Publications

biosynthesis of methylheptadecanes in anabaena variabilis. in vitro incorporation of s-(methyl- 14 c)adenosylmethionine.		1972	4402740
further studies on the oxygen-reducing system of anabaena variabilis.		1972	4622735
characteristics of the nitrogenase system of the blue-green alga anabaena cylindrica.		1972	4623674
[photoinduced ph changes in cell suspensions of the blue-green algae anabaena variabilis and synechosystis aquatilis].		1972	4625326
[a-1 (l)-cyanophage of the blue-green alga anabaena variabilis].		1972	4625591
site of nitrogenase activity in the blue-green alga anabaena sp. l-31.		1972	4626450
the influence of canavanine, oxygen, and urea on the steady-state levels of nitrogenase in anabaena flos-aquae.		1972	4628176
the electrophoretic characterization of ribosomes from the blue-green alga anabaena flos-aquae.		1972	4628179
the activity of deoxyribonucleic acid polymerase in some species of algae.	1. the activities of dna polymerase preparations from the algae euglena gracilis, chlamydomonas reinhardtii, chlorella pyrenoidosa, anabaena variabilis and anacystis nidulans were measured. the blue-green algae anabaena and anacystis contain a 5-20-fold higher activity of the enzyme than do the green algae. dna polymerases from the blue-green algae show a ph optimum of 9 and prefer a relatively low mg(2+) concentration (1-3mm). dna polymerases from the green algae, however, display a ph optimum ...	1972	4629975
structural studies on the glycolipids from the envelope of the heterocyst of anabaena cylindrica.		1973	4631370
pathways of glycollate metabolism in the blue-green alga anabaena cylindrica.		1973	4363485
[nitrogen excretion by nitrogen fixing blue-green algae. i. growth of anabaena cylindriea and nostoc muscorum in various media, at different temperatures, and under light of different wavelengths (author's transl)].		1973	4272180
[temperate cyanophage a-4 (l) of the blue-green alga anabaena variabilis].		1973	4209794
action of lysozyme on anabaena ambigua rao.		1973	4196488
study of the extracellular polysaccharides produced by a blue-green alga, anabaena flos-aquae a-37.		1973	4197121
[detection of the a-1 virus of the blue-green alga anabaena variabilis in the kremenchug artificial reservoir].		1973	4207987
pattern formation in the blue-green alga, anabaena. i. basic mechanisms.		1973	4198321
the effect of chloramphenicol on the production of cyanophycin granule polypeptide in the blue green alga anabaena cylindrica.		1973	4200161
alteration in heterocyst pattern of anabaena produced by 7-azatryptophan.		1973	4202839
nitrogen fixation by anabaena cylindrica. ii. nitrogenase activity during induction and aging of batch cultures.		1973	4202933
influence of certain pesticides on microorganisms. influence of ddt on the synthesis of the particular amino acids in chlorella vulgaris and anabaena cylindrica.		1973	4203421
pattern formation in the blue-green alga anabaena. ii. controlled proheterocyst regression.		1973	4203959
[some characteristics of the behavior of the blue-green alga anabaena variabilis under conditions of darkness].		1973	4205729
glutamine synthetase of the nitrogen-fixing alga anabaena cylindrica.		1973	4144960
measurement of the cyanophycin granule polypeptide contained in the blue-green alga anabaena cylindrica.	an assay was developed to determine the amount of cyanophycin granules in blue-green algae. the amount of this polypeptide in cells of anabaena cylindrica was measured as a function of culture age and was compared with changes in other proteinaceous cellular components. the data presented support the notion that the cyanophycin granule is a cellular nitrogen reserve.	1973	4197270
activities of enzymes of the oxidative and the reductive pentose phosphate pathways in heterocysts of a blue-green alga.	preparations of heterocysts of anabaena cylindrica lemm. had 7- to 8-fold higher activities of glucose 6-phosphate dehydrogenase and 6-phosphogluconate dehydrogenase, 2-fold more hexokinase activity, and 0.02 to 0.06 times as much ribulose diphosphate carboxylase and glyceraldehyde 3-phosphate dehydrogenase activities as did whole filaments per milligram soluble protein in cell-free extracts. time courses of solubilization of glucose 6-phosphate dehydrogenase activity indicated that heterocysts ...	1973	16658588
polypeptide composition of photosynthetic membranes from chlamydomonas reinhardi and anabaena variabilis.	anabaena variabilis, a blue-green alga lacking chlorophyll b, shows an absence of the major 22 and 24 kilodalton polypeptides which are present in the photosynthetic membranes of chlamydomonas reinhardi and higher plants. these data are consistent with other investigations which have shown that these polypeptides are associated with chlorophyll b in the chloroplasts of higher plants, and indicate the presence of a light harvesting chlorophyll-protein complex in higher plants which contains the c ...	1974	16658789
the azolla, anabaena azollae relationship: i. initial characterization of the association.	cultures of azolla caroliniana willd. free of the symbiotic blue-green alga, anabaena azollae, were obtained by treatment of azolla fronds with a regimen of antibiotics. these symbiontfree plants can be maintained only on medium containing a combined nitrogen source.morphological aspects of the symbiotic association show the confinement of the anabaena azollae within the leaf cavity of the azolla. procedures were established for the isolation of pure preparations of anabaena azollae and azolla c ...	1974	16658796
the azolla, anabaena azollae relationship: ii. localization of nitrogenase activity as assayed by acetylene reduction.	anaerobic (microaerophilic) acetylene reduction by azolla caroliniana willd. was dependent on light and saturated at approximately 450 foot candles. maximum rates of acetylene reduction were 60 nmoles/mg chlorophyll minute. however, rates of 25 to 30 nmoles/mg chlorophyll minute were more common.the growth of azolla for 35 days with nitrate or urea as a nitrogen source decreased the rate of acetylene reduction approximately 30% compared to controls grown on nitrogen. prolonged growth on nitrate ...	1974	16658797
hydrogen evolution by nitrogen-fixing anabaena cylindrica cultures.	actively growing, nitrogen-fixing cultures of the blue-green alga anabaena cylindrica can simultaneously evolve hydrogen and oxygen frolni water and light. hydrogen evolution was strongly inhibited by n(2) but only slightly by co or o(2), characteristics of the nitrogenase reaction in the heterocysts of anabaena cylindrica. we suggest that this reaction has potential use in solar energy conversion.	1974	17791443
uridine diphosphate galacturonate 4-epimerase from the blue-green alga anabaena flos-aquae.		1974	4150793
in vitro protein synthesis and measurement of the stability of messenger rna in the blue-green alga, anabaena variabilis.		1974	4207060
autoradiographic localization of 13n after fixation of 13n-labeled nitrogen gas by a heterocyst-forming blue-green alga.	(13)n, generated by proton bombardment of (13)c powder, is rapidly and easily converted to (13)n-n(2), 0.01 atm pressure, ca. 10 mci/ml, by automated dumas combustion. (13)n fixed (as (13)n-n(2)) by algal filaments was localized by an autoradiographic technique which permits track autoradiography with isotopes having short half-lives. our findings show directly that a minimum of about 25% of the n(2) fixation by intact, aerobically grown filaments of anabaena cylindrica is carried out by the het ...	1974	4208073
nitrogen metabolism and ultrastructure in anabaena cylindrica. i. the effect of nitrogen starvation.		1974	4209227
properties and function of the pyruvate: ferredoxin oxidoreductase from the blue-green alga anabaena cylindrica.		1974	4209549
pyruvate and nitrogenase activity in cell-free extracts of the blue-green alga anabaena cylindrica.		1974	4218098
lipopolysaccharide containing l-acofriose in the filamentous blue-green alga anabaena variabilis.	for the first time, an o-antigenic lipopolysaccharide (lps) has been isolated from a filamentous blue-green alga (anabaena variabilis). it was extractable with phenol-water, resulting in extraction of the bulk of the lps into the phenol phase. the polysaccharide moiety of this lps consists of l-rhamnose, its 3-o-methyl ether l-acofriose, d-mannose, d-glucose, and d-galactose. l-glycero-d-mannoheptose and 2-keto-3-deoxyoctonate, the two characteristic sugar components of enteric lps, and phosphat ...	1974	4218229
[electron microscopic study of the development of the cyanophage a-1l in cells of the blue-green alga anabaena variabilis].		1974	4218858
the metabolism of glucose by heterocysts and vegetative cells of anabaena cylindrica.		1974	4280889
nitrogen metabolism and ultrastructure in anabaena cylindrica. ii. the effect of molybdenum and vanadium.		1974	4372965
[the cellular ultrastructure of obligate phototrophic blue-green alga anabaena variabilis in cultures dying in the dark].		1974	4375246
alanine dehydrogenase of the n2-fixing blue-green alga, anabaena cylindrica.	the l-alanine dehydrogenase (adh) of anabaena cylindrica has been purified 700-fold. it has a molecular weight of approximately 270,000, has 6 sub-units, each of molecular weight approximately 43,000, and shows activity both in the aminating and deaminating directions. the enzyme is nadh/nad+ specific and oxaloacetate can partially substitute for pyruvate. the kampp for nad+ is 14 mum and 60 mum at low and high nad concentrations respectively.	1975	4043
effects of l-methionine-dl-sulphoximine on the assimilation of newly fixed nh3, acetylene reduction and heterocyst production in anabaena cylindrica.		1975	239712
toxicology and pharmacological action of anabaena flos-aquae toxin.	calves, rats, ducks, and goldfish given lethal oral doses of bacteria-free lyophilized cell suspensions of toxic anabaena flos-aquae died as a result of respiratory arrest. experiments with selected animals and pharmacological preparations showed that the main effect of the toxin was production of a sustained postsynaptic depolarizing neuromuscular blockade.	1975	803708
mutants of anabaena cylindrica altered in heterocyst spacing.	nitrosoguanidine induced mutants of anabaena cylindrica have been obtained, which are altered in heterocyst spacing. in the wild type organism the pattern is composed of single intercalary heterocysts. the mutant patterns fall into several classes: those with only terminal heterocysts, with both terminal and intercalary heterocysts, with groups of heterocysts and those totally lacking heterocysts. the mutants are described in detail, and the various pattern modications are interpreted in terms ...	1975	807173
effect of pesticides on blue-green algae and nitrogen-fixation.	the effects of the pesticides, amitrol, a derivative of amitrol (viz. 3,5-diamino-1,2,4-triazole), diquat, paraquat, linuron, mcpa, malathion, and monuron, were studied on the nitrogen-fixing algae, anabaena cylindrica, aulosira sp., calothrix elenkenii. chlorogloeae frischii, cylindrospermum muscicola, nostoc sp. from collema tenax, nostoc muscorum tolypothrix tenuis, and westiellopsis sp. in general, two types of response were discernible; an initial period of depression succeeded by an increa ...	1975	808179
[electron microscopic and biochemical study of spheroplasts of the blue-green alga anabaena variabilis].	electron microscopy of the spheroplasts of the blue-green alga anabaena variabilis revealed damages induced by lysozyme. biochemical analysis confirmed the data of electron microscopy that the spheroplasts had lost partly the cytoplasmic content of the cells. dna was preserved in the spheroplasts though the nucleoid was not detected by electron microscopy.	1975	808685
[comparative study of the functions of the spheroplasts and cells of the blue-green alga anabaena variabilis].	the effect of light was studied with the spheroplasts of the blue-green alga anabaena variabilis. contrary to the intact cells, the spheroplasts did not synthesize nucleic acids and pigments in the light. these components of the spheroplasts were decomposed in the light, and the remaining chlorophyll was incapable of luminescence. the rate of oxygen uptake increased upon the incubation of the spheroplasts in the light. changes of the functions induced by lysozyme in the cells of a. variabilis ar ...	1975	808691
nuclear magnetic resonance studies of the copper binding sites of blue copper proteins: oxidized, reduced, and apoplastocyanin.	proton nuclear resonance spectra at 250 mhz of plastocyanins from spinach (spinacia oleracea) and a blue green alga (anabaena variabilis) are reported. spectra of the reduced plastocyanins contain well-resolved peaks from slowly exchangeable n-h, histidine c2-h tyrosine ring, peptide alpha-ch, and high-field protons. the widths of these peaks indicate that the plastocyanins are monomeric. when the plastocyanins are oxidized, several changes in the spectra are observed including disappearance of ...	1975	809054
[change in the atp content in the cells of anabaena variabilis].	the content of atp in the cells of anabaena variabilis does not change during one day in the darkness as compared to that in the cells grown in the light, this suggesting the endogenous energy reserve of the cell. the level of atp gradually decreases when the cells are incubated in the darkness during 25 days, but does not reach zero. the content of atp increases for a short period in the light in the cells that have been incubated in the darkness during several days (up to 7 days). on the contr ...	1975	809639
assimilation of citric acid and adipic acid by the blue-green alga anabaena variabilis.	the assimilation of [1,6-14c] citric acid and [1,6-14c] adipic acid by the blue-green alga anabaena variabius was studied in the dark and in the light. citric acid was assimilated in the dark and in the light but adipic acid showed only limited assimilation in the dark. in the light the assimilation of adipic acid did not enhance the growth of the alga at a concentration of 2.85 x 10(-8) m. growth was inhibited at adipic acid concentrations greater than 10(-3) m. analysis of the products of adip ...	1975	810235
production of nutritionally-deficient mutants of the axenic blue-green alga anabaena flos-aquae nrc-44-1 by ultraviolet irradiation.		1975	810978
the effect of pyruvate on nitrogenase activity in the blue-green alga anabaena cylindrica.	exogenous pyruvate added to cultures of the blue-green alga, anabaena cylindrica stimulated nitrogenase activity (measured by acetylene reduction) only in the dark under low po2 (0.05 atmospheres). under aerobic conditions or in the light, stimulation was absent and replaced by an inhibition of activity above 5 mm added pyruvate. the curve of nitrogenase activity versus oxygen concentration had a similar maximal value of ethylene production with, or without added pyruvate, but in the presence of ...	1975	811189
division of heterocysts in the blue-green alga anabaena doliolum bharadwaja.		1975	812271
prokaryote-eukaryote relationship and the amino acid sequence of plastocyanin from anabaena variabilis.	the amino acid sequence of plastocyanin from the prokaryotic blue-green alga anabaena variabilis was determined. the protein consists of a single polypeptide chain of 105 residues. the amino acid sequence of the plastocyanin was compared with that of the eukaryotic green alga chlorella fusca and with those of higher-plant plastocyanins. the considerable similarity between the prokaryotic and eukaryotic plastocyanins is discussed. detailed evidence for the sequence of the protein has been deposit ...	1975	812489
the initial organic products of fixation of 13n-labeled nitrogen gas by the blue-green alga anabaena cylindrica.		1975	812496
letter: toxicity to livestock of the blue-green algae anabaena circinalis.		1975	816341
[characteristics of oxygen metabolism in the obligate phototrophic blue-green alga anabaena variabilis in darkness].	the rate of endogenous respiration of the cells of anabaena variabilis in the mineral medium in the darkness decreases gradually during 30 to 40 days and is then maintained at a low level. the rate of oxygen uptake was the same in the cells grown in aerobic and anaerobic conditions in the darkness. the ability for oxygen evolution in the light is maintained in the anaerobic cells for a longer time, and the biomass yield and the content of phycocyanin in the cells decrease at a lower rate in anae ...	1975	818482
the intracellular localization of the glycollate-oxidising enzyme of anabaena cylindrica.		1975	808429
allophycocyanin b (lambdamax 671, 618 nm): a new cyanobacterial phycobiliprotein.	a hitherto undescribed red fluorescent phycobiliprotein (maximum emission at congruent to 680 nm), characterized by long wavelength absorption maxima in the visible region at 671 nm (xi = 172000 m(-1).cm(-1) per monomer of mol. wt. 30600)and 618 nm, has been purified to homogeneity from unicellular cyanobacterium, synechococcus sp., and from a filamentous cyanobacterium, anabaena variabilis. the name allophycocyanin b has been proposed for the new protein. a. variabilis allophycocyanin b is ch ...	1975	808186
regulation of glucose 6-phosphate dehydrogenase in blue-green algae.	glucose 6-phosphate dehydrogenase (ec 1.1.1.49) has been partially purified from anacystis nidulans and anabaena flos-aquae by means of ammonium sulfate fractionation and exclusion gel chromatography and the kinetic properties determined.glucose 6-phosphate dehydrogenase from these blue-green algae exhibits michaelis-menten kinetics at ph 6.7. at this ph, km values of 0.37 mm for glucose 6-phosphate and 10 mum for nadp were determined. at a ph above 7.4, the enzyme exhibits sigmoidal kinetics wi ...	1975	16659143
simultaneous measurement of oxygen and hydrogen exchange from the blue-green alga anabaena.	two clark-type polarographic electrodes were used to measure simultaneous h(2) and o(2) exchange from three species of the blue-green alga anabaena. maximum h(2) photoevolution from n(2)-fixing cultures of anabaena required only the removal of dissolved o(2) and n(2); no adaptation period was necessary. no correlation of h(2) photoproduction with photosynthetic o(2) evolution, beyond their mutual light requirement, was found. hydrogen photoevolution has the following characteristics in common wi ...	1976	16659547
azolla-anabaena azollae relationship: iv. photosynthetically driven, nitrogenase-catalyzed h(2) production.	the water fern, azolla caroliniana willd., containing the symbiotic, heterocystous blue-green alga, anabaena azollae, has been studied under various growth conditions to characterize its light-dependent production of h(2). the response of h(2) production to n(2) and c(2)h(2) and the absence of a differential effect of m-chlorocarbonyl cyanide phenylhydrazone on h(2) production and c(2)h(2) reduction, coupled with the parallel inhibition of both processes by dcmu imply that the production of h(2) ...	1976	16659630
altered nitrogenous pools induced by the azolla-anabaena azolla symbiosis.	the free amino acid and ammonia pools of azolla caroliniana were analyzed by quantitative column chromatography on columns capable of separating all of the nitrogenous constituents normally found in physiological fluids. comparisons were made of plants containing symbiotic algae and grown on nitrogen-free media, plants grown on media containing nitrate, and algae-free plants also grown on nitrate media. the major feature of the data was a very high level of intracellular ammonia found in plants ...	1976	16659770
the incorporation of nitrogen into products of recent photosynthesis in anabaena cylindrica lemm.	in vivo tracer studies with 14c have been performed to help determine pathways of incorporation of newly assimilated nitrogen into n2-fixing cells of anabaena cylindrica. after photosynthesis in ar: o2:14co2 for 30 min, the addition of n2 or nh+4 resulted in increased rates of 14co2-incorporation both in the light and dark, and in increased incorporation of 14c into amino acids at the expense of sucrose and sugar phosphates. evidence of enhanced sucrose catabolism and increased pyruvate kinase a ...	1976	814876
photosynthetic electron transport, atp synthesis and nitrogenase activity in isolated heterocysts of anabaena cylindrica.	isolated heterocysts of the n2-fixing blue-green alga anabaena cylindrica contain the photosystem i components p-700, bound and soluble ferredoxins and ferredoxin-nadp reductase. they also show photosystem i activity being able to photoreduce both methylviologen and nadp when ascorbate + dichlorophenol-indophenol acts as reductant. they photophosphorylate (64 munol atp produced/mg chlorophyll a/h) and carry out oxidative phosphorylation (8.7 munol atp produced/mg chlorophyll a/h). ninety per cen ...	1976	813780
[photodestruction of the blue-green alga anabaena variablis].	the effect of light on the cells was studied with the obligate phototrophic blue-green alga anabaena variabilis after its incubation in the dark. incubation of the alga in the dark during two weeks was not essential for the cell reproduction systems in the light. incubation in the dark during 3-4 weeks caused damages in the systems which were however reversible and repaired in the light. longer incubation of the cells in the dark results in irreversible damages of the systems protecting them fro ...	1976	827666
[hydrogen production by the cyanobacterium anabaena variablis in the light].	light of low intensity (less than or equal to 25-10(5) erg-cm(-2)-sec(-1)) stimulates hydrogen production by cell suspensions of anabaena variabilis in the presence of glucose, pyruvate or formate. the maximum rate of hydrogen production in the presence of these substrates was observed at light intensities of 650, 1400 and 2250 erg-cm(-2)-sec(-1), respectively. the rate of oxygen production by the cells increases while the rate of hydrogen evolution decreases with increase in light intensity (2. ...	1976	827669
the polysaccharides from heterocyst and spore envelopes of a blue-green alga. methylation analysis and structure of the backbones.	lindberg's combined gas chromatography-mass spectrometry techniques for the analysis of partially methylated alditol acetate sugar derivatives were used to study the structures of polysaccharides from the envelopes of heterocysts and spores of anabaena cylindrica. polysaccharides from both envelopes are highly branched. glucose, mannose, galactose, and xylose are at terminal positions, whereas glucose and mannose are at internal positions in these polymers. the molar percentages of the 11 partia ...	1976	818084
the biosynthesis of multi-l-arginyl-poly(l-aspartic acid) in the filamentous cyanobacterium anabaena cylindrica.	the cyanobacteria produce multi-l-arginyl-poly (aspartic acid), a high molecular weight (mr=25 000-125 000) branched polypeptide consisting of a poly(aspartic acid) core with l-arginyl residues peptide bonded to each free carboxyl group of the poly(aspartic acid). an enzyme which will elongate arg-poly(asp) has been isolated and purified 92-fold from the filamentous cyanobacterium anabaena cylindrica. the enzyme incorporates arginine and aspartic acid into arg-poly(asp) in a reaction which requi ...	1976	2311
crystalline structure of the gas vesicle wall from anabaena flos-aquae.		1976	184286
the fine structure of a tri-lamellar body in various species of anabaena.		1976	184493
epr spectra of photosystem i constituents in heterocyst preparations from anabaena cylindrica.		1976	186323
carbon dioxide assimilation in blue-green algae: initial studies on the structure of ribulose 1,5-bisphosphate carboxylase.	d-ribulose 1,5-bisphosphate carboxylase was purified from the blue-green alga anabaena cylindrica (lemm) by procedures involving acid precipitation, ammonium sulfate fractionation, and sephadex g-200 gel filtration. the enzyme was homogeneous by the criterion of polyacrylamide disc gel electrophoresis and was a multimer of a single-size polypeptide chain of 54,000 daltons. the carboxylases from four species of blue-green algae (anabaena, nostoc strain mac, agmenellum quadruplicatum strain pr-6, ...	1976	812868
measurement of an inhibitory zone.	an inhibitory zone mechanism generates the heterocyst pattern in anabaena. these inhibitory zones can be measured; we find that they extend about five cells on either side of a heterocyst. we can use our observations to predict with reasonable accuracy which cells in the filaments will differentiate into heterocysts.	1976	814620
isolation, growth and nitrogen fixation of a gas vacuole-less mutant of the blue-green alga anabaena aphanizomenoides.	mutants with loss of gas vacuoles (gvl-) were isolated from a clonal population of the gas vacuole containing (gvl+) blue-green alga anabaena aphanizomenoides with a spontaneous mutation frequency of 1.7 x 10(-3) in nitrogen-free media. growth and nitrogen fixation of the mutant were slow when compared to the gvl+ parent. both the parent and mutant filaments tolerated 0.02 microgram/ml of streptomycin, and higher concentrations were lethal. the reversion of the mutant towards the parent did not ...	1976	829596
[hydrogen metabolism in anabaena variabilis in the dark].	cells and extracts of the cyanobacterium anabaena variabilis are capable of hydrogen absorption in the dark in the presence of h-acceptors with various redox potentials. preliminary adaptation of the cells to anaerobic conditions has no effect on the process. a. variabilis can also evolve hydrogen in the dark. reduced methylviologen (rmv), formiate, pyruvate, and glucose may be substrates for hydrogen evolution by the cells. the extracts evolve hydrogen in the presence of rmv, benzylviologen, az ...	1976	824526
the fine structure of striated microtubules and sleeve bodies in several species of anabaena.		1976	825659
cyanobacterial dna-binding protein related to escherichia coli hu.	a dna-binding protein has been isolated from two cyanobacteria (blue-green algae): anabaena sp and aphanocapsa sp. it has a molecular weight as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis of about 10,000, is rich in lysine and arginine, and lacks tyrosine, tryptophan, and cysteine. the proteins from both strains show immunological identity with a similar dna-binding protein from escherichia coli, when tested by immunodiffusion with an antiserum prepared against the e. ...	1976	819928
atp pools and transientss in the blue-green alga, anabaena cylindrica.	anabaena cylindrica grown in steady state continuous culture has an extractable atp pool, measured on the basis of the luciferin-luciferase assay of 165 +/- 35 nmoles atp mg chla-1. this pool is maintained by a dynamic balance between the rate of atp synthesis and the rate of atp utilization. phosphorylating mechanisms which can maitain the pool in the short term are total photophosphorylation, cyclic photophosphorylation and oxidative phosphorylation. the alga can maintain its atp pool by switc ...	1976	821448
pathway of nitrogen metabolism after fixation of 13n-labeled nitrogen gas by the cyanobacterium, anabaena cylindrica.	methods have been developed for identifying the pathway of assimilation of n2-derived nitrogen. the products of fixation of 13n-labeled nitrogen gas ([13n]n2), and the distribution of 13n within glutamine, were determined after short periods of labeling (approximately 1 to 120 s) and also in pulse-chase experiments. ammonia, the amide nitrogen of glutamine, and the alpha-amino nitrogen of glutamate, in that order, were the first observed products of fixation of n2 by the cyanobacterium (blue-gre ...	1976	821946
[ribosomes of the blue-green alga anabaena variabilis. sedimentation, density characteristics and analysis of ribosomal rna].	ribosomes and rrnas were isolated from cells of green-blue alga anabaena variabilis. the sedimentation properties of the ribosomes as well as density, molecular weights and nucleotide composition of rrnas were determined. the ribosomes were found to have the s20,w value equal to 67.2+/-0.4s, whereas those of the ribosome subunits were 48.6+/-0.8 and 29.5+/-1.1s. the buoyant density of the ribosomes in cscl was 1.641+/-+/-0.002 g/cm3; a calculated relative protein content was 35%. the molecular w ...	1976	823982
heterocyst and nitrogenase development in anabaena cylindrica.	the differentiation of filaments of a continuous culture of anabaena cylindrica after removal of fixed nitrogen has been examined. rapid development of proheterocysts (4-5 h) and mature heterocysts (14 h) in an ordered sequence was observed; the development of the latter was concomitant with the onset of nitrogenase activity. commitment times of 2-3 h (proheterocyst) and 5-0 (heterocyst) were measured. evidence is presented that shows that heterocyst development, rather than any product of heter ...	1976	824402
properties of heterocysts isolated with colloidal silica.	a method is described for the isolation of heterocysts that are virtually free of contaminating cell debris after sonication of aerobically grown anabaena 7120. isolated heterocysts reduced acetylene in a light-dependent process in the absence of exogenously provided atp; heterocysts supplied with atp and na2s2o4 reduced acetylene slowly in the dark but still showed a marked light activation. nitrogenase activity was greatest in fractions containing intact heterocysts. up to 13% of the activity ...	1976	5981
"synthetical" aiptasia mutabilis rapp (coelenterata) (author's transl).	after being cultivated in the dark for some months and after being fed with food free of carotenoids during the time aiptasia mutabilis (coelenterata) loses its symbiontic algae (rich in brown fucoxanthin) and becomes transparent white. this disarranged symbiosis may be regenerated under light cultivation by adding different species of chlorophyceae (chlorella, dunaliella) and chrysophyceae (ochromonas, cyclotella), but not of cyanophyceae (anabaena, nostoc, oscillatoria, anacystis). - this chan ...	1976	8888
isolation and characterization of two sequence-specific endonucleases from anabaena variabilis.	two endonucleases, avai and avaii, were isolated from anabaena variabilis on the basis of their ability to make a limited number of breaks at specific points in bacteriophage lambda dna. neither enzyme has cofactor requirements beyond mg2+. endonuclease avai makes eight breaks in the phage lambda chromosome at which the 5'-terminal sequence is ppy-c-g-n. avaii endonuclease cuts phage lambda dna more extensively, yielding fragments with the 5'-terminal sequence g-t-c-n or g-a-c-n. neither enzyme ...	1976	11780
heterotrophic micro- and macrocultures of a nitrogen-fixing cyanobacterium.	anabaena variabilis can be cloned in the dark from fragments with one and few cells, with an efficiency of about 40%, on the nitrogen-free medium of allen and arnon solidified with 0.5% agarose and supplemented with 5 mm fructose. the organism can be grown exponentially (tau2 approximately or equal to 36 h) in fermentor cultures in the dark, fixing n2, to a density of greater than 10 g dry weight/l.	1976	828022
the fine structure of a microplate-microtubule array, microfilaments and polyhedral body associated microtubules in several species of anabaena.	a microplate-microtubule array was observed in anabaena sp. (b-378). this structure consists of an arched plate, about 8 nm thick, and various microtubules, 12 nm in diameter and 50 nm long, arranged in rows. the microtubules project at right angles from one side of the plate into the cytoplasm or towards the plasma membrane. up to twelve microplate-microtubule arrays were observed in a single section of a cell. microfilaments, about 2.8 nm in diameter and of undetermined length, were observed i ...	1976	828027
[metabolism and destruction of the blue-green alga anabaena variablis in the dark].	anabaena variabilis remains viable in the dark, which is accompanied with intensive utilization of the reserve polysaccharide by the cells. the content of protein and rna increases in the cells and incomplete cellular division occurs at the beginning of the culture incubation in the dark. destruction of the intracellular protein and rna begins before all the reserve polysaccharide has been utilized. during the whole period of incubation of the alga in the dark, structural integrity of the cells ...	1976	826765
characterization and structural properties of the major biliproteins of anabaena sp.	studies are presented of the biliproteins of anabaena sp. this filamentous cyanobacterium contains three major biliproteins. whereas two of these, c-phycocyanin and allophycocyanin, are common to all cyanobacteria, the third, phycoerythrocyanin (gammamax approximately 568 nm) has hitherto not been described and its distribution among cyanobacteria appears to be limited. anabaena variabilis and anabaena sp. 6411 allophycocyanin, c-phycocyanin, and phycoerythrocyanin were purified to homogeneity a ...	1976	828020
formation of glutamine from 13nammonia, 13ndinitrogen, and 14cglutamate by heterocysts isolated from anabaena cylindrica.	a method is described for the isolation of metabolically active heterocysts from anabaena cylindrica. these isolated heterocysts accounted for up to 34% of the acetylene-reducing activity of whole filaments and had a specific activity of up to 1,560 nmol of c2h4 formed per mg of heterocyst chlorphyll per min. activity of glutamine synthetase was coupled to activity of nitrogenase in isolated heterocysts as shown by acetylene-inhibitable formation of 13nnh3 and of amidelabeled 13nglutamine form 1 ...	1977	14927
penicillinase (beta-lactamase) formation by blue-green algae.	beta-lactamase (penicillinase) activity was found in a number of strains of blue-green algea. in some cases, this enzyme permitted algae to overcome the inhibitory effects of penicillin. production and localization of beta-lactamase were studied in a unicellular species, coccochloris elabens (strain 7003), and in a filamentous, nitrogen-fixing anabaena species (strain 7120). when cells were grown in a neutral medium with nano3 as n source, the ph rose during growth; at a ph of about 10, most of ...	1977	15530
nitrogen and ammonia assimilation in the cyanobacteria: purification of glutamine synthetase from anabaena sp. strain ca.	glutamine synthetase was purified from the cyanobacterium anabaena sp. strain ca, a newly isolated marine organism. this organism grows rapidly under nitrogen-fixing conditions and therefore is ideally suited for studies concerning cyanobacterial nitrogen metabolism. studies were conducted to optimize the production of glutamine synthetase by anabaena ca. the highest specific activities were obtained from cells grown in the presence of atmospheric n(2) or kno(3) (13 mm); when nh(4)cl was used as ...	1977	21167
the azolla-anabaena azzolae symbiosis.		1977	21650
estimation of chlorophyll a distribution in the photosynthetic pigment systems i and ii of the blue-green alga anabaena variabilis.	chlorophyll a distribution in pigment systems i and ii was estimated with the blue-green alga anabaena variabilis by two methods: first, with intact cells using delayed light emission as an index reaction; second, by measuring the 2,6-dichlorophenolindophenol-hill reaction and the cytochrome c photooxidation in membrane fragments. the first estimation indicated that 0.053+/-0.014 of total chlorophyll a functions as a component of pigment system ii, and the second method 0.086+/-0.012. though the ...	1977	66064
hydrogen production by nitrogen-starved cultures of anabaena cylindrica.	nitrogen-starved cultures of the alga anabaena cylindrica 629 produced hydrogen and oxygen continuously for 7 to 19 days. hydrogen production attained a maximum level after 1 to 2 days of starvation and was followed by a slow decline. the maximum rates were 30 ml of h2 evolved per liter of culture per h or 32 mul of h2 per mg of dry weight per h. in 5 to 7 days the rate of h2 evolution by the more productive cultures fell to one-half its maximum value. the addition of 10(-4) to 5 x 10(-4) m ammo ...	1977	402109
macromolecular composition of spores from the filamentous cyanobacterium a nabaena cylindrica.	spores were isolated from the filamentous cyanobacterium anabaena cylindrica, and their deoxyribonucleic acid, ribonucleic acid, and protein compositions were determined.	1977	402350
action of alkylating agents on nitrogen fixation by clones of anabaena doliolum bharadwaja.		1977	403090
isolation and preliminary characterization of auxotrophs of a filamentous cyanobacterium.	auxotrophic mutants of the filamentous cyanobacterium anabaena variabilis were isolated by a method in which, after mutagenesis and before penicllin enrichment, mutant and wild-type cells were separated by cavitation. auxotrophs were identified by their inability to grow on minimal medium, and they were partially characterized by replica plating to media supplemented with single nutrients or specific groups of nutrients. of the 83 auxotrophs isolated, 65 required an inorganic source of nitrogen ...	1977	403180
temperature dependence on the delayed fluorescence of chlorophyll a in blue-green algae.	1. the delayed fluorescence of chlorophyll a was measured with a phosphoroscope by changing the temperature in a range of room temperatures in intact cells of blue-green algae, anacystis nidulans, two strains of anabaena variabilis and plectonema boryanum, and other kinds of algae, cyanidium caldarium and chlorella pyrenoidosa. the induction of delayed fluorescence remarkably depended on the temperature of measurment. nevertheless, the induction pattern was characterized by three levels of inten ...	1977	403943