Publications

an investigation of the optimal conditions for the in vivo production of immunologically sensitised rat mast cells.	experiments were designed to develop an optimal method for inducing in vivo production of sensitised peritoneal mast cells. rats of different strains were sensitised with whole egg-white and killed at suitable intervals to harvest the peritoneal mast cells. release of histamine was induced in vitro by both whole egg-white and its major protein constituents, and assayed by a standard spectrofluorometric method. wistar rats showed higher levels of sensitisation than black-hooded lister rats; it wa ...	1979	92507
the effect of dose loading and of double labelling with 57co and 125i on the tissue distribution in animals.	dose loading effects upon the performance of 57co-bleomycin as a tumor localizing agent have been investigated in rhabdomyosarcoma bearing wag/ry rats. the addition of non-radioactively labelled co-bleomycin increased the relative uptake of 57co-bleomycin in rapid growing tumors, but the addition of non-chelated bleomycin had no influence at all. in our experimental system, iodinated bleomycin generally labelled by reaction with icl, was found to be an unsatisfactory tumor localizing agent. in o ...	1979	93048
differential location of different types of intermediate-sized filaments in various tissues of the chicken embryo.	the location of constitutive proteins of different types of intermediate-sized (about 10 mm) filaments (cytokeratin, vimentin, desmin, brain filament protein) was examined in various tissues of 11--20 day chick embryos, using specific antibodies against the isolated proteins and immunofluorescence microscopy on frozen sections and on isolated serous membrane. the tissues studied which contained epithelia were small intestine, gizzard, esophagus, crop, liver, kidney, thymus, mesenteries, and epid ...	1979	93557
measurement of androgen receptor in cytosol of human prostatic tissues with a sepharose-linked antibody system.		1979	93753
purification of antibodies to influenza a virus structural proteins by affinity chromatography, and their participation in haemagglutination-inhibition, neutralization and enzyme-linked immunosorbent assay.	affinity chromatography was used to purify rabbit antibodies to common and strain-specific antigeneic determinants of haemagglutinin, to neuraminidase, and to a combination of the internal proteins of influenza a viruses. the purity of the antibodies was assessed by haemagglutination-inhibition, enzyme-linked immunosorbent assay (elisa) and competition elisa. the antibodies were examined for their participation in neutralization and haemagglutination-inhibition assays, and in elisa. elisa was fo ...	1979	93960
changes in lung ultrastructure following heterologous and homologous serum albumin infusion in the treatment of hemorrhagic shock.	the object of this study was to compare the ultrastructure pulmonary effects of the infusion of homologous and heterologous serum albumin solution in the treatment of hemorrhagic shock in baboons. adult baboons subjected to hemorrhagic shock were resuscitated with either baboon serum albumin, human serum albumin, or ringer's lactate solution. the lungs were fixed in vivo with potassium pyroantimony, a solution which produces electron dense interstitial precipitation of sodium. the lungs from ani ...	1979	106780
[radioautographic identification of dopaminergic axons in the rat cns. in vitro marking with tritiated dopamine or noradenaline].	a new radioautographic technique for the visualization of central dopaminergic fibers is described. this technique is based upon in vitro uptake of triatiated norepinephrine or tritiated dopamine in the presence of monoamine oxidase inhibitor and specific uptake inhibitor in noradrenergic fibers. after a short step in glutaraldehyde, the use of osmium vapours as a fixative, instead of osmic acid immersion, allows a satisfactory retention of triatiated amines within dopaminergic fibers.	1979	120791
amino acid uptake systems in bacteroides ruminicola.	uptake of amino acids by bacteroides ruminicola was observed in cells grown in a complete defined medium, containing ammonia as the nitrogen source. a high rate of uptake occurred only in fresh medium, as an inhibitory substance, possibly acetate, apparently accumulated during growth. all amino acids except proline were taken up and incorporated into cold trichloroacetic acid precipitable material. different patterns of incorporation and different responses to 2,4-dinitrophenol and potassium fer ...	1979	575310
comparison of substrate affinities among several rumen bacteria: a possible determinant of rumen bacterial competition.	five rumen bacteria, selenomonas ruminantium, bacteroides ruminicola, megasphaera elsdenii, streptococcus bovis, and butyrivibrio fibrisolvens were grown in continuous culture. estimates of substrate affinities were derived from lineweaver-burk plots of dilution rate versus substrate concentration. each bacterium was grown on at least four of the six substrates: glucose, maltose, sucrose, cellobiose, xylose, and lactate. wide variations in substrate affinities were seen among the substrates util ...	1979	16345358
comparison of maintenance energy expenditures and growth yields among several rumen bacteria grown on continuous culture.	maintenance energy expenditures were mesured for five rumen bacteria, selenomonas ruminantium, butyrivibrio fibrisolvens, bacteroides ruminicola, megasphaera elsdenii, and streptococcus bovis, by using a complex medium with glucose as the carbon source. large differences (as high as 8.5-fold) in maintenance energy expenditures were seen among these bacteria. the suggestion is made that maintenance requirements could be a significant determinant of bacterial competition in the rumen. theoretical ...	1979	16345359
effects of combinations of substrates on maximum growth rates of several rumen bacteria.	five rumen bacteria, selenomonas ruminantium, bacteroides ruminicola, megasphaera elsdenii, butyrivibrio fibrisolvens, and streptococcus bovis were grown in media containing nonlimiting concentrations of glucose, sucrose, maltose, cellobiose, xylose and/or lactate. each bacterium was grown with every substrate that it could ferment in every possible two-way combination. only once did a combination of substrates result in a higher maximum growth rate than that observed with either substrate alone ...	1979	16345360
effect of monensin and lasalocid-sodium on the growth of methanogenic and rumen saccharolytic bacteria.	it is thought that monensin increases the efficiency of feed utilization by cattle by altering the rumen fermentation. we studied the effect of monensin and the related ionophore antibiotic lasalocid-sodium (hoffman-laroche) on the growth of methanogenic and rumen saccharolytic bacteria in a complex medium containing rumen fluid. ruminococcus albus, ruminococcus flavefaciens, and butyrivibrio fibrisolvens were inhibited by 2.5 mug of monensin or lasalocid per ml. growth of bacteroides succinogen ...	1979	16345418
[concentration of serum iron and transferrin saturation with iron as indices of changes resulting from chronic exposure to chemicals in industry].	an attempt was undertaken to use the following indices of systemic changes resulting from longlasting occupational exposure to some chemicals: -- ferrum concentration in blood serum, the level of latent capacity to bind ferrum, and the degree of transferrin saturation with ferrum. the examination involved 177 clinically healthy men, aged 18--60. the control group consisted of 40 healthy men, aged 22--60. in 30.5% of persons, deviations were found, which might be induced by disturbances in genera ...	1979	216880
the essence of operating room nursing. the problem of isolation.		1979	243437
therapeutic groups - a clue to management.		1979	243426
nursing care study: joint replacement.		1979	243252
any complaints?		1979	243251
nursing care supplement series b. specialised care. 10. microvascular surgery.		1979	243250
gram-negative anaerobic bacilli: their role in infection and patterns of susceptibility to antimicrobial agents. i. little-known bacteroides species.	the role in infection and the patterns of susceptibility to antimicrobial agents of 17 infrequently reported species of bacteroides were studied. the findings of this research are summarized, and the literature dealing with recovery of these species from both the normal flora and infections of humans is reviewed. during a six-year period, 22% of 679 specimens from infections due to anaerobic bacteria that were studied by the authors yielded one or more of these "other bacteroides species" (obs). ...	1980	7012991
utilization of ammonia nitrogen by intestinal bacteria isolated from pigs.	in a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 bacteroidaceae strains, 3 of selenomonas strains, 1 of 7 curved rods, 3 of 7 spirochaetaceae strains, 8 of 20 eubacterium strains, 8 of 13 peptococcaceae strains, 3 of 4 clostridium strains, 19 of 20 enterobacteriaceae strains, and 1 of 8 streptococcus strains utilized ammonia nitrogen preferentially to proteose peptone nitrogen. to determine the ability of intestinal microbes to synthesize amino acids f ...	1980	7356320
effect of ph on the efficiency of growth by pure cultures of rumen bacteria in continuous culture.	a total of 10 strains of rumen bacteria, selenomonas ruminantium hd4, megasphaera elsdenii b159, butyrivibrio fibrisolvens a38, streptococcus bovis jb1, lactobacillus vitulinus ga1, bacteroides ruminicola b14, b. ruminicola ga33, ruminococcus albus 7, ruminococcus flavefaciens c94, and bacteroides succinogenes s85, were grown in energy-limiteh of the medium reservoir was lowered approximately 0.3 ph units, and the energy source concentration remaining in the culture vessel, optical density, cell ...	1980	7387158
the constitutive nature of alkaline phosphatase in rumen bacteria.	alkaline phosphatase (apase) activity of megasphaera elsdenii was enhanced by po4 2- limitation in batch culture; however, six other species of rumen bacteria tested showed no increase in apase activity under these conditions. alkaline phosphatase was produced by the mixed rumen microflora even though the inorganic phosphorus concentration was as high as 10mm. the apase activity of the bacterial fraction from rumen fluid was not increased during incubation in a phosphorus-free culture medium. si ...	1980	7407707
ammonia saturation constants for predominant species of rumen bacteria.	ammonia saturation constants were determined for representative pure cultures of predominant, anaerobic, fermentative rumen bacteria. based on growth experiments with ammonia limited continuous cultures, average estimates for ammonia saturation constants of bacteroides amylophillus and bacteroides ruminicola were 10.5 and 23.5 microm ammonia-nitrogen, respectively. with ammonia-limited linear-growth cultures, the estimates for the ammonia saturation constants of b. amylophilus, b. ruminicola, an ...	1980	7419777
the influence of extracellular hydrogen on the metabolism of bacteroides ruminicola, anaerovibrio lipolytica and selenomonas ruminantium.	strains of three anaerobic rumen bacteria, bacteroides ruminicola, anaerovibrio lipolytica and selenomonas ruminantium, were able to use extracellular h2 to reduce fumarate to succinate. each bacterium possessed membrane-bound hydrogenase and fumarate reductase activity. membrane-bound cytochrome b was reducible by h2 and oxidizable by fumarate in each bacterium. the apparent km values for hydrogen of the hydrogenases were 4 . 5 x 10(-6) m, 1 . 4 x 10(-5) m and 4 . 4 x 10(-5) m for b. ruminicola ...	1980	6785381
anaerobic bacteria in dentoalveolar infections.	the bacteriology of 57 dentoalveolar infections was studied using optimal techniques to collect, transport and process specimens. there was an average of 4 bacterial species per specimen, and only 1/3 of the specimens held aerobes. among the aerobic bacteria, streptococci dominated and among the anaerobes the gram-negative rods, bacteroides ruminicola and fusobacterium nucleatum, were most frequently isolated followed by gram-positive cocci, in particular streptococcus intermedius. all aerobic i ...	1981	6807917
hydrolysis of leaf fraction 1 protein by the proteolytic rumen bacterium bacteroides ruminicola r8/4.	proteolytic activity in a batch culture of bacteroides ruminicola r8/4 was maximal and largely (greater than 90%) cell-associated during the mid-exponential phase of growth. the cell-bound protease was not inactivated during storage at --70% c, was not significantly affected by ph over the range 5.9 to 8.2, but was subject to substrate inhibition by fraction 1 protein (ribulose-1,5-bisphosphate carboxylase; ec 4.1.1.39) and was most active in the presence of thiol reagents. radioactive fraction ...	1981	7033457
proteolytic activities of a rumen bacterium, bacteroides ruminicola r8/4.	proteolysis of leaf fraction 1 protein, casein, azocasein and bovine serum albumin by the cell-associated proteinases of the rumen bacterium bacteroides ruminicola r8/4 was investigated and the kinetic parameters vmax and km were evaluated for each substrate. a variety of proteinase inhibitors was used to show that the proteolytic activity comprises a mixture of at least three different classes of proteinase. with respect to substrate specificity and some inhibition characteristics, the proteoly ...	1981	7038030
bacterial population adherent to the epithelium on the roo of the dorsal rumen of sheep.	by anaerobic procedures, the total number of adherent bacteria was determined on tissue samples obtained from the roof of the dorsal rumen of three sheep. after four washings, 1.91 x 10, 0.34 x 10, and 1.23 x 10 bacteria per cm were still attached to the rumen epithelium in sheep 1, 2, and 3, respectively. a total of 95 strains of bacteria were isolated from these three samples. based on morphology, gram stain, anaerobiosis, motility, and fermentation end products, they were presumptively identi ...	1981	16345797
effects of long-chain fatty acids on growth of rumen bacteria.	the effects of low concentrations of long-chain fatty acids (palmitic, stearic, oleic, and vaccenic) on the growth of seven species (13 strains) of rumen bacteria were investigated. except for bacteroides ruminicola and several strains of butyrivibrio fibrisolvens, bacterial growth was not greatly affected by either palmitic or stearic acids. in contrast, growth of selenomonas ruminantium, b. ruminicola, and one strain of b. fibrisolvens was stimulated by oleic acid, whereas the cellulolytic spe ...	1981	16345887
characterization of the cecal bacteria of normal pigs.	one hundred ninety-two isolates from cecal contents of three normal weaned pigs were obtained by means of anaerobic roll tube methods and were characterized. seventy-eight percent of the isolates were gram-negative. the most numerous species isolated from each of the pigs was bacteroides ruminicola. this species accounted for 35% of the isolates that were characterized, and selenomonas ruminantium accounted for 21% of the isolates. other gram-negative bacteria isolated from all three pigs were b ...	1981	7235711
a note on the fermentation of pectin by pure strains and combined cultures of rumen bacteria.	bacteroides ruminicola, pure or combined with selenomonas ruminantium, and lachnospira multiparus, pure or combined with succinivibrio dextrinosolvens, were grown on a medium with pectin as energy source. there was a difference in fermentation products between the pure and combined cultures and efficiency of substrate utilization was better with the combined cultures.	1981	6168176
[nursing, does the public know it?].		1981	243934
temporal arteritis and polymyalgia rheumatica.		1981	243839
bacteroides ruminicola pyonephrosis.		1982	7116113
tetrapyrrole utilization of bacteroides fragilis.	during growth in a defined medium containing protoheme, bacteroides fragilis strain 2044 nd bacteroides "l" 7cm formed protoheme-containing b-type cytochromes. the major reduced versus oxidized spectral peaks found for strain 2044 were at 562 and 428 nm whereas the peaks observed for strain 7cm were at 559 and 427 nm. when protoheme was replaced by deuteroporphyrin, mesoporphyrin, or their manganese or magnesium chelates, both organisms formed deuteroheme- or mesoheme-containing cytochromes with ...	1982	7168828
dehydrogenase patterns in the taxonomy of bacteroides.	the malate dehydrogenase (mdh) electrophoretic mobilities of 128 strains of bacteroides belonging to 17 species, including three subspecies of bacteroides melaninogenicus and two subspecies of bacteroides ruminicola, were examined. amongst the pigmented bacteroides, the migration of this enzyme correlated well with recognized taxa, and only one strain, vpi 9085 was clearly different. other species such as b. oralis, b. buccalis, b. denticola, b. pentosaceus, b. bivius, b. disiens and b. ruminico ...	1982	7183748
fermentation of peptides by bacteroides ruminicola b(1)4.	the maximum growth rate of bacteroides ruminicola b(1)4 was significantly improved when either trypticase or acetate and c(4)-c(5) fatty acids were added to defined medium containing macrominerals, microminerals, vitamins, hemin, cysteine hydrochloride, and glucose. the organism was unable to grow with peptides as the sole energy source, but growth yields from glucose were significantly improved when trypticase was added to batch cultures containing basal medium, acetate, and c(4)-c(5) volatile ...	1983	16346292
infectivity of organisms recovered from polymicrobial abscesses.	the ability to cause subcutaneous abscesses in mice was used to identify the pathogens among the bacteria recovered from 13 clinical abscesses. a total of 35 isolates (30 anaerobes and 5 aerobes), 16 of which were encapsulated, were recovered from these abscesses. encapsulated organisms included eight bacteroides spp. (three bacteroides asaccharolyticus and one strain each of bacteroides oralis, bacteroides intermedius, bacteroides biacutus, bacteroides vulgatus, and bacteroides ruminicola subsp ...	1983	6642674
alpha-ketoglutarate metabolism by cytochrome-containing anaerobes.	during growth in the presence of tracer amounts of exogenously supplied alpha-keto[1-14c]glutarate (akg) or alpha-keto [5-14c]glutarate, cytochrome-containing bacteroides fragilis strain 2044 and bacteroides vulgatus strain 8482 incorporated extremely small amounts of radioactivity into cell macromolecules and protoheme. under identical conditions, bacteroides "l" strain 7cm and bacteroides buccae strain j1 incorporated substantial label from [5-14c]akg, but not [1-14c]akg, into cellular macromo ...	1983	6413047
[process and development of a rural practice in a program of licensure in nursing. experiences].		1983	242502
alternative pathways for biosynthesis of leucine and other amino acids in bacteroides ruminicola and bacteroides fragilis.	bacteroides ruminicola is one of several species of anaerobes that are able to reductively carboxylate isovalerate (or isovaleryl-coenzyme a) to synthesize alpha-ketoisocaproate and thus leucine. when isovalerate was not supplied to growing b. ruminicola cultures, carbon from [u-14c]glucose was used for the synthesis of leucine and other cellular amino acids. when unlabeled isovalerate was available, however, utilization of [u-14c]glucose or [2-14c]acetate for leucine synthesis was markedly and ...	1984	6440485
taxonomy of anaerobes: present state of the art.	results of nucleic acid studies, which indicate relationships among strains and species more clearly than do usual phenotypic tests, have led to new bacteriologic nomenclature. some major changes in bacteroides include the recognition of three species (bacteroides melaninogenicus, bacteroides denticola, and bacteroides loescheii) formerly grouped in b. melaninogenicus subspecies melaninogenicus; two species (bacteroides intermedius with two closely related homology groups and bacteroides corpori ...	1984	6201990
role of anaerobic bacteria in bite-wound infections.	the etiologic agents usually involved in wound infections due to human or animal bites are the aerobic skin flora of the victim, e.g., staphylococcus aureus, and/or the aerobic oral flora of the biter, e.g., pasteurella multocida. while anaerobic bacteria are predominant in the normal oral flora of humans and animals, their importance in the pathogenesis of bite-wound infections has not been stressed. most investigators in this field have either not cultured these wounds for anaerobic bacteria o ...	1984	6372026
the role of beta-lactamase-producing bacteria in the persistence of streptococcal tonsillar infection.	the failure of penicillin to eradicate group a beta-hemolytic streptococci (gabhs) is a growing problem. this review summarizes current data concerning the role of aerobic and anaerobic beta-lactamase-producing bacteria in the persistence of tonsillar infection caused by gabhs. clinical studies have demonstrated the recovery of beta-lactamase-producing bacteria and detectable levels of the enzyme beta-lactamase in cultures of core tonsillar specimens in greater than 80% of the patients. the pred ...	1984	6390637
enrichment and isolation of rumen bacteria that reduce trans- aconitic acid to tricarballylic acid.	bacteria from the bovine rumen capable of reducing trans-aconitate to tricarballylate were enriched in an anaerobic chemostat containing rumen fluid medium and aconitate. after 9 days at a dilution rate of 0.07 h, the medium was diluted and plated in an anaerobic glove box. three types of isolates were obtained from the plates (a crescent-shaped organism, a pleomorphic rod, and a spiral-shaped organism), and all three produced tricarballylate in batch cultures that contained glucose and trans-ac ...	1985	16346691
human pharmacokinetic and pharmacodynamic studies on ro31-1118, a new beta-adrenoceptor antagonist.	the pharmacokinetic and pharmacodynamic effects of ro31-1118 were examined in groups of healthy volunteers. in three subjects given 10 mg of [14c]-ro31-1118 orally, peak levels of radioactivity (84 +/- 5 ng/ml) were 16 times those of the parent drug (approximately 5 ng/ml). very little parent drug was recovered in the urine, although recovery of total radioactivity was nearly 80% in the urine by day 5. in five subjects studied after both oral and intravenous administration of 20 mg ro31-1118 the ...	1985	2859047
induction of muscarinic cholinergic responsiveness in xenopus oocytes by mrna isolated from rat brain.	microinjection of mrna from adult rat brains into xenopus oocytes induced an electrophysiological response to acetylcholine in the cells. the cells responded to acetylcholine through muscarinic receptors which appeared on the cell surface after microinjection of the mrna and showed the depolarization, mainly due to an increase in membrane permeability to cl ions. the acetylcholine reaction did not require extracellular ca ions and was suppressed by pertussis toxin, suggesting the involvement of ...	1985	3928090
purification and properties of a protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex.	the protein activator of phosphorylated branched-chain 2-oxo acid dehydrogenase complex was purified greater than 1000-fold from extracts of rat liver mitochondria; the specific activity was greater than 1000 units/mg of protein (1 unit gives half-maximum re-activation of 10 munits of phosphorylated complex). sodium dodecyl sulphate/polyacrylamide-gel electrophoresis gave two bands (mr 47700 and 35300) indistinguishable from the alpha- and beta-subunits of the branched-chain dehydrogenase compon ...	1985	3977842
distribution and localization of endotoxin in the reticulo-endothelial system (res) and in the main vessels of the rat during shock.	the fate of endotoxin was followed with immunohistochemistry and radio-labelled lipopolysaccharide (lps) in organs of the reticulo-endothelial system (res), in the great vessels and the thoracic duct of rats during a 14 day period after the injection of a shock-inducing amount of endotoxin. the immunohistochemical detectability of lps in most tissues increased continuously during the first 48 hours, showing the strongest lps staining in the liver and adrenal gland. macrophages were found to be t ...	1985	4001029
determination of mercury and organomercurial resistance in obligate anaerobic bacteria.	a methodology for determining the minimum inhibitory concentration of inorganic and organomercurial compounds for obligate anaerobic bacteria is described. a wide variation in the susceptibility of anaerobic clinical and sewage isolates was observed. isolates of bacteroides ruminicola and clostridium perfringens resistant to mercury were examined for their plasmid content and ability to demonstrate inducible resistance. none of the resistant anaerobes contained any plasmids, while resistant facu ...	1985	4005712
x-ray tv system for measuring microcirculation in small pulmonary vessels.	we developed a new system that consists of 1) a specially designed x-ray apparatus, 2) an x-ray-sensitive 1-in. vidicon camera, and 3) a digital image-processing device. the picture element is approximately 20 micron in size, and the time required for one frame is 1/30 s. using this system, we measured the internal diameter (id), the cross-sectional area, flow velocity, volume flow, and transit time of small pulmonary vessels of approximately 100-500 micron at control and with serotonin in anest ...	1985	4055558
antibiotic activity of an isocyanide metabolite of trichoderma hamatum against rumen bacteria.	a metabolite of trichoderma hamatum, 3-(3-isocyanocyclopent-2-enylidene)propionic acid, was tested for its effects on growth of and carbohydrate metabolism in 11 strains of functionally important rumen bacteria. to standardize the biological activity of this unstable metabolite, a rapid, aerobic disc diffusion assay was developed using escherichia coli atcc 11775. in an anaerobic broth dilution assay using a medium lacking rumen fluid and containing a soluble carbohydrate, the minimum inhibitory ...	1985	4084861
the protective effect of different drugs in rats poisoned by sulfur and nitrogen mustards.	based on the recent pathognomic accomplishment in the mechanisms of toxic actions, the favorable degree of protection was achieved by several drugs in acute poisoning of rats with sulfur and nitrogen mustards. it has been established that dexamethasone, promethazine, vitamin e, heparin, and sodium thiosulfate (a) prolonged the survival time in animals poisoned by 3 ld50s of toxic agents, (b) diminished the lethalty (with the protective indices ranging from 1.5 to 2.7), (c) antagonized the decrea ...	1985	4092884
ultrastructural observations on the sporogony of eimeria maxima.		1985	4093234
increased gastric cholinergic activity evoked by 5-hydroxy-l-tryptophan in the rat.	in gastrointestinal tissues such as rat stomach, exogenous 5-hydroxytryptamine (5ht) has little or no ability to affect nerve activity. however, endogenous 5ht might act differently, and this was investigated by stimulating 5ht synthesis using 5-hydroxy-l-tryptophan (5htp). in longitudinal strips of rat forestomach, 5htp (50 and 500 microm) increased cholinergically mediated contractions evoked by electrical field stimulation, probably by facilitating acetylcholine release; contractions evoked b ...	1986	3019729
increased gastric cholinergic activity evoked by 5-hydroxy-l-tryptophan in the rat.	in gastrointestinal tissues such as rat stomach, exogenous 5-hydroxytryptamine (5ht) has little or no ability to affect nerve activity. however, endogenous 5ht might act differently, and this was investigated by stimulating 5ht synthesis using 5-hydroxy-l-tryptophan (5htp). in longitudinal strips of rat forestomach, 5htp (50 and 500 microm) increased cholinergically mediated contractions evoked by electrical field stimulation, probably by facilitating acetylcholine release; contractions evoked b ...	1986	3019729
chronic osteomyelitis of mandible caused by penicillin-resistant bacteroides ruminicola. report of a case.	osteomyelitis of the jaws caused by a bacteroides species is uncommon. a case of osteomyelitis of the mandible caused by penicillin-resistant bacteroides ruminicola is reported. the diagnosis was confirmed by isolation of the organism and bone scan (99mtc medronate disodium) of the mandible. clindamycin and hyperbaric oxygen therapy resolved the infection.	1986	3456137
fodrin is part of a filamentous cortical sheath of the detergent resistant cytoskeleton of cultured cells before and after cytochalasin treatment.	cytoskeletons of cultured cells prepared under mild conditions in the presence of "stabilization' buffer contain most of the fodrin present in the cells. the fodrin in these cytoskeletons was localized by immunofluorescence microscopy and found to be present in a cortical sheath of fine filaments. in general, the filamentous distribution showed no correspondence with actin bundles as revealed by double-label fluorescence microscopy. however, in cells with large and abundant stress fibers, some c ...	1986	3514218
proteolytic activity of the ruminal bacterium butyrivibrio fibrisolvens.	the proteolytic activity of butyrivibrio fibrisolvens, a ubiquitously distributed bacterial species in the gastrointestinal tracts of ruminants and other mammals, was characterized. the relative proteolytic activity (micrograms of azocasein degraded per hour per milligram of protein) varied greatly with the strain: 0 to 1 for strains d1, d16f, e21c, and x6c61; 7 to 15 for strains il631, nor37, s2, lm8/1b, and x10c34; and 90 to 590 for strains 12, 49 h17c, cf4c, cf3, cf1b, and r28. the activity l ...	1986	3524460
splenic hematoma and abscess as a cause of chronic weight loss in a horse.	an 8-year-old gelding with a 3-month history of anorexia and weight loss was found to have a massive subcapsular splenic hematoma. at flank laparotomy, 36 l of fluid was removed from the hematoma. the horse's condition improved after drainage. fifteen months later, the horse became depressed and febrile. a splenic abscess containing bacteroides ruminicola and clostridium sporogenes was found at necropsy.	1986	3531125
[the tenacity of bacteria in the airborne state. iv: experimental studies on the viability of airborne e. coli 0:78 under the influence of different temperature and humidity].	in a static aerosol chamber the tenacity of airborne e. coli 0:78 was determined at a permanent temperature of 22 degrees c and different relative humidities (10, 15-20, 30, 75 and 85%) and also at a permanent humidity of 30-40% and different temperatures (22, 28, 30, 34 and 40 degrees c). the greatest viability of the bacteria with a half-life-time of 390 min was found at 22 degrees c and 85% humidity. at 10% humidity the half-life-time was only 55 min. at a humidity of 30-40% the tenacity decr ...	1986	3538718
18q+, the progeny of a balanced translocation t(1;18)mat: case report with necropsy findings.	a female infant with additional genetic material on the long arm of chromosome 18 is described. cytogenetic studies of the infant and her mother showed that the altered region resulted from an unbalanced translocation of part of the long arm of chromosome 1. this chromosomal abnormality has not been reported previously, according to a recent registry of abnormal chromosome patterns. the patient had hydrops fetalis and multiple congenital abnormalities, involving the cardiovascular, respiratory, ...	1986	3723558
heat production by ruminal bacteria in continuous culture and its relationship to maintenance energy.	selenomonas ruminantium hd4 and bacteroides ruminicola b(1)4 were grown in continuous culture with glucose as the energy source, and heat production was measured continuously with a microcalorimeter. because the bacteria were grown under steady-state conditions, it was possible to calculate complete energy balances for substrate utilization and product formation (cells, fermentation acids, and heat). as the dilution rate increased from 0.04 to 0.60 per h, the heat of fermentation declined from 1 ...	1986	3782021
phosphoenolpyruvate-dependent phosphorylation of hexoses by ruminal bacteria: evidence for the phosphotransferase transport system.	six species of ruminal bacteria were surveyed for the phosphoenolpyruvate (pep)-dependent phosphorylation of glucose. selenomonas ruminantium hd4, streptococcus bovis jb1, and megasphaera elsdenii b159 all showed significant activity, but butyrivibrio fibrisolvens 49, bacteroides succinogenes s85, and bacteroides ruminicola b1(4) showed low rates of pep-dependent phosphorylation and much higher rates in the presence of atp. s. ruminantium hd4, s. bovis jb1, and m. elsdenii b159 also used pep to ...	1986	3789722
hypergastrinemia after blockade of acid secretion in the rat: trophic effects.	the availability of potent and long-acting blockers of acid secretion, such as omeprazole, has paved the way for experimental studies on the long-term effects of permanently raised levels of circulating gastrin without the complication of surgical intervention. we have examined rats given high doses of the antisecretagogues omeprazole and ranitidine during 10 or 20 weeks for general trophic effects on the gastrointestinal tract and pancreas and for the effects on endocrine cells such as the soma ...	1986	3792672
estradiol-binding proteins from mycelial and yeast-form cultures of paracoccidioides brasiliensis.	paracoccidioides brasiliensis, the etiologic agent of paracoccidioidomycosis, causes disease much more frequently in men than it does in women, suggesting that the hormonal milieu of the host might influence p. brasiliensis pathogenicity. we recently demonstrated that cytosol from yeast cultures of p. brasiliensis contains a high-affinity, low-capacity, tritiated 17 beta-estradiol [( 3h]estradiol)-binding protein. estradiol and, to a lesser degree, diethylstilbestrol (des), inhibited the transfo ...	1986	3079729
myasthenia in patients with dermatomyositis: clinical, electrophysiological and ultrastructural studies.	in 4 patients with clinical signs of dermatomyositis, confirmed by electromyography and muscle biopsy, a form of muscle fatigue was detected which was expressed clinically by predominantly proximal motor deficit, with phonation and deglutition disturbances, slightly influenced by prostigmine. in all patients, stimulation of the ulnar nerve at 3--10 hz induced a decrement of muscle-evoked potentials in abductor digiti minimi and at 15--50 hz an increment at the end of the trains (1.2 sec in durat ...	1986	213537
riojun kinosita: 1893-1977.		1986	203391
[breast eczema and breast neoplasms. pseudo paget's disease of the breast].		1987	204113
susceptibility and resistance of ruminal bacteria to antimicrobial feed additives.	susceptibility and resistance of ruminal bacterial species to avoparcin, narasin, salinomycin, thiopeptin, tylosin, virginiamycin, and two new ionophore antibiotics, ro22-6924/004 and ro21-6447/009, were determined. generally, antimicrobial compounds were inhibitory to gram-positive bacteria and those bacteria that have gram-positive-like cell wall structure. mics ranged from 0.09 to 24.0 micrograms/ml. gram-negative bacteria were resistant at the highest concentration tested (48.0 micrograms/ml ...	1987	3116929
regulation of beta-glucosidase in bacteroides ruminicola by a different mechanism: growth rate-dependent derepression.	bacteroides ruminicola b(1)4, a predominant ruminal and cecal bacterium, was grown in batch and continuous cultures, and beta-glucosidase activity was measured by following the hydrolysis of p-nitrophenyl-beta-glucopyranoside. specific activity was high when the bacterium was grown in batch cultures containing cellobiose, mannose, or lactose (greater than 286 u/g of protein). activity was reduced approximately 90% when the organism was grown on glucose, sucrose, fructose, maltose, or arabinose. ...	1987	3122655
fermentation of xylans by butyrivibrio fibrisolvens and other ruminal bacteria.	the ability of butyrivibrio fibrisolvens and other ruminal bacteria (6 species, 18 strains) to ferment a crude xylan from wheat straw or to ferment xylans from larchwood or oat spelts was studied. liquid cultures were monitored for carbohydrate utilization, cell growth (protein), and fermentation acid production. b. fibrisolvens 49, h17c, actf2, and d1 grew almost as well on one or more of the xylans as they did on cellobiose-maltose. b. fibrisolvens 12, r28, a38, x10c34, ard22a, and x6c61 exhib ...	1987	3124741
interleukin-2 receptor-inducing factor(s) in adult t cell leukemia.	the expression of the interleukin-2 receptor (il-2-r) is regulated by transcriptional and post-transcriptional mechanisms. il-2-r gene expression is induced by pharmacological agents including calcium ions, phorbol esters such as phorbol myristate acetate (pma) and forskolin (fk), a direct activator of adenylate cyclase. htlv-i(+) leukemic t cells and t cell lines from patients with adult t cell leukemia (atl) continuously expressed il-2-r without production of il-2. however, there was no abnorm ...	1987	2893506
regional distribution of metals in human brain.	progress in understanding the role of metals in diseases of the nervous system has been hampered to a large extent by a lack of normal metal concentration values in the human brain. since several metals interact metabolically, concurrent metal levels are essential for clinical correlation. we are reporting a simultaneous analysis of 24 metals in 4 different areas of 9 human brains. our data on previously studied metals are comparable to the past observations. therefore, we suggest the values of ...	1987	3815930
bacteriologic and clinical study of bacteroides oris and bacteroides buccae.	we characterized clinical isolates previously identified in our laboratory as bacteroides ruminicola, the human strains of which are now classified as bacteroides oris and bacteroides buccae. a total of 72 isolates (55 b. buccae isolates and 17 b. oris isolates) recovered over a 10-year period were studied. they were differentiated from each other by special-potency antibiotic disks and the rapid-ana system. the two organisms were associated with a variety of infections, the majority being pleur ...	1987	3571453
seasonal changes in the cecal microflora of the high-arctic svalbard reindeer (rangifer tarandus platyrhynchus).	the dominant cecal bacteria in the high-arctic svalbard reindeer were characterized, their population densities were estimated, and cecal ph was determined in summer, when food quality and availability is good, and in winter, when it is very poor. in summer the total culturable viable bacterial population was (8.9 +/- 5.3) x 10(8) cells ml-1, whereas in winter it was (1.5 +/- 0.7) x 10(8) cells ml-1, representing a decrease to 17% of the summer population density. of the dominant species of cult ...	1987	3030193
effects of potassium ion concentrations on the antimicrobial activities of ionophores against ruminal anaerobes.	the antimicrobial activities of monensin and lasalocid against representative strains of ruminal bacteria were evaluated in medium containing three different concentrations of potassium (1.3, 7.9, or 23.3 mm). the growth of eubacterium ruminantium was inhibited by low concentrations of ionophores (less than or equal to 0.16 mg/liter), while the strain of streptococcus bovis tested was resistant to high concentrations of ionophores (40 mg/liter) at all potassium concentrations tested. the mics of ...	1987	3426214
interactions between rumen amylolytic and lactate-utilizing bacteria in growth on starch.	the growth and metabolism of the rumen amylolytic bacteria streptococcus bovis, butyrivibrio fibrisolvens and bacteroides ruminicola, growing in pure cultures and co-cultures with the rumen lactilytic bacteria megasphaera elsdenii and veillonella alcalescens were followed. the interaction of amylolytic bacteria with v. alcalescens represents a simple food chain. the interaction with m. elsdenii is more complex, since there is a simultaneous competition for products of the starch degradation.	1987	3429358
a new rflp at the human vitamin-d binding protein (hdbp) locus.		1988	2901718
fermentation of peptides and amino acids by a monensin-sensitive ruminal peptostreptococcus.	a monensin-sensitive ruminal peptostreptococcus was able to grow rapidly (growth rate of 0.5/h) on an enzymatic hydrolysate of casein, but less than 23% of the amino acid nitrogen was ever utilized. when an acid hydrolysate was substituted for the enzymatic digest, more than 31% of the nitrogen was converted to ammonia and cell protein. coculture experiments and synergisms with peptide-degrading strains of bacteroides ruminicola and streptococcus bovis indicated that the peptostreptococcus was u ...	1988	2975156
plasmid-associated transfer of tetracycline resistance in bacteroides ruminicola.	tetracycline resistance was transferred at frequencies between 10(-7) and 10(-6) per recipient cell in anaerobic matings between two strains of the strictly anaerobic rumen bacterium bacteroides ruminicola. the donor strain, 223/m2/7, was a multiple-plasmid-bearing tetracycline-resistant strain from the ovine rumen, and the recipient, f101, was a rifampin-resistant mutant of b14, a bovine strain belonging to b. ruminicola subsp. brevis. resistance transfer could occur in the presence of dnase, b ...	1988	2837147
monoclonal-antibody-mediated enzyme-linked immunosorbent assay for detection of reticuloendotheliosis viruses.	an enzyme-linked immunosorbent assay (elisa) is described for the detection of reticuloendotheliosis viruses (revs). the assay uses a mixture of monoclonal antibodies (mcas) prepared against a 62-kilodalton rev envelope glycoprotein (gp62) to capture antigen, rabbit anti-rev serum as detection antibody, and peroxidase-conjugated anti-rabbit igg as indicator antibody. the mcas were reactive with rev strain t, chick syncytial virus, and duck infectious anemia virus but unreactive against marek's d ...	1988	2838016
[therapy of aids].	therapeutic strategies in the management of aids should aim at an inhibition of the replication and spread of hiv; the reconstitution of immune functions; an effective chemotherapy of opportunistic infections and tumors; and the prevention of new opportunistic infections. at present, these goals can be only partially achieved and where beneficial therapeutic results are obtained they are only transient in nature. antiretroviral therapy and chemotherapy of infections have, however, resulted in so ...	1988	2849247
proteolysis of alcohol-treated soybean meal proteins by bacteroides ruminicola, bacteroides amylophilus, pepsin, trypsin, and in the rumen of steers.	sodium dodecyl sulfate-gel electrophoresis and cation exchange chromatography were used to examine degradation of treated and untreated soybean meal protein fractions by bacteroides amylophilus h18(1), bacteroides ruminicola b(1)4, pepsin, trypsin, and intraruminally. soybean meal treatments consisted of 30% vol/vol isopropanol, 40% propanol, or 50% ethanol at 22 degrees c or 70% ethanol at 80 degrees c. water-soluble protein fractions were applied to a hydroxylapatite column and eluted with a d ...	1988	3141489
gene-specific differences in the aflatoxin b1 adduction of chicken erythrocyte chromatin.	mature and immature chicken erythrocyte nuclei were treated with activated aflatoxin b1 (2,3-dichloroaflatoxin b1), producing covalently bound dna adducts. this reaction produces alkali-labile sites in the dna which can be identified by using a variation of the maxam-gilbert sequencing procedure. we determined the aflatoxin b1 accessibility of defined regions of the erythroid genome by using different specific probes and monitoring the disappearance of similar-sized fragments generated by restri ...	1988	3142684
effect of phenolic monomers on the growth and beta-glucosidase activity of bacteroides ruminicola and on the carboxymethylcellulase, beta-glucosidase, and xylanase activities of bacteroides succinogenes.	trans-p-coumaric acid inhibited the growth of bacteroides ruminicola on both cellobiose and glucose, while trans-ferulic acid and vanillin retarded growth. the phenolic monomers varied in their potential to inhibit the bacteroides succinogenes beta-glucosidase, carboxymethylcellulase, and xylanase, with p-coumaric acid being the most inhibitory. the b. ruminicola beta-glucosidase was inhibited less than 10% by all three compounds.	1988	3146948
scanning electron microscopy of the substructure of smear layers in human dentine.	smear-layer debris was sonicated from dentine surfaces and trapped on microfilters for microscopy, which showed that the layers are composed, in part, of aggregates of globular subunits approx. 0.05-0.1 micron in diameter. such globules were also seen in smear layers in situ and on fractured dentine surfaces. the composition of smear layers may reflect the substructure of dentine matrix.	1988	3165261
esterase activities in butyrivibrio fibrisolvens strains.	thirty strains of butyrivibrio fibrisolvens isolated in diverse geographical locations were examined for esterase activity by using naphthyl esters of acetate, butyrate, caprylate, laurate, and palmitate. all strains possessed some esterase activity, and high levels of activity were observed with strains 49, h17c, s2, actf2, and lm8/1b. esterase activity also was detected in other ruminal bacteria (bacteroides ruminicola, selenomonas ruminantium, ruminobacter amylophilus, and streptococcus bovis ...	1988	3178205
effects of plasma ochratoxin a and luminal ph on the jejunal absorption of ochratoxin a in rats.	the effects of iv injection of ochratoxin a (oa) on its absorption from the jejunum in rats were studied in vivo to demonstrate the jejunal absorption of oa in relation to the oa level in blood plasma. in addition the effects of the ph of the medium on oa uptake by the everted jejunum of the rat were studied in vitro to assess the contribution made by transfer of the non-ionized form of oa to the jejunal absorption of the toxin. the in vivo study showed that oa was absorbed from the jejunum even ...	1988	3209137
use of a unique gene sequence as a probe to enumerate a strain of bacteroides ruminicola introduced into the rumen.	cloned fragments of genomic dna from the ruminal anaerobe bacteroides ruminicola subsp. brevis b14 were isolated and used as hybridization probes to identify closely related bacterial species. one dna fragment unique to strain b14 was tested to determine its sensitivity in detecting homologous sequences among total ruminal microbial dna. in a dna titration experiment, the probe was capable of detecting strain b14 sequences in vitro down to 0.1% of the total bacterial dna present in a hybridizati ...	1988	3355138
differentiation of ruminal bacterial species by enzyme-linked immunosorbent assay using egg yolk antibodies from immunized chicken hens.	cross-reactivity among four species of ruminal bacteria was examined by using egg yolk antibodies from immunized leghorn laying hens and an enzyme-linked-immunosorbent assay. the effects of the four species on the hens were compared on various days postimmunization. hens injected with the same bacterial species had similar apparent antibody levels over the entire postimmunization period, but only bacteroides ruminicola b1(4) and selenomonas ruminantium d antigens elicited early increases in appa ...	1988	3355145
enrichment and isolation of a ruminal bacterium with a very high specific activity of ammonia production.	when mixed ruminal bacteria were inoculated into semicontinuous cultures (25% transfer every other day) containing lactate, dulcitol, pectin, or xylose and trypticase (1 g/liter) as the sole nitrogen source, the specific activity of ammonia production increased. the greatest enrichment was observed with lactate and xylose, and in these cases the specific rate of ammonia production was eightfold higher than that of the ruminal fluid control (approximately 35 nmol of ammonia per mg of protein per ...	1988	3377500
satellite cells in the regenerated and regrafted skeletal muscles of rats.	soleus (sol) muscles were grafted into extensor digitorum longus (edl) muscle beds (edl-first-graft). sixty days later, some mature edl-first-grafts were regrafted into their own beds (edl-second-grafts). fully regenerated muscle fibers and satellite cells were observed in both types of mature grafts. the ratios of satellite cell nuclei per total nuclei (myonuclei and satellite cell nuclei) were 4.81 +/- 0.47% for edl-2nd graft, 4.26 +/- 0.51% for edl-1st-graft, 4.30 +/- 0.33% for control sol, a ...	1988	3396655
the effect of tissue culture agar on chromosome breakage, sister-chromatid exchanges and clonogenicity in human cells.	to investigate the cytogenetic effects of electromagnetic fields, a system containing an agar gel was developed to support the growth of various human cell types (peripheral lymphocytes, lymphoblasts, and fibroblasts). when compared to alioquots of identical cells, grown in plastic culture vessels, statistically significant increases in the frequencies of chromosome breakage, sister-chromatid exchange and decreased cloning efficiency were observed in those cells cultured in the agar. these resul ...	1988	3398870
amylolytic activity of selected species of ruminal bacteria.	a variety of species of ruminal bacteria were screened for the ability to grow in starch-containing medium and produce amylase. of those tested, the highest levels of amylase were produced by streptococcus bovis jb1 and ruminobacter amylophilus h18. other strains that grew well on starch and produced amylase included butyrivibrio fibrisolvens a38 and 49 and bacteroides ruminicola 23 and b14. varying the carbohydrate source provided for growth resulted in changes in the growth rate and level of a ...	1988	2454075
[sebastian kneipp, his life and his work].		1988	202779
poly (adp-ribose) polymerase activity in nucleotide permeable cells.		1988	202502
x-ray absorption edge studies on cyanide-bound cytochrome c oxidase.		1988	202486
central sites of action of gastrointestinal drugs.		1988	202539
[anaerobic bacteria in intracranial suppuration].	in four cases of intracranial suppurative disease, studied in the laboratory of anaerobic bacteria of institute pasteur of algiers, three cases are caused by anaerobic bacteria and one remain sterile. two of them are empyema with unknown origin and the others are brain abscess with bilateral chronic ear infection to one. in the three cases, gram negative anaerobic bacilli were grown in culture: bacteroides asaccharolyticus, bacteroides vulgatus and bacteroides ruminicola brevis. in two cases, th ...	1989	2489404