-4s) ferredoxin from the archaebacterium methanococcus thermolithotrophicus.	the amino acid sequence of a two (4fe-4s) ferredoxin from the methanogenic bacterium methanococcus thermolithotrophicus (fdmt) has been determined. this thermostable protein comprises 60 amino acid residues (mr 6541) and two (4fe-4s) clusters chelated to the protein through the eight cysteines. fdmt contains a relatively high number of lysines [5], threonines [4] and valines [10]. the three-dimensional molecular model generated from the peptococcus aerogenes x-ray structure keeps the characteris ...	1991	1986796
purification and properties of an iron-sulfur-containing and pyridoxal-phosphate-independent l-serine dehydratase from peptostreptococcus asaccharolyticus.	l-serine dehydratase with a specific activity of 15 nkat/mg protein was present in the anaerobic eubacterium peptostreptococcus asaccharolyticus grown either on l-glutamate or l-serine. the enzyme was highly specific for l-serine with the lowest km = 0.8 mm ever reported for an l-serine dehydratase. l-threonine (km = 22 mm) was the only other substrate. v/km for l-serine was 500 times higher than that for l-threonine. l-cysteine was the best inhibitor (ki = 0.3 mm, competitive towards l-serine). ...	1991	2065681
two-dimensional nmr investigation of iron-sulfur cluster electronic and molecular structure of oxidized clostridium pasteurianum ferredoxin. interpretability of contact shifts in terms of cysteine orientation.	a two-dimensional nmr study has been carried out on the four-iron clusters of a bacterial oxidized ferredoxin for the purpose of investigating the relationship between contact shift patterns and the orientation of the individual coordinated cysteines. the ferredoxin from clostridium pasteurianum, cpfdox, was selected because of its extensive sequence homology, and likely close structural similarity, to the crystallographically characterized ferredoxin from peptococcus aerogenes, pa fdox (adman, ...	1991	1748648
comparative in vitro activity of cefpodoxime against anaerobes other than bacteroides fragilis.	to assess the in vitro activity of cefpodoxime against anaerobic respiratory tract and oropharyngeal pathogens 77 strains belonging to 18 gram-negative and 7 gram-positive species were studied by means of agar dilution tests. for comparison cefuroxime, amoxicillin, amoxicillin + clavulanic acid and clindamycin were also tested. cefpodoxime was found to be active at concentrations of less than or equal to 0.125 mg/l against prevotella oralis, prevotella buccalis, prevotella bivia, porphyromonas a ...	1991	1800380
bidirectional morphological changes induced by dexamethasone in morris hepatoma cell lines mca-rh7777 and mca-rh8994: independence of fibronectin and its receptor.	two morris hepatoma-derived cell lines, mca-rh7777 (7777) and mca-rh8994 (8994), exhibit different alterations in morphology upon exposure to glucocorticoid. after treatment with synthetic glucocorticoid dexamethasone (dex), 7777 cells show increased adhesiveness and more flattened shape, while dex-treated 8994 cells show decreased adhesiveness to substratum and exhibit a marked increase of round and detached cells. since fibronectin has been thought to play an important role in cell adhesivenes ...	1991	1829041
selection, expression, and nucleotide sequencing of the glutamate dehydrogenase gene of peptostreptococcus asaccharolyticus.	the gene for the catabolic nad-linked glutamate dehydrogenase of peptostreptococcus asaccharolyticus was cloned by selection of escherichia coli for complementation of a biosynthetic defect. cloned fragments containing the gene and the p. asaccharolyticus transcription and translation signals are very highly expressed in e. coli. the nucleotide sequence of the cloned gene was determined. it codes for a polypeptide of 421 amino acids, the sequence of which is similar to those of the nadp-acceptin ...	1991	1917850
the partial amino acid sequence of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum: implications for the evolution and structural basis of coenzyme specificity.	the amino acid sequence is reported for cnbr and tryptic peptide fragments of the nad(+)-dependent glutamate dehydrogenase of clostridium symbiosum. together with the n-terminal sequence, these make up about 75% of the total sequence. the sequence shows extensive similarity with that of the nadp(+)-dependent glutamate dehydrogenase of escherichia coli (52% identical residues out of the 332 compared) allowing confident placing of the peptide fragments within the overall sequence. this demonstrate ...	1991	1954226
visualization and rapid quantification of autoradiographic labeling in scanning electron microscopy applied to localization of receptor sites on the surface of whole cells.	the use of backscattered electron imaging (bei) as a routine procedure for examining autoradiographic reactions in scanning electron microscopy (sem) is described. this technique allows the determination of the number of receptor sites occupied by 125i-epidermal growth factor (egf) on whole cells. the effect of 1.25 dihydroxyvitamin d3 (1,25 (oh)2d3) on the number of epidermal growth factor receptors (egf-r) in the bt 20 human mammary carcinoma cell line (which is known to possess a very high nu ...	1992	1360725
[asporogenic anaerobic thoracic empyema--an experimental model for anaerobic thoracic empyema in rabbits].	sterile pleural effusion was induced by intrapleural administration of turpentine, and empyema was induced by direct intrapleural inoculation of bacteria in rabbits. experimental models of anaerobic thoracic empyema were successfully made in this study, using escherichia coli, peptostreptococcus asaccharolyticus, bacteroides fragilis, using the following methods; (1) a single bacterium inoculation of, a) e. coli, b) p. asaccharolyticus, c) b. fragilis, (2) simultaneous inoculations with an aerob ...	1992	1402083
protein control of iron-sulfur cluster redox potentials.	the relationship between the three-dimensional structures of iron-sulfur proteins and the redox potentials of their iron-sulfur clusters is of fundamental importance. we report calculations of the redox potentials of the [fe4s4(s-cys)4]-2/-3 couple in four crystallographically characterized proteins: azotobacter vinelandii ferredoxin i, peptococcus aerogenes ferredoxin, bacillus thermoproteolyticus ferredoxin, and chromatium vinosum high potential iron protein (hipip). our calculations use the " ...	1992	1464583
2,6-dichlorophenolindophenol is a competitive inhibitor for xanthine oxidase and is therefore not usable as an electron acceptor in the fluorometric assay.	xanthine oxidase has been recognized as an important source of oxygen free radicals in ischemia-reperfusion injury. in order to study this enzyme in biological tissues, the conversion of pterin (2-amino-4-hydroxypteridine) to isoxanthopterin provides the basis for a very sensitive fluorometric assay. xanthine oxidase is typically assayed in the presence of pterin only, while an electron acceptor which replaces nad+ is used to determine the combined xanthine dehydrogenase plus xanthine oxidase ac ...	1992	1563644
yeast telomere repeat sequence (trs) improves circular plasmid segregation, and trs plasmid segregation involves the rap1 gene product.	telomere repeat sequences (trss) can dramatically improve the segregation of unstable circular autonomously replicating sequence (ars) plasmids in saccharomyces cerevisiae. deletion analysis demonstrated that yeast trss, which conform to the general sequence (c(1-3)a)n, are able to stabilize circular ars plasmids. a number of trs clones of different primary sequence and c(1-3)a tract length confer the plasmid stabilization phenotype. trs sequences do not appear to improve plasmid replication eff ...	1992	1569937
the iron-sulfur-cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus. stereochemistry of the deamination of l-threonine.	the stereochemistry of the deamination of l-threonine to 2-oxobutyrate, catalyzed by purified l-serine dehydratase of peptostreptococcus asaccharolyticus, was elucidated. for this purpose the enzyme reaction was carried out with unlabelled l-threonine in 2h2o and in 3hoh, as well as with l-[3-3h]threonine in unlabelled water. isotopically labelled 2-oxobutyrate thus formed was directly reduced in a coupled reaction with l- or d-lactate dehydrogenase and nadh. the (2r)- or (2s)-2-hydroxybutyrate ...	1992	1572371
transforming growth factor beta induces myoblast differentiation in the presence of mitogens.	transforming growth factor beta 1 (tgf-beta 1) added to l6e9 rat skeletal myoblasts in mitogen-rich medium induces a rapid decrease in c-myc expression and delays progression through the g1 phase of the cell cycle. this growth inhibitory response is followed by cell commitment to terminal differentiation with elevated expression of myogenin muscle determination genes, induction of muscle-specific proteins, and formation of multinucleated myotubes. these results suggest that tgf-beta 1 may act as ...	1992	1594628
bacterial l-serine dehydratases: a new family of enzymes containing iron-sulfur clusters.	two families of enzymes are described which catalyse identical chemical reactions but differ in their prosthetic groups and hence in their mechanism of action. one family, the pyridoxal-5'-phosphate (plp)-dependent l-threonine dehydratases, also use l-serine as substrate. the other, hitherto unrecognized family is the iron-dependent, highly specific bacterial l-serine dehydratases. it has been shown that l-serine dehydratase from the anaerobic bacterium peptostreptococcus asaccharolyticus contai ...	1993	8236444
[gardnerella vaginalis in infections of reproductive organs].	the study was aimed at multidirectional studies on bacteria isolated from smears from vagina and cervix uteri in 226 patients with inflammatory states of their reproductive organs. most frequently isolated aerobic bacteria were gram-negative--27%, mainly e. coli, and enterococcus faecalis--in 18% of cases. anaerobic bacteria were isolated in 81% of cases: gardnerella vaginalis was isolated in 28% and lactobacillus in 53% of cases. other anaerobic bacteria were: peptococcus asaccharolyticus (15.5 ...	1993	8309297
peritonsillar abscess, retropharyngeal abscess, mediastinitis, and nonclostridial anaerobic myonecrosis: a case report.	peritonsillar abscess is a potentially life-threatening complication of acute tonsillitis. on occasion, peritonsillar abscess can extend to neck spaces and/or to the mediastinum. we describe a case of a patient with a peritonsillar abscess that extended to the neck, producing bilateral retropharyngeal abscesses and myonecrosis of the strap muscles. culture of a specimen of the necrotic muscle yielded prevotella intermedia, prevotella buccae, lactobacillus catenaforme, another lactobacillus speci ...	1993	8324135
l-serine and l-threonine dehydratase from clostridium propionicum. two enzymes with different prosthetic groups.	l-serine dehydratase from the gram-positive bacterium peptostreptococcus asaccharolyticus is novel in the group of enzymes deaminating 2-hydroxyamino acids in that it is an iron-sulfur protein and lacks pyridoxal phosphate [grabowski, r. and buckel, w. (1991) eur. j. biochem. 199, 89-94]. it was proposed that this type of l-serine dehydratase is widespread among bacteria but has escaped intensive characterization due to its oxygen lability. here, we present evidence that another gram-positive ba ...	1993	8344301
in vitro activity of fk037, a new parenteral cephalosporin, against anaerobic bacteria.	the activity of fk037, a new parenteral cephalosporin, was compared with those of cefpirome, ceftazidime, and flomoxef against 322 recent clinical isolates of anaerobic bacteria. a fastidious facultative anaerobe, gardnerella vaginalis, was also studied. fk037 inhibited 90% of isolates of peptostreptococcus anaerobius, peptostreptococcus asaccharolyticus, clostridium perfringens, mobiluncus spp., g. vaginalis, and porphyromonas gingivalis at < or = 0.78 micrograms/ml. the mics of fk037 for 50 an ...	1993	8517721
structure at ph 6.5 of ferredoxin i from azotobacter vinelandii at 2.3 a resolution.	ferredoxin i from azotobacter vinelandii (avfdi) is an iron-sulfur protein composed of 106 amino acids, seven fe atoms and eight inorganic s* atoms. a crystallographic redetermination of its structure showed the originally reported structure to be incorrect. we report here the crystal structure of avfdi at ph 6.5. extensive refinement has led to a final r value of 0.170 for all 6986 non-extinct reflections in the range 10-2.3 a using a solvent model which includes 98 discrete solvent atoms with ...	1993	15299532
crystal structure of the ferredoxin i from desulfovibrio africanus at 2.3 a resolution.	the crystal structure of the ferredoxin i from the sulfate-reducing bacterium desulfovibrio africanus (dafdi) has been solved and refined by x-ray diffraction. the crystals are orthorhombic with a = 96.6 a, b = 58.1 a, and c = 20.7 a, space group p2(1)2(1)2, and two ferredoxin molecules per asymmetric unit. the initial electron density map has been obtained by combining phasing by molecular replacement methods, anomalous scattering, and noncrystallographic averaging. the final crystallographic r ...	1994	7803404
comparison of native and mutant proteins provides a sequence-specific assignment of the cysteinyl ligand proton nmr resonances in the 2[fe4s4] ferredoxin from clostridium pasteurianum.	a sequence-specific assignment is presented for the eight low-field paramagnetically shifted cysteinyl ligand proton nmr resonances in the 2[fe4s4] ferredoxin from clostridium pasteurianum. the assignment is based upon comparison of chemical shifts in 1d and 2d nmr spectra of native oxidized protein and those of three mutants. the mutant proteins g12a and g41a were designed to produce minor local structural changes (hence small chemical shift perturbations) in either cluster i (glycine 12 to ala ...	1994	7981209
iron-sulfur cluster-containing l-serine dehydratase from peptostreptococcus asaccharolyticus: correlation of the cluster type with enzymatic activity.	investigations were performed with regard to the function of the iron-sulfur cluster of l-serine dehydratase from peptostreptococcus asaccharolyticus, an enzyme which is novel in the class of deaminating hydro-lyases in that it lacks pyridoxal-5'-phosphate. anaerobically purified l-serine dehydratase from p. asaccharolyticus revealed epr spectra characteristic of a [3fe-4s]+ cluster constituting 1% of the total enzyme concentration. upon incubation of the enzyme under air the intensity of the [3 ...	1994	8082806
calculation of the redox potentials of iron-sulfur proteins: the 2-/3-couple of [fe4s*4cys4] clusters in peptococcus aerogenes ferredoxin, azotobacter vinelandii ferredoxin i, and chromatium vinosum high-potential iron protein.	calculations of the redox potentials of the 2-/3-couples of [fe4s*4cys4] clusters in the iron-sulfur proteins peptococcus aerogenes ferredoxin (pafd), azotobacter vinelandii ferredoxin i (avfdi) and chromatium vinosum high potential iron protein (cvhipip) based on the protein dipoles langevin dipoles (pdld) method are reported. the structures of these proteins have been determined by x-ray crystallography; in the case of pafd the structure has recently been revised due to a change in the sequenc ...	1994	8086408
16s ribosomal dna sequences of anaerobic cocci and proposal of ruminococcus hansenii comb. nov. and ruminococcus productus comb. nov.	the 16s ribosomal dna sequences of representative members of the family peptococcaceae were determined. the members of the family examined were divided into the following four phylogenetic groups: peptococcus niger atcc 27731t (t = type strain), the sarcina-peptostreptococcus anaerobius group, the ruminococcus-coprococcus group, and the peptostreptococcus group. peptococcus niger, the type species of the family, was not related to other members of the family. peptostreptococcus anaerobius atcc 2 ...	1994	8123556
azotobacter vinelandii ferredoxin i. alteration of individual surface charges and the [4fe-4s]2+/+ cluster reduction potential.	the structures of azotobacter vinelandii ferredoxin i (avfdi) and peptococcus aerogenes ferredoxin (pafd), near their analogous [4e-4s]2+/+ clusters, are highly conserved (backes, g., mino, y., loehr, t.m., meyer, t.e., cusanovich, m.a., sweeney, w.v., adman, e.t., and sanders-loehr, j. (1991) j. am. chem. soc. 11, 2055-2064). despite these similarities, the reduction potential (e0') of the avfdi [4fe-4s]2+/+ cluster is more than 200 mv more negative than that of pafd. we have tested the contrib ...	1994	8132582
techniques for controlling variability in gram staining of obligate anaerobes.	identification of anaerobes recovered from clinical samples is complicated by the fact that certain gram-positive anaerobes routinely stain gram negative; peptostreptococcus asaccharolyticus, eubacterium plautii, clostridium ramosum, clostridium symbiosum, and clostridium clostridiiforme are among the nonconformists with regard to conventional gram-staining procedures. accurate gram staining of american type culture collection strains of these anaerobic bacteria is possible by implementing fixin ...	1995	7538512
the in vitro activity of trospectomycin against anaerobic bacteria.	the authors evaluated the activity of trospectomycin, a new aminocyclitol which is characterized by good antibacterial and broad spectrum activity, in comparison with clindamycin and ampicillin on a sample of recent isolates: bacteroides fragilis (15 strains), bacteroides urealyticus (5 strains), bacteroides vulgatus (5 strains), bacteroides spp. (15 strains), prevotella melaninogenica (6 strains), porphyromonas asaccharolytica (7 strains), mobiluncus spp. (3 strains), peptococcus niger (3 strai ...	1995	8667035
site-specific mutagenesis of rhodobacter capsulatus ferredoxin i, fdxn, that functions in nitrogen fixation. role of extra residues.	one of the two [4fe-4s]-type clusters of the rhodobacter capsulatus ferredoxin i, fdxn, was modified through site-specific mutagenesis of the distinctive features of the second cluster-binding motif, cys38-x2-cys41-x8-cys50-x3-cys54-x4-cys59. first, various mutagenized products were tested to learn whether they could rescue the decreased capacity of an fdxn-null strain msa1 to fix nitrogen: the phenotype of msa1 was reassessed to nifs (slow growth by nitrogen fixation) from our previous descript ...	1996	8940149
[the detection of peptostreptococcus asaccharolyticus and bacillus cereus in clinical materials from a child who died of sepsis with a fulminant clinical course].	a case of an acute disease with a rapid clinical course and a fatal outcome in the presence of irreversible toxicoinfectious shock, appearing in two children after the consumption of sheep kidneys, is described. the post mortem examination of the children revealed the presence of hemorrhagic, erosive and necrotic areas on the mucous membrane of the stomach, the duodenum and the upper section of the small intestine. from the material obtained by probing the stomach of one of the children 6 hours ...	1996	9103069
antibacterial activities of tosufloxacin against anaerobic bacteria and the electron micrograph of its bactericidal effects.	tosufloxacin, a quinolone, showed a broad antibacterial spectrum against gram-positive and gram-negative bacteria including anaerobic bacteria. tosufloxacin was 4- to 8-fold more active than levofloxacin and ciprofloxacin. the mic90 of tosufloxacin for clinical isolates of bacteroides fragilis, bacteroides vulgatus, bacteroides thetaiotaomicron and peptostreptococcus asaccharolyticus were 0.78, 0.39, 1.56 and 0.39 micrograms/ml, respectively. morphological observation with the scanning and trans ...	1997	9142454
y13c azotobacter vinelandii ferredoxin i. a designed [fe-s] ligand motif contains a cysteine persulfide.	ferredoxins that contain [4fe-4s]2+/+ clusters often obtain three of their four cysteine ligands from a highly conserved cysxxcysxxcys sequence motif. little is known about the in vivo assembly of these clusters and the role that this sequence motif plays in that process. in this study, we have used structure as a guide in attempts to direct the formation of a [4fe-4s]2+/+ in the [3fe-4s]+/0 location of native (7fe) azotobacter vinelandii ferredoxin i (avfdi) by providing the correct three-dimen ...	1997	9188450
cloning and expression of the two genes coding for l-serine dehydratase from peptostreptococcus asaccharolyticus: relationship of the iron-sulfur protein to both l-serine dehydratases from escherichia coli.	the structural genes sdha and sdhb, coding for the alpha- and beta-subunits of the [4fe-4s] cluster containing l-serine dehydratase from peptostreptococcus asaccharolyticus, have been cloned and sequenced. expression of modified sdhb together with sdha in escherichia coli led to overproduction of active his6-tagged l-serine dehydratase. e. coli mew22, deficient in the l-serine dehydratase l-sd1, was complemented by this sdhba construct. the derived amino acid sequence of sdhba shares similaritie ...	1997	9244285
aminopeptidase activities in peptostreptococcus spp. are statistically correlated to gelatin hydrolysis.	one hundred peptostreptococcus isolates from five species were assessed for their ability to hydrolyze gelatin. most peptostreptococcus magnus (95.8%) and peptostreptococcus micros isolates (79.0%) hydrolyzed gelatin in contrast to peptostreptococcus asaccharolyticus (8.0%), peptostreptococcus anaerobius (10.0%), and peptostreptococcus prevotii isolates (16.7%). gelatin hydrolysis in peptostreptococcus magnus and peptostreptococcus micros isolates correlated (r = 0.80; p = 0.0019) with more amin ...	1998	9606913
partial purification of an iron-dependent l-serine dehydratase from clostridium sticklandii.	an oxygen-sensitive and highly unstable l-serine dehydratase was partially purified from the gram-positive anaerobe clostridium sticklandii. the final active preparation contained five proteins of 27, 30, 44.5, 46, and 58 kda as judged by sds-page. the n-terminal sequence of the 30 kda subunit showed some similarity to the alpha-subunits of the iron-containing l-serine dehydratases from clostridium propionicum and peptostreptococcus asaccharolyticus. oxygen-inactivated l-serine dehydratase from ...	1998	9637015
chloramphenicol and tetracycline usage in outpatient practice.		1998	121377
microstimulation of precentral cortex with chronically implanted microelectrodes.		1998	107037
[c-peptide, igg-insulin antibodies, island cell antibodies and immune response genes in diabetes mellitus].		1998	105488
the saodap cooperative studies of laam: unblinded comparison with methadone.		1998	106292
continuous subcutaneous insulin infusion or subcutaneous insulin injections.		1999	89550
plasma 1,25(oh)2 vitamin d concentrations in nutritional osteomalacia.		1999	89299
atypical phenylketonuria caused by 7, 8-dihydrobiopterin synthetase deficiency.	a patient with atypical phenylketonuria and normal liver dihydropteridine reductase and phenylalanine-4-hydroxylase activities excreted neopterin but not biopterin or dihydrobiopterin in urine. the oral administration of l-sepiapterin (1 mg/kg body weight) lowered serum-henylalanine from 17.1 to 1.1 mg/dl within 6 h. comparable responses were observed after oral administration of l-erythro-7, 8-dihydrobiopterin or l-erythro-5, 6, 7, 8-tetrahydrobiopterin (each given in a dose of 2.5 mg/kg body w ...	1999	84153
vitiligo patterns simulating autoimmune and rheumatic diseases.	several variants of generalised vitiligo can be recognised by their cutaneous distribution. in some, certain anatomical regions--e.g., hands--are predominantly affected while the cutaneous depigmentation in other variants shows a similar anatomical distribution to that of the internal structures affected in some of the autoimmune and rheumatic diseases. five patients have been seen, and three described by others, with vitiligo affecting skin of eyelids and lower front of neck, reminiscent of the ...	1999	89452
deaths of infants after triple vaccine.		1999	89408
effect on bone of withdrawal of oestrogen therapy.		1999	89300
fatal leishmaniasis in renal-transport patient.		1999	88649
conception in a phenylketonuric woman.		1999	84971
in vitro activity of gemifloxacin (sb 265805) against anaerobes.	gemifloxacin mesylate (sb 265805), a new fluoronaphthyridone, was tested against 359 recent clinical anaerobic isolates by the national committee for clinical laboratory standards reference agar dilution method with supplemented brucella blood agar and an inoculum of 10(5) cfu/spot. comparative antimicrobials tested included trovafloxacin, levofloxacin, grepafloxacin, sparfloxacin, sitafloxacin (du-6859a), penicillin g, amoxicillin clavulanate, imipenem, cefoxitin, clindamycin, and metronidazole ...	1999	10471570
activities of gemifloxacin (sb 265805, lb20304) compared to those of other oral antimicrobial agents against unusual anaerobes.	the activities of gemifloxacin (sb 265805, lb20304) and comparator agents were determined by an agar dilution method against 419 clinical strains of less-commonly identified species of anaerobes. gemifloxacin was generally more active than trovafloxacin against gram-positive strains by one to two dilutions. peptostreptococci (peptostreptococcus asaccharolyticus, peptostreptococcus magnus, peptostreptococcus micros, and peptostreptococcus prevotii) and porphyromonas spp. (porphyromonas asaccharol ...	1999	10543754
response of the central metabolism in corynebacterium glutamicum to the use of an nadh-dependent glutamate dehydrogenase.	the extensive use of 13c enrichments in precursor metabolites for flux quantification does not rely on nadph stoichiometries and can therefore be used to quantify reducing power fluxes. as an application of this concept, the nadph fluxes were quantified in an l-lysine producer of corynebacterium glutamicum grown into metabolic and isotopic steady state with [1-13c]glucose. in this case, where the organism's nadph-dependent glutamate dehydrogenase consumes reducing power, the nadph flux generated ...	1999	10935753
synergic activity, for anaerobes, of trovafloxacin with clindamycin or metronidazole: chequerboard and time-kill methods.	chequerboard titrations were used to test the activity of trovafloxacin, alone and in combination with clindamycin or metronidazole, against 156 gram-positive or gram-negative anaerobes, including 47 bacteroides fragilis group, 36 prevotella spp., 26 fusobacteria, 21 peptostreptococci and 26 clostridia. mic50/mic90 values (mg/l) of each drug alone against all 156 strains were: trovafloxacin, 0.5/1; clindamycin, 0.25/2; metronidazole, 1/2. synergy (fic indices </= 0.5) was seen in two strains wit ...	2000	10797085
induction of human immunodeficiency virus type 1 expression by anaerobes associated with bacterial vaginosis.	bacterial vaginosis (bv) is a common disorder characterized by increased levels of anaerobic bacteria in the genital tract. bv has been associated with an increased rate of sexual transmission of human immunodeficiency virus (hiv). the effects of bv-associated anaerobic bacteria on hiv expression in monocytoid cells and t cells were examined. peptostreptococcus asaccharolyticus and prevotella bivia stimulated hiv expression in monocytoid cells, whereas bacteroides ureolyticus, peptostreptococcus ...	2000	10823756
clomipramine and clonazepam in cataplexy.		2000	91833
[evaluation of carbon dioxide laser surgical unit in neurosurgery (author's transl)].		2000	91992
poison ivy (rhus) dermatitis.		2001	150451
[nuclear surface of buccal cells as a function of the nature of the stain. measurements with an image analyzing computer].	measure of nuclear areas was performed on buccal smears by the quantimet 720 image analysing computer. five different staining procedures were used and we consider that toluidin blue at ph 4 is the best for a fine quantitative caryometry.	2001	88973
discovery of an inherited bisatellited metacentric microchromosome in amniotic cell culture.	the identification is reported of an extra bisatellited metacentric microchromosome in amniotic cell culture from the third pregnancy of an identical twin (amniocentesis being performed because of age), and its subsequent finding in the maternal parent as an inherited familial marker. the carriers of the microchromosome are all clinically normal and the parents opted for continuation of pregnancy. only one other report was found in the literature of a similar microchromosome detected in amniotic ...	2001	90568
peptostreptococcus asaccharolyticus renal abscess: a rare cause of fever of unknown origin.	renal abscess is uncommon in pediatrics and is rarely a cause of fever of unknown origin. we recently cared for a patient who presented with a 3-week history of fever. an indium scan ultimately led to the diagnosis of a renal abscess. aspiration yielded peptostreptococcus asaccharolyticus. this unusual case prompted a review of the clinical and microbiologic features of renal abscess in pediatric patients at our hospital over the past 10 years. seven additional patients with a discharge diagnosi ...	2001	11134475
microbial analysis of bone collected during implant surgery: a clinical and laboratory study.	dental implant surgery produces bone debris which can be used to correct bone defects in the "simultaneous-augmentation" technique. however, this debris is potentially contaminated with oral bacteria. therefore, this study examined bone debris collected during dental implant surgery in order 1) to identify the microbial contaminants and 2) to compare the effects of two different aspiration protocols on the levels of microbial contamination. twenty-four partially dentate patients were randomly al ...	2001	11251657
proposal of the genera anaerococcus gen. nov., peptoniphilus gen. nov. and gallicola gen. nov. for members of the genus peptostreptococcus.	members of genus peptostreptococcus have previously been found to be distantly related to the type species, peptostreptococcus anaerobius, on the basis of 16s rdna sequence similarities. they were divided into three major phylogenetic groups, and their peptidoglycan structure and biochemical traits differed between groups. the reclassification of the species of these three groups into three new genera, peptoniphilus gen. nov., anaerococcus gen. nov. and gallicola gen. nov., is proposed. the genu ...	2001	11491354
azotobacter vinelandii ferredoxin i: a sequence and structure comparison approach to alteration of [4fe-4s]2+/+ reduction potential.	the reduction potential (e(0)') of the [4fe-4s](2+/+) cluster of azotobacter vinelandii ferredoxin i (avfdi) and related ferredoxins is approximately 200 mv more negative than the corresponding clusters of peptostreptococcus asaccharolyticus ferredoxin and related ferredoxins. previous studies have shown that these differences in e(0)' do not result from the presence or absence of negatively charged surface residues or in differences in the types of hydrophobic residues found close to the [4fe-4 ...	2002	11704670
[four cases of non-clostridial gas gangrene with diabetes mellitus].	we report four cases of non-clostridial gas gangrene. all cases were associated with diabetes mellitus as the underlying disease. case 1: a 60-year-old male developed an ulcerative lesion on the dorsum of his left foot. peptostreptococcus asaccharolyticus, citrobacter freundii and staphyrococcus epidermidis were identified in culture from odoriferous pus. case 2: a 81-year-old female developed a lesion on her vulva spreading to the right lower abdomen. bacteroides bivius, peptostreptococcus asac ...	2002	11915237
the effects of religious backgrounds on emotional problems.		2002	126985
a new device to support handicapped patients during venography or myelography.	the authors describe a seating device which holds the patient in position when the x-ray table is tilted and permits non-weight-bearing venograms to be obtained in handicapped as well as normal patients. simultaneous bilateral venography reduces the study time considerably. the device is also helpful in lumbar myelography in paraplegics.	2003	149336
[70th birthday of dr. karel raska].		2003	161871
the relationship of the pulp and periodontium.		2003	149297
[combined treatment of rheumatological patients at a health resort].		2003	154775
[laparoscopy in the diagnosis of hepatobiliary anomalies].		2003	152516
antimicrobial properties of self-etching primer-bonding systems.	self-etching primers are now considered the new generation of dentin bonding systems that modify and incorporate the bacteria-containing smear layer into their bonding mechanism. the antibacterial effects of the self-etching primers clearfil se bond, mac bond, imperva fl bond, one-up bondf and prompt l-pop were evaluated using the bacteria streptococcus mutans atcc25175, peptostreptococcus anaerobius, peptostreptococcus prevotii, peptostreptococcus asaccharolyticus, lactobacillus acidophilus, la ...	2003	12670069
rapid identification of gram-positive anaerobic coccal species originally classified in the genus peptostreptococcus by multiplex pcr assays using genus- and species-specific primers.	here, a rapid and reliable two-step multiplex pcr assay for identifying 14 gram-positive anaerobic cocci (gpac) species originally classified in the genus peptostreptococcus (anaerococcus hydrogenalis, anaerococcus lactolyticus, anaerococcus octavius, anaerococcus prevotii, anaerococcus tetradius, anaerococcus vaginalis, finegoldia magna, micromonas micros, peptostreptococcus anaerobius, peptoniphilus asaccharolyticus, peptoniphilus harei, peptoniphilus indolicus, peptoniphilus ivorii and pepton ...	2003	12855723
[discitis in small children (author's transl)].	discitis was diagnosed in 5 children under 3 years of age, the initial clinical manifestations being difficulty in walking and abdominal pains in one case. diagnosis was not made before periods varying from 8 days to 3 months, and no etiological basis for the disease was discovered. pinching of the disc was always present in the first radiographic image, and the vertebral plate was ill-defined in 3 cases. repeat radiological examinations were carried out in 3 children after 6 months, 2 and 5 yea ...	2004	529226
newer knowledge of skeletogenesis: macromolecular transitions in the extracellular matrix.	morphogenesis of skeletal tissues is accompanied by dramatic changes in the types and amounts of extracellular macromolecules synthesized. these changes correlate with the morphological and structural characteristics of each tissue type involved in skeletogenesis, viz. mesenchyme, cartilage and bone. at the beginning of skeletogenesis, a hyaluronate-rich extracellular matrix may provide a beneficial milieu for mesenchymal cell migration and proliferation, and prevent precocious differentiation. ...	2004	343963
pharmacokinetics and pharmacodynamics of intravenous levofloxacin at 750 milligrams and various doses of metronidazole in healthy adult subjects.	the purpose of this investigation was to evaluate the steady-state pharmacokinetics, pharmacodynamics, and safety of intravenous levofloxacin at 750 mg administered once daily combined with three different dosages of intravenous metronidazole (500 mg every 8 h [q8h], 1,000 mg q24h, and 1,500 mg q24h). eighteen healthy adult subjects received all three combinations in a randomized, crossover fashion. serial blood and urine samples were collected on the third day of each study period. the 24-h are ...	2004	15561831
usefulness of long-acting nitrates in cardiovascular disease.		2004	415607
[how does one calculate the activity of a radiodiagnostic service?].		2004	529236
prognistic relevance of radiation induced immune suppression in breast carcinoma.	the extent of radiation induced immune suppression was analysed in 100 patients with carcinoma of the breast. the relative changes of lymphocyte counts and stimulations by pha and ppd were similar in patients who differed with regard to age, size of tumour and its malignancy grade or axillary node condition. moreover, no difference in the degree of radiation induced immune suppression existed between patients who developed recurrent disease and those who remained free of disease during a follow- ...	2004	525447
prophylactic infusion therapy in hemophilia.		2004	478502
[epidemiological studies on systemic lupus erythematosus(sle) (1)].		2005	1169520
[measured running in the overall treatment of chronic ischemic heart disease].		2005	538895
properties of the thermostable glutamate dehydrogenase of the mesophilic anaerobe peptostreptoccus asaccharolyticus purified by a novel method after over-expression in an escherichia coli host.	peptostreptococcus asaccharolyticus glutamate dehydrogenase (l-glutamate: nad+ oxidoreductase (deaminating); ec 1.4.1.2) overexpressed in escherichia coli has been purified by two new methods. enzyme made by the first method showed remarkable thermophilicity, with a temperature optimum of 60 degrees c, and also thermostability, which suggested the second, simpler method, incorporating a heat step. this produced 94 mg of homogeneous protein per litre culture medium. the basic kinetic parameters f ...	2005	15727821
[role of obligate anaerobes in infections in hospitalized patients and therapeutic options].	monitoring of prevalence and antibiotic susceptibility of strictly anaerobic bacteria, causing infections in hospitalized patients, constitutes a part of a program for prudent use of antibiotics. the aim of the study was to assess prevalence of strictly anaerobic bacteria in patients hospitalized in a tertiary care hospital in 2001-2002 with reference to empiric antibiotic therapy. the most common gram-positive bacteria were clostridium difficile--27.7%, peptostreptococcus spp. and peptoniphilus ...	2005	16134392