Publications

'baby doe'--a medical ethical issue.		1985	4024642
early postnatal development of eeg and sleep-waking cycle in two inbred mouse strains.	the postnatal maturation of brain electrical activity and sleep-waking cycle were studied in two inbred mouse strains (c57bl and balbc), previously differentiated in their sleep patterns at adult age. genetic differences are evident during the first postnatal period (until day 12) in the maturation of electrical activity which is both earlier and slower in c57bl than in balbc. on the other hand, from day 12 onwards, as soon as the sleep-waking cycle can be defined by using eeg morphology to sele ...	1985	4041918
determination of urinary lysozyme during aminoglycoside therapy--appraisal of lysozyme inhibition caused by gentamicin and tobramycin.		1985	4075521
biochemical and molecular epidemiology of human cancer risk.		1985	3903477
the interaction of tumour-localizing porphyrins with collagen, elastin, gelatin, fibrin and fibrinogen.	we have already reported in balb c mouse transplantable mammary carcinoma, that uroporphyrin i and iii are superior as tumour localizers when compared to hematoporphyrin derivative and a derivative thereof, photofrin ii. this study compares the binding of porphyrins to proteins which may be found in tumour cells or stroma to investigate whether there is a common binding determinant. coproporphyrin iii and deuteroporphyrin ix which are non-tumour localizing porphyrins, were also part of the compa ...	1985	3836016
osteocalcin in human serum: a circadian rhythm.	osteocalcin, the vitamin k-dependent protein synthesized in bone, is found in blood. the level of circulating osteocalcin has recently been used as an indicator of the rate of bone turnover. we measured serum osteocalcin during 24-h periods in 6 normal 20- to 30-yr-old men and 4 women. blood was sampled via an indwelling venous catheter every 30 or 60 min for 24 h. circadian rhythmicity in circulating osteocalcin was found in 9 of the 10 individuals studied. osteocalcin levels fell during the mo ...	1985	3871789
presence of soluble, mycobacterium leprae-derived antigen in the inflammatory exudate of reactional lepromatous leprosy.		1985	3903409
the ability of single sex infections of schistosoma japonicum to induce resistance to reinfection in mice.	in four experiments, mice harbouring an average 50, 76 or over 200 schistosoma japonicum female worms were not resistant when challenged six to eight weeks after infection. the female worms from these single sex infections were stunted and immature (average length 4.6 mm) and induced no overt pathology in the host. male worm burdens of 60, 135 or 140 also induced little or no resistance to challenge in the host. the males from these single sex infections were fully grown for their age (average l ...	1986	3091678
low molecular weight eosinophil chemotactic factor (ecf) in the serum of murine schistosomiasis japonica.	eosinophil chemotactic activity was detected in the serum obtained at an acute stage of murine schistosomiasis japonica. gel filtration of the dialyzable fraction of the serum on sephadex g25 showed that the chemotactic component had an apparent molecular weight of less than 1,000. it was stable to heating, but was sensitive to pronase or carboxypeptidase a digestions, indicating its peptide nature. eosinophil chemotactic activity of the dialysate of the serum from mast cell-deficient w/wv mice ...	1986	3080375
high oxygen requirements of dyskinetic cardiac muscle.	this study assesses the regional oxygen requirements of muscle segments that are beating and working, beating and empty, arrested and decompressed, and nonischemic that move dyskinetically. regional oxygen demands were evaluated by producing a dyskinetic segment by infusing regional cardioplegic solution through a left anterior descending coronary artery catheter with and without extracorporeal circulation. the results show that the o2 demands of the perfused dyskinetic cardiac muscle segment (4 ...	1986	3747582
the effect of altered hormonal states on some enzymes of gluconeogenesis in the genital organs of immature female rhesus monkeys.		1986	3779756
telomeric fusion in pre-t-cell acute lymphoblastic leukemia.	telomeric fusion, a rare phenomenon, was observed in malignant cells from the peripheral blood of an 18-year-old male with rapidly progressive pre-t-cell acute lymphoblastic leukemia (all). only two comparable cases, both with b-cell all, have been reported with telomeric fusion in neoplasia. all of the leukemic cells examined from our patient had two chromosome abnormalities consisting of partial triplication (trp) of chromosome 2 and a derivative chromosome 3. approximately a third of the leuk ...	1986	3488255
3h-mepyramine binding and histamine-stimulated camp accumulation in mammalian retina.	the specific binding of different amounts of 3h-mepyramine to the bovine retina revealed a quasi-hyperbolic curve which approached saturation at 3h-ligand concentration over 9-12 nm. scatchard analysis of the binding data showed two binding sites with kd values of 0.76 nm and 7.3 nm and bmax of 49.3 and 194.6 fmole/mg protein, respectively. in the guinea-pig brain 3h-mepyramine bound to a single population of binding sites with kd value of 1.6 nm and bmax of 291 fmole/mg protein. various h1-anti ...	1986	3014843
treatment of idiopathic west and lennox-gastaut syndromes by intravenous administration of human polyvalent immunoglobulins.	a total of 7 patients (3-21 years old) suffering from an intractable "primary" lennox-gastaut syndrome (lgs) were treated with i.v. high doses of polyvalent human immunoglobulins. of these patients 6 improved following such treatment with a decrease in fits and an improvement in the eeg. hypotheses about the contribution of the treatment and immunopathological factors in some cases of idiopathic lgs are discussed.	1986	3792407
localization of the human tyrosine hydroxylase gene to 11p15: gene duplication and evolution of metabolic pathways.	phenylalanine hydroxylase (pah) and tyrosine hydroxylase (th) are consecutive enzymes in the metabolic pathway leading to the production of catecholamine neurotransmitters. a comparison of recently available sequence data of these enzymes in the rat indicates about 70% homology in the 3' coding regions. we have localized th by in situ hybridization to human chromosome region 11p15. consideration of this assignment and that of pah to chromosome 12, together with the known distribution of other pa ...	1986	2872999
case records of the massachusetts general hospital. weekly clinicopathological exercises. case 29-1986. an asplenic woman with evidence of sepsis and diffuse intravascular coagulation after a dog bite.		1986	3724816
[a case of leiomyosarcoma of the kidney].	leiomyosarcoma of the left kidney seen in a 58-year-old man is reported. on april 10, 1982, he complained of left flank pain. he visited our hospital and left solitary renal cyst was suspected. he had been treated as an outpatient, but left flank pain became exacervated. on may 18, he was admitted to our hospital. on june 7, radical nephrectomy was done under the diagnosis of left renal cell carcinoma. at operation, the tumor invased directory to the psoas muscle and abdominal wall, and could no ...	1986	3728230
virulence mechanisms associated with clinical isolates of non-o1 vibrio cholerae.	twenty one isolates of non-o1 v. cholerae from patients with diarrheal illness were examined for the presence of potential virulence mechanisms. the motile strains (90%) produced cell-associated mannose-sensitive hemagglutinins which reacted with human group o, chicken, sheep and rabbit erythrocytes. motile isolates also attached to embryonic intestinal epithelial cells (atcc 407), and the adherence was not inhibited by the presence of 1% d-mannose. all vibrio isolates hemolyzed sheep erythrocyt ...	1986	3739461
[seasonal changes in the count of mites of the family pyroglyphidae in houses in the city of cheboksary].	the results of human dwelling houses surveying for the presence of home dust mites and study of seasonal changes of their quantity in the town of cheboksary are given. it has been found that the old wooden houses are inhabited by mites, in modern blocks of flats they are met rather seldom. the quantity of alive mites is maximum in september-october. the common number of mites is the greatest in december-january although the quantity of alive mites at this time is only 6.8%.	1986	2939887
replacement of 5-methylcytosine by cytosine: a possible mechanism for transient dna demethylation during differentiation.	in an earlier study it was discovered that when friend erythroleukemia cells (felc) were exposed to a variety of chemical agents capable of inducing differentiation, their dna underwent genome-wide transient demethylation. in an attempt to elucidate the biochemical mechanism responsible for this phenomenon we have induced felc with 5 mm hexamethylenebisacetamide and labeled the dna in vivo with a density label, 5-bromodeoxyuridine, and a radioactive label, deoxy[5-3h]cytidine. newly replicated d ...	1986	3458243
t(4;11) translocation-associated acute leukemia: an update.		1986	3465430
analysis of the internal nuclear matrix. oligomers of a 38 kd nucleolar polypeptide stabilized by disulfide bonds.	when rat liver nuclei are treated with the sulfhydryl cross-linking reagent sodium tetrathionate (natt) prior to nuclease treatment and extraction with 1.6 m nacl, residual nucleoli and an extensive non-chromatin intranuclear network remain associated with the nuclear envelope. subsequent treatment of this structure with 1 m nacl containing 20 mm dithiothreitol (dtt) solubilizes the intranuclear material, while the nuclear envelope remains structurally intact. we have isolated and partially char ...	1986	3514251
cell cycle dynamics of maturation-promoting factor during mouse oocyte maturation.	the changes in activity of a cytoplasmic maturation-promoting factor (mpf), capable of inducing resumption of meiosis when injected into starfish oocytes, were examined during mouse oocyte maturation. mpf appeared first at the time of germinal vesicle breakdown (gvbd), and its activity fluctuated along with the meiotic cycle: it reached a peak at the first metaphase and disappeared at the first polar body emission. then mpf reappeared and reached a peak again at the second metaphase. thus, a goo ...	1986	2832978
the epidemiology of the acquired immunodeficiency syndrome.		1986	3008169
cardiovascular hypertrophy in hypertension. arthur c. corcoran memorial lecture.		1986	3013769
cardiovascular hypertrophy in hypertension. arthur c. corcoran memorial lecture.		1986	3013769
structurally related bacillus thuringiensis delta-endotoxins display major differences in insecticidal activity in vivo and in vitro.	many strains within the 22 serotypes of bacillus thuringiensis produce crystal delta-endotoxins with slight differences in their insecticidal toxicity spectrum in vivo. since the basis of this specificity is unknown, we chose to compare the activity of delta-endotoxins from three strains: b. thuringiensis var. kurstaki hd-1, var. aizawai hd-249 and var. thuringiensis hd-350, both in vivo and on insect cell lines in vitro. immunoblotting with antisera to activated var. kurstaki p1 lepidopteran to ...	1986	3027111
antibodies to distal carboxyl terminal epitopes in the v-fms-coded glycoprotein do not cross-react with the c-fms gene product.	the product of the v-fms oncogene is an integral transmembrane glycoprotein that is closely related to the cell surface receptor for the macrophage colony stimulating factor, csf-1. a fragment of the v-fms gene encoding a major portion of the extracellular amino terminal domain, the membrane-spanning segment, and the entire carboxyl terminal tyrosine kinase domain of the glycoprotein was molecularly cloned into an inducible prokaryotic expression plasmid. polypeptide products consisting only of ...	1986	2425481
effect of synexin on aggregation and fusion of chromaffin granule ghosts at ph 6.	fusion of chromaffin granule ghosts was induced by synexin at ph 6, 37 degrees c, in the presence of 10(-7) m ca2+. to study the kinetics and extent of this fusion process we employed two assays that monitored continuously mixing of aqueous contents or membrane mixing by fluorescence intensity increases. in both assays chromaffin granule ghosts were either labeled on the membrane or in the included aqueous phase. the ratios of blank to labeled chromaffin granule ghosts were varied from 1 to 10. ...	1987	2443172
intracellular ca2+ and protein kinase c modulate k+ current in guinea pig heart cells.	effects of protein kinase c (pkc) and intracellular calcium ion (cai2+) on the delayed rectifier k+ current (ik) were investigated in the single ventricular cells of guinea pig by use of an internal-dialysis method and a whole cell voltage-clamp technique. 12-o-tetradecanoylphorbol-13-acetate (tpa, 10(-9) m), an activator of pkc, increased the amplitude of ik in the presence of cai2+ higher than 10(-10) m. this effect of tpa was mimicked by a synthetic diacylglycerol, 1-oleoyl-2-acetylglycerol ( ...	1987	2446513
the leeuwenhoek lecture, 1986. environmental carcinogens and papillomaviruses in the pathogenesis of cancer.	in many areas of the world there is a geographically localized high incidence of alimentary and bladder cancer in cattle. studies in western scotland have demonstrated that this phenomenon is associated with ingestion of bracken fern. however, the affected animals and herds were shown also to have an unusually high infection rate of alimentary papillomas caused by a previously unrecognised bovine papillomavirus (bpv) and that these tumors could undergo malignant transformation. long-term field a ...	1987	2888116
onset of steroidogenesis and differentiation of functional lh receptors in rat fetal testicular cultures.	the present study was performed to investigate in vitro the onset of steroidogenesis and the responsiveness to lh in rat fetal testes. the male gonads explanted on days 12.5, 13.5, and 14.5 of gestation in m199 produced testosterone from 15.5 days as is the case in vivo dcamp (1 mm) induced an anticipated steroidogenesis on day 14.5 with secretions of testosterone (0.026 +/- 0.003 ng/gonad/24 h) and progesterone (0.078 +/- 0.005 ng/gonad/24 h), whereas lh (100 ng/ml) has no effect. antiandrogens ...	1987	2823733
involvement of the membrane skeleton in the regulation of the camp-independent protein kinase and a protein phosphatase that control protein synthesis.		1987	2832271
[social medicine aspects of increasing longevity and aging].	in all highly industrialized nations the survival curve shows a tendency towards "rectangulation", i.e. forming a plateau with a following decline in the elder ages. the life span remains unchanged as a specificity of the human genus. morbidity in old age shows the same tendency to rectangulation. the majority of old people today are far more vital and healthy than in the past. increasing longevity, persisting vitality in old age, in connection with acceleration and postponement of the menopause ...	1987	3630320
tumor necrosis factor is partly responsible for the differentiation of mouse myeloid leukemia cells.	when mouse myeloid leukemia m1 cells were incubated with 0.1% tns(serum containing tumor necrosis factor, tnf), they were induced to differentiate into macrophage-like cells within 14 h. the stability of the differentiation activity to heat treatment was very similar to that of the cytotoxicity of tnf for l929 cells. the differentiation activity could not be separated from the tnf activity by sequential chromatography procedures using deae-sephadex and sephacryl s-200 or by isoelectric focusing. ...	1987	3440028
the biochemical basis of niridazole toxicity.		1987	3509430
comparative biodistributions and rates of catabolism of radiolabelled anti-cea monoclonal antibody and control immunoglobulin in nude mice with human tumour xenografts showing specific antibody localization.	the rate of catabolism of a radiolabelled monoclonal anti-cea antibody has been compared with that of control igg1 in control nude mice and in mice with cea producing xenografts and antigen negative xenografts. the rate of catabolism of antibody, but not of igg, was increased 3-4 fold in mice with xenografts localizing the antibody, but not in mice with antigen negative tumours. there was no evidence of immune complex formation and/or clearance of antibody from the serum of xenografted mice and ...	1987	3478194
measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study.	a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ...	1987	3315296
measurement of regional myocardial perfusion and mass by subselective hydrogen infusion and washout techniques: a validation study.	a technique was developed for measuring regional myocardial perfusion by intracoronary infusions of hydrogen (h2)-saturated saline. h2 concentration was detected during washout in the pulmonary artery by means of the voltage response of a platinum-tipped electrode. regional myocardial perfusion was calculated from the h2 exponential desaturation curve according to the kety-schmidt principle. in 16 anesthetized open-chest dogs, validation of this technique was performed at baseline, reduced (sten ...	1987	3315296
anti-candida drugs--the biochemical basis for their activity.	the past years have seen a continuous effort toward the synthesis of new antifungal agents. most of them belong to the n-substituted imidazoles and triazoles. another interesting series of antifungals are the allylamines. biochemically, both the azole derivatives and the allylamines belong to the class of ergosterol biosynthesis inhibitors and thus differ from the polyene macrolide antibiotics. indeed, it is now believed that the antifungal action of the polyenes, nystatin and amphotericin b, is ...	1987	3319421
a comparison of the effects of streptozotocin, n-methylnitrosourea and alloxan on isolated islets of langerhans.	isolated normal rat islets were pre-incubated with streptozotocin (stz), n-methylnitrosourea (mnu) or alloxan for 5, 10, 30 or 60 minutes at 0 degree c or 37 degrees c, and then were washed and incubated at 37 degrees c for 60 minutes with glucose (16.7 mm). suppression of the insulinotropic response to glucose during incubation required 10 minutes of pre-incubation with the nitrosoureas whose effects were directly related to concentration and were temperature dependent. the suppressive effects ...	1987	2954869
identification of mortierella wolfii, a causative agent of mycotic abortion in cattle.		1987	3668761
characterization of the na+/h+ exchanger during maturation of hl-60 cells induced by dimethyl sulfoxide.	we have studied the activity of the na+/h+ exchanger during dimethyl sulfoxide (me2so)-induced maturation of the human promyelocytic leukemia cell line hl-60. 22na uptake was measured in cells preloaded with li+ or nh+4 in order to specifically activate the na+/h+ exchanger. measurement of the rate of uptake as a function of sodium concentration revealed a decrease in km for na+ from 38 +/- 3 to 13 +/- 1 mm after 20-24-h treatment with me2so. vmax was not changed significantly. inhibition of the ...	1987	3036846
congenital and acquired neuromuscular disease of young dogs and cats.	neuromuscular disorders in small animals include a diverse group of congenital and acquired diseases. the prognosis will vary according to the disorder and the portion of the motor unit affected. a number of diseases might be satisfactorily treated (for example, myasthenia gravis, congenital myotonia), whereas others may be self-limiting (for example, hereditary myopathy of labrador retrievers). accurate diagnosis is necessary for establishing a prognosis and treatment plan suitable to the patie ...	1987	3300000
effects of endogenous pyrogen and prostaglandin e2 on hypothalamic neurons in rat brain slices.	we investigated the effects of endogenous pyrogen and prostaglandin e2 (pge2) on the preoptic and anterior hypothalamic (poah) neurons using brain slice preparations from the rat. partially purified endogenous pyrogen did not change the activities of most of the neurons in the poah region when applied locally through a micropipette attached to the recording electrode in proximity to the neurons. this indicates that partially purified endogenous pyrogen does not act directly on the neuronal activ ...	1987	3497702
topical minoxidil in early androgenetic alopecia.	a randomized, double-blind trial was conducted to determine the relative efficacy and safety of topical minoxidil solution for progressive male pattern baldness. one hundred fifty men with a discernible vertex balding spot were randomly assigned to treatment with either 2% topical minoxidil, 3% topical minoxidil, or placebo. after 4 months, patients receiving placebo were crossed over to 3% minoxidil. the study was concluded after 12 months of treatment, at which time 100 subjects were evaluable ...	1987	3549807
intracellular water and ionic shifts during growth and ageing of rats.	as measured by x-ray microanalysis of frozen-dried cryosections, physiological concentrations of phosphorus, na+, k+ and mg2+ show a biphasic age dependence in some compartments of rat hepatocytes, especially in the regions of condensed chromatin associated with the actively transcribed genes. this could be consistent with a reactivation of the protein synthesizing apparatus in old livers. the decrease in intracellular water concentrations in hepatocytes and myocytes during ageing is shown to be ...	1987	3600050
rate responsive pacing compromise: response time vs. heart rat variation.		1987	3607189
electrochemical immunoassay combined with column liquid chromatography: determination of phenytoin in human serum.	a new electrochemical immunoassay combined with column liquid chromatography has been developed for the determination of phenytoin in human serum. phenytoin was labelled with the electrochemically active nitroxide, and the separation of the free labelled antigen from other electrochemically active compounds in serum was accomplished by the use of gel chromatography. serum samples were mixed with the antibody and the labelled antigen, incubated for 90 min, and then a 100-microliter aliquot of the ...	1987	3624390
functional defect of zinc transport in patients with crohn's disease.	in the present study, we investigated the question whether the zinc deficiency in crohn's disease is, at least partly, related to defective zinc entry at the jejunal brush border membrane. brush border membrane vesicles (bbmv) were prepared from surgically resected pieces of morphologically intact human jejunum by a mg++ precipitation method. we studied zinc, d-glucose, sodium and d-mannitol uptake into bbmv in 27 patients: 1) 20 patients with crohn's disease 2) 7 controls. in 8/20 patients with ...	1987	3666668
reduction and reoxidation of the neurotoxin ii from the scorpion androctonus australis hector.	reoxidation of the totally reduced scorpion neurotoxin ii from androctonus australis hector (four disulfide bridges) has been investigated. the totally reduced toxin was highly insoluble in neutral and alkaline conditions, which prevented the use of the usual air oxidation process for renaturation. we tested a new method in which the reduced molecules were first solubilized in 10% (v/v) acetic acid and then oxidized by air through dialysis against a series of buffers with a slow ph gradient from ...	1987	3667073
methylergometrine antagonizes 5 ht in the temporal artery.	methylergometrine, the main metabolite of methysergide, has 40-times greater 5 ht antagonistic potency than methysergide in isolated segments of human temporal artery. it is suggested that methylergometrine is the "active" drug when methysergide is used in migraine prophylaxis, and that the two compounds should be compared in prophylactic trials in migraine.	1987	3691600
modulation of histone h3 variant synthesis during the myoblast-myotube transition of chicken myogenesis.	we have previously reported that nucleosomal histones are synthesized by cultured, postmitotic myotube cells at 9-29% of the rate in their dividing myoblast precursors (a. m. wunsch, a. l. haas, and j. lough, 1987, dev. biol. 119, 85-93). in that study, histones were separated by two-dimensional polyacrylamide gels containing 8 m urea in the first-dimension to optimally separate variants of the h2a class. to separate and compare synthesis of variants in the h2b and h3 classes during myogenesis, ...	1987	3792638
sympathoadrenal mechanisms in cardiovascular responses to naloxone after hemorrhage.	five rabbits were allotted to each of six treatments on a matched-individual basis. treatments were none, sham, total adrenalectomy with adrenocorticoid replacement, intravenous guanethidine (15 mg x kg-1 x day-1), adrenalectomy + guanethidine, and adrenal medullectomy. the conscious rabbits were bled 20 ml/kg over 5 min. naloxone (6 mg/kg) was injected intravenously. the responses of arterial pressure and of plasma epinephrine (e) and norepinephrine (ne) concentrations were measured. factorial ...	1987	3812714
coronary hemodynamics and myocardial metabolism of lactate, free fatty acids, glucose, and ketones in patients with septic shock.	to investigate disturbances in the coronary circulation and myocardial metabolism during septic shock, we examined coronary sinus blood flow and myocardial substrate extraction in 40 patients with septic shock and 13 control patients. patients with coronary artery disease were excluded from this study. the global hemodynamic pattern of the septic patients was characterized by a lower stroke volume, despite an elevated cardiac index. coronary sinus blood flow was high (187 +/- 47 vs 130 +/- 21 ml ...	1987	3815765
production and characterization of rat monoclonal antibodies with an apparent specificity for certain lung cancers and their potential clinical application.	this paper describes two rat monoclonal antibodies (mabs) (1a6 and 2h7, both igg2a) directed against a human adenocarcinoma of the lung (a549). enzyme-linked immunosorbent assay (elisa) and immunocytochemistry (avidin-biotin-complex method) as the screening of the hybridoma cells revealed that 1a6 and 2h7 mab mainly reacted with adenocarcinoma of the lung, breast and pancreas, and 2h7 mab also recognized large cell carcinoma, epithelia of the lung, trachea, tongue or small cell carcinoma of the ...	1987	3131562
ferritin synthesis in differentiating friend erythroleukemic cells.	we have investigated the regulation of ferritin synthesis during induction of friend erythroleukemic cells by dimethyl sulfoxide. northern blot analysis shows that mouse ferritin h and l mrnas each contain approximately 1.1 kilobases. the levels of both mrnas increase after addition of dimethyl sulfoxide in a biphasic manner. after a sharp rise in the first 6 h, the levels decline and then rise again over the next 90 h. these increases in mrna levels reflect increased transcription of both mrnas ...	1987	3475274
effect of clomiphene isomers on progestin synthesis in cultured human granulosa cells.	the effect of en-clomiphene and zu-clomiphene (10(-9)-10(-5) m) on progestin synthesis in cultured human granulosa cells was studied under basal conditions and in the presence of lh (100 ng/ml). granulosa cells were obtained from either pre-ovulatory follicles of clomiphene-hmg-stimulated cycles or from large follicles of mid-to-late follicular phase of spontaneous cycles. the basal and lh-stimulated progesterone accumulation was dose-dependently reduced by en- and zu-clomiphene (10(-6)-10(-5) m ...	1987	3117835
conversion of linoleic acid and arachidonic acid by skin epidermal lipoxygenases.	two different lipoxygenases have been identified in human and rat epidermis. one lipoxygenase has a (n-9)-specificity, converts arachidonic acid into 12-hydroxyeicosatetraenoic acid (12-hete), and has been described by several investigators. linoleic acid is not a substrate for this enzyme. the other lipoxygenase, with (n-6)-specificity, converts arachidonic acid into 15-hete and linoleic acid into 13-hydroxyoctadecadienoic acid (13-hod). especially the latter lipoxygenase is thought to be invol ...	1987	3113487
[innervation of the human hand (based on data from clinical observation].		1988	3169415
human alveolar macrophage and blood monocyte inhibition of fibroblast proliferation. evidence for synergy between interleukin-1 and tumor necrosis factor.	stimulated human blood monocytes and alveolar macrophages are known to elaborate a soluble factor(s) that inhibits fibroblast proliferation by stimulating fibroblast prostaglandin (pg) production. however, the factor(s) mediating these effects has never been completely characterized and its relationship to known cytokines has never been fully defined. in this study, we demonstrate that this factor(s) is partially trypsin-sensitive, is between 16 and 24 kilodaltons molecular weight, and elutes be ...	1988	3264485
growth of pure cultures of retinal pigment epithelial cells using chorioretinal biopsies from the pig.		1988	3289957
[quantitative estimation of synaptic acetylcholinesterase inhibition with galanthamine using parameters of miniature endplate currents].	miniature end-plate currents (mepc) were recorded in voltage clamped muscle fibers of the rat diaphragm at different degrees of acetylcholinesterase (ache) inhibition with galanthamine. a model has been suggested connecting the increase in mepc amplitude with the concentration of a competitive reversible ache inhibitor. using the model suggested, the changes in the junctional ache activity inhibited with different concentrations of galanthamine were estimated. the calculated value of the inhibit ...	1988	3390582
antibody-dependent cell-mediated growth inhibition of rat thyroid cells, frtl-5, in patients with autoimmune thyroid diseases.	we studied antibody-dependent mononuclear cell-mediated growth inhibition of thyroid cells in 18 untreated patients with graves' disease, 18 patients with chronic thyroiditis, and 15 normal subjects by measuring the ability of their sera to inhibit [3h]thymidine incorporation into dna in a rat thyroid cell line, frtl-5, in the presence of normal mononuclear cells. [3h]thymidine incorporation was significantly inhibited in the presence of sera from patients with graves' disease and chronic thyroi ...	1988	3400407
delayed response deficit in monkeys by locally disturbed prefrontal neuronal activity by bicuculline.	effects of local injection of a gamma-aminobutyric acid antagonist, bicuculline (10-30 micrograms dissolved in saline) in the principal prefrontal cortex on the delayed response task were investigated in two monkeys. on a visual go signal, the monkeys rotated a handle to the left or to the right (go period) according to a visual cue (left or right; 1 s) presented 4 s earlier. bicuculline induced bursting activity at the injected site 1-2 min after the injection and 5 min later the burst activity ...	1988	2849457
naloxone attenuation of sham feeding is modified by manipulation of sucrose concentration.	the time course of sucrose (5, 10 and 20%; w/v) sham feeding was monitored in one hour tests. intake levels increased as a function of concentration. naloxone (1.25 mg/kg, ip) attenuated the sham feeding of 10% sucrose solution in gastric fistulated rats, without affecting initial intake rates. furthermore, after naloxone the intake pattern of 10% sucrose was identical to that for 5% sucrose in untreated rats. in a second test, substitution of 10% sucrose by a 20% solution after 15 min of sham f ...	1988	2853384
smoking-related alterations in epidermal growth factor and insulin receptors in human placenta.	studies characterized insulin and egf receptors in human placental tissue from smokers and nonsmokers. specific binding of 125i-labeled insulin and egf to placental membranes was not different for nonsmokers compared with smokers. egf and insulin receptor kinases were further studied using a wheat germ agglutinin-purified preparation of solubilized placental membrane proteins. in extracts from the nonsmoker group, egf stimulated the active phosphorylation of mr 170,000 and 140,000 protein bands, ...	1988	2843746
isolation of a variant endogenous avian leukosis virus: non-productive exogenous infection with endogenous viruses containing p27 and p27(0).	ten replication-competent endogenous avian leukosis viruses (alvs) with subgroup e specificity were isolated from six commercial chicken lines of mixed breed. all the alvs isolated belonged to a variant class of endogenous viruses with a major group-specific antigen, p27, identical in size to that of exogenous viruses and different from the p27 degrees of endogenous viruses isolated from white leghorn chickens. it was possible to infect chickens exogenously with endogenous viruses (containing p2 ...	1988	2832527
altered t-cell helper/suppressor ratio in mice chronically exposed to amyl nitrite.		1988	2902515
homology of glucosyltransferase gene and protein sequences from streptococcus sobrinus and streptococcus mutans.	the sequences of glucosyltransferase genes from streptococcus sobrinus (gtfi) and streptococcus mutans (gftb) were compared and show a high degree of homology. there is a 57.7% homology of nucleotides in the genes and a 56.7% homology of amino acids in the deduced protein sequences. the g + c content for the protein-coding region is 43.6% for s. sobrinus and 41.2% for s. mutans. internal repeating sequences present in both proteins exhibit some difference in sequence pattern.	1988	2971696
diabetic embryopathy and fuel-mediated organ teratogenesis: lessons from animal models.	the growing recognition that faulty maternal metabolism during early organogenesis may be implicated in the increased incidence of birth defects in pregnancies complicated by diabetes has prompted worldwide efforts to institute improved preconceptional metabolic regulation. however, the failure to identify the periods of greatest risk for diabetic embryopathy, the mediating teratogen(s), and the underlying mechanisms have complicated attempts to establish precise therapeutic guidelines and targe ...	1988	3053387
interaction of the bacillus subtilis phage phi 105 repressor dna: a genetic analysis.	the bacillus subtilis phage phi 105 repressor specifically recognizes a 14-bp operator sequence which does not exhibit 2-fold rotational symmetry. to facilitate a genetic analysis of this sequence-dependent dna binding a b. subtilis strain was constructed in which mutations affecting the phi 105 repressor-operator interaction cause a selectable phenotype, chloramphenicol resistance. after in vivo mutagenesis, we isolated and mapped 22 different mutations in the repressor coding sequence, 15 of w ...	1988	3135184
the effect of dietary cholesterol and fat on the risk of lung cancer in hawaii.	a population-based case-control study of the association between dietary lipids and lung cancer was carried out in hawaii between march 1983 and october 1985. the sample included 226 men and 100 women with lung cancer and 597 male and 268 female controls, frequency-matched to the cases on sex and five-year age group. personal interviews were conducted in the home by trained interviewers using a quantitative diet history method. the items in the questionnaire were chosen to provide an estimate of ...	1988	3195565
evaluation of the anderson baird-parker direct plating method for enumerating escherichia coli in water.	the direct plating (dp) method for enumerating escherichia coli in food was adapted for water analysis by membrane filtration and a standardized protocol was described. the dp method was found to give equal or better recoveries of e. coli than a membrane filtration method using 0.1% sodium lauryl sulphate agar; the repeatability of the dp method was markedly better. the necessity to transfer membranes from the non-selective medium tryptone soy agar (tsa) to the selective medium tryptone bile aga ...	1988	3280542
[coagulation and fibrinolysis associated with contact phase in plasma with high molecular weight kininogen deficiency].	congenital deficiency of high molecular weight kininogen (hmwk), first reported by saito et al. in 1975, is a rare disorder with homozygous defect of hmwk and only 14 cases have been reported so far. prolonged partial thromboplastin time and depressed intrinsic fibrinolytic activity in plasma interacted with negatively charged surface were characterized in these patients without any bleeding problem. on the other hand, patients associated with hemorrhagic disorder, such as haemophiliacs, showed ...	1989	2593236
toxicological evaluation of the cardiotonic isomazole in the dog.	acute, subchronic, and chronic toxicity studies were conducted in dogs with the new vasodilator/cardiotonic drug isomazole (imz) to support, in part, clinical investigations of this agent in humans. single oral doses of imz of 25, 50, or 100 mg/kg given to english pointer dogs (2/dose) caused a marked drop in systemic blood pressure and reflex-induced increases in heart rate to values well over 200 beats per minute. these responses were maintained for 12 to 22 hr depending on the dose given. one ...	1989	2612775
[pulmonary changes in aids].	we reviewed retrospectively the clinical records of 28 patients with aids staged group iv according to cdc-criteria. among these, 19 had pulmonary disease: most of them (n = 17) had pneumocystis carinii pneumonia (pcp). 12/17 patients with proven pcp displayed typical x-ray findings with diffuse perihilar interstitial infiltration sparing lung periphery. 3/17 had atypical features and 2 normal chest x-ray findings. these data are important to identify patients with pulmonary complications of aid ...	1989	2538872
[fibrinolytic and fibrin-stabilizing properties of human tissues].	fibrinolytic and fibrinase properties of the most of human organs and tissues have been studied. it has been established that fibrinolytic activity of the tissues highly varied. fibrinolytic agents of the tissues are rather unstable to dilution, in contrast to thromboplastin, that is stable to dilutions as 1:10000 and 1:100000 and higher. all the tissues contain both activators and inhibitors of fibrinolysis. in some of them inhibitors prevail, that proves their antifibrinolytic properties. tiss ...	1989	2806865
cell distribution in tracheal surface epithelium and the effects of long-term pilocarpine and atropine administration.	cell distribution and the effects of 12 daily injections of 80 mg/kg pilocarpine or 5 mg/kg atropine were studied in rat tracheal epithelium. ciliated, periodic-acid-schiff-positive (pas+), alcian blue-positive (ab+), nonstaining, and basal cells were counted and their order of occurrence was recorded. pilocarpine caused a decrease in ciliated and an increase in pas+, basal, and nonstaining cell numbers. atropine caused similar changes, although to a much lesser extent. ab+ cells were rare. cell ...	1989	2817427
surveillance of salmonellae in india for drug resistance.	out of 3001 salmonella received during 1984-85, 2985 strains were tested for antibiotic resistance and r-pattern transfer. multidrug resistance was observed in 73% of diarrhoeal and 43% of non-diarrhoeal isolates. antibiotic resistance markers were transferable in 46.6% of diarrhoeal and 41.1% of non-diarrhoeal isolates. two out of 4 salmonella sero-types isolated for the first time in india during this period were multidrug resistant.	1989	2687164
donor and recipient genotype and heterosis effects on survival and prenatal growth of transferred mouse embryos.	reciprocal embryo transfers amongst two inbred strains (c3heb/fej and swr/j) and their f1 cross (c3swf1) were used to examine donor and recipient genotype and heterosis effects on survival and prenatal growth of mouse embryos. among inbred strains, significant recipient genotype effects were detected for both embryo survival (p less than 0.01) and prenatal growth (p less than 0.05), while no donor genotype effects were observed. the recipient effect on overall embryo survival was due to a higher ...	1989	2760878
bile acid profile in a dog with cholangiocarcinoma.		1989	2536512
chromosomal mapping and gene disruption of the adhiii gene in aspergillus nidulans.	there are at least three alcohol dehydrogenases in aspergillus nidulans. adhiii has no obvious physiological function. we describe here the cloning of the adhiii gene (alcc), its mapping on linkage group vii by "reverse genetics", and the properties of multicopy transformants tested for their ability to grow on a range of alcohols (butan-1-ol being the best substrate tested for growth). we were unable to detect any obvious alteration in phenotype of a strain carrying a disrupted copy of the adhi ...	1989	2663191
cryptococcus neoformans var. neoformans and munia birds.	cr. neoformans var. neoformans has been persistently demonstrated in the saprobic environment of caged munia birds at a local zoological garden. the pathogen was also cultured from the beaks, feathers and legs of these birds. it is suggested that the role of munia birds should be further investigated to understand the ecology of this important human pathogen.	1989	2668756
[clinical and histopathological studies of the suture made of dcff-treated natural collagen fiber].	natural and synthetic fiber sutures used clinically, especially twist or braid type ones, will remain to the region after healing by the first intention and often cause inflammation by capillarity. it is necessary to remove those sutures from healing wounds. to obviate this necessity, we separated collagen fibers from a tendon of a rat's tail, gave them dcff treatment for immunological tolerance, and made collagen sutures on an experimental basis. the discussion on the clinical, histopathologica ...	1989	2668831
[synthetic cephalosporins. v. synthesis and antibacterial activity of 3-alkylthio-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(o-substituted oxyimino)acetamido]cephalosporins and related compounds].	3-alkylthio-7 beta-[(z)-2-(2-aminothiazol-4-yl)-2-(o-substituted oxyimino)acetamido]cephalosporins (6 and 7) and the 3-methoxy analogues (10) were prepared by coupling diphenylmethyl 7-amino-3-alkylthio-3-cephem-4-carboxylate (1 and 2) or diphenylmethyl 7-amino-3-methoxy-3-cephem-4-carboxylate with (z)-2-(2-tritylaminothiazol-4-yl)-2-(o-substituted oxyimino)acetic acid (4), followed by deprotection and subjected to examination of antibacterial activities. the pivaloyloxymethyl esters (8 and 9) o ...	1989	2698417
current management of salivary gland tumors. part 2.	the authors base their treatment of salivary gland malignancies on the size of the primary and the histopathologic diagnosis. group 1 includes smaller tumors in the t1 and t2 classification with cell types that are associated with slow growth. a parotidectomy is usually sufficient therapy for tumors in this group. group 2 contains t1 and 2 tumors with more aggressive behavior. total parotidectomy is indicated here, with postoperative radiotherapy. t3 tumors and patients with nodal metastasis or ...	1989	2701414
a preliminary survey of parasitic infections and nutritional status among school children in riyadh, saudi arabia.	a total of 320 school children in the model institute of the capital in riyadh, were clinically and parasitologically examined. thirty-five (10.94%) had intestinal parasites. entamoeba coli (61.91%) was found more frequently among the commensals, while giardia lamblia (28.57%) was the most common pathogenic parasite. all urines were parasite free. the low infection rate with gardia could be explained by the good physical and nutritional status and better sanitary and living conditions.	1989	2708849
relationship of serum ferritin and iron concentrations and serum total iron-binding capacity to nonheme iron stores in dogs.	the relationships of various iron-related analytes were evaluated in 95 dogs. liver and spleen nonheme iron content was determined coulometrically on acid-digested tissue specimens. serum iron concentration and total iron-binding capacity also were measured coulometrically, whereas serum ferritin concentration was measured by elisa. significant (p less than 0.0002) correlation was found between serum ferritin concentration and nonheme iron stores. significant correlation was not found between no ...	1989	2719382
some physico-chemical properties of the rigid form of the sendai virus nucleocapsid.	the effect of some dissociation agents (sds, beta-mercaptoethanol, urea, edta) on the rigid form of the sendai virus nucleocapsid was studied. polyacrylamide gel electrophoresis in the presence of lytic mixture (1% sds, 2% beta-mercaptoethanol, 5 m urea, for 2 min at 100 degrees c) revealed two types of polypeptide subunits (mol. wts. 46,000 and 14,000), as well as the dissociation in the presence of 0.1% sds only. the edta treatment leads to a disorganization of the protein part (10(-2) m) or o ...	1989	2556836
decreased 3 alpha-androstanediol glucuronide levels in plasma and random urines in male pseudohermaphroditism caused by 5 alpha-reductase deficiency.	concentrations of 3 alpha-diol glucuronide (3 alpha-diol g) in plasma and/or random urine samples were determined in seven subjects with familial male pseudohermaphroditism (fmp) due to 5 alpha-reductase deficiency (5 alpha-rd). all subjects were natives of an isolated turkish village with a high incidence of consanguineous marriage. a specific and sensitive antibody to 3 alpha-diol was used for radioimmunoassay of 3 alpha-diol g after hydrolysis and chromatographic purification. the mean plasma ...	1989	2770532
p-glycoprotein and progesterone receptor in human breast cancer.		1990	1974809
[electron microscopic cytochemical study on lectin binding sites in the epiphyseal cartilage-plate of rabbit tibia].	lectin is a common name given to a certain group of hemagglutinating proteins found primarily in plant seeds, which bind specifically to the branching sugar molecules of glycoproteins and glycolipids of the surface of the cells. the author selected 3 kinds of lectin among others. those were concanavalin a (con a) which specifically binds to the alpha-d-mannose, wheat germ agglutinin (wga) which binds to n-acetyl glucosamine, and peanut agglutinin (pna) which binds to beta-d-galactosamine. the lo ...	1990	2134934
lack of pqq dependent glucose dehydrogenase apoenzyme in some acinetobacter spp.	seventy four asaccharolytic strains of acinotobacter were tested for the presence of pqq dependent glucose dehydrogenase apoenzyme. this apoenzyme could not be detected in any of five strains of acinetobacter haemolyticus; it was also lacking in one strain of each a. junii and a. lwoffi.	1990	2176072
structural specificity for biological activity of trichostatin a, a specific inhibitor of mammalian cell cycle with potent differentiation-inducing activity in friend leukemia cells.	biological activities of four chemically synthesized trichostatin-related compounds, (r)-trichostatin a, (s)-trichostatin a, (r)-trichostatic acid, and (s)-trichostatic acid, were investigated. assays of differentiation-inducing activity in friend leukemia cells and g2-arresting activity in the cell cycle of normal rat fibroblast cells were used as monitoring systems for comparing the bioactivities of these compounds. the results clearly showed that both of the enantiomers of trichostatic acid h ...	1990	2211374
ethanol reduces tolerance, sensitization, and up-regulation of d2-receptors after subchronic haloperidol.	to study the interrelationships between dopamine d2-receptor density and behavioral responses after chronic treatment with neuroleptics female wistar rats received haloperidol (hp; 14 mg/l), ethanol (etoh; 5 vol.%), a combination of both, or tap water as drinking fluids for one or two weeks. mean intake doses ranged between 1.28 and 1.48 mg/kg/day (hp) and between 3.7 and 4.8 g/kg/day (etoh). hp administered for one or two weeks raised the number of [3h]spiroperidol binding sites in the striatum ...	1990	2217521
[effect of enalapril on heart rate, arterial blood pressure and exocrine pancreatic secretion in the alert dog].	a changed exocrine pancreatic secretion could be a pathogenetic factor of an acute pancreatitis after administration of angiotensin-converting enzyme (ace) inhibitors. in six conscious dogs with gastric and duodenal thomas fistulas we studied the effect of an intravenous (iv.) bolus injection of 10 mg enalaprilat, an intraduodenal (id.) bolus injection of 20 and 40 mg enalapril (e.), and 0.15 m nacl (20 ml iv., resp., id.) on pancreatic bicarbonate- and protein output in response to secretin (20 ...	1990	2238764
analysis of immunosuppressive molecules associated with murine in vitro fertilized embryos.	supernatants from mouse in vitro fertilized (ivf) oocyte cultures may suppress in vitro lymphocyte proliferation stimulated by concanavalin a (sigma, st. louis, mo). supernatants conditioned by incubation with mouse epididymal sperm alone were even more inhibitory. thin-layer chromatography suggested the polyamines spermine in sperm and spermine plus spermidine in ivf embryo supernatants were responsible. putrescine was not suppressive. in vitro fertilized oocytes from old cba/j-strain mice (gre ...	1990	2245843
intermolecular recombination of the c-myb proto-oncogene coding sequences in chicken: clues for a mechanism.	the physical separation of c-myb coding exons on two different chromosomes, both in chicken and human, indicated that intermolecular recombination events might occur in higher eucaryotes. we present evidence in this paper suggesting that the expression of "antisens" mrna species could be involved in the regulation of the intermolecular recombination process.	1990	2129112
vibrios associated with red tides caused by mesodinium rubrum.	vibrios were isolated from red tides caused by mesodinium rubrum and also throughout the year in the ria de pontevedra, spain. the isolates were grouped into 14 phena by numerical toxonomy. strains associated with red tides were restricted to four phena: phena i and ii were vibrio alginolyticus, and phena iii and iv were vibrio tubiashii and vibrio anguillarum, respectively. v. anguillarum-like strains (phena v through xi) predominated throughout the year outside the red tide areas. cytotoxicity ...	1990	2268167