Publications

guinea pig immunoglobulin light chain isotypes. i. separation of kappa and lambda chains and the identification of three isotypes of the lambda chain constant homology region.	the separation of intact kappa chain and two fragments comprising lambda (lambda) chain from immunoglobulin light chain pools isolated from strain 13 guinea pigs is achieved by cyanogen bromide digestion and gel filtration before and after reductive clevage of disulfide bonds. the smaller lambda chain fragment derives from the original carboxyl-terminus of lambda chain. the partial sequence of component tryptic and thermolytic peptides of this thirty-nine residue fragment allowed its complete se ...	1978	103729
transfer of 2,4,5,2',4',5'-hexachlorobiphenyl and 2,2-bis-(p-chlorophenyl),1,1,1-trichloroethane (p,p'-ddt) from maternal to newborn and suckling rats.	pregnant rats were given a small dose of 14c-2,4,5,2',4',5'-hexachlorobiphenyl (hcb) and 3h-ddt intraperitoneally. the transfer of hcb and ddt through the placenta and milk was then investigated. transfer through the placenta was 2.7 and 1.5% (respectively) of the initial doses; transfer through milk was 39.2 and 21.5%. hcb is obviously more transferable than ddt through the placenta and milk, the ratio of the amount of hcb transferred through milk to the amount transferred through the placenta ...	1978	104695
human bone marrow colony formation in diffusion chambers. normal values.		1978	340240
nucleotide sequence of the ampicillin resistance gene of escherichia coli plasmid pbr322.	i have determined the nucleotide sequence of the ampicillin resistance gene of pbr322, an escherichia coli plasmid that encodes a penicillin beta-lactamase. this gene codes for a protein of 286 amino acid residues. the first 23 amino acids presumably form a signal for secretion, because they do not appear in the mature enzyme, whose partial amino acid sequence has been determined independently.	1978	358200
retrospective study of an ehrlichia canis epizootic around phoenix, arizona.	antibodies to ehrlichia canis were detected by indirect immunofluorescence in sera from 40 of 339 dogs (12%) examined from around phoenix, az, during 1975. the prevalence was higher in purebred and crossbred german shepherd dogs (21%), as compared with 8% in the remaining breeds. the difference in antibody prevalence between sexes was not significant. dogs 12 weeks to 13 years old were seropositive. the causative agent was isolated by inoculation of whole blood from a seropositive dog into a sus ...	1978	624663
cyclic thrombocytopenia induced by a rickettsia-like agent in dogs.	hematologic manifestations and the ultrastructure of a platelet-specific microorganism isolated from a dog in florida were studied. the agent was readily transmitted experimentally to adult dogs by intravenous inoculation with infected blood. parasitemias and concomitant thrombocytopenias were cyclic in that both recurred within relatively constant periods of one to two weeks following experimental infections. hemorrhage was not a manifestation of the disease even though thrombocytopenias were s ...	1978	627738
effect of canine immune serum on the growth of ehrlichia canis within nonimmune canine macrophages.	the effect of canine immune serum (cis) on the growth of ehrlichia canis was studied in macrophage cultures derived from peripheral blood monocytes of normal dogs. ehrlichiae treated with canine normal serum and then introduced into normal macrophage cultures maintained in canine normal serum multiplied within the macrophages and destroyed them. immune serum, collected from e canis carrier dogs, suppressed ehrlichial growth in normal macrophages. an inverse relationship existed between the amoun ...	1978	629451
effect of canine immune macrophages and canine immune serum on the growth of ehrlichia canis.	the effect of canine immune macrophages and canine immune serum (cis) on the growth of ehrlichia canis was studied in vitro. treatment of e canis organisms with cis before they were inoculated into immune macrophage cultures, coupled with pre- and postinoculation maintenance of cultures in cis, suppressed macrophage infection rates to 1% or less. immune macrophages collected from e canis-carrier dogs and maintained in canine normal serum were more resistant to the intracellular growth of e canis ...	1978	629452
prophylactic and therapeutic use of tetracycline during an epizootic of ehrlichiosis among military dogs.	an epizootic of canine ehrlichiosis at the military working dog center, pakchong, thailand, was arrested by prophylactic and therapeutic use of tetracycline and by control of ticks. oral treatment with tetracycline (66 mg/kg of body weight daily for 14 days) effected remission of signs of disease in 60 of 62 clinically affected dogs and resulted in disappearance of serum antibody in 119 (60%) of 198 seropositive dogs treated during the 2-year study period. transmission of ehrlichia canis was int ...	1978	640933
[morphological changes in the digestive organs during prolonged space flight on the kosmos-782 biosatellite].	a reduction in the content of neutral mucopolysaccharides in mucous cells of the neck, a slight decrease in the activity of succinate dehydrogenase and nad-diaphorase in parietal cells, a decrease in the dna synthesis rate, and an increase in the area of mitochondria and cristae were detected in the gastric mucosa of rats which were in a long-term space flight. in the small intestine, an increase in the activity of glucose-6-phosphate dehydrogenase and leucine aminopeptidase were found. morpholo ...	1978	152100
specific labelling of replicating spp1 dna: analysis of viral dna synthesis and identification of phage dna-genes.	specific labelling of replicating bacteriophage spp1 dna can be achieved by infection at nonpermissive temperature of a b. subtilis strain carrying the initation mutation dnab ts134. under these conditions host dna synthesis is reduced by 90 to 95%. this technique was used to identify cistrons of spp1 involved in phage dna synthesis and to define intermediates in spp1 replication.	1978	105242
the measurement of bile acid-dependent and independent fractions of rat bile.	bile flow and bile acid secretory rate in rate in rats with interruption of the enterohepatic circulation had a biphasic decay with the second slower slope less marked for bile flow. a direct relationship between both parameters was observed in these animals. by knowing the choleretic efficiency of bile acids calculated from the regression line, the total bile acid output and the volume of bile collected, the fraction of bile produced by other way than bile water carried by bile acids was estima ...	1978	263162
a model of biliary pancreatic reflux.	a primate model for the study of biliary pancreatic reflux under relatively physiological conditions is described. cannulas were inserted into the gallbladder and the common bile duct of rhesus monkeys, and a pedicled segment of small bowel was used to create a pancreaticocutaneous fistula after resection of the spleen and pancreatic tail. following recovery, hypaque was instilled into the gallbladder with maintenance of common duct pressure within a normal range. the pancreatic duct was visuali ...	1979	105415
interference of iodine-125 ligands in radioimmunoassay: evidence implicating thyroxine-binding globulin.	although there is abundant published evidence that radioiodinated antigens interfere in digoxin radioimmunoassays, other radioimmunoassays are similarly affected. we investigated the relationship of radioiodinated antigen structure to its function in the immunoassay. carrier-free 125i-labeled iodotyrosine and iodohistamine derivatives were incubated with human serum, and the bound and free fractions were separated. we demonstrated that diiodotyrosyl analogs bind avidly to serum proteins. because ...	1979	108034
[intravenous cholangiography in hyperbilirubinaemia (author's transl)].	the effect of bilirubin on biliary and renal excretion of methylglucamine-iodipamide at an infusion rate of 1.3, 2.6 and 5.2 mu mol/min/kg was studied by intravenous injection into five dogs with normal liver functions and chronic duodenal fistulas. unconjugated bilirubin administered in a dose of 0.1 mu mol/min/kg reduced biliary iodipamide excretion and concentration significantly, but did not affect renal contrast material excretion. this indicates that the unsatisficatory radiological result ...	1979	153309
quantitative estimation of leishmanial antibody titers by enzyme-linked immunosorbent assay.	the response to immunization against leishmania braziliensis in rabbits was followed by measurement of antibody by enzyme-linked immunosorbent assay (elisa), passive hemagglutination, complement fixation, and countercurrent immunoelectrophoresis. passive hemagglutination and complement-fixation titers were interpreted according to standard methods, whereas elisa exact titers were derived by the equation y = a-b log x, which describes the straight line that results when absorbances are plotted ag ...	1979	387886
[dynamics of the development of different microorganisms in soil].	the dynamics of individual components from the complex of soil microorganisms (bacteria, fungi, actinomycetes) and the population of stm. olivocinereus introduced into soil was studied by luminescent microscopy and inoculation. the population density maxima for individual components from the complex of soil microorganisms were shown to be separated in time suggesting a succession. fungi developed at the first steps of succession and dominated in biomass over the remaining components of the compl ...	1979	470632
ehrlichiosis in wolves, dogs, and wolf-dog crosses.	canine ehrlichiosis was diagnosed in wolves, dogs, and wolf-dog crosses at a small zoo in northcentral florida. five of 9 adult canids and all 8 pups confined to a common kennel died as a result of the infection. the epizootic was associated with a massive rhipicephalus sanguineus infestation. hematologic and pathologic findings in an adult wolf-dog cross that died were characteristic of canine ehrlichiosis. that animal and four other canids were seropositive for ehrlichia canis. no deaths occur ...	1979	521367
specificity and biological distribution of coenzyme m (2-mercaptoethanesulfonic acid).	the specificity of the growth requirement of methanobacterium ruminantium strain m1 for a new coenzyme, 2-mercaptoethanesulfonic acid (hs--com), was examined. a variety of derivatives, analogs, and potential biosynthetic precursors of coenzyme m were tested; only a restricted range of thioether, thioester, and thiocarbonate derivatives of the cofactor were found to replace the hs--com requirement. bromoethanesulfonic acid (brch2ch2so3-), a halogenated analog of hs--com, potently inhibited the gr ...	1979	104960
influence of adrenocorticotropin on transport of a cholesteryl linoleate-low density lipoprotein complex into adrenal tumor cells.	the action of adrenocorticotropin (acth) on the specific (receptor-mediated) uptake of cholesteryl linoleate . low density lipoprotein complexes was examined in y-1 mouse adrenal tumor cells. high affinity binding (ka 4.1 x 10(8) m) was observed with acth; lower affinity was seen in the absence of acth. the effect of acth was observed within 10 min at physiological concentrations of low density lipoprotein (100 microgram/ml). binding was followed by uptake (internalization) of the ester . lipopr ...	1979	227899
a slowly dissociating form of the cell surface cyclic adenosine 3':5'-monophosphate receptor of dictyostelium discoideum.	experiments using a phosphodiesterase-minus mutant of dictyostelium discoideum indicate that ligand-induced loss of cell surface cyclic adenosine 3':5'-monophosphate binding sites (down regulation) can be evoked with concentrations of cyclic adenosine 3':5'-monophosphate as low as 10(-8) m. the loss of receptor sites is observed after 5 min of cell preincubation with cyclic adenosine 3':5'-monophosphate and can be as extensive as 75 to 80%. this decrease in binding sites is correlated with the a ...	1979	227901
marek's disease lymphoma of chicken and its causative herpesvirus.		1979	228727
isolation and partial biochemical characterization of the brush border plasma membrane from the cestode, hymenolepis diminuta.		1979	229204
effects of althesin and its steroidal components on a variety of excised smooth muscle preparations.		1979	262472
influence of the substituent radicals in antimonyl naphthalein derivatives on the potentialities of anti-schistosomal activity in mice.		1979	262474
effect of quantity and quality of igg antibodies on blocking of allergenic histamine release in vitro.	in a study of the protective role of igg antibodies in allergen injection therapy, serum antibodies to timothy grass allergens were raised in rabbits and tested for inhibition of histamine release from basophils of grass pollen-sensitive human subjects. rabbit sera were not inhibitory until late in the immune response when large amounts of relatively low affinity igg antibodies were detected by primary binding. inhibition of histamine release by late sera required preincubation of allergen and a ...	1979	84798
fatal adenovirus infection with misleading positive serology for infectious mononucleosis.	symptoms and serological tests in a 19-year-old man with pneumonia indicated that he had infectious mononucleosis. adenovirus type 7 was demonstrated in the lung by electron microscopy and was cultured from the liver, lung, lymph-nodes, and kidney.	1979	84950
safety of hepatitis-b vaccines containing intact 20 nm particles.		1979	85121
expression in escherichia coli of chemically synthesized genes for human insulin.	synthetic genes for human insulin a and b chains were cloned separately in plasmid pbr322. the cloned synthetic genes were then fused to an escherichia coli beta-galactosidase gene to provide efficient transcription and translation and a stable precursor protein. the insulin peptides were cleaved from beta-galactosidase, detected by radioimmunoassay, and purified. complete purification of the a chain and partial purification of the b chain were achieved. these products were mixed, reduced, and r ...	1979	85300
[mechanisms of the potentiating and blocking effects of the sera from tumor patients in the leukocyte migration inhibition reaction model].	in the model of the reaction of human blood leucocyte migration inhibition the in vitro study was conducted on some mechanisms of blocking and enhancement effects of the serum in 54 tumor patients. both effects were shown to be specific to an antigen. the enhancement effect could render a nonspecific action on intact cells in the presence of the corresponding antigen. this effect is thermolabile and may occur due to the interaction with cells. the blocking effect is thermostabile and may be real ...	1979	85367
the patterns and distribution of euchrysine-binding grains in nonstimulated human peripheral t and non-t lymphocytes.		1979	86386
effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to rat hypophyseal and hypothalamic cytosol macromolecules.	the binding of 5alpha-dihydrotestosterone to the hypophyseal and hypothalamic cytosol macromolecules prepared from castrated male rats was observed. the effects of antiprostatic agents on 5alpha-dihydrotestosterone binding to both hypophyseal and hypothalamic cytosol macromolecules was examined. cyproterone acetate and chlormadionone acetate showed the significant inhibiting effects on 5alpha-dihydrotestosterone binding to 7-8 s macromolecules of cytosol from both hypophysis and hypothalamus. sc ...	1979	86441
treatment of mammalian ova for the demonstration of nucleoprotein structures.		1979	86485
specificity of human lymphocytes primed against allogeneic cells in vitro. ii. discrimination and cross-reactivity in repeated priming.	when studying the specificity of human lymphocytes primed in vitro against hla-d determinants on allogeneic cells, it was found that specific restimulation of the primed cells 10 days after the first priming did not influence their discriminatory power compared to cells primed only once. likewise, priming to one hla-d determinant and repeated priming against another hla-d determinant did not change the discrimination for the first priming antigen. neither was there any increased relative reactiv ...	1979	87030
binding studies with antibodies having phosphorylcholine specificity and fragments derived from their homologous streptococcus pneumoniae type 27 capsular polysaccharide.	the capsular polysaccharide from streptococcus pneumoniae type 27 (s-27) has been fragmented into oligosaccharides by controlled degradations. binding of these derived small ligands with heterogeneous rabbit anti-s-27 antibody of selected phosphorylcholine affinity indicates that a major immunodeterminant of s-27 is the phosphorylcholine moiety, but that these anti-pc. antibodies are also capable of binding to the polysaccharide backbone from which the p.c. has been removed.	1979	87460
guinea pig transfer factor: restriction of macrophage migration inhibitory activity to extraordinary dilution confirmed with demonstration of diminished potency of stored preparations.		1979	88292
detection of adenovirus by enzyme-linked immunosorbent assay.	a solid-phase direct enzyme-linked immunosorbent assay (elisa) was developed for the detection of adenovirus antigen in extracts of infected cells by using antihexon serum. results with simulated clinical specimens consisting of normal nasal wash specimens seeded with varying concentrations of adenovirus type 5 showed that antigen could be detected in extracts of hep-2 cell cultures inoculated with 10(2.5) 50% tissue culture infective doses (tcid50) and 10(1.5) tcid50 after 2 and 4 days of incub ...	1979	88457
characterization of functional fc-receptor material from human lymphoblastoid cell lines. ii. serologic and cellular analysis.		1979	88475
studies on mononuclear phagocyte progenitor cells: morphology of cells and colonies in liquid culture of mouse bone marrow.		1979	88518
a technique for investigating the antigenicity of cultured rheumatoid synovial cells.	antisera to rheumatoid and nonrheumatoid synovial cells were prepared by: intraarticular injection of rabbits with these cells, subsequent synovectomy, and autologous immunization of each animal with the cultured rabbit synovial cells, or homogenized whole rabbit synovial membrane. resultant antisera were studied by immunofluorescence and 51chromium cytotoxicity tests against other cultured rheumatoid and nonrheumatoid cells. activity of positive sera could be removed by absorption with nonrheum ...	1979	88519
use of antibodies for studying the sidedness of membrane components.		1979	88659
[mrna of mouse plasmacytoma. reverse transcription and translation in cell-free systems].	total poly(a)-mrna from polyribosomes of mopc 21 mouse myeloma were investigated. poly(a)-mrna was released by two successive chromatography on oligo (dt)-cellulose. a 14s fraction of total poly(a)-mrna was obtained and partially purified by sucrose gradient centrifigation followed by acrylamide gel electrophoresis. as estimated from the electrophoretic analysis, the 14s mrna has three components, one of which appears to be 18s rrna and two others--mrnas with molecular weight of 5.2.10(5) and 3. ...	1979	88667
the influenza virus: antigenic composition and immune response.	the architecture and chemical composition of the influenza virus particle is described with particular reference to the protein constituents and their genetic control. the dominant role in infection of the surface proteins - haemagglutinins and neuraminidases - acting as antigens and undergoing variation in time known as antigenic drift and shift is explained. the immuno-diffusion technique has illuminated the interrelationships of the haemagglutinins of influenza a viruses recovered over long p ...	1979	88726
inhibition of spontaneous transformation of rat embryo cells releasing endogenous type c virus by virus-specific antiserum.	noninbred sprague-dawley rat embryo cell clones predictably undergo transformation after 20-30 in vitro passages following spontaneous release of endogenous rat leukemia virus (ralv). in the presence of ralv-specific antiserum, virus production and infectivity were reduced and transformation was delayed from 6 to 25 weeks. transformation was not associated with an increased expression of kirsten murine sarcoma virus-related src gene rna.	1979	89211
amylase levels in the tissues and body fluids of the domestic cat (felis catus).	1. the amylase activities of serum, liver, pancreas, submaxillary and parotid glands, saliva, duodenum, colon, lung, heart, spleen, kidney and skeletal muscle of the domestic cat, were determined. 2. as in most mammals, the highest amylase content was found in the pancreas. levels in saliva and salivary glands were very low. 3. amylase activities of the serum, liver and pancreas have an optimum ph 7.0-7.5. 4. amylases of serum, liver and pancreas were inactivated by removal of chloride and these ...	1979	95689
positive ehrlichia canis serology in kawasaki disease.		1980	6103489
somatic cell hybrids of canine peritoneal macrophages and sv40-transformed human cells: derivation, characterization, and infection with ehrlichia canis.	somatic cell hybrids were obtained by fusion of canine peritoneal macrophages and sv40-transformed human skin fibroblasts. a cell line (wrh-2) was established from a single isolated hybrid clone. the wrh-2 cell line has been serially passaged 60 times and has a population doubling time of approximately 24 hours. karyotypic analysis showed the modal number of chromosomes to be 80, with a selective segregation of canine chromosomes. expression of incorporated canine dna was substantiated by cellul ...	1980	6245605
cell-mediated and humoral immune responses of german shepherd dogs and beagles to experimental infection with ehrlichia canis.	the cell-mediated and the humoral immune responses of 12 german shepherd dogs and 5 beagles inoculated with ehrlichia canis were evaluated. results indicated that specific and nonspecific immunosuppression due to e canis occurred in the german shepherd dogs. canine leukocyte migration-inhibition factor was successfully isolated and shown to be physically and functionally similar to human and guinea pig migration inhibition factor. of the german shepherd dogs, 58% developed positive cell-mediated ...	1980	6989300
serial propagation of ehrlichia canis in primary canine peripheral blood monocyte cultures.	cultures of primary canine monocytes were prepared from blood to which dextran (3%) had been added to enhance sedimentation of the erythrocytes. homogeneous populations of monocytes were observed by 5 days after seeding, and some cultures were maintained as long as 30 days. the monocyte cultures were used successfully for the serial passage of ehrlichia canis. after the 3rd passage, small intracytoplasmic inclusions of e canis were detected by 60 hrs after inoculation and greater than 90% of the ...	1980	7379552
serum complement and blood platelet adhesiveness in acute canine ehrlichiosis.	three dogs inoculated with ehrlichia canis served as infected controls (group i), 3 dogs treated with cobra venom factor (cvf) after inoculation with e canis were the principals (group ii), 3 noninfected dogs were the noninfected controls (group iii), and 1 noninfected dog given cvf was the noninfected, decomplemented control (group iv). thrombocytopenia occurred in all e. canis-inoculated dogs (groups i and ii) within 3 weeks after they were inoculated. however, the severity of the thrombocytop ...	1980	7447122
free-living jackals (canis mesomelas)-potential reservoir hosts for ehrlichia canis in kenya.	using a modified cell culture test, ehrlichia canis was found in eight of 15 free-living jackals (canis mesomelas) and 14 of 31 dogs owned by pastoral communities in the same areas of kenya. two cross-bred puppies inoculated with blood from infected jackals developed mild, transient clinical disease, and e. canis was recovered from the puppies. tick species found on the jackals were similar to those found on the infected dogs. ehrlichia canis was not found in eight spotted hyaenas (crocuta crocu ...	1980	7463598
acute experimental canine ehrlichiosis. i. sequential reaction of the hemic and lymphoreticular systems.	dogs with acute ehrlichia canis infection showed the established clinical features of acute ehrlichiosis and had thrombocytopenia, anemia, increased serum glutamic pyruvic transaminase activity, and decreased total serum protein and albumin concentrations during the first weeks after infection, and increasing gamma globulin concentrations after the third week. gross lesions in hemic and lymphoreticular tissues were transient lymphadenomegaly, splenomegaly, and increased red long-bone marrow. lym ...	1981	7467072
evidence of a serologic relationship between ehrlichia canis and rickettsia sennetsu.	a serologic relationship between rickettsia sennetsu, the etiologic agent of human sennetsu rickettsiosis in western japan, and ehrlichia canis, the agent of canine ehrlichiosis, has been demonstrated. using the indirect fluorescent antibody test, convalescent sera from patients with sennetsu fever reacted with ehrlichia canis antigen. in the direct fluorescent antibody test, immunoglobulins from four patients with sennetsu rickettsiosis stained e. canis morulae in canine monocytes. this finding ...	1981	7034563
acute experimental canine ehrlichiosis. ii. sequential reaction of the hemic and lymphoreticular system of selectively immunosuppressed dogs.	the lymphoreticular response of dogs to ehrlichia canis infection was studied after immunosuppressive therapy with cyclophosphamide or antilymphocyte serum. immunosuppression did not increase mortality, nor did it prevent or significantly modify the clinical manifestations of acute ehrlichiosis. the clinical, hematologic and serum biochemical characteristics of acute ehrlichiosis appeared seven or more days later in immunosuppressed than in immunologically intact infected dogs. immunosuppressive ...	1981	7257081
attempted transmission of canine ehrlichiosis to the vervet monkey (cercopithecus pygerythrus).	blood from a domestic dog infected with ehrlichia canis was injected into 3 adult vervet monkeys cercopithecus pygerythrus. the monkeys did not develop any clinical or haematological signs of disease, nor did they seem to be able to harbour the parasite. furthermore, neither morulae of e. canis nor haematological evidence of ehrlichiosis were seen after these monkeys had been splenectomized. the splenectomized monkeys did, however, develop a severe parasitaemia of their red cells with entopolypo ...	1981	7312306
distribution of ehrlichia canis among military working dogs in the world and selected civilian dogs in the united states.	antibodies to ehrlichia canis were detected by indirect immunofluorescence in sera from 233 of 2,077 (11%) military working dogs in various locations throughout the world and from 535 of 938 (57%) civilian dogs in the united states during a 1-year period of study. overall, e canis infection rates ranged from 13% in the tropical and temperate zones below 45 degrees n to 8% in the cold zone north of 45 degrees n latitude. the highest antibody prevalence rate (24%) was found among a select populati ...	1982	7050062
rickettsia sennetsu in human blood monocyte cultures: similarities to the growth cycle of ehrlichia canis.	microscopic examination of cultured human monocytes infected with rickettsia sennetsu and stained by the giemsa method revealed the presence of various organismal growth forms in the cytoplasm of the infected cells. the growth forms were loosely scattered individual organisms, clusters of organisms, various sizes of dense inclusion bodies in intact and vacuolated cytoplasm, and organisms in close proximity to disintegrated monocytes. the appearance and the morphology of these r. sennetsu growth ...	1982	6274798
serologic diagnosis of infectious cyclic thrombocytopenia in dogs using an indirect fluorescent antibody test.	an indirect fluorescent antibody test was used for detection of serum antibodies to the platelet-specific rickettsial organism that is the causative agent of infectious cyclic thrombocytopenia (ict) in dogs. the test converted from negative to positive in 7 of 7 experimentally inoculated dogs. one of 2 attempts to recover the rickettsial agent of ict from naturally occurring seropositive dogs, by blood inoculation of experimental dogs, was successful. seemingly, the test did not detect antibodie ...	1983	6362502
treatment and thrombocyte levels in experimentally induced canine ehrlichiosis and canine babesiosis.	three dogs which were carriers of babesia canis were infected with ehrlichia canis. these animals developed clinical signs and haematological evidence of ehrlichiosis and made an uneventful recovery, despite the fact that they were subsequently treated with doxycycline only. three control dogs which were also carriers of b. canis were clinically normal, despite the fact that they showed a distinct drop in the thrombocyte levels associated with increases in the numbers of parasitized red cells.	1983	6676688
serum hyperviscosity syndrome associated with ehrlichia canis infection in a dog.	a 10-year-old male lhasa apso was examined because of pyrexia, vomiting, weight loss, lethargy, and disorientation. the clinical and laboratory findings were supportive of a diagnosis of serum hyperviscosity syndrome associated with an ehrlichia canis infection. after tetracycline therapy the serum hyperviscosity syndrome and e. canis infection were successfully resolved.	1983	12002576
acrylamide effects on the macaque visual system. i. psychophysics and electrophysiology.	oral acrylamide produces axonal swelling and later degeneration and gliosis in the distal optic tract, especially within the lateral geniculate nucleus, of macaque monkeys. measures of visual thresholds and cortical-evoked potentials were used to study the time course of visual changes during exposure to acrylamide in macaque monkeys. contrast sensitivity, visual acuity, and flicker fusion frequency were reduced during exposure, and only flicker fusion recovered rapidly and completely after expo ...	1985	3972511
clinical and hematologic findings in canine ehrlichiosis.	the clinical and hematologic findings for 56 dogs with ehrlichiosis were studied retrospectively. all dogs had a high serum antibody titer to ehrlichia canis. the frequency of bleeding disorders, thrombocytopenia, and pancytopenia in these dogs was lower than previously described for dogs so affected. a bleeding disorder caused by a suspected qualitative platelet defect was found in some dogs that did not have thrombocytopenia. megakaryocytic hyperplasia and high numbers of plasma cells were obs ...	1985	3972694
rocky mountain spotted fever in dogs and its differentiation from canine ehrlichiosis.	rocky mountain spotted fever (rmsf) or ehrlichiosis was diagnosed in dogs on the basis of specific immunofluorescent testing for each disease. comparisons between clinical and laboratory findings were made between the 2 diseases. the incidence of rmsf tended to be more seasonal and it affected younger dogs. purebred dogs appeared to be more susceptible to both diseases. in general, rmsf had a more rapid and severe course of clinical illness than did ehrlichiosis, but acute ehrlichiosis was diffi ...	1985	3972706
illness associated with campylobacter laridis, a newly recognized campylobacter species.	campylobacter laridis, a recently described thermophilic campylobacter species found principally in seagulls, has not previously been linked to illness in humans. six clinical isolates of this species were referred to the national campylobacter reference laboratory in 1982 and 1983. each isolate was confirmed by biochemical characterization and by dna relatedness studies. the six isolates were obtained during an illness: enteritis in four, severe crampy abdominal pain in one, and terminal bacter ...	1985	3972989
activation of branched-chain alpha-ketoacid dehydrogenase complex by alpha-chloroisocaproate in normal and enzyme-deficient fibroblasts.	a method has been developed for the activation of the branched-chain alpha-ketoacid dehydrogenase complex by alpha-chloroisocaproate, an inhibitor of branched-chain alpha-ketoacid dehydrogenase kinase in human cultured skin fibroblasts. the enzyme could be activated by pretreating the cells with alpha-chloroisocaproate before they were disrupted for measurement of the activity. after this treatment, the activity was 2- to 3-fold that of untreated cells (24.8-81.4 pmol/min per mg protein). the en ...	1985	3987057
peritubular protein modulates neutral active nacl absorption in rabbit proximal convoluted tubule.	to assess directly the effect of increasing and decreasing peritubular protein concentration on active nacl absorption, rabbit proximal convoluted tubules were perfused with a high-chloride solution simulating late proximal tubular fluid and bathed in a similar solution containing 0, 6, or 10 g/dl albumin. a direct examination of the active component of nacl absorption was ensured by 1) the reduction of luminal solutes other than nacl (no glucose or alanine and only 5 mm hco-3), and 2) the use o ...	1985	4003554
continuous propagation of ehrlichia sennetsu in murine macrophage cell cultures.	ehrlichia sennetsu, the etiologic agent of human sennetsu rickettsiosis was successfully propagated in a continuous cell culture using murine cell lines p388d1 and raw 264. pleomorphic cytoplasmic inclusion bodies similar to ehrlichia canis morulae were observed 3-4 days after second post-inoculation split. in the raw 264 cell line e. sennetsu was not seen until the third passage. relatively heavier infection was observed in p388d1 than in raw cell line. the latter reached a maximum of 15% infec ...	1985	4025693
does the in-utero exposure to furosemide delay the renal maturation?	two groups of pregnant wistar rats were constituted, one treated with furosemide ip and one with saline. the drug was given on days 7-11 and 14-18 of the pregnancy. litters from the two groups were not different in number of pups, body weight, and/or kidney weight, but exposed in-utero neonates, studied shortly after birth, exhibited a significant lower number of differentiated glomeruli than those of the control group. as well as the difficulty of explaining this phenomenon, this condition rais ...	1985	4047752
microwaves and the cell membrane. ii. temperature, plasma, and oxygen mediate microwave-induced membrane permeability in the erythrocyte.	microwaves (2450 mhz) are shown to increase 22na permeability of rabbit erythrocytes for exposures only within the narrow temperature range of 17.7 to 19.5 degrees c (tc) which coincides with a nonlinearity in the arrhenius plot reflecting an apparent membrane phase transition. significantly, this response is not observed for cholesterol-loaded erythrocyte membranes which exhibit a linear arrhenius plot and no apparent phase transition at tc. the permeability increase at tc is a nonlinear functi ...	1985	4070542
[the effect of large dose dexamethasone on local cerebral blood flow (l-cbf) in ischemic rat brain].	changes of local cerebral blood flow (l-cbf) were estimated by autoradiographic techniques in middle cerebral artery occlusion rats given a large dose of (3 mg/kg) dexamethasone. the sensory-motor, parietal, auditory and olfactory cortices showed a significant reduction (greater than 25%) of l-cbf. in mca occlusion with large dose dexamethasone group, ischemic cortices showed significant reduction from the only mca occlusion group (highest 36%). there was no significant reduction of l-cbf in the ...	1985	4074585
experimental and clinical trials of long acting oxytetracycline in the treatment of canine ehrlichiosis.	four splenectomized dogs were experimentally infected with ehrlichia canis and treated at the point of illness with long acting (la) oxytetracycline at different dosages. terramycin la, when given at a dose of 20 mg/kg body weight (deep intramuscularly) twice, at a four-day interval, was found to have effectively controlled the disease and has replaced the usual 7-14 successive days' treatment regimen when other groups of tetracycline drugs are used. predef 2x at the rate of 2 mg given intramusc ...	1985	3892880
new model systems to study dna-protein recognition mechanisms.	dpg antibodies were fractionated on a cellulose-double-stranded dna column. the flow-through fraction bound denatured dna but not double-stranded dna (dsdna). the dpg-eluted fraction bound dsdna preferentially. the results show that proteins can recognise dpg in dna by different mechanisms, some involving dna unwinding.	1985	3896854
protection by candida albicans of staphylococcus aureus in the establishment of dual infection in mice.	candida albicans has been shown to stimulate infection in mice by a number of bacteria when both organisms are inoculated intraperitoneally (e. carlson, infect. immun. 39:193-197, 1983). when subcutaneous and intraperitoneal inoculations were given with staphylococcus aureus and c. albicans injected at opposite sites, mixed infection was established at the site of fungal inoculation but not at the site of the bacterial injection. histopathologic evaluation of tissues for the presence of c. albic ...	1985	3905609
protection by candida albicans of staphylococcus aureus in the establishment of dual infection in mice.	candida albicans has been shown to stimulate infection in mice by a number of bacteria when both organisms are inoculated intraperitoneally (e. carlson, infect. immun. 39:193-197, 1983). when subcutaneous and intraperitoneal inoculations were given with staphylococcus aureus and c. albicans injected at opposite sites, mixed infection was established at the site of fungal inoculation but not at the site of the bacterial injection. histopathologic evaluation of tissues for the presence of c. albic ...	1985	3905609
charge recombination kinetics as a probe of protonation of the primary acceptor in photosynthetic reaction centers.	the kinetics of the charge recombination d+qa-----dqa was used to probe the protonation of the primary acceptor in reaction centers from rhodopseudomonas sphaeroides, in which the native ubiquinone was replaced by anthraquinone. we found that qa- is stabilized by the rapid (t less than 10(-2) s) binding of a proton, with a pk of 9.8. the distance between qa- and the proton binding site was estimated to be larger than approximately 5 a.	1985	3907729
glutaminyl-trna synthetase of escherichia coli.		1985	3911010
the incidence of canine haematozoa in peninsular malaysia.	in 3 urban areas in selangor, peninsular malaysia between 1973 and 1981, blood from 4084 dogs was examined for haematozoa. the following frequencies were found: babesia gibsoni 17.7%; microfilariae of dirofilaria immitis 9.6%; hepatozoon canis 1.2%; b. canis 1.1%; ehrlichia canis 0.2%; trypanosoma evansi 0.1%. a detailed examination of b. gibsoni infections and microfilariasis due to d. immitis with regards to monthly distribution, breed frequency, sex and age, revealed that pedigree and non-ped ...	1985	3922103
sensitivity of phospholipase c (bacillus cereus) activity to phosphatidylcholine structural modifications.	the structural features of a phosphatidylcholine molecule important for binding to phospholipase c (bacillus cereus) have been examined using kinetic analyses of a series of short-chain phosphatidylcholines and analogues. lipids examined had varying chain lengths, methyl branched chains, phenyl alkanoate chains, and a single fatty acyl chain (lysophosphatidylcholines). a comparison of vmax and km for monomolecularly dispersed dibutyroyl-, dihexanoyl- and diheptanoylphosphatidylcholine indicates ...	1985	3933566
adaptation of ehrlichia sennetsu to canine blood monocytes: preliminary structural and serological studies with cell culture-derived ehrlichia sennetsu.	ehrlichia sennetsu, the causative agent of human sennetsu rickettsiosis, was successfully propagated in primary canine blood monocyte cultures. the growth cycle of this organism appears to be similar to that of ehrlichia canis. the antigen derived from our e. sennetsu cultures was used to develop an indirect fluorescent antibody test for detection and titration of serum antibodies to the organism. using this test system, we found that five human serum samples obtained from patients clinically di ...	1985	2985504
binding characteristics of swine erythrocyte insulin receptors.	crossbred gilts (controls; n = 7) had 8.8 +/- 1.1% (mean +/- sem) maximum binding of [125i]insulin to insulin receptors on erythrocytes. the number of insulin-binding sites per cell was 137 +/- 19, with a binding affinity ranging from 7.4 x 10(7)m-1 to 11.2 x 10(7)m-1 and mean of 8.8 x 10(7)m-1. pregnant sows (n = 5) had a significant increase (p less than 0.01) in maximum binding due to an increase in number of receptor sites per cell. lactating sows fed a high-fiber diet (n = 3) and a low-fibe ...	1985	2996389
the role of structure in the disposition of halogenated aromatic xenobiotics.	halogenated aromatic xenobiotics such as the chlorinated and brominated biphenyls, naphthalenes, dibenzodioxins, and dibenzofurans are widespread environmental contaminants. the number, position, and nature of the halogen atoms as well as the structure of the aromatic rings influence the disposition of these chemicals in living systems. absorption is governed primarily by the physical properties of lipophilicity and solubility. distribution through the blood occurs by nonspecific binding to plas ...	1985	2998745
prevalence of cytomegalovirus antibodies in thai blood donors.	antibodies to cytomegalovirus (cmv) were determined in thai blood donors using the complement fixation (cf) test and enzyme-linked immunosorbent assay (elisa). a total of 203 voluntary blood donors, 181 males and 22 females, who came to the blood bank at siriraj hospital during february 1985, were investigated. their ages ranged from 17 to 53 years (mean 24.3 +/- 6.9). seventy-three out of 156 (46.8%) and 171 out of 203 (84.2%) sera were positive for cmv antibodies as detected by the cf test and ...	1985	3000401
the inhibition of progesterone secretion and the regulation of cyclic nucleotides by atrial natriuretic factor in gonadotropin responsive murine leydig tumor cells.	we have found that atrial natriuretic factor (anf) has a profound effect on testicular cells in altering intracellular cyclic nucleotide levels as well as progesterone secretion. using clonal cultured leydig tumor cells we found that 1 x 10(-8)m anf caused a two thousand-fold elevation in the accumulation of cellular cgmp and inhibited camp in treated cells by more than 90% as compared to the controls. anf (1 x 10(-8)m) also significantly inhibited gonadotropin-stimulated accumulation of camp in ...	1985	3002360
[cyclic amp- and atp-mediated stimulation of dna synthesis following partial hepatectomy by prostaglandin-e1 in d-galactosamine injured liver].	d-galactosamine (d-gal) damaged rats were infused with prostaglandin e1 (pge1) through a peripheral vein for 40 min. before and after partial hepatectomy. dna synthesis following 68% partial hepatectomy was severely inhibited by the pretreatment of d-galactosamine. pge1 infusion (0.5, 1.0 microgram/kg/min) enhanced the dna synthesis inhibited by d-gal 600 mg/kg significantly (p less than 0.01). after 20 min. of pge1 infusion cyclic amp levels of liver tissue was increased as compared with saline ...	1985	3003557
structural bases of the cytolytic mechanisms of entamoeba histolytica.	the cellular bases of the powerful cytolytic activity of the human protozoan parasite entamoeba histolytica were explored by studying the effect of the virulent strain hm1:imss on epithelial monolayers of mdck cells using a combination of time-lapse microcinematography and transmission and scanning electron microscopy. early alterations of the epithelial cell membranes were detected by measuring changes in the transepithelial electrical resistance of mdck monolayers mounted in ussing chambers. t ...	1985	2859368
[interaction of local anesthetics with secretory cells at the membrane level].		1985	2408715
the perivascular cell populations in human skin after topical application of leukotriene b4.	fifteen timed biopsies of human skin were performed after application of 100 nanograms of leukotriene b4 in a finnchamber for six hours. the number of inflammatory cells were counted per high power perivascular field and compared to three control biopsies. at 24 hours a peak of neutrophiles was observed and subsequently lymphocytes predominated. eosinophils were never prominent. this pattern of successive cell populations has been described in inflammatory and whealing skin disease.	1986	2430407
chronic stimulation modifies the isotonic shortening velocity of denervated rat slow-twitch muscle.	rat soleus muscles were denervated and stimulated in vivo for periods of up to 104 days. stimuli used were trains of 1 ms pulses at 100 hz delivered for periods of 1 s; trains were repeated every 10-100 s. in a majority of animals the tension of the muscles was maintained at about 10% of normal, equivalent to muscles denervated but unstimulated for 20 days. at the longest periods the stimulated muscles developed ten times more tension than ones that were denervated but not stimulated. in denerva ...	1986	2874561
human papillomavirus types 16 and 18 sequences in early cervical neoplasia.	a total of 100 colposcopic biopsies from patients with abnormal papanicolau's tests were surveyed for the presence of human papillomavirus (hpv) types 16 and 18 sequences by spot-blot hybridization. hpv 16 and 18 dna sequences were detected in 58% of the biopsies. none of the cervical intraepithelial neoplasia grade i (cin i) contained hpv 16 while 50% of the cin iii lesions (carcinoma in situ, cis) contained hpv 16. hpv 18-related sequences were equally represented in cin i, ii, and iii. southe ...	1986	3022464
downstream sequences affect transcription initiation from the adenovirus major late promoter.	we analyzed a set of adenovirus-simian virus 40 (sv40) hybrids in which the sv40 t antigen coding sequences are inserted downstream from the adenovirus major late promoter within the first, second, and third segments of the tripartite leader. in infected cells, these viruses give rise to a matched set of hybrid sv40 mrnas that differ only in the number of tripartite leader segments attached to the complete sv40 t antigen coding region. we found that the number of tripartite leader segments prese ...	1986	3023944
cloning and characterization of the high-affinity camp phosphodiesterase of saccharomyces cerevisiae.	a gene, pde2, has been cloned from the yeast saccharomyces cerevisiae that, when present in high copy, reverses the phenotypic effects of ras2val19, a mutant form of the ras2 gene that renders yeast cells sensitive to heat shock and starvation. it has previously been shown that the ras proteins are potent activators of yeast adenylate cyclase. we report here that pde2 encodes a high-affinity camp phosphodiesterase that shares sequence homology with animal cell phosphodiesterases. these results t ...	1986	3025832
challenging conventional aminoglycoside dosing regimens. the value of experimental models.	although newer antimicrobials look promising for the treatment of serious gram-negative infections, aminoglycosides still remain part of the mainstay of their therapy. traditional intermittent therapy is based upon the premise that high serum aminoglycoside concentrations are toxic. however, the rate of bacterial killing for aminoglycosides is also a concentration-dependent phenomenon. two animal models of pseudomonas pneumonia were utilized to examine the efficacy of non-traditional aminoglycos ...	1986	3089001
daily urinary protein loss in schistosoma haematobium infection.	proteinuria was studied in 128 children aged 6 to 18 years with schistosoma haematobium infection in the people's republic of congo. urinary protein concentration in spontaneously voided midday urine of patients with greater than 100 ova/10 ml was significantly higher than in 24-hr urine specimens. median daily urinary protein loss in patients with moderate intensity of infection (100-350 ova/10 ml) was 300 mg and 584 mg/1.73 m2 body surface in heavily infected patients (greater than 350 ova/10 ...	1986	3094395
enzyme histochemistry of human melanomas and pigmented naevi with special reference to alpha-d-mannosidase activity.	a histochemical study of alpha-d-mannosidase revealed that normal human melanocytes (resting state, activated, lentigo simplex) exhibit either no or just detectable activity, as do melanocytes in the initial phase of lentigo maligna. junctional, or occasionally zone a naevocytes displayed a very low enzyme activity. on the other hand, melanocytes in the initial stage of neoplastic transformation (dysplastic naevi, advanced stage of lentigo maligna) and also melanoma cells in disorders of low mal ...	1986	3096914
enzymatic cleavage of various fluorinated pyrimidine nucleosides to 5-fluorouracil and their antiproliferative activities in human and murine tumor cells.		1986	2951016
gastrin stimulates growth of colon cancer.	gastrin is trophic for normal gastric and colonic mucosa. we examined the potential trophic effects of chronic gastrin administration on the growth of mouse colon adenocarcinoma (mc-26). thirty-three mice bearing transplantable mc-26 colon cancers were treated with varying doses (125, 250, or 500 micrograms/kg/day) of pentagastrin. significant increases in tumor weight and dna content were observed. fundic mucosal weight and dna content in these mice showed a dose-related trophic response. the w ...	1986	3952654
ehrlichia canis-related polyarthritis in a dog.	ehrlichia canis-related polyarthritis was diagnosed in a 7-month-old boxer. the diagnosis was based on intraneutrophilic morulae found in synovial fluid specimens, thrombocytopenia, a positive result for indirect fluorescent antibody testing for e canis, the presence on the dog of the known vector of e canis infection (rhipicephalus sanguineus), and a favorable response to treatment with tetracycline hydrochloride. the dog has had no recurrence of lameness for 18 months after cessation of treatm ...	1986	3533872
multiplication-stimulating activity-induced alkalinization of canine renal proximal tubular cells.	the actions of a variety of polypeptide growth factors on isolated cells are thought to be initiated by stimulation of na+-h+ exchange across the plasma membranes of the cells resulting in intracellular alkalinization. to determine whether insulin-like growth factors (igfs) exert actions through such a mechanism, we incubated suspensions of canine renal proximal tubular segments with insulin or igf i or with multiplication-stimulating activity (msa)/igf ii. changes in intracellular ph were detec ...	1986	3533920
kinetic analysis of lamb mutants suggests the signal sequence plays multiple roles in protein export.	we have developed a quantitative assay to measure the rate of processing of precursor lamb into mature protein and have used this assay to characterize 10 previously isolated and 3 new lamb signal sequence mutants. the data suggest that the lamb signal sequence serves a complex function. our assay has revealed five types of signal sequence defect: 1) a strong kinetic defect resulting from alteration of the secondary structure in the putative alpha-helical region in the hydrophobic core, 2) a str ...	1986	3533933
biosynthetic preparation of radioactively labelled ethanolamine plasmalogen (1-o-[1'-14c]octadec-1'-enyl-2-acyl-sn-glycero-3-phosphoethanola mine) using a protozoan cell culture.	ethanolamine plasmalogen radiolabelled mainly in the o-alkenyl moiety was prepared from cell suspension cultures of the flagellate leishmania donovani previously incubated with [1-14c]octadecanol over one growth period. the optimal concentration of [1-14c]octadecanol for labelling was shown to be 1 microm, when 60% of total lipid radioactivity appeared in the 1,2-diradyl-sn-glycero-3-phosphoethanolamine fraction, with an overall yield of approx. 35%. analysis of this fraction revealed that 93% o ...	1986	3536139