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16s-23s rdna internal transcribed spacer sequences for analysis of the phylogenetic relationships among species of the genus fusobacterium.the 16s-23s rdna internal transcribed spacer (its) regions of all currently defined fusobacterium species and related taxa such as leptotrichia buccalis, sebaldella termitidis and streptobacillus moniliformans, were analysed to examine inter- and intraspecies as well as subspecies relationships. for the its-amplification, a new eubacterial universal primer pair was designed and used. the majority of the fusobacterium strains, along with l. buccalis showed one major, and two to three weaker, dist ...200211931161
update on the taxonomy and clinical aspects of the genus fusobacterium.the genus fusobacterium currently includes 13 species. fusobacterium nucleatum, the most frequently encountered species in humans, is heterogeneous and currently includes 5 subspecies. a potentially new subspecies of f. nucleatum that is intrinsically quinolone-resistant and phylogenetically separate from the other 5 subspecies has been identified from dog and cat oral flora. two subspecies have been described for fusobacterium necrophorum, and a new species, fusobacterium equinum, which is rela ...200212173104
in vitro activities of faropenem against 579 strains of anaerobic bacteria.the activity of faropenem, a new oral penem, was tested against 579 strains of anaerobic bacteria by using the nccls-approved reference method. drugs tested included amoxicillin-clavulanate, cefoxitin, clindamycin, faropenem, imipenem, and metronidazole. of the 176 strains of bacteroides fragilis group isolates tested, two isolates had faropenem mics of 64 micro g/ml and imipenem mics of >32 micro g/ml. faropenem had an mic of 16 micro g/ml for an additional isolate of b. fragilis; this strain w ...200212384389
a.f.p. in amniotic fluid and serum. 200277393
studies on the structure of the fragment d moiety of abnormal fibrinogens: influence of calcium ions. 2003154750
activities of garenoxacin (bms-284756) and other agents against anaerobic clinical isolates.a total of 590 clinical isolates consisting of 33 species of both gram-positive and gram-negative anaerobes were collected from nine centers in the chicago area in 1998-1999. the largest number of isolates (330 isolates, 56%) belonged to the bacteroides group. isolates were tested by agar dilution against garenoxacin (bms-284756, t-3811 me), trovafloxacin, moxifloxacin, clindamycin, imipenem, piperacillin-tazobactam, and cefoxitin. all but one species (2% of bacteroides vulgatus isolates) were f ...200312604521
multibacterial sepsis in an alcohol abuser with hepatic cirrhosis.an alcohol abuser with hepatitis c developed multibacterial sepsis. his mean 100% alcohol intake reached 400 ml/day. in january 2001, he suddenly experienced fever (39 degrees c) with no other symptoms. one week later, he was admitted to our hospital and was subsequently diagnosed with sepsis associated with four species of bacteria (streptococcus constellatus, fusobacterium mortiferum, bacteroides thetaiotaomicron, and non-spore-forming anaerobic gram-positive bacillus). a drip infusion of imip ...200312636245
usefulness of the microseq 500 16s ribosomal dna-based bacterial identification system for identification of clinically significant bacterial isolates with ambiguous biochemical profiles.due to the inadequate automation in the amplification and sequencing procedures, the use of 16s rrna gene sequence-based methods in clinical microbiology laboratories is largely limited to identification of strains that are difficult to identify by phenotypic methods. in this study, using conventional full-sequence 16s rrna gene sequencing as the "gold standard," we evaluated the usefulness of the microseq 500 16s ribosomal dna (rdna)-based bacterial identification system, which involves amplifi ...200312734240
in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, bacitracin, and four other antimicrobials against intestinal anaerobic bacteria.by using an agar dilution method, the in vitro activities of ramoplanin, teicoplanin, vancomycin, linezolid, and five other agents were determined against 300 gram-positive and 54 gram-negative strains of intestinal anaerobes. ramoplanin was active at <or=2 microg/ml against 287 of 300 (95.7%) gram-positive organisms, including 18 strains of clostridium difficile for which mics of ramoplanin were 0.25 to 0.5 microg/ml; for 3 of these, linezolid mics were 8 to 16 micro g/ml. nineteen clostridium ...200312821492
severe orthopedic disability in childhood: solutions provided by rehabilitation engineering. 2003149287
diagnostic accuracy of the electrocardiogram. 2003145318
a new device to support handicapped patients during venography or myelography.the authors describe a seating device which holds the patient in position when the x-ray table is tilted and permits non-weight-bearing venograms to be obtained in handicapped as well as normal patients. simultaneous bilateral venography reduces the study time considerably. the device is also helpful in lumbar myelography in paraplegics.2003149336
[70th birthday of dr. karel raska]. 2003161871
[how does one calculate the activity of a radiodiagnostic service?]. 2004529236
prognistic relevance of radiation induced immune suppression in breast carcinoma.the extent of radiation induced immune suppression was analysed in 100 patients with carcinoma of the breast. the relative changes of lymphocyte counts and stimulations by pha and ppd were similar in patients who differed with regard to age, size of tumour and its malignancy grade or axillary node condition. moreover, no difference in the degree of radiation induced immune suppression existed between patients who developed recurrent disease and those who remained free of disease during a follow- ...2004525447
prophylactic infusion therapy in hemophilia. 2004478502
fibrinolytic therapy for progressive systemic sclerosis and tissue fibrinolytic activity in affected skin. 2004353113
genes malh and pagl of clostridium acetobutylicum atcc 824 encode nad+- and mn2+-dependent phospho-alpha-glucosidase(s).the genome of clostridium acetobutylicum 824 contains two genes encoding nad+, mn2+, and dithiothreitol-dependent phospho-alpha-glucosidases that can be assigned to family 4 of the glycosylhydrolase superfamily. the two genes, designated malh (maltose 6-phosphate hydrolase) and pagl (phospho-alpha-glucosidase), respectively, reside in separate operons that also encode proteins of the phosphoenolpyruvate-dependent:sugar phosphotransferase system. c. acetobutylicum grows on a variety of alpha-link ...200414570887
identification and characterization of a novel adhesin unique to oral fusobacteria.fusobacterium nucleatum is a gram-negative anaerobe that is prevalent in periodontal disease and infections of different parts of the body. the organism has remarkable adherence properties, binding to partners ranging from eukaryotic and prokaryotic cells to extracellular macromolecules. understanding its adherence is important for understanding the pathogenesis of f. nucleatum. in this study, a novel adhesin, fada (fusobacterium adhesin a), was demonstrated to bind to the surface proteins of th ...200516030227
thyroid abscess due to a mixed anaerobic infection with fusobacterium mortiferum.a rare case of a thyroid abscess due to mixed anaerobic flora containing fusobacterium mortiferum in an immunocompetent patient is described. the patient was successfully treated with immediate surgical intervention and appropriate antimicrobial agents.200516333131
in vitro activity of linezolid against clinical isolates of fusobacterium spp.although most susceptibility studies for linezolid have investigated aerobic bacteria, only a few have investigated anaerobe isolates. the aim of the present study was to determine the antibacterial activity of linezolid against a larger sample of clinical isolates of fusobacterium spp. and to report on the detailed susceptibility, stratified by species.200616905529
mandatory testing? 20061426457
use of the autopsy in northern ireland and its value in perioperative deaths.a report on the pilot study of the confidential enquiry into perioperative deaths was published recently in the united kingdom. the scheme was designed as a specialized form of clinical audit relating to perioperative patient care. because the report placed little emphasis on autopsy, we have looked at all autopsied perioperative deaths, as defined by the enquiry, over a three-year period (1986-88) in a defined catchment area of northern ireland. class i major discrepancies of diagnosis between ...20071855930
insulin secretagogues induce ca(2+)-like changes in cytoplasmic mg2+ in pancreatic beta-cells.the effects of insulin secretagogues on the cytoplasmic mg2+ concentration ([mg2+]i) of pancreatic beta-cells were studied in suspensions and in individual beta-cells using dual-wavelength fluorometry and the indicator mag-fura-2. average [mg2+]i was in the 800-900 microm range in a medium containing 3 mm glucose. when the sugar concentration was raised to 20 mm, the cells reacted with an initial lowering of [mg2+]i followed by an increase. the sugar apparently also stimulated leakage of the mg2 ...20072223873
segmented filamentous bacteria in a defined bacterial cocktail induce intestinal inflammation in scid mice reconstituted with cd45rbhigh cd4+ t cells.the aim was to analyze the influence of intestinal microbiota on the development of intestinal inflammation. we used the model of chronic inflammation that develops spontaneously in the colon of conventional severe combined immunodeficiency (scid) mice restored with the cd45 rb(high) subset of cd4+t cells isolated from the spleen of normal balb/c mice.200717607724
shear bond strength and microleakage of dentin bonding systems.this study evaluated the shear bond strength and microleakage of class v non-retentive restorations with 10 different dentin bonding systems. some were tested with different composite resins. for the shear bond test, eight specimens were prepared for each product and tested in a universal testing machine. for the microleakage test, 158 v-shaped class v cavities extending to the cementoenamel junction were prepared, coated with a dentin bonding system, and restored with a composite resin. after 2 ...20071829112
fifty hours for the poor? 20082612144
[analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. 20082612141
[analysis of the nursing factors responsible for expanding the sphere of daily activities of patients with parkinson's disease]. 20082612141
closed intramedullary nailing of diaphyseal fractures of the femur. problems related to anatomical variations of the greater trochanter.an examination of the radiographs of 100 normal femurs revealed an anatomical variation consisting of lateralization of the trochanteric mass in relation to the femoral diaphysis. this involves disalignment of the apex of the greater trochanter in relation to the medullary canal, with consequent complications during closed intramedullary nailing such as varus of the fracture segments, valgus of the nail, fracture of the medial cortex. since becoming aware of this anatomical variation and its pos ...20092737894
the acid-etch retained rest for removable partial dentures.this article will describe the techniques for composite and metal rests. it will also discuss a clinical follow-up of 18 patients who have had metal or composite rests in place from three to 18 months.20092691082
a century of change in neurosurgery at johns hopkins: 1889-1989.neurosurgical admissions to the johns hopkins hospital during the first year of its existence, the first and last years of harvey cushing's practice, the last years of walter dandy's practice, the last years of earl walker's tenure, and the current neurosurgical case load are reviewed. sixteen patients with neurosurgical problems were admitted during the first year of the hospital's existence. in the first year of harvey cushing's staff appointment, 38 neurosurgical patients were admitted. infec ...20092681565
needle aspiration of nonperitonsillar head and neck abscesses. a six-year experience.twenty-five patients with various head and neck abscesses have been managed with needle aspiration as the initial surgical modality over a six-year period. twenty (83%) of 24 patients' abscesses resolved without formal surgical intervention. patient 25 in the series whose abscess, though initially resolving, reaccumulated within two weeks requiring an incision and drainage. recurrent cancer was then diagnosed. eight of ten large-volume abscesses (over 10 ml) were controlled with needle aspiratio ...20113166766
south african gem of medical history. 20113147784
oesophageal pain exacerbated by propranolol. 20113113570
social work and primary care--the need for increased collaboration: discussion paper. 20123343670
analytical threshold and stability results on age-structured epidemic models with vaccination.this paper examines mathematical models for common childhood diseases such as measles and rubella and in particular the use of such models to predict whether or not an epidemic pattern of regular recurrent disease incidence will occur. we use age-structured compartmental models which divide the population amongst whom the disease is spreading into classes and use partial differential equations to model the spread of the disease. this paper is particularly concerned with an analytical investigati ...20123266036
whose polyp is it, anyway? 20133633101
self-assessment for managers of health care. how can i be a better manager? 20133604457
treatment of idiopathic hypercalciuria with indapamide.twenty-six patients with idiopathic hypercalciuria (urine calcium level greater than 300 mg/24 h [7.5 mmol/d]) were treated with indapamide (a nonthiazide diuretic), 2.5 mg/d for 3 consecutive months. a mean decrease in urine calcium levels of 52% was noted (p less than 0.05). when therapy with indapamide was stopped, the calcium levels returned to pretherapy values. the effect of indapamide on urine calcium levels was similar to that of hydrochlorothiazide in 10 patients who were receiving the ...20133719496
[reconstructive plastic surgery of the humeral condyles following removal of endoprostheses of the elbow versus arthrodesis].the condyles of the elbow are of utmost importance for the stability of this joint. this is particularly evident in cases where elbow prostheses had to be removed because of loosening or infection and where the condyles had been deliberately resected at the primary operation. in order to obtain a functional elbow with sufficient stability and mobility after removal of the prostheses, we can reconstruct the condyles by means of a large autologous bone graft, which we take from the midpart of the ...20133658417
the long-term effects of a token economy on safety performance in open-pit mining.a token economy that used trading stamps as tokens was instituted at two dangerous open-pit mines. employees earned stamps for working without lost-time injuries, for being in work groups in which all other workers had no lost-time injuries, for not being involved in equipment-damaging accidents, for making adopted safety suggestions, and for unusual behavior which prevented an injury or accident. they lost stamp awards if they or other workers in their group were injured, caused equipment damag ...20133667473
[endometriosis in adolescent girls with developmental genital defects]. 20133592085
comparing proportion exposed in case-control studies using several control groups. 20133605045
[the use of occupational law in solving problems at obstetric-gynecologic facilities]. 20133811411
[vectorcardiographic study in patients with pulmonary silicosis]. 20154681283
[studies on visual acuity and color perception in the water]. 20154476181
[cytochemical studies of the fine structure of klossia helicina (coccidia, adeleidea). i. morphology and cultivation of klossia helicina]. 20164995337
preparation of heparin-linked agarose and its interaction with plasma. 20164831002
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