Publications

[isolation of preparations of type b monoamine oxidase from liver mitochondria].		1977	193582
[human genetics: a task for the public health service? (author's transl)].		1977	143637
decline of maternal antibodies to plague in norway rats.	the decline of maternal antibodies to the fraciton i antigen of yersinia pestis was investigated in newly weaned rattus norvegicus obtained from dams vaccinated with strain ev76(51f) of y. pestis. iha titre decreased by 50% each 7-3 days and cf titre declined 50% each 10-0 days in young rats. an analysis of available data indicated that maternal iha and cf antibodies could persist to 3 months of age. therefore, positive serologic reactions in young r. norvegicus, detected in the course of serolo ...	1977	264498
nonrandom chromosome rearrangements in 27 cases of human myeloid leukemia.	the r-banding pattern of the chromosomes of 31 patients hospitalized in the hematologic clinic for myeloid leukemia were studied before chemotherapy. this analysis permitted identification of one unusual 3-chromosome rearrangement t(3;9;22) in addition to 25 classic forms of (22q-;9q+) translocation accompanied by the specific ph' chromosome in chronic granulocytic leukemia patients, independent of the blastic course of the disease. during blastic crisis observed in 6 patients, extra 8 and 10 ch ...	1977	271632
genetic and environmental factors in the etiology of dental disease in the aleut.		1977	273033
the effects of additional flora on the response of salmonella mutants lodged in the gastrointestinal tract.	a histidine auxotroph of salmonella typhimurium, strain ta1538, will lodge for several months in the gastrointestinal tract of otherwise germ-free rats and of rats additionally associated with bacteria characteristic of the normal flora such as lactobacillus plantarum and bacteroides vulgatus. in the presence of the additional flora, the concentration of strain ta1538 is diminished in the stomach but not in the lower gastrointestinal tract or in the feces. following the ingestion of 2-nitrofluor ...	1977	318921
superoxide dismutase in bacteroides fragilis and related bacteroides species.	superoxide dismutase (sod) activity was demonstrated in cell-free extracts of bacteroides fragilis, bacteroides vulgatus, bacteroides distasonis, bacteroides ovatus, and bacteroides thetaiotaomicron. the strains were grown under anaerobic conditions in trypticase soy broth, and the specific activity of sod in the extracts was, in most strains, higher than in cell-free extracts of escherichia coli b grown under anaerobic conditions. isoelectric focusing of the extracts in polyacrylamide gel demon ...	1977	332713
[in vitro susceptibility of bacteroidaceae to cefoxitin and cephalothin (author's transl)].	the activity of the new cephamycin c derivatives cefoxitin against 102 clinical isolates of bacteroidaceae (95 bacteroides strains and 7 fusobacterium-sphaerophorus cultures) was determined by tube dilution and standardized agar diffusion tests and compared to that of cephalothin. cefoxitin was more active than cephalothin against 56 bacteroides fragilis strains, 19 bacteroides thetaiota-omicron strains and two bacteroides splanchnicus strains. the difference seemed less pronounced with five bac ...	1977	856727
demonstration of thy-1 antigen on pluripotent hemopoietic stem cells in the rat.	three approaches were used to demonstrate the presence of thy-1 antigen on the surface of pluripotent hemopoietic stem cells in the rat. in the first, stem cells from fetal liver, neonatal spleen, and adult bone marrow were prevented from forming hemopoietic colonies in the spleens of irradiated recipients spleen (colony-forming unit assay) by incubation with antibodies to thy-1 antigen. highly specific rabbit heteroantiserum to purified rat brain thy-1 antigen and mouse alloantisera to thy-1.1- ...	1978	309914
swine dysentery: studies of gnotobiotic pigs inoculated with treponema hyodysenteriae, bacteroides vulgatus, and fusobacterium necrophorum.	transmission experiments were carried out in gnotobiotic pigs to determine whether lesions typical of swine dysentery could be produced by oral inoculation of treponema hyodysenteriae in combination with bacteroides vulgatus or fusobacterium necrophorum, or both. each of the organisms had been isolated from swine with early lesions of the disease. lesions were not found in 6 pigs inoculated with t hyodysenteriae alone, in 4 pigs given f necrophorum and t hyodysenteriae, or in 4 pigs given b vulg ...	1978	624669
factors affecting production of catalase by bacteroides.	several variables affected the production of catalase by members of the "bacteroides fragilis group" of anaerobic bacteria. both media yielded higher catalase levels than the respective agar media. addition of hemin to media after autoclave sterilization, rather than before, significantly increased production of catalase. both of these variables could be related to the available hemin concentration present in the medium being tested. significantly higher amounts of hemin were required for catal ...	1978	730827
ovarian involvement by cytomegalovirus.		1978	204571
electron-microscopic identification of axonal initial segments belonging to interneurons in the dorsal lateral geniculate nucleus of the monkey.		1978	98733
[some aspects relating to the aflatoxin generation during the self-heating of cereals].	in a stored batch of grain which was already affected by mould-formation tests were carried out with the known aflatoxin producer aspergillus flavus. a significantly lower aflatoxin production ensued if the mould growth was not connected with self-heating of the stored product. however, in conformity with increasing mould formation the germinating power was adversely affected and the significant signs (fatty acid number, reductive and none-reductive sugars) were influenced in the grain, irreleve ...	1978	98933
[intratypical antigenic differentiation of poliovirus strains circulating in mali by means of cross-adsorbed sera].	the intratypic antigenic differentiation of poliovirus strains isolated from sick or healthy children in mali in 1975-1976 was performed. the method is based on the use of cross-adsorbed sera in the neutralization tests in which the virus is titrated by the plaque procedure or by the cytopathic effect. all the examined strains of serologic types i, ii, and iii were distinguished by the antigenic structure from the vaccine strains and were classified as "wild". quantitative differences in the ant ...	1978	86237
the use of trypan blue for counterstaining in the sepharose bead immunofluorescence test. the application of the test for the demonstration of primate retrovirus-specific antibodies and antigens.	the sepharose bead immunoflurorescence test was performed by counterstaining the beads with trypan blue. this results in a red staining of negative beads which allows an easy distinction from positive green-fluorescent beads. sepharose beads conjugated with viral proteins or antiviral antibodies were used to demonstrate mason-pfizer monkey virus (mpmw)- and simian sarcoma virus (ssv) - specific antigens or antibodies. the test shows a high sensitivity and specificity and needs a small amount of ...	1978	86253
antibodies detectable by counterimmunoelectrophoresis against bacteroides antigens in serum of patients with inflammatory bowel disease.	heat-extracted antigens from seven species of bacteroides were used in passive hemagglutination and counterimmunoelectrophoretic tests. sera from 87 normal persons (group i) and 15 patients with ulcerative colitis (group ii) were of low and equal reactivity in passive hemagglutination tests; all positive tests were eliminated by 2-mercaptoethanol reduction of the sera. when these same sera were tested by counterimmunoelectrophoresis with six of the bacteroides antigens, no significant difference ...	1979	88458
the pathophysiology of asthma.	because postmortem studies of humans provide little information on the initial pathophysiologic events in asthma, animal models have been developed. recently the ascaris-allergic rhesus monkey has provided an opportunity to examine the onset of pathophysiologic changes following challenge and to correlate them with airway structure. these studies have suggested that the initial interaction between antigen and mast cells may occur in the bronchial lumen or in the epithelium superficial to the tig ...	1979	89006
histamine-releasing effect of a corticotrophin derivative. ii. mechanism of action of histamine release by c 44 680-ba, compared with that of cpd. 48/80, dextran and triton.	the mechanism of the histamine-liberating action of the synthetic polypeptide c 44 680-ba, an alkyl-prolyl derivative of beta 1-19 corticotrophin, was investigated and compared with those of compound 48/80, dextran, melittin and triton x-100. it was found that the release of histamine from rat peritoneal cells induced by the polypeptide is dependent on temperature, ph, calcium ions and energy-providing processes. in regard to these criteria, the mode of action of this histamine liberator resembl ...	1979	89804
distribution of myosin isoenzymes among skeletal muscle fiber types.	using an immunocytochemical approach, we have demonstrated a preferential distribution of myosin isoenzymes with respect to the pattern of fiber types in skeletal muscles of the rat. in an earlier study, we had shown that fluorescein-labeled antibody against "white" myosin from the chicken pectoralis stained all the white, intermediate and about half the red fibers of the rat diaphragm, a fast-twitch muscle (gauthier and lowey, 1977). we have now extended this study to include antibodies prepare ...	1979	90047
the use of avidin-biotin interaction in immunoenzymatic techniques.	biotin was covalently attached to antibodies, antigens and enzymes, and the effects of this labeling on the antigen and antibody binding capacity and on enzymatic activity were tested. based on avidin-biotin interaction, the labeled proteins were used in quantitative enzyme-immunoassay and enzyme-immunohistochemical staining procedures. two procedures were developed. in the first procedure, named the bridged avidin-biotin (brab) technique four steps were used sequentially in order to quantify or ...	1979	90074
polyribonucleotide inhibition of ribonucleic acid directed deoxyribonucleic acid polymerase of mouse mammary tumor (type b) virus and simian sarcoma (type c) virus.		1979	90331
the expression of a human b-lymphocyte antigen and surface membrane immunoglobulin by lymphoid cells from patients with lymphocytic lymphoma, multiple myeloma and benign monoclonal gammapathy.		1979	90449
the intra-nucleolar localization of amplified rdna in xenopus laevis oocytes.	a fluorescent feulgen-stain was adapted in order to demonstrate dna-containing structures inside the amplified nucleoli of xenopus laevis. at all stages of oogenesis this method reveals granules or complex structures of dna in each nucleolus. the micronucleoli which do not stain with this method and which do not reveal an internal structure in low molarity saline, unlike real nucleoli are considered as nucleolus-like bodies. the dna-containing structures in the nucleoli can be composed of one or ...	1979	90588
antigens in penicillin allergy. iv. induction of ige antibody response in mice after daily treatment with contaminated but not with pure penicillin.		1979	90657
characterization of a type-c virus produced by co-cultures of human leukemic bone-marrow and fetal canine thymus cells.	the putative human helper virus ska-21/a204v, isolated by nooter et al. in 1977 from human leukemic bone-marrow cells following co-culture with normal fetal canine thymus cells, cf2th, has been characterized with respect to its major viral core protein, reverse transcriptase, and nucleic acid sequences. the results of these analyses show that this virus is not distinguishable from the woolly monkey type-c virus, ssav-1, by the techniques employed.	1979	90662
purification of a new high activity form of glucose-6-phosphate dehydrogenase from rat liver and the effect of enzyme inactivation on its immunochemical reactivity.	a new form of cytoplasmic glucose-6-phosphate dehydrogenase (e.c.1.1.1.49) was purified from rat liver by protamine sulfate precipitation, ammonium sulfate fractionation, ion exchange chromatography with diethylaminoethyl cellulose, and affinity chromatography with cibacron blue agarose and nadp agarose. this form of the enzyme has a specific activity of over 600 units/mg of protein and gives essentially a single band by polyacrylamide gel electrophoresis. the form of the enzyme isolated by this ...	1979	90677
radioprotection of mouse intestine by inhibitors of cyclic amp phosphodiesterase.		1979	91600
binding of purified, soluble major histocompatibility complex polypeptide chains onto isolated t-cell receptors. i. reactivity against allo- and self-determinants.	in this study, we tried to get information about the fine antigen-binding ability of purified, soluble, idiotype-positive t-cell receptor molecules. lewis anti-da t-cell receptors were purified from normal lewis serum by the use of anti-idiotypic immunosorbent and sodium dodecyl sulfate-polyacrylamide gel, and were coupled to cyanogen bromide-activated sepharose 4b. in parallel, lewis anti-da, lewis anti-bn, and da anti-lewis alloantibody immunosorbents were prepared. the major ag-b chain (44,00 ...	1979	91653
trace polypeptides in cellular extracts and human body fluids detected by two-dimensional electrophoresis and a highly sensitive silver stain.	development of a highly sensitive silver stain permits the characterization of trace cellular and body fluid proteins separated by the two-dimensional electrophoresis technique of o'farrell. many of the proteins detected by the silver stain in urine, spinal fluid, amniotic fluid, and cells were undetected with the widely used coomassie blue stain. trace polypeptides observed in escherichia coli cell lysates with this silver stain could be detected previously only by growth in radioactive precurs ...	1979	92027
[isolation of treponemas from the colon of pigs with clinical dysentery].	optimal culture conditions in artificial nutritive media were determined for a defined avirulent strain of treponema hyodysenteriae and for four field strains of treponemas in pigs with clinical dysentery. the treponemas were isolated with the use of milliporous filters with pores of 0.3 micrometer in diameter, which were located on the surface of blood agar. no significant difference in the influence of equine, bovine or sheep blood on the growth of treponemas was determined. the commercial amo ...	1979	105448
multiple sclerosis: immunochemical studies on the demyelinating serum factor.	sera from patients with multiple sclerosis (ms) frequently produce demyelination of central nervous system tissue cultures. the nature of the factors responsible for demyelination is not as yet clearly established. however, several authors previously reported, in in vivo and in vitro models, demyelinating activity in igg fractions isolated from sera and cerebrospinal fluid of ms patients14,42,48. we found the demyelinating activity of ms sera to be extremely labile to conventional biochemical tr ...	1979	105780
betamethasone and the rhesus fetus: multisystemic effects.	in this controlled study of betamethasone administration to pregnant rhesus monkeys, using dosages per gram of fetal body weight similar to those reported in several human clinical studies, the most significant fetal pulmonary changes observed were increases in maximum lung volumes. the fact that comparable increases in peak volumes were demonstrated on saline filling supports our contention that these changes are related primarily to lung structural alterations rather than surfactant effects. a ...	1979	106730
effects of trh and ergocryptine on mammary arterial and venous concentrations of growth hormone in lactating cows.		1979	107518
[new species of microsporidians (microsporidia, nosematidae) from horsefly larvae in the ukraine].	a new species of microsporidians, stempellia lairdi sp. n., was found in larvae of tabanus autumnalis l. from ukraine. it affects the muscular tissue and haemolymph of horse fly larvae. spores of the species are oval, egg-shaped, 1.8--7.6 x 1.2--3.8 mu in size. the species is pathogenic and causes the mortality of infected larvae.	1979	108652
protein structure refinement: streptomyces griseus serine protease a at 1.8 a resolution.		1979	119870
neonatal low-level lead exposure in monkeys: locomotor activity, schedule-controlled behavior, and the effects of amphetamine.		1979	120039
characterization of a murine myeloma (mopc-315) c-type virus by nucleic acid hybridization.		1979	219600
activity of semisynthetic penicillins and synergism with mecillinam against bacteroides species.	the minimal inhibitory concentrations (mic) of six penicillins (ampicillin, carbenicillin, ticarcillin, piperacillin, mezlocillin, and bay k 4999) against 29 clinical isolates of bacteriodes spp. (including bacteroides fragilis, bacteroides thetaiotaomicron, and bacteroides vulgatus) were determined by an agar dilution method. bay k 4999 was most active, followed in descending order by ampicillin, piperacillin, mezlocillin, ticarcillin, and carbenicillin. mecillinam, a 6 beta-amidino-penicillani ...	1979	228593
comparison of primary and secondary stimulation of male rats by estradiol in terms of prolactin synthesis and mrna accumulation in the pituitary.	male rats received acute or chronic primary or acute secondary stimulation with estradiol, and the effects on pituitary prolactin synthesis and its mrna accumulation were examined. prolactin synthesis was determined by the in vitro incorporation of [(3)h]leucine into prolactin over a period of 1 hr. prolactin mrna was measured both by cell-free translation in a nuclease-treated rabbit reticulocyte lysate and by hybridization to the complementary dna. the latter two methods gave similar results u ...	1979	284404
use of ganglioside affinity filters to identify toxigenic strains of clostridium botulinum types c and d.	clostridium botulinum neurotoxin is synthesized by toxic clones grown anaerobically on ganglioside affinity filters. the toxin binds to the filters and is detected by reaction with 125i-immunoglobulin g from type-specific antitoxin. toxin spots from culture filtrates were similarly identified. the c. botulinum type c and d strains were selected for developing this affinity filter assay because synthesis of the c1 and d toxins is bacteriophage dependent. toxigenic clones were distinguished from p ...	1979	387601
incidence and pathological features of spontaneous tumors in athymic nude mice.	systematic observation of 1141 nude mice (swiss background [corrected] strain) that received human tumor transplants revealed 24 spontaneous tumors, 18 of lymphoreticular origin and 6 pulmonary adenomas. spontaneous tumors were seen at an average age of 9.1 months, and 22 of the tumors were seen only in that fraction of our group (324 mice) surviving for 5 months or more (22 of 324 x 100 = 6.8%). transplantation of these tumors to other nude mice was successful in three of five cases. mice trans ...	1979	427772
[bacteriological and clinical studies on metronidazole in bacteroides fragilis infections (author's transl)].	the antimicrobial activity of 24 bacteroides fragilis, 13 bacteroides thetaiotaomicron and 5 bacteroides vulgatus, all of which were recent clinical isolates, was determined by the agar dilution technique. these three species had similar susceptibility patterns, but the b. thetaiotaomicron strains were generally less susceptible than the b. fragilis strains. clindamycin was very active against 81% of the bacteroides strains at 3.1 mcg/ml of less while 21% of the bacteroides strains had mics of 1 ...	1979	439390
[importance of bilirubinuria in the diagnosis of viral hepatitis].		1979	452553
studies on selective induction of lysosomal enzyme activities in mouse peritoneal macrophages.		1979	522087
pathogenic synergism between treponema hyodysenteriae and other selected anaerobes in gnotobiotic pigs.	gnotobiotic pigs were orally exposed to various anaerobes at 6 to 9 days of age and similarly inoculated with treponema hyodysenteriae b204 3 to 6 days later. watery diarrhea and fecal excretion of large quantities of mucus and some fibrin clots were observed 4 to 20 days after inoculation with b204 if other anaerobes were present. colonic lesions characteristic of swine dysentery were observed when b204 was present with fusobacterium necrophorum, three strains of bacteroides vulgatus, a clostri ...	1979	528047
synthesis of alpha-ketoglutarate by reductive carboxylation of succinate in veillonella, selenomonas, and bacteriodes species.	evidence for reductive carboxylation of succinate to synthesize alpha-ketoglutarate was sought in anaerobic heterotrophs from the rumen and from other anaerobic habitats. cultures were grown in media containing unlabeled energy substrates plus [14c]succinate, and synthesis of cellular glutamate with a much higher specific activity than that of cellular asparate was taken as evidence for alpha-ketoglutarate synthase activity. our results indicate alpha-ketoglutarate synthase functions in selenomo ...	1979	533772
[new capitonnage technic in pulmonary echinococcectomy].		1979	352846
a history of porcelain in dentistry.		1980	371713
informed consent--defense orthodontics.		1980	291345
specificity of immunoglobulin m antibodies in normal human serum that participate in opsonophagocytosis and intracellular killing of bacteroides fragilis and bacteroides thetaiotaomicron by by human polymorphonuclear leukocytes.	studies were performed to determine the specificity of immunoglobulin m (igm) antibodies in normal human serum that participate in opsonophagocytosis and intracellular killing of bacteroides fragilis 1365 and bacteroides thetaiotaomicron 1343 by human polymorphonuclear leukocytes. purified normal human igm was adsorbed with washed heat-killed cells of the homologous strains and heterologous strains of b. fragilis, b. thetaiotaomicron, bacteroides vulgatus, bacteroides distasonis, and bacteroides ...	1980	6160104
specific strains of bacteroides species in human fecal flora as measured by deoxyribonucleic acid homology.	membrane competition homology experiments were used to compare bacteroides uniformis and bacteroides vulgatus isolates obtained from fecal samples from different individuals and isolates obtained from fecal samples of single individuals. isolates of b. uniformis, when isolated from different individuals, had interstrain deoxyribonucleic acid homology values that ranged from 63 to 95%, with most of the values being in the 70 to 85% range. when isolates obtained from a single individual were compa ...	1980	6990866
effect of secreted bacteroides proteases on human intestinal brush border hydrolases.	selected bacteroides species secreted various amounts of protease and glycosidase into their growth medium. bacteroides vulgatus, distasonis, and ovatus secreted the most (31-60% of total). the secreted protease was similar in action to the protease within the organism, in that it had a broad ph optimum of 6-9, a k(m app.) for casein of 0.1 mum, and was inhibited by benzamidine, phenylmethylsulfonyl fluoride, diisopropylfluorophosphate (difp), and by an elastase inhibitor, ac(ala)(3)alach(2)cl. ...	1980	6995483
enkephalin-containing neurons visualized in spinal cord cell cultures.	neuronal cells, axons, and terminals containing immunoreactive enkephalin have been visualized in cultures of dissociated fetal spinal cord. these cultures may provide a valuable system in which to explore the effects of chronic drug treatment on the physiology of enkephalin-containing cells and their interactions with other cells.	1981	351811
lymphocytotoxic and microbial antibodies in crohn's disease and matched controls.	patients with crohn's disease and age, sex and seasonally matched healthy controls were studied for cold lymphocytotoxic and a variety of microbial antibodies. lymphocytotoxic antibody titers were increased significantly in the crohn's patients compared to controls, but did not correlate with any of the microbial titers. antibodies to a pseudomonas-like bacterium and bacteroides vulgatus were also increased in the patients, but titers to chlamydia trachomatis, rotavirus and norwalk virus were no ...	1981	6275790
in vitro activity of mk0787 (n-formimidoyl thienamycin) and other beta-lactam compounds against bacteroides spp.	the susceptibilities of 82 strains of the bacteroides fragilis group to eight beta-lactam compounds, lincomycin, and metronidazole were determined by using an agar dilution technique. mk0787 (n-formimidoyl thienamycin) was the most active compound, inhibiting all strains at a concentration of 1 microgram/ml. metronidazole was the only other drug of similar activity. of the beta-lactam compounds, cefoxitin and mk0787 showed uniform activity against all species, whereas most other compounds were r ...	1981	6282191
production of lesions in gnotobiotic mice by inoculation with treponema hyodysenteriae.	treponema hyodysenteriae was established in the ceca of gnotobiotic mice in the absence of other organisms. superficial mucosal lesions characteristic of swine dysentery were present in the ceca of mice inoculated with t. hyodysenteriae in combination with viable bacteroides vulgatus. deep crypt necrosis was detected in the ceca of mice inoculated with t. hyodysenteriae alone.	1981	7216455
production of experimental ulcerative colitis in gnotobiotic guinea pigs with simplified microflora.	conventional guinea pigs provided with a solution of 5% (wt/vol) degraded carrageenan as the sole source of oral fluids developed ulcerations of their ceca and large intestines within 30 days. similar lesions were not detected in germfree guinea pigs treated in an identical manner, suggesting that an intestinal microflora was necessary for development of intestinal lesions. to simplify the bacterial flora required for production of cecal ulcerations, 10 pools consisting of 10 bacterial strains e ...	1981	7216487
the cell-surface antigens of bacteroides vulgatus.	the cell surface of bacteroides vulgatus was examined by electron microscopy. the outer membrane complex was removed by edta and mild sonication and the antigens of this complex were characterized by enzyme-linked immunosorbent assay and crossed immunoelectrophoresis. the species-specific antigen was identified and was shown to be the major outer membrane protein with a molecular weight of 100 000.	1981	7334331
[clinical pharmacokinetics of azabutyron, a new short-term action neuroleptic].	pharmacokinetics of azabutyron--a new soviet-made neuroleptic was studied clinically without anesthesia and also during surgery against the background of deep and surface fluorothan anesthesia in conjunction with artificial nitrous oxide and oxygen ventilation of the lungs (2:1). after intravenous administration of the drug in a dose of 4 mg/kg its maximum concentration (about 8 gamma/ml) was recorded in 5 minutes and the amount eliminated during 2 hours of observation comprised 1--2 per cent of ...	1981	350611
the direct immunofluorescence in the presumptive identification of bacteroides species.	two kits containing fluorescein labeled-polyvalent antisera to bacteroides fragilis, bacteroides distasonis, bacteroides ovatus, bacteroides thetaiotaomicron, bacteroides vulgatus (fluoretec-f) and to bacteroides melaninogenicus subsp. melaninogenicus and intermedius and to bacteroides asaccharolyticus (non-oral strains) (fluoretec-m) were evaluated for the identification of the respective organisms in bacterial cultures and clinical specimens. in both cases immunofluorescent tests were compared ...	1982	6126973
the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract.	the development of the bacterial flora of neonates with congenital abnormalities of the gastro-intestinal tract was studied in 31 infants during the first 10 days of life. specimens were collected from the umbilicus, mouth and gastro-intestinal tract on the pre-operative day, at operation and on post-operative days 1, 2, 3, 5, 7 and 10. bacteria were isolated semi-quantitatively on a variety of plain and selective media and identified by conventional methods.staphylococcus albus was the predomin ...	1982	7057028
properties of a new penicillinase type produced by bacteroides fragilis.	a highly penicillin-resistant strain of bacteroides fragilis, strain gn11499, was found among 80 clinical isolates of the b.fragilis group and appears to produce a new type of penicillinase. penicillinase activity was detected in crude extracts and had a specific activity of 0.25 u/mg of protein. about 20% of the enzyme was released into the surrounding medium during growth. the enzyme hydrolyzed ampicillin and cloxacillin more rapidly than it did penicillin g, carbenicillin, and cephaloridine. ...	1982	6983857
quantitative variability in requirements for opsonization of strains within the bacteroides fragilis group.	requirements for opsonization of various strains within the species bacteroides fragilis, bacteroides thetaiotaomicron, bacteroides vulgatus, and bacteroides distasonis were investigated. the minimal concentration of normal human serum that facilitated maximal uptake by human peripheral leukocytes of 14c-labeled strains ranged from 5% to 80%. heated (56 c at 30 min) serum produced less uptake than untreated serum, suggesting that complement was required for opsonization. using human serum deplet ...	1983	6631060
enhancement of experimental ulcerative colitis by immunization with bacteroides vulgatus.	previous studies with the guinea pig model for ulcerative colitis have shown that the inducing agent, carrageenan, does not provoke ulcerations in germfree guinea pigs, but animals monoassociated with bacteroides vulgatus develop cecal ulcerations when fed carrageenan. in an effort to define the role of b. vulgatus in this model system, conventional guinea pigs were immunized with b. vulgatus before carrageenan treatment. immunized animals developed both circulating antibody and positive skin te ...	1983	6642651
infectivity of organisms recovered from polymicrobial abscesses.	the ability to cause subcutaneous abscesses in mice was used to identify the pathogens among the bacteria recovered from 13 clinical abscesses. a total of 35 isolates (30 anaerobes and 5 aerobes), 16 of which were encapsulated, were recovered from these abscesses. encapsulated organisms included eight bacteroides spp. (three bacteroides asaccharolyticus and one strain each of bacteroides oralis, bacteroides intermedius, bacteroides biacutus, bacteroides vulgatus, and bacteroides ruminicola subsp ...	1983	6642674
bacteria in enteric lesions of cattle.	thirty-nine species of bacteria were isolated from or demonstrated in the abomasal and small and large intestinal mucosa of 23 adult cattle and 41 calves and identified. the bacteria isolated were related to the gross and microscopical lesions. campylobacters, clostridium perfringens type a, c sordellii, actinobacillus lignieresii, fusobacterium necrophorum, mycobacterium paratuberculosis, aeromonas hydrophila and escherichia coli were all associated with specific lesions. the relationship of ot ...	1983	6829137
use of randomly cloned dna fragments for identification of bacteroides thetaiotaomicron.	randomly cloned fragments of dna from bacteroides thetaiotaomicron were used as hybridization probes for differentiation of b. thetaiotaomicron from closely related bacteroides species. hindiii digestion fragments of dna from b. thetaiotaomicron (type strain) were inserted into plasmid pbr322 and labeled with [alpha-32p]dctp by nick translation. these labeled plasmids were screened for hybridization to hindiii digests of chromosomal dna from type strains of the following human colonic bacteroide ...	1983	6833179
demonstration of bacteroides capsules by light microscopy and ultrastructural cytochemistry.	forty-six anaerobic gram-negative bacilli, including 26 members of the bacteroides fragilis group (bfg), were examined for capsules by the india ink technic. thirty-five were encapsulated, including all the bfg strains. as a follow-up, seven of these isolates and two previously studied reference strains (b. fragilis atcc 23745 and bacteroides vulgatus atcc 8482) were examined for capsules by ultrastructural cytochemistry. using the periodic acid thiocarbohydrazide silver proteinate (patcsp) meth ...	1983	6837522
metabolism of mercapturic acid-pathway metabolites of 2-chloro-n-isopropylacetanilide (propachlor) by gastrointestinal bacteria.	1. mercapturic acid pathway metabolites of propachlor, labelled with 14c, were incubated with pig caecal contents, small and large intestinal contents, and pure cultures of gastrointestinal bacteria. 2. the glutathione, cysteine, n-acetylcysteine, and the s-oxide of the n-acetylcysteine conjugates of propachlor (2-chloro-n-isopropylacetanilide) were each metabolized by mixed pig caecal micro-organisms to 2-mercapto-n-isopropylacetanilide. 3. the extent of formation of 2-mercapto-n-isopropylaceta ...	1983	6880239
bactericidal activity of a granule extract from human polymorphonuclear leukocytes against bacteroides species.	the microbicidal activity of an acetate extract of human polymorphonuclear leukocyte granules was tested against bacteroides fragilis, bacteroides vulgatus, bacteroides distasonis and bacteroides thetaiotaomicron. all strains tested were killed by the extract, and there were no significant differences between the different bacteroides species.	1983	6885166
characterization of cephalosporinases from bacteroides fragilis, bacteroides thetaiotaomicron and bacteroides vulgatus.	the susceptibility of 80 bacteroides fragilis group strains isolated from clinical specimens to beta-lactam antibiotics was investigated by agar dilution method. twenty strains showed high resistance to the antibiotics. the resistance level of the isolates to cephaloridine was related to the amount of beta-lactamase activity (cephalosporinase; csase) produced. b. fragilis gn-11477, b. thetaiotaomicron gn11478 and b. vulgatus gn11479 were selected from among the csase producing strains, and the e ...	1983	6332105
open study of ceftazidime in serious infections due to multiply-resistant bacteria.	we treated 37 infections in 32 patients who had one or more significant underlying diseases. most of the responsible micro-organisms were multiresistant, but were initially sensitive to ceftazidime. they included 16 pseudomonas spp., 10 serratia spp., 6 klebsiella spp., 7 other enterobacteriaceae, 3 staphylococcus spp., 3 strict anaerobes and 1 acinetobacter anitratus. indications for ceftazidime encompassed a broad spectrum of diseases: 10 complicated uti, 13 skeletal and soft tissue infections ...	1983	6352619
alpha-ketoglutarate metabolism by cytochrome-containing anaerobes.	during growth in the presence of tracer amounts of exogenously supplied alpha-keto[1-14c]glutarate (akg) or alpha-keto [5-14c]glutarate, cytochrome-containing bacteroides fragilis strain 2044 and bacteroides vulgatus strain 8482 incorporated extremely small amounts of radioactivity into cell macromolecules and protoheme. under identical conditions, bacteroides "l" strain 7cm and bacteroides buccae strain j1 incorporated substantial label from [5-14c]akg, but not [1-14c]akg, into cellular macromo ...	1983	6413047
dentine dysplasia with sclerotic bone and skeletal anomalies inherited as an autosomal dominant trait. a new syndrome.	patients with teeth showing all of the clinical, radiographic, and histologic features of radicular dentine dysplasia (type i) have been found to have dense sclerotic bone and skeletal anomalies of the wrists and hand bones. the association of these defects of teeth and bone was found to be transmitted as an autosomal dominant trait over four generations. review of theliterature failed to disclose a previous description of this disorder.	1983	264650
electrographic analysis of the sleep cycle in young depressed patients.	electrographic (eeg) patterns of nocturnal sleep were investigated in young psychiatric patients during unipolar depressive episodes. eeg-sleep data was recorded in 20 non-psychotic depressed patients all under 26 years old individually matched with a normal control group. all 20 subjects slept in the laboratory for 1-3 consecutive baseline nights from 12-8:00 a.m. during a subsequent extended condition 14 in each group were allowed to sleep ad-lib. although the mean total time asleep on baselin ...	1983	217458
patterns of alveolar bone loss in nigerian yorubas.	unusual patterns of alveolar bone loss in 33 nigerian yorubas with severe periodontal disease are described. the subjects, aged 15-50 yr represented 1.4% of a population of dental patients in western nigeria. periodontal pocketing and alveolar bone loss in the incisor and first molar regions, far in advance of other mouth areas, were the criteria for inclusion in this report. intra-oral radiographs showed advanced horizontal or crescentic bone resorption in incisor regions, and vertical or arc-l ...	1984	271137
naturally-occurring hemagglutinin in a tunicate halocynthia pyriformis.		1984	239759
distribution and effects of a defined six-member murine-derived microflora in gnotobiotic gerbils.	the gnotobiotic gerbil was selected as a model with which to study the effects of colonization with a defined microflora on organ morphology, histology, and selected blood biochemical parameters. gerbils were maintained germfree for 13 months but failed to reproduce, presumably because of the enlarged cecum. a colony of gnotobiotic gerbils that was associated with a bacterial flora consisting of lactobacillus brevis, streptococcus faecalis, staphylococcus epidermidis, bacteroides vulgatus, enter ...	1984	6426388
adoptive transfer of immune enhancement of experimental ulcerative colitis.	previous experiments with the carrageenan model for ulcerative colitis have shown that the inflammatory response in guinea pigs can be enhanced by immunization with and subsequent feeding of bacteroides vulgatus to experimental animals. the present studies showed that only certain strains of b. vulgatus are capable of provoking immune enhancement of ulcerative colitis. animals were fed carrageenan and various strains of viable b. vulgatus after immunization with a strain of b. vulgatus isolated ...	1984	6480115
the intestinal microflora of infants: composition of fecal flora in breast-fed and bottle-fed infants.	the fecal flora of 35 breast-fed and 35 bottle-fed babies was determined. bifidobacteria were the predominant fecal bacteria in both groups. conversely, the counts of most of the other bacteria, such as bacteroides, eubacteria, peptococci, veillonella, clostridia, enterobacteria, streptococci, and bacilli in the bottle-fed group were significantly higher than those in the breast-fed group. the frequencies of occurrence of lecithinase positive clostridia, clostridia-others, pseudomonas and bacill ...	1984	6513816
rapid glutamic acid decarboxylase test for identification of bacteroides and clostridium spp.	a rapid 4-h test for glutamic acid decarboxylase is described for the identification of certain anaerobic bacteria. the test substrate consisted of 1.0 g of l-glutamic acid, 0.3 ml of triton x-155, and 0.05 g of bromcresol green sodium salt in 1 liter of water. the substrate was dispensed in 0.5-ml amounts into test tubes, and a turbid suspension was made with the test organism. the test was then incubated aerobically at 35 degrees c for 4 h. the development of a blue color was considered positi ...	1984	6376535
activation of hageman factor by lipopolysaccharides of bacteroides fragilis, bacteroides vulgatus, and fusobacterium mortiferum.	preincubation of lipopolysaccharides (lps) of bacteroides fragilis, bacteroides vulgatus, and fusobacterium mortiferum with purified human hageman factor (hf) followed by addition of purified human prekallikrein resulted in the activation of hf, as measured by the generation of kallikrein using the tripeptide substrate n-alpha-benzoyl-l-proline-l-phenylalanine-l-arginine-4-nitroanilide. addition of purified hf, which had been preincubated with the lps, to hf-deficient plasma resulted in the acce ...	1984	6718941
susceptibility of intestinal anaerobes to new beta-lactam antibiotics.	in vitro susceptibility of 473 isolates of anaerobic bacteria derived from patients hospitalized for treatment of abdominal trauma was tested against third-generation cephalosporins--moxalactam, cefotaxime, ceftizoxime, cefmenoxime--and a penicillin--n-formimidoyl thienamycin--by serial agar dilution method in the anaerobic glove box. there were 208 bacteroides isolates including 80 bacteroides fragilis and 100 peptococci and peptostreptococci. the minimal inhibitory concentration (mic) 75% of b ...	1984	6744975
restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000.	a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ...	1985	3002238
restriction endonuclease mapping and cloning of mycobacterium fortuitum var. fortuitum plasmid pal5000.	a restriction map of mycobacterium fortuitum var. fortuitum plasmid pal5000 was established. the unique sites for apai, bamhi, bglii, bsteii, clai, ecori, ecorv, hpai, kpni and nari were located on the 5.0-kb plasmid. the plasmid had no sites for ahaiii, bcli, hindiii, psti, sphi and xbai. pal5000 was cloned into pbr322 and propagated in escherichia coli. three hybrid pal5000-pbr322 plasmids carrying the complete pal5000 sequence were constructed by joining the plasmids at their bamhi, ecori or ...	1985	3002238
[adaptive effect and withdrawal syndrome of propranolol in relation to norepinephrine and epinephrine].		1985	2934953
distribution of sensory receptors in the flexor carpi radialis muscle of the cat.	the structures and distribution of encapsulated muscle receptors were examined in serial transverse sections of flexor carpi radialis in the adult cat. four types of receptors (muscle spindles, golgi tendon organs, paciniform, and pacinian corpuscles) were identified. their structures resembled those encountered in other limb muscles. pacinian corpuscles were rare and occurred only in the external fascial coat of the muscle near its origin. the other three receptor types were distributed in an u ...	1985	3156250
non-immune igg f(ab')2 binding to group c and g streptococci is mediated by structures on gamma chains.	the present investigation was designed to determine whether the heavy or the light immunoglobulin chain is involved in the non-immune binding of igg f(ab')2 fragments to specific surface receptors on human group c and g streptococci. purified human polyclonal igg was mildly reduced with dithiothreitol and alkylated with iodoacetamide. light (l) and heavy (h) chains were separated. intact igg and purified l and h chains of polyclonal immunoglobulin g were tested in an inhibition assay for non-imm ...	1985	3156400
shigellosis in children--prevalence of subgroups and antibiotic resistance.		1985	3836974
on the importance of the level of glutathione and the activity of the pentose phosphate pathway in heat sensitivity and thermotolerance.	heating of ehrlich ascites tumour (eat) cells and mouse fibroblast lm cells to 43 or 44 degrees c respectively, results in an increased level of reduced glutathione (gsh). the maximum elevation in gsh was to 140 per cent for lm cells and to 120 per cent for eat cells. no increase of gsh in eat cells was observed after heating at 44 degrees c. lm cells were treated with diethylmaleate (dem) and the eat cells with buthionine-sulphoximine (bso) at non-toxic doses to deplete the levels of gsh. no ef ...	1985	3875580
estrogen receptors in human goitrous and neoplastic thyroid.	an attempt was made to quantify the number of specific estrogen binding sites in specimens of human goitrous or neoplastic thyroid tissue obtained by surgery. using synthetic estrogen [3h]moxestrol as a radioligand, the concentrations of estrogen receptors found were very low (average 0.57 +/- 0.47 fmol mg-1 of protein) approaching the detection limit. accordingly, no significant sex dependent differences in estrogen receptors could be revealed.	1985	3878776
identification of the phosphocarrier protein enzyme iiigut: essential component of the glucitol phosphotransferase system in salmonella typhimurium.	the phosphoenolpyruvate-dependent phosphorylation of glucitol has been shown to require four distinct proteins in salmonella typhimurium: two general energy-coupling proteins, enzyme i and hpr, and two glucitol-specific proteins, enzyme iigut and enzyme iiigut. the enzyme iigut was solubilized from the membrane and purified about 100-fold, free of the other protein constituents of the phosphotransferase system. enzyme iiigut was found in both the soluble and the membrane fractions. the soluble e ...	1985	3882659
[effect of the homogeneity of the culture, age and amount of the inoculation matter on glucoamylase biosynthesis by aspergillus niger strain b 77].		1985	3929570
intraventricular 6-hydroxydopamine increases thyrotropin-releasing hormone (trh) content in regions of rat brain.	rats were given intraventricular (ivt) injections of various doses (50-400 micrograms, hydrobromide salt) of 6-hydroxydopamine (6-ohda) and killed 1, 3 or 6 days later. brains were removed, dissected into 11 regions, and the thyrotropin-releasing hormone (trh) content of each region was measured by radioimmunoassay. 6-ohda (400 micrograms) caused significant elevations in the trh content of 6 regions: olfactory bulb, anterior cortex, brainstem, posterior cortex, hippocampus, and amygdala-pirifor ...	1985	3934717
characterization of nif regulatory genes in rhodopseudomonas capsulata using lac gene fusions.	translational fusions of the escherichia coli laczya operon to rhodopseudomonas capsulata nif genes were obtained by using mini-mudii1734 [castilho et al., j. bacteriol. 158 (1984) 488-495] inserts into cloned fragments of r. capsulata dna. a lac fusion to the nifh gene, which encodes dinitrogenase reductase, was used to classify nif- mutations occurring in regulatory genes. nine mutations were unable to activate nifhdk transcription. the nine mutations define four nif regulatory genes. three of ...	1985	3938422
hormone concentrations, mammary development and milk yield in goats given long-term bromocriptine treatment in pregnancy.	ten british saanen goats were treated daily with 5 mg bromocriptine intramuscularly from week 8 of pregnancy until week 20 (day 140). by comparison with untreated control goats (n = 8), concentrations of prolactin in plasma were suppressed throughout the treatment period and remained significantly lower until 3 days prepartum, parturition occurring on day 153 +/- 0.7 (mean +/- s.e.m., n = 10). growth hormone concentrations were low, but the incidence of levels exceeding 1 microgram/l was increas ...	1985	3968507
hormone concentrations, mammary development and milk yield in goats given long-term bromocriptine treatment in pregnancy.	ten british saanen goats were treated daily with 5 mg bromocriptine intramuscularly from week 8 of pregnancy until week 20 (day 140). by comparison with untreated control goats (n = 8), concentrations of prolactin in plasma were suppressed throughout the treatment period and remained significantly lower until 3 days prepartum, parturition occurring on day 153 +/- 0.7 (mean +/- s.e.m., n = 10). growth hormone concentrations were low, but the incidence of levels exceeding 1 microgram/l was increas ...	1985	3968507
evaluation of broth microdilution susceptibility results for anaerobic organisms by use of a rapid direct colony inoculum.	a direct colony inoculum suspension procedure was compared with the overnight suspension procedure recommended for the broth microdilution anaerobic commercial system (micro-media systems, inc., potomac, md.). six national committee for clinical laboratory standards-recommended quality control organisms, bacteroides fragilis atcc 25285, clostridium perfringens atcc 13124, bacteroides thetaiotaomicron atcc 29741, bacteroides vulgatus atcc 29327, peptococcus magnus atcc 29328, peptococcus asacchar ...	1985	3972999
studies on human low serum igd phenotype and gm markers.	the human "low serum igd phenotype" was studied by simultaneous gm typing and igd immunoassay of several populations. an association between gm (f+b+) haplotype and low human igd was confirmed and extended to the "low serum igd phenotype"--as defined from population distribution and genetic studies by dunnette et al. 1978. further, it was shown that black american sera determined by gm haplotype, had a similar percentage of "low serum igd phenotype" samples (16%) although they lacked the "associ ...	1985	3978847